@Preamble{
"\ifx \undefined \bioname \def \bioname #1{{{\em #1\/}}} \fi" #
"\ifx \undefined \booktitle \def \booktitle #1{{{\em #1}}} \fi" #
"\ifx \undefined \dbar \def \dbar {\leavevmode\raise0.2ex\hbox{--}\kern-0.5emd} \fi" #
"\ifx \undefined \eth \def \eth {d} \fi" #
"\ifx \undefined \gene \def \gene #1{{{\sf #1}}} \fi" #
"\ifx \undefined \geoname \def \geoname #1{{#1}} \fi" #
"\ifx \undefined \k \let \k = \c \fi" #
"\ifx \undefined \TM \def \TM {${}^{\sc TM}$} \fi" #
"\ifx \undefined \latinname \def \latinname #1{{{\em #1\/}}} \fi" #
"\ifx \undefined \mathcal \def \mathcal #1{{\cal #1}} \fi" #
"\ifx \undefined \pkg \def \pkg #1{{{\tt #1}}} \fi"
}
@String{ack-nhfb = "Nelson H. F. Beebe,
University of Utah,
Department of Mathematics, 110 LCB,
155 S 1400 E RM 233,
Salt Lake City, UT 84112-0090, USA,
Tel: +1 801 581 5254,
FAX: +1 801 581 4148,
e-mail: \path|[email protected]|,
\path|[email protected]|,
\path|[email protected]| (Internet),
URL: \path|https://www.math.utah.edu/~beebe/|"}
@String{j-FISHES = "Fishes"}
@Article{Gaemers:2016:TDE,
author = "Pieter A. M. Gaemers",
title = "Taxonomy, Distribution and Evolution of Trisopterine
{Gadidae} by Means of Otoliths and Other
Characteristics",
journal = j-FISHES,
volume = "1",
number = "1",
pages = "18--51",
day = "17",
month = jul,
year = "2016",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes1010018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Gaemers:2017:CPM}.",
URL = "https://www.mdpi.com/2410-3888/1/1/18",
abstract = "In a greater study of the recent fossil Gadidae, the
object of this paper is to better define the
trisopterine species and their relationships. The
taxonomy of the four recent species usually included in
the genus Trisopterus is further elaborated by means of
published and new data on their otoliths, by published
data on general external features and meristics of the
fishes, and their genetics. Fossil otoliths, from the
beginning of the Oligocene up to the present, reveal
much of their evolution and throw more light on their
relationships. Several succeeding and partly
overlapping lineages representing different genera are
recognized during this time interval. The genus
Neocolliolus Gaemers, 1976, for \bioname{Trisopterus
esmarkii} (Nilsson, 1855), is more firmly based. A new
genus, Allotrisopterus, is introduced for
\bioname{Trisopterus minutus} (Linnaeus, 1758). The
similarity with \bioname{Trisopterus capelanus}
(Lacep{\`e}de, 1800) is an example of convergent
evolution. The tribe Trisopterini Endo (2002) should
only contain Trisopterus, Allotrisopterus and
Neocolliolus as recent genera. Correct identification
of otoliths from fisheries research and from sea bottom
samples extends the knowledge of the present day
geographical distribution of T. capelanus and T. luscus
(Linnaeus, 1758). T. capelanus is also living along the
Atlantic coast of Portugal and at least up to and
including the R{\'\i}a de Arosa, Galicia, Spain. There
it can easily be mistaken for A. minutus that is also
living there. Otoliths of T. luscus have been
identified from the Evvo{\"\i}k{\'o}s Channel between
Euboia and the mainland of Greece, thus it must live
also in the Aegean Sea. Otoliths prove to be a powerful
tool in taxonomy, biogeography and evolution of
teleosts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Murzina:2016:LFA,
author = "Svetlana A. Murzina and Zinaida A. Nefedova and
Svetlana N. Pekkoeva and Tatiana R. Ruokolainen and
Pauli O. Ripatti and Andrey V. Semushin and Nina N.
Nemova",
title = "Lipids and Fatty Acids of the \geoname{White Sea}
Herring \bioname{Clupea pallasi marisalbi} {Berg}
({Clupeiformes, Clupeidae}) from Different Habitats of
the \geoname{White Sea}",
journal = j-FISHES,
volume = "1",
number = "1",
pages = "65--76",
day = "13",
month = oct,
year = "2016",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes1010065",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/1/1/65",
abstract = "Habitation conditions significantly affect the
physiological and biochemical state of aquatic
organisms, including the balance of lipids that
performs important functions in cellular metabolism.
The lipid and fatty acid profiles of White Sea herring
in Dvina, Kandalaksha, and Onega bays, and in the
waters of Tersky Shore (the mouth of the Varzuga River)
in autumn (after the fattening period) were studied.
Different methods --- thin-layer, high-performance
liquid and gas chromatography --- were used for this
purpose. Determined heterogeneity on studied parameters
of lipid metabolism of fish from different habitats is
associated with differences in trophic and hydrological
conditions in these bays. Variations of lipid
compositions of herring groups can be a reflection of
qualitative and quantitative strategies of biochemical
adaptations, aimed at compensating the lipid metabolism
of fish under different trophic--ecological conditions
of habitation in the White Sea.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Booth:2016:FAP,
author = "Mark A. Booth and D. Stewart Fielder",
title = "Fortification of an Aquafeed with Potassium Chloride
Does Not Improve Survival of Juvenile
\geoname{Australian} Snapper \bioname{Pagrus auratus}
Reared in Potassium Deficient Saline Groundwater",
journal = j-FISHES,
volume = "1",
number = "1",
pages = "52--64",
day = "09",
month = sep,
year = "2016",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes1010052",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/1/1/52",
abstract = "This study was done to determine if fortification of a
commercial aquafeed with KCl could improve the survival
of juvenile Australian snapper \bioname{Pagrus auratus}
reared in K + deficient saline groundwater (KDSGW;
{$<$5} mg K + L \minus 1). Experiment 1 (Exp. 1) tested
whether feeding an aquafeed fortified with zero, 25, or
50 g KCl kg \minus 1 for 6 days affected feed intake
and survival of fish transferred immediately from
estuarine water to KDSGW of the equivalent salinity (20
g\cdotp L \minus 1). Experiment 2 (Exp. 2) investigated
whether an aquafeed fortified with zero, 10, or 25 g
KCl kg \minus 1 affected survival, feed intake, and
growth rate (SGR) of snapper reared in KDSGW fortified
to have 40\% or 100\% the [K + ] of equivalent salinity
estuarine water (20 g\cdotp L \minus 1). The results of
Exp. 1 demonstrated there was no benefit of fortifying
aquafeed with KCl; fish transferred into KDSGW stopped
feeding and developed symptoms akin to tetany. Some
individuals also died and others became moribund. Exp.
1 was terminated according to animal care and ethics
guidelines. The results of Exp. 2 indicated the amount
of KCl added to the aquafeed did not affect survival,
feed intake, or food conversion ratio (FCR) of snapper,
irrespective of water treatment. However, SGR and FCR
was better when fish were reared in normal estuarine
water and KDSGW fortified to have 100\% the [K + ] of
equivalent salinity estuarine water. Our results
demonstrated that juvenile snapper were unable to
utilize the KCl added to the aquafeed and were probably
reliant on sequestering K + ions from the water column
in order to maintain functions involving hydromineral
homeostasis. Fortification of aquafeeds with KCl does
not ameliorate the negative effects of KDSWG on the
survival of juvenile snapper.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esteban:2016:WNJ,
author = "Maria Angeles Esteban",
title = "Welcome to the New Journal \bioname{Fishes}",
journal = j-FISHES,
volume = "1",
number = "1",
pages = "16--17",
day = "31",
month = may,
year = "2016",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes1010016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/1/1/16",
abstract = "This study was done to determine if fortification of a
commercial aquafeed with KCl could improve the survival
of juvenile Australian snapper \bioname{Pagrus auratus}
reared in K + deficient saline groundwater (KDSGW;
{$<$5} mg K + L \minus 1). Experiment 1 (Exp. 1) tested
whether feeding an aquafeed fortified with zero, 25, or
50 g KCl kg \minus 1 for 6 days affected feed intake
and survival of fish transferred immediately from
estuarine water to KDSGW of the equivalent salinity (20
g\cdotp L \minus 1). Experiment 2 (Exp. 2) investigated
whether an aquafeed fortified with zero, 10, or 25 g
KCl kg \minus 1 affected survival, feed intake, and
growth rate (SGR) of snapper reared in KDSGW fortified
to have 40\% or 100\% the [K + ] of equivalent salinity
estuarine water (20 g\cdotp L \minus 1). The results of
Exp. 1 demonstrated there was no benefit of fortifying
aquafeed with KCl; fish transferred into KDSGW stopped
feeding and developed symptoms akin to tetany. Some
individuals also died and others became moribund. Exp.
1 was terminated according to animal care and ethics
guidelines. The results of Exp. 2 indicated the amount
of KCl added to the aquafeed did not affect survival,
feed intake, or food conversion ratio (FCR) of snapper,
irrespective of water treatment. However, SGR and FCR
was better when fish were reared in normal estuarine
water and KDSGW fortified to have 100\% the [K + ] of
equivalent salinity estuarine water. Our results
demonstrated that juvenile snapper were unable to
utilize the KCl added to the aquafeed and were probably
reliant on sequestering K + ions from the water column
in order to maintain functions involving hydromineral
homeostasis. Fortification of aquafeeds with KCl does
not ameliorate the negative effects of KDSWG on the
survival of juvenile snapper.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Morphis:2016:AVA,
author = "Gregory Morphis and Aggeliki Kyriazopoulou and
Constantina Nasopoulou and Eleni Sioriki and
Constantinos A. Demopoulos and Ioannis Zabetakis",
title = "Assessment of the in Vitro Antithrombotic Properties
of Sardine (\bioname{Sardina pilchardus}) Fillet Lipids
and Cod Liver Oil",
journal = j-FISHES,
volume = "1",
number = "1",
pages = "1--15",
day = "28",
month = sep,
year = "2016",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes1010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/1/1/1",
abstract = "The aim of the current study was to compare the
biological activities of total polar lipids (TPL) and
thin-layer chromatography (TLC) polar lipid fractions
of sardine fillet and cod liver oil against
atherogenesis. TPL and TLC polar lipid fractions
obtained from these two sources were assessed for their
ability to inhibit the platelet-activating-factor
(PAF)-induced platelet aggregation (PAF-antagonists) or
to induce platelet aggregation (PAF-agonists), since
PAF plays a crucial role in the initiation and
development of atherosclerosis. This study focused on
the polar lipids since previous studies have underlined
that the antithrombotic properties of foodstuffs are
mainly attributed to polar lipid micro-constituents.
TPL of sardine fillet induced platelet aggregation,
while TPL of cod liver had a bimodal effect on
platelets. TLC polar lipid fractions of both samples
exhibited in vitro aggregatory and inhibitory activity
towards platelets. However, TLC sardine polar lipid
fractions showed stronger in vitro antithrombotic
activities than the cod liver oil ones. These data
constitute evidence of the putative contribution of
fish polar lipids against cardiovascular diseases,
underling firstly the beneficial effect of fish and
fish lipids as functional foodstuffs against
atherogenesis and secondly the more important role of
sardine polar lipids as opposed to cod liver oil.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez-Cardenas:2017:PCD,
author = "Leonardo Mart{\'\i}nez-C{\'a}rdenas and Carlos A.
{\'A}lvarez-Gonz{\'a}lez and Oscar U.
Hern{\'a}ndez-Almeida and Carlos A. Fr{\'\i}as-Quintana
and Jes{\'u}s T. Ponce-Palafox and Sergio
Castillo-Vargasmachuca",
title = "Partial Characterization of Digestive Proteases in the
Green Cichlid, \bioname{Cichlasoma beani}",
journal = j-FISHES,
volume = "2",
number = "1",
pages = "4",
day = "08",
month = mar,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2010004",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/1/4",
abstract = "This study undertakes the characterization of
digestive proteases in the juvenile green cichlid,
Cichlasoma beani. The results obtained showed a higher
activity of alkaline proteases (0.14 \pm 0.01 U mg
protein \minus 1) compared to acid proteases (0.07 \pm
0.01 U mg protein \minus 1) in this species. The
optimum temperature of the alkaline proteases was 65
\degree C and these enzymes were more thermostable to
temperature changes than the acid proteases,
characterized by an optimal temperature of 55 \degree
C. The pH optimum was 2 for acid proteases, and 11 for
alkaline proteases, which were also more stable to
changes in pH between 8 and 10. The use of specific
inhibitors showed an acid protease inhibition of 88\%
with pepstatin A as inhibitor. In the zymogram SDS-PAGE
analysis of alkaline proteases, five active fractions
were revealed, indicating the presence of serine
proteases. These results confirm that both alkaline and
acid proteases are involved in the digestion of C.
beani, and suggest that this species is omnivorous with
carnivorous tendencies. The present study contributes
to our knowledge about the digestive physiology of C.
beani, and can be applied towards improved
understanding of the kinds of protein sources that
could be used in the development of inerts diets.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Frias-Quintana:2017:UPS,
author = "Carlos A. Fr{\'\i}as-Quintana and Carlos A.
{\'A}lvarez-Gonz{\'a}lez and Dariel Tovar-Ram{\'\i}rez
and Rafael Mart{\'\i}nez-Garc{\'\i}a and Susana
Camarillo-Coop and Emyr Pe{\~n}a and Mario A. Galaviz",
title = "Use of Potato Starch in Diets of Tropical Gar
(\bioname{Atractosteus tropicus}, Gill 1863) Larvae",
journal = j-FISHES,
volume = "2",
number = "1",
pages = "3",
day = "07",
month = mar,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2010003",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/1/3",
abstract = "Tropical gar, \bioname{Atractosteus tropicus}, is a
carnivorous fish species from Southern M{\'e}xico with
high value and acceptance in local markets. Therefore,
the present study aims to spare proteins in diets for
larviculture of this species. An experiment was
performed using three different experimental diets with
increasing carbohydrate levels based on potato starch
(S) and decreasing protein content (P) and total
energy: 16\% S (16\% S--44\% P), 22\% S (22\% S--40\%
P) and 28\% S (28\% S--36\% P). Tropical gar larvae
(five days post-hatching, 2.1 mm average notochordal
length) were assessed for growth, survival, degree of
cannibalism, and digestive enzyme activities for 30
days. Highest growth and survival (24\%), as well as
the lowest cannibalism (33\%), was seen in larvae fed
the 28\% S diet, and these larvae also had the highest
lipase, amylase and glucosidase activities (0.28, 0.56
and 0.11 units, respectively). Protease activity
(alkaline protease, trypsin, chymotrypsin, and leucine
aminopeptidase) was higher in the larvae fed the 22\% S
diet than in those given the 16\% S and 28\% S diets.
In conclusion, dietary inclusion of potato starch at
28\% S enhanced growth, survival and some digestive
enzyme activities, and decreased cannibalism in the
larval gar. Potato starch could replace dietary protein
as a major source of energy for A. tropicus larvae,
thereby reducing the cost of diets.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Setiawati:2017:USS,
author = "Martiwi Diah Setiawati and Tasuku Tanaka",
title = "Utilization of Scatterplot Smoothers to Understand the
Environmental Preference of Bigeye Tuna in the Southern
Waters off {Java--Bali}: Satellite Remote Sensing
Approach",
journal = j-FISHES,
volume = "2",
number = "1",
pages = "2",
day = "09",
month = feb,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2010002",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/1/2",
abstract = "The southern waters off Java-Bali were recognized as
spawning and potential fishing ground for tuna species.
However, few studies have been conducted on this area.
In this paper, the environmental preference of bigeye
tuna was assessed based on catch data and three main
environmental satellite data; namely; sea surface
temperature (SST), sea surface chlorophyll (SSC), and
sea surface height deviation (SSHD). Then, the
relationship between bigeye tuna catches and
environmental satellite data was analyzed by using a
simplified method of the Generalized Additive Model
(GAM) which is called scatterplot smoothers. This
method is the forerunner of GAM and has not yet been
applied for fisheries analysis. The aim of this study
was to evaluate its performance for/in analyzing bigeye
tuna habitat preference. The result indicated that SST,
SSC, and SSHD had a high correlation with the bigeye
tuna's spatial patterns. Furthermore, spatial patterns
of bigeye tuna preference display typical
characteristics of low SST, low SSC, and low positive
SSHD as well as areas with extreme SSHD values, which
are almost the same results as those identified with
GAM analysis in the same study area.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Enyidi:2017:EFP,
author = "Uchechukwu D. Enyidi and Juhani Pirhonen and Juhani
Kettunen and Jouni Vielma",
title = "Effect of Feed Protein:Lipid Ratio on Growth
Parameters of \geoname{African} Catfish
\bioname{Clarias gariepinus} after Fish Meal
Substitution in the Diet with Bambaranut
(\bioname{Voandzeia subterranea}) Meal and Soybean
(\bioname{Glycine max}) Meal",
journal = j-FISHES,
volume = "2",
number = "1",
pages = "1",
day = "30",
month = jan,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:18 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/1/1",
abstract = "Fishmeal (FM) was substituted with soybean meal
(Glycine max) (SBM) and bambaranut meal (Voandzeia
subterranea) (BNM) in 10 experimental African catfish,
\bioname{Clarias gariepinus}, diets. Feed formulation
was designed using mixture model. The inclusion level
of the three protein ingredients varied between 0\% and
60\%. Remaining 40\% comprised of basal ingredients
kept constant for all 10 feeds. African catfish of
average initial weight 35.2 \pm 0.9 g were fed with one
of the treatment diets for 28 days. The protein:lipid
ratios of the diets (range 1.5--3.4:1) were used in
evaluating the feed utilization and growth of the fish.
We found that catfish performances were mainly
depending on ingredients and not the ratio itself. The
protein:lipid ratios in the diets made of plant
ingredients were high but SGR was low. Specific growth
rate (SGR) increased with the increase of feed FM
content, being ca. 5\% day\minus 1 with 60\% FM diet
but ca. 2\% day\minus 1 at both 60\% BNM and 60\% SBM
diets. SGR was similar (3.5\% day\minus 1) with diets
of 30\% BNM or SBM inclusion with 30\% FM. Feed
conversion ratio increased from below 0.6 of the 60\%
FM diet up to 1.5 (60\% SBM) and 1.7 (60\% BNM).
Protein efficiency ratio decreased linearly with
increasing FM substitution, but protein productive
value (PPV) was similar for catfish fed 60\% FM diet
and its 50\% substitution with BNM or SBM. These
results suggest that protein:lipid ratio cannot be used
in assessing nutritional performance if the source of
feed ingredient vary widely. However, these results
suggest that BNM can partly substitute FM and
completely replace SBM in the diets of African
catfish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Palm:2017:TAI,
author = "Harry W. Palm and Irfan Yulianto and Uwe Piatkowski",
title = "Trypanorhynch Assemblages Indicate Ecological and
Phylogenetical Attributes of Their Elasmobranch Final
Hosts",
journal = j-FISHES,
volume = "2",
number = "2",
pages = "8",
day = "17",
month = jun,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2020008",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/2/8",
abstract = "This study explores non-metric multidimensional
scaling (nMDS) as a tool for investigating parasites as
indicators of the elasmobranch biology. An attractive
feature of nMDS is its ability to allow
assemblage-level parasite data to be simultaneously
applied to questions of host biology. This method was
examined using the tapeworm order Trypanorhyncha
Diesing, 1863, which is known to be transmitted among
their hosts through the marine food web (via
predation), can unambiguously be identified in the
intermediate and final hosts, and has the potential as
an indicator of the host feeding biology. Our analyses
focused on trypanorhynch assemblages in elasmobranchs
as definitive hosts. The relationships between
trypanorhynch assemblages and the depth, feeding
ecology, habitat, and phylogeny for all sharks were
complex, but we found that depth distribution, diet
composition and habitat type were the major influencing
factors. Several species of sharks showed different
characters than known from their descriptions that
could be attributed to the change of shark behavior or
the trypanorhynch host path. The relationship between
the trypanorhynch assemblage and factors for
carcharhiniform species alone was more robust than for
all sharks. In the carcharhiniform analysis, the
relationship between habitat type and trypanorhynch
assemblage was most remarkable. Overlapping host
ecology was evident even in phylogenetically-distant
related hosts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sakihara:2017:MDD,
author = "Troy S. Sakihara and Atsuko Fukunaga and Kimberly A.
Peyton",
title = "Mugilids Display Distinct Trait-Mediated Patterns with
a Reinvasion of {Para} Grass \bioname{Urochloa mutica}
in a Tropical Estuary",
journal = j-FISHES,
volume = "2",
number = "2",
pages = "7",
day = "23",
month = may,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2020007",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/2/7",
abstract = "Aggressive invasions by species such as para grass
Urochloa mutica (Forssk.) are pervasive throughout the
tropics, but the impacts on estuarine habitats and
biota are poorly understood. After weed control was
reduced in a tropical estuary, U. mutica reinvaded this
area composed of two contiguous habitats: a shallow
pond and a deeper river. These habitats are especially
important for \bioname{Mugil cephalus}, a culturally
prized native mullet, and introduced Moolgarda engeli
not targeted in the fishery. We investigated the
potential impacts of U. mutica on juvenile and adult
mullets by comparing the catch per unit effort (CPUE)
of the two mullet species and the physiochemical
measurements from the two contiguous habitats during
different stages of its reinvasion. Overall, negative
effects on the CPUE of M. cephalus and M. engeli were
found as a result of the U. mutica reinvasion. The M.
cephalus CPUE was consistently higher in the shallow
pond habitat compared to the deeper river habitat. It
decreased after U. mutica was established, correlative
to the invasive grass canopies smothering the shallow
habitat favored by juveniles. The M. engeli CPUE
significantly declined during the initial periods of U.
mutica reinvasion, but did not decline further after
the grass became established. No differences in the M.
engeli CPUE between habitats were found, indicating
that M. engeli are habitat generalists. These findings
are among the first in an insular tropical estuary
demonstrating a negative relationship between an
invasive grass and relative abundances of two
confamiliar fish, while revealing distinct patterns
attributed to species-specific traits. Importantly, the
broad overlapping biogeographic distribution of M.
cephalus and U. mutica, and the negative relationship
between these species, raises widespread concern for
this mullet as a globally important fisheries
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Greenwood:2017:DSH,
author = "M. F. D. Greenwood",
title = "Distribution, Spread, and Habitat Predictability of a
Small, Invasive, Piscivorous Fish in an Important
Estuarine Fish Nursery",
journal = j-FISHES,
volume = "2",
number = "2",
pages = "6",
day = "13",
month = may,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2020006",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/2/6",
abstract = "Invasive species often cause negative ecological and
economic effects. Florida has {$>$20} established
invasive fish species but only seven exist in
saltwater. The present study examined
\bioname{Belonesox belizanus} (Pike Killifish), a
Central American euryhaline fish introduced to Tampa
Bay (west-central Florida) in the early 1990s, which
has quantifiably reduced populations of small-bodied
native fishes and may compete with prized sportfish
juveniles in estuarine nursery habitat. Long-term
monitoring revealed that B. belizanus occurs in
estuarine waterbodies along a 31-km stretch of the
bay's eastern fringe, with a second, smaller population
in two western tributaries. Spread rate was estimated
to be 5.5--13 km year \minus 1, intermediate among
invasive poeciliids. A novel implementation of boosted
regression tree modeling to assess B. belizanus habitat
predictability found greater probability of presence
with decreasing water depth and pH, whereas presence
tended to be greatest at polyhaline salinity. It is
hypothesized that B. belizanus distribution in Tampa
Bay is constrained by deep, seawall habitats acting as
ecological barriers. Further B. belizanus spread
therefore may be most likely to occur by human release
(from aquaria or bait buckets) or bird carry-off. Newly
restored tidal habitat within the current range
probably will be invaded quite quickly by B.
belizanus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mozanzadeh:2017:MRS,
author = "Mansour Torfi Mozanzadeh and Jasem G. Marammazi and
Morteza Yaghoubi and Naser Agh and Esmaeil Pagheh and
Enric Gisbert",
title = "Macronutrient Requirements of Silvery-Black Porgy
(\bioname{Sparidentex hasta}): a Comparison with Other
Farmed Sparid Species",
journal = j-FISHES,
volume = "2",
number = "2",
pages = "5",
day = "13",
month = may,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2020005",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/2/5",
abstract = "Silvery-black porgy (\bioname{Sparidentex hasta}) is
recognized as one of the most promising fish species
for aquaculture diversification in the Persian Gulf and
the Oman Sea regions. In this regard, S. hasta has
received considerable attention, and nutritional
studies focused on establishing the nutritional
requirements for improving diet formulation have been
conducted during recent years. Considering the results
from different dose--response nutritional studies on
macronutrient requirements conducted in this species,
it can be concluded that diets containing ca. 48\%
crude protein, 15\% crude lipid, 15\% carbohydrates and
20 KJ g \minus 1 gross energy are recommended for
on-growing S. hasta juveniles. In addition, the optimum
essential amino acid profile for this species
(expressed as g 16 g N \minus 1), should be
approximately arginine 5.3, lysine 6.0, threonine 5.2,
histidine 2.5, isoleucine 4.6, leucine 5.4, methionine
+ cysteine 4.0 (in a diet containing 0.6 cysteine),
phenylalanine + tyrosine 5.6 (in a diet containing 1.9
tyrosine), tryptophan 1.0 and valine 4.6. Moreover, the
optimum dietary n-3 long chain polyunsaturated fatty
acids and soybean lecithin are recommended to be 0.8\%
and 6\%, respectively. The maximum replacement of fish
meal with soy protein is recommended to be between
16.5\% and 27.3\%. In addition, different vegetal oil
sources are also recommended for partial and almost
complete replacement of fish oil in diets. Although the
nutritional requirements in terms of macronutrients
have been established under laboratory conditions, the
analysis of the available literature indicate that
future studies need to be conducted using a more
holistic approach under intensive farming conditions in
which different nutrients or additives need to be
tested under different rearing conditions for refining
nutrient requirements in this species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Romano:2017:WOC,
author = "Nicla Romano and Giuseppe Scapigliati and Luigi
Abelli",
title = "Water Oxygen Content Affects Distribution of {T} and
{B} Lymphocytes in Lymphoid Tissues of Farmed Sea Bass
(\bioname{Dicentrarchus labrax})",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "16",
day = "14",
month = sep,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/16",
abstract = "Intensive aquaculture systems have stimulated proper
management in order to limit stressful stocking
conditions and environmental impact. This should
improve fish wellness and counteract adverse water
characteristics such as high concentrations of carbon.
The oxygen can be considered a key factor since
critical conditions leading to chronic rise of blood
catecholamines can depress the number and
Oxygen-carrying capacity of erythrocytes. In this
research, we evaluated the effects of variation in
water oxygen (from 6 \pm 1 to 13 \pm 1 mg/L) on
lymphocytes density in sea bass by using flow cytometry
and immunohistochemistry. Gut, gill, and thymus T-cells
were significantly enhanced in percentage in
hyperoxygenated fish compared with normo-oxygenated
group, while Peripheral Blood Leukocytes (PBL), head
kidney, and spleen T-cells were not significantly
different. Contrarily, in PBL, head kidney and spleen
hyper-oxygenation treatment provoked an enhancement of
B lymphocyte percentages. The distribution of positive
T-cells in hyper-oxygenated fish varied in the organs
and followed the relative increments/decrements
observed in flow cytometry. This study remarks the
sensitivity of sea bass adaptive immunocytes to oxygen
concentration, evidenced by the modification of
parameters. Sea bass culture under high-flow water
recycling and at around 12--13 mg/L oxygen
concentrations is therefore suggested to enhance the
immune response capacity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gaemers:2017:RDT,
author = "Pieter A. M. Gaemers and Jan Y. Poulsen",
title = "Recognition and Distribution of Two \geoname{North
Atlantic} \bioname{Gadiculus} Species, \bioname{G.
argenteus} and \bioname{G. thori} ({Gadidae}), Based on
Otolith Morphology, Larval Pigmentation, Molecular
Evidence, Morphometrics and Meristics",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "15",
day = "29",
month = aug,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030015",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/15",
abstract = "The silvery pout genus Gadiculus consists of small
aberrant codfishes with several extinct and currently
only one recognized extant species. The oldest
representatives of a Gadiculus lineage known from
otoliths are Early Miocene in age. Fossil evidence has
showed Gadiculus to originate from older genera
diverging early from other true cods of the family
Gadidae. As adult specimens of different species have
been found to be highly similar and difficult to
distinguish based on meristic and morphometric data,
the number of species in this gadid genus has been
controversial since different larval morphotypes were
first discovered some 100 years ago. For almost 70
years, Gadiculus thori and Gadiculus argenteus have
been considered subspecies only, with a distribution in
the Northeast Atlantic Ocean including the
Mediterranean. In this study, we resolve the
long-standing issue of extant Gadiculus not being
monotypic. New results in the form of distinct adult
otoliths and molecular data unambiguously show two
species of Gadiculus present --- in agreement with
larval morphotypes. Morphometric, meristic and
molecular characters, as well as larval pigmentation
are discussed in addition to present and past
geographic distributions of the two taxa from
distributions of fossil otoliths. At present, the
cold-water species Gadiculus thori (northern silvery
pout) is distributed in cold-temperate and subarctic
latitudes in the Northeast Atlantic, including a new
range extension off Southeast Greenland. Gadiculus
argenteus (southern silvery pout) occurs in warmer
waters and is distributed in the warm-temperate East
Atlantic and Mediterranean. Fossil otoliths show that
both species often co-existed in the Mediterranean from
the Late Pliocene to the Middle Pleistocene.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kawase:2017:DES,
author = "Hiroshi Kawase and Ryo Mizuuchi and Hirofumi Shin and
Yuki Kitajima and Koh Hosoda and Masahiro Shimizu and
Daisuke Iwai and Shigeru Kondo",
title = "Discovery of an Earliest-Stage ``{Mystery Circle}''
and Development of the Structure Constructed by
Pufferfish, \bioname{Torquigener albomaculosus}
({Pisces: Tetraodontidae})",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "14",
day = "23",
month = aug,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030014",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/14",
abstract = "Male pufferfish (Torquigener albomaculosus) construct
``mystery circles'', geometric circular structures with
radially aligned peaks and valleys, on sandy seabeds
for reproduction. We discovered an earliest-stage
mystery circle composed of dozens of irregular
depressions. The structure changed to a primitive
circular form with radially aligned valleys and a
circular depression in the central area on day 2. The
number of valleys increased, and the radius of the
structure was much smaller than that of the final
structure. These features were observed from days 2 to
4, considered the early stage of construction. The
mystery circle had radially aligned peaks and valleys
with a central flat area, while the radius and the
differences in elevation between the peaks and valleys
were increasing. These features, which were observed
from days 5 to 8, were considered the middle stage of
construction. On day 9, the mystery circle was
completed. An irregular pattern was formed in the
central area, and the radially aligned peaks were
decorated with shell and coral fragments. These are the
first recorded observations of construction of a
mystery circle from the earliest stage.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Doring:2017:EFB,
author = "Julian D{\"o}ring and Maik Tiedemann and Moritz
St{\"a}bler and Hans Sloterdijk and Werner Ekau",
title = "\bioname{Ethmalosa fimbriata} ({Bowdich} 1825), a
Clupeid Fish That Exhibits Elevated Batch Fecundity in
Hypersaline Waters",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "13",
day = "18",
month = aug,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030013",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/13",
abstract = "Little is known about the concerted influence of
temperature and salinity on the fecundity of clupeid
fishes. Due to a globally changing climate, both
physical parameters might act as stressors, severely
affecting the reproductive potential of clupeid fish
populations inhabiting tropical estuaries. Differences
in relative batch fecundities, the gonado-somatic
index, and the condition index of bonga shad
(\bioname{Ethmalosa fimbriata}) were analysed in
individual females sampled at the Senegalese coast and
inside the inverse Sine Saloum estuary, where salinity
increases upstream in all seasons. Multiple linear
regression models on fecundity and gonadal energy
storage show that clupeids can adapt towards increasing
their reproductive investment at temperatures (26--30
\degree C) and salinities (42--51), which by far exceed
marine conditions, in an effort to maximize recruitment
success. This reproductive strategy, however, is
accompanied by a trade-off between reproductive effort
and somatic growth, which ultimately limits the
species' reproductive potential inside the estuary. The
observed high variability in batch fecundities might be
a viable mechanism to adjust to fluctuating and rather
extreme environmental conditions. Understanding the
spawning biology of exploited clupeid fishes in
drastically changing environments is crucial for
evaluating the reproductive potential of stocks at the
outer reach of their physiological performance curve.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dedman:2017:ASM,
author = "Simon Dedman and Rick Officer and Deirdre Brophy and
Maurice Clarke and David G. Reid",
title = "Advanced Spatial Modeling to Inform Management of
Data-Poor Juvenile and Adult Female Rays",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "12",
day = "04",
month = aug,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030012",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/12",
abstract = "Chronic overfishing has depleted numerous elasmobranch
stocks in the North East Atlantic, but addressing this
issue has been hampered by management complications and
lacking data. Spatial management approaches have thus
been advocated. This work presents a novel application
and further development of an advanced spatial modeling
technique to identify candidate nursery grounds and
spawning areas for conservation, by subsetting already
limited data. Boosted Regression Tree models are used
to predict abundance of juvenile and mature female
cuckoo (\bioname{Leucoraja naevus}), thornback
(\bioname{Raja clavata}), blonde (R aja brachyura), and
spotted (R aja montagui) rays in the Irish Sea using
fish survey data and data describing fishing pressure,
predation and environmental variables. Model-predicted
spatial abundance maps of these subsets reveal distinct
nuances in species distributions with greater
predictive power than maps of the whole stock. These
resulting maps are then integrated into a single easily
understood map using a novel approach, standardizing
and facilitating the spatial management of data-limited
fish stocks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gaemers:2017:CPM,
author = "Pieter A. M. Gaemers",
title = "Correction: {Pieter A. M. Gaemers. Taxonomy,
Distribution and Evolution of Trisopterine Gadidae by
Means of Otoliths and Other Characteristics.
\booktitle{Fishes} 2016, {\bf 1}, 18--51}",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "11",
day = "17",
month = jul,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030011",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Gaemers:2016:TDE}.",
URL = "https://www.mdpi.com/2410-3888/2/3/11",
abstract = "Chronic overfishing has depleted numerous elasmobranch
stocks in the North East Atlantic, but addressing this
issue has been hampered by management complications and
lacking data. Spatial management approaches have thus
been advocated. This work presents a novel application
and further development of an advanced spatial modeling
technique to identify candidate nursery grounds and
spawning areas for conservation, by subsetting already
limited data. Boosted Regression Tree models are used
to predict abundance of juvenile and mature female
cuckoo (\bioname{Leucoraja naevus}), thornback
(\bioname{Raja clavata}), blonde (R aja brachyura), and
spotted (R aja montagui) rays in the Irish Sea using
fish survey data and data describing fishing pressure,
predation and environmental variables. Model-predicted
spatial abundance maps of these subsets reveal distinct
nuances in species distributions with greater
predictive power than maps of the whole stock. These
resulting maps are then integrated into a single easily
understood map using a novel approach, standardizing
and facilitating the spatial management of data-limited
fish stocks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liao:2017:MRS,
author = "Qiuhong Liao and Tetsuhito Suzuki and Kohno Yasushi
and Dimas Firmanda Al Riza and Makoto Kuramoto and
Naoshi Kondo",
title = "Monitoring \geoname{Red Sea} Bream Scale Fluorescence
as a Freshness Indicator",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "10",
day = "10",
month = jul,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030010",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/10",
abstract = "Red sea bream (\bioname{Pagrus major}) scale
fluorescence characteristics were identified as a
potential rapid and non-destructive means for assessing
the fish's freshness. To investigate this, live red sea
breams were purchased, slaughtered, and prior to
measurement, stored at 22 \pm 2 \degree C for 27 h.
During subsequent storage, the K value of the dorsal
meat --- as a standard freshness indicator --- along
with front-face fluorescence spectra of representative
dorsal scales, were measured simultaneously at 3 h
intervals. Two major fluorescent peaks, A and B, were
identified with excitation and emission wavelength
pairs of 280/310 nm and 340/420 nm, which were mainly
contributed to by tyrosine and collagen, respectively.
Subsequent analysis showed that the fluorescence
intensity ratio of peak B to A (I B / I A) increased
linearly during storage (R 2 = 0.95) and is proposed as
a potential non-destructive index of fish freshness.
Thus, our results suggest that the fluorescence
characteristics of fish scales can be used to assess
fish carcass freshness during storage.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Oliveira:2017:PRD,
author = "Catarina C. V. Oliveira and Mayra I. Grano-Maldonado
and Rui A. Gon{\c{c}}alves and Paulo A. Frias and
Ant{\'o}nio V. Sykes",
title = "Preliminary Results on the Daily and Seasonal Rhythms
of Cuttlefish \bioname{Sepia officinalis} ({Linnaeus},
1758) Locomotor Activity in Captivity",
journal = j-FISHES,
volume = "2",
number = "3",
pages = "9",
day = "28",
month = jun,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2030009",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:19 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/3/9",
abstract = "This research was aimed at characterizing the daily
rhythms of locomotor activity of cuttlefish Sepia
officinalis (Linnaeus, 1758), a promising aquaculture
species, in a captive environment. Cuttlefish were
distributed within two tanks equipped with infrared
photocells, allowing the monitoring of locomotor
activity continuously from August to February. Results
showed a preference of cuttlefish for being active
during the night in summer (24.2 \pm 1.3 \degree C) and
in autumn (18.4 \pm 3.4 \degree C), with maximum
activity peaks observed, respectively, at 01:09 h and
21:47 h, in close relation with sunset time. In winter
(12.7 \pm 1.4 \degree C), this preference changed, with
higher levels of activity observed during the afternoon
in December (15:20 h) and in January (18:13 h), thus
suggesting this species as possessing a dualistic
behaviour. Levels of activity progressively decreased
during autumn and winter, likely related to low
temperatures. The present study offers new insights on
the behaviour of \bioname{Sepia officinalis},
presenting a variability of patterns across seasons,
regulated by environmental synchronizers; photoperiod
determining the activity periods, and temperature
modulating the levels of activity. Such information may
be a valuable tool for the improvement of cuttlefish
culture protocols in aquaculture, and consequently its
welfare.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garrido:2017:PRL,
author = "Diego Garrido and Diana B. Reis and Diego Orol and Rui
A. Gon{\c{c}}alves and M. Virginia Mart{\'\i}n and
Ant{\'o}nio V. Sykes and Covadonga Rodr{\'\i}guez and
Beatriz C. Felipe and Xiaodong Zheng and Luis Lagos and
Eduardo Almansa",
title = "Preliminary Results on Light Conditions Manipulation
in \bioname{Octopus vulgaris} ({Cuvier}, 1797)
Paralarval Rearing",
journal = j-FISHES,
volume = "2",
number = "4",
pages = "21",
day = "24",
month = nov,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2040021",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/4/21",
abstract = "High paralarvae mortality is a major bottleneck
currently hindering the control over the lifecycle of
common octopus (\bioname{Octopus vulgaris} Cuvier,
1797). It is believed that this problem might be
related to either zoo-technical and/or nutritional
aspects. The present paper is focused on the study of
different zoo-technical aspects related to light
conditions on the rearing of paralarvae, including the
effects of polarization in prey ingestion, the use of a
blue filter to simulate natural conditions, and the use
of focused light to avoid reflections of the rearing
tank's walls. In the first experiment, O. vulgaris
paralarvae ingestion of Artemia sp. and copepods (Tisbe
sp.) was assessed under either normal or polarized
light. In the second experiment, the effect of a blue
filter with natural light or focused artificial light
on growth and mortality was assessed over 15 days of
rearing. Ingestion rate was not influenced by light
polarization. Nonetheless, a significantly higher
ingestion of Artemia sp. with respect to copepods was
observed. The blue filter promoted the use of natural
light conditions in Octopus paralarval culture, while
focused light reduced the collision of the paralarvae
against the walls. However, no significant differences
were found in paralarval growth nor survival.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Caccia:2017:APE,
author = "Elisabetta Caccia and Maria Agnello and Marcello Ceci
and Patricia Strickler Dinglasan and Gerardo R. Vasta
and Nicla Romano",
title = "Antimicrobial Peptides Are Expressed during Early
Development of Zebrafish (\bioname{Danio rerio}) and
Are Inducible by Immune Challenge",
journal = j-FISHES,
volume = "2",
number = "4",
pages = "20",
day = "08",
month = nov,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2040020",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/4/20",
abstract = "Antimicrobial peptides (AMPS) are ancestral components
in the evolution of immunity from protozoans to
metazoans. Their expression can be constitutive or
inducible by infectious challenge. Although
characterized in detail in their structure and
activity, the temporal and spatial expression of AMPS
during vertebrate embryogenesis is still poorly
understood. In the present study, we identified
selected AMPs in zebrafish, and characterized their
expression during early development, and upon
experimental immune challenge in adult animals, with
the goal of establishing this genetically-tractable
model system for further AMP studies. By mining
available genomic databases, zebrafish AMP sequences
homologous to AMPs from other vertebrates were selected
for further study. These included parasin I and its
enzyme cathepsin D, \beta -defensin (DB1),
liver-expressed antimicrobial peptide 2 (LEAP2),
bactericidal permeability-increasing protein (BPI), and
chromogranin-A and -B (CgA and CgB). Specific primers
were designed for RT-PCR amplification of each AMP gene
of interest and amplicons between 242 bp and 504 bp
were obtained from RNA extracted from adult zebrafish.
Sequencing of the amplicons and alignment of their
deduced amino acid sequences with those from AMPs from
other vertebrate species confirmed their identity. The
temporal expression of AMPs was investigated by RT-PCR
analysis in fertilized oocytes, embryos, and adult
individuals. Parasin I and chatepsin D transcripts were
detectable immediately after fertilization, while the
transcripts for CgA and CgB became evident starting at
48 h post fertilization. Mature transcripts of LEAP2
and DB1 were detectable only in the adult zebrafish,
while BPI transcripts were detectable starting from the
12th day post fertilization. To explore the possible
upregulation of AMP expression by infectious challenge,
experiments were carried out in adult zebrafish by
intraperitoneal injection of a cocktail of
lipopolysaccharide and lipoteichoic acid. Except for
CgA and CgB, amplicons corresponding to all tested AMPs
showed stronger signals in the experimental animals as
compared to the unchallenged controls. This study
provided information on the early expression of AMPs in
zebrafish from ontogeny to adulthood and their
inducibility by microbials. This information could be
useful to actuate new prophylactic strategies as an
alternative to the use of antibiotics in culture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lerner:2017:RSX,
author = "Justin Lerner and Juan C. Levesque and Liana
Talaue-McManus",
title = "Recreational Swordfish (\bioname{Xiphias gladius})
Fishery: Angler Practices in {South Florida (USA)}",
journal = j-FISHES,
volume = "2",
number = "4",
pages = "18",
day = "27",
month = oct,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2040018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/4/18",
abstract = "The management of highly migratory species (HMS) is a
complex domestic and international system that was
initially established to regulate HMS taken in
commercial fisheries in the Atlantic Ocean. For
managing HMS taken in recreational fisheries, the
authority and the data required is lacking and remains
to be stipulated by regulating bodies. In the {United
States}, Florida saltwater anglers target various HMS,
but swordfish (\bioname{Xiphias gladius}) is a favorite
among anglers. The recreational swordfish fishery off
the Southeast Florida coast has experienced resurgence
in recent years, with directed tournaments resuming in
2001 after being absent almost 20 years. Today, South
Florida supports the largest group of recreational
swordfish anglers in the world. Despite the increasing
popularity and interest, little data is available
describing the recreational swordfish fishery and its
socio-economic aspects in South Florida. This study
aimed to compile, describe, and identify the
demographics, fishing tactics, costs, and fishery
management perceptions of recreational swordfish
anglers in South Florida based on nonprobability
purposive sampling organized through the Southeast
Swordfish Club (SESC). The sample size (n = 38)
represented about 16--38\% of the SESC members and
between 6\% and 8\% of the recreational anglers that
actively targeted swordfish in South Florida during the
time of the survey. We acknowledge the sample size was
small (n = 38), but believe the study encompassed the
most active swordfish anglers given their knowledge,
expertise, and connection with the fishery in terms of
participants, fishing effort, and fishing techniques.
As such, it is highly probable that a large portion of
the recreational swordfish angling population was
represented by members of the SESC in terms of
swordfishing gear, techniques, and socio-economics,
which reduced apparent bias in the study. Overall, the
annual income of recreational swordfish anglers in 2007
ranged from US \$15,000 to \$200,000 with an average
income of \$91,940 ($ n = 33$). Sixty-nine percent of
polled anglers indicated they had more than 26 years of
recreational fishing experience and 81\% had less than
10 years of experience targeting swordfish in South
Florida. Thirty-seven percent of surveyed anglers
indicated they departed from Port Everglades, Florida.
To target swordfish, anglers generally used five rods
and set their bait, commonly squid, at 91 m. Anglers
also indicated they changed their fishing tactics from
day to night, and took about five fishing trips per
month. Overall, anglers spent around \$14,210 on annual
costs associated with swordfishing, which was 16\% of
their annual income. Many polled anglers also reported
they were dissatisfied with the current swordfish
management regulations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Goncalves:2017:PIW,
author = "Renata Gon{\c{c}}alves and Maria Alexandra
Teod{\'o}sio and Joana Cruz and Radhouan Ben-Hamadou
and Ana Dulce Correia and Lu{\'\i}s Ch{\'\i}charo",
title = "Preliminary Insight into Winter Native Fish
Assemblages in \geoname{Guadiana Estuary} Salt Marshes
Coping with Environmental Variability and
Non-Indigenous Fish Introduction",
journal = j-FISHES,
volume = "2",
number = "4",
pages = "19",
day = "26",
month = oct,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2040019",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/4/19",
abstract = "This work aims to undertake a preliminary
characterization of winter fish assemblages in the salt
marsh areas of Guadiana lower estuary (South-East
Portugal) and discusses the potential risks of habitat
dominance by a non-indigenous species (NIS). To this
effect, six field campaigns were carried out in four
sampling sites during winter season targeting the
collection of fish species. A total of 48 samples were
collected. Individuals from seven different taxa
(marine and estuarine) were collected, although the
assemblage was dominated by two estuarine species ---
the native Pomatoschistus sp. (goby) and the NIS
\bioname{Fundulus heteroclitus} (mummichog). Goby was
the most abundant taxa in the majority of salt marsh
habitats, except for one specific, marsh pool, where
extreme environmental conditions were registered,
namely high temperature and salinity. Such conditions
may have boosted the intrusion of mummichog in this
area. This species is well adapted to a wide range of
abiotic factors enabling them to colonize habitats
where no predators inhabit. Impacts of mummichog
introduction in the Guadiana salt marsh area are still
unpredictable since this is the first time they have
been recorded in such high density. Nevertheless, in
scenarios of increased anthropogenic pressure and,
consequently, habitat degradation, there is a potential
risk of mummichog spreading to other habitats and
therefore competing for space and food resources with
native species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Enyidi:2017:CVP,
author = "Uchechukwu D. Enyidi",
title = "\bioname{Chlorella vulgaris} as Protein Source in the
Diets of \geoname{African} Catfish \bioname{Clarias
gariepinus}",
journal = j-FISHES,
volume = "2",
number = "4",
pages = "17",
day = "16",
month = oct,
year = "2017",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes2040017",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/2/4/17",
abstract = "Plant proteins substitutes of fishmeal in aquafeed are
usually lacking in some essential amino acids and fatty
acids. The microalgae \bioname{Chlorella vulgaris} has
good-quality protein with amino acids rich in
methionine, lysine and alanine. Four novel diets having
C. vulgaris as the main source of protein were produced
for African catfish \bioname{Clarias gariepinus} with
an initial average weight of 1.09 \pm 0.05 g. The diets
were labeled Feed 1 (F1) to feed 4 (F4). The treatment
diets were included 25\% (F1), 15\% (F2), 5\% (F3) and
0\% (F4) green algae meal. The basal ingredients of the
feed were corn (maize) included as F1, 40\%, F2, 43\%,
F3, 53\% and F4, 43\%; and millet meal, which varied in
F1 as 23\%, F2, 30\%, F3, 30\% and F4, 30\%. The
ingredients were preconditioned at 110 \degree C and
pelleted. Post-fingerling African catfish were stocked
at 10 fish per aquarium. There were three replicate
aquariums for each feed type and the fish were fed for
60 d. The specific growth rate was best for the catfish
fed with 25\% C. vulgaris diet 7.86 \pm 0\% day \minus
1, and worst at 6.77 \pm 0.07\% day \minus 1 for the
control group F4, 0\% algal meal. The food conversion
ratio (FCR) was lowest (1.88 \pm 0.02) for 25\% algal
meal diet (F1) and highest (2.98 \pm 0.01) for the 0\%
algal meal diet F4. Similarly, catfish had average
weight gain of 121.02 \pm 0.04 g for those fed with F1
compared to 62.50 \pm 0.0 g for those fed with 0\%
algae F4. Protein efficiency ratio was highest for the
F1-fed fish (2.46 \pm 0.22) and lowest for those fed
with F4 (2.02 \pm 0.09). The hepatosomatic index was
lowest for F1-fed fish (1.48 \pm 0.01) and highest for
catfish fed with F4 (2.50 \pm 0.59). Based on the
results, C. vulgaris is a good protein source for
African catfish and can also substitute fishmeal in the
catfish diets.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sharda:2018:STS,
author = "Sakshi Sharda and Emmanuelle Argenti and Kay Lucek",
title = "On the Status of Threespine Stickleback
(\bioname{Gasterosteus aculeatus} {Linnaeus} 1758) in
{Lake Bracciano, Italy}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "17",
day = "15",
month = mar,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010017",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/17",
abstract = "For many species, the Mediterranean region harbors
distinct lineages that are of conservation concerns.
However, many of these are threatened by habitat
degradation and by the introduction of non-native
species. Here, we assess the status of the native
threespine stickleback (\bioname{Gasterosteus
aculeatus}) in the Lake Bracciano region in Italy,
where stickleback have been historically present.
During a dedicated sampling campaign in summer 2015,
surveying the potential habitats that sticklebacks
commonly occupy, we could not confirm the presence of
this species but found introduced species to be often
most abundant. Stickleback are thus likely to either
have become extinct over the last decades or be on the
verge to extinction in the Lake Bracciano region.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pasaribu:2018:ENT,
author = "Wesly Pasaribu and Sukenda Sukenda and Sri Nuryati",
title = "The Efficacy of \geoname{Nile} Tilapia ({Oreochromis}
niloticus) Broodstock and Larval Immunization against
\bioname{Streptococcus agalactiae} and
\bioname{Aeromonas hydrophila}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "16",
day = "07",
month = mar,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/16",
abstract = "\bioname{Streptococcus agalactiae} and
\bioname{Aeromonas hydrophila} have been recognized as
the causative agents of mortality in tilapia larvae
with single infection and coinfection. The objective of
this study was to evaluate the efficacy of maternal
transfer and offspring protection from the immunization
of monovalent and bivalent vaccines on Nile tilapia
(\bioname{Oreochromis niloticus}) broodstock and larval
immunization. Four groups of broodstock were
intraperitoneally injected with formalin killed
whole-cells of S. agalactiae (Sa group), A. hydrophila
(Ah group), the bivalent mixed vaccine of them (Biv
group), and phosphate-buffered saline as a control (Pbs
group). Immunization of the larvae produced from
immunized broodstock with a bivalent vaccine (Biv1
group) and Pbs (Pbs1 group) was performed by immersion
at 20 days after hatch. Larvae produced from the Pbs
group were unvaccinated as the control (Pbs2 group).
Changes in the specific antibody and relative percent
survival were measured. The Sa and Ah groups that could
increase specific antibodies and protection against
pathogenic bacteria were challenged with the homologous
bacteria. The Biv group stimulated and protected
against both S. agalactiae and A. hydrophila. The
specific antibody of the Biv1 group was higher than the
Pbs1 and Pbs2 groups. The last observation in this
study showed that the relative percent survival of the
Biv group after challenged S. agalactiae, A.
hydrophila, and coinfection were 74.74 \pm 3.18\%,
73.81 \pm 8.58\%, and 71.48 \pm 5.70\%, respectively.
The use of bivalent vaccines on the broodstock and
larvae may be a strategy to reduce mortality in Nile
tilapia larvae caused by single pathogen infection of
S. agalactiae and A. hydrophila, or coinfection with
both S. agalactiae and \bioname{A. hydrophila}.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Das:2018:EFA,
author = "Mousumi Das and Ferdous Ibn Rahim and Md. Amzad
Hossain",
title = "Evaluation of Fresh \bioname{Azolla pinnata} as a
Low-Cost Supplemental Feed for {Thai} Silver Barb
\bioname{Barbonymus gonionotus}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "15",
day = "05",
month = mar,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010015",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/15",
abstract = "Aquatic fern Azolla pinnata comprises significant high
food value with a good proportion of protein, vitamins,
and minerals. This study was carried out to examine the
effect of fresh A. pinnata as a substitution of
commercial fish feed (CFF) for Thai silver barb
Barbonymus gonionotus. Post fingerlings of B.
gonionotus were reared in five treatments, labeled T 1
to T 5, by substituting 0\%, 25\%, 50\%, 75\%, and
100\% protein of CFF with A. pinnata protein (dry
matter basis) respectively for 56 days. The specific
growth rate, net production rate, protein efficiency
ratio, proximate composition, and overall conditions of
fish were not significantly varied between the fish
reared completely with CFF and 25\% substitution with
A. pinnata. However, a significantly higher profit rate
(431.49 USD ha \minus 1 56 day \minus 1) was calculated
for fish reared in T 2 than other treatments. In
contrast, there was a significant reduction of growth
and other parameters of the fish that were observed in
the case where more than 25\% CFF was substituted with
A. pinnata. The poorest performance was observed in
fish fed completely with A. pinnata, at T 5. Based on
the results, 25\% of CFF of Thai silver barb could be
substituted with fresh A. pinnata without significantly
lowering their growth and product quality and could
contribute significant to a higher profit margin.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hossain:2018:ASC,
author = "Mohammad Y. Hossain and Robert L. {Vadas, Jr.} and
Ramon Ruiz-Carus and Shams M. Galib",
title = "{Amazon} Sailfin Catfish \bioname{Pterygoplichthys
pardalis} ({Loricariidae}) in \geoname{Bangladesh}: a
Critical Review of Its Invasive Threat to Native and
Endemic Aquatic Species",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "14",
day = "22",
month = feb,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010014",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/14",
abstract = "The Amazon sailfin catfish \bioname{Pterygoplichthys
pardalis} is native to the Amazon River basin. It has
since expanded its range to North America, the
Caribbean, and more recently to South Asia, as with
some of its congeners. Our paper reviews recent data
from Bangladesh, clarifies taxonomic identification,
and examines the potential effects of P. pardalis and
congeneric species on local aquatic ecosystems and
elsewhere in South Asia. Range expansions there
probably reflect aquarium releases and escapes from
aquaculture farms, as exacerbated by dispersal due to
storm flooding and in impacted waters like irrigation
canals. Given their potential ecosystem-level effects,
we also review potential control mechanisms and human
use for these invasive loricariids.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Asnicar:2018:SGE,
author = "Davide Asnicar and Giedr{\.e} A{\v{s}}monait{\.e} and
Lina Birgersson and Charlotta Kvarnemo and Ola Svensson
and Joachim Sturve",
title = "Sand Goby --- an Ecologically Relevant Species for
Behavioural Ecotoxicology",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "13",
day = "20",
month = feb,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010013",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/13",
abstract = "Locomotion-based behavioural endpoints have been
suggested as suitable sublethal endpoints for human and
environmental hazard assessment, as well as for
biomonitoring applications. Larval stages of the sand
goby (\bioname{Pomatoschistus minutus}) possess a
number of attractive qualities for experimental testing
that make it a promising species in behavioural
ecotoxicology. Here, we present a study aimed at
developing a toolkit for using the sand goby as novel
species for ecotoxicological studies and using
locomotion as an alternative endpoint in toxicity
testing. Exposure to three contaminants (copper (Cu),
di-butyl phthalate (DBP) and perfluorooctanoic acid
(PFOA)) was tested in the early life stages of the sand
goby and the locomotion patterns of the larvae were
quantified using an automatic tracking system. In a
photo-motor test, sand goby larvae displayed
substantially higher activity in light than in dark
cycles. Furthermore, all tested compounds exerted
behavioural alterations, such as hypo- and
hyperactivity. Our experimental results show that sand
goby larvae produce robust and quantifiable locomotive
responses, which could be used within an
ecotoxicological context for assessing the behavioural
toxicity of environmental pollutants, with particular
relevance in the Nordic region. This study thus
suggests that sand goby larvae have potential as an
environmentally relevant species for behavioural
ecotoxicology, and as such offer an alternative to
standard model species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pagliarulo:2018:GSS,
author = "Caterina Pagliarulo and Daniela Sateriale and Elisa
Scioscia and Nunziatina De Tommasi and Roberta
Colicchio and Chiara Pagliuca and Elena Scaglione and
Japo Jussila and Jenny Makkonen and Paola Salvatore and
Marina Paolucci",
title = "Growth, Survival and Spore Formation of the Pathogenic
Aquatic Oomycete \bioname{Aphanomyces astaci} and
Fungus \bioname{Fusarium avenaceum} Are Inhibited by
\bioname{Zanthoxylum rhoifolium} Bark Extracts In
Vitro",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "12",
day = "15",
month = feb,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010012",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/12",
abstract = "This study aimed to evaluate the in vitro activity of
Zanthoxylum rhoifolium bark (Zr-b) extracts against
pathogenic aquatic oomycete/fungal isolates that cause
different diseases in native European crayfish
resulting in an elevated mortality rate and severe
economic repercussions. n -hexane, chloroform,
chloroform--methanol (9:1) and methanol extracts of
Zr-b were used to evaluate the antifungal activity
against the strain UEF88662 of Aphanomyces astaci
(oomycete) and the strain SMM2 of Fusarium avenaceum
(fungus). The anti-oomycete and antifungal activity was
quantitatively evaluated by growth, survival and
sporulation microbiological assays. The extracts tested
demonstrated a dose-dependent inhibitory effect on
oomycete and fungal growth and survival, as well as on
the production of oomycete and fungal spores. This work
presents alternatives for the treatment and prevention
of the spreading of \bioname{Aphanomyces astaci} and
Fusarium avenaceum, the etiological agents of the
diseases crayfish plague and brown spot disease,
respectively. The antifungal properties of Zanthoxylum
rhoifolium bark extracts warrant further research on
their use in the prevention and treatment of both
oomycete and fungal diseases. The antifungal properties
of Zanthoxylum rhoifolium bark extracts, shown in
vitro, indicate the possibility of their use in new
therapeutic and prophylactic strategies, providing
perspectives for the design of in vivo studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Svendsen:2018:EHA,
author = "Morten Bo S{\o}ndergaard Svendsen and Nikolaj Reducha
Andersen and Per Juel Hansen and John Fleng
Steffensen",
title = "Effects of Harmful Algal Blooms on Fish: Insights from
\bioname{Prymnesium parvum}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "11",
day = "14",
month = feb,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010011",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/11",
abstract = "Blooms of the planktonic alga \bioname{Prymnesium
parvum} pose a global threat, causing fish kills
worldwide. Early studies on the exposure of fish to P.
parvum indicate that toxic effects are related to gill
damage. The more strictly defined concept of adverse
outcome pathways has been suggested as a replacement
for the mode of action in toxicology studies. In this
study, rainbow trout (Onchorhyncus mykiss) were exposed
to P. parvum. During exposure, oxygen consumption was
determined by respirometry, and ventilation and
coughing rate were determined via video surveillance.
Per breath oxygen consumption was calculated to assess
the ventilation effort to obtain a unit of oxygen. A
second experiment monitored fish behavior to assess
recovery. The results indicated that oxygen consumption
initially increased, but on average fell below the
standard oxygen consumption at 70\% relative exposure.
Being a function of ventilation frequency and oxygen
consumption, the per breath oxygen consumption
decreased throughout exposure. Behavioral results
determined that short-term P. parvum exposure
subsequently caused the exposed fish to seek flow
refuge immediately and to a greater extent than
unexposed fish. The adverse outcome pathway of P.
parvum on rainbow trout is that P. parvum acts as a
gill irritant resulting in non-recoverable respiratory
failure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hushangi:2018:EPA,
author = "Ramtin Hushangi and Seyed Pezhman Hosseini Shekarabi",
title = "Effect of a Peracetic Acid-Based Disinfectant on
Growth, Hematology and Histology of Juvenile Rainbow
Trout (\bioname{Oncorhynchus mykiss})",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "10",
day = "06",
month = feb,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010010",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/10",
abstract = "The effects of a peracetic acid-based disinfectant
product (Aquastart \circledR) were assessed on some
hematological parameters, histological aspects and skin
bacterial load of rainbow trout, likewise bacterial
load of the rearing tank water. A total of 180 healthy
rainbow trout weighing 124.65 \pm 10 g were divided
into two groups, each in three replicates in
flow-through tanks. First group was exposed to
Aquastart at 8.9 ppm for 30 min and second group was
considered as the control. The fish were then reared
for 60 days prior to sampling for hematological and
histological studies. The lowest bacterial load level
in both water columns and trout skin were observed in
the treated trout (p {$<$} 0.05). Meanwhile, no
significant impact on growth performance was recorded
between treated and control fish. The immunocompetent
cells population size in control fish were
significantly lower than treated fish (p {$<$} 0.05).
Histologically, no evidence of abnormality was seen in
the gills, kidney, and liver tissues of treated fish.
These results showed that application of Aquastart at
8.9 ppm is safe for use in flow-through tanks farming
rainbow trout.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Casado:2018:ASG,
author = "Francisco Casado and Santiago Casado and Diana
Ceballos-Francisco and Mar{\'\i}a {\'A}ngeles Esteban",
title = "Assessment of the Scales of Gilthead Seabream
(\bioname{Sparus aurata} {L.}) by Image Analysis and
Atomic Force Microscopy",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "9",
day = "30",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010009",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/9",
abstract = "Understanding the protective role of fish skin is
critical to improving the development of aquaculture,
since skin is the main surface that separates the
animal from its always hazardous environment. Many
techniques have been used for its study, but certain
structural characteristics of fish skin still remain
not clearly understood. That is the case with scales,
which have always been attributed a mere protective
role, but which are proving to have more functions than
it was traditionally thought. To acquire a deeper
knowledge, scales from six different regions of
gilthead seabream (\bioname{Sparus aurata} {L.}) were
studied and measured by image analysis. Results prove
that scales from the base of the dorsal fin are larger
than those in other parts of the fish body while scales
from the peduncle are the smallest of the skin.
Furthermore, a technique relatively new in this field,
known as atomic force microscopy (AFM), was used to
obtain representations of the ultrastructure of the
scales and measure certain features such as the circuli
and the lines in the basal layer. The data obtained
allowed us to compare the height of circuli among the
different scales, showing that they were higher in
scales from the dorsum and the operculum. The present
results introduce a nanostructural model of the scales
of gilthead seabream that might serve as a useful
guideline for future studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez:2018:HKT,
author = "Danixa Mart{\'\i}nez and Juan Pablo Pontigo and
Francisco J. Morera and Alejandro. Ya{\~n}{\'e}z and
Luis Vargas-Chacoff",
title = "Head Kidney Transcriptome Analysis and
Characterization for the Sub-\geoname{Antarctic}
Notothenioid Fish \bioname{Eleginops maclovinus}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "8",
day = "25",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010008",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/8",
abstract = "This study describes de novo transcriptome sequencing
and annotation analyses for the head kidney of the
sub-Antarctic notothenioid fish \bioname{Eleginops
maclovinus}, a sister group of the Antarctic
notothenioid fish clade. Moreover, E. maclovinus is one
of the most eurythermal and euryhaline representatives
of the Notothenioidei suborder. RNA-seq data were
generated by the 454 GS Junior system, resulting in
11,207 contigs that were then assembled by the Genomic
Workbench CLC software. The transcriptome was annotated
by BLASTing each sequence against the universal,
non-redundant NCBI database (National Center for
Biotechnology Information) using the AUSTRAL-omics
computer cluster. A significant number of transcripts
related to innate and adaptive immunity were found in
the sequences, which could be used as references in
future immunological studies in E. maclovinus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Office:2018:ARF,
author = "Fishes Editorial Office",
title = "Acknowledgement to Reviewers of {{\booktitle{Fishes}}}
in 2017",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "7",
day = "25",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010007",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/7",
abstract = "This study describes de novo transcriptome sequencing
and annotation analyses for the head kidney of the
sub-Antarctic notothenioid fish \bioname{Eleginops
maclovinus}, a sister group of the Antarctic
notothenioid fish clade. Moreover, E. maclovinus is one
of the most eurythermal and euryhaline representatives
of the Notothenioidei suborder. RNA-seq data were
generated by the 454 GS Junior system, resulting in
11,207 contigs that were then assembled by the Genomic
Workbench CLC software. The transcriptome was annotated
by BLASTing each sequence against the universal,
non-redundant NCBI database (National Center for
Biotechnology Information) using the AUSTRAL-omics
computer cluster. A significant number of transcripts
related to innate and adaptive immunity were found in
the sequences, which could be used as references in
future immunological studies in E. maclovinus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gonzalez-Silvera:2018:EDT,
author = "Daniel Gonzalez-Silvera and Marcelino Herrera and
Inmaculada Gir{\'a}ldez and Mar{\'\i}a {\'A}ngeles
Esteban",
title = "Effects of the Dietary Tryptophan and Aspartate on the
Immune Response of Meagre (\bioname{Argyrosomus
regius}) after Stress",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "6",
day = "24",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010006",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/6",
abstract = "Stress is one of the main problems of farmed fish,
where it has been seen to result in a variety of
disorders and negative effects. It is considered very
important to minimize such effects and one option is
the use of dietary additives. In the present
experiment, meagre (\bioname{Argyrosomus regius})
specimens were fed three different diets: a control
diet consisting of a commercial feed, a
tryptophan-supplemented diet and an
aspartate-supplemented diet. For each diet, fish were
divided into three subgroups: non-stressed (resting),
exposed to crowding or air-exposed. Just before the
stress, and one and six hours post-stress, serum
samples were taken to study several immune parameters
(protease, antiprotease, peroxidase and lysozyme) and
the bactericidal activity against three fish-pathogenic
bacteria (Vibrio angillarum, \bioname{V. harveyi} and
\bioname{Photobacterium damselae} subsp. piscicida).
The results demonstrated that feeding the animals with
the supplemented diets modulates the seric immune
parameters, particularly when animals were stressed by
air exposure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Angradi:2018:FOR,
author = "Ted R. Angradi",
title = "A Field Observation of Rotational Feeding by
\bioname{Neogobius melanostomus}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "5",
day = "22",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010005",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/5",
abstract = "Neogobius melanostomus, the round goby, was recorded
by underwater video feeding on crushed dreissenid
mussels at a depth of 12 m in Georgian Bay of Lake
Huron, a Laurentian Great Lake. In the video, gobies
used rotational or twist feeding to tear away particles
from crushed mussels. At least 43 examples of this
feeding maneuver occur in the video. Up to 120 gobies m
\minus 2 were visible at a time in the video. Mean
standard length of gobies appearing in the video was 37
mm. Mean standard length of fish exhibiting twist
feeding was larger, 48 mm. Mean size of intact mussels
in visible clusters was about 10 $ \times $ 20 mm, a
size which exceeds the gape width of the largest gobies
observed in the video. \bioname{Neogobius melanostomus}
is known to use twisting to wrest small attached
mussels from the substrates which can be crushed by
their pharyngeal teeth. I surmise that the behavior
observed in the video is an opportunistic manifestation
of this inherent behavioral adaptation to overcome gap
limitation and exploit a temporary windfall of food.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Goncalves:2018:PEM,
author = "Rui A. Gon{\c{c}}alves and Marco Tarasco and Dian
Schatzmayr and Paulo Gavaia",
title = "Preliminary Evaluation of Moniliformin as a Potential
Threat for Teleosts",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "4",
day = "16",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010004",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/4",
abstract = "Aquaculture feed manufacturers and producers
increasingly recognize the importance of mycotoxins,
which contaminate plant-based meals used in compound
aquafeeds, and their potential to negatively impact
production. Though data on the worldwide occurrence of
legislated mycotoxins e.g., trichothecenes and
zearalenone (ZEN) are well documented, relatively
little information is available regarding other
mycotoxins also produced by Fusarium, notably
moniliformin (MON). Given that MON is known to affect
the survival, growth, skeletal formation and bone
mineralization in terrestrial species, its widespread
occurrence on maize and maize by-products typically
used in aquaculture makes it relevant to study these
parameters in teleost fish. In the present work we have
tested the effect of MON exposure on survival, bone
development and mineralization using zebrafish
(\bioname{Danio rerio}) as a model species and fish
derived osteo-chondroprogenitor cell line for in vitro
studies. Moniliformin exposure did not decrease bone
mineralization in zebrafish larvae or extracellular
matrix mineralization in the mineralogenic cell line
VSa13. Here, the minimal in vitro cytotoxicity
concentration was found to be 1000 $ \mu $ g L \minus 1
MON. Incidence of deformities was also not altered by
MON at the concentration tested (450 $ \mu $ g L \minus
1) although larval growth was affected, as shown by a
decrease in the standard length of exposed specimens at
20 days post fertilization. Survival decreased
significantly in larvae exposed to MON concentrations
higher than 900 \mu g L \minus 1. Influence of MON on
survival and growth might be relevant for aquaculture
industry. As MON is a water-soluble mycotoxin, its
leaching from feed is highly probable, so MON
assimilation into the surrounding aqueous environment
should also be considered. Tested levels in fish larvae
are within the reported occurrence levels of MON in
commercial feed and plant meals.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esteves:2018:OPC,
author = "Eduardo Esteves and Jaime An{\'\i}bal",
title = "Optimization of Processing Conditions of Traditional
Cured Tuna Loins --- Muxama",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "3",
day = "09",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010003",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/3",
abstract = "Muxama is a traditional highly-valued food product
prepared from dry-cured tuna loins in southern Portugal
and Spain. The production procedure has seen little
change over the last centuries. The muxama's stability
is due to reduced water activity. In addition, the
drying method has secondary effects on characteristics
of flavor, color, and the nutritional value of the
product. Our objectives were to describe the dynamics
of important physicochemical parameters such as
moisture content, water activity (a W), NaCl
concentration (as water--phase salt, Z NaCl), pH and
color, during the salting and drying stages of muxama
production, and to test the effect(s) of changes in the
traditional processing conditions followed in southern
Portugal, aiming at optimizing the production
procedure. The lowest values of moisture and a W and
highest Z NaCl obtained after drying tuna loins for
seven days at 20 \degree C exceeded the values reported
for commercial products and have impact on the
appearance (color) of the product. Therefore, drying
tuna loins at lower temperatures (ca. 14 \degree C) is
probably more appropriate. The results obtained in this
study could be used in the design of future experiments
at other conditions and to assess other quality
parameters, e.g., total volatile base nitrogen (TVB-N),
thiobarbituric acid reactive substances (TBA-RS),
microorganism abundance and sensory attributes, and
subsequent validation trials.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xenarios:2018:IRP,
author = "Stefanos Xenarios and Henrique Queiroga and Ana I.
Lilleb{\o} and Ana Aleixo",
title = "Introducing a Regulatory Policy Framework of Bait
Fishing in \geoname{European} Coastal Lagoons: the Case
of {Ria de Aveiro in Portugal}",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "2",
day = "05",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010002",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/2",
abstract = "The harvesting of bait through digging in coastal
mudflats is practiced for recreational and commercial
purposes in European coastal systems including the Ria
de Aveiro coastal lagoon on the northwest Atlantic
coast of Portugal. The scale of harvesting in the Ria
de Aveiro has recently increased due to the current
economic climate in Portugal, with targeting of the
polychaete, \bioname{Diopatra neapolitana} species or
``casulo'' as it is widely known in the Aveiro region.
The national authorities have attempted to control
casulo digging by issuing a regulation (Ordinance) in
2014 on the maximum daily catch limit to be caught by
each individual. The daily catch limit is intended to
represent the Maximum Sustainable Yield (MSY) for
casulo beyond which overfishing will occur. The
monitoring of the regulatory measures is expected to be
conducted through on-site inspections in the digging
areas. However, weak law enforcement was noticed, while
there is also controversy over the daily catch limit
(quota) stipulated by the Ordinance. To this end, the
current study attempted to assess digging activities
through remote monitoring and random inspections for a
better policy enforcement of the national regulation.
In addition, different harvesting scenarios were
employed through a simplified bioeconomic model to
attribute the current and future harvesting trends of
bait digging in Aveiro coastal lagoon. The study
findings indicate that remote monitoring coupled with
some onsite interviews could be a more effective
approach for the implementation of the current bait
digging policy. Further, the results point to a
distinctive discrepancy between the daily catch amount
(MSY) introduced by the national legislation and the
study findings which should be further scrutinized. The
diggers seem to have reached the sustainable harvest
identified by the present research. The current
economic hardship in Portugal and the low profitability
in similar employment sectors will possibly attract
more diggers and increase harvesting in the near
future. An increased harvest would likely trigger
overfishing of D. neapolitana with unknown consequences
for the population of the species as well as the
aquatic ecosystem. The socio-economic and environmental
effects are yet to be further clarified with more
detailed data and advanced modeling techniques to
ensure the sustainability of the activity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Takase:2018:CIG,
author = "Mai Takase and Hideki Ushio",
title = "Changes in Intestinal Gene Expression of Zebrafish
(\bioname{Danio rerio}) Related to Sterol Uptake and
Excretion upon $ \beta $-Sitosterol Administration",
journal = j-FISHES,
volume = "3",
number = "1",
pages = "1",
day = "04",
month = jan,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:20 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/1/1",
abstract = "Replacement of fishmeal with plant ingredients will
introduce not only plant oil and protein but also
phytosterol to the fish diet. Mammals strictly restrict
the uptake of phytosterol at intestinal epithelial
cells by regulating the gene expressions of sterol
uptake and excretion proteins; however, phytosterol is
found in the fish muscle and other organs. In order to
assess the ability of phytosterol uptake by the
intestinal epithelial cells of fish, no-sterol diet,
cholesterol-, and \beta -sitosterol-containing diet was
separately administered to zebrafish, and the relative
mRNA expressions related to sterol uptake and excretion
were evaluated. Gene expression of Niemann-Pick C1-like
protein 1 in the sitosterol-fed group was significantly
higher than that of the cholesterol-fed group (p {$<$}
0.05). The expression of apolipoprotein A-I gene was
also higher in the sitosterol-fed group than that in
the no-sterol and cholesterol-fed groups. The
expressions of ATP-binding cassette, sub-family G,
member 5 and 8, were significantly higher in the
sitosterol-fed group, compared to the no-sterol group.
Regarding the gene expression of ATP-binding cassette
sub-family A, member 1, the sitosterol-fed group showed
higher expression level compared to the other groups (p
{$<$} 0.01). These results suggest that fish should be
tolerant to phytosterols in contrast to mammals.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Valero:2018:IEI,
author = "Yulema Valero and Alberto Cuesta and Matteo Cammarata
and Mar{\'\i}a Angeles Esteban and Elena Chaves-Pozo",
title = "Immune-Endocrine Interactions in the Fish Gonad during
Infection: an Open Door to Vertical Transmission",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "24",
day = "13",
month = jun,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020024",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/24",
abstract = "The interaction between the immune and endocrine
systems has long been recognized in vertebrates. In
fish, it is known that the prevalence and intensity of
such infections are higher in males than in females and
probably related to sex steroid hormone levels. In
addition, the immune response in the fish gonad tissues
is specifically regulated to prevent infertility. This
condition is used by some pathogens to colonize the
fish gonad, evade the systemic immune response, and so
spread to the progeny. This review brings up to date
our knowledge concerning fish gonad immunity and its
regulation, immune-endocrine interactions, and how some
pathogens use this tissue to spread to the progeny
through vertical transmission. More specifically, we
will look at the case of the European sea bass and
nodavirus (NNV). Sea bass is a very susceptible fish
species to NNV infections, and this virus has been
associated to vertical transmission since it is
detected in gonad fluids as well as in testicular
gametes. In fact, sea bass immunity in the gonad is
regulated in a very different way to other target
tissues (brain and retina) or immune-relevant tissues
(head-kidney or spleen).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mensoor:2018:DHM,
author = "Montazer Mensoor and Ali Said",
title = "Determination of Heavy Metals in Freshwater Fishes of
the {Tigris River in Baghdad}",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "23",
day = "13",
month = jun,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020023",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/23",
abstract = "The presence of heavy metals in freshwater fish
represents a global public health issue. The current
study aimed to determine the heavy metal concentration
and toxicity in some freshwater fish species collected
from the Tigris River in Baghdad. Out of the many fish
species in Iraq, the current study selected the Genus
Barbus as it represents the most popular fish food in
Iraq. The sample included twenty fishes and the
selected sample locations covered two industrial areas
in Baghdad (one north of Baghdad and one south of
Baghdad). The levels of heavy metals were determined by
using an atomic absorption spectrophotometer (AAS). The
results showed that concentrations of heavy metals in
the sampled fishes exceeded the acceptable levels for
food sources for human consumption. The results of this
study showed high levels of cadmium and chromium levels
in the tissues of the selected fish sample. Cd and Cr
were among the highest concentrations and both exceeded
the World Health Organization and Food and Agriculture
Organization of the United Nations acceptable levels
for heavy metals in fishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hasan:2018:RBP,
author = "Tajmira Hasan and Md. Foysul Hossain and Md. Mamun and
Md. Jahangir Alam and Mohammad Abdus Salam and S. M.
Rafiquzzaman",
title = "Reproductive Biology of \bioname{Puntius sophore} in
\geoname{Bangladesh}",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "22",
day = "11",
month = jun,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020022",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/22",
abstract = "Pool barb (\bioname{Puntius sophore}) is an important
small indigenous species of Bangladesh, belonging to
the family Cyprinidae of the order Cypriniformes. The
main objectives of the present study were to identify
the breeding season of Pool barb through observation of
the gonadosomatic index (GSI), fecundity, oocytes
diameter, and gonadal histology of the species. GSI was
calculated and fecundity was determined by the
gravimetric method. Interestingly, the highest value of
GSI was 15.43 \pm 2.20 for the month of April at
Gazipur while it was 15.60 \pm 1.74 in June for
Jessore. In Gazipur, the highest fecundity (5053 \pm
878.27) was recorded in the month of April and the
highest fecundity (5433 \pm 968.26) was recorded in the
month of June for Jessore. Histology of the ovary of
Pool barb shows the presence of early and late
perinucleolar stage of oocytes during March indicating
immature oocyte. Histological data revealed that the
spawning season of Pool barb starts from March to July
with a peak during April to May for Gazipur region and
May to July for Jessore region. Finally, these results
can be concluded that breeding season may vary from
region to region due to environmental as well as other
factors.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ivanova:2018:WTU,
author = "Lada Ivanova and Haitham Tartor and S{\o}ren Grove and
Anja B. Kristoffersen and Silvio Uhlig",
title = "Workflow for the Targeted and Untargeted Detection of
Small Metabolites in Fish Skin Mucus",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "21",
day = "07",
month = jun,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020021",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/21",
abstract = "The skin mucus of fish is in permanent contact with
the aquatic environment. Data from the analysis of the
chemical composition of skin mucus could potentially be
used for monitoring the health status of the fish.
Knowledge about mucus composition or change in
composition over time could also contribute to
understanding the aetiology of certain diseases. The
objective of the present study was the development of a
workflow for non-invasive sampling of skin mucus from
farmed salmon (\bioname{Salmo salar}) for the targeted
and untargeted detection of small metabolites. Skin
mucus was either scraped off, wiped off using medical
wipes, or the mucus' water phase was absorbed using the
same type of medical wipes that was used for the wiping
method. Following a simple filtration step, the
obtained mucus samples were subjected to hydrophilic
interaction chromatography coupled to high-resolution
mass spectrometry. Post-acquisition processing included
the targeted analysis of 86 small metabolites, of which
up to 60 were detected in absorbed mucus. Untargeted
analysis of the mucus samples from equally treated
salmon revealed that the total variation of the
metabolome was lowest in absorbed mucus and highest in
the scraped mucus. Thus, future studies including
small-molecule metabolomics of skin mucus in fish would
benefit from a sampling regime employing absorption of
the water phase in order to minimize the bias related
to the sampling step. Furthermore, the absorption
method is also a less invasive approach allowing for
repetitive sampling within short time intervals.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Grano-Maldonado:2018:TSU,
author = "Mayra I. Grano-Maldonado and Juan Moreno-Navas and
Maria Amparo Rodriguez-Santiago",
title = "Transmission Strategies Used by \bioname{Gyrodactylus
gasterostei} ({Monogenea}) on Its Host, the
Three-Spined Stickleback \bioname{Gasterosteus
aculeatus}",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "20",
day = "29",
month = may,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020020",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/20",
abstract = "The monogenean Gyrodactylus gasterostei and its host,
the three-spined stickleback \bioname{Gasterosteus
aculeatus}, were employed as a model system to
investigate the influence of gyrodactylid maturity on
host transfer. Laboratory experiments included the
determination of maturity (presence of a male
copulatory organ) and reproductive status (presence of
a daughter parasite) of the transmitting worms.
Parasites with a male copulatory organ were more likely
to abandon the host and attempt a host transfer.
Additionally, in vivo video observation suggested that
detached worms employ water turbulence to assist their
effective transfer onto a new host.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Arndt:2018:SEM,
author = "Erik Arndt and Or Givan and Dor Edelist and Oren Sonin
and Jonathan Belmaker",
title = "Shifts in {Eastern Mediterranean} Fish Communities:
Abundance Changes, Trait Overlap, and Possible
Competition between Native and Non-Native Species",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "19",
day = "20",
month = apr,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020019",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/19",
abstract = "During the last few decades the fish community has
changed substantially along the Eastern Mediterranean
continental shelf, which is a hotspot of invasion by
species that had migrated via the Suez Canal. Trawl
data from the Israeli coast spanning two periods
(1990--1994 and 2008--2011) were compared to identify
species with substantial variation in their relative
abundance between the two periods. The aim of this
study was to examine if certain ecological traits
characterize fish species showing an increase or
decrease in relative abundance, and if non-indigenous
fishes with strongly increasing populations may have
caused the decline of native species with similar
habitats and diets. We found that the main predictors
of population trends were species length, habitat
affinity, and maximum depth, with larger and soft
bottom species displaying decreasing abundances.
Comparing native and non-indigenous fishes with similar
habitat and diet, we found a potential for competitive
impact of the Indo-Pacific \bioname{Plotosus lineatus}
and two Upeneus spp. on the native Mullus species.
However, competition with non-indigenous fishes could
not generally explain the dramatic decline of many
other native species between the two study periods.
Alternative causes, such as fishery pressure and
increasing water temperature, are discussed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Moyano:2018:ESI,
author = "Francisco Javier Moyano",
title = "Editorial for the Special Issue on Nutritional
Requirements in New Fish Species under Culture",
journal = j-FISHES,
volume = "3",
number = "2",
pages = "18",
day = "23",
month = mar,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3020018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/2/18",
abstract = "The worldwide interest in developing the culture of
nonconventional fish species determines the need to
increase knowledge in different aspects of their basic
physiology, as well as in the application of such
information into practical protocols to be used in
their feeding, reproduction, and general handling
\ldots{}",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lindseth:2018:USM,
author = "Adelaide V. Lindseth and Phillip S. Lobel",
title = "Underwater Soundscape Monitoring and Fish
Bioacoustics: a Review",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "36",
day = "12",
month = sep,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030036",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/36",
abstract = "Soundscape ecology is a rapidly growing field with
approximately 93\% of all scientific articles on this
topic having been published since 2010 (total about 610
publications since 1985). Current acoustic technology
is also advancing rapidly, enabling new devices with
voluminous data storage and automatic signal detection
to define sounds. Future uses of passive acoustic
monitoring (PAM) include biodiversity assessments,
monitoring habitat health, and locating spawning
fishes. This paper provides a review of ambient sound
and soundscape ecology, fish acoustic monitoring,
current recording and sampling methods used in
long-term PAM, and parameters/metrics used in acoustic
data analysis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jerez:2018:SIF,
author = "Salvador Jerez and Ioannis Fakriadis and Maria
Papadaki and M. Virginia Mart{\'\i}n and Juana Rosa
Cejas and Constantinos C. Mylonas",
title = "Spawning Induction of First-Generation ({F1}) Greater
Amberjack \bioname{Seriola dumerili} in the {Canary
Islands, Spain} Using {GnRHa} Delivery Systems",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "35",
day = "05",
month = sep,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030035",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/35",
abstract = "The development of a sustainable aquaculture industry
requires the closing of the life-cycle of a potential
species in captivity, and the establishment of breeding
selection programs using hatchery-produced breeding
stocks. The greater amberjack \bioname{Seriola
dumerili} is a cosmopolitan pelagic species that has
been considered as a good candidate for the species
diversification of aquaculture production in the
Mediterranean region. However, commercial production is
still very limited due to bottlenecks in reproduction,
larval rearing and management control during grow out.
The aim of the present study was to examine the
reproductive development of hatchery-produced greater
amberjack and to develop a spawning induction protocol
based on the use of gonadotropin releasing hormone
agonist (GnRHa) in a controlled-release delivery
system. The results showed that hatchery-produced
greater amberjack undergo normal gametogenesis and can
be induced to undergo maturation, ovulation and
spawning after multiple administrations of GnRHa
implants, over an extended spawning period lasting from
May to September in the Canary Islands, Spain. The use
of GnRHa-delivery systems resulted in multiple spawns
of fertilized and viable eggs. Egg production was high
and egg quality adequate for the implementation of
larval rearing for commercial purposes. The handling
required to administer the GnRHa implants during the
prolonged spawning season did not result in any
negative effect on the welfare and reproductive
performance of the fish based on evaluation of several
biochemical parameters. The developed reproduction
control method shows great potential to advance the
commercial production of greater amberjack, by enabling
the use of hatchery-produced broodstocks for further
breeding selection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barragan-Mendez:2018:AEC,
author = "Cristina Barrag{\'a}n-M{\'e}ndez and Fini
S{\'a}nchez-Garc{\'\i}a and Ignacio Sobrino and Juan
Miguel Mancera and Ignacio Ruiz-Jarabo",
title = "Air Exposure in Catshark (\bioname{Scyliorhinus
canicula}) Modify Muscle Texture Properties: a Pilot
Study",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "34",
day = "04",
month = sep,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030034",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/34",
abstract = "Sharks are captured by tons for human consumption.
Improving the quality of their meat will produce
fillets that may have a higher economic value in the
market, and thus be beneficial for the management of
this fishery. In other animal species destined for
human consumption, a negative relationship between
pre-slaughtering stress and meat quality has been
demonstrated. By studying the commercial small-spotted
catshark (\bioname{Scyliorhinus canicula}), this work
aimed at linking pre-slaughter handling of captured
sharks and muscle fillets quality. An experimental
group of adult and subadult living catsharks captured
by hand and exposed to air (for 18 min, which is the
minimum time this species is exposed to air in the
fishing deck during fisheries procedures), and an
undisturbed group, were evaluated. After air exposure,
catsharks returned to water for recovery. Muscle
lactate and water content were quantified after acute
exposure (for 18 min), 5 h and 24 h. This challenge
elicited stress responses in the muscle such as
increased lactate levels and immediate dehydration,
followed by recovery of lactate levels and
overhydration. Muscle consistency, a relevant variable
describing quality of seafood according to its ability
to be swallowed by the consumer, paralleled muscle
water content changes. The results indicated for the
first time that handling alive sharks exposed to air
results in muscle fillets with different texture
properties. Whether these changes in muscle texture
induce higher quality fillets has yet to be proven. Our
recommendation is to minimize time of air exposure
experienced by sharks when captured, including fast
slaughtering instead of leaving them to die by
asphyxia, as current on-board procedures.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jahangiri:2018:APW,
author = "Ladan Jahangiri and Mar{\'\i}a {\'A}ngeles Esteban",
title = "Administration of Probiotics in the Water in Finfish
Aquaculture Systems: a Review",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "33",
day = "22",
month = aug,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030033",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/33",
abstract = "Over the last few decades, the contribution of
aquaculture to animal protein production has increased
enormously, and the sector now provides almost half of
the fish and shellfish consumed worldwide, making it a
major food producer. Nevertheless, many factors,
including infections, pollution, and stress, may result
in significant economic losses. The aquaculture
industry will not be totally successful without the
therapeutic and preventive means to control all these
factors. Antibiotics (long used in aquaculture
practice) have tended to aggravate the problem by
increasing antibiotic resistance. Concomitantly,
probiotics have widely been suggested as eco-friendly
alternatives to antibiotics. However, the way in which
probiotics are applied in aquaculture is a key factor
in their favorable performance. The aim of this review
was to examine the current state of probiotics
administration through the water in finfish
aquaculture. The review also attempts to cover the
research gaps existing in our knowledge of this
administration mode, and to suggest the issues that
need to be investigated in greater depth.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Majumdar:2018:CPM,
author = "Bhaskar Chandra Majumdar and Faria Afrin and Md. Golam
Rasul and Dinesh Chandra Shaha and A. K. M. Azad Shah",
title = "Changes in Physicochemical, Microbiological, and
Sensory Properties of Sun-Dried \bioname{Mystus
vittatus} During Storage at Ambient Temperature",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "32",
day = "20",
month = aug,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030032",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/32",
abstract = "The objective of this study was to investigate the
changes in physicochemical, microbiological, and
sensory properties of sun-dried Mystus vittatus during
storage at ambient temperature. The fish was dried
under sun exposure on bamboo mats for 5--6 days until
the moisture content reduced to approximately 10--15\%.
The dried fish was then packed into airtight
polyethylene bags and stored at ambient temperature (24
to 29 {$^\circ $}C) for 90 days. The physical and
sensory properties revealed that the dried fish was
acceptable for human consumption for up to 60 days. The
pH value slightly decreased from 6.42 to 5.95 during
the storage period. The moisture content increased
significantly (p {$<$} 0.05), while no significant
difference was observed for the protein, lipid, and ash
contents (on a dry matter basis) with the increase in
the storage time. The peroxide value, acid value, and
conjugated dienes of the lipids increased significantly
during the storage period. The microbial load also
increased with the increase in the storage period. The
results of this study suggest that the product was
slightly oxidized during the storage period and could
be stored for up to two months.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Midway:2018:IGE,
author = "Stephen R. Midway and Andrew Ostrowski and Lindsey
West and Mario Hernandez and Matthew D. Robertson",
title = "Improved Growth Estimates for \bioname{Lethrinus
harak}: Measuring Increments, Adjusting Ages, and
Fitting Flexible Growth Models",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "31",
day = "14",
month = aug,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030031",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/31",
abstract = "Thumbprint emperor (Lethrinus harak) are a widely
distributed, tropical species ranging throughout the
Indo-Pacific region. In coastal Tanzania, overfishing
is likely occurring and thumbprint emperor are commonly
represented in catches. The goal of this study was to
estimate age and growth to provide basic life history
information that may help inform future management. We
sampled a total of n = 55 thumbprint emperors from both
fishery-dependent and fishery-independent sources.
Annular age estimates were improved with measurements
of otolith markings. Fish ages ranged from zero to five
years. We also evaluated the addition of otolith edge
size (a proxy for fractional age) to age estimation,
and fit two von Bertalanffy growth models---one for the
whole ages and one for the fractional ages---using a
flexible Bayesian framework. Growth parameters were
similar between the two models, and ultimately, L
{\infty} (maximum asymptotic size parameter) estimates
were comparable to other published values for the
species, although our estimates of K (growth
coefficient parameter) were smaller. Robust aging
techniques for tropical fishes can provide a foundation
for basic fishery management, which would help to
sustain the future of this widely distributed fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kampouris:2018:FRC,
author = "Thodoros E. Kampouris and Debora Milenkova and Ioannis
E. Batjakas",
title = "On the Finding of the Rare Crab \bioname{Paragalene
longicrura} ({Nardo}, 1868) ({Crustacea, Decapoda,
Brachyura, Progeryonidae}) from {Thermaikos Gulf,
Northwest Aegean Sea, Greece}",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "30",
day = "08",
month = aug,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030030",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/30",
abstract = "The finding of a rare crab, Paragalene longicrura, is
herein recorded from Thermaikos Gulf. This constitutes
the northmost record of the species in Greek waters.
The species is only known from sporadic records in the
eastern Mediterranean basin. Ecological remarks on the
habitat of P. longicrura and other decapod species are
described for the first time for the Mediterranean.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Simeon:2018:IAS,
author = "Benaya Meitasari Simeon and Efin Muttaqin and Ulfah
Mardhiah and Muhammad Ichsan and Dharmadi and Andhika
Prima Prasetyo and Fahmi and Irfan Yulianto",
title = "Increasing Abundance of Silky Sharks in the
\geoname{Eastern Indian Ocean}: Good News or a Reason
to be Cautious?",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "29",
day = "18",
month = jul,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030029",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/29",
abstract = "The silky shark (\bioname{Carcharhinus falciformis})
is the most common shark landed in Indonesian waters,
including in the Indian Ocean. However, even though
they are frequently caught, there is a lack of
information on the population and abundance of silky
sharks in the Indian Ocean. Cilacap and Tanjung Luar
are two of Indonesia's shark fishery hotspots, both
located on the edge of the Indian Ocean, which is a
part of the Indonesian Fisheries Management Area (FMA)
573. The fishers from both places usually have fishing
grounds in that particular fisheries management area.
This research aims to update the abundance, and to
investigate the factors, which influence catches of
silky shark in the FMA 573 based on monthly enumeration
during 2015--2016. This study found that standardized
catch-per-unit-effort (CPUE) or abundance indices of
silky shark in the Indonesian FMA 573 increased from
2015 to 2016. We suggested that fish immigration and
decreasing fishing pressure may affect the fish
abundance. Fisheries management is required to regulate
the fishing pressure, such as controlling the number of
boats/licenses and the number of trips or fishing gear,
as well as initializing spatial and temporal fishing
closure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tahar:2018:FWQ,
author = "Alexandre Tahar and Alan Kennedy and Richard D.
Fitzgerald and Eoghan Clifford and Neil Rowan",
title = "Full Water Quality Monitoring of a Traditional
Flow-Through Rainbow Trout Farm",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "28",
day = "17",
month = jul,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030028",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/28",
abstract = "Traditional freshwater rainbow trout farms are still
popular in some European countries such as Poland,
France and Ireland. These systems generally operate in
flow-through configuration. The impact such production
systems might have on water quality remains mostly
unknown. The present study was set up to fulfil this
objective of monitoring water quality on different fish
farm locations in order to identify the impacts of the
whole farm (comparison of farm inlet and outlet) and at
pond scale in order to understand the water quality
dynamics and to better understand the impact of
multiple water reuse (water passes) in a given pond on
water quality. In the absence of any sort of water
treatment, an increase in the number of water passes
was shown to create an increase in ammonium
concentration along the farm. Finally, this traditional
flow-through rainbow trout production system was
revealed to almost be at its full carrying capacity
with respect to internal water quality parameters and
fish welfare. To increase fish production, some water
treatment techniques (solid/liquid separation,
nitrification) would have to be introduced in order to
optimize the rearing water quality for fish growth and
to minimize the release of pollutants in the receiving
water to limit the impact on the environment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nieves-Rodriguez:2018:EGD,
author = "Karen N. Nieves-Rodr{\'\i}guez and Carlos Alfonso
{\'A}lvarez-Gonz{\'a}lez and Emyr S.
Pe{\~n}a-Mar{\'\i}n and Fernando Vega-Villasante and
Rafael Mart{\'\i}nez-Garc{\'\i}a and Susana
Camarillo-Coop and Dariel Tovar-Ram{\'\i}rez and Laura
T. Guzm{\'a}n-Villanueva and Karl B. Andree and Enric
Gisbert",
title = "Effect of $ \beta $-Glucans in Diets on Growth,
Survival, Digestive Enzyme Activity, and Immune System
and Intestinal Barrier Gene Expression for Tropical Gar
(\bioname{Atractosteus tropicus}) Juveniles",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "27",
day = "10",
month = jul,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030027",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/27",
abstract = "The application of {\beta}-1,3/1,6-glucan derived from
yeast at five concentrations (0\%, 0.5\%, 1.0\%, 1.5\%,
and 2.0\%) in formulated diets was evaluated in
juveniles for its effects on the growth, survival,
digestive enzymatic activity, and expression of genes
associated with the immune system (interlukin-10
(IL-10), transforming growth factor (TGF), occludin
(OCC), mucin2 (MUC2), lysozyme (LYS), and
nucleotide-binding and oligomerization domain 2 (NOD2))
in tropical gar (\bioname{Atractosteus tropicus}). For
the experiment, three replicates of 30 fish per
experimental unit (70 L) were cultivated for 62 days.
The growth results showed no statistically significant
differences in relation to weight and total length
between treatments. The activity of digestive enzymes
(alkaline proteases, trypsin, leucine aminopeptidase,
and amylase) did not show significant differences
between treatments, except for chymotrypsin activity,
where fish fed 1.0\% and 1.5\% of {\beta}-glucans
showed higher activities compared with the rest of the
treatments. On the other hand, the analysis of gene
expression did not show significant differences between
treatments, although a tendency of increase in the
expression of IL-10, TGF, MUC2, and OCC was observed
with an addition of 1.5\% of the prebiotic, but there
was a decrease in the fish fed with 2\% of the
prebiotic. It is possible to include concentrations of
between 0.5\% and 1.5\% of {\beta}-glucans in the diets
for A. tropicus, with no detectable adverse effects on
growth, survival, digestive enzyme activity, or
specific gene expression. {\beta}-glucan 1,3/1,6 added
at 1.0\% and 1.5\% in the diet significantly increases
chymotrypsin activity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kitasato:2018:ADB,
author = "Airi Kitasato and Tomo Miyazaki and Yoshihiro Sugaya
and Shinichiro Omachi",
title = "Automatic Discrimination between \bioname{Scomber
japonicus} and \bioname{Scomber australasicus} by
Geometric and Texture Features",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "26",
day = "27",
month = jun,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030026",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/26",
abstract = "This paper proposes a method for automatic
discrimination of two mackerel species:
\bioname{Scomber japonicus} (chub mackerel) and
\bioname{Scomber australasicus} (blue mackerel).
Because S. japonicus has a much higher market price
than S. australasicus, the two species must be properly
sorted before shipment, but their similar appearance
makes discrimination difficult. These species can be
effectively distinguished using the ratio of the base
length between the dorsal fin's first and ninth spines
to the fork length. However, manual measurement of this
ratio is time-consuming and reduces fish freshness. The
proposed technique instead uses image processing to
measure these lengths. We were able to successfully
discriminate between the two species using the ratio as
a geometric feature, in combination with several
texture features. We then quantitatively verified the
effectiveness of the proposed method and demonstrated
that it is highly accurate in classifying mackerel.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rasul:2018:BMS,
author = "Md. Golam Rasul and Bhaskar Chandra Majumdar and Faria
Afrin and Mohammad Abu Jafor Bapary and A. K. M. Azad
Shah",
title = "Biochemical, Microbiological, and Sensory Properties
of Dried Silver Carp (\bioname{Hypophthalmichthys
molitrix}) Influenced by Various Drying Methods",
journal = j-FISHES,
volume = "3",
number = "3",
pages = "25",
day = "25",
month = jun,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3030025",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/3/25",
abstract = "An experiment was performed to evaluate the effects of
different drying methods with different pre-treatments
on the physico-chemical, microbiological, and sensory
properties of the silver carp,
\bioname{Hypophthalmichthys molitrix}. In order to
achieve this objective, the collected fresh fish were
dried using traditional (without any pre-treatment),
improved (fish soaked in a 5\% salt solution for 10 min
and then treated with chili powder (0.3\%) and turmeric
powder (0.3\%)), and solar tunnel (fish soaked in a 5\%
salt solution for 10 min) drying methods. The results
showed that the dried fish produced by the solar tunnel
drying method were rehydrated more rapidly than the
products produced by the traditional and improved
drying methods. The moisture content of the dried H.
molitrix was varied from 18.24 to 25.43\% on a fresh
matter basis. There was no significant (p $>$ 0.05)
difference found regarding the protein, lipid, and ash
content among the different drying methods on a dry
matter basis. The total volatile base nitrogen (TVB-N)
contents ranged between 37.58 and 45.03 mg/100 g, and
significantly (p {$<$} 0.05) the lowest TVB-N was
observed in dried fish produced by the solar tunnel
drying method. Moreover, the highest peroxide and acid
values were found in the traditionally produced dried
fish as compared with the fish dried by the improved
and solar tunnel drying methods. The aerobic plate
count of dried fish ranged from 4.52 to 7.51 log CFU/g.
The sensory evaluation results revealed that the
solar-dried products showed superior quality than the
products produced by the traditional and improved
drying methods. The results revealed that the dried
fish produced by the solar tunnel drying method
provided the best product in terms of physico-chemical,
microbiological, and sensory aspects.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{vonSiebenthal:2018:TOU,
author = "Elena Wernicke von Siebenthal and Kristina Rehberger
and Christyn Bailey and Albert Ros and Elio L. Herzog
and Helmut Segner",
title = "Trade-Offs Underwater: Physiological Plasticity of
Rainbow Trout (\bioname{Oncorhynchus mykiss})
Confronted by Multiple Stressors",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "49",
day = "16",
month = dec,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040049",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/49",
abstract = "Organisms have evolved mechanisms to partition the
available resources between fitness-relevant
physiological functions. Organisms possess phenotypic
plasticity to acclimate to changing environmental
conditions. However, this comes at a cost that can
cause negative correlations or ``trade-offs'', whereby
increasing investments in one function lead to
decreased investments in another function. The aim of
the present study was to investigate the prioritization
of resource allocation between growth, pathogen
defense, and contaminant response in juvenile rainbow
trout (\bioname{Oncorhynchus mykiss}) exposed to
changes of resource income or expenditure. We performed
a multifactorial experiment with three
resource-impacting stressors --- limited food
availability, a parasitic infection, exposure to a
vitellogenesis-inducing contaminant --- and
combinations thereof. Treatment with the individual
stressors evoked the expected responses in the
respective physiological target systems --- body
growth, immune system, and hepatic vitellogenin
transcription --- but we found little evidence for
significant negative relations (trade-offs) between the
three systems. This also applied to fish exposed to
combinations of the stressors. This high phenotypic
flexibility of trout in their resource allocation
suggests that linear resource allocations as mechanisms
of phenotypic plasticity may be too simplistic, but it
also may point to a greater capacity of ectothermic
than endothermic vertebrates to maintain key
physiological processes under competing resource needs
due to lower maintenance costs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ruiz-Jarabo:2018:NRE,
author = "Ignacio Ruiz-Jarabo and Pura M{\'a}rquez and Luis
Vargas-Chacoff and Juan Antonio Martos-Sitcha and
Salvador C{\'a}rdenas and Juan Miguel Mancera",
title = "Narrowing the Range of Environmental Salinities Where
Juvenile Meagre (\bioname{Argyrosomus regius}) Can Be
Cultured Based on an Osmoregulatory Pilot Study",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "48",
day = "13",
month = dec,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040048",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/48",
abstract = "Aquaculture in Europe aims to diversify and optimize
fish farming. The meagre (\bioname{Argyrosomus regius})
arose as a promising species due to its fast growth
rates and flesh quality. Thus, it is currently being
produced in several Mediterranean countries, mainly in
sea-cages and salt-marshes. However, although meagre
naturally spend the first years of life in brackish
waters, to date it is cultured in seawater. Here, we
show that juveniles may not successfully face either
freshwater or hyper-osmotic environments as high as 55
ppt salinity. We found that 55 ppt induced catabolism
and mobilization of energy metabolites stored in the
liver, probably to maintain its osmotic balance.
Furthermore, we found that osmoregulatory tissues such
as gills managed to maintain plasma osmolality levels
without differences in meagre acclimated at 5, 12 and
39 ppt salinity. Our results demonstrate the euryhaline
capacity of this species, highlighting that juveniles
may be cultured in a wider range of salinities rather
than just at seawater. Future studies should focus on
optimal environmental salinities for the growth of A.
regius juveniles, including long-term experiments
limited to the range of 5 ppt to full-strength
seawater. Minimizing fish energy consumption in
osmoregulation could be economically beneficial for the
aquaculture industry in Europe.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luer:2018:PHH,
author = "Carl A. Luer and Catherine J. Walsh",
title = "Potential Human Health Applications from Marine
Biomedical Research with Elasmobranch Fishes",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "47",
day = "06",
month = dec,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040047",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/47",
abstract = "Members of the subclass of fishes collectively known
as elasmobranchs (Class Chondrichthyes, Subclass
Elasmobranchii) include sharks, skates, rays,
guitarfish, and sawfish. Having diverged from the main
line of vertebrate evolution some 400 million years
ago, these fishes have continued to be successful in
our ever-changing oceans. Much of their success must be
attributed to their uncanny ability to remain healthy.
Based on decades of basic research, some of their
secrets may be very close to benefitting man. In this
short review, some of the molecular and cellular
biological areas that show promise for potential human
applications are presented. With a brief background and
current status of relevant research, these topics
include development of new antibiotics and novel
treatments for cancer, macular degeneration, viral
pathogens, and Parkinson's disease; potentially useful
genomic information from shark transcriptomes; shark
antibody-derived drug delivery systems; and immune
cell-derived compounds as potential cancer therapeutic
agents.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Trifonova:2018:CAB,
author = "Oxana P. Trifonova and Dmitry L. Maslov and Anton N.
Mikhailov and Konstantin V. Zolotarev and Kirill V.
Nakhod and Valeriya I. Nakhod and Nataliya F. Belyaeva
and Marina V. Mikhailova and Petr G. Lokhov and
Alexander I. Archakov",
title = "Comparative Analysis of the Blood Plasma Metabolome of
Negligible, Gradual and Rapidly Ageing Fishes",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "46",
day = "04",
month = dec,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040046",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/46",
abstract = "There are a number of different animals that belong to
long- and short-lived species and show a various rate
of ageing, providing an ideal model to investigate
mechanisms of longevity. In this work, a metabolome
profiling of blood plasma from fishes with various
ageing rates---negligible (Pike \bioname{Esox lucius}
and Sterlet \bioname{Acipenser ruthenus}), gradual
(Zander \bioname{Sander lucioperca} and Perch
\bioname{Perca fluviatilis}) and rapid (Chum Salmon
\bioname{Oncorhynchus keta} and Pink Salmon
\bioname{Oncorhynchus gorbuscha})---was assessed by
means of direct infusion to quadrupole time-of-flight
mass spectrometry. Of the 2056 distinct m/z features
detected by a mass spectrometry metabolic profiling of
blood plasma samples, fifteen metabolites in the
classes of dipeptides, fatty acids, glycerolipids,
phosphoethanolamines and phosphatidylcholines were
significantly associated with ageing rate, independent
of species differences. This is the first study of the
metabolome of fishes with various ageing rate, and this
untargeted approach highlighted the metabolic
conditions that may serve to assess the ageing
process.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Reading:2018:OEQ,
author = "Benjamin J. Reading and Linnea K. Andersen and
Yong-Woon Ryu and Yuji Mushirobira and Takashi Todo and
Naoshi Hiramatsu",
title = "Oogenesis and Egg Quality in Finfish: Yolk Formation
and Other Factors Influencing Female Fertility",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "45",
day = "21",
month = nov,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040045",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/45",
abstract = "Egg quality in fishes has been a topic of research in
aquaculture and fisheries for decades as it represents
an important life history trait and is critical for
captive propagation and successful recruitment. A major
factor influencing egg quality is proper yolk
formation, as most fishes are oviparous and the
developing offspring are entirely dependent on stored
egg yolk for nutritional sustenance. These maternally
derived nutrients consist of proteins, carbohydrates,
lipids, vitamins, minerals, and ions that are
transported from the liver to the ovary by lipoprotein
particles including vitellogenins. The yolk composition
may be influenced by broodstock diet, husbandry, and
other intrinsic and extrinsic conditions. In addition,
a number of other maternal factors that may influence
egg quality also are stored in eggs, such as gene
transcripts, that direct early embryonic development.
Dysfunctional regulation of gene or protein expression
may lead to poor quality eggs and failure to thrive
within hours of fertilization. These gene transcripts
may provide important markers as their expression
levels may be used to screen broodstock for potential
spawning success. In addition to such intrinsic
factors, stress may lead to ovarian atresia or
reproductive failure and can impact fish behavior,
fecundity, and ovulation rate. Finally, postovulatory
aging may occur when eggs become overripe and the fish
fails to spawn in a timely fashion, leading to low
fertility, often encountered during manual strip
spawning of fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hussain:2018:OPR,
author = "Md. Ashraf Hussain and Md. Lutful Kabir and Md. Abu
Sayeed and A. T. M. Mahbub-E-Elahi and Md. Sultan Ahmed
and Md Jakiul Islam",
title = "Organochlorine Pesticide Residues and Microbiological
Quality Assessment of Dried Barb, \bioname{Puntius
sophore}, from the Northeastern Part of
\geoname{Bangladesh}",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "44",
day = "09",
month = nov,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040044",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/44",
abstract = "The present study was carried out in the northeastern
part of Bangladesh to investigate organochlorine
pesticide (OCP) residues in and microbiological quality
of dried barb (\bioname{Puntius sophore}). Samples were
collected from both producers and retailers from
December 2016 to April 2017. A control sample was also
prepared in the laboratory with the same raw fish used
by the producers to compare the results. Gas
chromatography with electron capture detector (GC-ECD)
was used to detect and quantify OCP residues. Six
samples out of 27 (about 22\%) were found to be
contaminated with OCP residues. Among these six
adulterated samples, four were from retailers and two
from producers. Only aldrin was detected in four
samples, and in the other two samples both aldrin +
dieldrin and aldrin + endrin were detected. Aldrin was
found in quantities between 0.332 and 0.967 ppm,
dieldrin 0.762 ppm, and endrin 0.828 ppm. All these
values were much higher than the maximum residual limit
(MRL) of 0.1 ppm. Total plate count (TPC) of producer
samples ranged from 5.3 {\pm} 0.02 log cfu g {-1} to
5.4 {\pm} 0.03 log cfu g {-1} and 6.2 {\pm} 0.02 log
cfu g {-1} to 6.4 {\pm} 0.02 log cfu g {-1} for
retailer samples and 5.0 {\pm} 0.03 log cfu g {-1} to
5.2 {\pm} 0.04 log cfu g {-1} for control samples.
Fungal count ranged from 3.2 {\pm} 0.04 log cfu g {-1}
to 3.5 {\pm} 0.04 log cfu g {-1}, 3.4 {\pm} 0.04 log
cfu g {-1} to 3.6 {\pm} 0.03 log cfu g {-1}, and 2.2
{\pm} 0.05 log cfu g {-1} to 2.5 {\pm} 0.03 log cfu g
{-1} for producer, retailer, and control samples,
respectively. All the producer and retailer samples and
one-third of the control samples were found to be
contaminated with \bioname{Escherichia coli}, whereas
Salmonella spp. were detected in amounts of 13.3\% in
producer samples and 20\% in retailer samples and none
in the control. In case of Vibrio spp., maximum count
was found in retailer samples (13.3\%), whereas
producer and control samples showed none. The findings
of the present study show that the presence of
pesticides and poor microbiological quality of dried
barb are alarming for consumers in Bangladesh and might
cause prolonged disease and impending longstanding risk
to human health.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Papadaki:2018:DAR,
author = "Maria Papadaki and Jos{\'e} Benito Peleteiro and
Blanca Alvarez-Bl{\'a}zquez and Jos{\'e} Luis
Rodr{\'\i}guez Villanueva and Fatima Linares and
Antonio Vilar and Evaristo P{\'e}rez Rial and Nuria
Lluch and Ioannis Fakriadis and Irini Sigelaki and
Constantinos C. Mylonas",
title = "Description of the Annual Reproductive Cycle of
Wreckfish \bioname{Polyprion americanus} in Captivity",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "43",
day = "19",
month = oct,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040043",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/43",
abstract = "Successful spontaneous reproduction and the production
of viable offspring of wild fish in captivity can take
some years of adaptation, and may also involve
different environmental conditions, sex ratios and
densities compared to natural populations. We followed
the reproductive cycle of wreckfish \bioname{Polyprion
americanus} ---a late maturing, deep-sea benthic
species---in captivity in three different broodstocks
in Spain under natural photoperiod and temperature, and
one broodstock in Greece under a constant temperature
of 16 {$^\circ $}C for two consecutive years, to
describe the reproductive cycle of the species and the
associated sex steroid hormone profiles. Oogenesis
begun in the fall and post-vitellogenic oocytes of 1250
{\mu}m were present between March and June. Males were
in spermiation condition and produced good-quality
sperm throughout the year, regardless of the
temperature profile to which they were exposed. Some
females completed oogenesis, underwent oocyte
maturation, and spawned spontaneously under both
constant and fluctuating temperatures. The sex steroid
hormones of both males and females followed the
already-known profiles during fish gametogenesis,
except for 17,20{\beta}-dihydroxy-progesterone, which
did not seem to be related to either female or male
maturation. The female reproductive dysfunctions that
were identified included (a) the arrest of oogenesis at
the cortical alveoli stage in certain females; (b) the
failure to undergo oocyte maturation in others; and (c)
the production of large percentages of unfertilized
eggs from the females that spawned spontaneously. Our
study suggests that reproduction in captivity is
possible in wreckfish maintained under typical marine
aquaculture conditions, but that reliable spawning and
production of high-quality eggs may require some years
of adaptation to captivity, before the reproductive
dysfunctions will be overcome.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Axen:2018:OMA,
author = "Charlotte Ax{\'e}n and Niccol{\`o} Vendramin and Anna
Toffan",
title = "Outbreak of Mortality Associated with Acipenser
Iridovirus \geoname{European} ({AcIV-E}) Detection in
{Siberian} Sturgeon (\bioname{Acipenser baerii}) Farmed
in {Sweden}",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "42",
day = "16",
month = oct,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040042",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/42",
abstract = "Infectious disease is a major challenge in aquaculture
and poses a constraint for the development of farming
of new species. In 2017, Siberian sturgeon
(\bioname{Acipenser baerii}) juveniles were imported
from Italy to a Swedish farm. Transport conditions were
suboptimal. Thirty percent died during transport and
within the first days after arrival. Ten days after
arrival, mortalities started to occur again, which
prompted initiation of an investigation into the
mortalities. Diseased fish were transported live to the
National Veterinary Institute (SVA) for necropsy and
further analysis. Pathological and histopathological
investigation was conducted. Virology was performed on
gills and internal organs by cell culture isolation and
using specific PCR protocols against nervous necrosis
virus (NNV) and Acipenser iridovirus European (AcIV-E).
The juveniles displayed neurological signs such as
lethargy, inability to maintain an upright position,
and erratic swimming. Body condition was below normal,
and gills were pale. One fish had petechial hemorrhages
on the abdomen and the snout. Two specimens had
intestinal hyperemia. Ventricles were air-filled, and
swim bladders were deflated. Viral cell cultures gave
negative results, but PCR analysis of gills and
internal organs detected the presence of AcIV-E. We
conclude that AcIV-E was associated with disease and
high mortality in the sturgeon juveniles, and stress
probably aggravated the course of the infection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Reverter:2018:BER,
author = "Miriam Reverter and Nathalie Tapissier-Bontemps and
David Lecchini and Bernard Banaigs and Pierre Sasal",
title = "Biological and Ecological Roles of External Fish
Mucus: a Review",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "41",
day = "09",
month = oct,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040041",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/41",
abstract = "Fish mucus layers are the main surface of exchange
between fish and the environment, and they possess
important biological and ecological functions. Fish
mucus research is increasing rapidly, along with the
development of high-throughput techniques, which allow
the simultaneous study of numerous genes and molecules,
enabling a deeper understanding of the fish mucus
composition and its functions. Fish mucus plays a major
role against fish infections, and research has mostly
focused on the study of fish mucus bioactive molecules
(e.g., antimicrobial peptides and immune-related
molecules) and associated microbiota due to their
potential in aquaculture and human medicine. However,
external fish mucus surfaces also play important roles
in social relationships between conspecifics (fish
shoaling, spawning synchronisation, suitable habitat
finding, or alarm signals) and in interspecific
interactions such as prey-predator relationships,
parasite--host interactions, and symbiosis. This
article reviews the biological and ecological roles of
external (gills and skin) fish mucus, discussing its
importance in fish protection against pathogens and in
intra and interspecific interactions. We also discuss
the advances that ``omics'' sciences are bringing into
the fish mucus research and their importance in
studying the fish mucus composition and functions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lindseth:2018:ALL,
author = "Adelaide V. Lindseth and Phillip S. Lobel",
title = "Addendum: {Lindseth, A. and Lobel, P. S. Underwater
Soundscape Monitoring and Fish Bioacoustics: a Review.
{{\booktitle{Fishes}}} 2018, {\bf 3}, 36}",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "40",
day = "08",
month = oct,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040040",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/40",
abstract = "Fish mucus layers are the main surface of exchange
between fish and the environment, and they possess
important biological and ecological functions. Fish
mucus research is increasing rapidly, along with the
development of high-throughput techniques, which allow
the simultaneous study of numerous genes and molecules,
enabling a deeper understanding of the fish mucus
composition and its functions. Fish mucus plays a major
role against fish infections, and research has mostly
focused on the study of fish mucus bioactive molecules
(e.g., antimicrobial peptides and immune-related
molecules) and associated microbiota due to their
potential in aquaculture and human medicine. However,
external fish mucus surfaces also play important roles
in social relationships between conspecifics (fish
shoaling, spawning synchronisation, suitable habitat
finding, or alarm signals) and in interspecific
interactions such as prey-predator relationships,
parasite--host interactions, and symbiosis. This
article reviews the biological and ecological roles of
external (gills and skin) fish mucus, discussing its
importance in fish protection against pathogens and in
intra and interspecific interactions. We also discuss
the advances that ``omics'' sciences are bringing into
the fish mucus research and their importance in
studying the fish mucus composition and functions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esquivel-Muelbert:2018:AVR,
author = "Juan R. Esquivel-Muelbert and Luisa Fontoura and
{\'E}verton Zardo and Danilo P. {Streit, Jr.} and
Adriane Esquivel-Muelbert and Juan R. E. Garcia",
title = "Assessing the Viability of Reintroduction of Locally
Extinct Migratory Fish \bioname{Brycon orbignyanus}:
Successful Growth, Dispersal and Maturation",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "39",
day = "01",
month = oct,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040039",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/39",
abstract = "The reintroduction of threatened fish species in areas
where wild populations have been depleted due to
anthropogenic impacts is an increasingly popular
conservation tool and mitigation policy. Despite the
importance of fish reintroduction for conservation
purposes, little is known about its efficiency. Here,
we assessed the viability of reintroduction of the
endangered migratory fish, Brycon orbignyanus, in an
area of the Upper Uruguay River basin where the species
has not been reported for more than 30 years. We
released 4000 yearling juveniles in the Pelotas River
in 2014 and maintained 400 juveniles in captivity as a
control population. After three years, a total of 13
individuals was recaptured, of which, 10 were
considered sexually mature with first maturation being
recorded in animals larger than 42 cm in total body
length. The age--length comparison with a control
population growth curve showed that recaptured fish
were slightly bigger than those in captivity.
Furthermore, important ecological attributes as
schooling behavior and dispersal capacity were recorded
for all recaptured individuals. Combined, our results
suggest that the re-establishment of a self-sustained
population of locally extinct species B. orbignyanus in
the Pelotas River may be successful if sustained over
time and supported by conservation policies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Naslund:2018:RMB,
author = "Joacim N{\"a}slund",
title = "Relative Mass of Brain- and Intestinal Tissue in
Juvenile Brown Trout: No Long-Term Effects of
Compensatory Growth; with Additional Notes on Emerging
Sex-Differences",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "38",
day = "28",
month = sep,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040038",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/38",
abstract = "This study investigated whether compensatory growth
causes long-term effects in relative brain- or
intestine size in a wild, predominantly anadromous,
population of brown trout (\bioname{Salmo trutta}). The
subject fish belonged to two treatment groups; one
group had undergone starvation and subsequent growth
compensation, while the other were unrestricted
controls. The main hypothesis that compensatory growth
would negatively affect brain and intestinal size, as a
consequence of growth trade-offs during the
compensatory phase, could not be supported as no
significant differences were detected between the
treatment groups. Further exploratory analyses
suggested that males and females started to diverge in
both brain and intestine size at around 130 mm fork
length, with females developing relatively smaller
brains and larger intestines. The size at which the
differences appear is a typical size for smoltification
(saltwater preadaptation), and females tend to smoltify
to a higher proportion than males. Smoltification is
known to cause a more elongated morphology and
relatively smaller heads in salmonids, and the marine
lifestyle is associated with rapid growth, which could
require relatively larger intestines. Hence, these
emerging sex differences could be a consequence of
sex-biased smoltification rates. An investigation of
wild smolts of both sexes indicated no differences in
brain or intestine mass between male and female
smolts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lacy:2018:IRT,
author = "Shaw Lacy and Fernando Ugalde and Luca Mao",
title = "Invasive Rainbow Trout (\bioname{Oncorhynchus mykiss})
Are Not Affected by Different Land Uses in a Multi-Use,
\geoname{Mediterranean} Climate Landscape",
journal = j-FISHES,
volume = "3",
number = "4",
pages = "37",
day = "26",
month = sep,
year = "2018",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes3040037",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:21 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/3/4/37",
abstract = "Land use carries implications for freshwater fish
conservation. Plantation forestry practices have been
shown to have negative impacts on resident fish fauna,
but little work has been conducted to assess these
impacts on invasive vs. native fish populations. Ten
headwater catchments in the Mediterranean climate zone
of Chile were used to assess the impacts of land use
(pine plantations vs. native forests) on fish condition
(length--weight relationship) and abundance
(catch-per-unit-effort (CPUE)) of the invasive trout
\bioname{Oncorhynchus mykiss} and the threatened native
catfish Nematogenys inermis. Negative impacts on trout
condition were associated with a lack of canopy cover
and river topology. The presence of N. inermis was
associated with catchment factors less favourable to
trout. Current environmental regulations and forestry
management practices do not appear to create negative
pressures on invasive trout from land use practices,
despite expectations from the literature. Assessing how
land use management regulations impact invasive and
native fishes should be a part of species conservation
and territorial planning.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Woodward:2019:EIA,
author = "Melanie A. Woodward and Lucy A. Winder and Penelope J.
Watt",
title = "Enrichment Increases Aggression in Zebrafish",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "22",
day = "19",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010022",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/22",
abstract = "Environmental enrichment, or the enhancement of an
animal's surroundings when in captivity to maximise its
wellbeing, has been increasingly applied to fish
species, particularly those used commercially.
Laboratory species could also benefit from enrichment,
but it is not always clear what constitutes an enriched
environment. The zebrafish, \bioname{Danio rerio}, is
used widely in research and is one of the most
commonly-used laboratory animals. We investigated
whether changing the structural complexity of housing
tanks altered the behaviour of one strain of zebrafish.
Fish were kept in three treatments: (1) very enhanced
(VE); (2) mildly enhanced (ME); and (3) control (CT).
Level of aggression, fertilisation success, and growth
were measured at regular intervals in a subset of fish
in each treatment group. The VE fish were more
aggressive over time than either ME or CT fish, both in
the number of attacks they made against a mirror image
and in their tendency to stay close to their reflection
rather than avoid it. Furthermore, VE fish were shorter
than CT fish by the end of the experiment, though mass
was not significantly affected. There was no
significant effect of treatment on fertilisation
success. These findings suggest that the way in which
fish are housed in the laboratory can significantly
affect their behaviour, and potentially, their growth.
The zebrafish is a shoaling species with a dominance
hierarchy, and so may become territorial over objects
placed in the tank. The enrichment of laboratory tanks
should consider aspects of the species' behaviour.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cortay:2019:DAP,
author = "Aurore Cortay and Tatiana Colchen and Pascal Fontaine
and Alain Pasquet",
title = "Does Addition of Perch Larvae as Prey Affect the
Growth, Development and Cannibalism Rate of Pikeperch
Larvae?",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "21",
day = "18",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010021",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/21",
abstract = "Cannibalism occurs in many cultured fish species,
especially at the larval and juvenile stages of
piscivorous taxa. In farmed percid species, such as
pikeperch (\bioname{Sander lucioperca}), intra-cohort
cannibalism is a major issue inducing significant
losses of the initial stocking density during the first
weeks of rearing. Therefore, the present study was
designed to investigate the effects of perch larvae
(\bioname{Perca fluviatilis}) as live prey on growth,
survival and cannibalism in pikeperch larvae under
experimental conditions. Additionally, zootechnical and
behavioural variables linked to aggressiveness (S
postures, attacks, bites and ingestion), and group
structures were considered. The survival rate was not
different between the two groups (72\% with prey vs.
69\% without prey), but the cannibalism rate was higher
in the group with the prey (28\% vs. 10\%). The means
of final weight and length of pikeperch larvae were
higher in the group fed with perch larvae, but size
heterogeneity measured by the coefficients of variation
for these two parameters did not differ. The specific
growth rate was higher in the group fed with perch
larvae, but there was no difference between the two
groups concerning Fulton's condition factor. Among all
the behavioural variables (aggressiveness, group
structure), none differed between the two groups.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ord:2019:ISP,
author = "James Ord",
title = "Ionic Stress Prompts Premature Hatching of Zebrafish
(\bioname{Danio rerio}) Embryos",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "20",
day = "13",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010020",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/20",
abstract = "Ionic homeostasis is an essential component of
functioning cells, and ionic stress imposed by
excessive salinity can disrupt cellular and
physiological processes. Therefore, increasing salinity
of aquatic environments---a consequence of global
climate change---has the potential to adversely affect
the health of aquatic animals and their ecosystems. The
ability to respond adaptively to adverse environmental
changes is essential for the survival of species, but
animals in early embryonic stages may be particularly
vulnerable, as they cannot easily escape from such
conditions. Herein, the effects of increasing salinity
on the mortality and hatching time of zebrafish
(\bioname{Danio rerio}) embryos were investigated.
Increasing salinity significantly affected mortality
after 24 h of exposure beginning from {$<$2} h
post-fertilisation, with 10 parts per thousand (ppt)
inducing complete lethality. The 24-h LC50 of NaCl to
embryos was estimated to be approximately 5.6 ppt.
Interestingly, 5 ppt, a test concentration only
slightly lower than the LC50, induced earlier hatching
than at lower concentrations. This earlier hatching was
also observed even when exposure was commenced at later
stages of embryogenesis, despite later-stage embryos
not suffering appreciable mortality in response to
salinity. The results suggest that earlier hatching is
a plastic response which may function to enable embryos
to evade unfavourable conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Simat:2019:VAS,
author = "Vida {\v{S}}imat and {\v{Z}}eljka Trumbi{\'c}",
title = "Viability of \bioname{Anisakis} spp. Larvae After
Direct Exposure to Different Processing Media and
Non-Thermal Processing in Anchovy Fillets",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "19",
day = "13",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010019",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/19",
abstract = "Anisakiasis is fish-borne zoonoses caused by nematodes
of the genus Anisakis, contracted by the ingestion of
live L3 infective larvae through consumption of raw,
undercooked or thermally unprocessed seafood products,
such as carpaccio, and white marinated and dry-salted
anchovies. In order to maintain the organoleptic
properties of the product, the freezing of fish prior
to processing is often ignored, especially in
households, and traditional processing methods are not
sufficient to kill Anisakis larvae. In this study, we
investigated the survival and resistance of Anisakis
spp. larvae in different processing solutions including
varying salt and sugar content, lemon juice, acetic
acid, alcohol, wine, and apple vinegar. We also
performed a viability test of larvae during processing
in anchovy fillets. When exposed directly to different
NaCl concentrations, larvae were killed after
approximately three days in the strongest (35\%) and 10
days in the weakest solution (5\%). In lemon juice and
lemon juice with added acetic acid, the survival of
larvae was around 5 days. In intact alcohol vinegar,
larvae were killed under less than 48 h, while in the
solution with water their resistance was prolonged to
almost 40 days. In fillets, larvae showed increased
resistance during carpaccio and white wine vinegar
marinades and only dry salting was effective in
destroying Anisakis spp. larvae.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Coccia:2019:CSE,
author = "Elena Coccia and Francesco Siano and Maria Grazia
Volpe and Ettore Varricchio and Orhan Tufan Eroldogan
and Marina Paolucci",
title = "Chestnut Shell Extract Modulates Immune Parameters in
the Rainbow Trout \bioname{Oncorhynchus mykiss}",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "18",
day = "12",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/18",
abstract = "In this study, chestnut (Castanea sativa) shell was
extracted with different solvents, and immunomodulatory
activity was investigated in an in vitro model system
using blood and intestinal leukocytes of
\bioname{Oncorhynchus mykiss}. Gallic acid (GA) was
used as a standard. Chestnut shell extract (CSE) and GA
readily entered both blood and intestinal leukocytes.
Superoxide anion production and phagocytosis were
decreased by low doses of CSE and increased with high
doses. CSE and GA differently regulated cytokine
expression in blood and intestinal leukocytes. High
doses of CSE upregulated IL-1{\beta}, TNF-{\alpha}, and
IL-10 in intestinal leukocytes and IL-10 in blood
leukocytes. Low doses of CSE upregulated IL-1{\beta}
and TNF-{\alpha} in blood leukocytes. GA appeared to be
effective only in blood leukocytes. The effects of CSE
on pro- and anti-inflammatory cytokines seemed to
suggest an alert effect of the immune defense system
against a possible infectious agent. The less evident
effect of GA in comparison to CSE could have been
attributable to the synergistic and/or additive effects
of polyphenols in the latter. The immune-stimulating
activity of CSE reported here could be useful for
future practical applications in fish health.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Unger:2019:RST,
author = "Shem Unger and Caleb Hickman",
title = "Report on the Short-Term Scavenging of Decomposing
Native and Non-Native Trout in {Appalachian} Streams",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "17",
day = "01",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010017",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/17",
abstract = "Trout fishing is one of the primary recreational
activities in the southern Appalachians, with large
amounts of fish stocked on a regular basis. However,
very little is known regarding the fate of hatchery
reared carcasses not captured by anglers, representing
a likely important ecological resource to local
communities. We tested the efficacy of underwater video
to characterize short term decomposition and
consumption by aquatic scavengers of native brook and
non-native rainbow trout, \bioname{Salvelinus
fontinalis} and Oncorhychus mykiss. This study took
place on the Cherokee Qualla Boundary in North
Carolina, a location with one of the highest riverine
stocked trout densities in the eastern {United States}.
During May 2017, 10 waterproof cameras were deployed
for 1-hour intervals on each carcass twice daily for a
period of 5 days. We observed that 75.3\% of recorded
video contained river chub, Nocomis micropogon, with
only 24.7\% visited by crayfish, with a maximum of 9
and a mean of 1.93 for N. micropogon. Half of the
carcasses were removed within 2 days. Based on natural
history evidence and some trail cameras, we believe
that otters were removing carcasses. Otters showed no
preference for either trout species. Underwater video
allowed us to characterize initial decomposition within
stream diurnal scavengers in a short period using a
visual, non-destructive low-cost method. Future studies
should monitor large mammalian scavengers to further
elucidate the role of fish stocking on aquatic
communities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wylie:2019:TCD,
author = "Matthew J. Wylie and Jane E. Symonds and Alvin N.
Setiawan and Glen W. Irvine and Hui Liu and Abigail
Elizur and P. Mark Lokman",
title = "Transcriptomic Changes during Previtellogenic and
Vitellogenic Stages of Ovarian Development in Wreckfish
({H{\=a}puku}), \bioname{Polyprion oxygeneios}
({Perciformes})",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "16",
day = "28",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/16",
abstract = "`Wreckfish' a collective of species belonging to the
family Polyprionidae, are an important commercial
fishery and have significant aquaculture potential.
Until now, genomic or transcriptomic information for
any species within the genus Polyprion has either
remained unpublished or is non-existent. Using Illumina
HiSeq, we compared the transcriptomes of h{\=a}puku
(\bioname{Polyprion oxygeneios}) ovaries to explore
developmental stage-specific variations underlying
their reproductive physiology. We sought to identify
differentially expressed genes and the associated
shifts in biological pathways between previtellogenic
and early vitellogenic ovaries. Ovarian tissue was
repeatedly biopsied by gonopore cannulation from the
same females (n = 3) throughout oogenesis. Reproductive
status of initial biopsies was confirmed as being
previtellogenic and that in biopsies collected eight
weeks later as early vitellogenic. A de novo h{\=a}puku
transcriptome was assembled (146,189 transcripts) from
RNA-Seq data without a reference genome. On average,
each tissue sample contained 17.5 million trimmed
reads. Gene annotation was 80\% when using BLASTX
against Genbank Non Redundant database. Fifty-three
transcripts were differentially expressed within the
FDR of 0.05 when previtellogenic and early vitellogenic
ovaries were compared; this reduced to 35
differentially expressed genes when transcript
duplications were pooled. Among these were genes
tentatively associated with the electron transport
chain, lipid metabolism, steroidogenesis and
mineral/solute transportation. These data provide a
snap-shot into stage-specific physiological events
during oogenesis in the ovary of a teleost and an
extensive molecular resource for research on species in
the Genus Polyprion.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Torrecillas:2019:SMF,
author = "Silvia Torrecillas and Daniel Montero and David
Dom{\'\i}nguez and Lidia Robaina and Marisol
Izquierdo",
title = "Skin Mucus Fatty Acid Composition of Gilthead Sea
Bream (\bioname{Sparus aurata}): a Descriptive Study in
Fish Fed Low and High Fish Meal Diets",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "15",
day = "27",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010015",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/15",
abstract = "Terrestrial protein and lipid sources are commonly
used as substitutes for marine fishery-derived raw
ingredients in fish diets. However, their use is
related with several side-effects on marine fish
performance, health, or disease resistance. Physical
barriers of the skin, gills, and gut constitute the
primary defense mechanism of fish. Skin mucus mucosal
mucins, water, proteins, ions, and lipids determine the
physical, chemical, and protective characteristics of
skin mucus. Very little is known about the influence of
diet composition on fish skin mucus fatty acid profile.
Gilthead sea bream skin mucus contained 10\% of total
lipids (TL), which consisted of 50--60\% neutral (NL)
and 40--50\% polar lipids (PL) fractions. {\Sigma}n{-}3
long chain polyunsaturated fatty acids (LC-PUFA)
deposition was preferential in the NL fraction, whereas
{\Sigma}n{-}6LC-PUFA accumulation was similar in both
lipid classes. Docosahexaenoic acid (DHA; 22:{6n-3})
was the main LC-PUFA stored in skin mucus (14\% TL) in
relation to eicosapentaenoic acid (EPA; 20:{5n-3})
(2--3\% TL) and arachidonic acid (ARA; 20:{4n-6}) (2\%
TL). This study denotes the importance of DHA as
component of skin mucus lipids compared to other
essential fatty acids, such as EPA and ARA, as well as
importance of maintaining an adequate {\Sigma}n{-}3/
{\Sigma}n{-}6 ratio, regardless of dietary intake.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Perez:2019:WPA,
author = "Evaristo P{\'e}rez and F{\'a}tima Linares and Jos{\'e}
Luis Rodr{\'\i}guez Villanueva and Antonio Vilar and
Constantinos C. Mylonas and Ioannis Fakriadis and Maria
Papadaki and Nikos Papandroulakis and Ioannis Papadakis
and Roc{\'\i}o Robles and Christian Fauvel and Javier
Roo and Jos{\'e} Benito Peleteiro and Nuria Lluch and
Gema Pazos and Bel{\'e}n M{\'e}ndez and Irini Sigelaki
and Castora G{\'o}mez and Montse P{\'e}rez and Blanca
{\'A}lvarez-Bl{\'a}zquez",
title = "Wreckfish (\bioname{Polyprion americanus}). New
Knowledge About Reproduction, Larval Husbandry, and
Nutrition. {Promise} as a New Species for Aquaculture",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "14",
day = "25",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010014",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/14",
abstract = "Four different wreckfish (\bioname{Polyprion
americanus}) broodstock batches were maintained in
research facilities under different photo and
thermo-period conditions, one in Greece, the Helenic
Center for Marine Research (HCMR, n = 3) and three in
Spain: Instituto Espa{\~n}ol de Oceanograf{\'\i}a (IEO,
n = 13) in Vigo, Aquarium Finisterrae (MC2, n = 21) in
A Coru{\~n}a and Conseller{\'\i}a do Mar (CMRM, n =
11). The CMRM includes two centers that work together:
Instituto Galego de Formaci{\'o}n en Acuicultura
(IGAFA) and Centro de Investigaci{\'o}ns Mari{\~n}as
(CIMA), both in Pontevedra. During the five years of
the project DIVERSIFY (Exploring the biological and
socio-economic potential of new-emerging candidate fish
species for the expansion of the European aquaculture
industry, 2013--2018) works focused on the reproductive
biology of the species, broodstock, and larvae
nutrition and development of incubation and larval
rearing protocols have been carried out. In terms of
reproduction, catch methods of new wild animals, the
reproductive cycle, sperm characteristics evaluation,
and spontaneous and induced spawning methods have been
described for wreckfish. Regarding nutrition, the
positive effect of two types of enrichment on the fatty
acid profiles of Artemia and rotifer has been verified.
The relationship between the fatty acid profile of the
diets supplied to the broodstock and the fatty acid
profile obtained in the oocytes and eggs of the females
fed with different diets, has also been demonstrated.
Finally, early larval ontogeny has been described and
incubation and larval rearing protocols have been
proposed based on the results obtained in the different
experiments of temperature, growth, survival, and
larval feeding that were carried out.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Debenedetti:2019:PRA,
author = "{\'A}ngela L. Debenedetti and Elena Madrid and
Mar{\'\i}a Trelis and Francisco J. Codes and Florimar
Gil-G{\'o}mez and Sandra S{\'a}ez-Dur{\'a}n and
M{\`a}rius V. Fuentes",
title = "Prevalence and Risk of Anisakid Larvae in Fresh Fish
Frequently Consumed in \geoname{Spain}: an Overview",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "13",
day = "21",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010013",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/13",
abstract = "Anisakidosis is a fish-borne zoonosis caused by
parasitic nematodes of the family Anisakidae, of which
the species belonging to \bioname{Anisakis simplex}
complex are the most representative. It is considered
an emerging disease in Spain. The objective of this
study is to analyse the presence of larvae in fish
frequently consumed in Spanish supermarkets, inferring
the risk of infection. In total 1,786 specimens of 9
different fish species, from two geographical origins
(Atlantic and Mediterranean), acquired fresh and not
eviscerated were examined for anisakid nematodes.
Analysis showed that 33.7\% of the samples were
parasitized by Anisakis larvae. The horse mackerel
(\bioname{Trachurus trachurus}) presented the highest
total prevalence (66.0\%), followed by the silver hake
(\bioname{Merluccius bilinearis}) (59.5\%), the
mackerel (\bioname{Scomber scombrus}) (58.4\%), the
blue whiting (\bioname{Micromesistius poutassou})
(53.9\%) and the European hake (Merlucius merlucius)
(45.0\%). In general, the prevalence was higher in
Atlantic than in Mediterranean fish. In all the species
analysed, a higher presence of the parasite was
detected in the viscera than in the flesh, although in
the most parasitized species a noteworthy prevalence
and abundance was observed in the flesh. In conclusion,
risk factors, like fish species and origin, should be
considered by consumers, in addition of following the
recommendations established by Commission Regulation
(EU) No1276/2011 and the Spanish Royal Decree
1420/2006.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nijssen:2019:DIS,
author = "Edwin J. Nijssen and Machiel J. Reinders and
Athanasios Krystallis and Gemma Tacken",
title = "Developing an Internationalization Strategy Using
Diffusion Modeling: the Case of Greater Amberjack",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "12",
day = "16",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010012",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/12",
abstract = "For farmers of new fish species, market adoption is
needed in order to grow a viable business. Farmers may
try to sell the new species in their firms' domestic
markets, but they might also look at other markets.
However, as markets are becoming more global and
competitors more international, considering
internationalization may be a necessity rather than a
choice. Using diffusion modelling, and based on results
of an online supermarket experiment, the innovation and
imitation parameters are estimated and diffusion curves
for five countries predicted in an attempt to determine
the best lead market for introducing fillets of farmed
greater amberjack (\bioname{Seriola dumerili}). The
production capacity consequences of implementing
different internationalization strategies (i.e.
``sprinkler'' and ``waterfall'') were also explored. A
waterfall strategy refers to the sequential
introduction of a product in different markets, whereas
the sprinkler strategy concerns the simultaneous
introduction of a product in multiple international
markets. Since a sprinkler approach requires many
resources and the ability to quickly ramp up production
capacity, a waterfall approach appears more suitable
for farmers of greater amberjack. Italy and Spain
appear to be the best lead markets for greater
amberjack farmers to enter first.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tarasco:2019:AOA,
author = "Marco Tarasco and Jo{\~a}o Cardeira and Michael N.
Viegas and Joana Caria and Gil Martins and Paulo J.
Gavaia and M. Leonor Cancela and Vincent Laiz{\'e}",
title = "Anti-Osteogenic Activity of Cadmium in Zebrafish",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "11",
day = "15",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010011",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/11",
abstract = "Among the many anthropogenic chemicals that end up in
the aquatic ecosystem, heavy metals, in particular
cadmium, are hazardous compounds that have been shown
to affect developmental, reproductive, hepatic,
hematological, and immunological functions in teleost
fish. There is also evidence that cadmium disturbs bone
formation and skeletal development, but data is scarce.
In this work, zebrafish was used to further
characterize the anti-osteogenic/osteotoxic effects of
cadmium and gain insights into underlying mechanisms.
Upon exposure to cadmium, a reduction of the opercular
bone growth was observed in 6-days post-fertilization
(dpf) larvae and an increase in the incidence of
skeletal deformities was evidenced in 20-dpf
post-larvae. The extent and stiffness of newly formed
bone was also affected in adult zebrafish exposed to
cadmium while regenerating their caudal fin. A pathway
reporter assay revealed a possible role of the MTF-1
and cAMP/PKA signaling pathways in mechanisms of
cadmium osteotoxicity, while the expression of genes
involved in osteoblast differentiation and matrix
production was strongly reduced in cadmium-exposed
post-larvae. This work not only confirmed cadmium
anti-osteogenic activity and identified targeted
pathways and genes, but it also suggested that cadmium
may affect biomechanical properties of bone.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Novianto:2019:DAI,
author = "Dian Novianto and Ilham and Chandara Nainggolan and
Syarif Syamsuddin and Arief Efendi and Sugianto Halim
and Yaser Krisnafi and Muhamad Handri and Abdul Basith
and Yusrizal and Erick Nugraha and Suciadi Catur
Nugroho and Bram Setyadji",
title = "Developing an Abundance Index of Skipjack Tuna
(\bioname{Katsuwonus pelamis}) from a Coastal Drifting
Gillnet Fishery in the Southern Waters of {Indonesia}",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "10",
day = "11",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010010",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/10",
abstract = "Skipjack tuna is targeted by various types of fishing
gear in coastal countries. Due to its resilience, it
has withstood heavy fishing pressure in the past few
decades. Coastal drifting gillnet fleets also mark
skipjack as their main target, but it is often
overlooked in terms of stock assessment. This study
provides new information on an abundance index based on
fishery-dependent data from 2010 to 2017. Generalized
linear models (GLMs) were used to standardize the
catch-per-unit-of-effort (CPUE) using year, quarter,
and gross tonnage as the prediction variables. Model
goodness-of-fit and model selection were based on the
Akaike Information Criterion (AIC), the pseudo
coefficient of determination (R 2), and model
diagnostics with a residual analysis. The final
estimation of the abundance index was calculated by
least square means or marginal means. The results
showed that the index was heavily influenced by the
year and quarter, but it did not relate to the vessel's
capacity. While the CPUE series fluctuated greatly, it
showed a declining trend over the years of
observation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shamsi:2019:SBP,
author = "Shokoofeh Shamsi",
title = "Seafood-Borne Parasitic Diseases: a {``One-Health''}
Approach Is Needed",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "9",
day = "09",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010009",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/9",
abstract = "Global consumption of seafood is steadily increasing,
as is the variety of seafood, including dishes with raw
or undercooked fish, leading to an increased risk of
seafood-borne parasitic diseases. To address today's
challenges to understand the biology and ecology of
these parasites in an ever-changing environment and to
tackle their pathogenicity, multidisciplinary research
is needed. In addition, the gap between research and
stakeholders must be bridged to decrease the risk these
parasites pose to public health. A ``One-Health''
approach to research is necessary to ensure that
consumers, aquatic animals, and environmental health
questions are assessed in an integrated and holistic
manner, resulting in a more comprehensive understanding
of the issues associated with seafood-borne parasitic
diseases and potential solutions. However, when it
comes to seafood-borne parasitic diseases, there is
limited guidance available for a ``One-Health''
approach since these diseases can be less known. In
this article, the focus is on parasitic diseases caused
by seafood, which have been less studied even in some
developed countries where seafood is popular. A brief
overview of some of the seafood-borne parasitic
diseases is provided followed by the significance of
the awareness among various stakeholders in a country.
In this article, it is argued that researchers and
stakeholders are closely connected and a knowledge gap
in one can result in a gap in knowledge and awareness
in the other, causing an inability to accurately
estimate the issues caused by these parasites. It is
suggested that raising awareness, supporting research
and training of all stakeholders are crucial for the
prevention of seafood-borne parasitic diseases and the
protection of the health of seafood consumers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Deakin:2019:WCI,
author = "Anthony G. Deakin and Joseph W. Spencer and Andrew R.
Cossins and Iain S. Young and Lynne U. Sneddon",
title = "Welfare Challenges Influence the Complexity of
Movement: Fractal Analysis of Behaviour in Zebrafish",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "8",
day = "07",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010008",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/8",
abstract = "The ability to assess welfare is an important
refinement that will ensure the good condition of
animals used in experimentation. The present study
investigated the impact of invasive procedures on the
patterns of movement of zebrafish (\bioname{Danio
rerio}). Recordings were made before and after fin
clipping, PIT tagging and a standard pain test and
these were compared with control and sham handled
zebrafish. The fractal dimension (FD) from the 3D
trajectories was calculated to determine the effect of
these treatments on the complexity of movement
patterns. While the FD of zebrafish trajectories did
not differ over time in either the control or sham
group, the FDs of the treatment groups reduced in
complexity. The FD of fish injected with different
strengths of acetic acid declined in a dose-dependent
manner allowing us to develop an arbitrary scale of
severity of the treatments. The 3D trajectory plots
from some groups indicated the presence of repetitive
swimming patterns akin to stereotypical movements. When
administered with lidocaine, which has analgesic
properties, the movement complexity of fin clipped fish
reverted to a pattern that resembled that of control
fish. Fractal analysis of zebrafish locomotion could
potentially be adopted as a tool for fish welfare
assessment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Olsson:2019:PCT,
author = "Jens Olsson",
title = "Past and Current Trends of Coastal Predatory Fish in
the \geoname{Baltic Sea} with a Focus on Perch, Pike,
and Pikeperch",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "7",
day = "06",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010007",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/7",
abstract = "Coastal predatory fish are of key importance for the
provisioning of ecosystem services in the Baltic Sea.
Worldwide, however, there has been a general and sharp
decline in predatory fish populations, in turn
threatening the viability and function of marine
ecosystems. On the basis of the literature, the past
(data until the 2000s) and current (data until early
and mid 2010s) trends in abundance of coastal predatory
fish in the Baltic Sea are reviewed in this paper.
Potentially important impacting factors behind the
temporal development of the populations and measures to
strengthen and restore them are also discussed.
Available data from coastal fish monitoring programs
suggest a stable or increasing abundance of coastal
predatory fish as a functional group and for the
species perch in the majority of areas assessed in the
Baltic Sea. For pike and pikeperch, data to support
assessments is scarce, but suggest substantial declines
in the abundance of both species in most assessed
areas. The impacting factors behind these patterns vary
between species and areas, but include climate, habitat
exploitation, fishing, and species-interactions in the
coastal food web. Measures to restore and support
coastal predatory fish communities should follow an
ecosystem-based approach to management and include
efforts to regulate fisheries sectors in combination
with habitat protection and restoration.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ruiz-Jarabo:2019:ESA,
author = "Ignacio Ruiz-Jarabo and Ana Bel{\'e}n Tinoco and Luis
Vargas-Chacoff and Juan Antonio Martos-Sitcha and Ana
Rodr{\'\i}guez-R{\'u}a and Salvador C{\'a}rdenas and
Juan Miguel Mancera",
title = "Environmental Salinity Affects Growth and Metabolism
in Fingerling Meagre (\bioname{Argyrosomus regius})",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "6",
day = "05",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010006",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/6",
abstract = "The meagre (\bioname{Argyrosomus regius}), a farmed
fish in Mediterranean countries, seasonally migrates
from offshore areas to estuaries for reproduction.
During the first two years of life, the meagre
evidences a certain grade of euryhalinity by staying in
brackish waters close to the shore. The aim of the
present study was to establish if fingerling growth in
brackish water is improved compared to seawater, where
current culture procedures are conducted.
Three-month-old fingerlings were maintained for 45 days
under two different salinity regimens (12 and 39 ppt).
Several growth parameters as well as osmoregulatory and
metabolic variables were assessed. Specific growth rate
and hepatosomatic index values revealed that
fingerlings performed better in brackish waters (12
ppt) compared to 39 ppt. This study contributes to
optimizing meagre rearing conditions, thereby
supporting the role of A. regius in aquaculture
diversification.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Diken:2019:IEP,
author = "G{\"u}rkan Diken and Orhan Demir and Mehmet Naz",
title = "The Inhibitory Effects and Positive Contributions of
Live Foods on Protease Activities of Meagre,
\bioname{Argyrosomus regius} ({Asso} 1801), Larvae In
Vitro Assay",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "5",
day = "04",
month = feb,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010005",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/5",
abstract = "The determination of exogenous enzyme contributions in
live food is important in larval feeding. This study
investigated the potential inhibitory effects and
contributions of live foods used from 3 to 32 days
after hatching (DAH) on protease activities of meagre
(\bioname{Argyrosomus regius}) larvae (ML), using in
vitro techniques. Enriched rotifer (R), Artemia nauplii
(A0), and Artemia metanauplii (A1) were tested. The
highest values of protease activities of ML were
determined to occur at 7 DAH for the years 2013 and
2014. The lowest values were observed at 15 DAH in 2013
and at 20 DAH in 2014. Protease activities of R, A0,
and A1 were 21.76 {\pm} 0.31, 36.00 {\pm} 1.48--29.33
{\pm} 0.93, and 416.44 {\pm} 19.7--403.53 {\pm} 11.85
U/mg protein, respectively (p {$<$} 0.05). The highest
inhibitions of live foods were observed at 7 DAH. This
situation was related to the inadequacy of protease
contributions of live feeds, despite the highest
protease value of larvae at 7 DAH (p {$<$} 0.05). The
positive contributions of A1 live food on the protease
activities of ML were significant (p {$<$} 0.05). The
inhibitory effects and positive contributions of live
foods on the survival and growth rates of ML should be
taken into account for future studies considering the
larvae of ML and other marine fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Office:2019:ARF,
author = "Fishes Editorial Office",
title = "Acknowledgement to Reviewers of {{\booktitle{Fishes}}}
in 2018",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "4",
day = "29",
month = jan,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010004",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/4",
abstract = "The determination of exogenous enzyme contributions in
live food is important in larval feeding. This study
investigated the potential inhibitory effects and
contributions of live foods used from 3 to 32 days
after hatching (DAH) on protease activities of meagre
(\bioname{Argyrosomus regius}) larvae (ML), using in
vitro techniques. Enriched rotifer (R), Artemia nauplii
(A0), and Artemia metanauplii (A1) were tested. The
highest values of protease activities of ML were
determined to occur at 7 DAH for the years 2013 and
2014. The lowest values were observed at 15 DAH in 2013
and at 20 DAH in 2014. Protease activities of R, A0,
and A1 were 21.76 {\pm} 0.31, 36.00 {\pm} 1.48--29.33
{\pm} 0.93, and 416.44 {\pm} 19.7--403.53 {\pm} 11.85
U/mg protein, respectively (p {$<$} 0.05). The highest
inhibitions of live foods were observed at 7 DAH. This
situation was related to the inadequacy of protease
contributions of live feeds, despite the highest
protease value of larvae at 7 DAH (p {$<$} 0.05). The
positive contributions of A1 live food on the protease
activities of ML were significant (p {$<$} 0.05). The
inhibitory effects and positive contributions of live
foods on the survival and growth rates of ML should be
taken into account for future studies considering the
larvae of ML and other marine fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Strauch:2019:EOP,
author = "Sebastian M. Strauch and Judith Bahr and Bj{\"o}rn
Ba{\ss}mann and Adrian A. Bischoff and Michael Oster
and Berit Wasenitz and Harry W. Palm",
title = "Effects of Ortho-Phosphate on Growth Performance,
Welfare and Product Quality of Juvenile
\geoname{African} Catfish (\bioname{Clarias
gariepinus})",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "3",
day = "23",
month = jan,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010003",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/3",
abstract = "Ortho-phosphate inside recirculation aquaculture
systems is limited as a consequence of precipitation
and regular water exchange rates. To improve plant
growth in coupled aquaponics, phosphate fertilizer
addition to hydroponics can increase PO 4 {3-} -P
concentrations inside the process water. We
investigated the effects of four PO 4 {3-} -P
concentrations ({$<$10} (P0), 40, 80, 120 mg L {-1}) in
rearing water on growth performance, feed efficiency,
and welfare traits of juvenile African catfish
(\bioname{Clarias gariepinus} Burchell, 1822). By
trend, optimum specific growth rate of 2.66\% d {-1}
and feed conversion ratio of 0.71 were observed at 40
and 80 mg L {-1} PO 4 {3-} -P. Higher PO 4 {3-} -P
significantly affected skin coloration, swimming
activity and external injuries, with the palest and
inactive fish combined with most external injuries in
the P120 group. Mineral and protein contents in the
fish remained unaffected, while fat content inside the
fillets enriched with increasing PO 4 {3-} -P.
Inorganic P in blood plasma increased significantly,
while phosphate concentrations inside the fillet
remained unchanged. We suggest that PO 4 {3-} -P
concentrations of 40 to 80 mg L {-1} do not reduce the
performance of African catfish aquaculture, while
increased values of 120 mg L {-1} affect fish welfare.
This allows limited addition of PO 4 {3-} -P fertilizer
in coupled aquaponics with African catfish to support
plant growth.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cabillon:2019:MBF,
author = "Nikko Alvin R. Cabillon and Carlo C. Lazado",
title = "Mucosal Barrier Functions of Fish under Changing
Environmental Conditions",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "2",
day = "10",
month = jan,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010002",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/2",
abstract = "The skin, gills, and gut are the most extensively
studied mucosal organs in fish. These mucosal
structures provide the intimate interface between the
internal and external milieus and serve as the
indispensable first line of defense. They have highly
diverse physiological functions. Their role in defense
can be highlighted in three shared similarities: their
microanatomical structures that serve as the physical
barrier and hold the immune cells and the effector
molecules; the mucus layer, also a physical barrier,
contains an array of potent bioactive molecules; and
the resident microbiota. Mucosal surfaces are
responsive and plastic to the different changes in the
aquatic environment. The direct interaction of the
mucosa with the environment offers some important
information on both the physiological status of the
host and the conditions of the aquatic environment.
Increasing attention has been directed to these
features in the last year, particularly on how to
improve the overall health of the fish through
manipulation of mucosal functions and on how the
changes in the mucosa, in response to varying
environmental factors, can be harnessed to improve
husbandry. In this short review, we highlight the
current knowledge on how mucosal surfaces respond to
various environmental factors relevant to aquaculture
and how they may be exploited in fostering sustainable
fish farming practices, especially in controlled
aquaculture environments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Oliveira:2019:INN,
author = "Diogo Oliveira and Andr{\'e} M. Machado and Tiago
Cardoso and M{\'o}nica Lopes-Marques and L. Filipe C.
Castro and Raquel Ruivo",
title = "Identification of a Novel Nucleobase-Ascorbate
Transporter Family Member in Fish and Amphibians",
journal = j-FISHES,
volume = "4",
number = "1",
pages = "1",
day = "01",
month = jan,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:22 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/1/1",
abstract = "Nucleobase-Ascorbate Transporter (NAT) family includes
ascorbic acid, nucleobases, and uric acid transporters:
With broad evolutionary distribution. In vertebrates,
four members have been previously recognized, the
ascorbate transporters Slc23a1 and Slc3a2, the
nucleobase transporter Slc23a4 and an orphan
transporter Slc23a3. Using phylogenetic and synteny
analysis, we identify a fifth member of the vertebrate
slc23 complement (slc23a5), present in neopterygians
(gars and teleosts) and amphibians, and clarify the
evolutionary relationships between the novel gene and
known slc23 genes. Further comparative analysis puts
forward uric acid as the preferred substrate for
Slc23a5. Gene expression quantification, using
available transcriptomic data, suggests kidney and
testis as major expression sites in Xenopus tropicalis
(western clawed frog) and \bioname{Danio rerio}
(zebrafish). Additional expression in brain was
detected in D. rerio, while in the Neoteleostei
\bioname{Oryzias latipes} (medaka) slc23a5 expression
is restricted to the brain. The biological relevance of
the retention of an extra transporter in fish and
amphibians is discussed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Uribe:2019:SAO,
author = "Mari Carmen Uribe and Gabino {De la Rosa Cruz} and
Adriana {Garc{\'\i}a Alarc{\'o}n} and Juan Carlos
{Campuzano Caballero} and Mar{\'\i}a Guadalupe
{Guzm{\'a}n B{\'a}rcenas}",
title = "Structures Associated with Oogenesis and Embryonic
Development during Intraovarian Gestation in Viviparous
Teleosts ({Poeciliidae})",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "35",
day = "19",
month = jun,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020035",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/35",
abstract = "Viviparity in teleosts involves, invariably, the ovary
in a gestational role. This type of viviparity is due
to the combination of unique aspects, different from
those found in the rest of vertebrates. These aspects
are: the ovary has a saccular structure; the germinal
epithelium lines the ovarian lumen; the absence of
oviducts; and the intraovarian insemination,
fertilization, and gestation. The communication of the
germinal zone of the ovary to the exterior is via the
caudal zone of the ovary---the gonoduct. The germinal
epithelium is composed of oogonia and oocytes scattered
individually or in cell nests among somatic epithelial
cells. In the ovarian stroma the follicles are included
which are formed by the oocyte, which is surrounded by
follicular cells and the vascularized theca. The
oogenesis comprises three stages: chromatin-nucleolus,
previtellogenesis, and vitellogenesis. There is no
ovulation, as the oocyte is retained in the follicle.
During the insemination, the spermatozoa enter into the
ovarian lumen and the intrafollicular fertilization
occurs, followed by intrafollicular gestation. The
intraovarian gestation of poeciliids involves
morphological characteristics associated with the
intrafollicular embryogenesis and types of nutrition,
such as lecithotrophy and matrotrophy. In
lecithotrophy, the nutrients come from the yolk
reserves stored during oogenesis, whereas in
matrotrophy the nutrients are provided by supplies from
maternal tissues to the embryo during gestation. The
maternal--embryonic metabolic interchanges converge
through the development of the association of maternal
and embryonic blood vessels, establishing a follicular
placenta.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Reinhardt:2019:THC,
author = "Ulrich G. Reinhardt and Peter J. Hrodey",
title = "Trap Happiness and Catch Bias in Sea Lamprey Traps",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "34",
day = "12",
month = jun,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020034",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/34",
abstract = "Sampling fish by trapping can lead to biased
conclusions about a population. We used catch data to
assess differences between two types of traps for adult
sea lamprey (\bioname{Petromyzon marinus}),
submerged-funnel traps and studded-tile traps, which
are angled ramps with trickle flow leading out of the
water. The studded-tile trap at one river caught about
50\% more females than the funnel trap. It caught males
that had a smaller body size and females with a lower
gonado-somatic index (GSI). The likelihood of catching
lamprey in the studded-tile trap increased after they
had been caught once. This was not the case for the
funnel traps, which are used for mark--recapture-based
population assessment of invasive sea lamprey in the
Great Lakes. The apparent trap response caused by
studded-tile traps may have been caused by a behavioral
bias, i.e., the traps consistently attracting a subset
of the population. Use of the studded-tile trap for
population assessment should only be considered after
more is known about its recapture bias. The differences
between lamprey caught in the two trap types suggests
that a variety of trapping methods needs to be employed
in order to get a representative sample from a fish
population.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Franklin:2019:SKR,
author = "Maxwell Franklin and Martin Cenek and E. Jamie
Trammell",
title = "Studying \geonames{Kenai River} Fisheries'
Social-Ecological Drivers Using a Holistic Fisheries
Agent-Based Model: Implications for Policy and Adaptive
Capacity",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "33",
day = "31",
month = may,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020033",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/33",
abstract = "Alaska's salmon fisheries are one of the more
intensely managed natural resources in the world. The
state's salmon fisheries support recreational,
subsistence, and commercial harvest with multiple
billions of dollars flowing into the economy, and
define the cultural identity of many Alaskans. Fishery
management practices rely on historic records to set
policies with two goals: to meet salmon escapement
quota and to maximize salmon harvest. At the same time,
rapid social and ecological changes to the sub-Arctic
are already impacting salmon runs and fisheries
management. Combined with the inability of fishery
managers to test the outcome of proposed policy
changes, an understanding of the role social and
ecological drivers play in harvest and effort is
required. To address the two-forked problem of
understanding socio-ecological dynamics and potential
policy responses to ecological and social changes, we
(1) conducted stakeholder workshops to solicit key
system drivers, (2) built an integrated agent based
model (ABM) of the system's socio-ecological dynamics,
and (3) tested the impacts of alternative future
scenarios of ecological, social, and policy changes on
the system's outcomes. We previously constructed and
validated a high-fidelity, data-driven, agent-based
model of the Kenai River, Alaska that simulates
seasonal harvest of sockeye and {Chinook} salmon, the
fishing activities of the personal use fishery,
commercial drift, and set gillnet agents. We study the
role of key stakeholder and ecological drivers, using
the ABM decision support tool, and their implications
for fisheries management policies. Analysis of the
scenario based studies found resilience in management
of commercial fisheries to changing salmon migration
dynamics, a lack of adaptive capacity in recreational
(personal use) dipnet users to altered sockeye salmon
runs, and the possible utility of introducing
management measures in the dipnet fishery to manipulate
sockeye escapement levels. These findings represent the
usefulness of this type of ABM in assisting fishery
managers everywhere in investigating possible future
outcomes of different management or ecological
scenarios.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bergsson:2019:RPE,
author = "Hei{\eth}rikur Bergsson and Nikolaj Reducha Andersen
and Morten Bo S{\o}ndergaard Svendsen and Per Juel
Hansen and John Fleng Steffensen",
title = "Respiratory Physiology of \geoname{European} Plaice
(\bioname{Pleuronectes platessa}) Exposed to
\bioname{Prymnesium parvum}",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "32",
day = "28",
month = may,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020032",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/32",
abstract = "During the last century, the blooms of the toxic
haptophyte \bioname{Prymnesium parvum} have been
responsible for massive fish kills in both aquaculture
and wild populations. Despite decades of research, the
ichthyotoxic properties of P. parvum, and how this alga
affects fish, is still debated. Using a novel device to
measure the respirometry, ventilation volume,
ventilation frequency, oxygen extraction, and oxygen
consumption of undisturbed European plaice
(\bioname{Pleuronectes platessa}) were acquired during
exposure to two algal species as well as hypoxia.
Fourteen fish (258 {\pm} 44 g) were initially exposed
to severe hypoxia and left to recover for at least 48
h. Half of these fish were then exposed to known
harmful concentrations of P. parvum (median {\pm}
standard deviation (SD); 2.6 $ \times $ 10 5 {\pm} 0.6
$ \times $ 10 5 cells mL {-1}), while the remaining
half were exposed to the non-toxic alga Rhodomonas
salina (median {\pm} SD; 3.2 $ \times $ 10 5 {\pm} 0.7
$ \times $ 10 5 cells mL {-1}). During exposure to
severe hypoxia, all of the fish were able to maintain
oxygen consumption by increasing the ventilation
volume. The results from fish that were exposed to P.
parvum showed a significant decrease in oxygen
extraction (median {\pm} SD; 52.6 {\pm} 6.9 percentage
points) from pre-exposure to the end of the experiment,
as opposed to fish exposed to R. salina, which were
unaffected. These results indicate that suffocation
affects the European plaice when exposed to P. parvum.
The observed severe decrease in oxygen extraction can
be ascribed to either damage of the gill epithelia or
increased mucus secretion on the gills, as both would
limit the transfer of oxygen, and both have been
observed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fife-Cook:2019:PWF,
author = "Isabel Fife-Cook and Becca Franks",
title = "Positive Welfare for Fishes: Rationale and Areas for
Future Study",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "31",
day = "23",
month = may,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020031",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/31",
abstract = "Traditional animal welfare paradigms have focused on
maintaining physical health and mitigating negative
impacts to wellbeing. Recently, however, the field has
increasingly recognized the importance of positive
welfare (i.e., mental and physical states that exceed
what is necessary for immediate survival) and
accordingly introduced manipulations and indicators of
positive welfare for use in agriculture, laboratories,
and zoos/aquaria. The creation and monitoring of
positive welfare requires an in-depth knowledge of
species-specific behavior and biology, which
necessitates species-specific or, at a minimum,
taxa-specific standards. Research on positive welfare
in fish is lagging in this regard and therefore merits
further consideration. In this paper, we aim to merge
what is already known about positive welfare with the
existing fish behavior literature to develop a plan of
action for fish welfare research that will ultimately
contribute to the development of positive welfare
standards and assessment strategies for fish. We begin
by exploring the origins of positive welfare research
and then outline the physical, psychological and
species-specific areas of inquiry that can be
investigated in fish. In addition to presenting current
findings on fish motivation, emotion, potential sources
of positive welfare such as fulfillment of motivational
urges (establishing agency, engaging in exploration and
learning), and play behavior, we also identify
promising areas for future research aimed at developing
accurate and appropriate indicators of positive welfare
in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Saraiva:2019:GAW,
author = "Jo{\~a}o Luis Saraiva and Pablo Arechavala-Lopez and
Maria Filipa Castanheira and Jenny Volstorf and Billo
Heinzpeter Studer",
title = "A Global Assessment of Welfare in Farmed Fishes: The
{FishEthoBase}",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "30",
day = "16",
month = may,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020030",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/30",
abstract = "Fish welfare is an essential issue that needs to be
tackled by the aquaculture industry. In order to
address it, studies have been limited to a small number
of species and the information is generally scattered.
In order to have a consistent overview of the welfare
of farmed fishes, we present the FishEthoBase, an
open-access database that ultimately aims to provide
information on the welfare of all fish species
currently farmed worldwide. Presently with 41 species,
this database is directed to all stakeholders in the
field and targets not only to bridge the gaps between
them but also to provide scientific information to
improve the welfare of fish. The current text explains
the database and presents an analysis of the welfare
scores of 41 species, suggesting that (i) the general
welfare state of farmed fishes is poor, (ii) there is
some potential for improvement and (iii) this potential
is related to research on species' needs, but (iv)
there are many remaining knowledge gaps and (v) current
fish farming technologies do not seem to fully address
welfare issues. The existence of a framework, such as
the FishEthoBase, is proposed as fundamental to the
design of strategies that improve the welfare of farmed
fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2019:BRJ,
author = "Jaewoo Kim and Caitlyn Bondy and Catherine M. Chandler
and Nicholas E. Mandrak",
title = "Behavioural Response of Juvenile Common Carp
(\bioname{Cyprinus carpio}) and Juvenile Channel
Catfish (\bioname{Ictalurus punctatus}) to Strobe
Light",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "29",
day = "04",
month = may,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020029",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/29",
abstract = "The movement of fish can be regulated by behavioural
manipulation through non-physical barrier systems.
Aquatic invasive species are becoming one of the major
management issues in North America, and threaten native
aquatic ecosystems, including freshwater fish.
Placements of non-physical barriers in waterways can
help disrupt the movement of invasive fish. This study
examined the effect of a strobe-light stimulus on the
avoidance behaviour of two proxy species, juvenile
common carp (\bioname{Cyprinus carpio}) and juvenile
channel catfish (\bioname{Ictalurus punctatus}), in a
controlled laboratory environment. For each species,
three sequential treatments of pre-stimulus,
strobe-light stimulus, and post-stimulus for 30 min
periods were recorded on acclimated groups of 5
juvenile common carp and 5 juvenile channel catfish
using 15 and 13 replicates, respectively. The
distribution of juvenile common carp individuals
throughout the tank did not change significantly with
treatment, nor did cohesive grouping behaviour.
Similarly, there were no significant differences across
experimental treatments in average location/distance of
juvenile channel catfish relative to the strobe light
or degree of cohesion in response to the strobe light.
Non-physical barriers have been widely reported to vary
between species and environmental conditions. These
results suggest that strobe lights evoke no avoidance
or attractive responses in juvenile common carp and
juvenile channel catfish, and will likely not be an
effective barrier to inhibit movements of juvenile
invasive fishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vieira:2019:SVC,
author = "Manuel Vieira and Beatriz P. Pereira and Pedro
Pous{\~a}o-Ferreira and Paulo J. Fonseca and M. Clara
P. Amorim",
title = "Seasonal Variation of Captive Meagre Acoustic
Signalling: a Manual and Automatic Recognition
Approach",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "28",
day = "18",
month = apr,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020028",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/28",
abstract = "Many species rely on acoustic communication to fulfil
several functions such as advertisement and mediation
of social interactions (e.g., agonistic, mating).
Therefore, fish calls can be an important source of
information, e.g., to recognize reproductive periods or
to assess fish welfare, and should be considered a
potential non-intrusive tool in aquaculture management.
Assessing fish acoustic activity, however, often
requires long sound recordings. To analyse these long
recordings automatic methods are invaluable tools to
detect and extract the relevant biological information.
Here we present a study to characterize meagre
(\bioname{Argyrosomus regius}) acoustic activity during
social contexts in captivity using an automatic
pattern-recognition methodology based on the Hidden
Markov Model. Calls produced by meagre during the
breading season showed a richer repertoire than
previously reported. Besides the dense choruses
composed by grunts already known for this species,
meagre emitted successive series of isolated pulses,
audible as `knocks'. Grunts with a variable number of
pulses were also registered. The overall acoustic
activity was concurrent with the number of spawning
events. A diel call rhythms exhibit peak of calling
activity from 15:00 to midnight. In addition, grunt
acoustic parameters varied significantly along the
reproduction season. These results open the possibility
to use the meagre vocal activity to predict breeding
and approaching spawning periods in aquaculture
management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sorensen:2019:BFP,
author = "Peter W. Sorensen and Mara C. P. Rue and Joseph M.
Leese and Ratna Ghosal and Hangkyo Lim",
title = "A Blend of {F} Prostaglandins Functions as an
Attractive Sex Pheromone in Silver Carp",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "27",
day = "09",
month = apr,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020027",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/27",
abstract = "A series of laboratory experiments tested the
hypothesis that the Silver Carp
(\bioname{Hypophthalmichthys molitrix}), an invasive
river carp from China, employs a prostaglandin F
2{\alpha} -derived sex pheromone that is attractive and
species-specific. Using electro-olfactogram recording
(EOG), we found that the olfactory system of this
species is acutely sensitive to three F-series
prostaglandins (PGFs) at picomolar concentrations and
that this sensitivity is enhanced when juveniles are
masculinized using androgens, consistent with
expectations of a sex pheromone. Individual PGFs had
behavioral activity but it was low, suggesting a
mixture might be important. To pursue this possibility,
we implanted carps with osmotic pumps containing
prostaglandin F 2{\alpha} (PGF 2{\alpha}), a treatment
previously shown to elicit release of a PGF-based
spawning pheromone in the Common Carp. We found that
PGF 2{\alpha} -implanted Silver Carp released a
species-specific odor that contained a blend of PGF
2{\alpha} and two of its metabolites, which
masculinized individuals detected and were attracted to
with high sensitivity. Finally, we found that a mixture
of these PGFs was attractive to masculinized Silver
Carp, while a different mixture released by Bighead
Carp was not. We conclude that Silver Carp likely use a
species-specific PGF 2{\alpha} -derived sex pheromone
that is probably released at spawning and might be
useful in its control. Confirmatory studies that
explore pheromone function in naturally mature Silver
Carp using natural odors in the field should now be
conducted to further confirm our proof-of-concept
study.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Moreira:2019:IAS,
author = "M{\'a}rcio Moreira and Ana{\'\i}sa Cordeiro-Silva and
Marisa Barata and Pedro Pous{\~a}o-Ferreira and
Florbela Soares",
title = "Influence of Age on Stress Responses of White Seabream
to Amyloodiniosis",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "26",
day = "08",
month = apr,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020026",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/26",
abstract = "Amyloodiniosis is a disease that represents a major
bottleneck for semi-intensive aquaculture, especially
in Southern Europe. The inefficacy of many of the
treatments for this disease on marine fish produced in
semi-intensive aquaculture has led to a new welfare
approach to amyloodiniosis. There is already some
knowledge of several welfare issues that lead to
amyloodiniosis as well as the stress, physiological,
and immunological responses to the parasite by the
host, but no work is available about the influence of
fish age on the progression of amyloodiniosis. The
objective of this work was to determine if stress,
hematological, and histopathological responses are age
dependent. For that purpose, we determined the
mortality rate, histopathological lesions,
hematological indexes, and stress responses (cortisol,
glucose, lactate, and total protein) in ``Small''
(total weight: 50 {\pm} 5.1 g, age: 273 days after
eclosion (DAE)) and ``Big'' (total weight: 101.3 {\pm}
10.4 g, age: 571 DAE) white seabream (\bioname{Diplodus
sargus}) subjected to an \bioname{Amyloodinium
ocellatum} infestation (8000 dinospores mL {-1}) during
a 24-h period. The results demonstrated a strong stress
response to A. ocellatum, with marked differences in
histopathological alterations, glucose levels, and some
hematological indexes between the fish of the two
treatments. This work elucidates the need to take in
account the size and age of the fish in the development
and establishment of adequate mitigating measures and
treatment protocols for amyloodiniosis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Maslov:2019:CAS,
author = "Dmitry L. Maslov and Oxana P. Trifonova and Anton N.
Mikhailov and Konstantin V. Zolotarev and Kirill V.
Nakhod and Valeriya I. Nakhod and Nataliya F. Belyaeva
and Marina V. Mikhailova and Petr G. Lokhov and
Alexander I. Archakov",
title = "Comparative Analysis of Skeletal Muscle Metabolites of
Fish with Various Rates of Aging",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "25",
day = "31",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020025",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/25",
abstract = "Fish species exhibit great diversity rating of aging
(from negligible to rapid), which gives a unique
possibility for the discovery of the molecular
mechanisms that determine the differences in the rate
of aging. A mass spectrometric metabolic profiling of
skeletal muscle of fish with various aging rates was
carried out by direct injection to a quadrupole
time-of-flight mass spectrometer. The first group
includes long-lived fish species (pike (\bioname{Esox
lucius}) and sterlet (\bioname{Acipenser ruthenus}));
the second group---species with gradual senescence such
as that observed in many mammalian species of similar
size (zander (Sandra lucioperca) and perch
(\bioname{Perca fluviatilis})) and the third
group---species with very short life cycle (chum salmon
(\bioname{Oncorhynchus keta}) and pink salmon
(\bioname{Oncorhynchus gorbuscha})). Multivariate
analysis of metabolic profiles allowed the detecting of
about 80 group-specific features associated with amino
acids, lipids, biogenic amines, intermediates of
glycolysis, glycogenolysis, and citric acid cycle.
Possible roles in the aging process are hypothesized
for the biochemical pathways of the metabolites that
were altered in the different groups.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ido:2019:PMP,
author = "Atsushi Ido and Motohisa Kanemaru and Yoshiharu
Tanioka",
title = "Preliminary Monitoring of Praziquantel in Water and
Sediments at a \geoname{Japanese} Amberjack
(\bioname{Seriola quinqueradiata}) Aquaculture Site",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "24",
day = "28",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020024",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/24",
abstract = "Praziquantel (PZQ), an active compound against
Platyhelminthes, is an essential anthelmintic for the
aquaculture industry. However, there are few reports of
the environmental risks of PZQ use in aquaculture. In
this study, we monitored PZQ in water and sediment at
an aquaculture site of Japanese amberjack, also called
yellowtail (\bioname{Seriola quinqueradiata}). Although
PZQ was detected in water during treatment and 3 days
post-treatment, PZQ levels were below the detectable
limit in water 60 m from the net pen during the
treatment, and in all sediment samples. In this
preliminary study, we could not detect residue of PZQ
from sediments in the aquaculture site, and no evidence
about environmental effect of PZQ administration was
obtained.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Goncalves-de-Freitas:2019:SBW,
author = "Eliane Gon{\c{c}}alves-de-Freitas and Marcela Cesar
Bolognesi and Ana Carolina dos Santos Gauy and Manuela
Lombardi Brand{\~a}o and Percilia Cardoso Giaquinto and
Marisa Fernandes-Castilho",
title = "Social Behavior and Welfare in \geoname{Nile}
Tilapia",
journal = j-FISHES,
volume = "4",
number = "2",
pages = "23",
day = "27",
month = mar,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4020023",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/2/23",
abstract = "Fish social behavior can be affected by artificial
environments, particularly by factors that act upon
species that show aggressive behavior to set social
rank hierarchy. Although aggressive interactions are
part of the natural behavior in fish, if constant and
intense, such interactions can cause severe body
injuries, increase energy expenditure, and lead the
animals to suffer from social stress. The immediate
consequence of these factors is a reduced welfare in
social fish species. In this paper, we consider the
factors that impact on the social behavior and welfare
of Nile tilapia, an African cichlid fish widely used
both in fish farms and in research; this species is
frequently used as a model for physiology and behavior
research. This is a polygynous species whose males
interact aggressively, establishing a territorial based
hierarchy, where a dominant male and several
subordinate males arise. When social stability is
shrunk, the negative effects of prolonged fighting
emerge. In this paper, we summarized how some of the
common practices in aquaculture, such as classifying
individuals by matching their sizes, water renewal,
stock density, and environment lighting affect Nile
tilapia social aggressive interactions and, in turn,
impact on its welfare. We also discuss some ways to
decrease the effects of aggressive interactions in Nile
tilapia, such as environment color and body tactile
stimulation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{vonKrogh:2019:VEB,
author = "Kristine von Krogh and Erik Ropstad and Rasoul
Nourizadeh-Lillabadi and Trude Marie Haug and Finn-Arne
Weltzien",
title = "In Vitro Effects of Bisphenol {A} and
Tetrabromobisphenol {A} on Cell Viability and
Reproduction-Related Gene Expression in Pituitaries
from Sexually Maturing \geoname{Atlantic} Cod
(\bioname{Gadus morhua} {L.})",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "48",
day = "17",
month = sep,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030048",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/48",
abstract = "Bisphenol A (BPA) and tetrabromobisphenol A (TBBPA)
are widely used industrial chemicals, ubiquitously
present in the environment. While BPA is a well-known
endocrine disruptor and able to affect all levels of
the teleost reproductive axis, information regarding
TBBPA on this subject is very limited. Using primary
cultures from Atlantic cod (\bioname{Gadus morhua}),
the present study was aimed at investigating potential
direct effects of acute (72 h) BPA and TBBPA exposure
on cell viability and the expression of
reproductive-relevant genes in the pituitary. The
results revealed that both bisphenols stimulate cell
viability in terms of metabolic activity and membrane
integrity at environmentally relevant concentrations.
BPA had no direct effects on gonadotropin gene
expression, but enhanced the expression of
gonadotropin-releasing hormone (GnRH) receptor 2a, the
main gonadotropin modulator in Atlantic cod. In
contrast, TBBPA increased gonadotropin transcript
levels but had no effect on GnRH receptor mRNA. In
conclusion, both anthropogenic compounds display
endocrine disruptive properties and are able to
directly interfere with gene expression related to
reproductive function in cod pituitary cells at
environmentally relevant concentrations in vitro.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rodriguez-Gonzalez:2019:PML,
author = "Tania Rodr{\'\i}guez-Gonz{\'a}lez and Jes{\'u}s Cerezo
Valverde and Benjam{\'\i}n Garc{\'\i}a Garc{\'\i}a",
title = "Performance of Marine Lecithin Supplemented Feeds for
the Common Octopus (\bioname{Octopus vulgaris})
Ongrowing: Changes in Proximate Composition and Lipid
Classes' Profile",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "47",
day = "12",
month = sep,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030047",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/47",
abstract = "The development of artificial diets is considered
vital for feasible cephalopods' culture.
\bioname{Octopus vulgaris} need a diet with a high
protein content but also lipids are important at a
lower quantity, as polar lipids and LC-PUFA are
essential for development during early stages. In the
present study the suitability of marine lecithin as a
dietary supplement for O. vulgaris juveniles'
formulated feeds was tested for 56 days, assessing the
performance, changes in proximate composition, and
lipid classes' profile in the digestive gland and
carcass. Sixteen octopus were fed one of two semi-moist
feeds based on dry ingredients: either CALPRO (N = 4)
as control or CALPRO-LM (N = 8); which differed from
the first, due to the inclusion of 20 g/kg of marine
lecithin as a phospholipid dietary supplement. Results
showed that marine lecithin did not enhance feed
intake, growth, protein or lipid incorporation,
nutrients digestibility or feed efficiency. Moreover,
at this level of inclusion, the composition of tissues
(digestive gland and carcass) regarding macronutrients
and lipid classes' profile presented only a small
amount of differences. In conclusion, the inclusion of
marine lecithin did not promote beneficial effects on
performance, making necessary further research related
to the nutritional requirements of common octopus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wagner:2019:NBM,
author = "Liane Wagner and Pedro G{\'o}mez-Requeni and Ali A.
Moazzami and Torbj{\"o}rn Lundh and Aleksandar
Vidakovic and Markus Langeland and Anders Kiessling and
Jana Pickova",
title = "{$^1$H} {NMR}-Based Metabolomics and Lipid Analyses
Revealed the Effect of Dietary Replacement of Microbial
Extracts or Mussel Meal with Fish Meal to
\geoname{Arctic} Charr (\bioname{Salvelinus alpinus})",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "46",
day = "10",
month = sep,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030046",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/46",
abstract = "The effects of replacing 40\% of dietary fish meal
(FM) in a reference diet (REF) with either mussel meal
(MM), zygomycete fungi (ZYG), extracted baker's yeast
(EY), or non-extracted baker's yeast (NY) on the lipid
and metabolic profile of Arctic charr
(\bioname{Salvelinus alpinus}) were investigated. After
a 14-week feeding trial, liver and muscle tissues were
collected for lipid (lipid content, lipid class, fatty
acid composition) and 1 H NMR-based metabolomics
analyses (aqueous and chloroform phases). Lipid
analyses showed that fish fed ZYG diet had lower liver
lipid content and thereby 10\% higher level of
docosahexaenoic acid compared with REF. Metabolomics
analyses showed that on the one hand fish fed NY diet
affected liver metabolites (2--3 fold higher
concentrations of e.g., n,n-dimethylglycine and
betaine) compared with REF, while, on the other hand,
the muscle metabolic fingerprint was mainly affected by
EY. In general, affected metabolites (e.g., alanine,
anserine, betaine, hydroxyproline, isoleucine,
malonate, n,n-dimethylglycine, proline, succinate, and
valine) in fish fed test diets suggested that the test
meal ingredients caused mainly a response in muscle
metabolism. Fish metabolism was least affected by MM,
which suggests that it may be suitable to replace fish
meal in Arctic charr diets.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pedescoll:2019:PPW,
author = "Anna Pedescoll and Rafael Aguado and Carlos Marcos and
Gustavo Gonz{\'a}lez",
title = "Performance of a Pool and Weir Fishway for
\geoname{Iberian} Cyprinids Migration: a Case Study",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "45",
day = "16",
month = aug,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030045",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/45",
abstract = "One of the movement barriers that fish populations
must overcome for migration success in the upper basin
of Tormes river (Salamanca, Spain) is a 20 m high dam.
The design of its pool and weir fishway for
potamodromous fishes (mostly Iberian barbel---
Luciobarbus bocagei ---and Northern straight-mouth
nase--- Pseudochondrostoma duriense) to overcome the
obstacle was improved in 2013. The aim of this study
was to assess the efficiency of the fishway using FDX
passive integrated transponder (PIT)-Tags inserted into
the fish and PIT-Tag detection antennas at the fishway.
During several sampling events, 7113 barbel and nase
individuals were tagged and released at the point of
capture along the basin (2538 and 4575 of which were
tagged downstream and upstream, respectively). PIT-Tag
Detection Antennas close to the top and bottom of the
fishway monitored tagged fish continuously for 10
months (from March to December 2017), to analyze the
performance of the fishway. Upstream passage efficiency
was greater for barbel (60\% and 25\% for barbel and
nase, respectively). Differences in passage efficiency
between species may be due to differences in their
size. Mean length for barbels attempting to pass was
336 mm ({\pm}47 mm) while for nases was 143 mm ({\pm}26
mm). Moreover, both the number of attempts to pass and
ascend time for nases were higher than for barbels.
Entrance efficiency was low (3.5\% and 10.8\% for
barbel and nase, respectively), although 2017 was a
very dry year, thus these results are most likely
influenced by flow rates. Therefore, the fishway has
proved to be functional but is actually poor for
efficiency purposes, especially for small fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Burton:2019:PEA,
author = "Michael L. Burton and Jennifer C. Potts and Andrew D.
Ostrowski",
title = "Preliminary Estimates of Age, Growth and Natural
Mortality of Margate, \bioname{Haemulon album}, and
Black Margate, \bioname{Anisotremus surinamensis}, from
the {Southeastern United States}",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "44",
day = "15",
month = aug,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030044",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/44",
abstract = "Ages of margate, Haemulon album (n = 415) and black
margate, Anisotremus surinamensis (n = 130) were
determined using sectioned sagittal otoliths collected
from the Southeastern {United States} Atlantic coast
from 1979 to 2017. Opaque zones were annular, forming
between January and June for both species, with peaks
in occurrence of otoliths with opaque margins in April
for margate and March for black margate. The observed
ages for margate were 0--22 years, and the largest fish
measured 807 mm TL (total length). Black margate ranged
in age from 3 to 17 years, and the largest fish was 641
mm TL. Weight--length relationships were: margate,
ln(W) = 2.88 ln(TL) {-} 10.44 (n = 1327, r 2 = 0.97,
MSE = 0.02), where W is total weight (grams, g); black
margate, ln(W) = 3.02 ln(TL) {-} 11.10 (n = 451, r 2 =
0.95, MSE = 0.01). Von Bertalanffy growth equations
were L t = 731 (1 {-} e {-0}.23(t+0.38)) for margate,
and L t = 544 (1 {-} e {-0}.13(t+2.61)) for black
margate. After re-estimating black margate growth using
a bias-correction procedure to account for the lack of
younger fish, growth was described by the equation L t
= 523 (1 {-} e {-0}.18(t+0.0001)). Age-invariant
estimates of natural mortality were M = 0.19 y {-1} and
M = 0.23 y {-1} for margate and black margate,
respectively, while age-varying estimates of M ranged
from 2.93 {-0}.23 y {-1} for fish aged 0--22 for
margate and 7.20 {-} 0.19 y {-1} for fish aged 0--18
for black margate. This study presents the first
documentation of life-history parameters for margate
from the Atlantic waters off the Southeastern {United
States}, and the first published estimate of black
margate life history parameters from any geographic
region.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Herrera:2019:TSC,
author = "Mercedes Herrera and Raquel Moreno-Valc{\'a}rcel and
Ram{\'o}n De Miguel Rubio and Carlos
Fern{\'a}ndez-Delgado",
title = "From Transient to Sedentary? {Changes} in the Home
Range Size and Environmental Patterns of Movements of
\geoname{European} Eels (\bioname{Anguilla anguilla})
in a \geoname{Mediterranean} River",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "43",
day = "13",
month = aug,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030043",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/43",
abstract = "The habitat use of eels during the development of
sedentary behavior, which depends on the animals' body
size, is unknown. Our objective was to analyze, for two
years, the changes in the home range of a population of
European eel (\bioname{Anguilla anguilla}, Linnaeus,
1758) in Southern Europe in relation to the animals'
body length (TL), and the influence of environmental
factors (water temperature and flow) on the local
movements of this population through observation of
their sedentary behavior. We used a
previously-validated mark--recapture methodology,
obtaining a low deviation in the estimation of the
extension of the eels' movements. Our results revealed
relatively short movements in relation to other
populations, and we hypothesize that this could be
related to the high habitat diversity and low eel
population density in the study area. The home range
size showed a high variability and dispersion among the
smallest eels, however, as TL increased, the
variability of home range size decreased, and home
ranges were larger. These changes could be associated
with the acquisition of a sedentary lifestyle. Once
eels had become sedentary, an environmental pattern was
observed between their movements and the water
temperature and flow, with larger movements observed as
the flow increased and water temperature decreased.
This suggests that the temporal and spatial scales of
observation are of crucial importance for monitoring
eel populations and for the study of population size
structure, population dynamics, and biology, which can
be used to improve conservation strategies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pontigo:2019:MEI,
author = "Juan Pablo Pontigo and Julia Saravia and Ricardo
Oyarz{\'u}n and Margarita P. Gonz{\'a}lez and
Christopher Hawes and Francisco J. Morera and Jorge
Pino and Simon Wadsworth and Jose Luis P. Mu{\~n}oz and
Luis Vargas-Chacoff",
title = "Modulation of the Expression of Immune-related Gene in
\geoname{Atlantic} and {Coho} Salmon during Infestation
with the Sea lice \bioname{Caligus rogercresseyi}",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "42",
day = "25",
month = jul,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030042",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/42",
abstract = "\bioname{Caligus rogercresseyi}, a marine
ectoparasite, causes notable economic losses for the
Chilean salmonid industry. Nevertheless, the
immunological responses of infected fish remain poorly
understood, including proinflammatory cytokine
generation and the respective modulatory effects of
various cytokine receptors. This study evaluated mRNA
expression of the NLRC5, major histocompatibility
complex (MHC) class II, I-kappa-B-alpha, a regulatory
that inhibits NF-kappa-B, and proinflammatory cytokines
(IL-1{\beta} and IL-18) in the liver and muscle of
Atlantic salmon (\bioname{Salmo salar}) and {Coho}
salmon (\bioname{Oncorhynchus kisutch}) during a
time-course C. rogercresseyi infestation trial. All
assessed mRNA were strongly regulated during
infestation, but S. salar showed up-regulated
expression, possibly accounting for the high
infestation vulnerability of this salmonid. In
conclusion, this work helps to understand the
modulation of the expression of different transcripts
involved over short periods of C. rogercresseyi
infestation in two salmonid species (\bioname{S. salar}
and \bioname{O. kisutch}).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wylie:2019:ISF,
author = "Matthew J. Wylie and Alvin N. Setiawan and Glen W.
Irvine and Abigail Elizur and Yonathan Zohar and Jane
E. Symonds and P. Mark Lokman",
title = "Induced Spawning of {F1} Wreckfish ({H{\=a}puku})
\bioname{Polyprion oxygeneios} Using a Synthetic
Agonist of Gonadotropin-Releasing Hormone",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "41",
day = "08",
month = jul,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030041",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/41",
abstract = "Wild-caught h{\=a}puku (\bioname{Polyprion
oxygeneios}) spawn readily in captivity, but although
first filial (F1) h{\=a}puku complete vitellogenesis,
females fail to undergo oocyte maturation and spawn or
produce poor quality eggs. This study investigated
whether administration of a synthetic agonist of
gonadotropin-releasing hormone (GnRHa) could improve F1
h{\=a}puku spawning and complete the life-cycle in
captivity. Spawning trials were conducted over 2 years
in 2013 and 2014, when F1 were aged five and six years.
In 2013, females previously conditioned under a
variable or constant temperature regime were implanted
with GnRHa (100 {\mu}g/kg {-1}) or blank implants
constructed of powdered cellulose and cholesterol.
Spawning was erratic and egg quality very poor in all
tanks. No F2 offspring were produced by communal
spawning. In contrast, viable F2 larvae were produced
by strip-spawning and in vitro fertilization after a
series of GnRHa injections. In 2014, two additional
trials were conducted: females received ethylene-vinyl
acetate copolymer (EVAc) matrix implants containing
GnRHa (100 {\mu}g/kg {-1}) or blank implants and in the
second trial, two GnRHa doses (100 {\mu}g/kg {-1} and
50 {\mu}g/kg {-1}) were tested. Eggs were first
detected in all tanks 12--17 days post-implantation
when females received 100 $ \mu $ g/kg {-1} GnRHa
implants, but not in the lower dose or control tanks.
In summary, this study achieved induction of female
spawning with GnRHa implants (target dose 100 {\mu}g/kg
{-1}) and the successful production of F2 h{\=a}puku in
captivity by strip-spawning.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Taylor:2019:SBC,
author = "Brett M. Taylor",
title = "Standing Out in a Big Crowd: High Cultural and
Economic Value of \bioname{Naso unicornis} in the
Insular \geoname{Pacific}",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "40",
day = "03",
month = jul,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030040",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/40",
abstract = "Hundreds of fish species are harvested in coral-reef
fisheries in the Indo-Pacific region using a variety of
fishing gears. This diversity makes the economics,
ecology, and management of reef-associated fisheries
inherently complex. However, across insular fisheries
spanning the tropical Pacific, one species consistently
dominates contemporary fisheries catch: the bluespine
unicornfish (\bioname{Naso unicornis}, Acanthuridae). I
evaluated the relative contribution of N. unicornis to
commercial fisheries in the insular Pacific region from
long-term ({$>$1} year) fishery surveys across various
jurisdictions and provide evidence of the contemporary
cultural value of this species. Overall, evidence
suggests that N. unicornis is the most
commercially-valuable reef-associated fish species
across the insular Pacific. This notion, coupled with a
diverse representation of N. unicornis across Pacific
cultures, suggests that the species is presently
underappreciated in its role in coastal fisheries
across Pacific Island nations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Saraiva:2019:WFN,
author = "Jo{\~a}o L. Saraiva and Pablo Arechavala-Lopez",
title = "Welfare of Fish --- No Longer the Elephant in the
Room",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "39",
day = "03",
month = jul,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030039",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/39",
abstract = "Hundreds of fish species are harvested in coral-reef
fisheries in the Indo-Pacific region using a variety of
fishing gears. This diversity makes the economics,
ecology, and management of reef-associated fisheries
inherently complex. However, across insular fisheries
spanning the tropical Pacific, one species consistently
dominates contemporary fisheries catch: the bluespine
unicornfish (\bioname{Naso unicornis}, Acanthuridae). I
evaluated the relative contribution of N. unicornis to
commercial fisheries in the insular Pacific region from
long-term ({$>$1} year) fishery surveys across various
jurisdictions and provide evidence of the contemporary
cultural value of this species. Overall, evidence
suggests that N. unicornis is the most
commercially-valuable reef-associated fish species
across the insular Pacific. This notion, coupled with a
diverse representation of N. unicornis across Pacific
cultures, suggests that the species is presently
underappreciated in its role in coastal fisheries
across Pacific Island nations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hashizume:2019:HMD,
author = "Atsushi Hashizume and Atsushi Ido and Takashi Ohta and
Serigne Thierno Thiaw and Ryusaku Morita and Munenori
Nishikawa and Takayuki Takahashi and Chiemi Miura and
Takeshi Miura",
title = "Housefly (\booktitle{Musca domestica}) Larvae
Preparations after Removing the Hydrophobic Fraction
Are Effective Alternatives to Fish Meal in Aquaculture
Feed for Red Seabream (\bioname{Pagrus major})",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "38",
day = "27",
month = jun,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030038",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/38",
abstract = "Insects are an attractive alternative to fish meal
(FM) as a sustainable protein source in aquaculture
feed that does not negatively impact the marine
ecosystem. Despite housefly (Musca domestica) larvae
having adequacy of amino acid profiles, they have
sometimes been reported to be inferior to FM,
especially for marine carnivorous fish species. Here,
we report that the removal of the hydrophobic fractions
from housefly larvae enables significant replacement of
FM in the diet of the red seabream (\bioname{Pagrus
major}). In a feeding trial, housefly (HF) larvae that
had the hydrophobic fraction removed as a complete
substitution for 70\% FM produced satisfactory growth.
However, HF larvae that were supplemented with the
hydrophobic fraction resulted in significant growth
reduction. Growth recovery was incomplete by
supplementation of docosahexaenoic acid (DHA) and
eicosapentaenoic acid (EPA) to undefatted HF larvae,
being equivalent to that of fatty acid content with a
control diet. Moreover, fish with a dietary intake of
catechol identified from the hydrophobic fraction of
the HF showed growth reduction and morphological
alterations in the intestine. Our findings indicate
that the hydrophobic fraction from HF larvae contains a
negative factor for fish growth and eliminating the
fraction from HF larvae is thought to be an important
process for sustainable aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gomez-Requeni:2019:DLC,
author = "Pedro G{\'o}mez-Requeni and Mauricio Nestor Kraemer
and Luis Fabi{\'a}n Canosa",
title = "The Dietary Lipid Content Affects the Tissue Gene
Expression of Muscle Growth Biomarkers and the
{GH\slash IGF} System of Pejerrey (\bioname{Odontesthes
bonariensis}) Juveniles",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "37",
day = "27",
month = jun,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030037",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/37",
abstract = "Gene expression of growth hormone receptors (GHRs),
insulin-like growth factors (IGFs), myostatin (MSTN)
and myogenin (MyoG) was analyzed in juveniles pejerrey
fed with graded levels of lipids (L): 6\% (L6), 10\%
(L10), 25\% (L25). After 14 weeks, no changes were
found in liver GHR-I GHR-II and IGF-II mRNA levels
whereas IGF-I decreased in L10 and L25. Muscle GHR-I
gene expression increased in L25 whereas GHR-II, IGF-II
and MyoG were higher in L6. IGF-I and MSTN expression
was not affected by the different diets. Adipose IGF-I
mRNA levels decreased in L10. Correlations between body
weight and members of GH/IGF system in liver and
skeletal muscle were found only in L10 group.
Correlations found in L10 group between both liver and
skeletal muscle GHR-I and IGF-I were lost in either L6
or L25 groups. Thus, fish fed with apparently
unbalanced dietary lipid contents (6\% and 25\%)
exhibit a compensatory regulation of systemic and local
components of the GH/IGF axis. Furthermore, the marked
inhibition of muscle MyoG gene expression in L25 might
limit excessive lipid deposition and fish growth. Our
data suggest that a dietary lipid contents of 10\%
would promote a particular adjustment of the endocrine
and autocrine/paracrine GH/IGF system, stimulating body
growth and perhaps muscle hyperplasia. On the other
hand, a higher dietary lipid content would uncouple the
GH/IGF system, reducing hepatic IGF-I, while slightly
increasing hepatic GHR-I, probably to prompt
lipolysis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Burton:2019:AGN,
author = "Michael L. Burton and Jennifer C. Potts and Andrew D.
Ostrowski and Kyle W. Shertzer",
title = "Age, Growth, and Natural Mortality of Graysby,
\bioname{Cephalophilis cruentata}, from the
{Southeastern United States}",
journal = j-FISHES,
volume = "4",
number = "3",
pages = "36",
day = "27",
month = jun,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4030036",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:23 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/3/36",
abstract = "Graysby (Cephalophilis cruentata) (n = 1308) collected
from the southeastern {United States} Atlantic coast
from 2001 to 2016 were aged using sectioned sagittal
otoliths. Opaque zones formed February to June (peaking
in April). Ages ranged from 2 to 21 years, and the
largest fish measured 453 mm TL. Growth morph analysis
revealed two regionally distinct growth trajectories:
von Bertalanffy growth equations were L t = 388 (1 {-}
e {-0}.12(t+5.73)) for fish from North Carolina through
southeast Florida (northern region), and L t = 267 (1
{-} e {-0}.17(t+6.20)) for fish from the Florida Keys
(southern region). When growth was re-estimated using a
fixed t 0 value of {-0}.75 to estimate for smaller
fish, growth equations were L t = 349 (1 {-} e
{-0}.26(t+0.75)) and L t = 250 (1 {-} e
{-0}.43(t+0.75)) for fish from the northern and
southern regions, respectively. The age-invariant
estimate of natural mortality was M = 0.30 for all
fish, while age-specific estimates ranged 0.88--0.28 y
{-1} for fish aged 1--21 from the northern region and
0.89--0.47 y {-1} for fish aged 1--15 from the southern
region. This study presents the first comprehensive
analysis of life-history parameters for graysby from
the Atlantic waters off the southeastern {United
States}, including specimens from both recreational and
commercial fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Babin:2019:DRA,
author = "Amanda Babin and Lauren Fitzpatrick and Tommi
Linnansaari and R. Allen Curry",
title = "Detection Range of Acoustic Receivers in a Large
Hydropower Reservoir",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "60",
day = "11",
month = dec,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040060",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/60",
abstract = "Acoustic telemetry manufacturers report estimated
detection ranges under idealized conditions, but
environmental conditions such as water depth, substrate
type, and turbulence can affect the range of reliable
detection. Range testing of low (Vemco V7 136 dB re 1$
\mu $ [email protected]) and high power (V13 147 dB re
1$ \mu $ [email protected]) acoustic transmitters
(tags) was performed near a hydropower generating
station and its associated reservoir using both active
(mobile; VR100) and passive (stationary; VR2W/VR2Tx)
receivers. Low power tags are typically used to track
small fish such as juvenile Atlantic salmon
(\bioname{Salmo salar}), whereas high power tags are
typically used to track larger fish such as adult
salmon. The results found herein were applied to
concurrent salmon telemetry studies. Detection ranges
of the low power tags were within 246--351 {\pm} 20--70
m (mean {\pm} SE), and the high power tags were within
537--1106 {\pm} 53--272 m. Observed detection ranges
were comparable or higher to manufacturer estimates for
both tag types being detected by passive receivers, and
were lower than expected for both tag types being
detected by active receivers. Passive receivers were
further tested by mooring a fixed sentinel tag (low
power) on a receiver line at the hydropower site for 50
days. The sentinel tag detection range of 212 m was
less than the expected range of 280--292 m, and was not
found to be significantly impacted by wind speed. There
was evidence of a hydropower effect on detection
probability (up to 95\% reduction) of both tag types
for the active receiver, and detection ranges were
significantly lower at the hydropower site than the
reservoir site for the high power tag. The results of
this study give insight to the initial design of
acoustic telemetry studies beyond what can be gathered
from manufacturer's estimates, but rather near
hydropower facilities and within large reservoirs;
however, detection ranges reported herein do not
replace the importance of range testing in
site-specific conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Buckwalter:2019:DLD,
author = "Joseph Buckwalter and Paul L. Angermeier and Jane
Argentina and Skylar Wolf and Stephen Floyd and Eric M.
Hallerman",
title = "Drift of Larval Darters (Family {Percidae}) in the
{Upper Roanoke River Basin, USA}, Characterized Using
Phenotypic and {DNA} Barcoding Markers",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "59",
day = "08",
month = dec,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040059",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/59",
abstract = "Larval fish ecology is poorly characterized because
sampling is difficult and tools for phenotypically
identifying larvae are poorly developed. While DNA
barcoding can help address the latter problem,
`universal' primers do not work for all fish species.
The Roanoke River in the southeastern {United States}
includes seven darters (Family Percide: Tribe
Etheostomatini). We made 393 collections of larval
fishes in 2015 and 2018, examined darter larvae for
morphometric and pigmentation traits, developed PCR
primers amplifying darter DNA, and evaluated three gear
types for collecting larval darters. Amplified DNA
sequences for 1351 larvae matched archived
mitochondrial cytochrome oxidase I sequences for
darters occurring in the ecosystem. Larval darters were
classified to genus with 100\% accuracy using the ratio
of pectoral fin length to body length; however,
identification to species using morphometrics alone was
subject to a misclassification rate of 11.8\%, which
can be resolved by considering pigmentation patterns.
Gear-types varied considerably in their capture
efficacy for larval darters; most Percina larvae were
collected in drift nets. Larval Percina species
appeared in the drift before Etheostoma species in both
study years. Application of molecular genetic and
phenotypic tools to larval fish identification can
advance understanding of larval darter ecology.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ferreira:2019:DEC,
author = "Marco Ferreira and Jo{\~a}o Gago and Filipe Ribeiro",
title = "Diet of \geoname{European} Catfish in a Newly Invaded
Region",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "58",
day = "08",
month = dec,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040058",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/58",
abstract = "Biological invasions are considered to be one of the
leading causes of biodiversity loss worldwide.
Freshwater ecosystems are amongst the most biodiverse,
containing about 50\% of the world's known fish species
but, ironically, are the most invaded ecosystems. In
Portuguese freshwaters, there are currently 20
identified non-native fishes, arriving at an
unprecedented rate of a new species every two years. A
recent non-native is the European catfish
\bioname{Silurus glanis}, a top predator native to
Central and Eastern Europe, that arrived at the Lower
Tagus (Portugal) circa 2006. This study compares the
dietary habits of this top predator in two different
habitats of the Lower Tagus in order to evaluate its
potential impact. The stomach contents of 96
individuals were analyzed, 61 from the lotic
environment and 35 from the lentic systems. A total of
66 stomachs contained prey items, 38 from the lotic and
28 from the lentic. Diet varied considerably between
habitats, with shrimp and crayfish being the most
abundant prey items in the lentic systems and shrimp
and fish the most abundant prey items in the lotic
section. Tendency to piscivory was observed in
large-sized individuals. Overall, the findings in this
study provide the first evidence of the potential
impacts of European catfish through predation on the
fish communities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lazado:2019:SPS,
author = "Carlo C. Lazado and Peter Vilhelm Skov",
title = "Secretory Proteins in the Skin Mucus of \geoname{Nile}
Tilapia (\bioname{Oreochromis niloticus}) are Modulated
Temporally by Photoperiod and Bacterial Endotoxin
Cues",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "57",
day = "05",
month = dec,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040057",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/57",
abstract = "Although it is well known that the biological and
physical characteristics of skin mucus in fishes are
strongly affected by changes in environmental
conditions, the influence of photoperiod and
time-dependent bacterial endotoxin stimulation is not
well documented. In the present study, we determined
the diel variations in the basal activities of
secretory proteins with known defense functions in the
skin mucus of Nile tilapia (\bioname{Oreochromis
niloticus}) maintained under two photic environments:
equal length of day and night (12L:12D, LD) or total
darkness (0L:24D, DD). A second experiment was
conducted to determine how time-dependent (i.e., day
versus night) lipopolysaccharide (LPS) challenge could
influence these skin mucosal defenses. The results
revealed that LD signal differentially modulated the
activities of mucosal immune molecules. Fish subjected
to LD regime showed significantly higher levels of skin
mucus lysozyme and protease at nighttime than at
daytime. This distinct feature was not observed in fish
under DD. There was no general mucosal response
patterns to time-dependent LPS challenge. Nonetheless,
protease and lysozyme, which were identified to be at
elevated levels at night, were significantly modulated
when the endotoxin was administered at nighttime.
Ceruloplasmin was the only molecule that responded to
LPS challenge at daytime, where its activity
significantly increased at 8 h post-stimulation.
Collectively, the results revealed that photoperiod
cues influenced the activities of mucosal defenses and
this may play, at least in part, in the temporal
sensitivity to bacterial endotoxin.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez:2019:EEA,
author = "Francisca P. Mart{\'\i}nez and Laura Berm{\'u}dez and
Mar{\'\i}a J. Aznar and Francisco J. Moyano",
title = "Evaluation of Enzyme Additives on the Nutritional Use
of Feeds with a High Content of Plant Ingredients for
\bioname{Mugil cephalus}",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "56",
day = "04",
month = dec,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040056",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/56",
abstract = "The Mugilidae are a group of fish with a great
interest for aquaculture due to their omnivorous
profile, rapid growth, and resistance to environmental
variations. The selection of feed ingredients for these
species is currently focused on an extensive use of
plant by-products, with this being limited by their
content in anti-nutritive factors (mainly phytate and
non-starch polysaccharides; NSPs). Nevertheless,
specific enzymes can be used to counteract some of
those negative effects. In the present study, the
effect of pretreating two high-plant feeds with a
mixture of enzymes (glucanases + phytase) on the
digestive use of protein and phosphorus by juvenile
mullets (\bioname{Mugil cephalus}) was assessed using
both in vitro and in vivo assays. The enzymatic
treatment significantly modified the potential
bioavailability of some nutrients, such as a reduction
of sugars, pentoses, and phytic phosphorus. Also, it
increased the digestibility of protein in one of the
feeds but reduced that of phosphorus in both of them.
The potential usefulness of enzyme treatment and the
information provided by the two types of assays are
discussed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sanchez-Nuno:2019:MPR,
author = "Sergio S{\'a}nchez-Nu{\~n}o and Sandra C. Silva and
Pedro M. Guerreiro and Borja Ord{\'o}{\~n}ez-Grande and
Ignasi Sanahuja and Laura Fern{\'a}ndez-Alacid and
Antoni Ibarz",
title = "Modulation of Pituitary Response by Dietary Lipids and
Throughout a Temperature Fluctuation Challenge in
Gilthead Sea Bream",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "55",
day = "22",
month = nov,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040055",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/55",
abstract = "Low temperatures provoke drastic reductions in
gilthead sea bream (\bioname{Sparus aurata}) activity
and nourishment, leading to growth arrest and a halt in
production. However, scarce data exist concerning the
implications of central core control during the cold
season. The aim of this work was to study the effects
of low temperature and recovery from such exposure on
the pituitary activity of sea bream juveniles fed 18\%
or 14\% dietary lipid. A controlled indoor trial was
performed to simulate natural temperature fluctuation
(22 {$^\circ $}C to 14 {$^\circ $}C to 22 {$^\circ
$}C). Meanwhile, we determined the regulatory role of
the pituitary by analyzing the gene expression of some
pituitary hormones and hormone receptors via qPCR, as
well as plasma levels of thyroidal hormones. In
response to higher dietary lipids, hormone pituitary
expressions were up-regulated. Induced low temperatures
and lower ingesta modulated pituitary function
up-regulating GH and TSH and thyroid and glucocorticoid
receptors. All these findings demonstrate the capacity
of the pituitary to recognize both external conditions
and to modulate its response accordingly. However,
growth, peripheral tissues and metabolism were not
linked or connected to pituitary function at low
temperatures, which opens an interesting field of study
to interpret the hypothalamus--pituitary--target axis
during temperature fluctuations in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Degani:2019:VBG,
author = "Gad Degani and Amir Alon and Akram Hajouj and Ari
Meerson",
title = "Vitellogenesis in Blue Gourami is Accompanied by Brain
Transcriptome Changes",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "54",
day = "29",
month = oct,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040054",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/54",
abstract = "The blue gourami (\bioname{Trichogaster trichopterus})
is a model for hormonal control of reproduction in
Anabantidae fish, but also relevant to other
vertebrates. We analyzed the female blue gourami brain
transcriptome in two developmental stages:
pre-vitellogenesis (PVTL) before yolk accumulation in
the oocytes, and high vitellogenesis (HVTL) at the end
of yolk accumulation in the oocytes. RNA sequencing of
whole-brain transcriptome identified 34,368 unique
transcripts, 23,710 of which could be annotated by
homology with other species. We focused on the
transcripts showing significant differences between the
stages. Seventeen and fourteen annotated genes were
found to be upregulated in PVTL and HVTL, respectively.
Five nuclear transcripts, three of which contain the
homeobox domain (ARX, DLX5, CERS6), were upregulated in
PVTL. Additionally, several receptors previously known
to be involved in reproduction were identified, and
three of these, G-protein coupled receptor 54, Membrane
progesterone receptor epsilon, and
Gonadotropin-releasing hormone II receptor (GPCR, mPR,
and GnRHR) were measured by quantitative RT-PCR in
brain, pituitary, and ovary samples from PVTL and HVTL
stage females. Of these, GPCR was highly expressed in
the brain and pituitary as compared to the ovary in
both PVTL and HVTL. GnRHR was highly expressed in the
ovary compared to the brain and pituitary, and its
levels in the brain were significantly higher in PVTL
than HVTL. Brain mPR mRNA levels were likewise higher
in PVTL than HVTL. In conclusion, this study details
changes in the female blue gourami brain transcriptome
through yolk accumulation in the oocytes and identifies
key genes that may mediate this process.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lobel:2019:GAP,
author = "Lisa Kerr Lobel and Devin M. Drown and Paul H. Barber
and Phillip S. Lobel",
title = "A Genetic Assessment of Parentage in the Blackspot
Sergeant Damselfish, \bioname{Abudefduf sordidus}
({Pisces: Pomacentridae})",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "53",
day = "24",
month = oct,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040053",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/53",
abstract = "Microsatellite markers were used to investigate the
reproductive behavior of the damselfish Abudefduf
sordidus at Johnston Atoll, Central Pacific Ocean.
Genetic results indicated that ten males maintained
guardianship over their nest territories for up to nine
nest cycles during a 3.5 month period. Genotypes of
1025 offspring sampled from 68 nests (composed of 129
clutches) were consistent with 95\% of the offspring
being sired by the guardian male. Offspring lacking
paternal alleles at two or more loci were found in 19
clutches, indicating that reproductive parasitism and
subsequent alloparental care occurred. Reconstructed
maternal genotypes allowed the identification of a
minimum of 74 different females that spawned with these
ten territorial males. Males were polygynous, mating
with multiple females within and between cycles.
Genetic data from nests, which consisted of up to four
clutches during a reproductive cycle, indicated that
each clutch usually had only one maternal contributor
and that different clutches each had different dams.
Females displayed sequential polyandry spawning with
one male within a cycle but switched males in
subsequent spawning cycles. These results highlight new
findings regarding male parasitic spawning, polygyny,
and sequential polyandry in a marine fish with
exclusive male paternal care.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Matsubara:2019:CSS,
author = "Hajime Matsubara and P. Mark Lokman and Yukinori
Kazeto and Hiromi Okumura and Shigeho Ijiri and
Toshiaki Hirai and Graham Young and Shinji Adachi and
Kohei Yamauchi",
title = "Changes in Sex Steroids and Ovarian Steroidogenic
Enzyme {mRNA} Levels in Artificially Maturing
\geoname{Japanese} Eel (\bioname{Anguilla japonica})
and Naturally Maturing New {Zealand} Longfin Eel
(\bioname{Anguilla dieffenbachii}) during
Vitellogenesis",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "52",
day = "17",
month = oct,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040052",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/52",
abstract = "Repeated hormone injections are routinely used to
induce advanced stages of oogenesis in freshwater eels,
but this approach may result in aberrant germ cell
development. To investigate the underlying causes,
levels of sex steroids (testosterone, T;
estradiol-17{\beta}, E2) and ovarian steroidogenic
enzyme mRNAs were compared between artificially
maturing Japanese eels and wild-caught, spontaneously
maturing New Zealand longfin eels. The latter were
employed as reference, as wild Japanese eels in
advanced stages of oogenesis are near-impossible to
catch. Serum T levels in artificially maturing Japanese
eel changed with stage in a pattern that was comparable
to that in longfin eels. Likewise, ovarian mRNA levels
of most steroidogenic enzyme genes were not
qualitatively dissimilar between both eel species when
taking developmental stage into account. However,
aromatase (cyp19a) mRNA levels, together with serum E2
levels, rapidly increased in artificially maturing
Japanese eels in mid-late stages of oogenesis
(gonadosomatic index, GSI = 13.8\%), whereas no such
increase was evident in longfin eels (GSI $ \approx $
6.9\%). In addition, sex steroid and target gene mRNA
levels fluctuated drastically with each hormone
injection. We contend that expression of most target
genes, possibly even that of cyp19a, during induced
oogenesis could be ``normal'', with the drastic
fluctuations due most likely to hormone delivery
through repeated injections. The effects of these
fluctuations on gamete quality remain unknown and
resolving this issue may prove fruitful in the future
to further artificial propagation of anguillid eels.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Papai:2019:NCB,
author = "N{\'o}ra P{\'a}pai and Ferenc Kagan and Gy{\"o}rgy
Csik{\'o}s and M{\'o}nika Kosztelnik and Tibor Vellai
and M{\'a}t{\'e} Varga",
title = "No Correlation between Endo- and Exoskeletal
Regenerative Capacities in Teleost Species",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "51",
day = "14",
month = oct,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040051",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/51",
abstract = "The regeneration of paired appendages in certain fish
and amphibian lineages is a well established and
extensively studied regenerative phenomenon. The
teleost fin is comprised of a proximal endoskeletal
part (considered homologous to the Tetrapod limb) and a
distal exoskeletal one, and these two parts form their
bony elements through different ossification processes.
In the past decade, a significant body of literature
has been generated about the biology of exoskeletal
regeneration in zebrafish. However, it is still not
clear if this knowledge can be applied to the
regeneration of endoskeletal parts. To address this
question, we decided to compare endo- and exoskeletal
regenerative capacity in zebrafish (\bioname{Danio
rerio}) and mudskippers (Periophthalmus barbarous). In
contrast to the reduced endoskeleton of zebrafish,
Periophthalmus has well developed pectoral fins with a
large and easily accessible endoskeleton. We performed
exo- and endoskeletal amputations in both species and
followed the regenerative processes. Unlike the almost
flawless exoskeletal regeneration observed in
zebrafish, regeneration following endoskeletal
amputation is often impaired in this species. This
difference is even more pronounced in Periophthalmus
where we could observe no regeneration in endoskeletal
structures. Therefore, regeneration is regulated
differentially in the exo- and endoskeleton of teleost
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Andrews:2019:CAS,
author = "Samuel N. Andrews and Sarah V. Hirtle and Tommi
Linnansaari and R. Allen Curry",
title = "Consumption of \geoname{Atlantic} Salmon Smolt by
Striped Bass: a Review of the Predator--Prey Encounter
Literature and Implications for the Design of Effective
Sampling Strategies",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "50",
day = "11",
month = oct,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040050",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/50",
abstract = "The native striped bass (\bioname{Morone saxatilis})
population of the Miramichi River, New Brunswick is
undergoing an unprecedented recovery while Atlantic
salmon (\bioname{Salmo salar}) numbers within that
system continue to decline. Atlantic salmon smolt
depart from the Miramichi system during the striped
bass spawning period and it is hypothesized that
elevated striped bass abundances will increase
encounter rates and predation on smolts. We summarize
all available striped bass diet studies occurring
within the native range of Atlantic salmon and present
a review of the feeding behavior and diet preferences
of striped bass before, during, and after their
spawning period. The key studies vary in methodologies
and interpretability. We present a standardized
approach for assessing striped bass predation threats
and smolt vulnerability and thus an improved
understanding of the species interactions to guide
future management in the Miramichi River.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Valdez:2019:ASC,
author = "Jose W. Valdez and Kapil Mandrekar",
title = "Assessing the Species in the {CARES Preservation
Program} and the Role of Aquarium Hobbyists in
Freshwater Fish Conservation",
journal = j-FISHES,
volume = "4",
number = "4",
pages = "49",
day = "29",
month = sep,
year = "2019",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes4040049",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/4/4/49",
abstract = "Freshwater fish represent half of all fish species and
are the most threatened vertebrate group. Given their
considerable passion and knowledge, aquarium hobbyists
can play a vital role in their conservation. CARES is
made up of many organizations, whose purpose is to
encourage aquarium hobbyists to devote tank space to
the most endangered and overlooked freshwater fish to
ensure their survival. We found the CARES priority list
contains nearly six hundred species from twenty
families and two dozen extinct-in-the-wild species. The
major families were typically those with the largest
hobbyist affiliations such as killifish, livebearers,
and cichlids, the latter containing half of CARES
species. CARES included every IUCN threatened species
of Pseudomugilidae and Valenciidae, but only one
percent of threatened Characidae, Cobitidae, and
Gobiidae species. No Loricariidae in CARES were in the
IUCN red list as they have not been scientifically
described. Tanzania and Mexico contained the largest
amount of species, with the latter containing the most
endemics. Many species were classified differently than
the IUCN, including a third of extinct-in-the-wild
species classified as least concern by the IUCN. This
vast disconnect exemplifies the importance of future
collaboration and information exchange required between
hobbyists, the scientific community, and conservation
organizations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ceballos-Francisco:2020:ELD,
author = "Diana Ceballos-Francisco and Alberto Cuesta and
Mar{\'\i}a {\'A}ngeles Esteban",
title = "Effect of Light--Dark Cycle on Skin Mucosal Immune
Activities of Gilthead Seabream (\bioname{Sparus
aurata}) and \geoname{European} Sea Bass
(\bioname{Dicentrarchus labrax})",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "10",
day = "24",
month = feb,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010010",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/10",
abstract = "Changes in different immune activities in the skin
mucus of gilthead seabream (\bioname{Sparus aurata}
{L.}) and European sea bass (\bioname{Dicentrarchus
labrax} {L.}) specimens exposed to a constant
light--dark photoperiod (12 h L:12 h D) were studied.
Samples were collected at 08:00 (light on), 14:00,
20:00 (light off), 02:00, and again at 08:00 to
determine immunoglobulin M (IgM) levels, several
enzymes related to the immune system, and bactericidal
activity. IgM levels were higher during the day in
seabream and reached a minimum value at 20:00, but it
was hardly affected in sea bass. No significant
variations were recorded in the levels of protease and
antiprotease. Peroxidase reached its maximum level in
seabream at 02:00, the same time that it reached its
minimum level in sea bass. Lysozyme showed little
variation in seabream, but it was significantly lower
at 14:00 than during the rest of the cycle in sea bass.
Finally, different interspecific variations on
bactericidal activity against \bioname{Vibrio harveyi}
were recorded. The findings demonstrate that the immune
parameters present in skin mucus of these important
fish species are affected by the light--dark cycle and
that there are substantial interspecies differences.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lopez:2020:SMC,
author = "Annalaura Lopez and Mauro Vasconi and Federica
Bellagamba and Tiziana Mentasti and Vittorio Maria
Moretti",
title = "Sturgeon Meat and Caviar Quality from Different
Cultured Species",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "9",
day = "07",
month = feb,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010009",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/9",
abstract = "Sturgeon raw eggs, caviar and meat obtained from
different species reared in an Italian production plant
were evaluated for their chemical composition, in order
to improve their appreciation on the market and to
detect any eventual distinctness related to the
species. Mainly, fatty acid (FA) profile of eggs and
caviar, determined by Gas-Chromatography coupled to
Flame Ionization Detection, showed variability in the
interspecific comparison, highlighted by chemometric
methods (Linear Discriminant Analysis). Generally, all
samples showed a prevalence of unsaturated fatty acids
with respect to saturated ones, reaching a content of
polyunsaturated fatty acids (PUFA) between the 40\% and
the 50\% of total FA. A remarkable presence of n3
series PUFA was detected in all samples and a selective
deposition of many FA into eggs' cellular membranes,
yolk lipid and body fat reserves, imputable to the
different biological role of single FA during sturgeon
reproduction, was evidenced. Chemical composition of
sturgeon flesh samples evidenced a high-protein and
medium-fat content, characterized by a FA profile of
high nutritional value. Moreover, color parameters
(redness, yellowness, brightness, Chroma) were measured
on sturgeon fillets, showing many species-specific
characteristics of sturgeon meat.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Office:2020:ARF,
author = "Fishes Editorial Office",
title = "Acknowledgement to Reviewers of Fishes in 2019",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "8",
day = "04",
month = feb,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010008",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/8",
abstract = "Sturgeon raw eggs, caviar and meat obtained from
different species reared in an Italian production plant
were evaluated for their chemical composition, in order
to improve their appreciation on the market and to
detect any eventual distinctness related to the
species. Mainly, fatty acid (FA) profile of eggs and
caviar, determined by Gas-Chromatography coupled to
Flame Ionization Detection, showed variability in the
interspecific comparison, highlighted by chemometric
methods (Linear Discriminant Analysis). Generally, all
samples showed a prevalence of unsaturated fatty acids
with respect to saturated ones, reaching a content of
polyunsaturated fatty acids (PUFA) between the 40\% and
the 50\% of total FA. A remarkable presence of n3
series PUFA was detected in all samples and a selective
deposition of many FA into eggs' cellular membranes,
yolk lipid and body fat reserves, imputable to the
different biological role of single FA during sturgeon
reproduction, was evidenced. Chemical composition of
sturgeon flesh samples evidenced a high-protein and
medium-fat content, characterized by a FA profile of
high nutritional value. Moreover, color parameters
(redness, yellowness, brightness, Chroma) were measured
on sturgeon fillets, showing many species-specific
characteristics of sturgeon meat.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sanchez-Gonzalez:2020:VTL,
author = "Jorge Rub{\'e}n S{\'a}nchez-Gonz{\'a}lez and Amadeo
Arbon{\'e}s and Frederic Casals",
title = "Variation over Time of Length--Weight Relationships
and Condition Factors for Four Exotic Fish Species from
a Restored Shallow Lake in {NE Iberian Peninsula}",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "7",
day = "04",
month = feb,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010007",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/7",
abstract = "Length--weight relationships (LWRs), condition
factors, and their variation over time were analyzed
for four exotic freshwater fish (bleak, common carp,
pikeperch, and roach) in the Estany d'Ivars i Vila-sana
shallow lake in Catalonia, Northern Spain. Fish samples
were collected twice a year (early summer and autumn),
between 2008 and 2016, by using between three and five
multi-mesh nylon gillnets. This study provides novel
information about four common exotic fishes outside of
their natural range and within the context of a
restored shallow lake, where the ichthyologic community
is evolving in concordance with the ecosystem
conditions and the fish community dynamics.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vijayakumar:2020:ICD,
author = "Parameswaran Vijayakumar and M. Leonor Cancela and
Vincent Laiz{\'e}",
title = "Isolation, Culture, and Differentiation of Blastema
Cells from the Regenerating Caudal Fin of Zebrafish",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "6",
day = "30",
month = jan,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010006",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/6",
abstract = "The caudal fin of teleost fish has become an excellent
system for investigating the mechanisms of epimorphic
regeneration. Upon amputation of the caudal fin, a mass
of undifferentiated cells, called blastema, proliferate
beneath the wound-epidermis and differentiate into
various cell types to faithfully restore the missing
fin structures. Here we describe a protocol that can be
used to isolate and culture blastema cells from
zebrafish. Primary cultures were initiated from 36 h
post-amputation (hpa) blastema and optimal cell growth
was achieved using L-15 medium supplemented with 5\%
fetal bovine serum in plates either coated with
fibronectin or uncoated. After seeding, zebrafish
blastema cells formed a uniform culture and exhibited
polygonal shapes with prominent nucleus, while various
cell types were also observed after few days in culture
indicating cell differentiation. Upon treatment with
all- trans retinoic acid, zebrafish blastema cells
differentiated into neuron-like and oligodendritic-like
cells. Immunocytochemistry data also revealed the
presence of mesenchymal and neuronal cells. The
availability of blastema cell cultures could contribute
to a better understanding of epimorphic regeneration by
providing a mean to investigate the mechanisms
underlying blastema cell differentiation. Furthermore,
this protocol is simple, rapid, and cost-efficient, and
can be virtually applied to the development of any fish
blastema cell culture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kampouris:2020:MRG,
author = "Thodoros E. Kampouris and Emmanouil Kouroupakis and
Ioannis E. Batjakas",
title = "Morphometric Relationships of the Global Invader
\bioname{Callinectes sapidus} {Rathbun}, 1896
({Decapoda, Brachyura, Portunidae}) from {Papapouli
Lagoon, NW Aegean Sea, Greece}. {With} Notes on Its
Ecological Preferences",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "5",
day = "14",
month = jan,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010005",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/5",
abstract = "\bioname{Callinectes sapidus} is native to the
Atlantic coasts of the Americas. In the Mediterranean,
it appeared around 1949 and though that it is
established in East Mediterranean waters, relevant
studies are limited. The aim of the present study is to
report quantitative and qualitative data on the blue
crab's biology and ecology in its non-native range,
that are indispensable for management purposes.
Papapouli Lagoon is in Thermaikos Gulf and is
ecologically impacted by the blue crabs. Fyke nets with
a 20 mm mesh opening were soaked for 12 to 14 h during
each survey. Abiotic environmental parameters were
obtained. The length--weight relationships were
expressed by the equation W = aL b. The species'
ecological preferences at Papapouli Lagoon were
assessed with PERMANOVA analysis, using the abiotic
parameters as factors. Student's t -tests were used to
assess the differences between sexes. The sex ratio of
the blue crab's population was assessed by a Chi-square
({\chi} 2) analysis. The sex ratio was found to be
1.28:1, in favour of males. The most dominant group
size of male blue crabs was the 61--70 mm of CL and
130--139 mm of CW. Also, the dominant size group of
female blue crabs was 60--69 mm of CL and 120--129 mm
CW. The maximum abundance of blue crabs was recorded at
a salinity range from 24{\SGMLpermil} to
25{\SGMLpermil} and the water temperature range was
from 26 to 28 {$^\circ $}C.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Young:2020:LHP,
author = "Alan M. Young and James A. Elliott",
title = "Life History and Population Dynamics of Green Crabs
(\bioname{Carcinus maenas})",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "4",
day = "31",
month = dec,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010004",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/4",
abstract = "Carcinus maenas (the ``shore crab'' or ``European
green crab'') is a very proficient invader (considered
to be one of the world's 100 worst invaders by the
IUCN) due to its phenotypic plasticity, wide
temperature and salinity tolerance, and an extensive
omnivorous diet. Native to Atlantic Europe, it has
established two well-studied nonindigenous populations
in the northwestern Atlantic and northeastern Pacific
and less-studied populations in Australia, Argentina
and South Africa. Green crabs are eurythermal and
euryhaline as adults, but they are limited to temperate
coastlines due to more restrictive temperature
requirements for breeding and larval development. They
cannot tolerate wave-swept open shores so are found in
wave-protected sheltered bays, estuaries and harbors.
\bioname{Carcinus maenas} has been the subject of
numerous papers, with over 1000 published in the past
decade. This review provides an up-to-date account of
the current published information on the life history
and population dynamics of this very important species,
including genetic differentiation, habitat preferences,
physical parameter tolerances, reproduction and larval
development, sizes of crabs, densities of populations,
sex ratios, ecosystem dynamics and ecological impacts
in the various established global populations of green
crabs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Baduy:2020:DRA,
author = "Flavia Baduy and Jo{\~a}o L. Saraiva and Filipe
Ribeiro and Adelino V. M. Canario and Pedro M.
Guerreiro",
title = "Distribution and Risk Assessment of Potential
Invasiveness of \bioname{Australoheros facetus}
({Jenyns}, 1842) in {Portugal}",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "3",
day = "27",
month = dec,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010003",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/3",
abstract = "Invasive species are recognized as a major cause of
biodiversity decline. Legal regulations relating to the
prevention, control, or eradication of invasive species
should always be up-to-date, as the failure to
recognize the problem, lack of adequate scientific
information, or long legal intervals required to
prepare the legislation may result in irreversible,
possibly catastrophic, outcomes. This implies constant
monitoring of the species distribution and levels of
establishment, as well as detailed knowledge about its
biology to predict dissemination and viability under
changing environmental conditions. Pre-screening kits
for potential invasive species are valuable tools for
policy makers, as they provide information about if and
how management measures should be taken. The Freshwater
Fish Invasiveness Scoring Kit (FISK) and the Aquatic
Species Invasiveness Screening Kit (AS-ISK) have been
suggested as reliable tools to assess the potential
risk of a species becoming invasive. The present study
highlights the spread of the non-native chameleon
cichlid \bioname{Australoheros facetus} in several
streams of the major river drainages in southern
Portugal and compares the fish assemblages and
ecological indices in two selected sites in the
Vasc{\~a}o and Odelouca rivers. We reviewed the current
knowledge on the distribution, physiology, and behavior
of A. facetus, and applied the toolkits FISK v2 and
AS-ISK to this species to evaluate whether the species
should be classified as invasive in Portugal. Field
data show high abundance of the species in most streams
and dominance in specific hotspots. The scores reached
by the kits (FISK v2: 23; AS-ISK: 37) places A. facetus
as a species with high potential of invasiveness and
support the recent inclusion of this species in the
invasive species list in Portugal (Decree-Law 92/2019),
but, most of all, highlights the importance of frequent
updates in both the field monitoring and the legal
regulation and watch lists of invasive organisms.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Carballo:2020:HEG,
author = "Carlos Carballo and Hyun Suk Shin and Concepci{\'o}n
Berbel and Maria Jes{\'u}s Zamorano and Juan Jose
Borrego and Eva Armero and Juan Manuel Afonso and
Manuel Manchado",
title = "Heritability Estimates and Genetic Correlation for
Growth Traits and {LCDV} Susceptibility in Gilthead Sea
Bream (\bioname{Sparus aurata})",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "2",
day = "25",
month = dec,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010002",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/2",
abstract = "The lymphocystis disease (LCD) is a viral infection
with a high economic impact in gilthead sea bream
aquaculture. In this study, genetic estimates
associated with lymphocystis disease virus (LCDV)
disease susceptibility and growth were determined in
sea bream juveniles. Two fish batches (named batch 1
and batch 2) were built from mass spawning and reared
under industrial conditions until disease outbreak. At
the moment of the sampling (n = 500 specimens for each
batch), all animals had the typical LCDV lesions in the
skin. For phenotyping, animals were weighted and
photographed for image analysis (surface covered and
lesion intensity). LCDV DNA copies were quantified in
the liver by qPCR. Batch 1 had a higher surface covered
and lesion intensity than batch 2, and the body caudal
region was the lowest affected region in both batches.
The average LCDV DNA copies in liver were higher in the
batch 1 than batch 2, and they were positively
correlated with severity index (SI) categories (r 2 =
0.90--0.94). The total number of families evaluated
were 150 and 128 for batch 1 and batch 2, respectively,
with a high bias in offspring contribution by family
and broodstock. Heritabilities for weight and length
were 0.18 and 0.14 in batch 1 and 0.06 and 0.05 in
batch 2, respectively. Heritability for the number of
viral DNA copies was low ($<$0.08) in both batches.
Heritabilities for SI in binary scale were 0.32/0.33
and 0.21/0.24 (underlying liability/Bayesian approach)
for batch 1 and batch 2, respectively. Genetic
correlations were very high and positive when growth
traits (weight and length) or disease traits (LCDV DNA
copies and SI) were compared. In contrast, the genetic
correlations between growth and disease traits were
moderate--high and positive in the batch 1 but negative
in batch 2. These results indicate the genetic
selection for LCDV susceptibility and growth is
feasible in sea bream juveniles, although estimates are
highly dependent on the age. The information provided
is relevant to designing selective breeding programs in
sea bream.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pimentel:2020:SLP,
author = "Marta S. Pimentel and Filipa Faleiro and Jorge Machado
and Pedro Pous{\~a}o-Ferreira and Rui Rosa",
title = "Seabream Larval Physiology under Ocean Warming and
Acidification",
journal = j-FISHES,
volume = "5",
number = "1",
pages = "1",
day = "20",
month = dec,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:24 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/1/1",
abstract = "The vulnerability of early fish stages represents a
critical bottleneck for fish recruitment; therefore, it
is essential to understand how climate change affects
their physiology for more sustainable management of
fisheries. Here, we investigated the effects of warming
(OW; +4 {$^\circ $}C) and acidification (OA; {\Delta}pH
= 0.5) on the heart and oxygen consumption rates,
metabolic enzymatic machinery---namely citrate synthase
(CS), lactate dehydrogenase (LDH), and
{\ss}-hydroxyacyl CoA dehydrogenase (HOAD), of seabream
(\bioname{Sparus aurata}) larvae (fifteen days after
hatch). Oxygen consumption and heart rates showed a
significant increase with rising temperature, but
decreased with p CO 2. Results revealed a significant
increase of LDH activity with OW and a significant
decrease of the aerobic potential (CS and HOAD
activity) of larvae with OA. In contrast, under OA, the
activity levels of the enzyme LDH and the LDH:CS ratio
indicated an enhancement of anaerobic pathways.
Although such a short-term metabolic strategy may
eventually sustain the basic costs of maintenance, it
might not be adequate under the future chronic ocean
conditions. Given that the potential for adaptation to
new forthcoming conditions is yet experimentally
unaccounted for this species, future research is
essential to accurately predict the physiological
performance of this commercially important species
under future ocean conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hsu:2020:PGA,
author = "Te-Hua Hsu and Chang-Wen Huang and Hung-Tai Lee and
Yi-Hsuan Kuo and Kwang-Ming Liu and Cheng-Hui Lin and
Hong-Yi Gong",
title = "Population Genetic Analysis for Stock Enhancement of
Silver Sea Bream (\bioname{Rhabdosargus sarba}) in
\geoname{Taiwan}",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "19",
day = "16",
month = jun,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020019",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/19",
abstract = "Stock enhancement is a method for replenishing
depleted wild finfish populations by supplementing them
with hatchery-raised fish. In Taiwan, silver sea bream
(\bioname{Rhabdosargus sarba}) is a predominant
commercial species involved in stock enhancement
projects. Although management agencies conduct stock
enhancement projects, there are a lot of private
releases without records. Stock enhancement is
performed by the private aquaculture sector without
accurate genetic records, potentially leading to
unintended consequences for wild populations. We
analyzed the genetics of 459 wild and 701
hatchery-reared specimens from nine batches produced by
various hatcheries. Wild and hatchery-reared samples
could be considered two separate clades by using a set
of stable and informative microsatellite markers
including type I (from gene introns and 3{\prime}UTR)
and type II markers (randomly picked up from genome).
Type I microsatellite markers could more sensitively
reflect the loss of genetic diversity more than type II
markers in the domestication process. All specimens
were considered native by using mtDNA COI and
microsatellites. The genetic composition of the wild
population is relatively simple, and the estimated low
contribution rate of the hatchery stock (1.3--10.9\%;
6--50/459) indicated a weak but significant genetic
effect of stock enhancement. Therefore, establishing
standards for the stock enhancement of silver sea bream
for more effective supplementation of wild populations
is imperative.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Koel:2020:YLE,
author = "Todd M. Koel and Jeffery L. Arnold and Patricia E.
Bigelow and Travis O. Brenden and Jeffery D. Davis and
Colleen R. Detjens and Philip D. Doepke and Brian D.
Ertel and Hayley C. Glassic and Robert E. Gresswell and
Christopher S. Guy and Drew J. MacDonald and Michael E.
Ruhl and Todd J. Stuth and David P. Sweet and John M.
Syslo and Nathan A. Thomas and Lusha M. Tronstad and
Patrick J. White and Alexander V. Zale",
title = "{Yellowstone Lake} Ecosystem Restoration: a Case Study
for Invasive Fish Management",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "18",
day = "12",
month = jun,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/18",
abstract = "Invasive predatory lake trout \bioname{Salvelinus
namaycush} were discovered in Yellowstone Lake in 1994
and caused a precipitous decrease in abundance of
native Yellowstone cutthroat trout
\bioname{Oncorhynchus clarkii bouvieri}. Suppression
efforts (primarily gillnetting) initiated in 1995 did
not curtail lake trout population growth or lakewide
expansion. An adaptive management strategy was
developed in 2010 that specified desired conditions
indicative of ecosystem recovery. Population modeling
was used to estimate effects of suppression efforts on
the lake trout and establish effort benchmarks to
achieve negative population growth ({\lambda} {$<$} 1).
Partnerships enhanced funding support, and a scientific
review panel provided guidance to increase suppression
gillnetting effort to $>$46,800 100-m net nights; this
effort level was achieved in 2012 and led to a
reduction in lake trout biomass. Total lake trout
biomass declined from 432,017 kg in 2012 to 196,675 kg
in 2019, primarily because of a 79\% reduction in
adults. Total abundance declined from 925,208 in 2012
to 673,983 in 2019 but was highly variable because of
recruitment of age-2 fish. Overall, 3.35 million lake
trout were killed by suppression efforts from 1995 to
2019. Cutthroat trout abundance remained below target
levels, but relative condition increased, large
individuals ($>$ 400 mm) became more abundant, and
individual weights doubled, probably because of reduced
density. Continued actions to suppress lake trout will
facilitate further recovery of the cutthroat trout
population and integrity of the Yellowstone Lake
ecosystem.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{McColl:2020:BCC,
author = "Kenneth A. McColl and Agus Sunarto",
title = "Biocontrol of the Common Carp (\bioname{Cyprinus
carpio}) in \geoname{Australia}: a Review and Future
Directions",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "17",
day = "02",
month = jun,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020017",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/17",
abstract = "Invasive pest species are recognized as one of the
important drivers of reduced global biodiversity. In
Australia, the 267 invasive plant, animal and microbial
species, established since European colonization in the
1770s, have been unequivocally declared the most
important threat to species diversity in this country.
One invasive pest, the common carp (\bioname{Cyprinus
carpio}), has been targeted in an integrated pest
management plan that might include cyprinid herpesvirus
3 (CyHV-3) as a potential biocontrol agent. The
species-specificity of the released virus (and of field
variants that will inevitably arise) has been assessed,
and the virus judged to be safe. It has also been
hypothesised that, because the virulence of the CyHV-3
will likely decline following release, the virus should
be used strategically: initially, the aim would be to
markedly reduce numbers of carp in naive populations,
and then some other, as yet uncertain, complementary
broad-scale control measure would knock-down carp
numbers even further. Brief results are included from
recent studies on the modelling of release and spread
of the virus, the ecological and social concerns
associated with virus release, and the restoration
benefits that might be expected following carp control.
We conclude that, while further work is required (on
the virus, the target species, environmental issues,
and especially the identification of a suitable
broad-scale complementary control measure), optimism
must prevail in order to ensure an eventual solution to
this important environmental problem.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Reyes:2020:NGE,
author = "Mar{\'\i}a Reyes and Mar{\'\i}a Rodr{\'\i}guez and
Juan Montes and Fernando G. Barroso and Dmitri Fabrikov
and Elvira Morote and Mar{\'\i}a Jos{\'e}
S{\'a}nchez-Muros",
title = "Nutritional and Growth Effect of Insect Meal Inclusion
on Seabass (\bioname{Dicentrarchuss labrax}) Feeds",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "16",
day = "01",
month = jun,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/16",
abstract = "Abajo: se repite los tres en el resumen. This work
studies the effect of high-level fish meal replacement
with insect meal: YW meal (obtained from Tenebrio
larvae fed a broiler diet), BSF meal (from hermetia
larvae fed broilers diet), BSFm meal (obtained from
hermetia larvae fed discard fish) on growth performance
nutritive indices and in vitro digestibility of
\bioname{Dicentrarchus labrax} juvenile. Three
different insect meals were used: BSF meal from
hermetia larvae fed broilers diet; BSF improve (BSFm)
obtained from hermetia larvae fed discarded fish; YW
meal obtained from the larvae of Tenebrio fed a broiler
diet. Five diets were used, a control (C) diet and four
experimental diets by replacing fishmeal with insect
meal from BSF at 30\% and 50\% (BSF30 and BSF50)
substitutions, BSFm at 50\% substitution (BSF50 m) and
YM at 50\% substitution (YW50). Nutritional and growth
indices worsened by including insect meal, especially
for hermetia meal at 50\% substitution, BSF50 and BSF50
m. The internal organs' weight reflected the growth of
the fish fed each experimental diet. No differences
were found in fillet composition. Nevertheless, under
our experimental condition, YW replacement obtained
better results than both BSF diets.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zaragoza:2020:SFS,
author = "Patricia Zaragoz{\'a} and Silvia Mart{\'\i}nez-Llorens
and Isabel Fern{\'a}ndez-Segovia and Jos{\'e}-Luis
Vivancos and Ana Tomas-Vidal and Ana Fuentes and
Jos{\'e} Vicente Ros-Lis and Ram{\'o}n
Mart{\'\i}nez-M{\'a}{\~n}ez and Jos{\'e} Manuel Barat",
title = "Study of Fishmeal Substitution on Growth Performance
and Shelf-Life of Giltheadsea Bream
(\bioname{Sparusaurata})",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "15",
day = "15",
month = may,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020015",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/15",
abstract = "In this work the effect of partial or total
replacement of fishmeal by plant protein sources and
krill and squid meal on growth performance and
shelf-life of gilthead sea bream was evaluated. Plant
protein dietswith 50 g kg {-1} of krill and 100 g kg
{-1} of squid were supplemented with synthetic amino
acids and at the end of the growing period weight
showed no significant differences. The spoilage process
of the fish was followed by physicochemical and
microbiological measurements together with a
colorimetric sensor array (CSA) specially designed for
that purpose. The changes in the physicochemical
parameters and microbial growth showed that shelf-life
of samples were in all cases lower than ninedays. The
CSA was not able to show significant differences
between both diets, confirming the physicochemical and
microbiological results. The fact that the type of feed
had no effect on the freshness parameters studied
demonstrates that total fishmeal replacement with plant
protein blends in the proportions used in this work
could be an excellent alternative for feed formulation
in aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dam:2020:AFR,
author = "Chinh Thi My Dam and Mark Booth and Igor Pirozzi and
Michael Salini and Richard Smullen and Tomer Ventura
and Abigail Elizur",
title = "Alternative Feed Raw Materials Modulate Intestinal
Microbiota and Its Relationship with Digestibility in
Yellowtail Kingfish \bioname{Seriola lalandi}",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "14",
day = "11",
month = may,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020014",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/14",
abstract = "Gut microbiota plays a crucial role in nutrient
digestibility and fish health. This study aimed to
investigate the effects of alternative feed raw
materials on the bacterial communities in the distal
intestine and its relationship with nutrient
digestibility in yellowtail kingfish (YTK),
\bioname{Seriola lalandi}. Two 4-week digestibility
trials were conducted to evaluate fish meal (FM), two
sources of poultry by-product meal (PBM-1 \& PBM-2),
blood meal (BLM), faba bean meal (FBM), corn gluten
meal (CGM), soy protein concentrate (SPC) and wheat
flour (WH). The nutrient digestibility value was
determined using the stripping fecal collection method.
Bacterial communities were characterized by
high-throughput sequencing based on V3-V4 region of the
16S rRNA gene. The most abundant phylum identified in
the present study was Proteobacteria. A significant
change in the distal intestine was observed in fish fed
diets containing CGM and BLM, characterized by a
reduction of species richness and diversity.
Additionally, significant correlation between nutrient
digestibility and intestinal microbiota was observed.
Allivibrio, Vibrio, Curvibacter, Ruminococcaceae, and
Clostridium were positively correlated, whereas
Ralstonia genus was negatively correlated with nutrient
digestibility. This study demonstrated that intestinal
microbiota could be a useful tool for evaluating the
digestibility of feed raw materials; however, further
culture-based study is needed to confirm this
observation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lindholm-Lehto:2020:AGM,
author = "Petra Lindholm-Lehto and Juha Koskela and Janne Kaseva
and Jouni Vielma",
title = "Accumulation of Geosmin and 2-methylisoborneol in
\geoname{European} Whitefish \bioname{Coregonus
lavaretus} and Rainbow Trout \bioname{Oncorhynchus
mykiss} in {RAS}",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "13",
day = "11",
month = may,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020013",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/13",
abstract = "Geosmin (GSM) and 2-methylisoborneol (MIB)-induced
off-flavors can cause serious problems in a
recirculating aquaculture system (RAS), such as delayed
harvest and increased production costs, but also damage
producers' reputation. Traditionally, off-flavors have
been removed by depuration before harvesting. Rainbow
trout (\bioname{Oncorhynchus mykiss}) and European
whitefish (\bioname{Coregonus lavaretus}) are
commercially valuable species produced for consumers,
both being suitable for rearing in RAS. In this study,
European whitefish and rainbow trout were raised from
juvenile up to 240 g (European whitefish) and 660 g
(rainbow trout) to monitor the long-term accumulation
of off-flavors. The concentrations in fillet of rainbow
trout reached 3.6 ng{\cdot}g {-1} (MIB) and 5.6 ng$
\bullet $ g {-11} (GSM) with lipid content of 22.5\%,
while for European whitefish up to 3.2 ng{\cdot}g {-1}
(MIB) and 3.9 ng{\cdot}g {-1} (GSM) were found with
14.8\% in lipid content. Concentrations up to 58
ng{\cdot}L {-1} (MIB) and 49 ng{\cdot}L {-1} (GSM) were
found in the circulating water. Based on the results,
the accumulation of MIB proceeds at similar pace for
both species. In the case of GSM, the accumulation
started similarly for both species but proceeded more
quickly for rainbow trout after 140 days of the
experiment, with a statistically significant difference
(p {$<$} 0.05).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dunker:2020:DRA,
author = "Kristine Dunker and Robert Massengill and Parker
Bradley and Cody Jacobson and Nicole Swenson and Andy
Wizik and Robert DeCino",
title = "A Decade in Review: \geoname{Alaska}'s Adaptive
Management of an Invasive Apex Predator",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "12",
day = "21",
month = apr,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020012",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/12",
abstract = "Northern pike are an invasive species in southcentral
Alaska and have caused the decline and extirpation of
salmonids and other native fish populations across the
region. Over the last decade, adaptive management of
invasive pike populations has included population
suppression, eradication, outreach, angler engagement,
and research to mitigate damages from pike where
feasible. Pike suppression efforts have been focused in
open drainages of the northern and western Cook Inlet
areas, and eradication efforts have been primarily
focused on the Kenai Peninsula and the municipality of
Anchorage. Between 2010 and 2020, almost 40,000 pike
were removed from southcentral Alaska waters as a
result of suppression programs, and pike have been
successfully eradicated from over 20 lakes and creeks
from the Kenai Peninsula and Anchorage, nearly
completing total eradication of pike from known
distributions in those areas. Northern pike control
actions are tailored to the unique conditions of waters
prioritized for their management, and all efforts
support the goal of preventing further spread of this
invasive aquatic apex predator to vulnerable waters.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cerbule:2020:SLB,
author = "Kristine Cerbule and Jacques Godfroid",
title = "Salmon Louse (\bioname{Lepeophtheirus salmonis}
({Kr{\o}yer})) Control Methods and Efficacy in
{Atlantic Salmon} (\bioname{Salmo salar} ({Linnaeus}))
Aquaculture: a Literature Review",
journal = j-FISHES,
volume = "5",
number = "2",
pages = "11",
day = "31",
month = mar,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5020011",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/2/11",
abstract = "The salmon louse (\bioname{Lepeophtheirus salmonis})
causes problems in Atlantic salmon (\bioname{Salmo
salar}) aquaculture in the Northern Hemisphere, because
infestations can result in both a loss of production
and in fish mortality. Several types of treatment have
been used to control louse infestations, but these have
seen varying success. The aim of this review is to
examine the efficacy and safety of commonly used
treatments (chemical, biological, mechanical, and
preventive measures) as documented in peer-reviewed
publications. Efficacy is assessed in relation to a
reduction in numbers of lice, and safety is assessed as
a lack of negative treatment-associated effects on fish
health and welfare (Atlantic salmon and/or cleaner
fish). Most chemical treatments showed decreasing
efficacy over time, together with the use of increasing
concentrations as a result of the development of
resistance to the treatments by lice. The need for a
restrictive use of pesticides to preserve treatment
efficacy has been emphasized. The use of cleaner fish
was suggested to be effective, with few or no negative
effects towards Atlantic salmon. The use of cleaner
fish would be preferable to chemical treatment if the
farmed fish health and welfare criteria are met. At
present, the number of peer-reviewed publications
relating to other forms of treatment and prevention are
sparse.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mulokozi:2020:EEA,
author = "Deogratias Pius Mulokozi and H{\aa}kan Berg and
Torbj{\"o}rn Lundh",
title = "An Ecological and Economical Assessment of Integrated
Amaranth (\bioname{Amaranthus hybridus}) and
\geoname{Nile} Tilapia (\bioname{Oreochromis
niloticus}) Farming in \geoname{Dar es Salaam,
Tanzania}",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "30",
day = "18",
month = sep,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030030",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/30",
abstract = "Organic wastes can be recycled in an ecologically
sound way in fishponds by applying integrated
agriculture and aquaculture systems (IAA). This kind of
waste recycling can help to protect the environment
from pollution and improve fishpond yields.
Additionally, IAA provides an opportunity for
diversification of the output from two or more existing
subsystems leading to higher overall farm economic
returns. This study explored the potential application
of amaranth wastes (AW) as a dietary ingredient for
tilapia in a tilapia-amaranths integrated system (ITA).
An experimental diet (AD) contained 10\% (based on the
control diet, CD) inclusion of AW collected from a
nearby vegetable market. The experiments included
triplicate treatments with; (i) fish fed on AD, where
the pond water was used for irrigating the amaranth
plants (IAA-fish), and (ii) fish fed on CD, where no
pond water was used for irrigating the amaranth plants
(non-IAA fish). 90 days after fish stocking, eighteen 4
m 2 amaranth plots were prepared and treated with (i)
tap water without fertilization (control amaranths),
(ii) water from IAA-fish pond and organically
fertilized (IAA amaranths), and (iii) tap water and
inorganically fertilized (non-IAA amaranths). The use
of AW improved the fish feed conversion ratio. The
overall net income from ITA was 3.2, 2.3, 2.6, and 1.8
higher than from non-IAA amaranths, IAA-amaranths,
non-IAA fish, and IAA fish sub-systems respectively.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bouska:2020:GSH,
author = "Wesley W. Bouska and David C. Glover and Jesse T.
Trushenski and Silvia Secchi and James E. Garvey and
Ruairi MacNamara and David P. Coulter and Alison A.
Coulter and Kevin Irons and Andrew Wieland",
title = "Geographic-Scale Harvest Program to Promote
Invasivorism of Bigheaded Carps",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "29",
day = "01",
month = sep,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030029",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/29",
abstract = "Invasive bigheaded carps, genus Hypophthalmichthys,
are spreading throughout the Mississippi River basin.
To explore the efficacy of a consumer-based market
(i.e., invasivorism) to manage them, we developed a
conceptual model and evaluated three harvest
approaches---direct contracted removal, volume-based
incentives (``fisher-side'' control), and set-quota
harvest (``market-side'' control). We quantified the
efficacy of these approaches and potential population
impact in the Illinois River. Contracted removal was
effective for suppressing small populations at the edge
of the range but cannot support a market.
``Fisher-side'' removals totaled 225,372 kg in one
year. However, participation was low, perhaps due to
reporting requirements for fishers. The
``market-side'', set-quota approach removed $>$1.3
million kg of bigheaded carp in less than 6 months.
Larger, older fish were disproportionately harvested,
which may hinder the ability to suppress population
growth. Total density declined in one river reach, and
harvest may reduce upstream movement toward the
invasion fronts. With sufficient market demand, harvest
may control bigheaded carp. However, lack of processing
infrastructure and supply chain bottlenecks could
constrain harvest, particularly at low commodity
prices. Given the geographical scale of this invasion
and complicated harvest logistics, concerns about
economic dependence on invasivorism that encourage
stock enhancement are likely unmerited.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wiryawan:2020:CPU,
author = "Budy Wiryawan and Neil Loneragan and Ulfah Mardhiah
and Sonja Kleinertz and Prihatin Ika Wahyuningrum and
Jessica Pingkan and Wildan and Putra Satria Timur and
Deirdre Duggan and Irfan Yulianto",
title = "Catch per Unit Effort Dynamic of Yellowfin Tuna
Related to Sea Surface Temperature and Chlorophyll in
{Southern Indonesia}",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "28",
day = "31",
month = aug,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030028",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/28",
abstract = "Tuna fisheries are the most valuable fisheries in the
world, with an estimated market value of at least
US\$42 billion in 2018. Indonesia plays an important
role in the global tuna fisheries and has committed to
improve its fisheries management; therefore, a pilot of
long-term spatial-temporal data bases was developed in
2012, however none have utilized data to have better
understanding for management improvement. In this
study, the annual and seasonal variation of large (\ge
10 kg) Yellowfin Tuna (YFT) catch per unit effort
(CPUE) have been investigated and the influence of sea
surface temperature (SST) and chlorophyll- a on these
variables examined. We used fish landing data from West
Nusa Tenggara recorded every month between 2012 and
2017 and analyzed using generalized linear models and
generalized additive models. We found a seasonal and
annual pattern of tuna abundance affected by SST and
chlorophyll- a (chl a) and related to upwelling and El
Nino event. These results also suggest that a two-month
closure to fishing in August and September in southern
Lombok is worth considering by the Government to
maximize conservation of stocks due to a high abundance
of juveniles emerging during the upwelling months from
June to August.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hayer:2020:UED,
author = "Cari-Ann Hayer and Michael F. Bayless and Amy George
and Nathan Thompson and Catherine A. Richter and Duane
C. Chapman",
title = "Use of Environmental {DNA} to Detect Grass Carp
Spawning Events",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "27",
day = "27",
month = aug,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030027",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/27",
abstract = "The timing and location of spawning events are
important data for managers seeking to control invasive
grass carp populations. Ichthyoplankton tows for grass
carp eggs and larvae can be used to detect spawning
events; however, these samples can be highly
debris-laden, and are expensive and laborious to
process. An alternative method, environmental DNA
(eDNA) technology, has proven effective in determining
the presence of aquatic species. The objectives of this
project were to assess the use of eDNA collections and
quantitative eDNA analysis to assess the potential
spawning of grass carp in five reservoir tributaries,
and to compare those results to the more traditional
method of ichthyoplankton tows. Grass carp eDNA was
detected in 56\% of sampling occasions and was detected
in all five rivers. Concentrations of grass carp eDNA
were orders of magnitude higher in June, corresponding
to elevated discharge and egg presence. Grass carp
environmental DNA flux (copies/h) was lower when no
eggs were present and was higher when velocities and
discharge increased and eggs were present. There was a
positive relationship between grass carp eDNA flux and
egg flux. Our results support the further development
of eDNA analysis as a method to detect the spawning
events of grass carp or other rheophilic spawners.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bernet:2020:ZFI,
author = "Daniel Bernet and Thomas Wahli and Christoph K{\"u}ng
and Helmut Segner",
title = "Zooplankton Feeding Induces Macroscopical Gonad
Malformations in Whitefish (\bioname{Coregonus} ssp.)
from {Lake Thun, Switzerland}",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "26",
day = "20",
month = aug,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030026",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/26",
abstract = "Alterations in gonad morphology are widespread in wild
fish populations. Whitefish (Coregonus spp.) from Lake
Thun, Switzerland, display a high prevalence of
macroscopical gonad malformations including fusions to
the musculature, segmented gonads and intersex
condition. The aim of the present study was to evaluate
whether the gonad morphological changes in Lake Thun
whitefish are caused by genetic factors, environmental
factors (water, diet) or ``gene $ \times $
environment'' interaction. We performed two independent
experiments of a three-year duration in which we reared
whitefish from fertilization until adulthood and tested
the possible causative factors using a matrix design:
(i) genetics---comparing the prevalence of gonad
malformations in whitefish of different genetic origin
reared under identical environmental conditions (same
diet, same water, same experimental facility); (ii)
environment---comparing the prevalence of gonad
malformations in whitefish of the same genetic origin
reared in different water sources and/or fed with
different diets; and (iii) gene-environment
interaction---comparing the prevalence of gonad
morphological alterations in relation to the
combinations of genetics and environmental factors. Two
diets were used for the rearing experiments: either
zooplankton collected in Lake Thun which represents the
natural diet of whitefish, or an artificial dry food
which was used as control. The key finding of this
study is that the inducing factor of the gonad
malformations is contained in the zooplankton of Lake
Thun. Fish fed with this diet developed a significantly
higher prevalence of malformations than fish from any
other treatment, irrespective of the genetic origin
and/or the water source. This result could be repeated
in the two independent experiments. Importantly, the
prevalence values observed in the experimental fish fed
with Lake Thun zooplankton were similar to the
prevalence values recorded in free-ranging whitefish in
the lake. The findings of this study advance the
understanding of the causes of gonad morphological
alterations in wild fish populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Suski:2020:DCD,
author = "Cory D. Suski",
title = "Development of Carbon Dioxide Barriers to Deter
Invasive Fishes: Insights and Lessons Learned from
Bigheaded Carp",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "25",
day = "13",
month = aug,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030025",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/25",
abstract = "Invasive species are a threat to biodiversity in
freshwater. Removing an aquatic invasive species
following arrival is almost impossible, and preventing
introduction is a more viable management option.
Bigheaded carp are an invasive fish spreading
throughout the Midwestern {United States} and are
threatening to enter the Great Lakes. This review
outlines the development of carbon dioxide gas (CO 2)
as a non-physical barrier that can be used to deter the
movement of fish and prevent further spread. Carbon
dioxide gas could be used as a deterrent either to
cause avoidance (i.e., fish swim away from zones of
high CO 2), or by inducing equilibrium loss due to the
anesthetic properties of CO 2 (i.e., tolerance). The
development of CO 2 as a fish deterrent started with
controlled laboratory experiments demonstrating stress
and avoidance, and then progressed to larger field
applications demonstrating avoidance at scales that
approach real-world scenarios. In addition, factors
that influence the effectiveness of CO 2 as a fish
barrier are discussed, outlining conditions that could
make CO 2 less effective in the field; these factors
that influence efficacy would be of interest to
managers using CO 2 to target other fish species, or
those using other non-physical barriers for fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Harris:2020:PGA,
author = "Sheila C. Harris and W. Robert Cope and Isaac Wirgin
and Eric M. Hallerman",
title = "Population Genetic Assessment of Anadromous and
Resident Striped Bass (\bioname{Morone saxatilis}) in
the {Roanoke River System, Eastern United States}",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "24",
day = "07",
month = aug,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030024",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/24",
abstract = "Striped bass is the subject of important commercial
and sport fisheries in North America. The Roanoke River
drainage---especially Smith Mountain Lake, Leesville
Lake, and Kerr Reservoir---has popular recreational
striped bass fisheries. After construction of five
hydroelectric dams, populations became landlocked,
declined, and have been supplemented by stocking. A key
basis for responsibly augmenting populations is to
characterize genetic variation and incorporate the
findings into responsible hatchery and stocking
practices. Genetic variation at 12 microsatellite DNA
loci was evaluated among 837 striped bass representing
16 collections across the native range; populations
from rivers in South Carolina, North Carolina,
Chesapeake Bay, and Hudson River were screened to
provide context for assessing genetic structure within
the Roanoke system. Analysis of population genetic
differentiation showed landlocked Roanoke River striped
bass to be distinctive. Subject to genetic isolation,
high M ratios, and relatively low N e estimates suggest
loss of genetic variation, and relatedness analysis
showed heightened frequencies of related individuals.
These insights into population genetics, demographics,
and existing guidelines for broodstock acquisition and
mating designs can inform genetically cognizant
hatchery management and stocking for striped bass in
the Roanoke River drainage. In particular, we recommend
the use of larger numbers of breeders and factorial
mating designs to increase the genetic diversity of
propagated striped bass stocked within the Roanoke
River drainage.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Costa:2020:ZMN,
author = "Bruna Patricia Dutra Costa and Layana Aquino Moura and
Sabrina Alana Gomes Pinto and Monica Lima-Maximino and
Caio Maximino",
title = "Zebrafish Models in Neural and Behavioral Toxicology
across the Life Stages",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "23",
day = "31",
month = jul,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030023",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/23",
abstract = "The industry is increasingly relying on fish for
toxicity assessment. However, current guidelines for
toxicity assessment focus on teratogenicity and
mortality. From an ecotoxicological point of view,
however, these endpoints may not reflect the ``full
picture'' of possible deleterious effects that can
nonetheless result in decreased fitness and/or
inability to adapt to a changing environment, affecting
whole populations. Therefore, assessing sublethal
effects add relevant data covering different aspects of
toxicity at different levels of analysis. The impacts
of toxicants on neurobehavioral function have the
potential to affect many different life-history traits,
and are easier to assess in the laboratory than in the
wild. We propose that carefully-controlled laboratory
experiments on different behavioral domains---including
anxiety, aggression, and exploration---can increase our
understanding of the ecotoxicological impacts of
contaminants, since these domains are related to traits
such as defense, sociality, and reproduction, directly
impacting life-history traits. The effects of selected
contaminants on these tests are reviewed, focusing on
larval and adult zebrafish, showing that these
behavioral domains are highly sensitive to small
concentrations of these substances. These strategies
suggest a way forward on ecotoxicological research
using fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Glencross:2020:ASC,
author = "Brett D. Glencross and David Huyben and Johan W.
Schrama",
title = "The Application of Single-Cell Ingredients in
Aquaculture Feeds --- a Review",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "22",
day = "16",
month = jul,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030022",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/22",
abstract = "Single-cell ingredients (SCI) are a relatively broad
class of materials that encompasses bacterial, fungal
(yeast), microalgal-derived products or the combination
of all three microbial groups into microbial bioflocs
and aggregates. In this review we focus on those dried
and processed single-cell organisms used as potential
ingredients for aqua-feeds where the microorganisms are
considered non-viable and are used primarily to provide
protein, lipids or specific nutritional components.
Among the SCI, there is a generalised dichotomy in
terms of their use as either single-cell protein (SCP)
resources or single-cell oil (SCO) resources, with SCO
products being those oleaginous products containing 200
g/kg or more of lipids, whereas those products
considered as SCP resources tend to contain more than
300 g/kg of protein (on a dry basis). Both SCP and SCO
are now widely being used as protein/amino acid
sources, omega-3 sources and sources of bioactive
molecules in the diets of several species, with the
current range of both these ingredient groups being
considerable and growing. However, the different array
of products becoming available in the market, how they
are produced and processed has also resulted in
different nutritional qualities in those products. In
assessing this variation among the products and the
application of the various types of SCI, we have taken
the approach of evaluating their use against a set of
standardised evaluation criteria based around key
nutritional response parameters and how these criteria
have been applied against salmonids, shrimp, tilapia
and marine fish species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jung:2020:FDO,
author = "Joo-Young Jung and Soohwan Kim and Kyochan Kim and
Bong-Joo Lee and Kang-Woong Kim and Hyon-Sob Han",
title = "Feed and Disease at Olive Flounder
(\bioname{Paralichthys olivaceus}) Farms in
\geoname{Korea}",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "21",
day = "14",
month = jul,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030021",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/21",
abstract = "The objective of this study was to conduct field
experiments comparing formulated (extruded pellets
(E.P)) and raw-fish (moist pellets (M.P)) feeds at two
flounder aquaculture farms for six months to obtain the
basic data necessary for improving aquafarmers'
awareness of feed-quality issues and firmly establish
and expand the use of formulated feed. According to the
results, the M.P group was higher in weight gain, feed
efficiency ratio, protein efficiency ratio, and
specific growth rate and lower in mortality rate than
the E.P group. All cases of mortality were caused by
six kinds of disease, four of which (Vibrio sp.,
Edwardsiella sp., Streptococcus sp., and
Scuticociliates) were common to the two groups, whereas
Lymphocystis arose only in the M.P group and abdominal
inflation only in the E.P group at both farms. As for
mortality in the present experiment, the numbers were
1047 at Da-Hae farm and 1167 at Global farm, with more
fish dying in the E.P tanks than in the M.P tanks. By
multiplying the number of deaths by selling price, the
economic losses were \$9650 and \$10,756, respectively.
Therefore, it should be considered an urgent priority
to develop flounder-exclusive formulated feed for
improved digestion and absorption rate and also to
establish a water-quality-improvement management
plan.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vu:2020:OLF,
author = "Ngoc-Ut Vu and Truong-Giang Huynh",
title = "Optimized Live Feed Regime Significantly Improves
Growth Performance and Survival Rate for Early Life
History Stages of Pangasius Catfish
(\bioname{Pangasianodon hypophthalmus})",
journal = j-FISHES,
volume = "5",
number = "3",
pages = "20",
day = "28",
month = jun,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5030020",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/3/20",
abstract = "This study aimed to determine the optimal live feed
regime (i.e., initial feeding moment, density, and
frequency) for maximum growth and survival of pangasius
catfish (\bioname{Pangasianodon hypophthalmus}) early
life history stages. The first experiment assessed the
optimal initial feeding moment (30, 36, 42, and 48 h
post hatching, hph). The second experiment assessed
feeding density (3, 5, 8 and 11 individuals per mL,
ind/mL) at the optimal initial feeding moment (30 hph)
which was the best result from the first experiment.
The third experiment assessed optimal feeding frequency
(1, 2, 4, and 6 times per day) at the optimal initial
feeding moment (30 hph) and density (8 ind/mL) which
was drawn upon from the second experiment. All
experiments were conducted in 20 L containers
containing 20 hph P. hypophthalmus larvae at a density
of 10 ind/L and fed rotifers (Brachionus angularis) for
3 days and then water fleas (\bioname{Moina macrocopa})
for 7 days. The first experiment demonstrated that
larvae initially fed at 30 hph exhibited a
significantly higher survival rate (24\%) than larvae
initially fed at 36, 42, and 48 hph (19\%, 16\%, and
16\%), respectively. The second experiment demonstrated
that larvae fed at 8 and 11 ind/mL densities exhibited
significantly higher survival rates (32\% and 32\%)
than larvae fed at 3 and 5 ind/mL densities (13\% and
23\%), respectively. The third experiment demonstrated
that the highest survival rate (66\%) was obtained when
larvae were fed 6 times per day. These results provide
valuable insights regarding the optimal live feed
regime for better growth and survival of P.
hypophthalmus larvae, which are commercially important
and numerously cultured throughout the Mekong Delta
region.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nikiforidou:2020:VMM,
author = "Vasiliki Nikiforidou and Stefanos Zaoutsos and
Nikolaos Vlahos and Panagiotis Berillis",
title = "Vertebrae Morphometric Measurement and {Ca/P} Levels
of Different Age \geoname{European} Seabass
(\bioname{Dicentrarchus labrax})",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "37",
day = "08",
month = dec,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040037",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/37",
abstract = "The European seabass is one of the most important
species of the Mediterranean, specifically Greece.
Individuals with different numbers of vertebrae have
been reported. This number ranges from 24 to 26
vertebrae. In this study a sample of 73 individual
seabass were collected from fish farms and divided into
three age groups. The first group included fingerling
individuals, the second group, juvenile individuals and
the third group, adult individuals. The number and the
length of their vertebrae were measured by radiographs.
The individuals were divided into subgroups according
to their vertebrae number, and from each one the tenth
vertebra was taken. Ca and P levels (\%) of each tenth
vertebra were measured by X-ray spectroscopy (EDS), and
the Ca/P ratio was determined. Vertebrae length, Ca and
P levels and Ca/P ratio were compared among age groups
and among individuals with different numbers of
vertebrae. It was shown that the European seabass's
vertebral column can be divided to three
sections---cervical, abdominal and caudal---following
the striped bass (\bioname{Morone saxatilis}) model.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sorensen:2020:CSD,
author = "Peter W. Sorensen and Przemyslaw G. Bajer",
title = "Case Studies Demonstrate That Common Carp Can Be
Sustainably Reduced by Exploiting Source-Sink Dynamics
in {Midwestern} Lakes",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "36",
day = "04",
month = dec,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040036",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/36",
abstract = "The common carp has been highly problematic in North
American ecosystems since its introduction over a
century ago. In many watersheds, its abundance appears
to be driven by source-sink dynamics in which carp
reproduce successfully in peripheral ponds that lack
egg/larva micro-predators which then serve as sources
of recruits for deeper lakes. This manuscript describes
how carp were sustainably reduced in two chains of
lakes by disrupting source-sink dynamics in three
steps. First, we ascertained whether lakes had
problematic densities of carp that could be explained
by source-sink dynamics. Second, ways to control
recruitment were developed and implemented including:
(i) aerating source ponds to reduce hypoxia and
increase micro-predator abundance, (ii) blocking carp
migration, and (iii) locating and removing adults from
sinks using targeted netting guided by Judas fish.
Third, we monitored and adapted. Using this strategy,
the density of carp in 3 lakes in one chain was reduced
from 177 kg/ha to $ \approx $100 kg/ha in 3 years and
held constant for a decade. Similarly, adult density
was reduced from 300 kg carp/ha in 2 lakes in the other
chain to 25 kg/ha. Once carp densities were low,
aluminum sulfate treatments became reasonable and once
conducted, water quality improved.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Richard:2020:MFW,
author = "Adand{\'e} Richard and Liady Mouhamadou Nourou Dine
and Djidohokpin Gildas and Adjahouinou Dogb{\`e}
Cl{\'e}ment and Azon Mahuan Tobias C{\'e}saire and
Micha Jean-Claude and Fiogbe Didier Emile",
title = "Multispecies Fresh Water Algae Production for Fish
Farming Using Rabbit Manure",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "35",
day = "30",
month = nov,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040035",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/35",
abstract = "The current study aims at determining the optimal
usage conditions of rabbit manure in a multispecies
fresh water algae production for fish farming. This
purpose, the experimental design is made of six
treatments in triplicate including one control T 0, T
1, T 2, T 3, T 4, T 5 corresponding respectively to 0,
300, 600, 900, 1200, 1500 g/m 3 of dry rabbit manure
put into buckets containing 40 L of demineralized water
and then fertilized. The initial average seeding
density is made of 4 $ \times $ 10 3 {\pm} 2.5 $ \times
$ 10 2 cells/L of Chlorophyceae, 1.5 $ \times $ 10 3
{\pm} 1 $ \times $ 10 2 cells/L of Coscinodiscophyceae,
3 $ \times $ 10 3 {\pm} 1.2 $ \times $ 10 2 cells/L of
Conjugatophyceae, 2.8 $ \times $ 10 3 {\pm} 1.5 $
\times $ 10 2 cells/L of Bascillariophyceae, and 2.5 $
\times $ 10 3 {\pm} 1.4 $ \times $ 10 2 cells/L of
Euglenophyceae. During the experiments, the effects of
these treatments on abiotic and biotic parameters
(chlorophyll-a concentration, phytoplankton density and
algal density) of different production media were
monitored. Results show that average density of
different phytoplankton classes is higher in treatment
T 5 (7.91 $ \times $ 10 8 {\pm} 6.78 $ \times $ 10 7
cells/L) followed by T 4 (5.56 $ \times $ 10 8 {\pm}
4.27 $ \times $ 10 7 cells/L), T 2 (3.87 $ \times $ 10
8 {\pm} 3.10 $ \times $ 10 8 cells/L), T 3 (3.79 $
\times $ 10 8 {\pm} 3.18 $ \times $ 10 8 cells/L), with
high significant difference (F (4,84) = 5, 35, p {$<$}
0.00). Chl-a concentration varied from 0.07 {\pm} 0.05
mg/L (T 0) to 14.47 {\pm} 12.50 mg/L (T 5) with high
significant differences observed among treatments (F
(5,83) = 3,09, p = 0,01). In addition, fourteen (14)
species belonging to eight (8) families, five (5)
classes and three (3) phyla were identified in our
different production media. During the culture,
Chlorophyceae class was the most represented in all
treatments with 5 species (36\% of the specific
diversity) while Euglenophyceae class (7\%) was the
least represented with only one (01) species. According
to these results, treatments T 2 (600 g/m 3), T 3 (900
g/m 3) and T 4 (1200 g/m 3) of dry rabbit manure are
those worthy to be recommended as an alternative for a
low cost massive production of multispecies freshwater
algae that can be easily used by freshwater zooplankton
and macroinvertebrates. Indeed, despite the best
performances that it shows, treatment T 5, presents
important eutrophication's risks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ytteborg:2020:MTV,
author = "Elisabeth Ytteborg and {\O}yvind Johannes Hansen and
Vibeke H{\o}st and Sergey Afanasyev and Ireen Vieweg
and Jasmine Nahrgang and Aleksei Krasnov",
title = "Morphology, Transcriptomics and In Vitro Model of Skin
from Polar Cod (\bioname{Boreogadus saida}) and
\geoname{Atlantic} Cod (\bioname{Gadus morhua})",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "34",
day = "04",
month = nov,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040034",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/34",
abstract = "Fish skin is a multifunctional barrier tissue with
high regeneration capacity that interacts with the
surrounding environment and provides protection.
Functional importance, high complexity and activity
make skin an attractive tissue for studying the effects
of environmental challenges and chemical stressors in
fish. The aim of this work was to characterize skin
from polar cod (\bioname{Boreogadus saida}) and
Atlantic cod (\bioname{Gadus morhua}), and to test cod
skin as an in vitro model in exposure studies. Both
species have similar skin structures including
epidermis, mucous cells, club cells and scales.
However, microarchitectural differences were detected;
Atlantic cod has a smooth epidermal surface and
overlapping scales, whereas polar cod has a folded
outer surface and discontinuous scales. Genome-wide
microarray found 6.5k genes with expression
differences, which suggested more active turnover of
proteins, proliferation and motility of cells in skin
of polar cod. Hydrogen peroxide (H 2 O 2) was used to
examine skin responses. Transcriptome response was
stronger in the skin of polar cod, with 155
differentially expressed genes. The skin from Atlantic
cod was further used to develop a cell culture. H 2 O 2
decreased the cell migration rate in a dose-dependent
manner, which could indicate reduced skin healing
capacity. The results revealed novel skin structures
and confirmed that the skin from cod is a promising
tissue for evaluation of stressors.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Newman:2020:CBI,
author = "Raymond M. Newman and Fred G. Henson and Carl
Richards",
title = "Competition between Invasive Ruffe
(\bioname{Gymnocephalus cernua}) and Native Yellow
Perch (\bioname{Perca flavescens}) in Experimental
Mesocosms",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "33",
day = "17",
month = oct,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040033",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/33",
abstract = "Ruffe (\bioname{Gymnocephalus cernua}) were introduced
to North America from Europe in the mid-1980s and based
on similar diets and habit use may compete with yellow
perch (\bioname{Perca flavescens}). To examine
competitive interactions between invasive ruffe and
native yellow perch, individually marked perch and
ruffe were placed in mesocosms in a small lake.
Mesocosms allowed fish to interact and feed on the
natural prey populations enclosed. In the first
experiment, four treatments were assessed: 28 perch, 14
perch + 14 ruffe, 14 perch, and 7 perch + 7 ruffe.
Yellow perch growth was significantly lower in the
presence of ruffe (ANOVA, p = 0.005) than in treatments
containing only perch. In a second experiment, an
increasing density of one species was superimposed upon
a constant density of the other in parallel treatment
series. Growth rates of both ruffe and perch declined
when ruffe density was increased (t test, p = 0.006).
However, neither ruffe nor perch growth was affected by
increasing perch density. Total stomach content mass of
perch was significantly decreased by ruffe in both
years (p {$<$} 0.02), but no effects of ruffe on the
composition of perch diets were observed. Ruffe growth
and food consumption was greater than that of perch for
both experiments. Ruffe can outcompete yellow perch
when both species depend on a limited benthic food
resource. Thus there is reason for concern for the
ecological effects of ruffe if they expand their range
into {Lake Erie} or North American inland lakes that
contain yellow perch.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mmanda:2020:DLF,
author = "Francis Pius Mmanda and Jan Erik Lindberg and Anna
Norman Hald{\'e}n and Matern S. P. Mtolera and Rukia
Kitula and Torbj{\"o}rn Lundh",
title = "Digestibility of Local Feed Ingredients in Tilapia
\bioname{Oreochromis niloticus} Juveniles, Determined
on Faeces Collected by Siphoning or Stripping",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "32",
day = "15",
month = oct,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040032",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/32",
abstract = "Eight locally available protein source ingredients in
Tanzania were selected for assessment of apparent
digestibility (AD) in tilapia \bioname{Oreochromis
niloticus}, using faeces samples collected by siphoning
or stripping. The selected protein source ingredients
were Lake Victoria sardines (FM), brewers spent yeast
(BSY), moringa leaves (ML), freshwater shrimp (FSH),
marine shrimp (MSH), cattle blood (CB), duckweed (DW)
and fish frames (FF). The AD (\%) of dry matter (DM),
organic matter (OM) and crude protein (CP) was
unaffected (p $>$ 0.782--0.901) by the faeces
collection method (i.e., siphoning or stripping), with
correlation coefficient (r) of 0.98, 0.99 and 0.93
between AD values for DM, OM and CP, respectively,
following siphoning and stripping. The AD (\%) of DM,
OM, CP and gross energy (GE) in the test ingredients
differed (p {$<$} 0.0001). The AD (\%) of DM and OM was
lowest in BSY and DW, followed in increasing order by
ML, MSH, FF, FSH and CB. In general, the AD (\%) of CP
was high ($>$76\%), but with a low value (46\%) for DW.
The AD (\%) of GE was closely correlated (r = 0.96)
with the AD of OM. In conclusion, FSH, MSH, CB, FF, BSY
and ML have acceptable protein digestibility to be used
in tilapia diet formulation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ruiz-Garcia:2020:DSM,
author = "David Ruiz-Garc{\'\i}a and Kye Adams and Heidi Brown
and Andrew R. Davis",
title = "Determining Stingray Movement Patterns in a Wave-Swept
Coastal Zone Using a Blimp for Continuous Aerial Video
Surveillance",
journal = j-FISHES,
volume = "5",
number = "4",
pages = "31",
day = "30",
month = sep,
year = "2020",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes5040031",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:25 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/5/4/31",
abstract = "Stingrays play a key role in the regulation of
nearshore ecosystems. However, their movement ecology
in high-energy surf areas remains largely unknown due
to the notorious difficulties in conducting research in
these environments. Using a blimp as an aerial platform
for video surveillance, we overcame some of the
limitations of other tracking methods, such as the use
of tags and drones. This novel technology offered
near-continuous coverage to characterise the fine-scale
movements of stingrays in a surf area in Kiama,
Australia, without any invasive procedures. A total of
98 stingray tracks were recorded, providing 6 h 27 min
of movement paths. The tracking data suggest that
stingrays may use a depth gradient located in the
sandflat area of the bay for orientating their
movements and transiting between locations within their
home range. Our research also indicates that stingray
behaviour was influenced by diel periods and tidal
states. We observed a higher stingray occurrence during
the afternoon, potentially related to foraging and
anti-predatory strategies. We also saw a reduced route
fidelity during low tide, when the bathymetric
reference was less accessible due to stranding risk.
Considering the increasing threat of anthropogenic
development to nearshore coastal environments, the
identification of these patterns can better inform the
management and mitigation of threats.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Setyohadi:2021:UIS,
author = "Daduk Setyohadi and Umi Zakiyah and Abu Bakar Sambah
and Adi Wijaya",
title = "Upwelling Impact on \bioname{Sardinella lemuru} during
the \geoname{Indian Ocean} Dipole in the {Bali Strait,
Indonesia}",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "8",
day = "16",
month = mar,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010008",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/8",
abstract = "Understanding the impact of Indian Ocean Dipole (IOD)
on fishery around Indonesia is important as the fishery
resources are small compared to the demand. In this
study, we analyzed the effect of positive and negative
phases of IOD on chlorophyll-a concentration and the
catch of Sardinella lemuru in the Bali Strait. Data are
based on field surveys in the Bali Strait during the
positive and negative phase of IOD and Sea Surface
Temperature (SST) and Sea Surface Chlorophyll (SSC)
obtained from the analysis of satellite images. The
results suggest that SSC concentration in the strait
significantly correlates with the positive and negative
phase of IOD, possibly through a change of upwelling
and downwelling there. It is suggested that the change
of phytoplankton biomass due to positive IOD would
result in an increase of \bioname{Sardinella lemuru} in
the Bali Strait. This research has direct implications
as important information for the government in planning
lemuru fisheries management in the Bali Strait based on
oceanographic studies and climate phenomena.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2021:SIS,
author = "Xi Zhang and Zhiyuan Shen and Tianpeng Qi and Rujuan
Xi and Xiao Liang and Li Li and Rong Tang and Dapeng
Li",
title = "Slight Increases in Salinity Improve Muscle Quality of
Grass Carp (\bioname{Ctenopharyngodon idellus})",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "7",
day = "24",
month = feb,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010007",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/7",
abstract = "Fish muscle quality is an important parameter in the
aquaculture industry. In this study, we analyzed and
compared the muscle quality of grass carp
(\bioname{Ctenopharyngodon idellus}) cultured at
salinities of 0\permille, 3\permille, and 6\permille
(GC0, GC3, GC6). There was no significant difference in
crude protein and crude fat content of muscle between
GC0 and GC3. Crude fat was significantly lower in GC6
compared to the other groups. GC3 and GC6 had higher
hydroxyproline content, which suggested that these
groups had higher collagen content. GC3 and GC6 had
higher contents of free amino acids and umami amino
acids than GC0, but there was no significant difference
in sweet or sour amino-acid content among groups. GC3
and GC6 had better texture properties, including
hardness, gumminess, chewiness, resilience, and
springiness, than GC0. GC3 had the highest
water-holding capacity among the groups. As the
salinity increased, the diameter of muscle fibers
decreased and the sarcolemma showed a thickening trend.
These results suggest that a slight increase in
salinity (i.e., 3\permille) can effectively improve the
muscle quality of grass carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yick:2021:EIC,
author = "Jonah L. Yick and Chris Wisniewski and John Diggle and
Jawahar G. Patil",
title = "Eradication of the Invasive Common Carp,
\bioname{Cyprinus carpio} from a Large Lake: Lessons
and Insights from the \geoname{Tasmanian} Experience",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "6",
day = "23",
month = feb,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010006",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/6",
abstract = "Common carp (\bioname{Cyprinus carpio}, L. 1758) are
the most abundant pest fish species in Australia,
detrimental to ecosystem integrity and values, and in
need of suitable management solutions. In January 1995,
this destructive pest was discovered in two large,
connected Tasmanian lakes --- Lakes Crescent (23 km 2)
and Sorell (54 km 2). After an initial assessment, carp
were immediately contained to these waters using
screens to prevent their escape down-stream, followed
by swift legislation to enforce closure of the lakes to
the public. Assessment and evaluation of carp numbers
occurred throughout the eradication program, with
effort focused on Lake Crescent. Beginning with
undirected removal, techniques progressively evolved to
more sophisticated targeted removal with assistance
from biotelemetry, in conjunction with gill netting and
electro-fishing. Real-time population estimates and in
situ observations resulted in a detailed cumulative
understanding of carp population dynamics, behaviour
and seasonal habitat choice. This allowed strategic
deployment of fences to block access to marshes, and
the installation of steel traps within the fences.
These gears specifically prevented spawning
opportunities, while concurrently capturing mature
fish. Following 12 years of adaptive and integrated
effort, 7797 carp (fry, juvenile and adult) were
captured from Lake Crescent, with the last carp being
caught in December 2007. The subsequent 14 years of
monitoring has not resulted in the capture of any carp,
confirming the successful eradication of carp from Lake
Crescent. These management practices have been
successfully replicated in the larger Lake Sorell,
where 41,499 carp (fry, juvenile and adult) have been
removed. It is now estimated that there are few, if any
carp remaining. Collectively, the techniques and
strategies described here were reliable, and can be
applied as a model to control or eradicate pest
populations of carp in freshwater lakes elsewhere.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez:2021:SGD,
author = "Gil Mart{\'\i}nez and Emyr Pe{\~n}a and Rafael
Mart{\'\i}nez and Susana Camarillo and Warren Burggren
and Alfonso {\'A}lvarez",
title = "Survival, Growth, and Development in the Early Stages
of the Tropical Gar \bioname{Atractosteus tropicus}:
Developmental Critical {Windows} and the Influence of
Temperature, Salinity, and Oxygen Availability",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "5",
day = "12",
month = feb,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010005",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/5",
abstract = "Alterations in fish developmental trajectories occur
in response to genetic and environmental changes,
especially during sensitive periods of development
(critical windows). Embryos and larvae of
\bioname{Atractosteus tropicus} were used as a model to
study fish survival, growth, and development as a
function of temperature (28 \degree C control, 33
\degree C, and 36 \degree C), salinity (0.0 ppt
control, 4.0 ppt, and 6.0 ppt), and air saturation
(control $ \approx $95\% air saturation, hypoxia $
\approx $30\% air saturation, and hyperoxia $ \approx
$117\% air saturation) during three developmental
periods: (1) fertilization to hatch, (2) day 1 to day 6
post hatch (dph), and (3) 7 to 12 dph. Elevated
temperature, hypoxia, and hyperoxia decreased survival
during incubation, and salinity at 2 and 3 dph. Growth
increased in embryos incubated at elevated temperature,
at higher salinity, and in hyperoxia but decreased in
hypoxia. Changes in development occurred as alterations
in the timing of hatching, yolk depletion, acceptance
of exogenous feeding, free swimming, and snout shape
change, especially at high temperature and hypoxia. Our
results suggest identifiable critical windows of
development in the early ontogeny of A. tropicus and
contribute to the knowledge of fish larval ecology and
the interactions of individuals $ \times $ stressors $
\times $ time of exposure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martens:2021:ECF,
author = "Kyle D. Martens and Jason Dunham",
title = "Evaluating Coexistence of Fish Species with Coastal
Cutthroat Trout in Low Order Streams of {Western Oregon
and Washington, USA}",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "4",
day = "30",
month = jan,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010004",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/4",
abstract = "When multiple species of fish coexist there are a host
of potential ways through which they may interact, yet
there is often a strong focus on studies of single
species without considering these interactions. For
example, many studies of forestry--stream interactions
in the Pacific Northwest have focused solely on the
most prevalent species: Coastal cutthroat trout. To
examine the potential for interactions of other fishes
with coastal cutthroat trout, we conducted an analysis
of 281 sites in low order streams located on
Washington's Olympic Peninsula and along the central
Oregon coast. Coastal cutthroat trout and juvenile coho
salmon were the most commonly found salmonid species
within these streams and exhibited positive
associations with each other for both presence and
density. Steelhead were negatively associated with the
presence of coastal cutthroat trout as well as with
coho salmon and sculpins (Cottidae). Coastal cutthroat
trout most frequently shared streams with juvenile coho
salmon. For densities of these co-occurring species,
associations between these two species were relatively
weak compared to the strong influences of physical
stream conditions (size and gradient), suggesting that
physical conditions may have more of an influence on
density than species interactions. Collectively, our
analysis, along with a review of findings from prior
field and laboratory studies, suggests that the net
effect of interactions between coastal cutthroat trout
and coho salmon do not appear to inhibit their presence
or densities in small streams along the Pacific
Northwest.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Office:2021:ARF,
author = "Fishes Editorial Office",
title = "Acknowledgment to Reviewers of Fishes in 2020",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "3",
day = "27",
month = jan,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010003",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/3",
abstract = "When multiple species of fish coexist there are a host
of potential ways through which they may interact, yet
there is often a strong focus on studies of single
species without considering these interactions. For
example, many studies of forestry--stream interactions
in the Pacific Northwest have focused solely on the
most prevalent species: Coastal cutthroat trout. To
examine the potential for interactions of other fishes
with coastal cutthroat trout, we conducted an analysis
of 281 sites in low order streams located on
Washington's Olympic Peninsula and along the central
Oregon coast. Coastal cutthroat trout and juvenile coho
salmon were the most commonly found salmonid species
within these streams and exhibited positive
associations with each other for both presence and
density. Steelhead were negatively associated with the
presence of coastal cutthroat trout as well as with
coho salmon and sculpins (Cottidae). Coastal cutthroat
trout most frequently shared streams with juvenile coho
salmon. For densities of these co-occurring species,
associations between these two species were relatively
weak compared to the strong influences of physical
stream conditions (size and gradient), suggesting that
physical conditions may have more of an influence on
density than species interactions. Collectively, our
analysis, along with a review of findings from prior
field and laboratory studies, suggests that the net
effect of interactions between coastal cutthroat trout
and coho salmon do not appear to inhibit their presence
or densities in small streams along the Pacific
Northwest.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Young:2021:ASL,
author = "Bradley Young and B. J. Allaire and Stephen Smith",
title = "Achieving Sea Lamprey Control in {Lake Champlain}",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "2",
day = "26",
month = jan,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010002",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/2",
abstract = "The control of parasitic sea lamprey in Lake Champlain
has been a necessary component of its fishery
restoration and recovery goals for 30 years. While
adopting the approach of the larger and established sea
lamprey control program of the Laurentian Great Lakes,
local differences emerged that shifted management focus
and effort as the program evolved. Increased investment
in lamprey assessment and monitoring revealed
under-estimations of population density and
distribution in the basin, where insufficient control
efforts went unnoticed. As control efforts improved in
response to a better understanding of the population,
the effects of lamprey on the fishery lessened. A
long-term evaluation of fishery responses when lamprey
control was started, interrupted, delayed, and enhanced
provided evidence of a recurring relationship between
the level of control effort applied and the measured
suppression of the parasitic sea lamprey population.
Changes in levels of control efforts over time showed
repeatedly that measurable suppression of the parasitic
population required effective control of 80\% of the
known larval population. Understanding the importance
of assessment and monitoring and the relationship
between control effort and population suppression has
led to recognition that a comprehensive, not
incremental, approach is needed to achieve effective
control of sea lamprey in Lake Champlain.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Flinn:2021:TGM,
author = "Shane A. Flinn and Stephen R. Midway",
title = "Trends in Growth Modeling in Fisheries Science",
journal = j-FISHES,
volume = "6",
number = "1",
pages = "1",
day = "19",
month = jan,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/1/1",
abstract = "Growth models estimate life history parameters (e.g.,
growth rates and asymptotic size) that are used in the
management of fisheries stocks. Traditionally in
fisheries science, it was common to fit one growth
model---the von Bertalanffy growth model---to
size-at-age data. However, in recent years, fisheries
science has seen an increase in the number of growth
models available and the evaluation of multiple growth
models for a given species or study. We reviewed n =
196 peer-reviewed age and growth studies and n = 50
NOAA (National Oceanic and Atmospheric Administration)
regional stock assessments to examine trends in the use
of growth models and model selection in fisheries over
time. Our results indicate that the total number of age
and growth studies increased annually since 1988 with a
slight proportional increase in the use of multi-model
frameworks. Information theoretic approaches are
replacing goodness-of-fit and a priori model selection
in fisheries studies; however, this trend is not
reflected in NOAA stock assessments, which almost
exclusively rely on the von Bertalanffy growth model.
Covariates such as system (e.g., marine or fresh),
location of study, diet, family, maximum age, and range
of age data used in model fitting did not contribute to
which model was ultimately the best fitting, suggesting
that there are no large-scale patterns of specific
growth models being applied to species with common life
histories or other attributes. Given the importance and
ubiquity of growth modeling to fisheries science, a
historical and contemporary understanding of the
practice is critical to evaluate improvements that have
been made and future challenges.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Azeiteiro:2021:DTA,
author = "Ulisses M. Azeiteiro and M{\'a}rio J. Pereira and
Amadeu M. V. M. Soares and Heitor O. Braga and Fernando
Morgado and Magda C. Sousa and Jo{\~a}o M. Dias and
Carlos Antunes",
title = "Dynamics of Two Anadromous Species in a Dam
Intersected River: Analysis of Two 100-Year Datasets",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "21",
day = "05",
month = jun,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020021",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/21",
abstract = "Long-term time-series datasets are key for assessing
the population dynamics of fish species with economic
interest. This study examines two 100-year datasets for
sea lamprey \bioname{Petromyzon marinus} and allis shad
\bioname{Alosa alosa} from the Minho River. This basin
on the Iberian Peninsula is home to one of the largest
populations at the southern distribution limit of these
critically endangered anadromous fish species. Besides
assessing the importance of the environmental drivers
of fish capture data (temperature, salinity, upwelling,
precipitation, and climatic oscillation), this study
also assesses how dam construction affected these
populations using intervention analysis --- a
statistical tool to detect significant breakpoints in
time series data. Results showed contrasting trends
between sea lamprey and allis shad, with the number of
captured fish from the first progressively improving
from 1914 to 2017, and the latter recording a
significant decline over time. Although no significant
correlations were detected between fishing data and
environmental variables, some of the identified
breakpoints in the time series data matched the dates
when the major dams in Minho River were built. Other
historical activities associated with mining might
explain the notable changes detected in the trends,
while issues associated with illegal, unreported, and
unregulated capture data are also discussed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cao:2021:EAA,
author = "Jie Cao and Qi Wang and Jun Mei and Jing Xie",
title = "Effect of 3-Aminobenzoic Acid Ethyl Ester
Methanesulfonate ({MS-222}) on Quality of Marine
Cultured Turbot (\bioname{Scophthalmus maximus}) during
Simulated Transport in Water",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "20",
day = "19",
month = may,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020020",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/20",
abstract = "This study evaluated the effect of different
concentrations (20, 40 and 60 mg/L) of 3-aminobenzoic
acid ethyl ester methanesulfonate (MS-222) on the
quality changes in turbot during simulated transport in
water. The results showed that the ammonia nitrogen
content in the transportation water of each sample
increased significantly, and the dissolved oxygen level
decreased. The dissolved oxygen content in
MS-222-treated samples was higher than that of control
group (CK) samples. For turbot flesh quality, simulated
transport in water led to a decrease in moisture, fat
and protein contents in all samples. The MS-222-treated
turbot samples showed higher pH values, glycogen
contents, springiness and chewiness values and lower
lactic acid contents comparing with the CK samples
during simulated transport in water. In addition, the
fresh and bitter amino acids in the muscle of turbot
increased in each treatment group compared to the
non-transported fish at the end of the simulated
transport. The results showed that MS-222 treatment
could retard the turbot transport stress and improve
the quality of turbot during simulated transport in
water.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ly:2021:EDD,
author = "Khanh Van Ly and David Kamau Murungu and Dung Phuong
Nguyen and Ngoc Anh Thi Nguyen",
title = "Effects of Different Densities of Sea Grape
\bioname{Caulerpa lentillifera} on Water Quality,
Growth and Survival of the Whiteleg Shrimp
\bioname{Litopenaeus vannamei} in Polyculture System",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "19",
day = "05",
month = may,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020019",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/19",
abstract = "The integrated aquaculture-seaweed system has been
identified as a bio-mitigation strategy to overcome
environmental damage, improve the efficiency of
nutrient use, maintain good water quality, and ensure
the system's sustainability. This study was conducted
to determine the appropriate density of sea grape
(Caulerpa lentillifera) in polyculture with whiteleg
shrimp (\bioname{Litopenaeus vannamei}) in the same
culture tank. Five treatments were randomly designed in
triplicate tanks where shrimp was monocultured (without
sea grape) as a control treatment and four polyculture
treatments with different seaweed density levels (0.5,
1, 1.5, and 2 kg m \minus 3) for 56 days. The results
showed that polyculture of shrimp and sea grape
significantly reduced the concentrations of total
ammonia nitrogen (TAN), nitrite (NO 2 \minus), nitrate
(NO 3 \minus), and phosphate (PO 4 3\minus) in the
rearing tanks and significantly improved (p {$<$} 0.05)
the growth rate (6.67--6.76\% day \minus 1), survival
(73.3--78.5\%), and production of shrimp (3.44--3.87 kg
m \minus 3) compared to monoculture (6.24\% day \minus
1, 54.8\%, and 2.02 kg m \minus 3, respectively).
Applying shrimp and sea grape polyculture at a density
of 1 kg m \minus 3 provided a relatively better shrimp
performance and feed conversion ratio than other
seaweed densities, although not significantly different
among polyculture treatments. The findings suggested
that sea grape could be used at densities of 0.5--2 kg
m \minus 3 in polyculture with whiteleg shrimp, of
which 1 kg m \minus 3 resulted in higher production and
feed efficiency.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Miyanishi:2021:ESS,
author = "Hiroshi Miyanishi and Katsuhisa Uchida",
title = "Establishment of a Simplified System to Evaluate
Salinity Preference and Validation of Behavioral
Salinity Selection in the \geoname{Japanese} Medaka,
\bioname{Oryzias latipes}",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "18",
day = "28",
month = apr,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/18",
abstract = "In fishes, it is necessary to select a salinity
environment suitable for survival. However, little is
known about the mechanisms regarding detection and
selection of salinity environments in fish. This study
involved the establishment of a simple aquarium system
in which fish can swim between freshwater (FW) and
seawater (SW) in a single tank. In this tank, the lower
level contained SW, the upper level contained FW, and
the FW and SW levels were clearly separated as
different salinity areas. Behavioral experiments of
salinity environment selection using this simplified
system to evaluate salinity preference showed that
FW-acclimated medakas preferred FW to SW. In contrast,
SW-acclimated medakas preferred SW to FW. These results
indicate that euryhaline medakas prefer the saline
habitats to which they are acclimated, when able to
select the salinity environment. We identified the
taste receptor type-2 and polycystic kidney disease
2-like 1 genes as possibly related to high-salinity
taste in medaka. The expression of these genes
increased at certain time points after SW challenges.
In this study, we established an aquarium system to
facilitate a simple experiment for salinity preference.
Our results suggest that the medaka is good model for
research related to seawater environment selection in
fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liang:2021:MCG,
author = "Xiao Liang and Ying Wan and Zhiyuan Shen and Yanmei
Liu and Dapeng Li and Li Li and Rong Tang and Xi
Zhang",
title = "Molecular Characterization of \gene{Hsp47} in Grass
Carp (\bioname{Ctenopharyngodon idella}) and Its
Correlation with {Type I} Collagen in Response to Fish
Aerobic Exercise",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "17",
day = "23",
month = apr,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020017",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/17",
abstract = "Heat shock protein 47 (Hsp47) is a collagen-specific
molecular chaperone that is indispensable for molecular
maturation of collagen. In this study, hsp47 and
hsp47-like cDNAs were cloned and characterized in grass
carp (\bioname{Ctenopharyngodon idella}). The cDNAs
were 1212 and 1218 base pairs long, respectively, and
included an open reading frame encoding 403 and 405
amino acids. The molecular phylogeny based on the
deduced amino acid sequences indicated that the correct
sequences of the hsp47 and hsp47-like cDNA were
obtained and the deduced proteins clustered distinctly
into teleost clades. Primary structure analysis and
characterization of Hsp47 and Hsp47-like shared the
basic structure and biofunctions of Hsp47 in
vertebrates. The spatial pattern of gene expression
revealed that hsp47 and hsp47-like were relatively
ubiquitous in different tissues and highly expressed in
heart and skin. The expression levels of hsp47 and
hsp47-like and type I collagen mRNAs varied similarly
in different tissues. Type I collagen content increased
significantly with the increase of water velocity in
the muscle of grass carp in response to aerobic
exercise. Among the gene expression changes of hsp47,
hsp47-like, col1a1 and col1a2 in muscle that occurred
in response to aerobic exercise, the change of type I
collagen was most strongly correlated with hsp47
expression. Additionally, col1a1 showed the highest
correlation with hsp47-like and col1a2 showed the
highest correlation with hsp47. These findings suggest
that grass carp Hsp47 and Hsp47-like are closely
related to type I collagen synthesis. This study
firstly suggests fish aerobic exercise can improve type
I collagen content and Hsp47 gene expression in muscle
of grass carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mukhtar:2021:SMP,
author = "Mutia Kamalia Mukhtar and Masita Dwi Mandini Manessa
and Supriatna Supriatna and Liya Tri Khikmawati",
title = "Spatial Modeling of Potential Lobster Harvest Grounds
in {Palabuhanratu Bay, West Java, Indonesia}",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "16",
day = "21",
month = apr,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/16",
abstract = "Palabuhanratu Bay is a location in the southern part
of Java Island with a high lobster population. Based on
field observation, the lobster population in
Palabuhanratu Bay is dominated by \bioname{Panulirus
homarus} (green sand lobster), \bioname{Panulirus
versicolor} (bamboo lobster), \bioname{Panulirus
penicillatus} (black lobster), and \bioname{Panulirus
ornatus} (pearl lobster). This study aimed to develop a
spatial model using satellite-derived data to predict
potential lobster harvest grounds in Palabuhanratu Bay.
The Earth observational satellite data used were
multispectral Landsat 8-SR imagery, and information
about chlorophyll-a, salinity, total suspended solids
(TSSs), sea surface temperature (SST), and distance
from the coastline was extracted. Multiple linear
regression was applied to build the prediction model,
which was validated using 10-fold cross-validation. The
result of the lobster harvest prediction model agreed
with the root-mean-square error (RMSE) and adjusted R 2
values of 0.326 and 0.708, respectively. The
distribution of lobsters was strong at the following
preferred ranges: chlorophyll-a: 1.1--1.7 mg/m 3;
salinity: 20.2--23.7 ppt; TSS: 40--56.4 mg/L; SST:
29.5--29.9 \degree C; and distance from the coastline:
500--4700 m. In this study, the habitats of four
species of lobsters and their relationships with
satellite-derived parameters were evaluated.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Espinosa-Ruiz:2021:WIC,
author = "Crist{\'o}bal Espinosa-Ru{\'\i}z and Mar{\'\i}a
{\'A}ngeles Esteban",
title = "Wound-Induced Changes in Antioxidant Enzyme Activities
in Skin Mucus and in Gene Expression in the Skin of
Gilthead Seabream (\bioname{Sparus aurata} {L.})",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "15",
day = "18",
month = apr,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020015",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/15",
abstract = "This study investigated the antioxidant enzyme
activities in the skin mucus of gilthead seabream
(\bioname{Sparus aurata} {L.}) at 3 and 7 days
post-wounding (dpw). The expression levels of the genes
that encode stress proteins (grp170, grp94, grp75, sod
and hsp70) and skin regeneration-related proteins (tf,
igf1, tgfb1, der1, apo1 and erdj3) in the skin also
were determined. Mucus and skin samples were obtained
from the left and right flanks of non-wounded and
wounded fish. In both flanks of the wounded fish,
catalase and glutathione reductase activities in the
skin mucus increased (p {$<$} 0.05) at 3 and 7 dpw (100
\pm 31\% and 111 \pm 25\%, respectively), whereas
superoxide dismutase activity increased (p {$<$} 0.05)
only at 7 dpw (135 \pm 15\%). The expression levels of
stress proteins in the skin of the wounded flank of the
wounded fish mainly increased at 7 dpw (grp170
increased to 288 \pm 85\%, grp94 to 502 \pm 143\%,
grp75 to 274 \pm 69\%, sod to 569 \pm 99\%, and hsp70
increased to 537 \pm 14\%) (p {$<$} 0.05). However, the
expression levels of the tissue regeneration-related
genes varied depending on the flank investigated, on
the experimental time, and on the gene studied. To the
best of our knowledge, this is the first work to
determine the effect of a wound in different skin parts
of the same fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Leese:2021:EWT,
author = "Joseph M. Leese and Julia McMahon and Joseph C.
Colosi",
title = "Effects of Wastewater Treatment Plant Effluent in a
Receiving Stream on Reproductive Behavior of Fathead
Minnows (\bioname{Pimephales promelas})",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "14",
day = "12",
month = apr,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020014",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/14",
abstract = "Wastewater treatment plant effluents contain a variety
of endocrine disrupting chemicals (EDCs), including
chemicals with estrogenic activity such as 17\beta
-estradiol (E2), 17\alpha -ethinyl estradiol (EE2), and
nonylphenols. These substances can affect both behavior
and physiology in vertebrate animals. To explore the
presence and effects of these EDCs in a natural
setting, juvenile and adult male fathead minnows,
\bioname{Pimephales promelas}, were held in cages
upstream and downstream of the effluent site of a
wastewater treatment plant for 21 days and subsequently
tested for changes in reproductive behaviors and
production of vitellogenin. Additionally, estrogenic
activity in the stream was measured using a yeast
bioassay. Estrogenicity was found to be significantly
higher downstream of the wastewater effluent when
compared to levels upstream. Vitellogenin levels did
not show a correlational pattern with levels of
estrogenicity in the water, but two measures of
reproductive behaviors occurred significantly less
often in downstream males than upstream males. This
suggests that a brief (three-week) exposure to stream
water containing wastewater treatment plant effluent
can bring about changes in reproductive behavior of
fish and that behavior may be more sensitive to low
levels of environmental endocrine disruptors than
vitellogenin production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ruban:2021:BCP,
author = "Dmitry A. Ruban and Vladimir A. Ermolaev",
title = "Black Caviar Perturbs Reflection of {Russian}
Geography: a Research Note of Aquaculture-Triggered
Place Naming Puzzle",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "13",
day = "06",
month = apr,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020013",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/13",
abstract = "Black caviar is often thought to be a typically
Russian luxurious food product. Recently, its
production has extended due to sturgeon aquaculture
development. The analysis of the geographical
affinities of the full-cycle companies and their
aquaculture-sourced black caviar implies that the
majority of them employs the Russian Caviar brand
and/or refers to the Russian traditions and history.
However, names, brands, and/or product positioning of
several companies tend to mention geographical objects
associated with the tradition places of black caviar
production (Astrakhan, the Caspian Sea, and the Volga
River), whereas these companies are located in the
other, sometimes remote places (with distances over
1000 km). Such marketing solutions of black caviar
producers perturb reflection of the Russian geography.
The geographical indication (place of origin) of this
fish product needs improvement, which is a task for
companies, state, and professional societies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lee:2021:MDC,
author = "Cheng-Linn Lee and Yuri Kominami and Hideki Ushio",
title = "Mechanism of Delayed Convulsion in Fish: the Actions
of Norepinephrine in Spinal Cord",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "12",
day = "31",
month = mar,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020012",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/12",
abstract = "Cranial spiking (CS) is among the most popular
slaughtering methods for delaying the rigor mortis
progress of fish muscles. However, it may cause a
convulsion (subsequently referred to as delayed
convulsion), which undermines the meat quality and
taste. This study aimed to elucidate the mechanism
underlying the delayed convulsion and examine its
influence on ATP consumption. Ten carps, nine tilapias,
ten rainbow trouts, two ayus, three greenling,
thirty-five red seabreams, two striped jack and two
stone flounders underwent CS around the medulla
oblongata area, which induced different delayed
convulsion profiles specific to each species. To
investigate the norepinephrine (NE) actions related to
delayed convulsion, 27 red seabreams, a representative
fish species that exhibits delayed convulsion, were
treated with a monoamine-depleting agent, reserpine, or
with a monoamine oxidase inhibitor, pargyline, two
hours before CS. Spinal cord destruction (SCD) was
employed to completely prevent spinal cord functions of
the fish in another group. Compared with the control
group (CS only), the reserpine, pargyline, and SCD
groups showed significantly inhibited delayed
convulsion and ATP consumption. This suggests that
delayed convulsion is the main ATP-consuming response.
Our findings suggest that delayed clonic convulsion in
red seabreams is associated with the rapid decrease in
spinal cord NE levels, which triggered the rebound
motor neuron hyperactivity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wenzel:2021:EDP,
author = "Lisa Carolina Wenzel and Sebastian Marcus Strauch and
Ep Eding and Francisco Xose Presas-Basalo and Berit
Wasenitz and Harry Wilhelm Palm",
title = "Effects of Dissolved Potassium on Growth Performance,
Body Composition, and Welfare of Juvenile
\geoname{African} Catfish (\bioname{Clarias
gariepinus})",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "11",
day = "28",
month = mar,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020011",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/11",
abstract = "Optimal crop production in aquaponics is influenced by
water pH and potassium concentrations. The addition of
potassium hydroxide (KOH) into the recirculating
aquaculture system (RAS) may benefit aquaponics by
increasing the water pH for better biofilter activity
and supplementing K for better plant growth and
quality. We investigated the growth, feed conversion,
body composition and welfare indicators of juvenile
African catfish (\bioname{Clarias gariepinus}) treated
with four concentrations of K (K0 = 2, K200 = 218, K400
= 418, and K600 = 671 mg L \minus 1). While growth,
feed conversion and final body composition were
unaffected, the feeding time and individual resting
significantly increased with increasing K +. The
swimming activity and agonistic behavior were reduced
significantly under increased concentrations of K +.
Leftover feed and the highest number of skin lesions
were observed under K600. We suggest that K +
concentrations between 200 and 400 mg L \minus 1 can
improve the welfare status of juvenile African catfish.
This enables the application of KOH in RAS to supply
alkalinity to achieve optimum nitrification at minimum
water exchange and improve the nutritional profile of
the process water with benefits for the welfare status
of African catfish and aquaponics plant production and
quality.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zielinski:2021:NSD,
author = "Daniel Patrick Zielinski and Peter W. Sorensen",
title = "Numeric Simulation Demonstrates That the Upstream
Movement of Invasive Bigheaded Carp Can Be Blocked at
Sets of \geoname{Mississippi River} Locks-and-Dams
Using a Combination of Optimized Spillway Gate
Operations, Lock Deterrents, and Carp Removal",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "10",
day = "26",
month = mar,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020010",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/10",
abstract = "Invasive bigheaded carp are advancing up the Upper
Mississippi River by passing through its locks-and-dams
(LDs). Although these structures already impede fish
passage, this role could be greatly enhanced by
modifying how their spillway gates operate, adding
deterrent systems to their locks, and removing carp.
This study examined this possibility using numeric
modeling and empirical data, which evaluated all three
options on an annual basis in both single LDs and pairs
under different river flow conditions. Over 100
scenarios were modeled. While all three approaches
showed promise, ranging from 8\% to 73\% reductions in
how many carp pass a single LD, when employed together
at pairs of LDs, upstream movement rates of invasive
carp could be reduced 98--99\% from current levels.
Although modifying spillway gate operation is the least
expensive option, its efficacy drops at high flows, so
lock deterrents and/or removal using fishing/trapping
are required to move towards complete blockage.
Improved deterrent efficacy could also offset the need
for more efficient removal. This model could help
prioritize research and management actions for
containing carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Coburn:2021:APC,
author = "Jessica Coburn and M. Scott Wells and Nicholas B. D.
Phelps and T. Gibson Gaylord and Deborah A. Samac",
title = "Acceptance of a Protein Concentrate from Alfalfa
(\bioname{Medicago sativa}) by Yellow Perch
(\bioname{Perca flavescens}) Fed a Formulated Diet",
journal = j-FISHES,
volume = "6",
number = "2",
pages = "9",
day = "25",
month = mar,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6020009",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:26 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/2/9",
abstract = "The majority of plant proteins used in aquatic feeds
are derived from seed meals, which may contain
antinutritional factors. Protein concentrates from
plant foliage have received less attention in fish
feeding trials. Alfalfa protein concentrate (APC) is
derived from fresh alfalfa foliage that contains
approximately 52\% protein and is low in fiber. A
feeding trial was done to assess growth and feed
efficiency responses of yellow perch (\bioname{Perca
flavescens}) fed a formulated diet with 180 g/kg APC
replacing all fishmeal compared to a control
isonitrogenous diet with fishmeal. Yellow perch
accepted the APC diet but gained weight at a lower
specific growth rate (\minus 0.07\% per day) and had an
elevated feed conversion ratio (+0.32 g feed/g growth)
than fish on the control diet containing fishmeal.
There was no impact on survivorship or condition nor
differences in fillet yield or composition in fish on
the diet with APC compared to the control fishmeal
diet. These findings indicate that although replacing
fishmeal with APC in a perch diet resulted in slower
growth rates, the APC was accepted and has promise as a
sustainable protein in aquatic feeds.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Guo:2021:CAN,
author = "Zhansheng Guo and Zhen Wang and Xuguang Hou",
title = "Comparative Analysis of the {nrDNA} Repeat Unit of
\geoname{Manila} Clam \bioname{Ruditapes philippinarum}
and Quahog \bioname{Mercenaria mercenaria}",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "42",
day = "17",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030042",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/42",
abstract = "\bioname{Ruditapes philippinarum} and
\bioname{Mercenaria mercenaria} are economically
important bivalve species. The complete ribosomal DNA
(rDNA) unit sequences of R. philippinarum and M.
mercenaria, with as-sembled rDNA unit lengths of 12,910
and 12,100 bp, respectively, were obtained in this
study for the first time. The rDNA unit structural
organisation was similar to that in other eukaryotes,
in-cluding the following elements in order: 18S
rRNA-internal transcribed spacer 1 (ITS1); 5.8S
rRNA-ITS2-28S rRNA-intergenic spacer (IGS) (3\prime
external transcribed spacer (ETS); non-transcribed
spacer (NTS)-5\prime ETS). The genetic differences
between R. philippinarum and M. mercenaria were mainly
attributable to non-coding regions (ITS1, ITS2 and
IGS), especially the IGS region. The boundaries of
putative 3\prime ETS, NTS and 5\prime ETS were
confirmed. Seven and three sub-repeat fragments were
found in R. philippinarum and M. mercenaria,
respectively. These frag-ments ranged from 4 to 154 bp
in length, and were located at the NTS and 5\prime ETS
regions. Five and six cytosine--guanine (CpG) islands
were detected in R. philippinarum and M. mercenaria,
respec-tively, and these covered 85.58\% and 79.29\% of
the entire IGS sequence, respectively. The
phylo-genetic tree was constructed based on Veneridae
ITS and 18S rRNA sequences using the maxi-mum
likelihood (ML) method. The ML tree based on ITS
revealed that species within the same genus clearly
clustered together with relatively high supporting
values, and all the genera were recovered as
monophyletic. The phylogenetic analyses using 18S rRNA
provided a weaker phy-logenetic signal than ITS.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wei:2021:EEF,
author = "Xinyi Wei and Qiuguang Hu and Jintao Ma",
title = "Evaluation on the Effect of Fishery Insurance Policy:
Evidence Based on Text Mining",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "41",
day = "13",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030041",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/41",
abstract = "As a quasi-public product, fishery insurance has
become an important starting point for the construction
of the modern fishery industry chain, supply chain and
value chain risk management mechanism. We used visual
data processing methods and text mining technology to
screen policy samples. We then built a fishery
insurance policy evaluation system based on the Policy
Modeling Consistency (PMC) index model. We combined the
PMC index score and PMC surface to quantitatively
analyze the policy samples. This paper has four
important findings: (1) After three adjustments and
developments, the fishery insurance policy has grown in
terms of initial attention, changes, and development
and gradually matured. (2) A gap exists between the
content of the fishing insurance policy text and the
actual demand. The scoring results of the policy
samples are concentrated in the acceptable range, the
policy effects are not satisfactory, and the
formulation of fishery insurance policies has weak
links that need to be improved. (3) The consistency and
effectiveness of fishery insurance policies have
developed simultaneously with fishery insurance
research, and the practical effects of high-quality
fishery insurance policies are conducive to the
development of theoretical research. (4) The policy
text of fishery insurance has major problems, such as
missing joint force of issuing institutions, low
professionalism of the text, inadequate subdivision
guidance of fishery insurance, weak social
effectiveness, high dependence on financial subsidies,
lack of incentive sustainability and corresponding laws
and regulations and reduction in policy feasibility
among others. Considering the above issues, this paper
puts forward relevant policy optimization paths and
safeguard measures on the basis of giving priority to
greater absolute value.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chai:2021:IFS,
author = "Panfeng Chai and Qiuguang Hu and Xinyi Wei",
title = "Influence of Fishery Subsidies on Fishing: Empirical
Test Based on {China}'s Provincial Panel Data",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "40",
day = "13",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030040",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/40",
abstract = "Controversies surrounding fishery subsidy policies are
widespread. Many stakeholders believe that fishery
subsidies play an important role in ensuring the
livelihood of fishermen. At the same time, fishery
subsidies pose a threat to the stock of fishery
resources and affect the sustainable development of
fisheries. Based on the panel data of 30 provinces,
municipalities, and autonomous regions in China from
2007 to 2017, the article empirically examines the
influence of fishery subsidies on fishing. The results
of the study show that China\prime s fishery subsidies
are negatively correlated with fishing. On average, for
each 1\% increase in fishery subsidies, fishing will
decrease by 2.9\%. That is to say, in general, fishery
subsidies are conducive to the sustainable development
of fisheries. The results of heterogeneity analysis
based on geographic location and economic development
level show that fishery subsidies do not have a
palpable negative influence on fishing in coastal areas
or developed regions, whereas have an obvious adverse
influence on fishing in inland areas or underdeveloped
regions. The deterioration of the fishing environment
and the natural environment explains the occurrence of
this differentiation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chu:2021:SLP,
author = "Yuanming Chu and Mingtang Tan and Zhengkai Yi and
Zhaoyang Ding and Dazhang Yang and Jing Xie",
title = "Shelf-Life Prediction of Glazed Large Yellow Croaker
(\bioname{Pseudosciaena crocea}) during Frozen Storage
Based on {Arrhenius} Model and Long-Short-Term Memory
Neural Networks Model",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "39",
day = "10",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030039",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/39",
abstract = "In this study, the changes in centrifugal loss, TVB-N,
K-value, whiteness and sensory evaluation of glazed
large yellow croaker were analyzed at \minus 10, \minus
20, \minus 30 and \minus 40 \degree C storage. The
Arrhenius prediction model and long-short-term memory
neural networks (LSTM-NN) prediction model were
developed to predict the shelf-life of the glazed large
yellow croaker. The results showed that the quality of
glazed large yellow croaker gradually decreased with
the extension of frozen storage time, and the decrease
in quality slowed down at lower temperatures. Both the
Arrhenius model and the LSTM-NN prediction model were
good tools for predicting the shelf-life of glazed
large yellow croaker. However, for the relative error,
the prediction accuracy of LSTM-NN (with a mean value
of 7.78\%) was higher than that of Arrhenius model
(with a mean value of 11.90\%). Moreover, the LSTM-NN
model had a more intelligent, convenient and fast data
processing capability, so the new LSTM-NN model
provided a better choice for predicting the shelf-life
of glazed large yellow croaker.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Abo-Taleb:2021:GPF,
author = "Hamdy A. Abo-Taleb and Mohamed M. M. El-feky and Ahmad
M. Azab and Mohamed M. Mabrouk and Mohamed A. Elokaby
and Mohamed Ashour and Abdallah Tageldein Mansour and
Othman F. Abdelzaher and Khamael M. Abualnaja and Ahmed
E. Sallam",
title = "Growth Performance, Feed Utilization, Gut Integrity,
and Economic Revenue of Grey Mullet, \bioname{Mugil
cephalus}, Fed an Increasing Level of Dried Zooplankton
Biomass Meal as Fishmeal Substitutions",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "38",
day = "10",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030038",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/38",
abstract = "Fishmeal is the most expensive feedstuff in the
aquafeed and one of the most environmentally limiting
factor of aquaculture development. Therefore, the
search for alternative protein sources is a continuous
process. The present feeding trial was conducted to
evaluate the effects of replacing fishmeal with
zooplankton biomass meal (ZBM) on the growth
performance, nutrient utilization, intestine, and liver
histological changes of grey mullet, \bioname{Mugil
cephalus} (initial weight of 0.10 \pm 0.01 g). Five
isoproteic (35\% crude protein) and isolipidic (8\%
crude lipid) diets were formulated as the control diet
(Z 0) and the other four diets (Z 25, Z 50, Z 75, and Z
100), where 25\%, 50\%, 75\%, and 100\% of fishmeal was
replaced by ZBM, respectively. After 60 days of
feeding, the final weight, weight gain, and daily
growth index of the grey mullet fed the Z 100 diet were
higher than those fed the control diet (p {$<$} 0.05).
In addition, the better values of feed conversion
ratio, protein efficiency ratio and lipid efficiency
ratio were recorded in the fish fed with the Z 100
diet. Additionally, the intestinal villus length,
crypts depth, and muscle thickness were significantly
improved with ZBM inclusion (p {$<$} 0.05). Meanwhile,
there were no histopathological changes observed on the
liver when compared with the control group. From the
economic point of view, dietary substitution of
fishmeal by ZBM (Z 100) reduced the cost of diet
formulation by 18\% and the price per kg weight gain by
about 40\%. Overall, according to the findings of this
study, substituting fishmeal with ZBM up to 100\% could
improve growth performance, feed utilization, gut
health status, and profit ability of rearing M.
cephalus juveniles.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Haguenauer:2021:DHC,
author = "Anne Haguenauer and Fr{\'e}d{\'e}ric Zuberer and
Gilles Siu and Daphne Cortese and Ricardo Beldade and
Suzanne C. Mills",
title = "Deep Heat: a Comparison of Water Temperature, Anemone
Bleaching, Anemonefish Density and Reproduction between
Shallow and Mesophotic Reefs",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "37",
day = "09",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030037",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/37",
abstract = "French Polynesia is experiencing increasing coral
bleaching events in shallow waters triggered by thermal
anomalies and marine heatwaves linked to climate
change, a trend that is replicated worldwide. As sea
surface thermal anomalies are assumed to lessen with
depth, mesophotic deep reefs have been hypothesized to
act as refuges from anthropogenic and natural
disturbances, the \lq deep reef refugia hypothesis'
(DRRH). However, evidence supporting the DRRH is either
inconclusive or conflicting. We address this by
investigating four assumptions of the DRRH focusing on
the symbiotic association between anemones and
anemonefish. First, we compare long-term temperature
conditions between shallow (8 m) and mesophotic sites
(50 m) on the island of Moorea from 2011--2020. Second,
we compare the densities of the orange-fin anemonefish,
\bioname{Amphiprion chrysopterus} between shallow and
mesophotic (down to 60 m) reefs across three
archipelagos in French Polynesia. Finally, we compare
the percentage of anemone bleaching, as well as
anemonefish reproduction, between shallow and
mesophotic reefs. We found that the water column was
well mixed in the cooler austral winter months with
only a 0.19 \degree C difference in temperature between
depths, but in the warmer summer months mixing was
reduced resulting in a 0.71--1.03 \degree C temperature
difference. However, during thermal anomalies, despite
a time lag in warm surface waters reaching mesophotic
reefs, there was ultimately a 1.0 \degree C increase in
water temperature at both 8 and 50 m, pushing
temperatures over bleaching thresholds at both depths.
As such, anemone bleaching was observed in mesophotic
reefs during these thermal anomalies, but was buffered
compared to the percentage of bleaching in shallower
waters, which was nearly five times greater. Our
large-scale sampling across French Polynesia found
orange-fin anemonefish, A. chrysopterus, in mesophotic
zones in two high islands and one atoll across two
archipelagos, extending its bathymetric limit to 60 m;
however, orange-fin anemonefish densities were either
similar to, or 25--92 times lower than in shallower
zones. Three spawning events were observed at 50 m,
which occurred at a similar frequency to spawning on
shallower reefs at the same date. Our findings of
thermal anomalies and bleaching in mesophotic reefs,
coupled with mainly lower densities of anemonefish in
mesophotic populations, suggest that mesophotic reefs
show only a limited ability to provide refugia from
anthropogenic and natural disturbances.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huang:2021:SDP,
author = "Yongyu Huang and Lili Zhang and Hui Ge and Guodong
Wang and Shiyu Huang and Zhangwu Yang",
title = "{SNP} Development in \bioname{Penaeus vannamei} via
Next-Generation Sequencing and {DNA} Pool Sequencing",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "36",
day = "07",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030036",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/36",
abstract = "Next-generation sequencing and pool sequencing have
been widely used in SNP (single-nucleotide
polymorphism) detection and population genetics
research; however, there are few reports on SNPs
related to the growth of \bioname{Penaeus vannamei}.
The purpose of this study was to call SNPs from
rapid-growing (RG) and slow-growing (SG) individuals'
transcriptomes and use DNA pool sequencing to assess
the reliability of SNPs. Two parameters were applied to
detect SNPs. One parameter was the p -values generated
using Fisher's exact test, which were used to calculate
the significance of allele frequency differences
between RG and SG. The other one was the AFI (minor
allele frequency imbalance), which was defined to
highlight the fold changes in MAF (minor allele
frequency) values between RG and SG. There were 216,015
hypothetical SNPs, which were obtained based on the
transcriptome data. Finally, 104 high-quality SNPs and
96,819 low-quality SNPs were predicted. Then, 18
high-quality SNPs and 17 low-quality SNPs were selected
to assess the reliability of the detection process.
Here, 72.22\% (13/18) accuracy was achieved for
high-quality SNPs, while only 52.94\% (9/17) accuracy
was achieved for low-quality SNPs. These SNPs enrich
the data for population genetics studies of P. vannamei
and may play a role in the development of SNP markers
for future breeding studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Curiel-Bernal:2021:UOR,
author = "Marcelo V. Curiel-Bernal and E. Alberto
Arag{\'o}n-Noriega and Miguel {\'A}. Cisneros-Mata and
Laura S{\'a}nchez-Velasco and S. Patricia A.
Jim{\'e}nez-Rosenberg and Alejandro Par{\'e}s-Sierra",
title = "Using Observed Residual Error Structure Yields the
Best Estimates of Individual Growth Parameters",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "35",
day = "02",
month = sep,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030035",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/35",
abstract = "Obtaining the best possible estimates of individual
growth parameters is essential in studies of
physiology, fisheries management, and conservation of
natural resources since growth is a key component of
population dynamics. In the present work, we use data
of an endangered fish species to demonstrate the
importance of selecting the right data error structure
when fitting growth models in multimodel inference. The
totoaba (\bioname{Totoaba macdonaldi}) is a fish
species endemic to the Gulf of California increasingly
studied in recent times due to a perceived threat of
extinction. Previous works estimated individual growth
using the von Bertalanffy model assuming a constant
variance of length-at-age. Here, we reanalyze the same
data under five different variance assumptions to fit
the von Bertalanffy and Gompertz models. We found
consistent significant differences between the constant
and nonconstant error structure scenarios and provide
an example of the consequences using the growth
performance index \varphi \prime to show how using the
wrong error structure can produce growth parameter
values that can lead to biased conclusions. Based on
these results, for totoaba and other related species,
we recommend using the observed error structure to
obtain the individual growth parameters.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Choi:2021:EMT,
author = "Soo-Cheol Choi and In-Ah Lee",
title = "Effect of {MMP\slash TIMP} Balancing of
\bioname{Cynoglossus semilaevis} Shell Extracts on Skin
Protection",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "34",
day = "24",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030034",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/34",
abstract = "Cynoglossus semilaevis shell is a by-product of the
Cynoglossus semilaevis, a species of fish mainly
distributed along the west coast of Korea. As its skin
is very tough and difficult to process, it is not
useful as food. For this reason, most of it is
discarded except for a small amount that is used as
feed, which results in environmental pollution.
Considering this, there is a need for research on the
development of functional materials using Cynoglossus
semilaevis shell. This study focused on the mechanism
of in vitro expression function of \bioname{Cynoglossus
semilaevis} shell extract (CSE) for skin tissue in
human dermal fibroblasts that induced or did not induce
wrinkles by UV-B irradiation and aims to use it as a
functional material for human skin beauty or wrinkle
improvement through extraction and purification.
According to the ELISA results using human dermal
fibroblast cells, CSE reduced MMP-1 and elastase
activity by up to 21.89\% and 12.04\%, respectively, in
a concentration-dependent manner, and increased PIP
synthesis by up to 62.24\% in a concentration-dependent
manner. The RT-PCR test results using mRNA showed the
MMP-1, 2, and 3 expression levels were suppressed in
the CSE-treated group compared to the UVB-induced group
and caused a concentration-dependent increase in TIMP-1
in the CSE-treat group. These results suggest that CSE
can maintain and improve skin tissue conditions through
MMP/TIMP balancing in human dermal fibroblast cell
lines and indicate its potential as a functional
material for improving skin diseases and suppressing
photo-aging.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Faruque:2021:CSA,
author = "Hasan Faruque and Hiroyuki Matsuda",
title = "Conservative Scoring Approach in Productivity
Susceptibility Analysis Leads to an Overestimation of
Vulnerability: a Study from the Hilsa Gillnet Bycatch
Stocks of \geoname{Bangladesh}",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "33",
day = "24",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030033",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/33",
abstract = "Despite different approaches used to assign the risk
scores for missing information in productivity
susceptibility analysis (PSA) --- a widely used
semi-quantitative risk assessment tool for target and
non-target fisheries stocks --- for the selected
attributes of a given species, no formal comparison has
been made between scoring approaches in terms of how
well they can predict species vulnerability. The
present study evaluated the PSA findings of 21 bycatch
stocks of the Hilsa (\bioname{Tenualosa ilisha})
gillnet fishery of Bangladesh using two different
scoring approaches (the conservative scoring approach,
CSA; and the alternative scoring approach, ASA) to
determine the most reliable approach to minimize false
estimates of species vulnerability. Our analysis
revealed that the V scores increased by 0.0\minus 0.20
with a mean value of 0.09 for 21 selected bycatches
when CSA was applied. The inconsistency between the
vulnerability (V)-score-suggested fishing status (V \le
1.8 = underfishing, V $>$ 1.8 = overfishing) and the
fishing status defined by exploitation rate (E $>$ 0.5
= overfishing, E {$<$} 0.5 = underfishing) were 38.1\%
and 19.0\% under CSA and ASA, respectively. Likewise,
the consistency between the V -score-suggested fishing
status and fishers' perceived catch trends was found to
be higher when using ASA than when using CSA. Our
analysis suggests that CSA could overestimate species
vulnerability. Therefore, ASA is more reliable than CSA
in PSA, which may increase the confidence of fisheries
stakeholders in PSA.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{daSilva:2021:VAA,
author = "Elisia Gomes da Silva and Guerino Bandeira Junior and
Juliana Felipetto Cargnelutti and Roberto Christ Vianna
Santos and Andr{\'e} G{\"u}ndel and Bernardo
Baldisserotto",
title = "In Vitro Antimicrobial and Antibiofilm Activity of
{S}-(-)-Limonene and {R}-(+)-Limonene against Fish
Bacteria",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "32",
day = "19",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030032",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/32",
abstract = "Brazilian fish farming goes together with the
emergence of numerous bacterial diseases, with
\bioname{Aeromonas hydrophila} being the main bacterial
pathogen. As a consequence, antimicrobials are
excessively used. Considering that antimicrobials are
relatively stable and nonbiodegradable, medicinal
plants and their phytochemicals have been used as
alternative treatments of bacteriosis in fish farming.
Limonene is a monoterpene available in two enantiomers:
R-(+)-limonene and S-(-)-limonene. This study analyzed
the antibacterial activity of the phytochemicals
S-(-)-limonene and R-(+)-limonene against some bacteria
isolated from silver catfish (\bioname{Rhamdia
quelen}). Furthermore, by means of spectrophotometry
and atomic force microscopy, we also investigated the
combination therapy of phytochemicals with
antimicrobials and their activity in terms of
inhibiting biofilm formation. Six clinical isolates and
a standard strain were selected for antimicrobial
activity testing. Biofilm formation was tested in
96-well plates and nylon cubes. The most sensitive of
the strains tested was the A. hydrophila strain (MF
372510). S-(-)-limonene and R-(+)-limonene had high
minimum inhibitory concentrations; however, they
strongly inhibited A. hydrophila biofilm formation.
R-(+)-limonene and S-(-)-limonene had an additive
effect when combined with florfenicol and an
antagonistic effect with oxytetracycline. In general,
the phytochemicals tested showed strong antibiofilm
activity against A. hydrophila, and when in combination
therapy with florfenicol, they showed an additive
effect against the treatment of \bioname{A.
hydrophila}.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Aride:2021:DGE,
author = "Paulo Henrique R. Aride and Maria Fernanda S. Gomes
and Darlan G. Azevedo and Gilson R. Sangali and Ana
Cl{\'a}udia F. Silva and Henrique D. Lavander and
Andr{\'e} B. Souza and Marcelo F. Polese and Douglas C.
Mattos and Lucas A. Bassul and Leonardo D. Cardoso and
Adriano T. Oliveira and Caterina Faggio",
title = "Dusky Grouper \bioname{Epinephelus marginatus} Growth
and Survival When Exposed to Different Photoperiods",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "31",
day = "12",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030031",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/31",
abstract = "Photoperiod is considered an environmental factor that
influences reproduction and the growth processes of
fish throughout the year. In the present study, we
subjected dusky grouper \bioname{Epinephelus
marginatus} to different photoperiods in order to
evaluate growth and survival. Juvenile dusky grouper
were randomly distributed in twelve 100 L tanks
containing four fish per aquarium. The fish were
exposed to continuous light, normal photoperiod or
continuous darkness for 50 days. Fish were fed 3\% of
their total biomass, twice a day, and the diet
consisted of ground commercial dry pellets (42\% crude
protein, 12\% humidity, 9\% ether extract, 15\% mineral
matter, 4\% crude fiber, 3.5\% calcium and 3\% vitamin
C, in accordance with the manufacturer's instructions).
There was no mortality during the experiment. After 50
days, the best performance was found for exposure to
continuous light (24 h artificial light). The final
weight of the fish reared under continuous light was
significantly higher than that of the fish exposed to
continuous darkness. The specific growth rate of the
fish exposed to continuous light was significantly
higher than that of the fish exposed to the natural
photoperiod and to continuous darkness. Modifications
to the photoperiod can be a key factor in increasing
the efficacy of current production and the improvement
of current aquaculture protocols.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nguyen:2021:BPF,
author = "Van Bon Nguyen and San-Lang Wang and Anh Dzung Nguyen
and Tu Quy Phan and Kuaanan Techato and Siriporn
Pradit",
title = "Bioproduction of Prodigiosin from Fishery Processing
Waste Shrimp Heads and Evaluation of Its Potential
Bioactivities",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "30",
day = "11",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030030",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/30",
abstract = "The aim of this work was to reuse a fish processing
waste, shrimp head powder (SHP), for the production of
prodigiosin (PG) via microbial technology and to assess
its potential bioactivities. PG was produced in a 12
L-bioreactor system, and the highest PG productivity of
6310 mg L \minus 1 was achieved when \bioname{Serratia
marcescens} CC17 was used for fermentation in a novel
designed medium (6.75 L) containing 1.5\% C/N source
(SHP/casein = 9/1), 0.02\% K 2 SO 4, ans 0.025\% Ca 3
(PO 4) 2, with initial pH 7.0, and fermentation was
performed at 28 \degree C for 8 h. The purified PG
showed moderate antioxidants, efficient anti-NO
(anti-nitric oxide), and acetylcholinesterase (AChE)
inhibitory activities. In a docking study, PG showed
better binding energy scores (\minus 12.3 kcal/mol) and
more interactions (6 linkages) with several prominent
amino acids in the biding sites on AChE that were
superior to those of Berberine chloride (\minus 10.8
kcal/mol and one linkage). Notably, this is the first
investigation using shrimp heads for the mass
bioproduction of PG with high productivity, and Ca 3
(PO 4) 2 salt was also newly found to significantly
enhance PG production by S. marcescens. This study also
provided available data on the anti-NO and anti-AChE
effects of PG, especially from the docking simulation
PG towards AChE that was described for the first time
in this study. The above results suggest that SHP is a
good material for the cost-effective bioproduction of
PG, which is a potential candidate for anti-NO and
anti-Alzheimer drugs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esteban:2021:FRF,
author = "Maria Angeles Esteban and Bernardo Baldisserotto and
Eric Hallerman",
title = "{{\booktitle{Fishes}}} Receives Its First Impact
Factor",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "29",
day = "11",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030029",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/29",
abstract = "The aim of this work was to reuse a fish processing
waste, shrimp head powder (SHP), for the production of
prodigiosin (PG) via microbial technology and to assess
its potential bioactivities. PG was produced in a 12
L-bioreactor system, and the highest PG productivity of
6310 mg L \minus 1 was achieved when \bioname{Serratia
marcescens} CC17 was used for fermentation in a novel
designed medium (6.75 L) containing 1.5\% C/N source
(SHP/casein = 9/1), 0.02\% K 2 SO 4, ans 0.025\% Ca 3
(PO 4) 2, with initial pH 7.0, and fermentation was
performed at 28 \degree C for 8 h. The purified PG
showed moderate antioxidants, efficient anti-NO
(anti-nitric oxide), and acetylcholinesterase (AChE)
inhibitory activities. In a docking study, PG showed
better binding energy scores (\minus 12.3 kcal/mol) and
more interactions (6 linkages) with several prominent
amino acids in the biding sites on AChE that were
superior to those of Berberine chloride (\minus 10.8
kcal/mol and one linkage). Notably, this is the first
investigation using shrimp heads for the mass
bioproduction of PG with high productivity, and Ca 3
(PO 4) 2 salt was also newly found to significantly
enhance PG production by S. marcescens. This study also
provided available data on the anti-NO and anti-AChE
effects of PG, especially from the docking simulation
PG towards AChE that was described for the first time
in this study. The above results suggest that SHP is a
good material for the cost-effective bioproduction of
PG, which is a potential candidate for anti-NO and
anti-Alzheimer drugs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fronte:2021:FRH,
author = "Baldassare Fronte and Rosario Licitra and Carlo
Bibbiani and Lucia Casini and Mahanama De Zoysa and
Vincenzo Miragliotta and Simona Sagona and Francesca
Coppola and Letizia Brogi and Francesca Abramo",
title = "Fishmeal Replacement with \bioname{Hermetia illucens}
Meal in Aquafeeds: Effects on Zebrafish Growth
Performances, Intestinal Morphometry, and Enzymology",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "28",
day = "05",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030028",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/28",
abstract = "Fishmeal (FM) is still the most important protein
source in aquafeeds. However, due to the reduction of
wild fish stocks used for FM production, its
manufacturing it is now unsustainable. Insect meal
represents a valid alternative to FM, due to the low
carbon footprint of its production and its high
nutritional value. The aim of this study was to
investigate the potentials of replacing FM with black
soldier fly (\bioname{Hermetia illucens}) meal (HIM) in
aquafeeds, using zebrafish as the animal model. Four
diets were formulated with increasing HIM/FM
replacement rate (the control diet contained 20\% FM).
Mortality rates, growth performances, and feed
consumptions were measured, and fish intestine samples
were collected for histological and enzymatic analysis.
After 49 days, all groups almost tripled their initial
body weight (cumulative body weight gain ranged between
143.9 and 155.2 mg), and no statistically significant
differences among treatments were observed in relation
to growth performances and histological traits.
Contrarily, trypsin, alkaline phosphatases, and alpha
amylase--glucoamylase activities were significantly
reduced when the FM replacement rate was increased. In
conclusion, HIM may represent an alternative to FM,
since no adverse effects were observed when it was
included up to 20\% in the zebrafish diet, even when
replacing 100\% of the FM.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kidd:2021:ESD,
author = "Jess A. Kidd and Monica Boudreau and Robert C. Bailey
and Michael R. van den Heuvel and Mark R. Servos and
Simon C. Courtenay",
title = "Evaluating the Sampling Design of a Long-Term
Community-Based Estuary Monitoring Program",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "27",
day = "02",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030027",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/27",
abstract = "Community-based monitoring programs (CBMPs) are a
cost-effective option to collect the long-term data
required to effectively monitor estuaries. Data quality
concerns have caused some CBMP datasets, which could
fill knowledge gaps for aquatic ecosystems, to go
unused. The Community Aquatic Monitoring Program (CAMP)
is a CBMP that has collected littoral nekton assemblage
data from estuaries in the southern Gulf of St.
Lawrence since 2003. Concerns with the CAMP sampling
design (station placement and numbers) have prevented
decision-makers from using the data to inform estuary
health assessments. This study tested if CAMP's
sampling design that accommodates volunteer
participation provides similar information as a
scientific sampling approach. Six CAMP stations and six
stations selected using a stratified random design were
sampled at ten estuaries. A permutational-MANOVA
revealed nekton assemblages were generally not
significantly different between the two sampling
designs. The current six CAMP stations are sufficient
to detect the larger differences in species abundances
that may indicate differences in estuary condition. The
predicted increase in precision (2\%) with twelve
stations is not substantive enough to warrant an
increased sampling effort. CAMP's scientific utility is
not limited by station selection bias or numbers.
Furthermore, well-designed CBMPs can produce comparable
data to scientific studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rusco:2021:CMB,
author = "Giusy Rusco and Michele {Di Iorio} and Roberta
Iampietro and Alessandra Roncarati and Stefano Esposito
and Nicolaia Iaffaldano",
title = "Cryobank of \geoname{Mediterranean} Brown Trout Semen:
Evaluation of the Use of Frozen Semen up to Six Hours
Post-Collection",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "26",
day = "02",
month = aug,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030026",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/26",
abstract = "The aim of this study was to evaluate the effects of
different cold-storage time intervals between
collection and semen-freezing on both fresh and
cryopreserved semen motility parameters and the
post-thaw fertilizing ability of Mediterranean brown
trout semen. The ejaculates were split into six
aliquots and stored on ice from 1 to 6 h, until
freezing. Fresh and post-thaw sperm motility was
evaluated by a Computer-Assisted Sperm Analysis system,
whilst the fertilizing ability was assessed by in vivo
trials. In fresh semen, at 3 h of storage, a
significant decrease of total motility, linear movement
(STR, LIN) and beat cross frequency (BCF) was recorded,
whilst the amplitude of lateral displacement of the
spermatozoon head (ALH) underwent a significant
increase. In frozen semen, no significant difference
was observed for all the motility parameters evaluated,
except for the total motility between 1 and 6 h of
storage and the duration of sperm movement between 1
and 5 h. Cold-storage time did not significantly affect
the percentage of live embryos following the use of
frozen semen. In conclusion, our results showed that,
if necessary, the Mediterranean brown trout semen can
be frozen even until 6 h post-collection without losing
its fertilizing ability.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pires:2021:MTE,
author = "Luana da Costa Pires and Patricia Rodrigues and Quelen
Iane Garlet and Luisa Barichello Barbosa and Bibiana
Petri da Silveira and Guerino Bandeira Junior and
Lenise de Lima Silva and Amanda Gindri and Rodrigo
Coldebella and Cristiane Pedrazzi and Agueda Palmira
Castagna de Vargas and Bernardo Baldisserotto and Berta
Maria Heinzmann",
title = "\bioname{Maclura tinctoria} Extracts: In Vitro
Antibacterial Activity against \bioname{Aeromonas
hydrophila} and Sedative Effect in \bioname{Rhamdia
quelen}",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "25",
day = "27",
month = jul,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030025",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/25",
abstract = "Maclura tinctoria is a tree species native from Brazil
and rich in phenolic compounds. Since plant
antibacterial activity is highly associated with
phenolic compound concentration, we aim to evaluate the
in vitro antimicrobial activity of different extracts
against fish pathogenic bacteria. In addition, some
phenolic compounds have central depressant effects and
can be useful in aquaculture due to possible sedative
and/or anesthetic effects. Four M. tinctoria extracts
were extracted separately with ethanol; leaves (LE),
bark (BE), heartwood (HE), and the sapwood (SE). In
vitro antimicrobial activity was tested against
Aeromonas strains at concentrations of 6400 to 3.125
\mu g/mL. The sedative effect was evaluated for 24 h
with 30 and 100 mg/L concentrations. Chemical
composition was analyzed by HPLC-DAD-MS. The HE extract
had the best MIC (400 $ \mu $ g/mL) and MBC (800 $ \mu
$ g/mL) compared to the LE, BE, and SE extracts. LE
extract induced deep sedation and the BE, SE, and HE
extracts induced light sedation. Additionally, BE, SE,
and HE induced a normal behavior without side effects.
Polyphenolic compounds with antimicrobial activity and
sedative effects were identified mainly in HE. Thus, HE
extract is safe and can be used as a sedative for
silver catfish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Giovos:2021:ILB,
author = "Ioannis Giovos and Fabrizio Serena and Dimitra Katsada
and Athanasios Anastasiadis and Adi Barash and Charis
Charilaou and Jason M. Hall-Spencer and Fabio Crocetta
and Alexander Kaminas and Demetris Kletou and Mary
Maximiadi and Vasileios Minasidis and Dimitrios K.
Moutopoulos and Roxani Naasan Aga-Spyridopoulou and
Ioannis Thasitis and Periklis Kleitou",
title = "Integrating Literature, Biodiversity Databases, and
Citizen-Science to Reconstruct the Checklist of
Chondrichthyans in {Cyprus (Eastern Mediterranean
Sea)}",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "24",
day = "26",
month = jul,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030024",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/24",
abstract = "Chondrichthyans are apex predators influencing the
trophic web through a top-down process thus their
depletion will affect the remaining biota.
Notwithstanding that, research on chondrichthyans is
sparse or data-limited in several biogeographic areas
worldwide, including the Levantine Sea. We revise and
update the knowledge of chondrichthyans in Cyprus based
on a bibliographic review that gains information
retrieved from peer-reviewed and grey literature,
Global Biodiversity Information Facility (135 records
of at least 18 species) and the Ocean Biodiversity
Information System (65 records of at least14 species),
and the citizen science project Mediterranean
Elasmobranchs Citizen Observations (117 records per 23
species). Our updated checklist reports 60 species that
account for about 70\% of the Mediterranean
chondrichthyan biota. The list includes 15 more species
than the previous checklist and our study reports three
new species for Cyprus waters, namely the blackmouth
catshark \bioname{Dalatias licha}, the round fantail
stingray Taeniurops grabatus, and the sawback
angelshark Squatina aculeata. Our research highlights
the need for conservation measures and more studies
regarding the highly threatened blackchin guitarfish
Glaucostegus cemiculus and the devil ray
\bioname{Mobula mobular}, and stresses the importance
for training a new generation of observers to
strengthen the knowledge and conservation of
elasmobranchs in the region.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sorensen:2021:GCC,
author = "Solveig Lysfjord S{\o}rensen and Ateshm Ghirmay and
Yangyang Gong and Dalia Dahle and Ghana Vasanth and
Mette S{\o}rensen and Viswanath Kiron",
title = "Growth, Chemical Composition, Histology and
Antioxidant Genes of {Atlantic Salmon} (\bioname{Salmo
salar}) Fed Whole or Pre-Processed
\bioname{Nannochloropsis oceanica} and
\bioname{Tetraselmis} sp.",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "23",
day = "23",
month = jul,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030023",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/23",
abstract = "New sustainable feed ingredients are a necessity for
the salmon aquaculture industry. In this study, we
examined the effect of pre-extrusion processing of two
microalgae, Nannochloropsis oceanica and Tetraselmis
sp., on the growth, fatty acid content in the flesh and
health of Atlantic salmon. The fish were fed one of the
following five diets for nine weeks: (1) CO: a fish
meal-based control (basal) diet, (2) NU: a
Nannochloropsis diet, (3) NE: a pre-extruded
Nannochloropsis diet, (4) TU: a Tetraselmis diet, and
(5) TE: a pre-extruded Tetraselmis diet. The
algae-incorporated diets contained 30\% of the
respective microalgae. Our results showed that the best
growth performance was achieved by the CO diet,
followed by the NE diets. Feeding of unprocessed
Nannochloropsis and Tetraselmis resulted in a
significant reduction in enterocyte vacuolization
compared to the CO feeding. A significant effect of
processing was noted in the fillet fatty acid content,
the intestine and liver structure and the expression of
selected genes in the liver. The expression of
antioxidant genes in both the liver and intestine, and
the accumulation of different fatty acids in the fillet
and liver of the extruded algae-fed groups, warrants
further investigation. In conclusion, based on the
short-term study, 30\% inclusion of the microalgae
\bioname{Nannochloropsis oceanica} and Tetraselmis sp.
can be considered in Atlantic salmon feeds.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{He:2021:NRD,
author = "Qingsong He and Shuyang Ma and Qinwang Xing and
Wenchao Zhang and Haiqing Yu and Zhenjiang Ye",
title = "Nonstationary Responses of Demersal Fishes to
Environmental Variations in Temperate Waters of the
{Northwestern North Pacific} under a Changing Climate",
journal = j-FISHES,
volume = "6",
number = "3",
pages = "22",
day = "22",
month = jul,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6030022",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/3/22",
abstract = "Although nonstationarity in marine ecosystems has
attracted great attention, the nonstationary responses
of demersal fishes to environmental variations induced
by the changing climate are still not well understood.
Here, we collected 21 time series of demersal fishes
from 1956 to 2015 to investigate the climate-induced
nonstationary responses in temperate waters of the
northwestern North Pacific (NWP). We showed that these
demersal fishes experienced state shifts in
approximately 1986/87, accompanied by abrupt warming
over this region. Moreover, the relationships between
demersal fishes and sea surface temperature (SST) were
found to change between the two identified eras (i.e.,
a weak relationship before 1986 and a strong negative
relationship after 1986), which may be primarily caused
by the alternating dominance of the East Asian winter
monsoon and mega-ENSO on SST in temperate waters of the
NWP. The identified climate-induced nonstationary
responses of demersal fishes to SST variability in this
study may provide implications for understanding
climate-induced biological dynamics, predicting
demersal fish fluctuations, coping with potential
ecological risks, and the sustainable exploitation of
fishery resources in the future climate. Note that the
impact of fishing on the demersal fishes in temperate
waters of the NWP was not assessed in this study due to
the lack of fishing effort data and therefore the
conclusions of our research should be approached with
caution.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Abu-Alya:2021:EPS,
author = "Ibrahim S. Abu-Alya and Yousef M. Alharbi and Said I.
Fathalla and Ibrahim S. Zahran and Saad M. Shousha and
Hassan A. Abdel-Rahman",
title = "Effect of Partial Soybean Replacement by Shrimp
By-Products on the Productive and Economic Performances
in \geoname{African} Catfish (\bioname{Clarias lazera})
Diets",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "84",
day = "20",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040084",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/84",
abstract = "The present study was carried out to evaluate the
effects of partial replacement of soybean with shrimp
by-products (SBp) in African catfish (\bioname{Clarias
lazera}) diets on productive (growth, digestibility,
body composition, dressing yield, blood parameters,
immunity) and economic performances. Therefore, 750
fingerlings ($ \approx $20.0 g) were distributed into
five groups of 150 fingerlings/group with three
replicates (n = 50) per group. Group 1 was fed a basal
diet, while G2 and G3 were fed diets containing 25\%
SBp (autoclaved and acid-treated, respectively) as
soybean replacer for 12 weeks, while, in G4 and G5, SBp
level was 50\%. All growth and nutrient utilization
parameters (body weight, Wg, ADG, PER, FCR, and
digestibility), plasma proteins, and immunity
significantly increased in G4 and G5. Anemia screening
markers recorded insignificant differences between the
tested groups and control one, whereas lipid markers of
plasma and fish body decreased by autoclaved SBp and
increased by acid-treated SBp. The African catfish body
dry matter and crude protein percentages were enriched
by the addition of SBp. Replacing the soybean meal
decreases diet costs and diet costs/1 kg of weight
gain. In conclusion, replacing soybean with SBp
(especially 50\% acid-treated) positively influenced
productive and economic performances with friendly
effects to avoid the environmental pollution by these
wastes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fu:2021:IEC,
author = "Lulu Fu and Qiudie Chi and Yongbo Bao and Hanhan Yao
and Zhihua Lin and Yinghui Dong",
title = "Identification and Expression Characterization of the
\gene{Smad3} Gene and {SNPs} Associated with Growth
Traits in the Hard Clam (\bioname{Meretrix meretrix})",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "83",
day = "16",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040083",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/83",
abstract = "It has been demonstrated that the sekelsky mothers
against decapentaplegic homolog 3 (Smad3) plays an
important role in the growth and development of
vertebrates. However, little is known about the
association between the Smad3 gene and the growth
traits of mollusks. In this study, Smad3 from the hard
clam \bioname{Meretrix meretrix} (Mm-Smad3) was cloned,
characterized, and screened for growth-related single
nucleotide polymorphisms (SNPs) in its exons. The
full-length cDNA of Mm-Smad3 was 1938 bp, encoding a
protein with 428 amino acid residues. The protein
sequence included an MH1 (27--135 aa) and MH2 domain
(233--404 aa). Promoter analysis showed that the
promoter sequence of Mm-Smad3 was 2548 bp, and the
binding sites of Pit-1a, Antp, Hb, and other
transcription factors are related to the growth and
development of hard clams. The phylogenetic tree was
divided into two major clusters, including mollusks and
vertebrate. The expression level of Mm-Smad3 was
predominantly detected in the mantle and foot, while
extremely less expression was observed in the digestive
gland. The low expression level of Mm-Smad3 was
detected at the stages of unfertilized mature eggs,
fertilized eggs, four-cell embryos, blastula,
gastrulae, trochophore, and D-shaped larvae, whereas an
opposite trend was observed regarding the highest
expression at the umbo larvae stage (p {$<$} 0.05). In
the mantle repair experiment, the time-course
expression profiles showed that compared to the
expression level at 0 h, Mm-Smad3 significantly
decreased at 6 h (p {$<$} 0.05) but increased at 12 and
48 h. Further, the association analysis identified 11
SNPs in the exons of Mm-Smad3, of which three loci
(c.597 C $>$ T, c.660 C $>$ T, c.792 A $>$ T) were
significantly related to the growth traits of clam (p
{$<$} 0.05). Overall, our findings indicated that
Mm-Smad3 is a growth-related gene and the detected SNP
sites provide growth-related markers for molecular
marker-assisted breeding of this species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jeong:2021:CGS,
author = "Min-A Jeong and Ye-Jin Jeong and Kwang-Il Kim",
title = "Complete Genome Sequences and Pathogenicity Analysis
of Two Red Sea Bream Iridoviruses Isolated from
Cultured Fish in \geoname{Korea}",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "82",
day = "15",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040082",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/82",
abstract = "In Korea, red sea bream iridovirus (RSIV), especially
subtype II, has been the main causative agent of red
sea bream iridoviral disease since the 1990s. Herein,
we report two Korean RSIV isolates with different
subtypes based on the major capsid protein and
adenosine triphosphatase genes: 17SbTy (RSIV mixed
subtype I/II) from Japanese seabass
(\bioname{Lateolabrax japonicus}) and 17RbGs (RSIV
subtype II) from rock bream (\bioname{Oplegnathus
fasciatus}). The complete genome sequences of 17SbTy
and 17RbGs were 112,360 and 112,235 bp long,
respectively (115 and 114 open reading frames [ORFs],
respectively). Based on nucleotide sequence homology
with sequences of representative RSIVs, 69 of 115 ORFs
of 17SbTy were most closely related to subtype II
(98.48--100\% identity), and 46 were closely related to
subtype I (98.77--100\% identity). In comparison with
RSIVs, 17SbTy and 17RbGs carried two insertion/deletion
mutations (ORFs 014R and 102R on the basis of 17SbTy)
in regions encoding functional proteins (a DNA-binding
protein and a myristoylated membrane protein). Notably,
survival rates differed significantly between
17SbTy-infected and 17RbGs-infected rock breams,
indicating that the genomic characteristics and/or
adaptations to their respective original hosts might
influence pathogenicity. Thus, this study provides
complete genome sequences and insights into the
pathogenicity of two newly identified RSIV isolates
classified as a mixed subtype I/II and subtype II.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sanz-Ronda:2021:FUP,
author = "Francisco Javier Sanz-Ronda and Francisco Javier
Bravo-C{\'o}rdoba and Ana Garc{\'\i}a-Vega and Jorge
Valbuena-Castro and Andr{\'e}s Mart{\'\i}nez-de-Azagra
and Juan Francisco Fuentes-P{\'e}rez",
title = "Fish Upstream Passage through Gauging Stations:
Experiences with \geoname{Iberian} Barbel in Flat-{V}
Weirs",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "81",
day = "14",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040081",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/81",
abstract = "The monitoring of river discharge is vital for the
correct management of water resources. Flat-V gauging
weirs are facilities used worldwide for measuring
discharge. These structures consist of a small weir
with a triangular cross-section and a flat ``V''-shaped
notch. Their extensive use is a consequence of their
utility in the measurement of both low and high flow
conditions. However, depending on their size, local
morphology and river discharge can act as full or
partial hydraulic barriers to fish migration. To
address this concern, the present work studies fish
passage performance over flat-V weirs considering their
hydraulic performance. For this, radio-tracking and
video-monitoring observations were combined with
computational fluid dynamics (CFD) models in two flat-V
weirs, using Iberian barbel (Luciobarbus bocagei) as
the target species. Results showed that fish passage is
conditioned by both hydraulic and behavioral processes,
providing evidence for scenarios in which flat-V weirs
may act as full or partial barriers to upstream
movements. For the studied flat-V weirs, a discharge
range of 0.27--8 m 3 /s, with a water drop difference
between upstream and downstream water levels lower than
0.7 m and a depth downstream of the weir of higher than
0.3 m can be considered an effective passage situation
for barbels. These findings are of interest for
quantifying flat-V weir impacts, for engineering
applications and for establishing managing or
retrofitting actions when required.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2021:RBE,
author = "Jinfei Hu and Ping Wang and Hailong Zhang",
title = "The Relationship between Environmental Factors and
Catch Abundance of Hairtail in the \geoname{East China
Sea} Using Empirical Dynamic Modeling",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "80",
day = "13",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040080",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/80",
abstract = "The East China Sea population of hairtail
(\bioname{Trichiurus lepturus}, also known as T.
japonicus) is a commercially important element of
Chinese fisheries. Hairtail has long been widely
exploited. Due to overfishing, however, its production
declined over the years. One of solutions to this
dilemma is to institute reasonable fishery policies.
Generally, skillful short-term and long-term prediction
of fish catch is a central tool for guiding the
development of fishery policy. Accurate predictions
require a comprehensive understanding of the
relationship between fluctuations in fish catch and
variability in both fishing effort and marine
environmental conditions. To investigate the combined
impact of fishing effort and marine environments on
hairtail catch and to develop models to predict
hairtail catch, we applied empirical dynamic modeling
(EDM) to data on East China Sea fisheries, including
hairtail catch, fishing effort, and marine
environmental factors. EDM is an equation-free approach
that enables the investigation of various complex
systems. We constructed all possible multivariate EDM
models to investigate the potential mechanisms
affecting hairtail catch. Our analysis demonstrates
that all key environmental factors (salinity, summer
monsoon, sea surface temperature, precipitation, and
power dissipation index of tropical cyclones) have an
impact on nutrient supply, which we suggest is the
central factor influencing hairtail catch. Finally, our
comparison of EDM models with parametric models
demonstrates that EDM models overwhelmingly outperform
parametric models in analysis of these complex
interactions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shan:2021:CTA,
author = "Binbin Shan and Yan Liu and Changping Yang and Yuan Li
and Liangming Wang and Dianrong Sun",
title = "Comparative Transcriptome Analysis of Female and Male
Fine-Patterned Puffer: Identification of Candidate
Genes Associated with Growth and Sex Differentiation",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "79",
day = "12",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040079",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/79",
abstract = "Sexual growth dimorphism is a common phenomenon in
teleost fish. However, the mechanism of this complex
phenomenon remains unclear. The fine-patterned puffer
(Takifugu poecilonotus; Temminck and Schlegel, 1850)
exhibits female-biased sexual size dimorphism similar
to other pufferfish. In this study, the transcriptomes
of female and male T. poecilonotus were sequenced,
285.95 million raw read pairs were generated from
sequence libraries. After identification and assembly,
a total of 149,814 nonredundant unigenes were obtained
with an N50 length of 3538 bp. Of these candidates,
122,719 unigenes (81.91\% of the total) were
successfully annotated with multiple public databases.
The comparison analysis revealed 10,385 unigenes (2034
in females and 8351 in males) were differentially
expressed between different sexes of T. poecilonotus.
Then, we identified many candidate growth- and
sex-related genes, including Dmrt1, Sox3, Spatas, Prl /
Prlr, fabps, Ghr, and Igf1r. In addition to these
well-known genes, Fabp 4 was identified for the first
time in fish. Furthermore, 68,281 simple sequence
repeats (SSRs) loci were screened and identified in the
transcriptome sequence of T. poecilonotus. The results
of our study could provide valuable information on
growth- and sex-associated genes and facilitate further
exploration of the molecular mechanism of sexual growth
dimorphism.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nousias:2021:GVP,
author = "Orestis Nousias and Konstantinos Tzokas and Leonidas
Papaharisis and Katerina Ekonomaki and Dimitrios
Chatziplis and Costas Batargias and Costas S.
Tsigenopoulos",
title = "Genetic Variability, Population Structure, and
Relatedness Analysis of Meagre Stocks as an Informative
Basis for New Breeding Schemes",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "78",
day = "10",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040078",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/78",
abstract = "This study evaluates the genetic diversity of
different meagre broodstocks sampled in Greece. A
multiplex of twelve microsatellite markers was used to
genotype 946 fish from eleven stocks and batches used
for broodstock selection, and the genetic data was used
to calculate genetic population parameters as well as
to investigate the genetic differentiation between
stocks. The results from a relatedness analysis were
used as the guiding lines for a fine-tuned and overall
evaluation of the genetic distance between stocks, and
the choice of candidate breeders from some of them. The
approach implemented in this study uses
well-established population genetics methods to
evaluate the selection of breeder candidates in
aquaculture commercial conditions utilizing a
descriptive genetic data set based on microsatellite
analyses, and to outline an efficient methodology for
establishing the basis of new breeding schemes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mao:2021:MCN,
author = "Zhuangwen Mao and Shengwei Luo and Dafang Zhao and
Xiang Zhou and Zilong Zhang and Yangbo Xiao and
Shenping Cao and Yonghua Zhou and Shaojun Liu and
Jianzhou Tang and Zhen Liu",
title = "Molecular Characterization and Nutrition Regulation of
the Neutral Amino Acid Transporter {ASCT2} in Triploid
Crucian Carp",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "77",
day = "09",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040077",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/77",
abstract = "AlaSerCys Transporter 2 (ASCT2), encoded by the SLC1A5
gene, plays an important role in the absorption of
glutamine. In this study, the full-length cDNA sequence
of ASCT2 was cloned from triploid crucian carp. It
encodes 539 amino acid residues and a stop codon.
Phylogenetic analysis revealed that the sequences of
the ASCT2 ORF region in cyprinid fishes shared high
sequence homology. Comparing the abundance of ASCT2 in
different tissues, we found its expression level in
muscle was significantly higher than that in intestine
(p {$<$} 0.05). In addition, the expression levels of
ASCT2 also appeared different in diurnal variation.
Then we found the addition of 2.5\% glutamate in a
feeding diet significantly increased the expression
levels of ASCT2 in intestine and muscle (p {$<$} 0.05).
However, in glutamine experiments, the muscle showed
the highest expression level of ASCT2 when fish were
fed the diet containing 3.0\% glutamine (p {$<$} 0.05).
In vitro, ASCT2 was sensitive to glutamine and its
expression level appeared down-regulated when the
addition of glutamine was added to 0.1 mg/mL. Finally,
we found that the diet with 29\% protein level
significantly increased the expression level of ASCT2
in intestine (p {$<$} 0.05). Nevertheless, different
protein sources (fish meal and soybean meal) had no
significant effect on the expression levels of ASCT2 in
intestine and muscle (p $>$ 0.05). These results
provided data for the study of ASCT2 in triploid
crucian carp regulated by feeding nutrition, which had
a potential application in improving feed formulation
in aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nathanailides:2021:PPS,
author = "Cosmas Nathanailides and Markos Kolygas and
Konstantina Choremi and Theodoros Mavraganis and
Evangelia Gouva and Kosmas Vidalis and Fotini
Athanassopoulou",
title = "Probiotics Have the Potential to Significantly
Mitigate the Environmental Impact of Freshwater Fish
Farms",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "76",
day = "08",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040076",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/76",
abstract = "Probiotics for freshwater fish farming can be
administered as single or multiple mixtures. The
expected benefits of probiotics include disease
prophylaxis, improved growth, and feed conversion
parameters, such as the feed conversion rate (FCR) and
specific growth rate (SGR). In the current work, we
review the impact of probiotics on freshwater finfish
aquaculture. Data were gathered from articles published
during the last decade that examined the effects of
probiotics on fish growth, FCR, and water quality in
freshwater fishponds/tanks. While the expected benefits
of probiotics are significant, the reviewed data
indicate a range in the level of effects, with an
average reduction in ammonia of 50.7\%, SGR increase of
17.1\%, and FCR decrease of 10.7\%. Despite the
variability in the reported benefits, probiotics appear
to offer a practical solution for sustainable
freshwater aquaculture. Disease prophylaxis with
probiotics can reduce the need for antibiotics and
maintain gut health and feed conversion. Considering
that fish feed and waste are two significant parameters
of the aquaculture ecological footprint, it can be
argued that probiotics can contribute to reducing the
environmental impact of aquaculture. In this direction,
it would be beneficial if more researchers incorporated
water quality parameters in future aquaculture research
and protocols to minimize aquaculture's environmental
impact.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ciepiela:2021:BIB,
author = "Lindsy R. Ciepiela and Ryan M. Fitzpatrick and Samuel
T. Lewis and Yoichiro Kanno",
title = "Behavioral Interactions between a Native and an
Invasive Fish Species in a Thermally Heterogeneous
Experimental Chamber",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "75",
day = "07",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040075",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/75",
abstract = "Mechanisms of the displacement of native fish by
nonnative fish can include agonistic behaviors that
push native fish species out of their preferred
habitat, including their thermal optima. To examine
these interactions, we built an experimental thermal
preference chamber to evaluate: (1) the thermal
preference of native, glacial relict northern redbelly
dace \bioname{Chrosomus eos}; (2) if the thermal
preference and movement changed in the presence of the
invasive western mosquitofish \bioname{Gambusia
affinis}; and (3) the direction of agonistic
interactions. We hypothesized that G. affinis would
express agonistic behavior toward C. eos, because G.
affinis is widely recognized as an aggressive invader.
Given the temperature range of the experimental
chamber, i.e., 20--30 {$^\circ $}C, C. eos selected an
average of 24.3 {$^\circ $}C as its thermal preference.
After G. affinis ' introduction, the thermal preference
of C. eos increased by 1.7 {$^\circ $}C and the
movement, given by distance (cm) travelled, increased
by 21\%. Contrary to our prediction, more agonistic
interactions were observed in C. eos toward G. affinis.
These results indicate that agonistic behavior of G.
affinis toward native fish species may be species- and
condition-specific, and may not always be the primary
mechanism of native species' displacement. Biological
invasions are a global issue and altered thermal
regimes are expected to continue. This study provided
the novel approach using of a thermally heterogeneous
thermal chamber to examine thermal preferences and
aggressive interactions between a native and an
invasive species. Future research should examine other
life history traits that may be conveying the
competitive advantage to G. affinis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shal:2021:DFA,
author = "Ahmed Mohamed {El Shal} and Faisal Mohamed {El Sheikh}
and Atef Mohamed Elsbaay",
title = "Design and Fabrication of an Automatic Fish Feeder
Prototype Suits Tilapia Tanks",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "74",
day = "06",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040074",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/74",
abstract = "The conventional methods of supplying feed to tilapia
tanks are ineffective. It is better to find new a
automatic feeder saving pellets from crushing and
cohesion without hitting pellets during feeding at a
predetermined interval of time and an accurate amount
of food with a larger surface area covered by pellets.
Developing-country fish farmers use manual feeding to
be more cost-effective than with costly mechanized
feeding, so this research aimed to design and construct
an automatic fish feeder prototype to feed tilapia in a
recirculation aquaculture system's tank. The
performance of the prototype was studied after it was
designed and installed. The dispensed feed operated by
a DC motor located underneath the pellet hopper and the
feed material was discharged into the tank through a
gate in the bottom of the feeder. Three pellet sizes,
three rotation speeds, and three feeder heights from
the water's surface were used to test the automatic
feeder's efficiency. The results showed that the
optimal speed for the automatic feeder was 14 rpm with
a height of 70 cm, resulting in a distribution width of
26.6 cm and a high automatic feeder efficiency of
99.9\%. Furthermore, the feeder used very little
electricity and saved time, cost, labor, energy, and
pellets.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Thomson:2021:HGS,
author = "Damian P. Thomson and Anna K. Cresswell and
Christopher Doropoulos and Michael D. E. Haywood and
Melanie Orr and Andrew S. Hoey",
title = "Hidden Giants: the Story of \bioname{Bolbometopon
muricatum} at \geoname{Ningaloo Reef}",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "73",
day = "06",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040073",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/73",
abstract = "\bioname{Bolbometopon muricatum} (bumphead parrotfish,
Valenciennes, 1839) is a conspicuous, iconic and
ecologically important coral reef fish species. B.
muricatum plays an important role in the bioerosion of
the reef framework and as a result has been described
as both an ecosystem engineer and keystone species.
Despite the complete absence of B. muricatum from 32
years of scientific surveys across the Ningaloo Reef
World Heritage Area, we recorded a total of 155
individuals of B. muricatum across 63.2 ha of reef
crest surveys, equating to mean density of 2.38 ind/ha.
Our observations represent the first record of this
iconic species in scientific surveys at Ningaloo and in
combination with qualitative observations of B.
muricatum by expert witnesses, indicate B. muricatum is
likely to have been present in ecologically relevant
densities since 2006. The densities of B. muricatum
observed at northern Ningaloo in 2021 suggest this
species is removing an estimated 13.42 tonnes/ha or
1.34 kg/m 2 of calcium carbonate per year, which is
broadly comparable with estimates of total parrotfish
bioerosion across many reefs in the central Indian and
Pacific Oceans. Although not currently afforded
elevated conservation status within management plans,
B. muricatum possess many life-history characteristics
that make them vulnerable to overfishing and may
justify consideration for increased protection within
the world heritage listed Ningaloo Reef Marine Park.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Castaneda-Chavez:2021:CMN,
author = "Mar{\'\i}a del Refugio Casta{\~n}eda-Ch{\'a}vez and
Fabiola Lango-Reynoso and Gabycarmen
Navarrete-Rodr{\'\i}guez and Armando Toyokazu
Wakida-Kusunoki",
title = "Concentration of Metals in Native and Invasive Species
of Fish in the Fluvial-Lagoon-Deltaic System of the
{Palizada River, Campeche}",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "72",
day = "03",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040072",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/72",
abstract = "Aquatic organisms, such as fish, are important
indicators of the bioavailability of metals in coastal
environments, demonstrating the capacity of different
species to bioaccumulate these metallic elements. The
fluvial-lagoon system of the Palizada River is an
important ecosystem for its terrestrial and aquatic
biological diversity where fishing is an important
productive activity in this system. The objective of
this research was to evaluate the concentration of Pb
and Cd in the muscle tissue of native and invasive fish
species in this area. For this, the digestion of fish
muscle samples was carried out with a CEM MARS 5
Digestion Microwave System, while the quantification of
metals was performed with an atomic absorption
equipment. All analysis muscle samples from native and
invasive fish showed Pb and Cd in their content. By
sampling site there was a statistically significant
difference (p {$<$} 0.05), with a maximum Pb
concentration of 7.760 $ \mu $ g g {-1} at the LLAR
site (Laguna Larga). In terms of species, the maximum
Cd concentration was obtained in the \bioname{Cyprinus
carpio} with 6.630 {\pm} 0.127 and in Pterygoplichthus
pardalis with 6.547 {\pm} 0.873 $ \mu $ g g {-1} (dry
weight). The presence of metals such as Pb and Cd in
muscle tissue of native and invasive fish species
represents an important bioindicator of environmental
exposure in the study area and a potential risk to
public health, as these species are commonly
consumed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Espinola-Novelo:2021:STV,
author = "Juan F. Esp{\'\i}nola-Novelo and Marcelo E. Oliva",
title = "Spatial and Temporal Variability of Parasite
Communities: Implications for Fish Stock
Identification",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "71",
day = "03",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040071",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/71",
abstract = "The spatial and temporal variability of parasite
communities have received little attention when used as
biological tags for identifying fish stocks. This study
evaluated the potential spatial and temporal
variability of the parasite communities affecting three
marine fish species collected between 1993 and 2017. To
avoid the potential effect of host age in parasite
communities, individuals of similar ages were selected:
1123 \bioname{Engraulis ringens} (12--24 months old),
1904 \bioname{Trachurus murphyi} (24--36 months old),
and 630 \bioname{Merluccius gayi} (36--48 months old).
Most taxa show differences in the prevalence at the
spatial and temporal scales, but the prevalence of some
larval endoparasites remains constant at the temporal
scale. At the spatial scale, an analysis of similarity
(ANOSIM) showed differences in the parasite communities
of three species; a canonical analysis of principal
coordinates (CAP) showed low values of correct
allocations (CA; {\SGMLasymp}50\%) and values of
allocation due to chance (AdC) were lower than the CA.
At the temporal scale, an ANOSIM showed differences
between the three species. A CAP showed low values of
CA ({\SGMLasymp}50--60\%) and the AdC was always lower
than CA. Samples at the spatial scale were well
allocated to their localities or nearby localities,
suggesting a spatial stability. Samples from different
years were not well discriminated, suggesting temporal
variability. Therefore, in studies regarding parasites
as a tool for stock identification, temporal
variability must be taken into account.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mukherjee:2021:EPG,
author = "Subham Mukherjee and Old{\v{r}}ich Barto{\v{s}} and
Kamila Zde{\v{n}}kov{\'a} and Petr Han{\'a}k and Petra
Hork{\'a} and Zuzana Musilova",
title = "Evolution of the Parvalbumin Genes in Teleost Fishes
after the Whole-Genome Duplication",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "70",
day = "01",
month = dec,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040070",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/70",
abstract = "Parvalbumin is considered a major fish allergen. Here,
we report the molecular evolution of the parvalbumin
genes in bony fishes based on 19 whole genomes and 70
transcriptomes. We found unexpectedly high parvalbumin
diversity in teleosts; three main gene types
(pvalb-{\alpha}, pvalb-{\beta}1, and pvalb-{\beta}2,
including oncomodulins) originated at the onset of
vertebrates. Teleosts have further multiplied the
parvalbumin gene repertoire up to nine ancestral
copies---two copies of pvalb-{\alpha}, two copies of
pvalb-{\beta}1, and five copies of pvalb-{\beta}2. This
gene diversity is a result of teleost-specific
whole-genome duplication. Two conserved parvalbumin
genomic clusters carry pvalb-{\beta}1 and {\beta}2
copies, whereas pvalb-{\alpha} genes are located
separately in different linkage groups. Further, we
investigated parvalbumin gene expression in 17 tissues
of the common carp (\bioname{Cyprinus carpio}), a
species with 21 parvalbumin genes in its genome. Two
pvalb-{\alpha} and eight pvalb-{\beta}2 copies are
highly expressed in the muscle, while two alternative
pvalb-{\alpha} copies show expression in the brain and
the testes, and pvalb-{\beta}1 is dominant in the
retina and the kidney. The recent pairs of muscular
pvalb-{\beta}2 genes show differential expression in
this species. We provide robust genomic evidence of the
complex evolution of the parvalbumin genes in fishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sorensen:2021:IBC,
author = "Peter W. Sorensen",
title = "Introduction to the Biology and Control of Invasive
Fishes and a Special Issue on This Topic",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "69",
day = "30",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040069",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/69",
abstract = "Across the globe, dozens of species of invasive fish
are now found in fresh as well as marine waters, where
they alter habitats, compete with native fish for food,
and prey on native fishes, exerting both indirect and
direct effects on ecosystems and economies. While
efforts to understand and control these species are
growing, most are still in their infancy; however, a
few examples stand out. This special issue is comprised
of 11 notable articles on freshwater invasive fish and
is the first to address this topic. This introductory
article serves as an introduction to these articles
which focus on 5 topics on invasive freshwater fish:
(1) the damage they cause (one article); (2) techniques
to ascertain their presence (one article); (3)
techniques to restrict their movement (one article);
(4) strategies to control them (three articles); and
(5) lessons learned from ongoing management efforts
(five articles). This introduction notes that
successful management efforts share a few approaches:
(1) they develop and use a deep understanding of local
species and their abundance as well as distribution;
(2) they focus on reducing reproductive success; (3)
they use multiple complimentary control strategies; and
(4) they use a long-term approach.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martin:2021:CPT,
author = "Miriam Martin and Stephen Smith and Michael Kleinhenz
and Geraldine Magnin and Zhoumeng Lin and David Kuhn
and Shawnee Montgomery and Johann Coetzee",
title = "Comparative Pharmacokinetics and Tissue Concentrations
of Flunixin Meglumine and Meloxicam in Tilapia
({Oreochromis} spp.)",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "68",
day = "25",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040068",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/68",
abstract = "Evidence of pain perception in fish is well
established, but analgesic use in aquaculture is
limited. The objective was to investigate the
comparative pharmacokinetics of flunixin administered
intramuscularly (IM) and meloxicam administered IM or
orally (PO) in tilapia. Two hundred and seventy fish
were assigned to 1 of 3 treatment groups: flunixin
meglumine IM (2.2 mg/kg); meloxicam IM (1 mg/kg); or
meloxicam PO (1 mg/kg). Blood and tissue samples were
collected from 6 fish per treatment at 14 time points
for 10 days. Drug concentrations were determined using
ultra-high-pressure liquid chromatography coupled with
mass spectroscopy. Plasma concentration versus time
data were analyzed with a non-compartmental approach
using a commercially available software. Flunixin
reached a mean maximum concentration (C max) of 4826.7
ng/mL at 0.5 h, had a terminal half-life (T 1 / 2) of
7.34 h, and an area under the concentration--time curve
extrapolated to infinity (AUC INF\_obs) of 25,261.62
h{\cdot}ng/mL. Meloxicam IM had a T 1 / 2 of 9.4 h
after reaching a C max of 11.3 ng/mL at 2 h, with an
AUC INF\_obs of 150.31 h{\cdot}ng/mL. Meloxicam PO had
a T 1 / 2 of 1.9 h after reaching a C max of 72.2 ng/mL
at 2 h, with an AUC INF\_obs of 400.83 h{\cdot}ng/mL.
Tissue concentrations of both drugs were undetectable
by 9 h. Flunixin reached a sufficient plasma
concentration to potentially have an analgesic effect,
while meloxicam, when administered at the given dosage,
likely would not.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fitzpatrick:2021:UID,
author = "Ryan M. Fitzpatrick and Dana L. Winkelman and Brett M.
Johnson",
title = "Using Isotopic Data to Evaluate \bioname{Esox lucius}
({Linnaeus}, 1758) Natal Origins in a Hydrologically
Complex River Basin",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "67",
day = "22",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040067",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/67",
abstract = "Otolith microchemistry has emerged as a powerful
technique with which to identify the natal origins of
fishes, but it relies on differences in underlying
geology that may occur over large spatial scales. An
examination of how small a spatial scale on which this
technique can be implemented, especially in water
bodies that share a large proportion of their flow,
would be useful for guiding aquatic invasive species
control efforts. We examined trace isotopic signatures
in northern pike (\bioname{Esox lucius}) otoliths to
estimate their provenance between two reservoirs in the
Upper Yampa River Basin, Colorado, USA. This is a
challenging study area as these reservoirs are only
11-rkm apart on the same river and thus share a high
proportion of their inflow. We found that three
isotopes (86 Sr, 137 Ba, and 55 Mn) were useful in
discriminating between these reservoirs, but their
signatures varied annually, and the values overlapped.
Strontium isotope ratios (87 Sr/ 86 Sr) were different
between sites and relatively stable across three years,
which made them an ideal marker for determining
northern pike provenance. Our study demonstrates the
usefulness of otolith microchemistry for natal origin
determination within the same river over a relatively
small spatial scale when there are geologic differences
between sites, especially geologic differences
underlying tributaries between sites.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2021:EDT,
author = "Bin Wang and Guoxin Ma and Yong Liu and Yafei Wang and
Xiaoxue Du and Qiang Shi and Hanping Mao",
title = "Effects of Different Temperatures on the
Antibacterial, Immune and Growth Performance of Crucian
Carp Epidermal Mucus",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "66",
day = "22",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040066",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/66",
abstract = "Fish is one of the important sources of energy and
protein, and proper water temperature is key to
successful fish breeding. The authors of this study
evaluated crucian carp growth, mucus antibacterial
properties, and immune indicators at 17, 21, 24, 27,
and 31 \degree C. The results indicated that in the
range of 17--31 \degree C, the resistance of epidermal
mucus to \bioname{Vibrio harveyi} decreased with
temperature rising. At 24 and 27 \degree C, the
activities of lysozyme and catalase significantly
increased; alkaline phosphatase activity, superoxide
dismutase activity, and total protein concentration
first increased and then decreased with rising
temperature; the highest values were observed at 24
\degree C, with increases of 56.55\%, 26.64\%, and
44.52\%, respectively, compared to those under the 17
\degree C treatment. When the treatment reached 27
\degree C, the temperature had an effect on the growth
and antibacterial properties of crucian carp, and the
activities of alkaline phosphatase and superoxide
dismutase were significantly reduced. At temperatures
of 17--24 \degree C, the survival rate of crucian carp
could reach more than 93\%, and at the temperature of
24 \degree C, the specific growth rate reached the
highest value of 43.29\%. Therefore, the most favorable
temperature for the long-term breeding of crucian carp
was found to be 24 \degree C. This study provides a
favorable experimental basis for the establishment of
intelligent aquaculture systems and the setting of
water environment parameters.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lin:2021:FRF,
author = "Bin Lin and Kailin Jiang and Zhiqi Xu and Feiyi Li and
Jiao Li and Chaoli Mou and Xinyao Gong and Xuliang
Duan",
title = "Feasibility Research on Fish Pose Estimation Based on
Rotating Box Object Detection",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "65",
day = "19",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040065",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/65",
abstract = "A video-based method to quantify animal posture
movement is a powerful way to analyze animal behavior.
Both humans and fish can judge the physiological state
through the skeleton framework. However, it is
challenging for farmers to judge the breeding state in
the complex underwater environment. Therefore, images
can be transmitted by the underwater camera and
monitored by a computer vision model. However, it lacks
datasets in artificial intelligence and is unable to
train deep neural networks. The main contributions of
this paper include: (1) the world's first fish posture
database is established. 10 key points of each fish are
manually marked. The fish flock images were taken in
the experimental tank and 1000 single fish images were
separated from the fish flock. (2) A two-stage attitude
estimation model is used to detect fish key points. The
evaluation of the algorithm performance indicates the
precision of detection reaches 90.61\%, F1-score
reaches 90\%, and Fps also reaches 23.26. We made a
preliminary exploration on the pose estimation of fish
and provided a feasible idea for fish pose
estimation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ruiz-Jarabo:2021:SPR,
author = "Ignacio Ruiz-Jarabo and Miriam Fern{\'a}ndez-Castro
and Ismael Jerez-Cepa and Cristina
Barrag{\'a}n-M{\'e}ndez and Montse P{\'e}rez and
Evaristo P{\'e}rez and Juan Gil and Jes{\'u}s Canoura
and Carlos Farias and Juan Miguel Mancera and Ignacio
Sobrino",
title = "Survival and Physiological Recovery after Capture by
Hookline: the Case Study of the Blackspot Seabream
(\bioname{Pagellus bogaraveo})",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "64",
day = "17",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040064",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/64",
abstract = "Evaluating the survival of discarded species is
gaining momentum after the new European Common
Fisheries Policy (Article 15 of the European Regulation
No. 1380/2013). This regulation introduced a discard
ban, with an exemption for those species with
demonstrated high survival rates after their capture
and release. Candidate species should be evaluated for
each fishing gear and geographical area. In this study,
we assessed not only survival, but also physiological
recovery rates of blackspot seabream (\bioname{Pagellus
bogaraveo}) below commercial size captured with a
hookline called `` voracera '' in the Strait of
Gibraltar (SW Atlantic waters of Europe). Experiments
onboard a commercial fishing vessel were paralleled
with studies in controlled ground-based facilities,
where the capture process was mimicked, and
physiological recovery markers were described. Our
results confirmed that hookline capture induced acute
stress responses in the target species, such as changes
in plasma cortisol, lactate, glucose, and osmolality.
However, 90.6\% of the blackspot seabreams below
commercial size captured with this fishing gear managed
to survive, and evidenced physiological recovery
responses 5 h after capture, with complete homeostatic
recovery occurring within the first 24 h. Based on this
study, the European Commission approved an exemption
from the discard (EU Commission Delegated Regulation
6794/2018). Thus, the robust methodology described
herein can be an important tool to mitigate the problem
of discards in Europe.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Safari:2021:ESC,
author = "Omid Safari and Mehrdad Sarkheil and Davar Shahsavani
and Marina Paolucci",
title = "Effects of Single or Combined Administration of
Dietary Synbiotic and Sodium Propionate on Humoral
Immunity and Oxidative Defense, Digestive Enzymes and
Growth Performances of \geoname{African} Cichlid
(\bioname{Labidochromis lividus}) Challenged with
\bioname{Aeromonas hydrophila}",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "63",
day = "15",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040063",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/63",
abstract = "The aim of the present study was to investigate the
potential effects of dietary synbiotic (SYN)
(Pediococcus acidilactici + Galactooligosaccharides; 10
g kg \minus 1), sodium propionate (SP; 5, 10 and 20 g
kg \minus 1) and a combination of SYN + SP on the
growth performance, humoral immunity, antioxidant
responses and disease resistance against
\bioname{Aeromonas hydrophila} of African cichlid
(Labidochromis lividus) fingerlings (0.52 \pm 0.05 g)
in a feeding trial lasting 63 days. A completely
randomized design was run with eight treatments,
including 0 (control) and supplemented diets containing
SYN + SP (e.g., 10 + 5, 10 + 10, 10 + 20, 0 + 5, 0 +
10, 0 + 20 and 10 + 10). The lowest feed conversion
ratio value was observed in fish fed the 5 g kg \minus
1 -SP and 10 g kg \minus 1 -SYN (p {$<$} 0.05). The
highest values of protein efficiency ratio and protein
productive value were recorded in fish fed the 10 g kg
\minus 1 -SYN (p {$<$} 0.05). Fish fed the 10 g kg
\minus 1 -SYN diet had the highest activities of
immunity (lysozyme, immunoglobulin) and antioxidant
responses (glutathione peroxidase and superoxide
dismutase) (p {$<$} 0.05). After 28 days
post-challenge, the highest survival rate (57\%) was
recorded in the diet containing 10 g kg \minus 1 SYN
and 5 g kg \minus 1 SP. The results indicated that the
single administration of SYN or combined with SP,
especially at the level of 5 g kg \minus 1 of diet,
enhanced the survival and growth performances, humoral
immune response, antioxidant and digestive enzymes of
African cichlid.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2021:SCP,
author = "Huiqin Chen and Baoliang Bi and Lingfu Kong and Hua
Rong and Yanhua Su and Qing Hu",
title = "Seasonal Changes in Plasma Hormones, Sex-Related Genes
Transcription in Brain, Liver and Ovary during Gonadal
Development in Female Rainbow Trout
(\bioname{Oncorhynchus mykiss})",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "62",
day = "12",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040062",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/62",
abstract = "The purpose of this study was to investigate the
periodic seasonal changes in endocrine activity and
gonadal development of female rainbow trout
(\bioname{Oncorhynchus mykiss}) in a high-altitude
cold-water environment. The fish were sampled monthly
from January to November and the levels of plasma
hormones (estradiol (E 2), cortisol and thyroid
hormones (TH S)) and vitellogenin (VTG) were measured
by ELISA. Moreover, the transcriptions of sex-related
genes in the ovary, brain, and liver were detected by
qRT-PCR. The results showed a seasonal fluctuation of
plasma hormones and VTG together with the development
of the ovary, which reached a peak from August to
October. Similarly, the transcription of hypothalamic
gonadotropin-releasing hormone-2 (cgnrh-2),
hypothalamic gonadotropin-releasing hormone receptors
(gnrhr) and follicle-stimulating hormone (fsh) in the
brain varied from January to September, but the highest
level was detected in September to November. In
addition, the transcription of sex-related genes
located in the ovary and liver increased significantly
during August to October, accompanied by a continuous
increase in the gonadosomatic index (GSI) and a
decrease in the hepatosomatic index (HSI). Therefore,
plasma hormones and sex-related genes regulate the
development and maturation of O. mykiss oocytes with
the change in seasons and peaked in November. The
results of this study provide a reference for improving
the efficiency of the artificial reproduction of
\bioname{O. mykiss}.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garcia-Marquez:2021:ATA,
author = "Jorge Garc{\'\i}a-M{\'a}rquez and Andre Barany and
{\'A}lvaro Broz Ruiz and Benjam{\'\i}n Costas and
Salvador Arijo and Juan Miguel Mancera",
title = "Antimicrobial and Toxic Activity of Citronella
Essential Oil (\bioname{Cymbopogon nardus}), and Its
Effect on the Growth and Metabolism of Gilthead
Seabream (\bioname{Sparus aurata} {L.})",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "61",
day = "12",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040061",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/61",
abstract = "Aquaculture procedures usually induce stress that
affects the physiological status of fish. For this
reason, the inclusion of additives in fish feeds to
palliate stress might be a good alternative. This study
aimed to assess the antimicrobial activity of
citronella (Cymbopogon nardus) essential oil (CEO)
against bacterial pathogens and to determine its
dietary impact on the growth performance of
\bioname{Sparus aurata}. In vitro tests confirmed that
CEO possesses antimicrobial activity against several
fish-specific pathogens. For the in vivo tests, three
experimental groups were fed for 60 days with different
concentrations of CEO: CTRL (0 mL kg \minus 1 fish
feed); CEO1 (1 mL kg \minus 1 fish feed); and CEO2 (2
mL kg \minus 1 fish feed). At the end of the
experiment, the physiological status was characterized.
Subsequently, the specimens of the CTRL and CEO2 groups
were subjected to a challenge with an injection of Poly
I:C for immune stimulation. Although S. aurata
individuals tolerated CEO inclusion without
compromising growth performance, it significantly
reduced glycogen in the CEO2 group, concomitant to an
increment of total peripheral leucocytes. Moreover,
different hematological profiles' responsive patterns
against an inflammatory stimulus were observed. In
conclusion, our results suggest that the use of CEO as
a fish feed additive can prevent bacterial outbreaks
and improve potential in vivo disease resistance in S.
aurata without negatively affecting growth.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Castillo-Vargasmachuca:2021:SDS,
author = "Sergio G. Castillo-Vargasmachuca and Eugenio Alberto
Arag{\'o}n-Noriega and Guillermo
Rodr{\'\i}guez-Dom{\'\i}nguez and Leonardo
Mart{\'\i}nez-C{\'a}rdenas and Eulalio
Ar{\'a}mbul-Mu{\~n}oz and {\'A}lvaro J. Burgos Arcos",
title = "The Standard Deviation Structure as a New Approach to
Growth Analysis in Weight and Length Data of Farmed
\bioname{Lutjanus guttatus}",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "60",
day = "12",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040060",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/60",
abstract = "In the present study, size-at-age data (length and
weight) of marine cage-reared spotted rose snapper
\bioname{Lutjanus guttatus} were analyzed under four
different variance assumptions (observed, constant,
depensatory, and compensatory variances) to analyze the
robustness of selecting the right standard deviation
structure to parametrize the von Bertalanffy, Logistic,
and Gompertz models. The selection of the best model
and variance criteria was obtained based on the
Bayesian information criterion (BIC). According to the
BIC results, the observed variance in the present study
was the best way to parametrize the three
abovementioned growth models, and the Gompertz model
best represented the length and weight growth curves.
Based on these results, using the observed error
structure to calculate the growth parameters in
multi-model inference analyses is recommended.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Thach:2021:TEO,
author = "Keo Sa Rate Thach and Hong Tu Vo and Ji-Yong Lee",
title = "Technical Efficiency and Output Losses in Shrimp
Farming: a Case in {Mekong Delta, Vietnam}",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "59",
day = "11",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040059",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/59",
abstract = "The conversion from black tiger shrimp farming and
crop farming to white leg shrimp production is
widespread in Vietnam's Mekong Delta provinces.
However, this conversion trend also contains many risks
related to the technical aspects of the production
process. The study mainly aims to estimate the
technical efficiency of white leg shrimp farming. It
also compares the technical efficiency of white leg
shrimp farming between the traditional group that
historically has cultivated white leg shrimp and the
group that recently converted from black tiger shrimp
and crops to white leg shrimp, in order to evaluate the
efficiency and adaptability of shrimp farming. Primary
data were collected by directly interviewing 99 farmers
in traditional areas and 101 farmers in recently
converted areas in the study sites and analyzed by
using the stochastic frontier method. The calculated
technical efficiency was around 72.9 percent,
suggesting that farmers could increase their output
level by about 27.1\%, given constant inputs. There was
no difference in the technical efficiency of the two
groups, indicating that the converted farms have
sufficient adaptability when switching to white leg
shrimp farming. The output loss in farmers' production
is still very high, with an average loss of 1973
kg/hectare.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shen:2021:EFD,
author = "Fengyuan Shen and Zonghang Zhang and Yiqiu Fu and Zhen
Zhang and Xin Sun and Jianyu Dong and Xiayang Ding and
Muyan Chen and Xiumei Zhang",
title = "Effects of Food Deprivation Duration on the Behavior
and Metabolism of Black Rockfish (\bioname{Sebastes
schlegelii})",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "58",
day = "06",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040058",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/58",
abstract = "Fish often undergo food shortages in nature,
especially for juveniles that are relatively vulnerable
in most aspects. Therefore, the effects of food
deprivation on fish behavior and physiology are worth
exploring. Here, we investigated the behavioral and
metabolic adaptations of the juvenile black rockfish
\bioname{Sebastes schlegelii} to different durations of
food deprivation. In this study, three treatments were
set: control group, short-term food deprivation (STFD)
group, and long-term food deprivation (LTFD) group. The
rearing lasted for six weeks. During this period,
videos were recorded three times per week to assess the
locomotor activity and aggression. After this, the
fishes' boldness, neophobia, and aggressiveness were
assessed using different behavioral assessment devices,
while the standard metabolic rate (SMR) was measured by
flow-type respirometry. In general, the values of the
four indicators (swimming time, the number of turns,
movement number, and attack number) for the STFD group
were significantly higher than those for the control
and LTFD group during the same period of rearing. In a
subsequent personality assessment, the STFD group was
observed to be significantly bolder in an assessment
measuring boldness than the control and LTFD group
(indicated by time in the circle, swimming time, number
of times through the circle, and frequency in the
circle). The LTFD group was observed to be more
exploratory in the assessment of neophobia (indicated
by duration in the exploratory area, distance from
novelty item, and frequency of stay in the exploratory
area). Indeed, the LTFD group was observed to be
significantly less aggressive in the assessment of
aggressiveness than the control and STFD group
(indicated by attack number, attack range frequency,
first attack, and winner). SMR was noted to be
significantly higher in the STFD group than in the
control and LTFD group. In conclusion, this study
firstly reported the effects of food deprivation
duration on the personalities of black rockfish, as
well as the behavioral and physiological mechanisms.
Thus, we hope to provide insights into the work of
stock enhancement.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2021:IIR,
author = "Yanping Yang and Fengjiao Ma and Juanjuan Dong and
Lianxing Li and Ping Ren and Yuning Zhang and Yatao Wu
and Yinping Wang and Kai Liu and Fang Zhang",
title = "The Innate Immune Response to Infection by
\bioname{Polyascus gregaria} in the Male
\geoname{Chinese} Mitten Crab (\bioname{Eriocheir
sinensis}), Revealed by Proteomic Analysis",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "57",
day = "04",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040057",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/57",
abstract = "The Chinese mitten crab (\bioname{Eriocheir sinensis})
is a representative catadromous invertebrate of the
Yangtze River and a commercial species widely
cultivated in China. Both cultivated and wild crabs
suffer from a variety of parasites and pathogens, which
can result in catastrophic economic losses in
aquaculture revenue. Polyascus gregaria, a parasitic
barnacle with a highly derived morphology, is
specialized in invading these crabs. This study
examines the immunological mechanism in E. sinensis
infected with P. gregaria. Tandem mass tags (TMT), a
specialized method of mass-spectrometry, was used to
analyze the infection by P. gregaria resistance at the
protein level. In the hepatopancreas of infected crabs,
598 proteins differentially expressed relating to
physiological change, of which, 352 were upregulated
and 246 were downregulated. Based on this differential
protein expression, 104 GO terms and 13 KEGG pathways
were significantly enriched. Differentially expressed
proteins, such as ATG, cathepsin, serpin, iron-related
protein, Rab family, integrin, and lectin, are
associated with the lysosome GO term and the
autophagy-animal KEGG pathways, both of which likely
relate to the immune response to the parasitic P.
gregaria infection. These results show the benefit of
taking a detailed, protein-level approach to
understanding the innate immune response of aquatic
invertebrates to macroparasite infection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pradhan:2021:CCC,
author = "Pravata Kumar Pradhan and Dev Kumar Verma and Shrish
Chandra Yadav and Atul Krishna Dev and Thangaraj Raja
Swaminathan and Anutosh Paria and Rajendran Kooloth
Valappil and Neeraj Sood",
title = "Carps, \bioname{Catla catla}, \bioname{Cirrhinus
mrigala} and \bioname{Hypophthalmichthys molitrix} Are
Resistant to Experimental Infection with Tilapia Lake
Virus ({TiLV})",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "56",
day = "01",
month = nov,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040056",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/56",
abstract = "\bioname{Tilapia tilapinevirus}, also known as tilapia
lake virus (TiLV), is an emerging fish virus that
primarily affects tilapines. However, the virus has
also been detected in a few non-tilapines. As tilapia
is generally farmed in polyculture systems along with
carps in South Asian countries, there is a likelihood
that TiLV-infected tilapia can transmit the virus to
the co-cultured species. In view of the above, the
susceptibility of three carp species, namely catla
(\bioname{Catla catla}), mrigal (\bioname{Cirrhinus
mrigala}) and silver carp (\bioname{Hypophthalmichthys
molitrix}) was evaluated vis-{\`a}-vis tilapia,
following experimental infection with TiLV. No clinical
signs and histopathological alterations could be
observed in carps. RT-qPCR revealed that TiLV copy
numbers in liver and brain of all the three carps were
almost negligible and did not show any increase with
time, suggesting that the virus did not replicate in
liver and brain, the target organs of TiLV. Further,
TiLV could not be isolated from pooled liver and brain
tissues of carps using permissive CFF cell line. On the
contrary, in tilapia, typical clinical signs and
histopathological lesions were observed and there was
significant increase in TiLV copy number up to 6 days
post-injection. Furthermore, the virus was successfully
isolated from pooled liver and brain tissue of infected
tilapia. From the above findings, it could be concluded
that C. catla, C. mrigala and H. molitrix are resistant
to TiLV infection and unlikely to be carriers for this
virus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2021:FPW,
author = "Junyi Li and Hao Du and Jinming Wu and Hui Zhang and
Li Shen and Qiwei Wei",
title = "Foundation and Prospects of Wild Population
Reconstruction of Acipenser dabryanus",
journal = j-FISHES,
volume = "6",
number = "4",
pages = "55",
day = "30",
month = oct,
year = "2021",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes6040055",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:27 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/6/4/55",
abstract = "Acipenser dabryanus is an endemic fish inhabiting the
upper reaches of the Yangtze River in China. It is
classified as a first-class nationally protected animal
in China and is listed in the International Union for
Conservation of Nature (IUCN) Red List of Critically
Endangered Species (CR). Recently, there has been a
decrease in natural reproduction of A. dabryanus, and
the wild population is almost extinct. This paper
summarizes the changes observed in the natural
population of A. dabryanus and the factors leading to
its endangerment. Based on the process of artificial
propagation and achievement of relevant protection
goals, this paper presents the concept and technical
framework for reconstruction of the wild population of
A. dabryanus. In addition, by comprehensively reviewing
the research findings and existing problems in the
resource protection and monitoring of A. dabryanus in
recent years, we assessed the possibility of wild
population reconstruction and resource restoration for
A. dabryanus. Reconstruction and restoration measures
for the wild population of A. dabryanus are proposed,
with the aim of providing a scientific basis for the
reconstruction of the natural population and the
improvement and restoration of critical habitat of this
species. Furthermore, it is hoped that this paper will
serve as a reference for the protection and restoration
of other endangered fishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2022:CBF,
author = "Jae Goo Kim and Su Hwan Kim and Jong Young Park and
Su-Hyang Yoo",
title = "Correlation between Feeding Behaviors and Retinal
Photoreceptor Cells of Largemouth Bass,
\bioname{Micropterus salmoides}, in \geoname{Korea}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "25",
day = "19",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010025",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/25",
abstract = "The largemouth bass (\bioname{Micropterus salmoides}),
a food resource in Korea, is a highly voracious
predator, designated as an invasive species. It is both
diurnal and nocturnal, with high adaptability and
reproducibility. Since the predation characteristics
are associated with sensitive dynamic visual acuity, we
examined the ocular structure of the largemouth bass to
investigate the association between photoreceptor cells
and feeding behavior. Furthermore, the comparison of
the prey-tracking ability of M. salmoides with that of
other predatory fish (Coreoperca herzi and
\bioname{Lepomis macrochirus}) with similar ecological
characteristics revealed the structure and arrangement
of photoreceptor cells, typical of a predatory fish in
M. salmoides. The double and single cone cells in the
retina were present in the regular mosaic patterns of
the quadrilateral units, with four double cone cells
surrounding a single cone cell. The photoreceptor
cells, i.e., the rod, single, and double cone cells of
M. salmoides, were smaller (2.3 {\pm} 0.2, 3.82 {\pm}
0.2, and 7.5 {\pm} 0.2 {\mu}m, respectively) than those
of other species (3.1 {\pm} 0.24, 6.6 {\pm} 0.5, and
11.3 {\pm} 0.4 {\mu}m in diameter, respectively, in C.
herzi). A smaller diameter of cone cells allows for a
high-density arrangement of visual cells, possibly
affecting the dynamic visual acuity for prey
tracking.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2022:ICD,
author = "Dunhua Zhang and Miles D. Lange and Craig A. Shoemaker
and Benjamin H. Beck",
title = "Identification and Characterization of Differentially
Expressed {IgM} Transcripts of Channel Catfish
Vaccinated with Antigens of Virulent \bioname{Aeromonas
hydrophila}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "24",
day = "19",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010024",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/24",
abstract = "Channel catfish (\bioname{Ictalurus punctatus}) is the
top species produced in US aquaculture and motile
Aeromonas septicemia, caused by virulent
\bioname{Aeromonas hydrophila} (vAh), is one of the
most severe diseases that afflict catfish farms.
Previously, vaccination of fish with extracellular
proteins (ECP) of vAh was shown to produce a robust
antibody-mediated immune response against vAh
infection. In this study, we analyzed IgM transcripts
that were differentially expressed in the head kidney
and liver of ECP-immunized and mock-immunized (control)
fish with emphasis on a variable domain of heavy chain.
Quantitative PCR analysis indicated that immunized fish
produced significantly more IgM transcripts than
control fish. Full-length IgM heavy chain cDNA was
cloned, which encoded typical IgM peptide, including
signal peptide, variable domain (VH), constant domain
(CH), and carboxyl terminal peptide. Great sequence
diversity was revealed in a VH segment, with the third
complementarity diversity region (CDR3) being most
variable. Using germline VH gene grouping method,
variants (clones) of VH characterized in this study
belonged to nine VH families. The most unique variants
(approximately 49\%) were found in the VH2 family.
Vaccinated fish apparently had more unique variants
than in the control fish. There were 62\% and 79\% of
unique variants in the head kidney and liver of
vaccinated fish, respectively, while 44\% and 27\%
unique variants in the head kidney and liver of control
fish, respectively. Among the unique variants in VH2
family, approximately 87\% of them were found in
vaccinated fish. Two-dimensional gel electrophoresis of
semi-purified IgM protein confirmed that matured IgM
protein was as variable as IgM transcripts identified
in this study, with isoelectric points crossing from 6
to 10. Results of this study provided insight into the
molecular and genetic basis of antibody diversity and
enriched our knowledge of the complex interplay between
antigens and antibodies in Ictalurid catfish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2022:OMD,
author = "Gongpei Wang and Qindong Tang and Zhi Chen and Dingli
Guo and Lei Zhou and Han Lai and Guifeng Li",
title = "Otolith Microchemistry and Demographic History Provide
New Insight into the Migratory Behavior and
Heterogeneous Genetic Divergence of \bioname{Coilia
grayii} in the \geoname{Pearl River}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "23",
day = "17",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010023",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/23",
abstract = "Coilia grayii is the anadromous form of anchovy that
is distributed in the East and South China Seas. It is
a common fish species in the estuarine area of the
Pearl River. Nevertheless, freshwater populations
appear upstream in the Pearl River, but the migratory
pathway has been mostly impeded by dam construction.
Behavioral differences and constrained habitat within
tributaries are suspected of promoting genetic
divergence in these populations. In this study, we
investigated the migratory behavior and genetic
divergence of six populations of C. grayii fragmented
by dams based on the otolith strontium/calcium (Sr/Ca)
ratio, mitochondrial DNA, and microsatellite
genotyping. All populations were in freshwater with low
Sr/Ca ratios, except the estuarine population (Humen
population) hatched in brackish water. Reduced
nucleotide diversity corresponding to distance was
observed. Populations from distant hydrological regions
exhibited a decline in genetic diversity and a
significant difference with the remaining populations
after fitting the isolation by distance model. Pairwise
fixation indices confirmed these results and moderate
and significant differentiation was found between
Hengxian site and downstream sites. Furthermore,
STRUCTURE analyses revealed that all separated
populations exhibited an admixed phylogenetic pattern
except for individuals from the Hengxian locality. The
upstream sites showed significantly increased
resistance to gene flow from the estuarine population
because of isolation by the dam. The results of the
neutrality test and Bayesian skyline plots demonstrated
complex demography---individuals' experienced
historical expansion and partial upper-dam populations
had recently undergone a colonization, forming a new
genetic structure. Accordingly, this study demonstrates
differences in the migration pattern and genetic
differentiation of C. grayii as a consequence of
demographic history and current processes (habitat
fragmentation and colonization).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Seibel:2022:MAE,
author = "Henrike Seibel and Elvis Chikwati and Carsten Schulz
and Alexander Rebl",
title = "A Multidisciplinary Approach Evaluating Soybean
Meal-Induced Enteritis in Rainbow Trout
\bioname{Oncorhynchus mykiss}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "22",
day = "14",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010022",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/22",
abstract = "This study evaluated a diverse range of markers of
feeding stress to obtain a more precise assessment of
the welfare of rainbow trout in relation to inadequate
husbandry conditions. A feeding stress model based on
dietary soybean meal was employed to identify suitable
minimally invasive ``classical'' stress markers,
together with molecular signatures. In a 56-day feeding
experiment, rainbow trout were fed diets containing
different levels of soybean meal. The impact of these
different soybean meal diets on rainbow trout was
assessed by water quality analyses, clinical health
observations, classic growth and performance
parameters, gut histopathology, blood-parameter
measurements and multigene-expression profiling in RNA
from whole blood. Soybean meal-induced enteritis was
manifested phenotypically by an inflammatory reaction
in the posterior section of the intestine and by
diarrhoea in some trout. These inflammatory changes
were associated with decreased supranuclear
vacuolation. The haematocrit values and the levels of
plasma cortisol and circulating lymphocytes in the
blood were increased in trout that had consumed high
amounts of SBM. Notably, the increased haematocrit
depended significantly on the bodyweight of the
individual trout. The transcript levels of certain
genes (e.g., MAP3K1, LYG, NOD1, STAT1 and HSP90AB)
emerged as potentially useful indicators in the blood
of rainbow trout providing valuable information about
inadequate nutrition. The expression-profiling findings
provide a basis for improved, minimally invasive
monitoring of feeding regimens in trout farming and may
stimulate the development of practical detection
devices for innovative aquaculture operations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sikora:2022:DLH,
author = "Logan W. Sikora and Joseph T. Mrnak and Rebecca
Henningsen and Justin A. VanDeHey and Greg G. Sass",
title = "Demographic and Life History Characteristics of Black
Bullheads \bioname{Ameiurus melas} in a North Temperate
{USA} Lake",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "21",
day = "14",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010021",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/21",
abstract = "Black bullheads \bioname{Ameiurus melas} are an
environmentally tolerant omnivorous fish species that
are found throughout much of North America and parts of
Europe. Despite their prevalence, black bullheads are
an infrequently studied species making their biology,
ecology, and life history poorly understood. Although
limited information has been published on black
bullheads, evidence suggests that bullheads can
dominate the fish biomass and have profound influences
on the fish community in some north temperate USA
lakes. The goal of our study was to provide additional
information on black bullhead population demographics,
growth rates, life history characteristics, and
seasonal diet preferences in a northern Wisconsin lake.
Using common fish collection gears (fyke netting,
electrofishing), fish aging protocols, fecundity
assessments, and diet indices, our results suggested
that black bullheads exhibited relatively fast growth
rates, early ages at maturity, moderate fecundity, and
a diverse omnivorous diet. Due to these demographic and
life history characteristics, black bullheads have the
potential to dominate fish community biomass in their
native and introduced range. Results from our study may
inform the management of black bullhead as native and
invasive species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2022:ERT,
author = "Yutong Li and Jiaojiao Kong and Jianyue Ji",
title = "Environmental Regulation, Technological Innovation and
Development of Marine Fisheries---Evidence from Ten
Coastal Regions in {China}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "20",
day = "14",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010020",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/20",
abstract = "This study aims to examine the relationship between
environmental regulation, technological innovation and
the development of China's marine fisheries. We make a
theoretical overview of the relationship between
environmental regulation, technological innovation and
the development of marine fisheries. Using data from 10
coastal regions of China, we calculate the
comprehensive development level of China's marine
fisheries in each region from 2009 to 2018 through the
entropy method. Then, we use a double logit model to
analyze the effects of environmental regulation and
technological innovation on the development level of
China's marine fisheries, and further test the
mediating effect of technological innovation. The
results show that environmental regulations have a
``U-shaped'' effect on the development of China's
marine fisheries. Moreover, technological innovation is
crucial in promoting the development of China's marine
fisheries. Further, we identify the mediating role of
technological innovation, that is, environmental
regulation affects the development of China's marine
fisheries by influencing technological innovation.
These findings offer theoretical support for the
development of China's marine fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ivanauskas:2022:MAC,
author = "Edgaras Ivanauskas and Andrius Skersonas and Vaidotas
Andra{\v{s}}{\=u}nas and Soukaina Elyaagoubi and
Art{\=u}ras Razinkovas-Baziukas",
title = "Mapping and Assessing Commercial Fisheries Services in
the \geoname{Lithuanian} Part of the \geoname{Curonian
Lagoon}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "19",
day = "14",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010019",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/19",
abstract = "The spatial distribution of biomass of main commercial
fish species was mapped to estimate the supply of a
provisioning fishery service in the Curonian lagoon.
Catch per unit effort (CPUE) was used as a proxy to
estimate the efficiency of commercial fishing and,
subsequently, the potential biomass of fishes. The
relationship between distinctive characteristics of the
fishing areas and corresponding commercial catches and
CPUE was analyzed using multivariate analysis. The
total catch values and CPUE used in the analyses were
derived from the official commercial fishery records.
RDE analysis was used to assess the variation of both
catch and CPUE of commercial fish species, while the
percentages of bottom sediment type coverage, average
depth, annual salinity, and water residence time in
each of the fishing squares were used as explanatory
variables. This distance e-based redundancy analysis
allowed for the use of non-Euclidean dissimilarity
indices. Fisheries data spatial distribution map
indicated the lack of coherence between the spatial
patterns of commercial catches and CPUE distribution in
the northern part of the lagoon. Highest CPUE values
were estimated in the central-eastern part of the
lagoon as compared to the western part of the lagoon
where CPUE values were substantially lower. Both total
catch and CPUE appeared not to be related to the type
of bottom habitats statistically while being spatially
correlated in-between. However, the impact of salinity
and water residence time calculated using the 3D
hydraulic circulation model on the distribution of both
CPUE and commercial catches was statistically
significant.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2022:EDE,
author = "Yan Xu and Yiqun Li and Mingyang Xue and Zidong Xiao
and Yuding Fan and Lingbing Zeng and Yong Zhou",
title = "Effects of Dietary \bioname{Enterococcus faecalis}
{YFI-G720} on the Growth, Immunity, Serum Biochemical,
Intestinal Morphology, Intestinal Microbiota, and
Disease Resistance of Crucian Carp (\bioname{Carassius
auratus})",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "18",
day = "12",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010018",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/18",
abstract = "Diseases of crucian carp (\bioname{Carassius auratus})
are closely related to intestinal parameters.
Enterococcus faecalis has strong colonization ability
in the intestinal tract, and produces natural
antibiotics, bacteriocin, and other bacteriostatic
substances, which can effectively inhibit some
pathogenic bacteria and improve the intestinal
microenvironment. This study aimed to assess the
effects of E. faecalis YFI-G720 which was isolated from
the intestinal of crucian carp on the growth, immunity,
intestinal health, and disease resistance of crucian
carp. Fish (48.16 {\pm} 0.55 g) were fed four diets,
commercial diet or diet containing E. faecalis at 10 5
CFU/g (EF1), 10 6 CFU/g (EF2), or 10 7 CFU/g (EF3) for
28 days. The results showed that supplementation of E.
faecalis significantly improved the weight gain ratio
(WGR) and the specific growth rate (SGR) compared with
control group (p {$<$} 0.05). Intestinal mucosal
epithelial cells in EF2 were intact and normal, but
there was obvious vacuolation in CG. Compared with CG,
serum C3 and IgM in EF2 were significantly increased at
the end of the experiment (p {$<$} 0.05), and serum
alkaline phosphatase was significantly higher in all
experimental groups (p {$<$} 0.05). Among studied
immune-related genes, expression was detected by qPCR,
C3, IgM, and IL-1{\beta}were upregulated in all
experimental groups to varying degrees from 14 days,
with highest expression in EF2 at 28 days. Intestinal
microbiota structure analyzed through high-throughput
sequencing, and the results showed that the relative
abundance of Aeromonas and Acinetobacter decreased
while Cetobacterium increased in all experimental
groups, with the greatest changes in EF2. Challenge
tests showed that fish fed E. faecalis were more
resistant to \bioname{Aeromonas veronii} (p {$<$}
0.05). In conclusion, dietary E. faecalis YFI-G720 at
10 6 CFU/g can improve the health status, immune
parameters, intestinal microbiota composition, and
disease resistance of crucian carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2022:DRD,
author = "Xiaomeng Li and Yuanzhen Tan and Zheng Zhang and
Yupeng Huang and Pengfei Mu and Zhengwei Cui and Xinhua
Chen",
title = "Development of Recombinant Dihydrolipoamide
Dehydrogenase Subunit Vaccine against \bioname{Vibrio}
Infection in Large Yellow Croaker",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "17",
day = "11",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010017",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/17",
abstract = "Large yellow croaker (\bioname{Larimichthys crocea}),
an economically important marine fish in China, has
suffered from serious vibriosis, which has resulted in
great economic losses for the large yellow croaker
industry. Vaccination has been considered to be a safe
and effective method to prevent and control vibriosis.
However, due to the complex diversity and serotypes of
the Vibrio genus, the progress of Vibrio vaccine
development is still slow. In this study, we prepared
recombinant Vibrio dihydrolipoamide dehydrogenase
(rDLD) protein and investigated its potential as a
candidate to be a subunit vaccine against Vibrio. The
lysozyme activity and the rDLD-specific antibody level
in sera of large yellow croakers immunized with rDLD
were significantly higher than those in the control
group, and the transcript levels of proinflammatory
cytokines (IL-6, IL-8, IL-1{\beta}), MHC
II{\alpha}/{\beta}, CD40, CD8{\alpha}, IL-4/13A, and
IL-4/13B were significantly up-regulated in the spleen
and head kidney of large yellow croakers immunized with
rDLD, suggesting that rDLD could induce both specific
and nonspecific immune responses in this species. In
addition, rDLD protein increased the survival rate of
large yellow croakers against \bioname{Vibrio
alginolyticus} and \bioname{Vibrio parahaemolyticus},
with the relative percent of survival (RPS) being
74.5\% and 66.9\%, respectively. These results will
facilitate the development of a potential subunit
vaccine against Vibrio in large yellow croaker
aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{laCruz:2022:LDT,
author = "Simrith E. Cordova-de la Cruz and Marta F. Riesco and
Gil Mart{\'\i}nez-Bautista and Daniel Calzada-Ruiz and
Talhia Mart{\'\i}nez-Burguete and Emyr S.
Pe{\~n}a-Mar{\'\i}n and Carlos Alfonso
{\'A}lvarez-Gonzalez and Ignacio Fern{\'a}ndez",
title = "Larval Development in Tropical Gar
(\bioname{Atractosteus tropicus}) Is Dependent on the
Embryonic Thermal Regime: Ecological Implications under
a Climate Change Context",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "16",
day = "11",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010016",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/16",
abstract = "In ectotherm species, environmental temperature plays
a key role in development, growth, and survival. Thus,
determining how temperature affects fish populations is
of utmost importance to accurately predict the risk of
climate change over fisheries and aquaculture, critical
to warrant nutrition and food security in the coming
years. Here, the potential effects of abnormal thermal
regimes (24, 28 and 32 {$^\circ $}C; TR24, TR28, and
TR32, respectively) exclusively applied during
embryogenesis in tropical gar (\bioname{Atractosteus
tropicus}) has been explored to decipher the potential
consequences on hatching and growth from fertilization
to 16 days post-fertilization (dpf), while effects on
skeletal development and body morphology were explored
at fertilization and 16 dpf. Egg incubation at higher
temperatures induced an early hatching and mouth
opening. A higher hatching rate was obtained in eggs
incubated at 28 {$^\circ $}C when compared to those at
24 {$^\circ $}C. No differences were found in fish
survival at 16 dpf, with values ranging from 84.89 to
88.86\%, but increased wet body weight and standard
length were found in larvae from TR24 and TR32 groups.
Thermal regime during embryogenesis also altered the
rate at which the skeletal development occurs. Larvae
from the TR32 group showed an advanced skeletal
development, with a higher development of cartilaginous
structures at hatching but reduced at 16 dpf when
compared with the TR24 and TR28 groups. Furthermore,
this advanced skeletal development seemed to determine
the fish body morphology. Based on biometric measures,
a principal component analysis showed how along
development, larvae from each thermal regime were
clustered together, but with each population remaining
clearly separated from each other. The current study
shows how changes in temperature may induce
craniofacial and morphological alterations in fish
during early stages and contribute to understanding the
possible effects of global warming in early development
of fish and its ecological implications.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Miura:2022:ESD,
author = "Takeshi Miura and Munenori Nishikawa and Yuki Otsu and
Muhammad Fariz Zahir Ali and Atsushi Hashizume and
Chiemi Miura",
title = "The Effects of Silkworm-Derived Polysaccharide
(Silkrose) on Ectoparasitic Infestations in Yellowtail
(\bioname{Seriola quinqueradiata}) and White Trevally
(\bioname{Pseudocaranx dentex})",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "14",
day = "09",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010014",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/14",
abstract = "The effect of silkworm-derived polysaccharide silkrose
on fish ectoparasites was investigated. When juvenile
yellowtail (\bioname{Seriola quinqueradiata}) fed diets
containing silkrose were artificially infected with
\bioname{Benedenia seriolae}, a fish ectoparasite, the
numbers of parasitized B. seriolae were significantly
lower compared to that in fish in the control group
without silkrose treatment. Furthermore, when juvenile
yellowtails were severely infected with B. seriolae, no
mortality was observed in the silkrose-treated group,
compared to more than 60\% in the control group. In
field studies carried out at a fish farm with
yellowtail and white trevally (\bioname{Pseudocaranx
dentex}), oral treatment with silkrose significantly
reduced B. seriolae parasitism in yellowtail and
Caligus longipedis and Neobenedenia girellae parasitism
in white trevally. Silkrose treatment also reduced
blood levels of cortisol, a stress hormone in both
species. The changes in gene expression in the
epidermis of yellowtail by silkrose treatment were also
investigated, showing that the expression of various
genes, including factors involved in immunity, stress
response, and wound healing, was changed by the
treatment. These findings indicate that
silkworm-derived silkrose effectively prevents
infection by external parasites in yellowtail and white
trevally.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Urias-Sotomayor:2022:SRA,
author = "Ricardo Ur{\'\i}as-Sotomayor and Guillermo
Rodr{\'\i}guez-Dom{\'\i}nguez and Jos{\'e} Ad{\'a}n
F{\'e}lix-Ortiz and Gilberto G. Ortega-Liz{\'a}rraga
and Horacio A. Mu{\~n}oz-Rub{\'\i} and Eugenio Alberto
Arag{\'o}n-Noriega",
title = "Stock Reduction Analysis of Bigeye Croaker
Micropogonias megalops in the {Upper Gulf of
California, Mexico}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "15",
day = "08",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010015",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/15",
abstract = "A stock reduction analysis (SRA) of bigeye croaker
Micropogonias megalops was performed based on
commercial catch data. SRA solutions were restricted to
a 2011 bigeye croaker stock biomass estimate of 14,412
t. The viable solution indicated a reduction in stock
of 73.6\% from 1983 to 2020 with an initial biomass of
22,186 t. In addition, a possible effect of
hyperstability of the stock was evaluated by applying
different versions of the Cobb--Douglas catch function.
The most probable function based on a multi-model
selection procedure was the one wherein the catch does
not depend on biomass and is directly proportional to
the applied fishing effort of small boats ($ \approx $7
m) and vessels ($ \approx $24 m). This situation
suggests that in a free access regime, fishing can
deplete the resource until it collapses, without
observing a significant reduction in its catches until
the event is very close.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pan:2022:CFP,
author = "Weicong Pan and Soottawat Benjakul and Chiara
Sanmartin and Alessandra Guidi and Xiaoguo Ying and
Lukai Ma and Xudong Weng and Jin Yu and Shanggui Deng",
title = "Characterization of the Flavor Profile of Bigeye Tuna
Slices Treated by Cold Plasma Using {E-Nose} and
{GC-IMS}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "13",
day = "08",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010013",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/13",
abstract = "To avoid heat, treatment induces numerous
physicochemical changes under severe conditions in the
tuna, cold plasma (CP), as a non-thermal technology,
possess objective potential on tuna processing. The
effect of cold plasma on the volatile flavor compounds
of bigeye tuna (\bioname{Thunnus obesus}) sashimi has
been evaluated using electronic nose (E-nose) and gas
chromatography-ion mobility spectrometry (GC-IMS).
GC--IMS results revealed a total of 33 volatile
compounds in tuna slices. The effect of CP treatment on
tuna flavor was not significant, furthermore CP could
protect volatile freshness compounds such as 1-hexanol.
Principal component analysis (PCA) of the E-nose and
GC--IMS results could effectively differentiate the
effect of storage to tuna sashimi. There was a high
correlation between the E-nose and GC--IMS results,
providing a theoretical basis for establishing the
flavor fingerprint of tuna sashimi.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sarais:2022:EIR,
author = "Fabio Sarais and Ruth Montero and Sven Ostermann and
Alexander Rebl and Bernd K{\"o}llner and Tom
Goldammer",
title = "The Early Immune Response of Lymphoid and Myeloid
Head-Kidney Cells of Rainbow Trout
(\bioname{Oncorhynchus mykiss}) Stimulated with
\bioname{Aeromonas salmonicida}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "12",
day = "07",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010012",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/12",
abstract = "The teleost head kidney is a highly relevant immune
organ, and myeloid cells play a major role in this
organ's innate and adaptive immune responses. Because
of their complexity, the early phases of the innate
immune reaction of fish against bacteria are still
poorly understood. In this study, na{\"\i}ve rainbow
trout were stimulated with inactivated \bioname{A.
salmonicida} and sampled at 12 h, 24 h and 7 d
poststimulation. Cells from the head kidney were
magnetically sorted with a monoclonal antibody mAB21 to
obtain one (MAb21-positive) fraction enriched with
myeloid cells and one (MAb21-negative) fraction
enriched with lymphocytes and thrombocytes. The gene
expression pattern of the resulting cell subpopulations
was analysed using a panel of 43 immune-related genes.
The results show an overall downregulation of the
complement pathway and cytokine production at the
considered time points. Some of the selected genes may
be considered as parameters for diagnosing bacterial
furunculosis of rainbow trout.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xue:2022:MME,
author = "Junren Xue and Tao Jiang and Xiubao Chen and Hongbo
Liu and Jian Yang",
title = "Multi-Mineral Element Profiles in Genuine and
{``Bathing''} Cultured \geoname{Chinese} Mitten Crabs
(\bioname{Eriocheir sinensis}) in {Yangcheng Lake,
China}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "11",
day = "07",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010011",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/11",
abstract = "The authentication of high-quality fishery products
originating from specific geographical regions is
urgently needed worldwide. Chinese mitten crabs
(\bioname{Eriocheir sinensis}), originating from
Yangcheng Lake, are prime counterfeiting targets for
the same reasons. Foreign crabs that are cultured
briefly in the lake, known as ``bathed'' crabs, are
illegally marketed as natives, negatively impacting the
product quality. To establish a method for
distinguishing ``bathed'' and genuine crabs, in this
paper we conduct a comparative investigation by an
Agilent 7500ce ICP-MS on multi-mineral element
profiling of the third pereiopod from genuine and one
month deliberately ``bathing'' cultured crabs. The
profiles of 11 elements were significantly different
between the genuine and foreign crabs before and after
bathing. The discriminant analysis reached 100\%
accuracy to separate the genuine and ``bathed'' crabs
into different groups. Bathing culture was unable to
converge element profiles between the genuine and
foreign crabs. The biogeochemical profiles can be
effective for distinguishing ``bathed'' crabs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Guo:2022:DST,
author = "Jingya Guo and Yongming Li and Yaping Wang and Boyong
Chen and Yingxin Hu and Yasheng Musha and Xiaoyun He
and Tao Tong and Kunlun Huang",
title = "A 90-Day Subchronic Toxicity Study of Consumption of
{GH}-Transgenic Triploid Carp in {Wistar} Rats",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "10",
day = "05",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010010",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/10",
abstract = "Genetic modification (GM) offers an alternative
strategy to conventional animal breeding. The present
study was carried out to investigate the potential
health effects of the consumption of growth
hormone-transgenic triploid carp (GH-ttc) through a
90-day subchronic rodent feeding study. Wistar rats (n
= 10/sex/group) were given formulated diets containing
GH-ttc or non-GM carp at an incorporated rate of 2.5\%,
5\%, or 10\% (w / w) for 90 days. An additional control
group of rats (n = 10/sex/group) was fed a basic rodent
diet. During the 90-day study, clinical observation,
ophthalmic examination, body weight, and food intake
were evaluated. At the end of the study, rats were
killed, and the hematology, serum chemistry, urine
test, necropsy, and histopathology were assessed.
Compared with the non-GM carp and the basic control
groups, no biologically significant differences were
observed on clinical signs of toxicity, body weights,
food intake, hematology, serum chemistry, urinalysis,
organ weight, and histopathology on selected organs for
the GH-ttc group. The results of this 90-day subchronic
feeding study indicated that, at the dose level used in
this study, consumption of GH-ttc showed no subchronic
toxicity to Wistar rats.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Andrasunas:2022:AFM,
author = "Vaidotas Andra{\v{s}}{\=u}nas and Edgaras Ivanauskas
and Arvydas {\v{S}}vag{\v{z}}dys and Art{\=u}ras
Razinkovas-Baziukas",
title = "Assessment of Four Major Fish Species Stocks in the
\geoname{Lithuanian} and {Russian} Parts of {Curonian
Lagoon (SE Baltic Sea)} Using {CMSY} Method",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "9",
day = "03",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010009",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/9",
abstract = "Fisheries in the Curonian Lagoon (1584 km 2 coastal
lagoon in South-East Baltic) can be classified as
artisanal, small-scale, multi-species, and multi-gear.
Such types of fisheries are characterised as data-poor
and require appropriate investigation methods. We used
CMSY (version CMSY\_2019\_9f. R) to assess maximum
sustainable yield (MSY) and related indicators for
freshwater bream, roach, pike-perch, and European
perch. A decline in pike-perch and roach was
identified, while the stocks of freshwater bream and
European perch were sustained. As the CMSY model does
not consider the impact of environmental factors, the
decline in roach stock may be attributed to the
increase in salinity rather than to overfishing. In the
case of freshwater bream and pike-perch, the method
cannot consider the increase of the percentage of
small-sized fishes in catches due to the allowance of
low-selectivity gears in the fishery. Additionally, in
the case of the pike-perch, the model does not take
into account the interannual fluctuations in the
stock-recruitment system. The assessment of the
European perch stock can be considered to be good.
However, the accuracy of CMSY is limited, and it should
be used for fisheries management only in combination
with other methods.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bosi:2022:MCM,
author = "Giampaolo Bosi and Paolo Merella and Barbara J.
Maynard and Bahram Sayyaf Dezfuli",
title = "Microscopic Characterization of the Mucous Cells and
Their Mucin Secretions in the Alimentary Canal of the
Blackmouth Catshark \bioname{Galeus melastomus}
({Chondrichthyes: Elasmobranchii})",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "8",
day = "01",
month = jan,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010008",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/8",
abstract = "Sharks belong to the most primitive group of jawed
vertebrates and have some special structural and
functional features such as a cartilaginous skeleton, a
spiral intestinal valve, and a rectal gland for
osmoregulation. In January 2020, ten specimens of
\bioname{Galeus melastomus}, the Blackmouth catshark,
were collected from the Gulf of Asinara (North
Sardinia, Italy) and the entire alimentary canal was
studied using histochemical reactions to characterize
the mucous cell types. In the alimentary canal of G.
melastomus, mucous cells mainly secrete a mixture of
acidic and neutral mucins. Of the acidic mucins, only
the carboxylated type was present in mucous cells of
the stomach, while the sulfated type predominated in
the esophagus and the intestines. The use of lectins
revealed a distribution of sugar residues in mucins
related to cellular activities of the different regions
of the catshark alimentary canal. The current study is
the first report to characterize the intestinal mucous
cells of G. melastomus and to provide quantitative data
on their different populations in the alimentary
canal.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shertzer:2022:MDS,
author = "Kyle W. Shertzer and Erik H. Williams and Skyler R.
Sagarese",
title = "Modeling Discards in Stock Assessments: Red Grouper
\bioname{Epinephelus morio} in the {U.S. Gulf of
Mexico}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "7",
day = "28",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010007",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/7",
abstract = "To be as accurate as possible, stock assessments
should account for discard mortality in fisheries if it
occurs. Three common approaches to modeling discards in
assessments are to lump dead discards with landings,
treat dead discards as their own fleet, or link them
conversely with landings through use of a retention
function. The first approach (lumping) implicitly
assumes that the selectivity of landings applies also
to discards. In many cases, that assumption is false,
for example, if discards comprise smaller fish than do
landings. The latter two approaches avoid the
assumption by modeling discards explicitly with their
own selectivity pattern. Here, we examine these
approaches to modeling discards. Using a simulation
study, we demonstrate that the two approaches to
modeling discards explicitly can provide identical
results under both static and time-varying conditions.
Then, using a stock assessment case study of red
grouper \bioname{Epinephelus morio} in the U.S. Gulf of
Mexico, we demonstrate that in practice the approaches
to modeling discards can provide different outcomes,
with implications for the resultant management advice.
We conclude by comparing and contrasting the different
approaches, calling for more research to elucidate
which approach is most suitable under various sources
of error typically encountered in discard data.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ghosh:2022:FEA,
author = "Sampat Ghosh and Victor Benno Meyer-Rochow and Chuleui
Jung",
title = "Farming the Edible Aquatic Snail \bioname{Pomacea
canaliculata} as a Mini-Livestock",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "6",
day = "28",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010006",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/6",
abstract = "In the present paper, we describe the farming system
of Pomacea canaliculata, an edible freshwater snail, as
it is practiced by a farmer as mini-livestock in the
vicinity of Andong in Korea. We visited the snail farm
several times in the summer and winter of the year and
conducted interviews with the farm manager using a
semi-structured questionnaire. The farm is housed in
polythene tunnels and uses a tank pen of trench type
made up of propylene and measuring 1 m $ \times $ 2 m $
\times $ 0.5 m (length $ \times $ width $ \times $
height) in size. A regulated inflow of fresh water and
outflow of used water was installed, with water level
not exceeding 5 to 7 cm. As feed of snails, commercial
fish feed is generally provided. The life cycle of the
P. canaliculata might differ in captivity under the
controlled environmental conditions than that of an
individual in the wild environment. The farming system
of snails, particularly P. canaliculata, does not
involve high labor-intensive, high capital investment
and also does not require high through-put cutting edge
technology. In addition to providing nutrient-dense
snail meat, establishing a snailery could therefore
augment the economic condition of farmers in the poorer
regions of the world and encourage sustainability and
biodiversity conservation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lian:2022:GWC,
author = "Jiaying Lian and Liyuan Lv and Hanhan Yao and Zhihua
Lin and Yinghui Dong",
title = "Genome-Wide Characterization and Analysis of
Expression of the Histone Gene Family in Razor Clam,
\bioname{Sinonovacula constricta}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "5",
day = "25",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010005",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/5",
abstract = "The Chinese razor clam (\bioname{Sinonovacula
constricta}), a bivalve species widely distributed in
estuaries and mudflats, is often exposed to extreme
environmental and microbial stresses. Histones are
fundamental components of chromatin and play an
important role in innate immunity, as demonstrated by
its antimicrobial activities in clams. However, little
attention has been paid to histones in bivalves. To
fill this gap, we investigated the genomic
distribution, structural characteristics, conserved
motifs, and phylogenetic relationships of histones in
S. constricta. A total of 114 histone genes were
detected in the S. constricta genome, which were
divided into 25 types in phylogenetic analysis. Among
them, partial histones exhibited a tissue-dependent
expression pattern, indicating that they may be
involved in sustaining the homeostasis of
organs/tissues in adult S. constricta. Furthermore,
mRNA expression of certain histones changed
significantly in S. constricta when infected with
\bioname{Vibrio parahaemolyticus}, suggesting that
histones play a role in the immune defense of S.
constricta. All together, this study on histone genes
in S. constricta not only greatly expands our knowledge
of histone function in the clam, but also histone
evolution in molluscs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez-Antequera:2022:SSH,
author = "Francisca P. Mart{\'\i}nez-Antequera and Isabel
Barranco-{\'A}vila and Juan A. Martos-Sitcha and
Francisco J. Moyano",
title = "Solid-State Hydrolysis ({SSH}) Improves the
Nutritional Value of Plant Ingredients in the Diet of
\bioname{Mugil cephalus}",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "4",
day = "25",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010004",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/4",
abstract = "The possibility of improving the nutritional quality
of plant byproducts (brewers' spent grain and rice
bran) through an enzyme treatment was tested in a
formulated feed for grey mullet (\bioname{Mugil
cephalus}). The enzyme treatment was carried out by
Solid-State Hydrolysis (SSH) using a commercial
preparation including carbohydrases and phytase. A feed
prepared without the treatment and a commercial feed
for carp were used as controls. In a preliminary
short-term trial carried out at laboratory facilities,
fish receiving the enzyme-treated feed showed
significant improvement in both FCR and SGR when
compared to those obtained with the untreated diet,
although both experimental diets presented worse values
than those obtained with the commercial feed. Different
metabolic indicators including higher values of muscle
glycogen and plasmatic triglycerides supported the
positive effect of the enzyme treatment on the
nutritional condition of the fish over those fed on the
diet containing non-treated ingredients. Results of
growth and feed efficiency that were obtained in a
second long-term trial developed for 148 days under
real production conditions evidenced the equivalence
among the experimental and commercial diets and
confirmed that enzyme pretreatment of plant ingredients
by SSH may be a useful procedure to improve the
nutritive value of high fiber plant byproducts when
included in practical diets for this species and others
with similar nutritional features.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Baloch:2022:CSC,
author = "Ali Asghar Baloch and Ehdaa Eltayeb Eltigani
Abdelsalam and Veronika Pia{\v{c}}kov{\'a}",
title = "Cytokines Studied in Carp (\bioname{Cyprinus carpio}
{L.}) in Response to Important Diseases",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "3",
day = "24",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010003",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/3",
abstract = "Cytokines belong to the most widely studied group of
intracellular molecules involved in the function of the
immune system. Their secretion is induced by various
infectious stimuli. Cytokine release by host cells has
been extensively used as a powerful tool for studying
immune reactions in the early stages of viral and
bacterial infections. Recently, research attention has
shifted to the investigation of cytokine responses
using mRNA expression, an essential mechanism related
to pathogenic and nonpathogenic-immune stimulants in
fish. This review represents the current knowledge of
cytokine responses to infectious diseases in the common
carp (\bioname{Cyprinus carpio} {L.}). Given the
paucity of literature on cytokine responses to many
infections in carp, only select viral diseases, such as
koi herpesvirus disease (KHVD), spring viremia of carp
(SVC), and carp edema virus disease (CEVD), are
discussed. \bioname{Aeromonas hydrophila} is one of the
most studied bacterial pathogens associated with
cytokine responses in common carp. Therefore, the
cytokine-based immunoreactivity raised by this specific
bacterial pathogen is also highlighted in this
review.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Miqueleiz:2022:CSG,
author = "Imanol Miqueleiz and Rafael Miranda and Arturo Hugo
Ari{\~n}o and Elena Ojea",
title = "Conservation-Status Gaps for Marine Top-Fished
Commercial Species",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "2",
day = "23",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010002",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/2",
abstract = "Biodiversity loss is a global problem, accelerated by
human-induced pressures. In the marine realm, one of
the major threats to species conservation, together
with climate change, is overfishing. In this context,
having information on the conservation status of target
commercial marine fish species becomes crucial for
assuring safe standards. We put together fisheries
statistics from the FAO, the IUCN Red List, FishBase,
and RAM Legacy databases to understand to what extent
top commercial species' conservation status has been
assessed. Levels of assessment for top-fished species
were higher than those for general commercial or highly
commercial species, but almost half of the species have
outdated assessments. We found no relation between IUCN
Red List traits and FishBase Vulnerability Index,
depreciating the latter value as a guidance for
extinction threat. The RAM database suggests good
management of more-threatened species in recent
decades, but more data are required to assess whether
the trend has reverted in recent years. Outdated IUCN
Red List assessments can benefit from reputed stock
assessments for new reassessments. The future of IUCN
Red List evaluations for commercial fish species relies
on integrating new parameters from fisheries sources
and improved collaboration with fisheries stakeholders
and managers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jia:2022:FDM,
author = "Hui Jia and Hui Zhang and Weiwei Xian",
title = "Fish Diversity Monitored by Environmental {DNA} in the
\geoname{Yangtze River} Mainstream",
journal = j-FISHES,
volume = "7",
number = "1",
pages = "1",
day = "22",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7010001",
ISSN = "2410-3888",
bibdate = "Sat Jan 22 12:17:28 MST 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/1/1",
abstract = "Surveys and assessments based on environmental DNA are
not only efficient and time-saving, but also cause less
harm to monitoring targets. Environmental DNA has
become a common tool for the assessment and monitoring
of aquatic organisms. In this study, we investigated
fish resources in the Yangtze River mainstream using
environmental DNA, and the variations in fish during
two seasons (spring and autumn) were compared. The
results showed that 13 species were identified in
spring, and nine species of fish were identified in
autumn. The fish with higher eDNA detection were
Sinibotia superciliaris, \bioname{Tachysurus
fulvidraco}, \bioname{Cyprinus carpio},
Ctenopharyngodon Idella, \bioname{Monopterus albus},
Acanthogobius hasta, Saurogobio dabryi,
\bioname{Oncorhynchus mykiss}, \bioname{Mugil
cephalus}, Odontamblyopus rubicundus. Seasonal
variation between spring and autumn was not
significant, and the environmental factors had
different effects on fish assemblages during the two
seasons. Our study used the eDNA technique to monitor
the composition of fish in the spring and autumn in the
Yangtze River mainstream, providing a new technology
for the long-term management and protection of fishery
resources in the region. Of course, problems such as
pollution and insufficient databases are the current
shortcomings of environmental DNA, which will be the
focus of our future research and study.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ferri:2022:AGU,
author = "Josipa Ferri and An{\dbar}ela Brzica",
title = "Age, Growth, and Utility of Otolith Morphometrics as
Predictors of Age in the \geoname{European} Barracuda,
\bioname{Sphyraena sphyraena} ({Sphyraenidae}):
Preliminary Data",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "68",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020068",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/68",
abstract = "Age and growth of the European barracuda, Sphyraena
sphyraena, were determined by examining sagittal
otoliths belonging to fish sampled in the eastern
Adriatic Sea, as the northernmost region of the
Mediterranean. A total of 113 specimens (59 females, 53
males, and one individual of indeterminate sex),
ranging from 23.4 to 42.5 cm in total length, were
analyzed. The maximum observed age was 5 years for both
females and males, and this barracuda population was
dominated by 3 year old fish. Growth was described by
the von Bertalanffy growth curve (L {\infty} = 55.58
cm, K = 0.12 year {$-$1}, t 0 = {$-$4}.29 year, R 2 =
0.580), and the growth performance index ({\Phi}') was
2.57. Otolith length, width, and mass were measured,
and the utility of these morphometrics as predictors of
age in S. sphyraena was evaluated. The results showed
that counting otolith annuli produced a better
estimation of age than proposed linear models based on
relationships between observed fish age and otolith
morphometrics. In comparison with age and growth data
available in the literature for S. sphyraena, our
results from the Adriatic Sea provide more insights
into the life-history traits of this species and can be
used in the future effective management and
conservation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{DiPaola:2022:SZE,
author = "Davide {Di Paola} and Carmelo Iaria and Giovanni
Lanteri and Marika Cordaro and Rosalia Crupi and
Rosalba Siracusa and Ramona D'Amico and Roberta Fusco
and Daniela Impellizzeri and Salvatore Cuzzocrea and
Nunziacarla Span{\`o} and Enrico Gugliandolo and
Alessio Filippo Peritore",
title = "Sensitivity of Zebrafish Embryogenesis to Risk of
Fotemustine Exposure",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "67",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020067",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/67",
abstract = "Several anthropogenic products in wastewater are
considered a threat to the aquatic environment. In
addition to common industrial pollutants, levels of
pharmaceuticals have been increasingly found in the
environment in recent years, which may present a strong
risk to the aquatic species that live there. The
constant consumption of biologically active chemicals
for human health has been matched by an increase in the
leaking of these compounds in natural habitats over the
last two decades. This study is aimed at evaluating the
developmental toxicity of fotemustine in the ecological
environment. Zebrafish embryos were exposed to doses of
25, 50 and 100 $ \mu $ g/mL from 4 h post-fertilization
to 120 h. This study confirms that fotemustine exposure
at 50 and 100 $ \mu $ g/mL affects the survival and
hatching rate, morphology score and body length.
Additionally, it significantly disturbs the antioxidant
defense system and increases ROS in zebrafish larvae.
From the molecular point of view, fotemustine exposure
strongly induces apoptosis, endoplasmic reticulum
stress (ERS) and the Wnt signaling pathway.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhu:2022:PSA,
author = "Yahua Zhu and Tao Jiang and Xiubao Chen and Hongbo Liu
and Quinton Phelps and Jian Yang",
title = "A Pilot Study Assessing a Concentration of 100 {mg/L}
Alizarin Complexone ({ALC}) to Mark Calcified
Structures in \bioname{Hypophthalmichthys molitrix}",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "66",
day = "16",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020066",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/66",
abstract = "The effectiveness of chemical compounds for marking
hard tissues in juvenile silver carp
(Hypophthalmichthys molitrix) is not well known. We
analyzed the use of alizarin complexone (ALC) as a
fluorescent marker to mark the various hard structures
of juvenile silver carp. Experimental fish ($ \approx
$2 months old) were randomly assigned to either control
or marking groups, which were immersed in 0 or 100 mg/L
ALC solutions, respectively, for 2 days. The otoliths,
fin rays, and scales of the fish were then sampled,
visualized using fluorescence microscopy, and evaluated
after 10 days. The ALC treatment was effective for
marking certain hard structures and the marking color
was affected by the light source. There were no obvious
differences in the marking efficiency of rays from
pectoral, dorsal, ventral, anal, and caudal fins, but
the lapilli and lateral line scales were marked most
effectively from the sampled otolith and scale types,
respectively. Our findings indicate that ALC immersion
and fin ray and scale sampling can be used for the
effective marking and non-lethal evaluation of hard
structures in juvenile silver carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Su:2022:CAI,
author = "Xin Su and Da Ji and Junjie Yao and Yuanlong Zou and
Mengzhi Yan",
title = "Comparative Analysis of Intestinal Characteristics of
Largemouth Bass (\bioname{Micropterus salmoides}) and
Intestinal Flora with Different Growth Rates",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "65",
day = "15",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020065",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/65",
abstract = "To explore the causes of different growth rates among
juvenile populations of largemouth bass, in the present
study, a batch of largemouth bass juveniles reared
under the same conditions were divided into a
fast-growing group and a slow-growing group. We used
histological, enzymatic and molecular biology methods
to analyze and determine their histomorphological
changes, digestive enzyme activity and intestinal
floral composition. The fast-growing group had a
significantly (p {\leq} 0.01) greater intestinal fold
height and muscle thickness than the slow-growing
group. Lipase activity was significantly (p {\leq}
0.01) higher in the fast-growing group than in the
slow-growing group. Intestinal microbial analysis
showed that the relative abundance of Actinobacteria
and Halomonas in the fast-growing group was higher than
that in the slow-growing group. This research shows
that the differentiation of growth rates in juvenile
largemouth bass populations is closely related to
intestinal fold status, lipase activity, and intestinal
flora.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2022:SDR,
author = "Zhicong Zhang and Fengyu Ji and Shouwen Jiang and
Zhichao Wu and Qianghua Xu",
title = "Scale Development-Related Genes Identified by
Transcriptome Analysis",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "64",
day = "12",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020064",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/64",
abstract = "Scales, as key structures of fish skin, play an
important role in physiological function. The study of
fish scale development mechanisms provides a basis for
exploring the molecular-level developmental differences
between scaled and non-scaled fishes. In this study,
alizarin red staining was used to divide the different
stages of zebrafish (Danio rerio) scale development.
Four developmental stages, namely stage I ($ \approx
$17 dpf, scales have not started to grow), stage II ($
\approx $33 dpf, the point at which scales start to
grow), stage III ($ \approx $41 dpf, the period in
which the scales almost cover the whole body), and
stage IV ($ \approx $3 mpf, scales cover the whole
body), were determined and used for subsequent
transcriptome analysis. WGCNA (weighted correlation
network analysis) and DEG (differentially expressed
gene) analysis were used for screening the key genes.
Based on the comparison between stage II and stage I,
54 hub-genes were identified by WGCNA analysis. Key
genes including the Scpp family (Scpp7, Scpp6, Scpp5,
and Scpp8), the Fgf family (Fgfr1b and Fgfr3), Tcf7,
Wnt10b, Runx2b, and Il2rb were identified by DEG
analysis, which indicated that these genes played
important roles in the key nodes of scale development
signal pathways. Combined with this analysis, the
TGF-{\beta}, Wnt/{\beta}-catenin, and FGF signaling
pathways were suggested to be the most important signal
pathways for scales starting to grow. This study laid a
foundation for exploring the scale development
mechanism of other fishes. The scale development
candidate genes identified in the current study will
facilitate functional gene identifications in the
future.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vaz:2022:EIM,
author = "Mariana Vaz and Damiana Pires and Pedro Pires and
Marco Sim{\~o}es and Ana Pombo and Paulo Santos and
Beatriz do Carmo and Ricardo Passos and Janina Z. Costa
and Kim D. Thompson and Teresa Baptista",
title = "Early Immune Modulation in \geoname{European} Seabass
(\bioname{Dicentrarchus labrax}) Juveniles in Response
to \bioname{Betanodavirus} Infection",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "63",
day = "11",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020063",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/63",
abstract = "The early host--pathogen interaction between European
seabass (Dicentrarchus labrax) and Betanodavirus was
examined by using juvenile fish infected
intramuscularly with RGNNV (red-spotted grouper nervous
necrosis virus). The time course selected for sampling
(0--144 h post-infection (hpi)) covered the early
stages of infection, with hematological, antioxidant
and immunological responses examined. Early activation
of the host's immune system was seen in the first few
hours post-infection (6 to 9 hpi), as evidenced by an
increase in tnf{\alpha}, cd28 and c3 expression in the
head kidney of infected fish. Most hematological
parameters that were examined showed significant
differences between sampling times, including
differences in the number of thrombocytes and various
leukocyte populations. The plasma lysozyme
concentration decreased significantly over the course
of the trial, and most antioxidant parameters examined
in the liver showed significant differences over the
infection period. At 144 hpi, peak expression of
tnf{\alpha} and il-1{\beta} coincided with the
appearance of disease symptoms, peak levels of virus in
the brain and high levels of fish mortality. The
results of the study show the importance of analyzing
the early interactions between European seabass and
Betanodavirus to establish early indicators of
infection to prevent more severe outcomes of the
infection from occurring.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tanaka:2022:BCO,
author = "Teruyoshi Tanaka and Tomoki Honryo and Yoshifumi
Sawada and Daniel Margulies and Vernon Scholey and
Jeanne Wexler and Maria Stein and Amal Biswas and Kenji
Takii",
title = "Biochemical Changes Occurring in Yellowfin Tuna Eggs
during Embryonic Development",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "62",
day = "11",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020062",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/62",
abstract = "Changes in nutritional constituents and enzyme
activities were clarified in yellowfin tuna (YFT,
Thunnus albacares) eggs during embryonic development,
from eggs immediately after fertilization to hatching.
The protein levels in the eggs gradually increased with
development until the completion of hatching. In
contrast, the triglyceride (TG) and free amino acid
(FAA) levels in the eggs gradually declined with
embryonic development until hatching was complete,
although the energy composition of the FAAs was lower
than that of the TGs throughout embryonic development.
These results indicate that endogenous TGs are
preferentially expended as an energy source during
embryonic development. Overall, changes in the
activities of aspartate aminotransferase, alanine
aminotransferase, creatine kinase, and alkaline
phosphatase showed similar patterns throughout
development. First, the enzyme levels diminished; then,
they remained at constant, low levels just before
hatching, when they rapidly increased. This rapid
increase was consistent with the protein content,
suggesting that organ differentiation and
functionalization were promoted during this period.
These results will contribute to the establishment of
mass-seeding production of YFT.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fang:2022:CAB,
author = "Di-An Fang and Kai Liu and Dong-Po Xu and Yin-Ping
Wang and Pao Xu",
title = "Comparative Analysis of Blood Transcriptome in the
{Yangtze} Finless Porpoise (\bioname{Neophocaena
asiaeorientalis})",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "61",
day = "10",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020061",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/61",
abstract = "The Yangtze finless porpoise (Neophocaena
asiaeorientalis) is the sole freshwater subspecies of
Neophocaena phocaenoides, and there is a lack of data
on its transcriptome. In this study, we applied RNA-seq
technology to assemble, de novo, a transcriptome and
analyzed differential expressed genes (DEGs). About 6
Gb of clean data was generated for the Yangtze finless
porpoise blood (n = 6) through de novo sequencing. In
total, 151,211 unigenes were generated and a total of
119,039 of these unigenes (78.72\%) were functionally
annotated when searched for within the NCBI Nr,
SwissProt, GO, COG, and KEGG databases. Diverse and
extensive expressed gene catalogs were sampled for the
Yangtze finless porpoise. DESeq2 was used to analyze
the differential expression genes (DEGs) obtained from
the assembled transcriptome. The results indicated that
DEGs have close relationships with the Yangtze finless
porpoise's development, evolution and adaptation.
Further, we found that genes involved in cetacean TAG
synthesis might directly explain the molecular basis of
cetacean blubber thickening. These transcriptome data
will assist in understanding molecular mechanisms of
Yangtze finless porpoise adaptation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ma:2022:CEP,
author = "Qian Ma and Jiehua Kuang and Gang Chen and Jiandong
Zhang and Jiansheng Huang and Feifan Mao and Qiling
Zhou",
title = "Cloning and Expression Profiling of the Gene
\gene{vasa} during First Annual Gonadal Development of
Cobia (\bioname{Rachycentron canadum})",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "60",
day = "10",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020060",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/60",
abstract = "The vasa gene is essential for germ cell development
and gametogenesis both in vertebrates and in
invertebrates. In the present study, vasa (Rcvasa) cDNA
was cloned from cobia (Rachycentron canadum) using the
RACE amplification method. We found that the
full-length cDNA sequence of Rcvasa comprises 2571 bp,
containing a 5{\prime}-UTR of 145 bp, a 3{\prime}-UTR
of 341 bp, and an open reading frame (ORF) of 2085 bp,
encoding a protein of 694 aa. The deduced amino acid
sequence contains 8 conserved motifs of the DEAD-box
protein family, 7 RGG repeats, and 10 RG repeats in the
N-terminal region. Comparisons of the deduced amino
acid sequence with those of other teleosts revealed the
highest percentage identity (86.0\%) with Seriola
quinqueradiata. By using semiquantitative RT-PCR,
Rcvasa appeared to be specifically expressed in the
testis and ovary, among 13 tissues analyzed. In
addition, annual changes in Rcvasa expression levels
were examined in the gonads by quantitative real-time
PCR (qRT-PCR). The expression of Rcvasa in the testis
first increased significantly at 120 dph (stage
II--III), then stabilized as the testis developed from
185 dph (stage III) to 360 dph (stage V). During the
development of the ovary (stage I to II), the
expression of Rcvasa first increased and reached the
highest level at 210 dph (stage II), then decreased.
Furthermore, the results of chromogenic in situ
hybridization (CISH) revealed that Rcvasa mRNA was
mainly expressed in germ cells and barely detected in
somatic cells. In the testis, Rcvasa mRNA signal was
concentrated in the periphery of spermatogonia, primary
spermatocytes, and secondary spermatocytes and was
significantly weaker in spermatids and spermatozoa. In
the ovary, Rcvasa mRNA signal was uniformly distributed
in the perinuclear cytoplasm and was intense in early
primary oocytes (stage I and II). These findings could
provide a reference for understanding the regulatory
mechanisms of vasa expression during the development of
germ cells in cobia.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Basto-Silva:2022:EDP,
author = "Catarina Basto-Silva and Irene Garc{\'\i}a-Meil{\'a}n
and Ana Couto and Cl{\'a}udia R. Serra and Paula Enes
and Aires Oliva-Teles and Encarnaci{\'o}n Capilla and
In{\^e}s Guerreiro",
title = "Effect of Dietary Plant Feedstuffs and Protein\slash
Carbohydrate Ratio on Gilthead Seabream
(\bioname{Sparus aurata}) Gut Health and
Functionality",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "59",
day = "07",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020059",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/59",
abstract = "This study investigated, for the first time, the
integrated effects of dietary protein source and
protein/carbohydrate (P/CH) ratio on gilthead seabream
gut histomorphology, microbiota composition, digestive
enzymes activity, and immunological and oxidative
stress-related gene expressions. Four isolipidic diets:
two fishmeal-based (FM) and two plant feedstuff
(PF)-based diets, with P/CH ratios of 50/10 or 40/20
each (FM-P50/CH10; FM-P40/CH20; PF-P50/CH10;
PF-P40/CH20), were tested. PF-based diets lead to more
histomorphological alterations than FM-based diets.
P/CH ratio had no relevant effect on gut
histomorphology. Gut mucosa of fish fed PF-based diets
presented a higher number of operational taxonomic
units, and richness and diversity indices, while the
P/CH ratio did not affect those parameters. The
{\alpha}-amylase activity was lower in fish fed with
PF-based diets and in fish fed the P40/CH20 diets.
Regarding the immune-related genes, only
cyclooxygenase-2 was affected, being higher in fish fed
the P50/CH10 diets than the P40/CH20 diets. Fish fed
the FM-based diets presented higher expression of
glutathione reductase and glutathione peroxidase, while
fish fed the P50/CH10 diet had higher expression of
superoxide dismutase. In conclusion, PF-based diets can
compromise gut absorptive and digestive metabolism, but
decreasing the dietary P/CH ratio had little effect on
the parameters measured.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lipej:2022:AAI,
author = "Lovrenc Lipej and Marcelo Kova{\v{c}}i{\'c} and Jakov
Dul{\v{c}}i{\'c}",
title = "An Analysis of \geoname{Adriatic}
Ichthyofauna---Ecology, Zoogeography, and Conservation
Status",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "58",
day = "04",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020058",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/58",
abstract = "The paper presents an analysis of biogeographic and
habitat distribution patterns, and the conservation
status data of Adriatic fishes, based on the last
published checklist and evidence-based critical
analyses of species presence. The total number of
species recorded in the Adriatic is 449. The Adriatic
has 58.8\% of Mediterranean species richness, 76.1\% of
its families, and 87.8\% of its orders. Among species
discovered in the Adriatic after 2010, twelve species
were attributed to biological invasion, mostly Atlantic
immigrants or alien species, and ten species were
attributed to improved research on the native
ichthyofauna of the Adriatic area. About 58\% of
species are native species of Atlanto-Mediterranean
origin, 21\% are native species of wider global
occurrence, 15\% are Mediterranean or Mediterranean and
Black Sea endemics and 5\% originated outside
Mediterranean Sea. The majority of species inhabit the
benthic environment (71.9\%), while others occur in the
pelagic environment (20.7\%) or are euryhaline (7.3\%).
The benthic littoral species are the most numerous
Adriatic fishes, representing 40\% of all species
richness, whereas pelagic fishes are mainly eurybathic
or epipelagic; only 3.6\% of species are deep pelagic
species. A Red Book of marine fishes of the Adriatic
Sea is urgently needed to assess their conservation
status, covering the entire Adriatic Sea and reviewing
all fish species to assess their conservation status.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gao:2022:GMF,
author = "Minghui Gao and Zhiqiang Wu and Liangliang Huang and
Xichang Tan and Mingsi Li and Haibo Huang",
title = "Growth and Microstructural Features in Otoliths of
Larval and Juvenile \bioname{Sinogastromyzon wui} ({F.
Balitoridae, River Loaches}) of the {Upper Pearl River,
China}",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "57",
day = "01",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020057",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/57",
abstract = "Otolith growth and microstructural features of fish
are essential to the understanding of the early fish
lifecycle. This paper assesses the features of otoliths
from laboratory-reared larval and juvenile
Sinogastromyzon wui (S. wui, 0 to 25 days
post-hatching) that were obtained as eggs from the
Shilong Reach of Xijiang River between April and August
2021. We observed the development of the three pairs of
otoliths (lapilli, sagittae, and asterisci) and
compared the shape changes and growth of the lapilli
and sagittae, as well as the timing and deposition rate
of increments of the lapilli. The lapilli and the
sagittae were visible on hatching, whereas the
asterisci were present at four days post-hatching
(dph). The shape of the sagitta changed more obviously
than that of the lapillus, and a strong correlation was
observed between sagitta shape changes and fish
ontogenesis. The otolith shape greatly modulated during
the post-flexion larval stage (Post-FLS), it
corresponded with the formation period of individual
fins. Analysis of the microstructural features
indicated that lapilli were the optimal otolith for age
determination and increment deposition rate
confirmation. Using regression analysis of the known
age and the number of lapillus daily increments, we
demonstrated that the lapillus developmental increments
were deposited daily, and the first increment formed at
two days post-hatching. Our conclusions support
employing the lapillus increment deposition rate and
the time of the first daily increments in the
determination of the age of wild larval and juvenile S.
wui.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zemah-Shamir:2022:SRB,
author = "Ziv Zemah-Shamir and Shiri Zemah-Shamir and Aviad
Scheinin and Dan Tchernov and Teddy Lazebnik and Gideon
Gal",
title = "A Systematic Review of the Behavioural Changes and
Physiological Adjustments of Elasmobranchs and
Teleost's to Ocean Acidification with a Focus on
Sharks",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "56",
day = "28",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020056",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/56",
abstract = "In recent years, much attention has been focused on
the impact of climate change, particularly via ocean
acidification (OA), on marine organisms. Studying the
impact of OA on long-living organisms, such as sharks,
is especially challenging. When the ocean waters absorb
anthropogenic carbon dioxide (CO 2), slow-growing shark
species with long generation times may be subjected to
stress, leading to a decrease in functionality. Our
goal was to examine the behavioral and physiological
responses of sharks to OA and the possible impacts on
their fitness and resilience. We conducted a systematic
review in line with PRISMA-Analyses, of previously
reported scientific experiments. We found that most
studies used CO 2 partial pressures (pCO 2) that
reflect representative concentration pathways for the
year 2100 (e.g., pH $ \approx $7.8, pCO 2 $ \approx
$1000 {\mu}atm). Since there is a considerable
knowledge gap on the effect of OA on sharks, we
utilized existing data on bony fish to synthesize the
available knowledge. Given the similarities between the
behaviors and physiology of these two superclasses' to
changes in CO 2 and pH levels, there is merit in
including the available information on bony fish as
well. Several studies indicated a decrease in shark
fitness in relation to increased OA and CO 2 levels.
However, the decrease was species-specific and
influenced by the intensity of the change in
atmospheric CO 2 concentration and other anthropogenic
and environmental factors (e.g., fishing, temperature).
Most studies involved only limited exposure to future
environmental conditions and were conducted on benthic
shark species studied in the laboratory rather than on
apex predator species. While knowledge gaps exist, and
more research is required, we conclude that
anthropogenic factors are likely contributing to shark
species' vulnerability worldwide. However, the impact
of OA on the long-term stability of shark populations
is not unequivocal.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lobel:2022:DEB,
author = "Lisa Kerr Lobel and Phillip S. Lobel",
title = "Damselfish Embryos as a Bioindicator for Military
Contamination on Coral Reefs at \geoname{Johnston
Atoll, Pacific Ocean}",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "55",
day = "26",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020055",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/55",
abstract = "This study investigated the association between
sediment contamination, PCB accumulation in adult
nesting males and the occurrence of embryonic
abnormalities in the damselfish, Abudefduf sordidus,
from two sites with high PCB contamination and three
``reference'' sites (contaminants very low or not
measurable) within Johnston Atoll, Central Pacific
Ocean. Developmental abnormalities were assessed in
damselfish embryos collected in the field during four
natural spawning seasons (1996, 1998, 1999, and 2001).
Laboratory incubations of abnormal embryos demonstrated
that the observed abnormalities were lethal. PCBs were
measured in fish collected in three years. Mean
whole-body concentrations of total PCBs ranged from
364.6 to 138,032.5 ng/g lipid. A significant
residue--effect relationship was found between total
PCB concentration and embryo abnormalities. The
occurrence of embryo abnormalities was positively
related to fish PCB concentration (other contaminants
were also evaluated including metals and dioxins). This
study demonstrates the utility of using damselfish
embryos as a bioindicator tool for monitoring coral
reefs. It also provides baseline-monitoring criteria
and evaluates sediment quality benchmarks used for
ecological risk assessments on coral reefs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2022:ERD,
author = "Qian Xu and Zheng Yang and Siyu Chen and Wenjuan Zhu
and Siyuan Xiao and Jing Liu and Hongquan Wang and
Shile Lan",
title = "Effects of Replacing Dietary Fish Meal by Soybean Meal
Co-Fermented Using \bioname{Bacillus subtilis} and
\bioname{Enterococcus faecium} on Serum Antioxidant
Indices and Gut Microbiota of Crucian Carp
\bioname{Carassius auratus}",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "54",
day = "25",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020054",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Xu:2023:CXA}.",
URL = "https://www.mdpi.com/2410-3888/7/2/54",
abstract = "Fermented soybean meal (FSM) is an important feed
material that can replace fish meal to solve the
shortage of animal protein. To improve the utilization
of FSM, we optimized the co-fermentation conditions of
soybean meal using Bacillus subtilis and Enterococcus
faecium and studied the effects of replacing fish meal
with different proportions of FSM on serum antioxidant
indices and gut microbiota (GM) composition of crucian
carp (Carassius auratus). Our results showed that the
co-fermentation of soybean meal was the most effective
when the ratio of B. subtilis X-2 and E. faecium X-4
was 2:3, glucose addition was 4.5\%, KH 2 PO 4 addition
was 0.15\%, MgSO 4 {\cdot}7H 2 O addition was 0.1\%,
anhydrous sodium acetate addition was 0.4\%,
fermentation time was 120 h, and the solid--water ratio
was 1:1. Replacing 40\% fish meal with FSM in the feed
significantly improved the serum T-AOC, POD, and IgM
levels in C. auratus. Although there were significant
differences in the midgut and hindgut microbiota
structures of C. auratus, the addition of FSM to the
feed did not cause significant differences in the GM
structure, whether in the midgut or hindgut. Therefore,
40\% FSM is the most suitable substitute for fish meal
in the feed of C. auratus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Blanco:2022:ARE,
author = "Miguel Blanco and Lydia Bares and Marcos Ferasso",
title = "Analysis of Regional Efficiency in the Use of the
{European Maritime and Fisheries Fund (FEMP)} in the
{Eurozone} Countries",
journal = j-FISHES,
volume = "7",
number = "2",
pages = "53",
day = "22",
month = apr,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7020053",
ISSN = "2410-3888",
bibdate = "Thu Mar 17 11:56:42 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/2/53",
abstract = "The European Union (EU) launched the Common Fisheries
Policy (CFP) in the 1970s. Currently, a large part of
the CFP has been financed with the European Maritime
and Fisheries Fund (FEMP). This research aims to
determine whether there is a high degree of homogeneity
in the levels of efficiency achieved by the fishing
projects financed by European Funds for the period 2014
to 2020. The Data Envelopment Analysis (DEA)
methodology was adopted. The main results showed that
there is a high degree of heterogeneity in the level of
efficiency achieved by European countries during the
analyzed period. Moreover, despite the fact that
regional efficiency has been increasing during the
years under study, territorial disparities persist over
time. This research provides a contribution for
policymakers targeting better use of the FEMP funds for
the upcoming funding package 2021--2027.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Peixoto:2022:TFF,
author = "Ualerson I. Peixoto and Adauto S. Mello-Filho and
Bianca Bentes and Victoria J. Isaac",
title = "Trawl Fishing Fleet Operations Used to Illustrate the
Life Cycle of the Southern Brown Shrimp: Insights to
Management and Sustainable Fisheries",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "141",
day = "17",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030141",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/141",
abstract = "The purpose of this study was to better understand the
life cycle of brown shrimp along the Amazon Continental
Shelf by using spatial and temporal trawl fleet
activities. A total of 208,121 specimens and 1281
trawls were studied throughout the course of 13 years
of shrimp size composition. To investigate differences
in length composition between fishing grounds, months,
and depth, a PERMANOVA analysis was employed. A
geographic information system was developed for
environmental characterisation and spatiotemporal trawl
fleet distribution. Our findings show that the
industrial trawl shrimp fleet has a close relationship
with shrimp biological characteristics, following
shrimp migration patterns in different months,
locations, and depths during different stages of their
life cycle, and that this fleet acts on two-yearly
cohorts. The management measures of limiting effort
(number of vessels) appear enough to avoid
overcapitalisation, but the closed period and a
proposed no-take fishing zone appear insufficient for
what was originally proposed. Ecosystem-based
management strategies should be addressed immediately
because they would be far more effective than
traditional fishery-based management measures in
promoting sustainable fishing.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tran:2022:CFW,
author = "Thi Ngoc Tran and Chien Thang Doan and Van Bon Nguyen
and Anh Dzung Nguyen and San-Lang Wang",
title = "Conversion of Fishery Waste to Proteases by
\bioname{Streptomyces speibonae} and Their Application
in Antioxidant Preparation",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "140",
day = "14",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030140",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/140",
abstract = "Proteinaceous wastes from the fishery process are an
abundant renewable resource for the recovery of a
variety of high-value products. This work attempted to
utilize several proteinaceous wastes to produce
proteases using the Streptomyces speibonae TKU048
strain. Among different possible carbon and nitrogen
sources, the protease productive activity of S.
speibonae TKU048 was optimal on 1\% tuna head powder.
Further, the casein/gelatin/tuna head powder zymography
of the crude enzyme revealed the presence of
three/nine/six proteases, respectively. The
crude-enzyme cocktail of S. speibonae TKU048 exhibited
the best proteolytic activity at 70 {$^\circ $}C and pH
= 5.8. Sodium dodecyl sulfate strongly enhanced the
proteolytic activity of the cocktail, whereas FeCl 3,
CuSO 4, and ethylenediaminetetraacetic acid could
completely inhibit the enzyme activity. Additionally,
the crude-enzyme cocktail of S. speibonae TKU048 could
efficiently enhance the 2,2-diphenyl-1-picrylhydrazyl
and
2,2{\prime}-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) radical scavenging activities of all tested
proteinaceous materials including the head, viscera,
and meat of tuna fish; the head, viscera, and meat of
tilapia fish; the head, meat, and shell of shrimp;
squid pen; crab shell; and soybean. Taken together, S.
speibonae TKU048 revealed potential in the reclamation
of proteinaceous wastes for protease production and
antioxidant preparation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2022:MCH,
author = "Jianlu Zhang and Jiqin Huang and Cheng Fang and
Wanchun Li and Hu Zhao and Fei Kong and Han Zhang and
Hongxing Zhang and Qijun Wang",
title = "Molecular Cloning of Heat Shock Protein 60 ({Sp
HSP60}) from \bioname{Schizothorax prenanti} and the
Gene Expressions of Four {Sp HSPs} during
Lipopolysaccharide ({LPS}) Infection",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "139",
day = "13",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030139",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/139",
abstract = "Heat shock proteins (HSPs) play a key role in
anti-stress and immune processes and are associated
with autoimmune diseases. In order to explore the
immunological role of HSPs from Schizothorax prenanti
(S. prenanti), Sp HSP60 was cloned for the first time
in this study, and the gene expressions of Sp HSP27, Sp
HSP60, Sp HSP70 and Sp HSP90 in the hepatopancreas,
head kidney, hindgut and spleen were analyzed by
quantitative real-time PCR (qPCR) after treatment with
lipopolysaccharide (LPS). The open reading frame of the
Sp HSP60 gene (GenBank accession number ON245159) is
1728 bp. It encodes a protein of 575 amino acids. Its
C-terminus is a highly conserved and repeated glycine
sequence, which is an important cofactor in ATP
binding. Compared with the control group, most of the
Sp HSPs were significantly upregulated in the tissues
examined at 12 or 24 h after LPS challenge. The most
abundant expression of Sp HSP70 was found in the head
kidney at 24 h after LPS injection, followed by Sp
HSP27 in the spleen at 24 h; both of these Sp HSPs
displayed strong expression under the LPS stresses,
about 20--70 fold more than that of Sp HSP60 and Sp
HSP90. The temporal expression patterns of the four Sp
HSP genes were different in the four tissues examined.
Taken together, the results suggest that Sp HSP27, Sp
HSP60, Sp HSP70 and Sp HSP90 participate in innate
immunity stimulated by LPS, and the response intensity
of the Sp HSPs was organ-specific, indicating they
could provide early warning information against
bacterial infection. The findings in our study will
contribute to better understanding the biological
processes and important roles of Sp HSPs involved in
defending against pathogenic bacterial challenge.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jin:2022:PAE,
author = "Jiali Jin and Zhipeng Chu and Rui Ruan and Wei Liu and
Xihua Chen and Chuangju Li",
title = "Phosphorus Absorption and Excretion in Hybrid Sturgeon
(\bioname{Huso dauricus} [female] {X}
\bioname{Acipenser schrenckii} [male]) Intubated with
Different {Ca/P} Ratios",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "138",
day = "10",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030138",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/138",
abstract = "To study the effect of Ca/P ratio on the P and Ca
absorption and excretion in hybrid sturgeon (Huso
dauricus {[female-sign]} X Acipenser schrenckii
{[male-sign]}), five groups of fish were intubated with
100 mg P{\cdot}kg {$-$1} BW with the Ca/P ratios of
0:1, 0.25:1, 0.5:1, 1:1, and 2:1. Plasma P
concentrations were significantly elevated at Ca/P
ratios below 2:1, and the highest value was obtained at
Ca/P ratio of 0.5:1. Plasma Ca content was
significantly increased at the highest Ca/P ratio.
Urine P excretion rate in the fish intubated with Ca/P
ratio of 0.5:1 was significantly higher than that of
the groups with Ca/P ratios of 0:1 and 2:1. The highest
urea excretion rates were observed at Ca/P ratio of
0.5:1 and 1:1. The total P excretion at 48 h post
intubation reached about 30 mg{\cdot}kg {$-$1} BW,
which was recorded for the group with Ca/P ratio of
0.5:1. The present study showed that P absorption
efficiency was improved in hybrid sturgeon at Ca/P
ratio of 0.5:1, indicating that P inclusion level in
sturgeon feed can be further optimized to reduce
dietary P input and lower the excessive undigested P
discharge into the rearing water.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Perez-Jimenez:2022:IFD,
author = "Graciela M. P{\'e}rez-Jim{\'e}nez and Emyr Saul
Pe{\~n}a-Mar{\'\i}n and Claudia I. Maytorena-Verdugo
and Cesar Antonio Sep{\'u}lveda-Quiroz and Luis Daniel
Jim{\'e}nez-Mart{\'\i}nez and Susana {De la
Rosa-Garc{\'\i}a} and Gloria Gertrudys Asencio-Alcudia
and Rafael Mart{\'\i}nez and Dariel Tovar-Ram{\'\i}rez
and Mario A. Galaviz and Talhia Mart{\'\i}nez-Burguete
and Carlos A. Alvarez-Gonz{\'a}lez and Carina Shianya
Alvarez-Villagomez",
title = "Incorporation of Fructooligosaccharides in Diets
Influence Growth Performance, Digestive Enzyme
Activity, and Expression of Intestinal Barrier Function
Genes in Tropical Gar (\bioname{Atractosteus tropicus})
Larvae",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "137",
day = "10",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030137",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/137",
abstract = "This study was conducted to investigate the effects of
dietary fructooligosaccharides (FOS) on the growth,
survival rate, digestive enzyms activity, and the
expression of intestinal barrier function genes in
tropical gar (Atractosteus tropicus) larvae. A total of
960 larvae (0.030 {\pm} 0.006 g) were fed three diets
supplemented with increasing FOS concentrations (2.5,
5, and 7.5 g kg {$-$1}) and a control diet for 15 days.
Results revealed that a 7.5 g kg {$-$1} FOS
supplementation improved weight gain, specific growth
rate, and survival rate (p {$<$} 0.05). Furthermore, 5
g kg {$-$1} FOS supplementation increased alkaline
protease and amylase activities and induced an
upregulation of the claudin-17 gene expression (p {$<$}
0.05). Meanwhile, the inclusion of 7.5 g kg {$-$1} FOS
induced the upregulation of mucin 2 (muc-2), and the
tight junction genes zo-2 and claudin-3 (p {$<$} 0.05).
In addition, 2.5, 5, and 7.5 g kg {$-$1} FOS promoted
the downregulation of the claudin-15 gene expression (p
{$<$} 0.05). At the same time, FOS inclusion did not
increase the pro-inflammatory cytokine il-8 expression.
We can conclude that 7.5 g kg {$-$1} FOS
supplementation improves growth performance, survival
rate, and digestive capacity, and could contribute to
the reinforcement of the intestinal barrier function of
Tropical gar larvae.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Falsone:2022:ASD,
author = "Fabio Falsone and Vita Gancitano and Michele Luca
Geraci and Giacomo Sardo and Danilo Scannella and
Fabrizio Serena and Sergio Vitale and Fabio
Fiorentino",
title = "Assessing the Stock Dynamics of
\bioname{Elasmobranchii} off the \geoname{Southern
Coast of Sicily} by Using Trawl Survey Data",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "136",
day = "07",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030136",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/136",
abstract = "Elasmobranchii (sharks and rays), which have peculiar
and vulnerable life-history traits, are highly
threatened by fishing activities. Indeed, between 53\%
and 71\% of Mediterranean elasmobranch species are at
risk of extinction. In this context, using the
abundance MSY (AMSY) model, the present study provides
an assessment of 20 batoids and 16 shark species in the
Strait of Sicily, sampled during a bottom trawl survey
from 1995 to 2020. Overall, the outputs underline a
progressively improving condition for shark and ray
assemblages of both shelf and eurybathic zones. As for
slope-dwelling species, a horseshoe-shaped dynamic,
characterized by a progressive decrease in relative
harvesting pressure and an increase in relative biomass
followed by an increase in fishing pressure and
decrease in biomass, was detected. The dynamics of the
Elasmobranchii living in the Strait of Sicily appear to
be affected by changes in the fishing patterns of
trawlers, showing a shift from shallow water to bathyal
fishing grounds and targeting deep-water red shrimp. In
this context, it seems wise to limit the impact of
deep-water fisheries on Elasmobranchii by reducing
fishing efforts and implementing ad hoc management
measures aimed at safeguarding these vulnerable
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Moura:2022:RPS,
author = "Ana Moura and Ester Dias and Rodrigo L{\'o}pez and
Carlos Antunes",
title = "Regional Population Structure of the
\geoname{European} Eel at the Southern Limit of Its
Distribution Revealed by Otolith Shape Signature",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "135",
day = "07",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030135",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/135",
abstract = "Given the European eel population's marked decrease
since the 1980s, it has become urgent to collect
information describing its regional population
structure to improve management plans. The Minho River
(NW-Portugal, SW-Europe) is an important basin for the
eel at the southern limit of its distribution, but the
species is poorly described. Thus, we aimed to study
the structure of the European eel population in the
Minho River using otolith shape analysis, which has
proven to be effective in discriminating fish groups
experiencing different environmental conditions through
ontogeny. Our results showed complete discrimination
between the two main types of habitats studied
(tributaries and estuaries). Otoliths of eels from the
estuary were rectangular and elliptic, whereas in the
tributaries they presented a more round and circular
form. Eels collected in both habitats were mostly
yellow-stage eels with a similar age range, but the
eels from the tributaries showed smaller length-at-age
and lower body condition than those collected in the
estuary. Additionally, the sex ratio was skewed towards
males in the tributaries and females in the estuary.
This study reveals that there are at least two distinct
groups of eels in this basin, likely with different
development characteristics.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yi:2022:SAM,
author = "Hong Yi and Qinglu Bai and Ying Li and Honglei Zhan
and Yujia Liu and Bingnan Liu and Jihui Wang",
title = "Sporosarcina aquimarina {MS4} Regulates the Digestive
Enzyme Activities, Body Wall Nutrients, Gut Microbiota,
and Metabolites of \bioname{Apostichopus japonicus}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "134",
day = "07",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030134",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/134",
abstract = "Sporosarcina aquimarina MS4 is a microecological
preparation for overwintering Apostichopus japonicus,
which has an immune regulation function, but its role
in the nutritional regulation of A. japonicus is not
clear. This study aimed to describe the effects of S.
aquimarina MS4 on the growth, digestion, and body wall
nutrition of A. japonicus through feeding experiments
and to discuss the potential mechanism of S. aquimarina
MS4 regulating gut function through the detection of
gut microbiota and metabolites. After 60 days of
culture, the growth performance of A. japonicus fed S.
aquimarina MS4 (10 8 cfu/g) significantly improved, and
the content of polysaccharide, leucine, phenylalanine,
lysine, and docosahexaenoic acid in the body wall
significantly increased. Gut microbiota analysis showed
that although Proteobacteria, Verrucomicrobia,
Firmicutes, and Bacteroidetes were the predominant
phyla in all the sea cucumbers, Haloferula and
Rubritalea showed significant difference between the
group fed with or without S. aquimarina MS4.
Metabolomics analysis showed that differential
metabolites in the gut were mainly enriched in amino
acid metabolism and lipid metabolism. The association
analysis of differential metabolites and microbiota
showed that the production of some differential
metabolites was significantly related to differential
microorganisms, which improved the understanding of the
function of microorganisms and their roles in the gut
of A. japonicus. This study reveals the life activities
such as growth and metabolism of A. japonicus, and it
provides support for the functional study of the gut
microbiome of A. japonicus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Junior:2022:UCE,
author = "Guerino Bandeira Junior and Adriane Erbice Bianchini
and Carine de Freitas Souza and Sharine Nunes Descovi
and Liana da Silva Fernandes and Lenise de Lima Silva
and Juliana Felipetto Cargnelutti and Bernardo
Baldisserotto",
title = "The Use of Cinnamon Essential Oils in Aquaculture:
Antibacterial, Anesthetic, Growth-Promoting, and
Antioxidant Effects",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "133",
day = "06",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030133",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/133",
abstract = "Cinnamon essential oils (EOs) are widely known for
their pharmaceutical properties; however, studies
investigating the use of these EOs in aquaculture are
scarce. The aims of this study were to evaluate the
anesthetic effect of bathing silver catfish (Rhamdia
quelen) with Cinnamomum cassia EO (CCEO) and its
nanoemulsion (NCCEO); the growth-promoting and
antioxidant effects of dietary supplementation with
CCEO in silver catfish; and the in vitro antibacterial
effect of CCEO, NCCEO, and Cinnamomum zeylanicum EO
(CZEO) against bacteria isolated from diseased silver
catfish. The two cinnamon EOs showed promising
antibacterial activity, which was potentiated by the
nanoemulsion. CCEO showed satisfactory anesthetic
activity in silver catfish, and its nanoemulsion
intensified the sedative activity. Supplementation of
1.0 mL CCEO per kg of diet for 60 days increased
weight, length, and weight gain when compared to the
control group, evidencing the growth-promoting activity
of this EO. Dietary supplementation of CCEO for 30 and
60 days also showed an antioxidant effect, as it
decreased levels of thiobarbituric acid reactive
species and increased the superoxide dismutase activity
in the liver of silver catfish. Therefore, cinnamon EOs
have a promising use in aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Durazzo:2022:NBC,
author = "Alessandra Durazzo and Gabriella {Di Lena} and Paolo
Gabrielli and Antonello Santini and Ginevra
Lombardi-Boccia and Massimo Lucarini",
title = "Nutrients and Bioactive Compounds in Seafood:
Quantitative Literature Research Analysis",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "132",
day = "05",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030132",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/132",
abstract = "This perspective presents current and updated advances
in research on nutrients and bioactive compounds in
seafood. It is based on a literature quantitative
research analysis approach. The main features of
seafood components are introduced. This perspective
aims at providing a current framework that relates
nutrients, bioactive compounds, and seafood in a novel
integrated and multidisciplinary manner, highlighting
the current knowledge, the main research lines, and
emerging strategies. The literature search was carried
out by means of the Scopus database, and 22,542
documents were retrieved in the period from 1932 to
2024. Particularly, from the perspective of nutrition
and health outputs, the main terms correlated with
research on the relationship between seafood and
nutritional and bioactive components, and the main
existing research lines focused on this topic, were
identified. The top recurring keywords were human/s,
female, diet, nutrition, fish, male, adult, food
intake.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Muringai:2022:SSA,
author = "Rodney Tatenda Muringai and Paramu Mafongoya and
Romano Trent Lottering",
title = "{Sub-Saharan Africa} Freshwater Fisheries under
Climate Change: a Review of Impacts, Adaptation, and
Mitigation Measures",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "131",
day = "02",
month = jun,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030131",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/131",
abstract = "Sub-Saharan Africa's freshwater fisheries contribute
significantly to the livelihoods and food security of
millions of people within the region. However,
freshwater fisheries are experiencing multiple
anthropogenic stressors such as overfishing, illegal
fishing, pollution, and climate change. There is a
substantial body of literature on the effects of
climate change on freshwater fisheries in Sub-Saharan
Africa. This study reviews the existing literature and
highlights the effects of climate change on freshwater
fisheries, the adaptation strategies of
fishery-dependent households in response to the
effects, and fisheries' management and mitigation
efforts in the face of climate change. The general
effects of climate change on freshwater environments
include warming water temperatures, increased
stratification, modified hydrological processes, and
increased pollutants. These effects adversely affect
the physiological processes of fish and the overall
wellbeing of fishery-dependent people. To cope with the
effects of fluctuating fishery resources due to climate
change, fishery-dependent people have adopted several
adaptation strategies including livelihood
diversification, changing their fishing gear,
increasing their fishing efforts, and targeting new
species. Several management attempts have been made to
enhance the sustainability of fishery resources, from
local to regional levels. This study recommends the
participation of the resource users in the formulation
of policies aimed at promoting climate change
adaptation and the resilience of freshwater fisheries
for sustainable development.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yu:2022:CGC,
author = "Hailuo Yu and Yi Liu and Tiaoyi Xiao and Hongquan Wang
and Baohong Xu",
title = "Cloning of Grass Carp Chemokine {XC} Receptor 1
({XCR1}) Gene and Evaluation of Its Expression in
Various Organs after {GCRV} Infection",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "130",
day = "31",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030130",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/130",
abstract = "Interaction between the chemokine receptor XCR1 and
its ligand is closely related to the immune function in
animals; however, there are only a few reports on role
of XCR1 in the immune system of fish. We aimed to
analyze the expression of XCR1 in various organs or
tissues of grass carp before and after Grass Carp
Reovirus (GCRV) infection to better understand the
function of XCR1 in resistance to GCRV infection. We
cloned and sequenced the cDNA of grass carp XCR1 and
analyzed the molecular structure of XCR1 based its
amino acid sequence. Further, we analyzed the relative
expression levels of XCR1 in different organs or
tissues of male parent grass carp with GCRV resistance
(P1) and their first-generation offspring (F1) before
and after GCRV infection. Our results show that the
total length of cDNA of the grass carp XCR1 gene is
1659 bp and encodes 365 amino acids. XCR1 contains
seven conserved transmembrane helical domains. The
homologous tertiary structure of XCR1 is similar to its
homologs in other species. After artificial GCRV
infection, there were significant differences in the
expression of the grass carp XCR1 gene in different
tissues, at different time points, and between P1 and
F1 fish. These results will contribute to our
understanding of the role of XCR1 in fish immune
responses and contribute to the development of
GCRV-resistant grass carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2022:MFT,
author = "Haoran Zhang and Kun Li and Fayang Zhang and Jikui Wu
and Junling Zhang",
title = "The {miR-200} Family Targeting amh Affects the Gonadal
Development of \geoname{Japanese} Flounder",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "129",
day = "31",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030129",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/129",
abstract = "Four members of the miR-200 family in Japanese
flounder (Paralichthys olivaceus) have sex-biased
expression patterns, but their target genes and how
they work in the development of the gonads are rarely
known. Anti-M{\"u}llerian hormone (AMH) can inhibit the
development of Muller's duct in female mammals and
regulate the formation of gametes after sexual
maturity. There is no Muller's duct in teleosts, but
the amh gene still exists. Knockout of amh results in
sex reversal from male to female. Therefore, it is
essential to explore the relationship between the
miR-200 family and amh to clarify what role miR-200
plays in the development of the gonads. In Japanese
flounder, the two binding sites for the miR-200 family
in the 3{\prime}UTR of amh were found through
bioinformatic prediction. Double luciferase and green
fluorescent protein reporter experiments demonstrated
amh to be directly targeted by miR-200a and miR-200b.
Moreover, miR-200a and miR-200b reduced the expression
of amh through site 1 rather than site 2. To explore
the regulatory role of miR-200a in gonadal development,
we further overexpressed miR-200a in the primary
Sertoli cells of the testis. With the overexpression of
miR-200a, the expression of amh decreased, while the
expression of the other two male sex-related genes,
dmrt1 (doublesex and mab-3 related transcription factor
1) and gsdf (diagonal soma driven factor), increased
significantly. This result indicates that the miR-200
family regulates the gonadal differentiation and
development by targeting amh in Japanese flounder.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2022:TAJ,
author = "Hongbo Liu and Junren Xue and Jing Tang and Tao Jiang
and Xiubao Chen and Jian Yang",
title = "Taste Attributes of the {``June Hairy Crab''}
Juveniles of \geoname{Chinese} Mitten Crab
(\bioname{Eriocheir sinensis}) in {Yangcheng Lake,
China}--- a Pilot Study",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "128",
day = "31",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030128",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/128",
abstract = "This is the first report on the use of a taste sensing
system to quantitatively evaluate the taste attributes
of two groups of native ``June hairy crab'' juveniles
(commonly referred to as ``Liu-Yue-Huang'') of the
Chinese mitten crab (Eriocheir Sinensis H. Milne
Edwards, 1853) from a net enclosure culture area in
Yangcheng Lake (lake culture) and aquaculture ponds
near the lake (pond culture). We showed that umami was
the predominant basic taste of steamed June hairy
crabs, followed by bitterness and astringency. The
intensity value of saltiness was aberrant and could not
be determined using this system. The average values of
aftertaste-U reached 8.7 and 10.7 in the male June
hairy crabs from the lake and pond cultures,
respectively, which was significantly higher than their
respective aftertaste-B and aftertaste-A values (p
{$<$} 0.01). Female crabs did not have aftertaste-B,
while their aftertaste-U was significantly higher than
aftertaste-A (p {$<$} 0.01). Although principal
component analysis and linear discriminant analysis
were not able to completely distinguish among crabs
from different cultures, they could robustly
distinguish between male and female crabs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Maytorena-Verdugo:2022:IMO,
author = "Claudia I. Maytorena-Verdugo and Emyr S.
Pe{\~n}a-Mar{\'\i}n and Carina S.
Alvarez-Villag{\'o}mez and Graciela M.
P{\'e}rez-Jim{\'e}nez and C{\'e}sar A.
Sep{\'u}lveda-Quiroz and Carlos A.
Alvarez-Gonz{\'a}lez",
title = "Inclusion of Mannan-Oligosaccharides in Diets for
Tropical Gar \bioname{Atractosteus tropicus} Larvae:
Effects on Growth, Digestive Enzymes, and Expression of
Intestinal Barrier Genes",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "127",
day = "31",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030127",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/127",
abstract = "Mannan-oligosaccharides (MOS) are non-digestible
carbohydrates, and their use in aquaculture as
prebiotics is well documented. The objective of this
work was to test whether MOS supplemented in the diet
of A. tropicus larvae (2, 4, and 6 g kg {$-$1})
influence growth parameters, the activity of digestive
enzymes, and the expression of genes related to the
intestinal barrier. The highest total length was
observed in larvae fed 6 g kg {$-$1} MOS compared to
control larvae. Trypsin activity increased with the
addition of MOS to the diets, but leucine
aminopeptidase activity only increased with 6 g kg
{$-$1} MOS. Lipase and {\alpha}-amylase activities
increased in larvae fed with 2 and 4 g kg {$-$1} MOS.
The expression of zo-2 was higher with the 6 g kg
{$-$1} MOS treatment. The cl-3 transcripts were lower
with 2 g kg {$-$1} MOS but higher with 6 g kg {$-$1}
MOS. All tested concentrations of MOS increased the
expression of muc-2. In this study, incorporating
mannan-oligosaccharides into the diet of A. tropicus
larvae had a positive effect, and the concentration of
6 g kg {$-$1} produced the best results. Therefore,
including this prebiotic in the diets for the culture
of A. tropicus larvae is suitable.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Arechavala-Lopez:2022:SSR,
author = "Pablo Arechavala-Lopez and Samira Nu{\~n}ez-Velazquez
and Carlos Diaz-Gil and Guillermo Follana-Bern{\'a} and
Jo{\~a}o L. Saraiva",
title = "Suspended Structures Reduce Variability of Group
Risk-Taking Responses of \bioname{Dicentrarchus labrax}
Juvenile Reared in Tanks",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "126",
day = "31",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030126",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/126",
abstract = "Structural enrichment is considered a useful tool to
improve the welfare conditions of captive fish by
deliberately increasing the physical heterogeneity and
complexity of captivity environments. However, the
potential effects of structural enrichment on the
stress response at the group level and on social
interactions have not been well studied yet. In this
study, we demonstrate that suspended vertical
structures (U-shaped ropes) can reduce behavioural
variability among fish groups (tank level) of European
seabass (Dicentrarchus labrax) juveniles. Differences
in behavioural responses during group risk-taking tests
(e.g., number of passes per fish) between treatments
were detected, and these responses in seabass in
enriched captive conditions were more homogeneous among
tanks compared to fish from non-enriched tanks. These
results suggest a positive effect of the structural
enrichment on social stabilisation and response to
stressful events at the tank level in seabass. However,
further research is still needed to improve the
knowledge of the potential effects of structural
enrichment on fish welfare and aquaculture management,
considering different enrichment designs, intensities,
and strategies according to farming conditions,
biological needs, and preferences of the fish species
and life-stage reared in captivity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lee:2022:ITA,
author = "Chorong Lee and Hye Jin Jeon and Bum Keun Kim and
Seong-Kyoon Choi and Sumi Kim and Gwang Il Jang and Ji
Hyung Kim and Jee Eun Han",
title = "Infectivity and Transmissibility of Acute
Hepatopancreatic Necrosis Disease Associated
\bioname{Vibrio parahaemolyticus} in Frozen Shrimp
Archived at $ - 80^\circ ${C}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "125",
day = "30",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030125",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/125",
abstract = "Acute hepatopancreatic necrosis disease (AHPND) caused
by Vibrio parahaemolyticus (Vp AHPND) has been reported
in commodity shrimp, but the potential risk of its
global spread via frozen shrimp in the shrimp trade is
yet to be fully explored. We hypothesized that frozen
shrimp with AHPND could be a source of Vp AHPND
transmission; thus, the infectivity of frozen shrimp
with AHPND was evaluated using a shrimp bioassay. To
prepare infected frozen shrimp, 12 Penaeus vannamei
(average weight, 2 g) were exposed to Vp AHPND by
immersion in water with a Vp AHPND concentration of
1.55 $ \times $ 10 7 CFU mL {$-$1}; once dead, the
shrimp were stored at {$-$80} {$^\circ $}C for further
analysis. After two weeks, a PCR assay was used to
confirm AHPND positivity in frozen shrimp (n = 2), and
Vp AHPND was reisolated from the hepatopancreases of
these shrimp. For the infectivity test, 10 P. vannamei
(average weight, 4 g) were fed with the
hepatopancreases of Vp AHPND -infected frozen shrimp (n
= 10). After feeding, 70\% of the shrimp died within
118 h, and the presence of Vp AHPND was confirmed using
a PCR assay and histopathology examination; moreover,
Vp AHPND was successfully reisolated from the
hepatopancreases of the dead shrimp. We are the first
to evaluate the potential transmissibility of Vp AHPND
in frozen shrimp, and our results suggest that frozen
shrimp with AHPND are a potential source of disease
spreading between countries during international
trade.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Su:2022:LTC,
author = "Li Su and Youwei Xu and Yongsong Qiu and Mingshuai Sun
and Kui Zhang and Zuozhi Chen",
title = "Long-Term Change of a Fish-Based Index of Biotic
Integrity for a Semi-Enclosed Bay in the \geoname{Beibu
Gulf}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "124",
day = "30",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030124",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/124",
abstract = "The Beibu Gulf in the northwestern South China Sea is
one of the world's most productive fishing grounds, yet
its biotic resources appear to be in rapid decline
because of overexploitation. Assessments of the health
status of the fisheries' resources in the gulf provide
a foundation for their conservation and management. As
fish accounted for 84\% to 97\% of the total catch in
the Beibu Gulf in the period 1962--2017, a Fish-based
Index of Biotic Integrity (F-IBI) was developed for the
Beibu Gulf, and data from otter trawl surveys during
the period 1962--2017 were used to measure variations
in the fish community. The assessment revealed a
generally downward trend in total fish catch density
(kg/km 2), catch density of 12 traditional commercial
demersal fish species, and percentage of
nektonic-feeding species, but an upward trend for the
percentage of fishes with a planktivorous or detritus
diet, percentage of pelagic species, and percentage of
the dominant species. The dominant species varied
greatly over the 50 year period and showed a tendency
towards small-sized species. The synthetic F-IBI
variable showed a downward trend and has indicated a
`fair' state since 1998. The decline in the F-IBI over
the last three decades suggests that anthropogenic
disturbances, especially overfishing, have had a
serious impact on the fish community of the Beibu Gulf.
The F-IBI is currently at risk of becoming `poor.'
Consequently, we suggest that rigid and enforceable
fishery management measures should be taken by both
China and Vietnam to prevent further deterioration of
the fisheries' resources in the gulf.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shrimpton:2022:FNT,
author = "J. Mark Shrimpton and Paige W. Breault and Luc A.
Turcotte",
title = "Fidelity to {Natal} Tributary Streams by {Kokanee}
Following Introduction to a Large Oligotrophic
Reservoir",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "123",
day = "30",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030123",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/123",
abstract = "The WAC Bennett Dam was completed in 1968 and
impounded the Upper Peace River to form the Williston
Reservoir in north central British Columbia. In 1990,
an enhancement project was initiated to stock Columbia
River Kokanee (non-anadromous Sockeye Salmon;
Oncorhynchus nerka) from southeastern British Columbia
into tributary streams that drained into regions of the
reservoir that were accessible by anglers. The current
distribution of spawning Columbia-origin Kokanee in the
Williston Reservoir watershed, however, does not
reflect the locations where these fish were initially
stocked and suggests extensive straying. Whether or not
Kokanee will develop fidelity to specific spawning
locations is not known, but it is important information
to effectively manage these introduced fish. We used
otolith microchemistry to estimate fidelity to natal
streams by Columbia-origin Kokanee in the Williston
Reservoir. Otolith elemental signatures for the region
of the otolith that formed during the larval period and
characterized the natal redd environment showed
considerable variation among samples. Natal signatures
tended to cluster for each river but not for all
spawners, suggesting elemental signatures from other
rivers. Homing to one of the four natal streams we
examined was classified to be 73\% based on linear
discriminant analysis, although variation in the
elemental signatures within each group suggests that
homing by Kokanee to specific natal streams may be as
low as 55\%. Based on similarity of water elemental
signatures for tributaries within large rivers,
however, the proportion of fish that returned to their
general region was likely higher and estimated to be
approximately 83\%. The result of regional homing could
be reproductive isolation and adaptation to local
conditions. It is unclear, however, if the current
estimated level of straying will limit genetic
differentiation and prevent local adaptation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Podhorec:2022:EHT,
author = "Peter Podhorec and Jind{\v{r}}i{\v{s}}ka Knowles and
Jakub Vyslou{\v{z}}il and Sergii Boryshpolets and
Anatolii Sotnikov and Martina Holick{\'a} and Jan
Kou{\v{r}}il and Borys Dzyuba",
title = "The Effect of Hormonal Treatment on Selected Sperm
Quality Parameters and Sex Steroids in Tropical
Cyprinid Bala Shark \bioname{Balantiocheilos
melanopterus}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "122",
day = "30",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030122",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/122",
abstract = "Hormone treatments are routinely employed with bala
shark Balantiocheilos melanopterus male broodstock to
stimulate the production of high-quality sperm. In the
current trial, three spermiation-inducing treatments
were evaluated: 20 {\mu}g/kg body weight (BW)
recombinant hCG; 20 {\mu}g/kg BW [D-Ala 6, Pro 9, NEt]-
gonadotropin-releasing hormone (GnRHa) with 20 mg/kg BW
metoclopramide; and poly(lactic-co-glycolic acid)
(PLGA) microparticles with slow release of 10 {\mu}g/kg
BW GnRHa. A 0.9\% saline AS negative control was also
included. Administration of the GnRHa through the form
of slow release of PLGA microparticles 24 h after
treatment resulted in a significantly higher sperm
volume, motility percentage, and total sperm count
compared to the control. Injection of GnRHa with
metoclopramide induced sperm parameters that did not
differ from the control, with the exception of motility
percentage. The lowest potency to induce spermiation in
bala sharks was in the treatment with recombinant hCG.
Both PLGA microparticles and GnRHa with metoclopramide
significantly increased blood plasma concentrations of
testosterone and 11-ketotestosterone compared to the
control. The PLGA microparticle system with continuous
release of 10 {\mu}g/kg BW GnRHa was the most effective
treatment in inducing spermiation in bala sharks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Politikos:2022:DVO,
author = "Dimitris V. Politikos and Nikolaos Sykiniotis and
Georgios Petasis and Pavlos Dedousis and Alba
Ordo{\~n}ez and Rune Vab{\o} and Aikaterini
Anastasopoulou and Endre Moen and Chryssi Mytilineou
and Arnt-B{\o}rre Salberg and Archontia Chatzispyrou
and Ketil Malde",
title = "\pkg{DeepOtolith v1.0}: an Open-Source {AI} Platform
for Automating Fish Age Reading from Otolith or Scale
Images",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "121",
day = "29",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030121",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib;
https://www.math.utah.edu/pub/tex/bib/gnu.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/121",
abstract = "Every year, marine scientists around the world read
thousands of otolith or scale images to determine the
age structure of commercial fish stocks. This knowledge
is important for fisheries and conservation management.
However, the age-reading procedure is time-consuming
and costly to perform due to the specialized expertise
and labor needed to identify annual growth zones in
otoliths. Effective automated systems are needed to
increase throughput and reduce cost. DeepOtolith is an
open-source artificial intelligence (AI) platform that
addresses this issue by providing a web system with a
simple interface that automatically estimates fish age
by combining otolith images with convolutional neural
networks (CNNs), a class of deep neural networks that
has been a dominant method in computer vision tasks.
Users can upload otolith image data for selective fish
species, and the platform returns age estimates. The
estimates of multiple images can be exported to conduct
conclusions or further age-related research.
DeepOtolith currently contains classifiers/regressors
for three fish species; however, more species will be
included as related work on ageing will be tested and
published soon. Herein, the architecture and
functionality of the platform are presented. Current
limitations and future directions are also discussed.
Overall, DeepOtolith should be considered as the first
step towards building a community of marine ecologists,
machine learning experts, and stakeholders that will
collaborate to support the conservation of fishery
resources.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{DIglio:2022:BIA,
author = "Claudio D'Iglio and Marco Albano and Sergio Famulari
and Nunziacarla Span{\`o} and Paola Rinelli and Serena
Savoca and Gioele Capillo",
title = "Basic Intersexuality (Abnormal Hermaphroditism) in the
Blackmouth Catshark, \bioname{Galeus melastomus},
({Rafinesque}, 1810), from the {Southern Tyrrhenian Sea
(Central Mediterranean Sea)}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "120",
day = "28",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030120",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/120",
abstract = "The present paper represents the first description of
abnormal hermaphroditism in Galeus melastomus
(Rafinesque, 1810). The black mouth cat shark specimen,
collected in summer from southern Tyrrhenian Sea at the
entrance of Salerno gulf, showed a basic intersexuality
characterized by the presence of male secondary and
female primary sexual characters. The reproductive
system has been described with an accurate
morphological analysis, also including a histological
assessment of oocytes. Results showed the presence of
only right clasper, not exceeding the pelvic fin in
length, with the absence of internal males' sexual
organs. Concerning the female primary characters, the
analysis of histological assessment showed the
structure of mature oocyte, confirming the maturity
stage of a mature female in active extruding stage, as
highlighted by the macroscopic morphological analysis.
Despite in many marine organisms' hermaphroditism is a
physiological condition showed by specimens during
their lifecycle, in Elasmobranchs this is considered an
abnormal condition, with some exceptions. The lack of
information on hermaphroditism in elasmobranchs form
the Mediterranean Sea requires an increase of
scientific community's attention, improving the
knowledge on the reproductive biology of this sensitive
taxon. This is essential to enhance the conservation of
elasmobranchs populations in the entire Mediterranean
basin.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xue:2022:IIC,
author = "Mingyang Xue and Zidong Xiao and Yiqun Li and Nan
Jiang and Wenzhi Liu and Yan Meng and Yuding Fan and
Lingbing Zeng and Yong Zhou",
title = "Isolation, Identification and Characteristics of
\bioname{Aeromonas caviae} from Diseased Largemouth
Bass (\bioname{Micropterus salmoides})",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "119",
day = "28",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030119",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/119",
abstract = "The largemouth bass (Micropterus salmoides) is one of
the most economically valuable fish species in China.
In this study, a bacterial pathogen was isolated from
the internal organs of diseased M. salmoides, and the
strain was named WH21406. This isolate was identified
as Aeromonas caviae on the basis of its morphology,
biochemical features and 16S rDNA phylogenetic
analysis. Four virulence genes related to
pathogenicity, namely, flagella (fla), elastase (ela),
haemolysin (hly) and aerolysin (aer), were detected in
this isolate. The median lethal dosage (LD50) of A.
caviae WH21406 for M. salmoides was calculated to be
3.46 $ \times $ 10 5 CFU mL {$-$1}. The
histopathological analysis showed obvious tissue damage
in the gill, liver, kidney, spleen and gut of the
diseased fish. The antibiotic susceptibility test
demonstrated that strain WH21406 was highly sensitive
to enrofloxacin, norfloxacin, streptomycin and
amikacin. The results of this study provide a
foundation for the diagnosis, prevention and treatment
of A. caviae infection in M. salmoides.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2022:FCM,
author = "Yingcen Li and Yunliang Miao and Xufang Liang and Shan
He",
title = "Functional Characterization and Molecular Marker
Development of the Proenkephalin as Biomarker of Food
Addiction in Food Habit Domestication of {Mandarin}
Fish (\bioname{Siniperca chuatsi})",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "118",
day = "27",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030118",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/118",
abstract = "Proenkephalin (PENK), as the precursor of endogenous
opioid enkephalin (ENK), is widely present in the
nervous system and plays an important role in animal
food addiction and rewarding behavior. In our study, we
intend to study the functional characterization and
molecular marker development of the penk gene related
to food habit domestication of mandarin fish. We found
that the penk gene of mandarin fish had three types of
endogenous opioid peptide sequences. Compared with
other tissues, penk mRNA was highly expressed in the
whole brain. Intracerebroventricular (ICV) injection of
lysine or methionine significantly increased the
expression of penk mRNA. The expression of penk mRNA in
the brain of mandarin fish that could be easily
domesticated from eating live prey fish to artificial
diets was significantly higher than those that could
not. After feeding with high-carbohydrate artificial
diets, the expression of penk mRNA showed no
significant difference between mandarin fish with
hypophagia and those that still ate normally. A total
of four single nucleotide polymorphisms (SNP) loci
related to easy domestication toward eating artificial
diets were screened from the mandarin fish population.
Additionally, the TT genotype at one of the loci was
significantly correlated with the food habit
domestication of mandarin fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vallecillos:2022:DFM,
author = "Antonio Vallecillos and Emilio Mar{\'\i}a-Dolores and
Javier Villa and Francisco Miguel Rueda and Jos{\'e}
Carrillo and Guillermo Ramis and Mohamed Soula and Juan
Manuel Afonso and Eva Armero",
title = "Development of the First Microsatellite Multiplex
{PCR} Panel for Meagre (\bioname{Argyrosomus regius}),
a Commercial Aquaculture Species",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "117",
day = "24",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030117",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/117",
abstract = "In this study, a microsatellite-based multiplex PCR
panel for meagre (Argyrosomus regius) was developed as
a useful and single tool in parental assignment and
population studies. Twenty-one specific and
interspecific microsatellites from different
aquaculture species of meagre (Argyrosomus regius),
Japanese meagre (A. japonicus), red drum (Sciaenops
ocellatus) and yellow meagre (Acoupa weakfish) were
assessed for genetic variability, allelic range and
genotype reliability. Finally, a SuperMultiplex for
Argyrosomus regius (SMAr) was designed with only the
best eight microsatellite markers. The panel assessment
was performed using a batch of brood stock from one
company and a sample of 616 offspring. It was possible
to assign 95\% of the offspring to a single pair of
parents using the exclusion method. It is therefore
considered an easy procedure, and a powerful and
low-cost tool for parental assignment to support
companies' breeding programs and to exchange
information between research groups.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ho:2022:CRO,
author = "Ching-Hsien Ho",
title = "Climate Risks and Opportunities of the Marine Fishery
Industry: a Case Study in \geoname{Taiwan}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "116",
day = "21",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030116",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/116",
abstract = "As climate change and extreme weather intensify,
forecasting natural environmental changes involves high
uncertainty and variation. Planned policy changes and
the ability of fisheries to withstand these changes and
impacts are major challenges in regard to addressing
future climate hazards in Taiwan. Taiwan's geographic
location is favourable for the marine fishery industry,
which is an important primary industry and a source of
animal protein for residents of Taiwan. Recently, both
long-term climate change and extreme climate hazards
have led to shocks in the marine fishery industry. The
resulting instability of the industry directly affects
the market supply of and demand for seafood. We conduct
a case study of a marine fishery industry in Taiwan
that incorporates social concern assessments of risk
perception and judgements of risk acceptance through
risk management processes. Furthermore, we incorporate
stakeholder participatory processes to reduce the gap
in awareness of adaptation and to increase the
opportunity to reach consensus and develop an
integrated risk management model that enables
decision-makers to better meet the needs of society.
These measures will enhance all parties' willingness to
adapt and help achieve the goal of public--private
collaboration in facing climate change challenges.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tang:2022:TSA,
author = "Xuemei Tang and Shulun Jiang and Henglin Wang and
Yanfeng Zhou and Fei Peng and Xizhao Zhang and Yifan
Zhou and Shiyue Guo and Yang You",
title = "Transcriptome Sequencing Analysis Reveals Dynamic
Changes in Major Biological Functions during the Early
Development of Clearhead Icefish, \bioname{Protosalanx
chinensis}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "115",
day = "21",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030115",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/115",
abstract = "Early development, when many important developmental
events occur, is a critical period for fish. However,
research on the early development of clearhead icefish
is very limited, especially in molecular research. In
this study, we aimed to explore the dynamic changes in
the biological functions of five key periods in
clearhead icefish early development, namely the YL
(embryonic), PM (first day after hatching), KK (fourth
day after hatching), LC (seventh day after hatching),
and SL (tenth day after hatching) stages, through
transcriptome sequencing and different analysis
strategies. A trend expression analysis and an
enrichment analysis revealed that the expression
ofgenes encoding G protein-coupled receptors and their
ligands, i.e., prss1\_2\_3, pomc, npy, npb, sst, rln3,
crh, gh, and prl that are associated with digestion and
feeding regulation gradually increased during early
development. In addition, a weighted gene co-expression
network analysis (WGCNA) showed that eleven modules
were significantly associated with early development,
among which nine modules were significantly positively
correlated. Through the enrichment analysis and hub
gene identification results of these nine modules, it
was found that the pathways related to eye, bone, and
heart development were significantly enriched in the YL
stage, and the ccnd2, seh1l, kdm6a, arf4, and ankrd28
genes that are associated with cell proliferation and
differentiation played important roles in these
developmental processes; the pak3, dlx3, dgat2, and
tas1r1 genes that are associated with jaw and tooth
development, TG (triacylglycerol) synthesis, and umami
amino acid receptors were identified as hub genes for
the PM stage; the pathways associated with aerobic
metabolism and unsaturated fatty acid synthesis were
significantly enriched in the KK stage, with the foxk,
slc13a2\_3\_5, ndufa5, and lsc2 genes playing important
roles; the pathways related to visual perception were
significantly enriched in the LC stage; and the bile
acid biosynthetic and serine-type peptidase activity
pathways were significantly enriched in the SL stage.
These results provide a more detailed understanding of
the processes of early development of clearhead
icefish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2022:FVC,
author = "Tsung-Yu Yang and Hsing-Chun Lin and Wen-Hong Liu",
title = "The Fishery Value Chain Analysis in \geoname{Taiwan}",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "114",
day = "16",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030114",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/114",
abstract = "Although the GDP of the fishery sector accounts for
less than 0.3\% of Taiwan's GDP, there are many
secondary and tertiary industries related to fisheries
that add great value to the country's economy.
Therefore, when analyzing and calculating the output
value and value added of the fishery sector, the
relevance of these upstream and downstream industries
should be considered jointly, and the value chain of
the fishery industry should be studied and analyzed in
order to truly present the value added created by
fishery. This study aims to do this by first analyzing
not only the primary fishery industry, but the value
chain of the fishery industry at different stages such
as fishery production, marine fishing, aquaculture,
fishery processing, retail and services, and supportive
systems. Then the value added of the whole fishery
value chain to Taiwan's GDP is estimated by using the
Leontief input--output model, from the perspective of
industry linkages and covering the period from 2011 to
2018. The empirical results suggest that the value
added created by fishery and related industries
accounted for 2.96\% of the GDP in 2011, which rose to
3.11\% in 2014, fell to 2.87\% in 2015, rose to 2.93\%
in 2017, and fell to 2.84\% in 2018. From 2011 to 2018,
the amount of value added created by fisheries and
related industries rose from NT$ 422.8 b i l l i o n t
o N T $ 520.9 billion. The primary fishery sector has
the largest contribution, followed by the fishery input
sector, and transportation and sale service sector, and
the fishery product processing sector has the lowest
contribution rate. The empirical results provide
important implications and references to the policy
makers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nguyen:2022:UFP,
author = "Thi Hanh Nguyen and San-Lang Wang and Thi Huyen Nguyen
and Manh Dung Doan and Thi Ha Trang Tran and Van Anh
Ngo and Nhat Duoc Ho and Thi Ngoc Tran and Chien Thang
Doan and Van Chung Do and Anh Dzung Nguyen and Van Bon
Nguyen",
title = "Utilization of Fishery-Processing By-Product Squid
Pens for Scale-Up Production of Phenazines via
Microbial Conversion and Its Novel Potential
Antinematode Effect",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "113",
day = "15",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030113",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/113",
abstract = "Fishery by-products (FBPs) have been increasingly
investigated for the extraction and production of a
vast array of active molecules. The aim of this study
was to produce phenazine compounds from FBPs via
microbial fermentation and assess their novel
antinematode effect. Among various FBPs, squid pen
powder (SPP) was discovered as the most suitable
substrate for phenazine production by Pseudomonas
aeruginosa TUN03 fermentation. Various small-scale
experiments conducted in flasks for phenazine
production indicated that the most suitable was the
newly designed liquid medium which included 1\% SPP,
0.05\% MgSO 4, and 0.1\% Ca 3 (PO 4) 2 (initial pH 7).
Phenazines were further studied for scale-up
bioproduction in a 14 L bioreactor system resulting in
a high yield (22.73 {\SGMLmicro}g/mL) in a much shorter
cultivation time (12 h). In the fermented culture
broth, hemi-pyocyanin (HPC) was detected as a major
phenazine compound with an area percentage of 11.28\%
in the crude sample. In the bioactivity tests, crude
phenazines and HPC demonstrate novel potential
nematicidal activity against black pepper nematodes,
inhibiting both juveniles (J2) nematodes and egg
hatching. The results of this work suggest a novel use
of SPP for cost-effective bioproduction of HPC, a novel
potential nematodes inhibitor. Moreover, the
combination of MgSO 4 and Ca 3 (PO 4) 2 was also found
to be a novel salt composition that significantly
enhanced phenazine yield by P. aeruginosa fermentation
in this work.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhu:2022:IOD,
author = "Yahua Zhu and Tao Jiang and Xiubao Chen and Hongbo Liu
and Quinton Phelps and Jian Yang",
title = "Inter-Otolith Differences in Strontium Markings: a
Case Study on the Juvenile Crucian Carp
\bioname{Carassius carassius} ({Linnaeus}, 1758)",
journal = j-FISHES,
volume = "7",
number = "3",
pages = "112",
day = "15",
month = may,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7030112",
ISSN = "2410-3888",
bibdate = "Wed Jul 6 07:29:57 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/3/112",
abstract = "The release of hatchery-reared fish fry for restocking
is important for the enrichment of fishery resources;
however, the effective evaluation of the success rate
of marking such fish is challenging. We exposed
juvenile crucian carp (Carassius carassius) to a single
concentration of SrCl 2 {\cdot}6H 2 O for 5 d and
evaluated the efficiency of Sr marking of the fish
otoliths (sagittae, asterisci, and lapilli) using an
electron probe micro-analyzer. Sr marking signatures
formed a peak in all otolith types, with a marking
success rate of 100\%. The ratio of Sr to Ca in the
lapilli and sagittae was higher than that in the
asterisci. It took 2 d from the beginning of immersion
to the deposition of Sr on the lapilli and sagittae,
and the time delay for asterisci was 1 d. For the
lapilli and sagittae, it took 16 d to terminate Sr
marking and fully recover to the pre-marking Sr level,
whereas it was 12 d for the asterisci. The application
of the Sr dose had no effect on the survival or growth
of the carp. This study demonstrated that the lapilli
are the most suitable otolith type for Sr marking
observations in crucian carp and provides a theoretical
basis and technical support for carp restocking using
the Sr marking approach.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Guimaraes:2022:OAP,
author = "Mateus Cardoso Guimar{\~a}es and Isabel M. Cerezo and
Miguel Frederico Fernandez-Alarcon and Mariene Miyoko
Natori and Luciana Yuri Sato and Camila A. T. Kato and
Miguel Angel Mori{\~n}igo and Silvana Tapia-Paniagua
and Danielle de Carla Dias and Carlos Massatoshi
Ishikawa and Maria Jos{\'e} T. Ranzani-Paiva and Luara
Lucena Cassiano and Erna Elisabeth Bach and
Patr{\'\i}cia B. Clissa and Daniele P. Orefice and
Leonardo Tachibana",
title = "Oral Administration of Probiotics (\bioname{Bacillus
subtilis} and \bioname{Lactobacillus plantarum}) in
\geoname{Nile} Tilapia (\bioname{Oreochromis
niloticus}) Vaccinated and Challenged with
\bioname{Streptococcus agalactiae}",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "211",
day = "22",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040211",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/211",
abstract = "Streptococcus agalactiae is an important bacterial
pathogen in intensive Nile tilapia production, causing
high mortality rates and great economic losses. This
work aimed to evaluate the Nile tilapia vaccination
against S. agalactiae and fed with ration containing
probiotic AQUA PHOTO {\reg} composed of Bacillus
subtilis and Lactobacillus plantarum, on the immune
response action and gut microbiota. The experimental
design was completely randomized with five treatments
(CON = control; ADJ = adjuvant; PRO = probiotic; VAC =
vaccine; PRO + VAC = probiotic + vaccine) and five
replicates. The vaccine (bacterin + adjuvant) was
injected after 21 days (21d) of probiotic feeding and
the vaccine was booster 14 days post-vaccination (35d).
After 14 days of the booster (49d), the fish were
challenged with S. agalactiae and observed for more
than 14 days, completing 63 days. The immunized group
showed a better survival rate (CON 40\%; ADJ 57\%; PRO
67\%; VAC 87\%; PRO + VAC 97\%). The treatments VAC and
PRO + VAC, after booster produced higher levels of IgM
antibodies compared with the control from the same
time. The combination of probiotic and vaccination
provided better protection against S. agalactiae
infection, directly affecting the gut microbiological
profile. These results indicated the contribution of
probiotic to the adaptive immune response through the
modulation of the intestinal microbiota, improving the
effect of the vaccination. In conclusion, AQUA PHOTO
{\reg}, composed of B. subtilis and L. plantarum,
orally administered to Nile tilapia vaccinated against
and challenged with S. agalactiae increases protection
from infection and modifies the intestinal microbiota
profile of the host, promoting the microbiota balance
and improving adaptive immune response.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lokovsek:2022:IFE,
author = "Ana Lokov{\v{s}}ek and Martina Orlando-Bonaca and
Domen Trkov and Lovrenc Lipej",
title = "An Insight into the Feeding Ecology of
\bioname{Serranus scriba}, a Shallow Water Mesopredator
in the \geoname{Northern Adriatic Sea}, with a
Non-Destructive Method",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "210",
day = "20",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040210",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/210",
abstract = "Serranus scriba is a common member of the coastal fish
community in the Adriatic Sea, but knowledge about its
feeding ecology is scarce. The aim of this paper is to
present new evidence about its food preferences and
feeding habits. An innovative non-destructive method of
fecal pellet analysis was used for this study. This
method does not require sacrificing specimens and the
fish can be released back into the sea alive after the
laboratory work. The results demonstrated that S.
scriba mainly preys on decapods, followed by
polychaetes, isopods, fish, mollusks and swarming
shrimps. The calculated index of trophic diversity
(ITD) value of 0.89 indicates that it is an
opportunistic feeder that feeds on a wide range of
different prey. According to the calculated trophic
level of 3.43, which is higher than that of other
members of the community, S. scriba is also an
important piscivorous predator. With age, S. scriba
undergoes an ontogenetic shift. The proportion of
crustaceans, gastropods and polychaetes decreases with
age and body size, while the proportion of fish
increases.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garcia-Marquez:2022:FEA,
author = "Jorge Garc{\'\i}a-M{\'a}rquez and Isabel M. Cerezo and
F{\'e}lix L. Figueroa and Roberto Te{\'o}filo
Abdala-D{\'\i}az and Salvador Arijo",
title = "First Evaluation of Associated Gut Microbiota in Wild
Thick-Lipped Grey Mullets (\bioname{Chelon labrosus},
{Risso} 1827)",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "209",
day = "19",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040209",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/209",
abstract = "This study aimed to characterize the intestinal
microbiota of wild thick-lipped grey mullets (Chelon
labrosus) and explore its potential functionality on
the host. Intestinal contents of anterior and posterior
sections from wild fish were collected and DNA was
extracted. Subsequently, the V3--V4 regions of 16S rRNA
were sequenced using the Illumina technology and
results were analyzed by bioinformatics pipeline. The
functional profile of the microbial community was
analyzed using PICRUSt software. Shannon and Simpson
diversity indices were significantly higher in the
posterior section of wild specimens. The overall
taxonomic composition suggests a certain homogeneity in
the anterior section of the intestine and heterogeneity
in the posterior section. Due to this, no statistical
differences were detected at any level among both
intestinal sections. Predicted functions of intestinal
microbiota showed the most abundant were those related
to amino acid metabolism, carbohydrate metabolism,
energy metabolism, membrane transport, and cell
replication and repair. Furthermore, the analysis
revealed microbial functional genes related to the
elimination of environmental toxins.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garcia-Fernandez:2022:UDG,
author = "Cristina Garc{\'\i}a-Fern{\'a}ndez and Rosario
Dom{\'\i}nguez-Petit and Fran Saborido-Rey",
title = "The Use of Daily Growth to Analyze Individual Spawning
Dynamics in an Asynchronous Population: The Case of the
\geoname{European} Hake from the Southern Stock",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "208",
day = "18",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040208",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/208",
abstract = "Daily growth patterns and their relationship with
reproduction was analyzed in the European hake from the
Galician Shelf, where it shows a very protracted
spawning with three spawning peaks. The daily growth
analysis was performed in otoliths of adult females on
the transversal section of the sagittae otolith. Daily
increments were measured from the border to the nucleus
in females until they were discernible. Results show
that daily growth of females decreases during the
spawning period because they allocate less energy to
somatic growth in favor of the production of gametes,
with an increase in growth in July. Lastly, daily
growth individual trends showed a ``spawning pattern''
in 28\% of medium and large females, suggesting an
individual spawning period of one to two months, with
4--5 valleys of narrow daily increments, likely
associated to batch release: individual spawning
frequency would be 4--5 days. This is the first time
that individual spawning frequency in hake is estimated
based on individual data. Finally, the spawning pattern
is detected only once per year, indicating that a
single female participates only in one spawning peak
per year, supporting the hypothesis of the existence of
two or more spawning components in the stock.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pang:2022:CUL,
author = "Yangyang Pang and Chao Niu and Lifeng Wu and Yameng
Song and Xiaozhe Song and Ao-ya Shi and Xingliang Shi
and Zong-wen Wu and Boping Tang and Xiaozhen Yang and
Yongxu Cheng",
title = "Comprehensive Utilization of Land Resources for
Photovoltaic Power Generation to Culture
\geoname{Chinese} Mitten Crab (\bioname{Eriocheir
sinensis}): Growth Performance, Nutritional Composition
and Tissue Color",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "207",
day = "18",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040207",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/207",
abstract = "The assertive growth of photovoltaics (PV) will occupy
a lot of land resources. There is also a needed land
resource to expand the culturing area of Eriocheir
sinensis. The aquavoltaic systems offer a potential
solution to integrate PV power and E. sinensis
culturing. In this study, we cultured E. sinensis in an
area of PV panels (PV group) and an area with no PV
panels (control group), respectively. The results
showed that the weight gain rate, body length, body
width, and meat yield of male crabs in the PV group
significantly increases. In addition, the moisture of
muscles, hepatopancreas, and testes in the PV group has
significantly increased, and the total lipids of the
hepatopancreas and muscles in the PV group were
significantly decreased. Moreover, the PV panels
affected the content of eight amino acids in different
tissues, including Met, Arg, Cys, Pro, Gly, Leu, Tyr,
and His. In addition, several saturated fatty acids
(SFA), monounsaturated fatty acids (MUFA), and
polyunsaturated fatty acids (PUFA) had been
significantly influenced by the PV panels.
{\SGMLsum}MUFA, docosahexaenoic acid (DHA),
{\SGMLsum}n-3 PUFA, {\SGMLsum}n-6 PUFA, and n-3/n-6
PUFA ratio ($ p < 0.05$) in the PV group had
significantly decreased. The results of tissue color
showed that the L* value of ovaries was significantly
higher than in the control group, and a* and b* values
of hepatopancreas were significantly lower. In
conclusion, PV panels could promote growth performance
and amino acid nutrition of crabs. However, the PV
panels had a few negative influences on the fatty acid
composition and tissue color.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2022:EAA,
author = "Mingshuai Li and Shihui Wang and Zhigang Zhao and
Liang Luo and Rui Zhang and Kun Guo and Lanlan Zhang
and Yuhong Yang",
title = "Effects of Alkalinity on the Antioxidant Capacity,
Nonspecific Immune Response and Tissue Structure of
\geoname{Chinese} Mitten Crab \bioname{Eriocheir
sinensis}",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "206",
day = "18",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040206",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/206",
abstract = "The effects of various levels of alkalinity stress (0,
18.25, 35.41, 52.53 and 69.74 mmol/L) in Chinese mitten
crab (Eriocheir sinensis) were investigated by means of
measuring hepatopancreas antioxidant system and serum
nonspecific immune system-related indices at 0, 12, 24,
48 and 96 h, hepatopancreas tissue structure at 96 h.
The activities of superoxide dismutase (SOD), catalase
(CAT), glutathione (GSH) and aspartate aminotransferase
(AST) in each concentration group generally showed a
trend of a first increasing and then decreasing during
the 96-h stress process ($ p < 0.05$)1, while no
significant changes were observed in the blank group
during this period ($ p > 0.05$). The activities of
CAT, GSH and AST in all treatment reached peak values
at 24--48 h. At 96 h of alkalinity stress, the
activities of GSH, alanine aminotransferase (ALT), acid
phosphatise (ACP) and alkaline phosphatise (AKP) in the
18.25 mmol/L group were not significantly different
with the control group ($ p < 0.05$). The activities of
SOD and CAT in the 52.53 and 69.74 mmol/L treatment
were significantly lower than those in the control
group ($ p < 0.05$), and the level of total antioxidant
capacity(T-AOC), ALT, ACP and AKP in the 69.74 mmol/L
group were significantly higher than those in the other
groups ($ p < 0.05$). Hepatopancreatic histological
observation showed that the hepatopancreas of E.
sinensis in the control group was normal. With
increasing alkalinity, the basal membrane of the
hepatopancreas fell off or even ruptured. Additionally,
the number of hepatopancreas vacuoles increased, the
volume of B cells and their internal transport vesicles
increased, epithelial cells disintegrated, and the
nucleus gradually shrank. E. sinensis can activate
antioxidant and nonspecific immune systems to adapt to
alkalinity stress. However, oxidative stress, immune
system damage and hepatopancreas structure damage were
caused when the stress exceeded the adaptive capacity
of the body.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mahu:2022:CRA,
author = "Edem Mahu and Salieu Sanko and Allieubakarr Kamara and
Ernest Obeng Chuku and Elizabeth Effah and Zacharie
Sohou and Yaovi Zounon and Victoria Akinjogunla and
Ruth Oluwatoyin Akinnigbagbe and Hamet Diaw Diadhiou
and Robert Marchant",
title = "Climate Resilience and Adaptation in \geoname{West
African} Oyster Fisheries: An Expert-Based Assessment
of the Vulnerability of the Oyster \bioname{Crassostrea
tulipa} to Climate Change",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "205",
day = "16",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040205",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/205",
abstract = "Globally, over 85\% of oyster reefs have been lost,
and the combined effects of climate change, ocean
acidification, and environmental degradation, including
pollution and mangrove overharvesting, could further
reduce global oyster fisheries in the coming decades.
To understand the level of impact of climate change on
the oyster fishery in West Africa, an expert-based
vulnerability assessment to climate change was
conducted for the West African mangrove oyster
(Crassostrea tulipa, Lamarck 1819). Using a combination
of the exposure of the oyster to climatic stressors
(estuarine temperature, salinity, river flow, surface
run-off, sea level rise, and estuarine circulation)
together with an assessment of sensitivity to these
stressors, we estimate the overall vulnerability of C.
tulipa to climate change. A very high overall climate
vulnerability score of 12 on a scale of 16 was
calculated for C. tulipa. While the overall climate
exposure score in the West African coastal region
remained high, the high sensitivity of C. tulipa to
hydrographic conditions of its habitat, in particular
salinity, coupled with its sessile and habitat-specific
nature, pushed the overall vulnerability to very high.
Early life history settlement requirements, adult
mobility, and sensitivity to salinity were the three
most important biological and sensitivity attributes
that determined the vulnerability score. By leaving
each of these three sensitivity attributes out of the
analysis, the overall vulnerability score was reduced
to 9 (i.e., from very high to high). A negative
directional effect of climate change, coupled with a
low potential for change in distribution, threatens the
C. tulipa fishery in a long-term adverse climate
scenario. We recommend management efforts that
incorporate climate resilience and adaptation practices
to prioritize recruitment success, as well as the
development of breeding lines with climate-resilient
traits.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Armas:2022:IFP,
author = "Elier Armas and Hugo Arancibia and Sergio Neira",
title = "Identification and Forecast of Potential Fishing
Grounds for Anchovy (\bioname{Engraulis ringens}) in
{Northern Chile} Using Neural Networks Modeling",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "204",
day = "15",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040204",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/204",
abstract = "Engraulis ringens (E. ringens) is a small pelagic fish
of which the geographic and bathymetric distribution is
conditioned by fluctuations in oceanographic conditions
at different time scales (daily, weekly, monthly,
annually, supra-annually, and longer) and by fishing.
Understanding the {organism$-$ environment}
interactions and predicting the spatial distribution of
its schools can improve conservation actions and
fishery management, along with the operation of the
fleets targeting E. ringens. There is an important
fishery of E. ringens in Northern Chile ({18$^\circ
$}21{\prime} S--{26$^\circ $}00{\prime} S), which
provides about 80\% of the purse seine catch. To
identify and predict potential fishing grounds for E.
ringens in this system, we implemented a predictive
model of fishing grounds based on neural networks,
which was trained with the georeferenced data of daily
catches by industrial purse sein ships from 2003 to
2020 and information on oceanographic variables (sea
surface temperature, salinity, depth of the mixed
layer, sea height, and currents) obtained from the
Copernicus Marine Enviroment Monitoring Service (CMEMS
program). The neural network model had a very good
performance (86\%). Longitude (23\%) was the most
relevant variable for identifying potential fishing
grounds, followed by the mixed layer depth (18\%),
latitude (15\%), sea surface temperature (12\%), month
(12\%), sea height (9\%), salinity (9\%), and the zonal
and meridional components of the current velocity
(2\%). The neural network model classified correctly
the majority of the areas with and without fishing
potential; thus, its use is recommended to predict
fishing grounds for E. ringens in the study area. Its
application could increase by 88\% of the probability
of capture anchovy by the purse seine fleet of Northern
Chile.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2022:EDC,
author = "Mingliang Zhang and Xinyi Wu and Shaowei Zhai",
title = "Effect of Dietary Compound Acidifiers Supplementation
on Growth Performance, Serum Biochemical Parameters,
and Body Composition of Juvenile \geoname{American} Eel
(\bioname{Anguilla rostrata})",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "203",
day = "12",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040203",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/203",
abstract = "As growth-promoting feed additives, compound
acidifiers (CAs) have been widely reported in many
farmed fish species, whereas there is little
information on the application of CAs in the eel diet.
The present trial was conducted to evaluate the
supplementation effects of CAs on growth performance,
serum biochemical parameters, and body composition of
the juvenile American eel (Anguilla rostrata). The CAs
supplementation levels in the diet of American eel were
0, 2.0, 3.0, 4.0, and 5.0 g/kg, respectively. The trial
lasted for 12 weeks. The most beneficial effects of CAs
on promoting growth performance, decreasing levels of
serum lipid, modifying parameters of the liver function
and immune function in the serum, and increasing the
utilization of calcium and phosphorus in the diet were
observed in the fish fed with 4.0 g/kg CAs. Dietary CAs
supplementation had no significant effects on the body
composition of whole fish, whereas 5.0 g/kg of dietary
CAs supplementation exerted negative effects on growth
performance, serum biochemical parameters, and
utilization of calcium and phosphorus. The recommended
level of CAs in the diet was 3.5--3.7 g/kg to achieve
the optimal weight gain and feed conversion ratio for
the juvenile American eel.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huang:2022:DPM,
author = "Dongyu Huang and Hualiang Liang and Jian Zhu and
Mingchun Ren and Xianping Ge",
title = "Dietary Protein Modifies Hepatic Glycolipid
Metabolism, Intestinal Immune Response, and Resistance
to \bioname{Streptococcus agalactiae} of Genetically
Improved Farmed Tilapia ({GIFT}: \bioname{Oreochromis
niloticus}) Exposed to High Temperature",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "202",
day = "12",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040202",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/202",
abstract = "The present study investigates the effects of dietary
protein levels on glucolipid metabolism, immune
function, and resistance to Streptococcus agalactiae of
genetically improved farmed tilapia (GIFT) exposed to
high temperature. Six practical diets were prepared to
feed 360 fish (initial weight 43.78 {\pm} 0.12 g) with
graded protein levels (26.45\%, 29.28\%, 31.69\%,
33.68\%, 36.18\%, and 38.75\% dry matter). The results
showed that 26.45\% dietary protein significantly
improved glycolysis by increasing PK mRNA levels, while
the 29.28\% and 31.69\% dietary protein levels promoted
gluconeogenesis by increasing PEPCK and G6Pase mRNA
levels. For lipid metabolism, 26.45\% dietary protein
enhanced lipid synthesis by increasing PPAR-{\gamma},
SREBP1c, and FAS mRNA levels, while 31.69\% dietary
protein enhanced the level of lipolysis by increasing
the PPAR-{\alpha} and CPT1 mRNA levels. The highest
plasma TG and TC contents were observed in the 29.28\%
and 31.69\% dietary protein groups, respectively. In
terms of antioxidants and immunity, the 31.69\% dietary
protein level activated the expression levels of HSP90
mRNA, thus increasing the expression levels of
antioxidant-related genes (CAT, SOD, and GPx), and
upregulating the anti-inflammatory factor IL-10 mRNA
levels. In addition, regarding the antioxidant enzymes,
the highest GSH content was found in the 29.28\%
dietary protein group, while the 31.69\% dietary
protein group had the maximum GSH-Px activity. The
lowest plasma ALT and AST activities were observed in
the 31.69\% dietary protein group. Ultimately, the
survival rate of juvenile GIFT fed 31.69\% dietary
protein was highest after a Streptococcus agalactiae
challenge. Overall, 29.28--31.69\% dietary protein was
recommended in the diet of GIFT in a high-temperature
environment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Iqbal:2022:IDF,
author = "Usama Iqbal and Daoliang Li and Muhammad Akhter",
title = "Intelligent Diagnosis of Fish Behavior Using Deep
Learning Method",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "201",
day = "11",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040201",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/201",
abstract = "Scientific methods are used to monitor fish growth and
behavior and reduce the loss caused by stress and other
circumstances. Conventional techniques are
time-consuming, labor-intensive, and prone to
accidents. Deep learning (DL) technology is rapidly
gaining popularity in various fields, including
aquaculture. Moving towards smart fish farming
necessitates the precise and accurate identification of
fish biodiversity. Observing fish behavior in real time
is imperative to make better feeding decisions. The
proposed study consists of an efficient end-to-end
convolutional neural network (CNN) classifying fish
behavior into the normal and starvation categories. The
performance of the CNN is evaluated by varying the
number of fully connected (FC) layers with or without
applying max-pooling operation. The accuracy of the
detection algorithm is increased by 10\% by
incorporating three FC layers and max pooling
operation. The results demonstrated that the shallow
architecture of the CNN model, which employs a
max-pooling function with more FC layers, exhibits
promising performance and achieves 98\% accuracy. The
presented system is a novel step in laying the
foundation for an automated behavior identification
system in modern fish farming.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sung:2022:PTF,
author = "Wei-Ying Sung and Hsiao-Chien Lee and Wen-Hong Liu",
title = "The Path from Traditional Fisheries to Ecotourism in
\geoname{Cimei Island}",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "200",
day = "10",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040200",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/200",
abstract = "Cimei Island is a second-class outlying island. In
recent years, due to the lack of coastal fishery
resources and restrictions on traffic and climate, the
traditional fishery and tourism industries that
residents rely on for a living have faced challenges.
This research is based on the Barbados Programme of
Action, from the perspective of environmental
conservation, industrial economy, and social
development in sustainable development, and from the
perspective of local stakeholders, to construct a
sustainable tourism action approach and development
mechanism in Cimei Island. A qualitative research
method was adopted. Various sources of data, including
focus discussions and in-depth interviews with local
stakeholders, textual materials, and field
observations, were collected and analyzed. The results
are as follows: (1) Cimei faces great threats in
social, economic, and environmental aspects, which has
led to Cimei Islands' promotion of permanent
development. (2) The current development of Cimei
cannot effectively drive industrial development or the
transformation of fishing villages. However, residents
are worried that overdevelopment will impact the
ecological environment and lifestyle on the Islands.
(3) Cimei's sustainable tourism development approach
should be to first take inventory of the ecological
environment and cultural resources, plan related
environmental laws and regulations, and finally, use
sustainable tourism to drive industrial development to
carry out island development.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Giovos:2022:UGN,
author = "Ioannis Giovos and Roxani Naasan Aga-Spyridopoulou and
Fabrizio Serena and Alen Soldo and Adi Barash and
Nikolaos Doumpas and Georgios A. Gkafas and Dimitra
Katsada and George Katselis and Periklis Kleitou and
Vasileios Minasidis and Yannis P. Papastamatiou and
Eleana Touloupaki and Dimitrios K. Moutopoulos",
title = "An Updated \geoname{Greek} National Checklist of
Chondrichthyans",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "199",
day = "09",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040199",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/199",
abstract = "Accurate checklists of species are essential for
evaluating their conservation status and for
understanding more about their distribution, biology
and ecology and, therefore, the first step in order to
effectively protect them. According to the existing
literature, the Greek seas are rich in chondrichthyan
biodiversity and herein, we update the most recent
chondrichthyan checklist for the country regarding the
species that are present in the Greek waters, correct
unvalidated miscellaneous sightings and observations
and provide guidelines about future research to improve
their conservation. In total, 330 sources were
collected from which 276 were used for further
analysis, resulting in 1485 records of 67 species,
among which 61 are confirmed by experts, including 34
sharks, 26 batoids and one chimaera. We are further
listing six species as ``Questionable/Not
Confirmed''.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2022:DMP,
author = "Bin Wang and Hanping Mao and Jian Zhao and Yong Liu
and Yafei Wang and Xiaoxue Du",
title = "Designing a Multi-Parameter Method to Assess the
Adaptation Period of Crucian Carp under Stress
Conditions of the Bionic Robot Fish",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "198",
day = "08",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040198",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/198",
abstract = "Changes in the physiological and behavioral states of
fish are affected by foreign substances. Therefore,
fish need a certain adaptation period to eliminate the
stress response. Herein, in order to determine the
adaptation period, the bionic robot fish was used to
obtain behavioral information about crucian carp, which
was tested at five time points (1st, 7th, 14th, 21st
and 28th day) within 28 days. First, the fear response
and exploratory behavior of crucian carp affected by
three-color bionic robot fish were explored. Then,
according to the measurement results of the behavior,
morphology, and feeding, and the physiological and
biochemical properties of the crucian carp, a
multi-parameter evaluation method was proposed to
determine the adaptation period of the crucian carp
under this monitoring mode. The results showed that
more than 4 areas were occupied by crucian carp from
the 21st day. From the 16th day, the number of crucian
carp swimming with clear outlines gradually increased.
The number of abnormal swimming occurrences decreased
on day 13. More than 80\% of the crucian carp body
color returned to dark on the 23rd day. The crucian
carp did not respond to food until day 19, when most of
the crucian carp began to scramble for food. Food
consumption reached more than two thirds on day 22. In
addition, glucose and total protein leveled off after
day 21, when mean hemoglobin levels were highest.
Triglycerides showed a trend of first decreasing and
then increasing. The pigmented area of the skin section
gradually decreases and eventually stabilizes. In
summary, it takes at least 23 days for the crucian carp
to adapt to the influence of the bionic robot fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Axling:2022:BZL,
author = "Johanna Axling and Hampus Jakobsson and Natalia Frymus
and Per-Ove Th{\"o}rnqvist and Erik Petersson and
Svante Winberg",
title = "Boldness in Zebrafish Larvae---Development and
Differences between a Domesticated Lab Strain and
Offspring of Wild-Caught Fish",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "197",
day = "08",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040197",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/197",
abstract = "Zebrafish (Danio rerio) are becoming one of the most
important model organisms in behavioural neuroscience.
It has been shown repeatedly that different zebrafish
strains show large behavioural differences. These
divergent behavioural profiles may have a genetic
basis, but environmental factors and previous
experience are also known to greatly affect the
behavioural phenotype of zebrafish. It could be
expected that behavioural differences at the larval
stage should be less affected by environmental factors
and experience. In the present study, we screened
larvae of zebrafish of the AB strain and offspring of
wild-caught zebrafish for boldness, using an open field
test. In order to follow the behavioural development,
we studied larvae at the age of 5-, 7-, 12- and 30-days
post fertilization (dpf). Behaviour, as well as
behavioural development, clearly differed between the
larvae of the different strains. Wild larvae showed
larger total distance moved than AB larvae, both at
light and dark conditions. These differences were
already present at 12 dpf but became more pronounced
with age. Wild larvae had a greater variance compared
to AB larvae for most of the variables. We have
previously shown that bold and shy adult zebrafish
differ in the brain expression of dopamine and opioid
receptors. The results of the current study show that
wild larvae display significantly higher brain
expression of drd2b than AB larvae at 30 dpf, a
difference that could be related to differences in
activity. We did not detect any differences in the
expression of opioid receptors.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2022:MCN,
author = "Xiaomei Zhou and Dafang Zhao and Yuan Chen and Yangbo
Xiao and Zhuangwen Mao and Shenping Cao and Fufa Qu and
Yutong Li and Junyan Jin and Zhen Liu and Jianzhong Li
and Zhimin He",
title = "Molecular Characterization and Nutrition Regulation of
the Glutamine Synthetase Gene in Triploid Crucian
Carp",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "196",
day = "08",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040196",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/196",
abstract = "Glutamine synthetase (GS) is a key enzyme that
catalyzes the synthesis of glutamine from glutamate,
which plays a role in the promotion of muscle cell
growth and in improving the flavor of meats. In this
study, a GS gene encoding 371 amino acids was cloned
from triploid crucian carp and showed the highest level
of similarity with the GS gene found in Cyprinus
carpio. Meanwhile, GS was differentially expressed in
different tissues, and its day--night expression
changes showed obvious oscillation. Additionally, the
effects of glutamate and glutamine on GS expression in
muscle cells were investigated in vitro and in vivo. We
found that its expression was obviously increased due
to high levels of glutamate (2 mg/mL) but decreased by
glutamine in vitro. However, it was significantly
promoted by glutamate and glutamine in vivo, with an
optimal concentration of 2\%. Furthermore, the use of
lysine--glutamate dipeptides as feed additives also had
a positive influence on GS expression (the optimal
concentration is 0.8\%). Finally, we explored the
effects of different protein levels and sources on the
expression of GS, and the results demonstrated that GS
had the highest expression at the 35\% protein level,
but no significant differences were observed in the
different protein sources between the fish meal diet
(FM) and the mixed diet comprising soybean meal and
rapeseed meal (SM). This study sheds new light on the
regulation of GS in teleost fish and provides new
perceptions and strategies for the formulation of
high-quality feed for triploid crucian carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jhan:2022:PCD,
author = "Hao-Tang Jhan and Hsin-Ta Lee and Kuo-Huan Ting",
title = "The Potential Compatibility of Designating Offshore
Wind Farms within Wider Marine Protected
Areas---Conservation of the \geoname{Chinese} White
Dolphin Regarding Fishers' Perception",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "195",
day = "05",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040195",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/195",
abstract = "The population of the Chinese white dolphin along
Taiwan's west coast is under a range of threats. The
designation of marine protected areas (MPA) is urgently
required for their protection. However, conflicts
between specific species conservation and fishing
rights mean that the success of such a designation
relies on the fishers' perceptions and awareness of an
MPA. Designating offshore wind farms within MPAs can be
a mechanism for minimizing conflicts between fisheries
and conservation. The purpose of this study is to
examine the potential for designating an offshore wind
farm within an MPA for Chinese white dolphin
conservation by exploring the attitudes of local
fishers. This study used face-to-face questionnaires.
The results show that the main challenges are conflicts
of interest, insufficient science-based information,
and inadequate law enforcement. Offshore wind farms
could be a way to maximize the benefits for different
stakeholders and positively impact the marine
environment and ecosystem. This study makes feasible
recommendations on how to improve conservation, promote
renewable energy, and encourage sustainable
fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lopes:2022:DEF,
author = "Ana F. Lopes and Robyn Murdoch and Sara
Martins-Cardoso and Carolina Madeira and Pedro M. Costa
and Ana S. F{\'e}lix and Rui F. Oliveira and Narcisa M.
Bandarra and Catarina Vinagre and Ana R. Lopes and
Emanuel J. Gon{\c{c}}alves and Ana Margarida Faria",
title = "Differential Effects of Food Restriction and Warming
in the Two-Spotted Goby: Impaired Reproductive
Performance and Stressed Offspring",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "194",
day = "05",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040194",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/194",
abstract = "Climate change is a growing threat to marine organisms
and ecosystems, and it is already modifying ocean
properties by, for example, increasing temperature and
decreasing pH. Increasing water temperature may also
lead to an impairment of primary productivity and an
overall depletion of available zooplankton.
Understanding how the crossover between warming and
zooplankton availability impacts fish populations has
paramount implications for conservation and mitigation
strategies. Through a cross factorial design to test
the effects of ocean temperature and food availability
in a temperate marine teleost, Pomatochistus
flavescens, we showed that hindered feeding impacted
sheltering and avoidance behaviour. Also, low food
availability impaired fish reproduction, particularly
male reproduction, as the expression of cyp11b1, a gene
with a pivotal role in the synthesis of the most
important fish androgen, 11-ketotestosterone, was
significantly reduced under a low food regime. In
contrast, temperature alone did not affect reproductive
success, but offspring showed increased saturated fatty
acid content (embryos) and increased lipid peroxidation
(larvae). Altogether, food availability had a stronger
effect on fitness, showing that coping with elevated
temperatures, an ability that may be expected in
shallow-water fish, can be indirectly impacted, or even
overwhelmed, by the effects of ocean warming on primary
productivity and downstream ecological processes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2022:CDS,
author = "Haoyu Li and Xiaonan Zhang and Yang Zhang and Qi Liu
and Fengwen Liu and Donglin Li and Hucai Zhang",
title = "Climate-Driven Synchrony in Anchovy Fluctuations: A
\geoname{Pacific}-Wide Comparison",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "193",
day = "05",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040193",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/193",
abstract = "Fish population fluctuations have been widely
documented and are often attributed to climate
variability, but little is known about their
relationship. This is a barrier that restricts our
capacity to understand climate effects on fish
fluctuations. Here, we collected published data to
conduct a comparative analysis of time series in terms
of both scale deposition rate (SDR) and catch to
indicate the abundance of anchovy and explored its
relationships with climatic and environmental
parameters. We first conducted a Spearman correlation
analysis to identify the differences in the SDR between
sites. Then, we used Generalized Additive Models to
evaluate the effects of external forcing at multiple
spatial scales (i.e., local and global scales) on the
anchovy catches recorded at the California Current
Ecosystem (CCE), Humboldt Current Ecosystem (HCE), and
Kuroshio Current Ecosystem (KCE). We found that the
SDRs at different sites in the same Current Systems are
generally consistent, while there are more differences
between different systems. We also found that the
Pacific Decadal Oscillation index (PDOI), North Pacific
index (NPI), North Pacific Gyre Oscillation index
(NPGOI), Sea Surface Temperature anomaly (SSTA), and
Air Temperature anomaly (ATA) were the most influential
factors explaining the variability in anchovy catch for
CCE, whereas the NPGOI was the most influential factor
for HCE. The PDOI was the best at explaining the
variability in anchovy catch for KCE. Our results
suggest that anchovy fluctuations in the Pan-Pacific
occurred synchronously. These seemingly unrelated
events, which occurred thousands of kilometers apart,
were actually not isolated. Therefore, we suggest that
large-scale climate forcing may activate synchronous
fluctuations for anchovy populations at the basin
scale, whereas local-scale environmental forces are
also responsible for anchovy fluctuations in the
``micro-environment''.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2022:ACE,
author = "Peiwen Wang and Isabel Mendes",
title = "Assessment of Changes in Environmental Factors
Affecting Aquaculture Production and Fisherfolk Incomes
in {China} between 2010 and 2020",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "192",
day = "05",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040192",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/192",
abstract = "The vast expanse of China's land surface results in
the country's environment varying from region to
region. Environmental changes impact on China's
industries, markets, and trade, indirectly affecting
not only the country's economy but also the people who
depend on aquaculture resources. Regional
differentiation leads to an imbalance that severely
affects social fairness and equity, which becomes a key
factor limiting the sustainable development of the
economy and society. Analysis and assessment of the
changes in environmental factors affecting aquaculture
production and fisherfolk's income in 31 regions of
China between 2010 and 2020 aim to provide a reference
for regional differentiation in the economic
development of aquaculture in the different regions in
China, representing an essential step towards achieving
the coordinated development of rural regional areas.
This study's assessment and analysis procedures adopted
the principal component analysis method. The findings
suggest that regional differences in Chinese
fisherfolk's income and the environmental factors
affecting China's aquaculture production are veritable.
There have been subtle changes in regional
differentiation over a decade. It is necessary to
implement contextualized environmental management
measures, concessionary taxation, and additional
subsidies to address the different characteristics of
China's different regions for the future development of
environmental management and narrowing the income gap,
to address both the income disparities in Chinese
fisherfolk's income and environmental factors affecting
Chinese aquaculture production, to achieve the
harmonious development of rural regional areas.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2022:MCS,
author = "Xiaojuan Hu and Haochang Su and Peng Zhang and Zuozhi
Chen and Yu Xu and Wujie Xu and Jie Li and Guoliang Wen
and Yucheng Cao",
title = "Microbial Community Structure and Metabolic
Characteristics of Intestine and Gills of Dwarf-Form
Populations of \bioname{Sthenoteuthis oualaniensis} in
\geoname{South China Sea}",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "191",
day = "04",
month = aug,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040191",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/191",
abstract = "Sthenoteuthis oualaniensis is an important biological
resource in the South China Sea. However, the
microbiological characteristics of this squid,
especially those of the dwarf-form, are poorly
understood. This study was conducted to analyze the
microbial community structure and metabolic
characteristics of the intestinal and gill tissues of
dwarf-form populations of S. oualaniensis. The
dwarf-form squids of different sexes and gonadal
maturities were collected from South China Sea in
spring 2020. Results showed that Mycoplasma was the
most dominant group of bacteria in the intestinal
samples of the females with immature gonads (FN),
females at sexual maturity (FY), and males at sexual
maturity (MY) and the second-highest relative abundance
group in males with immature gonads (MN). The microbial
community structure in squid gills differed from that
of intestinal flora. The BD1-7 clade was the dominant
genus in gill samples of all groups. Furthermore, the
microbial community activities in gills were higher
than in intestinal groups, especially FYG. The larger
dwarf-form populations had microbial communities with
more robust utilization of carbon sources, assessed via
average well color development (AWCD). Correlation and
redundancy analysis determined that AWCD significantly
positively correlated with the relative abundance of
BD1-7 clade ($ p < 0.05$). The results indicated that
the dominant group of bacteria and microbial community
structure were different between the intestinal and
gill microbial communities in the dwarf-form S.
oualaniensis populations of different sexes and
maturities. Moreover, the metabolic potential of the
gill microbial community was higher than that of the
intestinal microbial community in the dwarf-form
populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Subhan:2022:EUB,
author = "Ujang Subhan and Iskandar and Zahidah and Camellia
Panatarani and I Made Joni",
title = "Effect of Ultrafine Bubbles on Various Stocking
Density of Striped Catfish Larviculture in
Recirculating Aquaculture System",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "190",
day = "29",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040190",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/190",
abstract = "The effects of ultrafine bubbles on the high stock
density of striped catfish larvae in a recirculating
aquaculture system (RAS) are described in this research
(UFBs-RAS). In this study, the various stock densities
of striped catfish were investigated regarding the
effect of oxygen saturation on the yolk sac absorption
rate, length growth rate, and yolk sac utilization
efficiency at the endogenous stage. The survival rate,
the specific growth rate (weight, length, and biomass),
and the gross feeding efficiency were examined at an
exogenous stage. The results showed that the ultrafine
bubbles generator in the recirculating aquaculture
system (UFBs-RAS) provide the dissolved oxygen
concentration up to 128.97\%sat. The oxygen saturated
state in FBs-RAS at the stock density 100 fish/L (D100)
provided high yolk sac utilization efficiency in the
endogenous stage and high survival, specific growth
rate, and gross feeding efficiency in the exogenous
stage. It was emphasized that the performance was
possible due to surplus oxygen up to 1.58 mg/L at the
stock density of 100 fish/L and accomplished minimum
ammonia (NH 3 -N) content much lower than the limit
(0.12 {\SGMLmicro}g/L). Thus, the striped catfish
larviculture with UFBs-RAS-provided oxygen balance
subsequently improved the production rate significantly
with cost-effective production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sumon:2022:EPA,
author = "Md Afsar Ahmed Sumon and Mohammad Habibur Rahman Molla
and Israa J. Hakeem and Foysal Ahammad and Ramzi H.
Amran and Mamdoh T. Jamal and Mohamed Hosny Gabr and
Md. Shafiqul Islam and Md. Tariqul Alam and Christopher
L. Brown and Eun-Woo Lee and Mohammed Moulay and Amer
H. Asseri and F A Dain Md Opo and Ahad Amer Alsaiari
and Md. Tawheed Hasan",
title = "Epigenetics and Probiotics Application toward the
Modulation of Fish Reproductive Performance",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "189",
day = "28",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040189",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/189",
abstract = "Fish represent an excellent source of animal protein
as well as a biomedical research model as a result of
their evolutionary relatedness and similarity with the
human genome. Commercial and ornamental fish culture
has achieved popularity, but reproductive dysfunctions
act as a limiting factor for quality fry production,
interfering with the sustainability of the aquaculture
industry. Fish reproduction is crucial for any species'
existence, and reproductive performance can potentially
be improved through applications of epigenetics and
probiotics. Epigenetics is a highly sensitive molecular
approach that includes chromatin structure and function
alteration, DNA methylation, and modification of
non-coding RNA molecules for the transfer of desired
information from parents to offspring. DNA
methyltransferase improves reproductive cyp11a1, esr2b,
and figla gene expression and feminizes zebrafish
(Danio rerio). Moreover, epigenetics also contributes
to genome stability, environmental plasticity, and
embryonic development. However, methylation of specific
genes can negatively affect sperm quality, resulting in
poor fertilization. Probiotic administration is able to
induce responsiveness of incompetent follicles to
maturation-inducing hormones and can change oocyte
chemical composition during vitellogenic development.
The positive role of probiotics on testicular cells is
validated by upregulating the transcription levels of
leptin, bdnf, and dmrt1 genes facilitating the
spermatogenesis. This review not only discusses the
effects and mechanism of epigenetics and probiotics for
improving fish reproduction, but also presents an
overview of the causal factors and current techniques
used to eradicate dysfunction. Moreover, key genes and
hormones related to fish reproduction along with
research gaps and future prospects are also considered.
This review provides an overview of necessary
information for students, scientists, researchers, and
breeders to resolve fish reproduction-related problems
to ensure profitable and sustainable aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dominguez-Petit:2022:PER,
author = "Rosario Dom{\'\i}nguez-Petit and Cristina
Garc{\'\i}a-Fern{\'a}ndez and Ezequiel Leonarduzzi and
Karina Rodrigues and Gustavo Javier Macchi",
title = "Parental Effects and Reproductive Potential of Fish
and Marine Invertebrates: Cross-Generational Impact of
Environmental Experiences",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "188",
day = "27",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040188",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/188",
abstract = "The reproductive success of aquatic animals depends on
a complex web of relationships between the environment,
the attributes of the reproductive individuals and
human-induced selection. All of them are manifested
directly or indirectly through parental effects, which
can also compensate for certain external impacts.
Parental effects refer to the influence that the
phenotype and environmental conditions in which
individuals develop exert on the phenotype of their
offspring, and they can even have transgenerational
impact. This paper describes the different types of
parental effects and reviews the published literature
to analyze the causes of their variation and their
impact on reproductive resilience and population
dynamics.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2022:BME,
author = "Ziyue Chen and Huajie Lu and Wei Liu and Kai Liu and
Xinjun Chen",
title = "Beak Microstructure Estimates of the Age, Growth, and
Population Structure of Purpleback Flying Squid
(\bioname{Sthenoteuthis oualaniensis}) in the
\geoname{Xisha Islands} Waters of the \geoname{South
China Sea}",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "187",
day = "26",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040187",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/187",
abstract = "This study aimed to explore the feasibility of using
an upper beak microstructure to estimate the age of
purpleback flying squid (Sthenoteuthis oualaniensis).
From these microstructures, the age and growth of squid
caught from January to March and May to August in 2018,
2019, and 2020 in the waters surrounding the Xisha
Islands in the South China Sea were determined. We
found three typical growth zones (the hood region,
crest region, and axis), abnormal increments (checks),
and erosion in the beak examination. The average dorsal
mantle length (ML) of males and females was 112.13
({\pm}15.23 mm) and 119.67 mm ({\pm}24.50 mm),
respectively, and no squid were older than 10 months.
The peak hatching dates, according to back
calculations, were from October to January of the next
year. All sampled squid belonged to the autumn/winter
cohort. Significant sex differences were found in the
relationship between ML and age in squid with similar
growth patterns. Exponential models best described the
relationships of ML with age and body weight (BW) for
both sexes. However, a linear model best described the
relationship between age and upper rostrum length
(URL). The maximum absolute daily growth rates (AGR) of
BW were reached during days 240--270 for both sexes.
The maximum AGRs in ML were reached during days
180--210 and 240--270 for males and females,
respectively. The period of 120--150 days (4--5 months)
was considered the sub-adult stage of S. oualaniensis
in the Xisha Islands waters of the South China Sea.
This study confirmed that the beak microstructure
provides good age estimates for purpleback flying squid
(S. oualaniensis).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Eegunjobi:2022:DGV,
author = "Ruth Eegunjobi and Nicholas Ngepah",
title = "The Determinants of Global Value Chain Participation
in Developing Seafood-Exporting Countries",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "186",
day = "26",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040186",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/186",
abstract = "Global value chain (GVC) participation has played a
significant role in boosting the trade gains of both
developed and developing seafood-exporting countries
over the past three decades. In addition, the extent of
GVC participation has become the most important
platform for addressing gains from trade in developing
seafood-exporting countries to ensure that their
participation enhances economic growth. Recent studies
on GVC participation in developing countries have
highlighted the importance of domestic institutions.
However, the literature is silent on the quality of the
domestic institutions--GVC participation nexus. This
paper aims to investigate the determinants of GVC
participation and the effect of the quality of domestic
institutional governance on seafood-exporting
developing countries' GVC participation indices. Using
the Hausman--Taylor (HT) estimator and the system
generalised method of moments (GMM) dynamic panel data
methodology to examine seafood export data from 32
countries from 2009 to 2018, we find that economic
potential drives backward GVC participation, while low
forward participation might not only lead to lower
gains from trade, but also limit countries to the
supply of primary seafood products with little value
addition. In addition, the quality of domestic
institutional governance constrains GVC participation.
Overall the results indicate that the quality of
domestic institutional governance matters for the GVC
participation of seafood-exporting developing
countries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Araujo:2022:DAA,
author = "Bruno Cavalheiro Ara{\'u}jo and Arleta Krystyna
Skrzynska and Victor Hugo Marques and Aurora Tinajero
and Oscar Bas{\'\i}lio {Del Rio-Zaragoza} and Maria
Teresa Viana and Jos{\'e} Antonio Mata-Sotres",
title = "Dietary Arachidonic Acid (20:4n-6) Levels and Its
Effect on Growth Performance, Fatty Acid Profile, Gene
Expression for Lipid Metabolism, and Health Status of
Juvenile \geoname{California} Yellowtail
(\bioname{Seriola dorsalis})",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "185",
day = "25",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040185",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/185",
abstract = "Arachidonic acid (ARA, 20:4n-6) fed to Seriola
dorsalis juveniles at different levels was evaluated.
After a seven-week feeding trial, growth performance,
hepatopancreas and muscle fatty acid (FA) composition,
expression of lipid-relevant genes, and blood
parameters were evaluated. Four isoproteic and
isolipidic experimental diets were formulated to
contain 44\% crude protein and 11\% lipids with graded
inclusion levels of ARA, 0\% (Control), 0.4, 0.9, and
1.4\% of the total diet. S. dorsalis juveniles (14.54
{\pm} 0.18 g) were randomly divided into twelve tanks
with fifteen animals each. The animals were hand fed
three times per day to apparent satiation. Dietary
treatments did not significantly affect the growth
performance, SGR, FCR, and feed intake of fish.
Different levels of ARA in the experimental diets
directly influenced liver and muscle FA profiles, with
significant changes in ARA and EPA deposition between
Control treatment and 1.4\%, in both tissues. The
expression of arachidonate 5-lipoxygenase (alox5),
acyl-CoA dehydrogenase very long chain (acadvl),
carnitine O-palmitoyltransferase 1(cpt1a) was
significantly affected by dietary treatments, with an
expression increasing accordingly to the increasing ARA
levels. In contrast, a reduction of fatty acid synthase
(fas) and proliferator-activated receptor alpha (ppara)
expression was significantly reduced as ARA increased
in the diet. In addition, a significant reduction in
blood cortisol and glucose was found at a 0.9\% ARA
level compared to the other treatments. Based on the
performance, cortisol levels, the gene expression for
eicosanoids synthesis, and lipid metabolic pathways,
the present study suggests a maximum ARA inclusion of
0.9\% in diets for California yellowtail juveniles, S.
dorsalis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kindong:2022:LBA,
author = "Richard Kindong and Ousmane Sarr and Feng Wu and
Siquan Tian",
title = "Length-Based Assessment Methods for the Conservation
of a Pelagic Shark, \bioname{Carcharhinus falciformis}
from the \geoname{Tropical Pacific Ocean}",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "184",
day = "25",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040184",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/184",
abstract = "The silky shark, Carcharhinus falciformis is one of
the most heavily exploited sharks, being the main
by-catch species in both tuna longline and purse-seine
fisheries in tropical waters worldwide. Despite this
severe exploitation, little is known about the species'
life history and population status. Silky sharks, like
many other sharks, exhibit slow growth and low
fecundity, indicating the urgency of developing
assessment studies to aid in the implementation of
conservation plans for their stocks. Because
information on the catch and effort of this species is
scarce, some length-based data-limited methods were
applied in the present study to provide estimates of
the status of the tropical Pacific silky shark
population. As evident from the LBSPR analysis, the
current spawning potential ratio (SPR) was found to be
below the target reference point of SPR 40\% and
slightly above the limit reference point of SPR 20\%.
In addition, the LBB model also confirmed that this
stock's status is overfished with relatively low
biomass levels. Furthermore, both models showed
estimates of size selectivity at 50\% and 95\% that
were lower than the estimated size at sexual maturity.
In conclusion, the data-limited models developed in
this study indicated that the silky shark stock in the
tropical Pacific Ocean may be at risk of further
decline. Additionally, the results show that growth and
recruitment overfishing may be occurring in the silky
shark's population calling for immediate
intensification of monitoring programs for these sharks
as a pre-requisite to develop efficient management and
conservation plans in the Pacific Ocean.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Harris:2022:EAT,
author = "Michaela Harris and Nathalie Brodeur and Francis
LeBlanc and Scott Douglas and Paul Chamberland and
Thomas Guyondet and Royce Steeves and Nellie
Gagn{\'e}",
title = "{eDNA} and Acoustic Tag Monitoring Reveal Congruent
Overwintering Distributions of Striped Bass in a
Hydrologically Complex Estuarine Environment",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "183",
day = "23",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040183",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/183",
abstract = "After collapsing in the late 1990s, the southern Gulf
of St. Lawrence population of striped bass (Morone
saxatilis) is recovering. Here, we evaluate the use of
under-ice eDNA sampling to monitor the population and
confirm overwintering locations. From 2018 to 2020,
water samples were collected from transects spanning 35
km of the Miramichi River system, accounting for the
effects of sampling site, month, sampling depth and
tidal influence on eDNA concentration. We examined the
distribution of eDNA in a complex tidal river system
with a time series consisting of 12 h of continuous
sampling throughout a tidal cycle, in conjunction with
the use of artificial DNA tracers and acoustic Doppler
current profiler flow measurements. The eDNA
distribution correctly identified overwintering grounds
based on acoustic tag data, including a perceptible
upstream shift in 2020. Overall, there was no
significant effect of year, sampling month (February or
March), sampling depth or tidal phase on eDNA
concentrations. The tidal time series revealed only
weak patterns of eDNA recirculation. Monitoring eDNA
concentration and distribution allows for a relative
comparison of population size and location between
years, and has the potential to be expanded to other
river systems more easily than traditional acoustic
fish tags and surveys.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2022:PGD,
author = "Shihui Wang and Liang Luo and Rui Zhang and Kun Guo
and Wei Xu and Zhigang Zhao",
title = "Population Genetic Diversity and Differentiation of
Mitten Crab, Genus \bioname{Eriocheir}, Based on
Microsatellite Markers",
journal = j-FISHES,
volume = "7",
number = "4",
pages = "182",
day = "22",
month = jul,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7040182",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:12 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/4/182",
abstract = "Mitten crab, Eriocheir sensu stricto, is an important
indigenous aquatic species and food source in Eastern
Asia. Genetic diversity is an important prerequisite
for the conservation and utilization of germplasm
resources. However, the genetic diversity and
differentiation of Eriocheir s. s. remains unclear.
This study evaluated population genetic diversity and
genetic differentiation of three Eriocheir s. s.
populations from Suifenhe (SFH), Liaohe (LH), and
Nanliujiang (NLJ), China, based on 19 microsatellite
markers. The mean observed number of alleles (N a) was
22.84 alleles, and the mean polymorphism information
content (PIC) was 0.86, which demonstrated high genetic
diversity. The allele frequency distribution showed an
``L'' shape, ranging from 0.01 to 0.74. The genetic
diversity parameter values of the LH population were
higher than those of the other two populations. All
pairwise F ST values showed significant differences
among the three Eriocheir s. s. populations (p {$<$}
0.01). The value of Nei's genetic distance (D S) varied
from 0.31 (between SFH and NLJ populations) to 0.33
(between SFH and LH populations). Overall, The results
illustrate that the three wild Eriocheir s. s.
populations have high genetic diversity and high
genetic differentiation. This study provides the basis
for Eriocheir s. s. utilization in China.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2022:EEE,
author = "Guobin Chen and Tong Zhou and Meng Chen and Guiwei Zou
and Hongwei Liang",
title = "Effect of Estradiol on Estrogen Nuclear Receptors
Genes Expression on Embryonic Development Stages in
\geoname{Chinese} Soft-Shelled Turtle
(\bioname{Pelodiscus sinensis})",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "223",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050223",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/223",
abstract = "Among Chinese soft-shelled turtles, Pelodiscus
sinensis, males have a richer nutritional value and
higher market price than females. All-male offspring
were obtained by 17{\beta}-estradiol (E2). However, the
molecular mechanisms of E2 inducing sexual reversal
remain unclear. In this study, we cloned estrogen
nuclear receptors (ERs) from P. sinensis and
investigated their expression profiles. We examined the
responses of ER{\alpha} and ER{\beta} after treatment
with different concentrations of 1.0, 5.0, and 10 mg/mL
E2. ERs showed abundant expressions in the adult gonad,
ER{\alpha} for ovary, and ER{\beta} for testis. E2 can
up-regulate the expression of ER{\alpha}, which showed
a remarkable increase while the promotion of ER{\beta}
was unobvious. They reached a high level at stage 17
after the treatment of E2, genes of the female-related
genes Rspo1, Wnt4, {\beta}-catenin, Foxl2, Cyp19a1, and
Sox3 exhibited a significant raise at stage 17 with the
increase in the concentration of E2 while the
male-related genes Sox9, Dmrt1, and Amh were
significantly inhibited. Our study cloned the full
length of ERs and analyzed their structures and
expressions, laying a foundation for the further study
of the effect of estrogen on sex determination.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2022:FSR,
author = "Yin Liu and Lun Song and Guangjun Song and Jinhao Wu
and Kun Wang and Zhaohui Wang and Suxuan Liu",
title = "Feeding Selectivity of \bioname{Ruditapes
philippinarum} on Phytoplankton",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "222",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050222",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/222",
abstract = "A monthly survey of the feeding selectivity of
Ruditapes philippinarum in the Yalu River Estuary in
2020--2021 was conducted using high-throughput
sequencing identification and visual grading
technology. The results showed that the most-dominant
species in the water of the shellfish culture area and
in the stomachs of R. philippinarum was Karlodinium
veneficum in those years. The selectivity index (E)
indicated that R. philippinarum avoided consuming
Bacillariophyta, Chrysophyta and Cryptophyta throughout
the year and preferentially consumed Dinophyta and
Chlorophyta. In 2020, the annual average biomass of
Dinophyta, Bacillariophyta, Chlorophyta,
Dictyochophyta, Cryptophyta and Chrysophyta in the
stomach contents of R. philippinarum was
54:14:16:1:10:4; it was 41:12:28:0:1:17 in 2021. The
annual average biomass ratio of picophytoplankton,
nanophytoplankton and microphytoplankton in the
stomachs of R. philippinarum was 13:48:39 in 2020; it
was 14:66:20 in 2021. R. philippinarum actively fed on
nanophytoplankton and avoided picophytoplankton. Among
the phytoplankton of different sizes and groups that R.
philippinarum prefer to feed, chemical oxygen demand
(COD) and organic phosphorus (DOP) have a significant
negative effect on the nanophytoplankton community, pH
has a positive effect on the Dictyochophyta community
and COD and the inorganic nitrogen to phosphorus ratio
(DI-N/P) have a significant positive effect on the
Chlorophyta community.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2022:FAP,
author = "Guanyu Hu and Zhenfang Zhao and Bilin Liu and Dongming
Lin and Jiawei Liang and Zhou Fang and Xinjun Chen",
title = "Fatty Acid Profile of Jumbo Squid (\bioname{Dosidicus
gigas}) off the {Peruvian Exclusive Economic Zone}:
Revealing the Variability of Feeding Strategies",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "221",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050221",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/221",
abstract = "Jumbo squid Dosidicus gigas play a critical role in
the marine ecosystems and are an important commercial
species in the East Pacific. Generally, three size
groups have been distinguished on the basis of the
mantle length (ML) of an adult. Here, fatty acid (FA)
analyses of muscle tissues were used to explore the
feeding strategies of D. gigas off the Peruvian
exclusive economic zone in terms of different size
groups and sexes. There was no significant difference
in fatty acid composition between the small- and
medium-sized groups, whereas the large-sized group
differed significantly from other groups. The higher
content of C20:4n6 and (C18:2n6 + C18:3n3) indicates
that the large-sized group may feed more frequently in
nearshore and deep waters. Furthermore, the niches of
the three size groups were consistent with the results
of fatty acid composition, with the large-sized group
occupying the widest trophic niche, followed by the
medium-sized group and then the small-sized group. In
addition, there was evidence of trophic niche overlap
between the small and medium groups. In terms of sexual
variability of the small and medium groups, the fatty
acid composition significantly differed between females
and males. In terms of sexual variation of the large
group, the fatty acid composition between females and
males was similar, indicating that similar feeding
strategies may be adopted by them. This study revealed
the variability of the feeding strategies of three size
groups of D. gigas off the Peruvian exclusive economic
zone.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Imsland:2022:LWT,
author = "Albert Kjartan Dagbjartarson Imsland and Patrick
Reynolds",
title = "In lumpfish We Trust? {The} Efficacy of Lumpfish
\bioname{Cyclopterus lumpus} to Control
\bioname{Lepeophtheirus salmonis} Infestations on
Farmed \geoname{Atlantic} Salmon: a Review",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "220",
day = "25",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050220",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/220",
abstract = "In this review, we have systematized current knowledge
about the effect of stocking lumpfish (Cyclopterus
lumpus) as cleaner fish to control Lepeophtheirus
salmonis infestations on farmed Atlantic salmon
(\bioname{Salmo salar} {L.}). The review was prompted
by recent reports in which the usefulness of lumpfish
has been doubted, and the urgent need to investigate
whether common lumpfish can be used to reduce L.
salmonis numbers on farmed Atlantic salmon by active
grazing on this species. Available published data
clearly indicate that lumpfish graze on L. salmonis,
and can significantly lower the lice burden in Atlantic
salmon farming. It is possible to enhance the lice
grazing behavior of lumpfish with the assistance of
live feed conditioning prior to sea pen transfer, and
with selective breeding. Data indicate that lice
grazing of lumpfish is size dependent, and grazing
effect is low for lumpfish larger than 200--250 g.
Observations from large-scale rearing of Atlantic
salmon in open sea cages in Norway, Iceland, the Faroe
Islands, and Scotland also indicate that lumpfish can
be effective in lowering infestations of L. salmonis on
salmon. Overall, this present review reveals that
lumpfish can actively contribute to lower numbers of L.
salmonis on farmed Atlantic salmon.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2022:FFI,
author = "Danyang Li and Houcheng Su and Kailin Jiang and Dan
Liu and Xuliang Duan",
title = "Fish Face Identification Based on Rotated Object
Detection: Dataset and Exploration",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "219",
day = "25",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050219",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/219",
abstract = "At present, fish farming still uses manual
identification methods. With the rapid development of
deep learning, the application of computer vision in
agriculture and farming to achieve agricultural
intelligence has become a current research hotspot. We
explored the use of facial recognition in fish. We
collected and produced a fish identification dataset
with 3412 images and a fish object detection dataset
with 2320 images. A rotating box is proposed to detect
fish, which avoids the problem where the traditional
object detection produces a large number of redundant
regions and affects the recognition accuracy. A self-SE
module and a fish face recognition network (FFRNet) are
proposed to implement the fish face identification
task. The experiments proved that our model has an
accuracy rate of over 90\% and an FPS of 200.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2022:EST,
author = "Xingxing Zhou and Zuozhi Chen and Pengli Xiong and
Yancong Cai and Jie Li and Peng Zhang and Jun Zhang and
Miao Li and Jiangtao Fan",
title = "Exploring the Spatial and Temporal Distribution of
Frigate Tuna (\bioname{Auxis thazard}) Habitat in the
\geoname{South China Sea} in Spring and Summer during
2015--2019 Using Fishery and Remote Sensing Data",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "218",
day = "25",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050218",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/218",
abstract = "Frigate tuna Auxis thazard fishery is considered a
potential marine resource in the open South China Sea
(SCS). However, little is known about the spatial and
temporal distribution of its habitat, and how this may
respond to environmental changes. Using fish survey and
remote sensing data from 2015--2019, we applied
generalized additive models to identify relationships
between environmental factors and the distribution of
A. thazard in the SCS. To examine seasonal patterns in
the habitat of A. thazard in the SCS, we generated a
habitat suitability index model using environmental
factors screened by generalized additive models.
Results showed that A. thazard migrates from south to
north in the SCS, and its suitable habitat is patchily
distributed. Significant environmental factors
affecting the habitat of A. thazard distribution were
different in different seasons; we demonstrated A.
thazard to be sensitive to Chl-a in spring (optimum
0.155, optimal range $ \approx $0.1252--0.1840), and in
summer to be sensitive to SST (optimum 30.405, optimal
range $ \approx $29.789--31.021) and SSH (optimum
0.741, optimal range $ \approx $0.618--0.864). Suitable
habitat in spring occurs mainly in northeastern areas,
while in summer it occurs mainly around the
southeastern Nansha Islands. Compared with that in
spring, the area of suitable habitat increases in
summer, as does the habitat suitability index of the
entire sea area. These results improve our
understanding of environmental factors that affect the
distribution of A. thazard habitat in the SCS, and
provide a scientific basis for the development and
management of A. thazard resources.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rabbane:2022:TEA,
author = "Md. Golam Rabbane and Md. Alamgir Kabir and Md.
Habibullah-Al-Mamun and Md. Ghulam Mustafa",
title = "Toxic Effects of Arsenic in Commercially Important
Fish Rohu Carp, \bioname{Labeo rohita} of
\geoname{Bangladesh}",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "217",
day = "25",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050217",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/217",
abstract = "This study was designed to elucidate the effects of
arsenic (As) on the morpho-behavior, growth development
and molecular mechanisms of a commercially important
fish, rohu carp, Labeo rohita, in Bangladesh. Fish fry
with an average weight of 387.5 {\pm} 169.25 mg and an
average length of 3.35 {\pm} 0.37 cm were collected
from a local hatchery in Mymensingh, Bangladesh and
acclimatized for a week in the Department of Fisheries,
University of Dhaka before starting the exposure with
arsenic. Fishes were exposed for a period of 14 days
with three treatments of NaAsO 2, namely treatment
1(T1)---2.5 mg/L; treatment 2 (T2)---15 mg/L; and
treatment 3 (T3)---30 mg/L, along with a control
(C)---0.0 mg/L, with three replicates. These
concentrations were determined based on the LC 50 value
for 96 h measured for this experiment. This study
revealed remarkable morphological abnormalities and
deformities in arsenic-exposed rohu carp. In fish
exposed to 30 mg/L, caudal fin erosion was a frequent
deformity. There was no significant difference in
RNA:DNA ratio among the treatments. The overall weight
of fish was decreased as the concentration of arsenic
was increased. The T3 fish had a statistically
significant negative weight gain ({$-$0}.05 {\pm} 0.07
g), but the other treatments (T1 and T2) and control
fish had no significant weight gain. Different types of
histopathological changes were observed in the gills
and intestines of arsenic-treated fish. Necrosis and
severe damages were found in the secondary lamellae of
gills at the highest arsenic concentration (30 mg/L).
Epithelial lifting, irregular shape and damages in the
gill raker were also observed in the primary lamellae
of the gills for the same treatment. In this study, the
expression of heat shock protein (HSP 60) and
metallothionein (MT) genes was assessed by qPCR, and
these genes were upregulated in different treatments
compared to controls. The findings of the present study
suggest that arsenic pollution significantly changes
the morphology, behavior, growth, development,
histopathology and molecular mechanisms of this
economically important fish, rohu carp, in
Bangladesh.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2022:RHT,
author = "Yan Zhou and Yanjie Zhang and Shang Wei and Wei Li and
Wenhao Li and Zhichao Wu and Shouwen Jiang and Ying Lu
and Qianghua Xu and Liangbiao Chen",
title = "Reduced Hypoxia Tolerance and Altered Gill Morphology
at Elevated Temperatures May Limit the Survival of
Tilapia ({GIFT}, \bioname{Oreochromis niloticus}) under
Global Warming",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "216",
day = "24",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050216",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/216",
abstract = "Nile tilapia (Oreochromis niloticus) is one of the
most important food fishes in global aquaculture. The
optimal rearing temperature for Nile tilapia is 27--30
{$^\circ $}C; however, in some Asian breeding areas,
such as south China, water temperatures in summer
frequently exceed 35 {$^\circ $}C for several days.
Potential effects of long-term exposure to high
temperatures on the survival and metabolism of tilapia
are unclear. In this study, genetically improved farmed
tilapia, age six weeks, were exposed to water
temperatures of 28, 32, and 36 {$^\circ $}C for 15
weeks. Mean survival rates and tolerance to hypoxia
were significantly reduced, and respiratory rates were
increased in fish reared at 36 {$^\circ $}C, compared
to the 28 and 32 {$^\circ $}C treatments (p {$<$}
0.05). Partial temperature compensation for routine
metabolic rates was observed after 15 weeks at 36
{$^\circ $}C. Gill morphology changes in the 36
{$^\circ $}C treatment included curling of the lamellae
and hyperplasia of the filament end, which became more
pronounced after acute hypoxia (0.2 mg/L O 2).
Transcriptomics demonstrated that expression of
numerous genes related to aerobic metabolism was
altered in the 36 {$^\circ $}C treatment, including
down-regulation of nine genes of the tricarboxylic acid
cycle. In summary, high temperature affected tilapia
gill morphology, reduced hypoxia tolerance, and
inhibited aerobic metabolism, thus ultimately
threatening tilapia viability and survival.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lu:2022:AGP,
author = "Huajie Lu and Yuzhe Ou and Yurong Teng and Ziyue Chen
and Xinjun Chen",
title = "Age, Growth and Population Structure Analyses of the
\bioname{Berryteuthis magister shevtsovi} in the
\geoname{Japan Sea} by Statolith Microstructure",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "215",
day = "24",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050215",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/215",
abstract = "Berryteuthis magister shevtsovi is a new subspecies in
the Japan Sea, and thus, little is known about its
fisheries biology, especially age and population
structure. Based on the 296 samples collected by the
Chinese commercial jigging vessel in December 2018 in
the Japan Sea. The age, growth, and population
structure of B. magister shevtsovi were studied based
on the microstructure of the statolith. The results
indicated that the range of mantle length (ML) was
90--148 mm for females and 94--141 mm for males, the
body weight (BW) ranged from 49 to 116 g and 38 to 110
g for females and males, and the ages were estimated
from 52 to 166 days for females and 51 to 143 days for
males, respectively. The hatching date extended from
March to October, with a peak from July to September,
suggesting the presence of one spawning group
(summer--autumn group). The ANCOVA showed that there
was no significant sex difference between the ML and BW
growth; however, there was a significant difference
between the sexes in the relationship between ML--age
and BW--age. The relationship between the ML and BW was
best described by the power function, the ML--age
relationship of females was best described by linear
function; the ML--age relationship of males and BW--age
relationships of females and males were best described
by the exponential function. B. magister shevtsovi is a
high-growing squid, and the growth rate seemed to be
high at the young life stage and decreased after the
subadult stage (60--120 days older). This study
provided basic information on the age, growth, and
population structure of B. magister shevtsovi.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sultana:2022:SAS,
author = "Rokeya Sultana and Qun Liu and Petra Schneider and Md.
Abdullah Al-Mamun and Al Mamun and Md. Farhan Tazim and
Mohammad Mojibul Hoque Mozumder and Mohammed Rashed
Parvej and Md. Mostafa Shamsuzzaman",
title = "Stock Assessment of Six {Sciaenidae} Species in the
\geoname{Bay of Bengal}, \geoname{Bangladesh} Water
Using a {Length-Based Bayesian Biomass (LBB)} Method",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "214",
day = "24",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050214",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/214",
abstract = "Six most abundant and commercially valuable croakers
(Sciaenidae) stocks in the coastal water of Bangladesh
were evaluated using a length-based Bayesian Biomass
(LBB) approach. The ratios B/B 0 (current relative
biomass) were smaller than the B MSY /B 0 in five of
the six stocks. For the six estimated populations, two
(Otolithes ruber and Pterotolithus maculatus) are
grossly overfished, one (Otolithoides pama) is
overfished, two (Johnius belangerii and Panna
heterolepis) are slightly overfished, and only donkey
croaker (Pennahia anea) is in the healthy (B/B 0 {$>$}
B MSY /B 0) status. Furthermore, the optimal length at
first capture (Lc\_opt) was higher than the length at
first capture (Lc) in four populations, indicating
growth overfishing, suggesting that increasing mesh
size would benefit the catch and biomass. Findings from
the present study confirm the declining trend of
fisheries resources, particularly the croaker species
in the BoB, Bangladesh coastal water. Management
strategies (such as effort control, choosing the
appropriate mesh size, total allowable catch limit,
identify and enhance protection of the feeding,
breeding, and nursery ground, etc.) should be taken for
the sustainable management and recovery of the
country's marine fishing resources, particularly the
valuable croaker species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Denis:2022:AGE,
author = "J{\'e}r{\'e}my Denis and K{\'e}lig Mah{\'e} and Rachid
Amara",
title = "Abundance and Growth of the \geoname{European} Eels
(\bioname{Anguilla anguilla} {Linnaeus}, 1758) in Small
Estuarine Habitats from the \geoname{Eastern English
Channel}",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "213",
day = "23",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050213",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/213",
abstract = "Abundance and growth of the European eel from six
small northern French estuaries during their growth
phase were examined to explore variations according to
the local habitat characteristics. The length--weight
relationships and growth models fitted to length-at-age
back-calculated otolith growth increments were used to
compare the growth. Higher abundances were observed in
the smaller estuaries (2.4 to 10.5 ind. fyke nets 24 h
{$-$1}). The eel length ranged from 215--924 mm with an
age range of 4--21 years. There was no significant
difference in fish eel lengths or age except in the
Liane estuary where the individuals were larger. The
length--weight relationships showed an isometric or
positive allometric growth in most estuaries. The
Gompertz growth models, which best fits the growth,
showed no significant differences between estuaries
except for female eels from the Liane and the Somme
estuaries where the growth performance index was
higher. The estimated annual growth rate varied from
2.7 to 115.0 mm{\cdot}yr {$-$1} for female and from 4.4
to 90.5 mm{\cdot}yr {$-$1} for male. The present study
shows that eels in the six estuaries had CPUE and
growth rates similar to those previously reported in
larger habitats. These results reinforce the idea that
small estuaries are important habitats that contribute
significantly to the eel population and, therefore,
play an essential role in conservation strategies for
European eel.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Guo:2022:CEE,
author = "Kun Guo and Zhigang Zhao and Jun Xie and Liang Luo and
Shihui Wang and Rui Zhang and Wei Xu and Xiaoli Huang",
title = "Combined Effects of Eco-Substrate and Carbon Addition
on Water Quality, Fish Performance and Nutrient Budgets
in the Pond Polyculture System",
journal = j-FISHES,
volume = "7",
number = "5",
pages = "212",
day = "23",
month = oct,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7050212",
ISSN = "2410-3888",
bibdate = "Sat Aug 27 12:39:13 MDT 2022",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/5/212",
abstract = "Traditional aquaculture can cause serious
environmental pollution. Biofilm and biofloc technology
have the potential to limit aquaculture pollution. An
outdoor experiment was conducted to evaluate the
combined effects of eco-substrates and carbon addition
on water quality, fish performance and nutrient budgets
in the pond polyculture system. In the treatment group,
the total ammonia nitrogen, nitrate nitrogen, nitrite
nitrogen, and total nitrogen of the water were
significantly lower compared to the control group (p
{$<$} 0.05). The growth performance of H. molitrix and
A. nobilis, including the final individual weight,
specific growth rate, weight gain rate, total
production and net production, was significantly higher
in the treatment groups compared to the control (p
{$<$} 0.05), while there was no significant difference
in those of C. carpio between the two groups. Feed was
the main N ({$>$92}\%) and P ({$>$95}\%) input during
the experiment. Comprehensive accumulation was the main
N ({$>$58}\%) and P ({$>$69}\%) output. The N and P
feed input and comprehensive accumulation output
declined in the treatment group. The N utilization
efficiency in the experimental group increased slightly
($ p < 0.05$), while the P utilization efficiency in
the experimental group was significantly higher
compared to the control ($ p < 0.05$). Therefore, the
application of eco-substrates and carbon addition can
increase water quality, improve fish growth, and
promote nutrient utilization efficiency in pond
polyculture systems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhao:2022:PSR,
author = "Hu Zhao and Jiqin Huang and Cheng Fang and Hongying Ma
and Han Zhang and Jie Deng and Wei Jiang and Fei Kong
and Hongxing Zhang and Hong Liu and Qijun Wang",
title = "Predicting Sex-Related Transcripts in the
\geoname{Chinese} Giant Salamander (\bioname{Andrias
davidianus}): a Transcriptomics Study, Selection Gender
for Preservation, Breeding and Reintroduction",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "399",
day = "19",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060399",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/399",
abstract = "The Chinese giant salamander (\bioname{Andrias
davidianus}) is the biggest extant and endangered
amphibian, which has an incongruous sex ratio during
the artificial breeding process and is sex
indistinguishable in appearance. The sex development
mechanism of this rare species thus needs to be
explored. We therefore conducted this study to explore
the molecular mechanisms of sex hallmarks or gonad
maintenance genes for CGS. In this study, we sequenced
and analyzed the sexually mature male and female gonads
of Chinese giant salamanders (CGSs) using RNA-seq
technology and found a total of 15,063 differentially
expressed genes (DEGs) in the testicular and ovarian
tissues, of which 7973 (52.93\%) were expressed in
testicular and 7090 (47.07\%) in ovarian tissues.
Thirteen gonad DEGs were selected based on gene
expression abundance and quantified in the muscle and
skin tissues of both male and female CGSs. The results
showed that these 13 DEGS, out of the 20 analyzed DEGs,
were expressed in both the muscle and skin tissues and
only two of them (\gene{Bmp15} and \gene{ZP3}) were
consistent in the muscle and skin compared to the
gonads of CGSs of the two sexes. Overall, our study
should provide an important reference for the
sex-characteristics-related genes in this type of
species, shedding new light on future research on this
topic.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Linh:2022:EDR,
author = "Nguyen Vu Linh and Le Thanh Dien and Ha Thanh Dong and
Nuttapon Khongdee and Seyed Hossein Hoseinifar and
Mohamed Saiyad Musthafa and Mahmoud A. O. Dawood and
Hien Van Doan",
title = "Efficacy of Different Routes of Formalin-Killed
Vaccine Administration on Immunity and Disease
Resistance of \geoname{Nile} Tilapia
(\bioname{Oreochromis niloticus}) Challenged with
\bioname{Streptococcus agalactiae}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "398",
day = "19",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060398",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/398",
abstract = "Vaccines prepared from formalin-killed Streptococcus
agalactiae were administered to Nile tilapia
(Oreochromis niloticus) via three different routes:
immersion in a water-based vaccine, injection with an
oil-based vaccine, and as a water-based oral vaccine.
All vaccination treatments increased lysozyme and
peroxidase activity in skin mucus of Nile tilapia by
1.2- to 1.5-fold compared to their activities in
unvaccinated control fish. Likewise, alternative
complement, phagocytosis, and respiratory burst
activities in the blood serum of the vaccinated fish
were 1.2- to 1.5-times higher than in the unvaccinated
fish. In addition, the expression transcripts of
interleukin-1 (IL-1), interleukin-8 (IL-8), and
lipopolysaccharide-binding protein (LBP) were 2.3- to
2.9-fold higher in the vaccinated fish compared to
those in the unvaccinated control. The unvaccinated
fish challenged with Streptococcus agalactiae had a
survival rate of 25\% compared to a survival rate of
78--85\% for the vaccinated fish. The differences
between the unvaccinated and vaccinated fish were all
statistically significant, but there was no significant
difference in any of the indicators of immunity between
the three vaccinated groups. Collectively, these
results confirm that vaccination with formalin-killed
Streptococcus agalactiae significantly improved the
resistance of Nile tilapia to infection by the
pathogen. Overall, the efficacy of oral administration
of the vaccine was comparable to that of vaccine
administered via injection, indicating that oral
vaccination is a viable cost-effective alternative to
administering vaccines by injection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Stathopoulou:2022:AEP,
author = "Paraskevi Stathopoulou and Adamantia Asimaki and
Panagiotis Berillis and Nikolaos Vlahos and Efi Levizou
and Nikolaos Katsoulas and Ioannis T. Karapanagiotidis
and Christos I. Rumbos and Christos G. Athanassiou and
Eleni Mente",
title = "Aqua-Ento-Ponics: Effect of Insect Meal on the
Development of Sea Bass, \bioname{Dicentrarchus
labrax}, in Co-Culture with Lettuce",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "397",
day = "18",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060397",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/397",
abstract = "The aim of this study is to investigate the effect of
10 and 20\% replacement of fishmeal (FM dietary group)
with the superworm Zophobas morio larvae meal (ZM10 and
ZM20 dietary groups) on the growth performance of sea
bass (\bioname{Dicentrarchus labrax}) and lettuce
plants (\bioname{Lactuca sativa}) reared in aquaponics.
Ninety juvenile sea bass with an average body weight of
21.55 g/individual were placed in aquaponic fish tanks,
together with 24 lettuce plants with an average initial
height of 8.90 cm/plant and an average number of leaves
of 5.75/plant over a 45-day trial period. At the end of
that period, all feeding groups exhibited high
survival. In fact, {\Zeta}{\Mu}10 and ZM20 groups
showed similar fish weight gain and specific growth
rate (SGR) ($ p > 0.05$), but significantly lower SGR
($ p < 0.05$) than the FM group. Nevertheless, final
fish body weight and length were similar ($ p > 0.05$)
for all feeding groups. No plant mortality was observed
during the 45-day study period. All three aquaponic
systems resulted in similar leaf fresh weight, as well
as fresh and dry aerial biomass. At the end of the
experimental period, plants in the third system showed
higher fresh leaf weight, total weight of fresh leaves,
total dry aerial biomass, and total produced biomass
compared to the other two systems. The results of the
present study show that fish fed with the ZM10 diet had
higher weight gain (WG) than fish fed with the ZM20
diet, while the SGRs for ZM10 and ZM20 were similar.
Both ZM10 and ZM20 diets result in efficient lettuce
production. This study also provides data that
enlighten the feasibility of integrating insect meals
as fish feeds for aquaponics which helps towards the
development of an ento-aquaponic approach.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dai:2022:FRG,
author = "Chen Dai and Xiaoxin Li and Dapeng Luo and Qingming
Liu and Yun Sun and Zhigang Tu and Minghui Shen",
title = "First Report on Genome Analysis and Pathogenicity of
\bioname{Vibrio tubiashii} {FP17} from Farmed Ivory
Shell (\bioname{Babylonia areolata})",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "396",
day = "17",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060396",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/396",
abstract = "Ivory shell (\bioname{Babylonia areolata}) is a
commercially important aquaculture species mainly found
on the southeast coast of China. However, it has been
greatly affected by vibriosis in recent years. In this
study, FP17 (a potential pathogen) was isolated from a
dying ivory shell with ``acute death syndrome'' and
confirmed as a pathogen via infectious experiment.
Furthermore, phylogenetic analysis based on the average
nucleotide identity (ANI) sequencing of the 16S rRNA
gene and housekeeping genes (\gene{ftsz}, \gene{gapA},
\gene{gyrB}, \gene{mreB}, \gene{pyrH}, \gene{rpoA}, and
\gene{topA}) indicated that FP17 was identical to
Vibrio tubiashii. Transmission electron microscopy
showed that FP17 is curved and has a short rod shape,
with a single flagellum. Besides, the calculated LD 50
after the intramuscular injection of FP17 was 2.11 $
\times $ 10 6 CFU/g at 14 d. The genome of the FP17
strain consists of two chromosomes and one plasmid with
5,261,336 bp and 45.08\% GC content, including 4824
open reading frames (ORFs) and 150 non-coding RNAs
(\gene{ncRNA}). Genome mining revealed that 120
candidate gene clusters, including vibrioferrin and
flagellum-related proteins, are responsible for
virulence. Comparative genomic analysis showed that
vibrioferrin genes, such as pvs and type
\SGMLentity{8549} secretion system protein genes
(\gene{vas}), are specific in \bioname{V. tubiashii}
FP17 but not in the ATCC19109 strain. Furthermore, 92
antimicrobial resistance (AMR) genes, such as
\gene{tufA}, \gene{tet(35)}, \gene{crp}, etc., were
mapped within the genome as the potential candidate for
virulence, consistent with antibiotic susceptibility
assay. This is the first study to describe the complete
genome sequence of \bioname{V. tubiashii} infecting
ivory shell. The genetic characteristics, virulence
factors, and antimicrobial resistance of the
\bioname{V. tubiashii} strain FP17 were also
explored.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hameed:2022:SAI,
author = "Amna Hameed and Waqar Majeed and Muhammad Naveed and
Uzma Ramzan and Matteo Bordiga and Maryam Hameed and
Saud Ur Rehman and Naureen Rana",
title = "Success of Aquaculture Industry with New Insights of
Using Insects as Feed: a Review",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "395",
day = "17",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060395",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/395",
abstract = "Most of world's fish and seafood are produced by
aquaculture, which is one of the biggest contributors
to the world's food security. The substantial increase
in prices of conventional feed ingredients and the
over-exploitation of natural resources are some of the
biggest constraints to aquaculture production. To
overcome this stress, different approaches and
techniques are used, among which the use of
non-conventional feed ingredients in the aquaculture
sector is the most recent approach. Different
non-conventional feed ingredients such as plant-based
products, algae (both micro and macroalgae),
single-cell protein (bacteria and yeast), and insect
meal are currently used in aquaculture for sustainable
food production. Amongst all these novel ingredients,
insects have greater potential to replace fishmeal. The
existence of about 1.3 billion tons of food and
agriculture waste from the food chain supply poses a
serious environmental threat. Insects are tiny
creatures that can thrive on organic waste and thus can
convert the waste to wealth by the bioconversion and
nutritional upcycling of organic waste. Insects have
the potential to recover nutrients from waste
aquaculture products, and many fish species feed on
insects naturally. Therefore, employing insects in the
aquaculture sector to replace fishmeal is an
eco-friendly approach. The present review briefly
highlights emerging non-conventional feed ingredients,
with special attention given to insects. The current
review also focuses on the nutritional value of
insects, factors affecting the nutritional value of
insects, potential insects that can be employed in the
aquaculture sector, the physiological response of fish
when fed with insect meal, techno-functional properties
of insect meal, and emerging approaches for addressing
possible downsides of employing insect meal in fish
diets. Finally, it suggests avenues for further
research into these inventive fishmeal replacements.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Qin:2022:IIP,
author = "Gaixiao Qin and Jin Xu and Xiaohui Ai and Yibin Yang",
title = "Isolation, Identification, and Pathogenicity of
\bioname{Aeromonas veronii}, the Causal Agent of
Hemorrhagic Septicemia in Channel Catfish
(\bioname{Ictalurus punctatus}) in \geoname{China}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "394",
day = "17",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060394",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/394",
abstract = "Herein, we isolated the pathogenic strain ZZ051 from
hemorrhagic channel catfish (\bioname{Ictalurus
punctatus}). Physiological and biochemical
identification, 16S rRNA gene sequence analysis, and
MALDI-TOF-MS showed that the ZZ051 strain was
\bioname{Aeromonas veronii}. After artificial
infection, the diseased fish showed symptoms similar to
the natural disease, and the characteristics of the
bacteria reisolated from the tissues were the same as
those of the original infection, indicating that the
isolated strain ZZ051 was the pathogen responsible for
the channel catfish disease. The ZZ051 isolate was
highly sensitive to enrofloxacin but resistant to
florfenicol. This study provided a theoretical basis
for preventing and controlling hemorrhagic disease in
channel catfish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kennedy:2022:UFS,
author = "Emily K. C. Kennedy and David M. Janz",
title = "The Use of Fish Scale Hormone Concentrations in the
Assessment of Long-Term Stress and Associated Adverse
Effects on Reproductive Endocrinology",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "393",
day = "16",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060393",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/393",
abstract = "Investigation of the use of fish scales as a medium
for non-lethal biomonitoring has recently commenced.
Fish scales have been shown to incorporate cortisol
over longer periods of time than blood and thus provide
a promising means of assessing long-term stress in many
species of teleost fish. However, while cortisol is a
major mediator of the stress response in fishes,
downstream effects of chronic stress on reproduction
can involve gonadal steroids such as progesterone and
testosterone. The quantification of these additional
hormones alongside cortisol could therefore allow for
the assessment of both stress and consequential
reproductive alterations. To investigate these
concepts, we artificially elevated circulating
cortisol, progesterone, and testosterone in rainbow
trout (\bioname{Oncorhynchus mykiss}) using coconut oil
implants for three weeks. Following this we quantified
these three hormones as well as 11-ketotestosterone, a
potent androgen in teleost fishes. In all cases serum
samples reflected a significant increase in the
injected hormone confirming the efficacy of this
method; however, this did not result in significantly
elevated scale concentrations of the same hormone in
all cases. As the stress and reproductive axes are
closely integrated, these findings are likely a result
of interactions along the steroidogenic pathway
indicating that a further investigation of the
relationship between scale concentrations of these
hormones and actual physiological processes is
required. Nevertheless, the successful quantification
of both stress and gonadal steroid hormones within the
scale suggests that such measurements could provide a
novel and informative tool in the assessment of
long-term stress and the resulting effects on
reproductive endocrinology in teleost fishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lu:2022:CES,
author = "Min Lu and Zenghou Xing and Yurui Zhou and Youhou Xu
and Huijing Peng and Jie Zou and Solomon Felix Dan and
Zhicai She and Pengliang Wang and Jinfeng Liu and
Shaomin Qin and Jialin Yang and Peng Zhu",
title = "Cloning and Expression of \gene{Sox2} and \gene{Sox9}
in Embryonic and Gonadal Development of
\bioname{Lutraria sieboldii}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "392",
day = "16",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060392",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/392",
abstract = "The Sox family plays essential roles as transcription
factors in vertebrates; however, little is known about
the Sox family in Lutraria sieboldii. L. sieboldii are
pleasant to eat with a short growth cycle and have
become one of the best bottom-seeded enrichment species
in Guang Xi. In this study, \gene{Sox2} (named
\gene{LsSox2}) and \gene{Sox9} (named \gene{LsSox9})
from \bioname{L. sieboldii} were cloned, and their
expression patterns were analyzed. The length of the
\gene{LsSox 2} gene coding sequence was 1011 bp,
encoding 336 amino acids, and \gene{LsSox 9} was 1449
bp, encoding 482 amino acids. \gene{LsSox 2} had its
highest expression levels in the ovary, which were 356
times those in testis, whereas \gene{LsSox 9} presented
higher expression in testis, which was 6 times more
highly expressed than in the ovary. \gene{LsSox 2}
exhibited the highest expression during the morula
stage, which was 20 times that of the D-shaped larvae
or zygote. \gene{LsSox 9} exhibited two expression
peaks, one at the four-cell stage and the other at the
trochophore stage, while the lowest expression was in
the zygote. \gene{LsSox 9} was 73 times more highly
expressed in the four-cell stage than in the zygote
stage. During gonadal development, \gene{LsSox 2}
presented the highest expression in the mature ovary,
which was 756 times more highly expressed than in
mature testis. \gene{LsSox 9} presented higher
expression in testis at the emission stage which was 6
times more highly expressed than in the ovary. These
results indicate that \gene{LsSox 2} and \gene{LsSox 9}
may play important roles in embryonic and gonadal
development.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2022:CDM,
author = "Yang Liu and Yiying Jiang and Zhaobin Pei and Limin
Han and Hongrun Shao and Yang Jiang and Xiaomeng Jin
and Saihong Tan",
title = "Coordinated Development of the Marine Environment and
the Marine Fishery Economy in \geoname{China},
2011--2020",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "391",
day = "15",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060391",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/391",
abstract = "The marine environment is the material basis for the
survival and development of fishery resources, and
changes in the marine environment affect the fishery
economy. Therefore, against the background of
sustainability and environmental uncertainty, it is
important to investigate the development of the marine
environment and the marine fishery economy to improve
the quality of both. Taking the panel data for 11
coastal cities in China from 2011 to 2020, we use
several methods, including the entropy method, a
coupling harmonious degree model, and a Tobit model, to
measure the marine-environment quality and
marine-fishery-economy quality, their coordination, and
the factors affecting that coordination. We find that
(1) the marine-environment quality and marine-fishery
economy quality show a significant upward trend over
time, but they are spatially unbalanced, with obvious
interprovincial differences. (2) Coordination between
the marine-environment quality and
marine-fishery-economy quality has risen steadily, but
the level of coordination is still low, remaining at
the primary level in most areas. (3) The important
factors affecting coordination between the
marine-environment quality and marine-fishery-economy
quality include the strength of the marine fishery
industry, scale of the marine fishery economy,
production capacity of marine fisheries,
marine-environment quality, and quality of the marine
environment and its resources. In light of these
findings, we should increase the coordination between
the marine-environment quality and
marine-fishery-economy quality by upgrading the marine
fishery industry, modernizing marine fisheries, linking
the environmental governance of marine and land areas,
and strengthening the ecological construction of the
marine environment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Carral:2022:RDF,
author = "Jose M. Carral and Mar{\'\i}a S{\'a}ez-Royuela",
title = "Replacement of Dietary Fishmeal by Black Soldier Fly
Larvae (\bioname{Hermetia illucens}) Meal in Practical
Diets for Juvenile Tench (\bioname{Tinca tinca})",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "390",
day = "15",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060390",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/390",
abstract = "The development of specific diets for the juvenile
stage is a main target for culture intensification of
tench (\bioname{Tinca tinca}). Aquafeeds still rely
heavily on the use of fishmeal (FM) but concerns about
economic and ecological sustainability make the use of
alternative protein sources necessary. Insect meals are
considered a promising substitute to replace FM. In a
90-day experiment, 6 diets with different replacement
levels of FM by partially defatted black soldier fly
larvae meal (BSFLM): 0\%, 15\%, 30\%, 45\%, 60\% and
75\%, were tested on juvenile tench. Survival rates
were high (95.8--100\%) without differences between
treatments. Diet with 45\% FM replacement allowed for
best growth performance in terms of total length (TL =
66.4 mm) and weight (W = 4.19 g), without differences
with 60\% and 75\% of replacement. A cubic relationship
was evidenced between the level of FM replacement and
growth. From the regression equations, the estimated
optimal level of FM replacement was 47\% (356 g BSFLM
kg {$-$1} diet). Externally visible deformities were
always under 0.05\%. The whole-body lipid content of
the fish had a significantly negative linear regression
with BSFLM (r 2 = 0.80). The content of the essential
amino acids (EAA) arginine, leucine, lysine,
phenylalanine, methionine, and threonine in diets
decreased with dietary BSFLM inclusion. However, it did
not have a negative effect on growth performance,
suggesting that EAA requirements were covered. The
amount of essential amino acids in whole-body juveniles
was similar independently of the diet provided. The
results allow considering BSFLM as a sustainable
protein source for juvenile tench feeding.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Qu:2022:SAT,
author = "Yunfeng Qu and Ruiyang Liu",
title = "A Sustainable Approach towards Fisheries Management:
Incorporating the High-Seas Fisheries Issues into the
{BBNJ Agreement}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "389",
day = "14",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060389",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/389",
abstract = "The issue of high-seas fisheries is the main threat to
biodiversity in areas beyond national jurisdiction. The
BBNJ Agreement, which focuses on biodiversity in areas
beyond national jurisdiction, is under vigorous
discussion. Subject to the ``not undermine''
requirement and considerations of practical interests,
it is highly possible that the BBNJ Agreement may not
address the issue of fisheries on the high seas. The
objective of this paper is to analyze the relationship
between the high-seas fisheries issue and the BBNJ
Agreement for the purposes of the conservation of
marine biodiversity, the unity of the marine ecosystem,
and the consistency of regulations. It maintains that
from the perspective of protecting the oceans, enacting
legislation in areas beyond national jurisdiction, and
transforming marine management mode, the issue of
high-seas fisheries should be included in the BBNJ
Agreement. In the future, the BBNJ agreement needs to
clarify its scope of application, resolve overlapping
issues through general regulations and conflict rules,
clarify the methods and contents of international
cooperation, and establish international law
obligations for integrated ocean management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pizzul:2022:ENT,
author = "Elisabetta Pizzul",
title = "Editorial: New Trends in Freshwater Fishes",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "388",
day = "13",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060388",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/388",
abstract = "The issue of high-seas fisheries is the main threat to
biodiversity in areas beyond national jurisdiction. The
BBNJ Agreement, which focuses on biodiversity in areas
beyond national jurisdiction, is under vigorous
discussion. Subject to the ``not undermine''
requirement and considerations of practical interests,
it is highly possible that the BBNJ Agreement may not
address the issue of fisheries on the high seas. The
objective of this paper is to analyze the relationship
between the high-seas fisheries issue and the BBNJ
Agreement for the purposes of the conservation of
marine biodiversity, the unity of the marine ecosystem,
and the consistency of regulations. It maintains that
from the perspective of protecting the oceans, enacting
legislation in areas beyond national jurisdiction, and
transforming marine management mode, the issue of
high-seas fisheries should be included in the BBNJ
Agreement. In the future, the BBNJ agreement needs to
clarify its scope of application, resolve overlapping
issues through general regulations and conflict rules,
clarify the methods and contents of international
cooperation, and establish international law
obligations for integrated ocean management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Greco:2022:GEP,
author = "Samuele Greco and Anastasia Serena Gaetano and Gael
Furlanis and Francesca Capanni and Chiara Manfrin and
Piero Giulio Giulianini and Gianfranco Santovito and
Paolo Edomi and Alberto Pallavicini and Marco Gerdol",
title = "Gene Expression Profiling of \bioname{Trematomus
bernacchii} in Response to Thermal and Stabling
Stress",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "387",
day = "13",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060387",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/387",
abstract = "The Antarctic continent is one of the most pristine
environments on planet Earth, yet one of the most
fragile and susceptible to the effects of the ongoing
climate change. The overwhelming majority of the
components of Antarctic marine trophic chain are
stenotherm organisms, highly adapted to the extreme,
but extremely stable, freezing temperatures of the
Antarctic ocean, which have not changed significantly
during the past fifteen million years. Notothenioid
fishes are the most abundant representatives of
ichthyofauna at these latitudes, being ubiquitously
found in coastal areas across the entire continent.
While different Antarctic fish species have been
previously subjected to studies aimed at defining their
range of thermal tolerance, or at studying the response
to acute thermal stress, just a handful of authors have
investigated the effects of the exposure to a moderate
increase of temperature, falling within the expected
forecasts for the next few decades in some areas of the
Antarctica. Here, the emerald rockcod Trematomus
bernacchii was used as a model species to investigate
the effects of a 20-day long exposure to a +1.5
{$^\circ $} C increase in the brain, gills and skeletal
muscle, using a RNA-sequencing approach. In parallel,
the experimental design also allowed for assessing the
impact of stabling (including acclimation, the handling
of fishes and their confinement in tanks during the
experimental phase) on gene expression profiling. The
results of this study clearly identified the brain as
the most susceptible tissue to heat stress, with
evidence of a time-dependent response dominated by an
alteration of immune response, protein synthesis and
folding, and energy metabolism-related genes. While the
gills displayed smaller but still significant
alterations, the skeletal muscle was completely
unaffected by the experimental conditions. The stabling
conditions also had an important impact on gene
expression profiles in the brain, suggesting the
presence of significant alterations of the fish nervous
system, possibly due to the confinement to tanks with
limited water volume and of the restricted possibility
of movement. Besides providing novel insights in the
molecular mechanisms underlying thermal stress in
notothenioids, these findings suggest that more
attention should be dedicated to an improved design of
the experiments carried out on Antarctic organism, due
to their extreme susceptibility to the slightest
environmental alterations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lin:2022:APP,
author = "Pei-Hui Lin and Sai-Wei Chen and Zhi-Hong Wen and
Shao-Yang Hu",
title = "Administration of the Potential Probiotic
\bioname{Paenibacillus ehimensis} {NPUST1} Enhances
Expression of Indicator Genes Associated with Nutrient
Metabolism, Growth and Innate Immunity against
\bioname{Aeromonas hydrophila} and
\bioname{Streptococcus indie} Infections in Zebrafish
(\bioname{Danio rerio})",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "386",
day = "12",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060386",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/386",
abstract = "Probiotics as antibiotic alternatives for preventing
and controlling infectious diseases are a relatively
eco-friendly strategy in aquaculture. The
bacteriocin-producing probiotic \bioname{Paenibacillus
ehimensis} NPUST1 was isolated from tilapia culture
pond water in our previous study. The present study
demonstrated that \bioname{P. ehimensis} NPUST1
produced hydrolytic enzymes, including protease,
amylase, cellulase, xylanase and lipase. The effects of
\bioname{P. ehimensis} NPUST1 on zebrafish nutrient
metabolism, growth performance and innate immunity were
evaluated by measuring the expression of indicator
genes in zebrafish after feeding \bioname{P. ehimensis}
NPUST1 at doses of 10 6 and 10 7 CFU/g for 8 weeks.
There was an obvious increase in the hepatic mRNA
expression of carbohydrate metabolism-related genes,
including glucokinase, hexokinase 1,
glucose-6-phosphatase, and pyruvate kinase, and
growth-related genes, including the growth hormone
receptor and insulin-like growth factor-1. The
expression of the innate immune-related genes including
interleukin (IL)-$ 1 \beta $, IL-6, IL-15, tumor
necrosis factor-$ \alpha $, toll-like receptor (TLR)-1,
TLR-4, complement component C3b and lysozyme were
significantly increased in \bioname{P. ehimensis}
NPUST1-supplemented fish. A significant reduction in
cumulative mortality was exhibited in \bioname{P.
ehimensis} NPUST1-supplemented fish after infection
with \bioname{Aeromonas hydrophila} and
\bioname{Streptococcus iniae}. In conclusion, our data
suggested that \bioname{P. ehimensis} NPUST1
supplementation in feed could potentially improve
nutrient metabolism and growth in addition to enhancing
innate immunity and disease resistance against
\bioname{A. hydrophila} and \bioname{S. iniae} in
zebrafish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luo:2022:MEI,
author = "Wei Luo and Chen Li and Kang Wu and Songming Zhu and
Zhangying Ye and Jianping Li",
title = "A Method for Estimating the Injection Position of
Turbot (\bioname{Scophthalmus maximus}) Using Semantic
Segmentation",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "385",
day = "11",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060385",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/385",
abstract = "Fish vaccination plays a vital role in the prevention
of fish diseases. Inappropriate injection positions
will cause a low immunization rate and even death.
Currently, traditional visual algorithms have poor
robustness and low accuracy due to the specificity of
the placement of turbot fins in the application of
automatic vaccination machines. To address this
problem, we propose a new method for estimating the
injection position of the turbot based on semantic
segmentation. Many semantic segmentation networks were
used to extract the background, fish body, pectoral
fin, and caudal fin. In the subsequent step, the
segmentations obtained from the best network were used
for calculating body length (BL) and body width (BW).
These parameters were employed for estimating the
injection position. The proposed Atten-Deeplabv3+
achieved the best segmentation results for intersection
over union (IoU) on the test set, with 99.3, 96.5,
85.8, and 91.7 percent for background, fish body,
pectoral fin, and caudal fin, respectively. On this
basis, the estimation error of the injection position
was 0.2 mm--4.4 mm, which is almost within the
allowable injection area. In conclusion, the devised
method was able to correctly differentiate the fish
body from the background and fins, meaning that the
extracted area could be successfully used for the
estimation of injection position.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barua:2022:ALB,
author = "Suman Barua and Qun Liu and Mohammed Shahidul Alam and
Petra Schneider and Mohammad Mojibul Hoque Mozumder",
title = "Application of Length-Based Assessment Methods to
Elucidate Biological Reference Points of Black Pomfret
Stock in the \geoname{Bay of Bengal, Bangladesh}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "384",
day = "11",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060384",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/384",
abstract = "The black pomfret (\bioname{Parastromateus niger}) is
one of the high-income-generating carangid fish species
and the only known member of its genus. This study aims
to identify existing gaps in stock status and
population demography of this species and provide
viable management recommendations to enhance the
sustainability of this fishery. Therefore, three
methodological approaches have been used in this study;
TropFishR to present the current exploitation status,
the length-based spawning potential ratio (LB-SPR) to
quantify stock spawning biomass, and Froese's
sustainability indicators (LBI) to establish a basic
standard for sustainable management of the fishery. In
the length--weight relationship of black pomfret, an
allometric growth pattern ($ b = 2.19$) was found. The
VBGF life history parameters for black pomfret were L
{\infty} = 55.25 cm, K = 0.54 year {$-$1}, and based on
LCCC analysis, the total mortality ($ Z = 1.61$ year -
1), natural mortality ($ M = 0.69$ year - 1) and
fishing mortality ($ F = 0.91$ year -1) are calculated.
The result reveals that this fishery is currently
suffering from overexploitation and the stock's
spawning biomass (SPR = 13\%) is below the limit
reference point because most of the catch (82\%) was
found to be under the maturity level. Based on the
results, this study recommended strictly maintaining
the mesh size of the net to ensure not to catch
immature fish with a length smaller than 30.63 cm,
recommended the length to catch be between 29 and 35
cm, and reducing fishing pressure by one-third to
ensure the sustainability of the black pomfret
fishery.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rodriguez-Rey:2022:DDS,
author = "Marta Rodr{\'\i}guez-Rey and Ga{\"e}l Grenouillet",
title = "Disentangling the Drivers of the Sampling Bias of
Freshwater Fish across \geoname{Europe}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "383",
day = "10",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060383",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/383",
abstract = "The Wallacean shortfall refers to the knowledge gap in
biodiversity distributions. There is still limited
knowledge for freshwater fish species despite the
importance of focusing conservation efforts towards
this group due to their alarming extinction risk and
the increasing human pressure on freshwater ecosystems.
Here, we addressed the Wallacean shortfall for
freshwater fish faunas across Europe by using the
completeness indicator derived from species
accumulation curves to quantify the fish sampling
efforts. The multiple potential drivers of completeness
that were previously related to the sampling efforts
for other species (i.e., population density, nature
reserves, or distance to cities) were tested using a 10
$ \times $ 10 km 2 grid resolution, as well as
environmental (e.g., climatic) factors. Our results
suggested that although there was an overall spatial
pattern at the European level, the completeness was
highly country-dependent. Accessibility parameters
explained the sampling efforts, as for other taxa.
Likewise, climate factors were related to survey
completeness, possibly pointing to the river conditions
required for fish sampling. The survey effort map we
provide can be used to optimize future sampling, aiming
at filling the data gaps in undersampled regions like
the eastern European countries, as well as to account
for the current bias in any ecological modeling using
such data, with important implications for conservation
and management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ren:2022:CFA,
author = "Xiaoliang Ren and Shulun Jiang and Long Ren and Yidong
Liang and Di'an Fang and Dongpo Xu",
title = "Changes in Fish Assemblage Structure after Pen Culture
Removal in \geoname{Gehu Lake, China}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "382",
day = "09",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060382",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/382",
abstract = "The removal of the net enclosure has been used as a
lake management strategy in various regions of China as
ecological development is given more attention.
Nevertheless, little is known about the substantive
impact of this measure on fish communities in inland
lakes. To this end, the fish community composition and
structural features after the removal of the net
enclosure in Gehu Lake were explored and evaluated in
this study from 2021 to 2022 and compared to the
investigation before the net enclosure removal from
2017 to 2018. Belonging to 7 orders, 10 families, and
46 species, a total of 17,151 fish were collected, with
pelagic, sedentary, and omnivorous species dominating.
In comparison, the number of species increased by 10
after removal, and fish alpha diversity increased. The
Index of Relative Importance (IRI) revealed that the
composition of dominating species remained constant,
including Coilia nasus, Hypophthalmichthys molitrix,
and Hypophthalmichthys nobilis; Parabramis pekinensis,
Megalobrama amblycephal, and Culter mongolicus were
upgraded from common species to general species. Culter
alburnus, Hemiculter bleekeri, and Pseudobrama simoni
were downgraded from general species to common species.
Elopichthys bambusa had become a common species (IRI =
109.35), which was not discovered before removal.
According to the hierarchical clustering (HC) and
non-metric multidimensional sequencing (NMDS), the fish
community of the northern reserve was highly
aggregated. As the Abundance Biomass Comparison (ABC)
curve and biodiversity index indicated, the fish
community structure of the whole lake was in a state of
moderate anthropogenic disturbance with reduced
stability, while that of the northern reserve was in a
state of light anthropogenic disturbance with greater
stability. The number of fish species increased in this
survey compared to the period before removal, species
and dominant species composition altered dramatically,
and total lake stability declined. This study
demonstrates that the fish diversity in Gehu Lake
increased after the removal of the net enclosure.
Meanwhile, the stability of the fish community
structure was decreased temporarily. Lake restoration
is a long-term process, and the underlying impact of
the removal of the net enclosure still requires
continuous monitoring and further studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zaccone:2022:INI,
author = "Giacomo Zaccone",
title = "Immunity and Neuroimmune Interactions at the Mucosal
Barriers in Fish",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "381",
day = "08",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060381",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/381",
abstract = "The removal of the net enclosure has been used as a
lake management strategy in various regions of China as
ecological development is given more attention.
Nevertheless, little is known about the substantive
impact of this measure on fish communities in inland
lakes. To this end, the fish community composition and
structural features after the removal of the net
enclosure in Gehu Lake were explored and evaluated in
this study from 2021 to 2022 and compared to the
investigation before the net enclosure removal from
2017 to 2018. Belonging to 7 orders, 10 families, and
46 species, a total of 17,151 fish were collected, with
pelagic, sedentary, and omnivorous species dominating.
In comparison, the number of species increased by 10
after removal, and fish alpha diversity increased. The
Index of Relative Importance (IRI) revealed that the
composition of dominating species remained constant,
including Coilia nasus, Hypophthalmichthys molitrix,
and Hypophthalmichthys nobilis; Parabramis pekinensis,
Megalobrama amblycephal, and Culter mongolicus were
upgraded from common species to general species. Culter
alburnus, Hemiculter bleekeri, and Pseudobrama simoni
were downgraded from general species to common species.
Elopichthys bambusa had become a common species (IRI =
109.35), which was not discovered before removal.
According to the hierarchical clustering (HC) and
non-metric multidimensional sequencing (NMDS), the fish
community of the northern reserve was highly
aggregated. As the Abundance Biomass Comparison (ABC)
curve and biodiversity index indicated, the fish
community structure of the whole lake was in a state of
moderate anthropogenic disturbance with reduced
stability, while that of the northern reserve was in a
state of light anthropogenic disturbance with greater
stability. The number of fish species increased in this
survey compared to the period before removal, species
and dominant species composition altered dramatically,
and total lake stability declined. This study
demonstrates that the fish diversity in Gehu Lake
increased after the removal of the net enclosure.
Meanwhile, the stability of the fish community
structure was decreased temporarily. Lake restoration
is a long-term process, and the underlying impact of
the removal of the net enclosure still requires
continuous monitoring and further studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bakit:2022:DEE,
author = "Jos{\'e} Bakit and Gonzalo {\'A}lvarez and Patricio A.
D{\'\i}az and Eduardo Uribe and Rodrigo Sfeir and
Sebastian Villasante and Tomas Gabriel Bas and
Germ{\'a}n Lira and Hern{\'a}n P{\'e}rez and Andr{\'e}s
Hurtado and Ra{\'u}l Gonz{\'a}lez-{\'A}valos and Jose
Castillo-Venenciano",
title = "Disentangling Environmental, Economic, and
Technological Factors Driving Scallop
(\bioname{Argopecten purpuratus}) Aquaculture in
\geoname{Chile}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "380",
day = "08",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060380",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/380",
abstract = "The boom-and-bust trajectory of the Argopecten
purpuratus industry in Chile shows the progression from
resource extraction (fishing) to production
(aquaculture). This paper analyses the effects of
environmental, economic, and scientific--technological
factors. The influence of each factor on scallop
production in Chile was reviewed for the period between
the 1980s and 2020. The evaluation of the effects
allows the visualisation of the industry's productive
evolution and reveals the current challenges. The
occurrence of abrupt environmental disturbances,
commercialisation under imperfect market
configurations, and public and private efforts in
scientific and technological advances have acted
favourably on scallop production. However, an industry
mainly focused on prices and high production volumes
did not devote much effort to develop low-cost
climate-resilient technologies. Today, economic
challenges must be addressed by helping to reduce
production costs and add economic value to products and
by-products. Our results show that the industry must
focus on low-cost technologies, the use of renewable
energy, and the circularity of its processes. The
environment ensures the capture of natural seeds and
their adaptation to climate change. These challenges
must not lose sight of the emerging effects of the
COVID-19 pandemic.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ding:2022:GMN,
author = "Liyun Ding and Yanping Zhang and Jiacheng Chen and
Wenjing Chen and Shouqi Xie and Qingtang Chen",
title = "Growth, Muscle Nutrition Composition, and Digestive
Enzyme Activities of the Juvenile and Adult
\bioname{Siniperca chuatsi} Fed on Live Baits and a
Formulated Diet",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "379",
day = "08",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060379",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/379",
abstract = "The present study aimed to evaluate the feasibility of
a formulated diet to replace live baits as feed for
Siniperca chuatsi. A 2 $ \times $ 2 factorial design
with three replicates was used to investigate the
effects of conventional live baits (LB) and a
formulated diet (FD) on the growth, muscle nutrition
composition, and digestive enzyme activities of S.
chuatsi at two growth stages. The results showed that
the feed conversion ratio (FCR) in the FD group was
significantly lower than that in the LB group. The
final body weight (FBW) and weight gain (WG) were not
significantly different between the two feeding modes.
However, at the juvenile stage, the specific growth
rate (SGR) in the FD group was significantly lower than
that in the LB group. Muscle moisture, crude protein,
and crude lipid contents were not influenced by dietary
treatments. There were no significant differences in
{\SGMLsum}EAA, {\SGMLsum}NEAA, and {\SGMLsum}AA
compositions of the juvenile and adult stages fed the
two kinds of diets. At the juvenile stage, histidine
(His) content in the FD group was significantly higher
than that in the LB group; at the adult stage, cysteine
(Cys) content in the FD group was significantly higher
than that in the LB group; at both growth stages, Met
content in the FD group was significantly lower than
that in the LB group. The FD group showed higher levels
of {\SGMLsum}SFA, {\SGMLsum}HUFA, {\SGMLsum}n-3PUFA,
n-3/n-6, EPA, and DHA than the LB group did, whilst the
opposite occurred in the MUFA levels. In addition, fish
fed with LB had significantly higher values of pepsin
and intestinal trypsin activities in the juvenile fish
compared to the FD group, but the activities were not
significantly different between the two feeding modes
at the adult stage. The activities of stomach and
intestine amylase in the FD group were significantly
higher than those in the LB group. Overall, these
results showed that under long-term feeding conditions,
S. chuatsi fed with the artificial diet had no
significant difference in muscle crude protein, crude
lipid, amino acid composition, and digestive capacity,
but showed superiority in the composition of fatty
acids such as EPA and DHA levels compared to the live
baits group. Therefore, the artificially formulated
diet might be a more appropriate feeding approach for
sustainable development of the S. chuatsi industry.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2022:EDP,
author = "Ronghua Wang and Chaobo Lei and Zhenyu Li and Yanju
Lei and Congqiang Luo and Liye Shao and Chunhong Huang
and Pinhong Yang",
title = "Effects of a Diet of \bioname{Phragmites australis}
instead of \bioname{Triticum aestivum} {L.} on Immune
Performance and Liver Tissue Structure of
\bioname{Ctenopharyngodon idellus}",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "378",
day = "08",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060378",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/378",
abstract = "This experiment aimed to study the effects on liver
tissue structure and immune performance of grass carp
Ctenopharyngodon idellus when the common reed
Phragmites australis is in its diet, instead of wheat
Triticum aestivum L. Seventy-two healthy grass carps
(145.52 {\pm} 2.56 g) were randomly divided into three
groups according to their body weight. Fishes in each
group were fed an essential diet with 0\% (control
group), 50\% and 100\% (test group) common reed,
instead of wheat, respectively. After feeding for 41
days, the changes of serum biochemical indices, liver
tissue structure and immune related indices of grass
carp were detected. The results showed that, compared
with the control group, the activities of serum alanine
transaminase (ALT) and aspartate transaminase (AST) in
the substitution groups were significantly increased ($
p < 0.05$), but still at a normal level. The contents
of total protein, albumin and globulin did not change
significantly ($ p > 0.05$). Compared with the control
group, the liver cells of grass carp in the
substitution groups had clear boundaries, tight
arrangement and less vacuolation. The contents of serum
interleukin-1 (IL-1) and complement 3 (C3) in the 100\%
substitution group were significantly higher than those
in the control group ($ p < 0.05$), and the contents of
liver IL-1 and total complement (CH50) in the 100\%
substitution group were significantly higher than those
in the control group ($ p < 0.05$). The contents of
IL-1, C3 in serum and IL-1, CH50 in liver in the 50\%
substitution group were significantly higher than those
in the control group ($ p < 0.05$). The mRNA relative
expression levels of C3, IL-1, MHC-I and interferon
(IFN) in the head-kidney, kidney, liver and spleen of
grass carp were significantly affected by feeding the
grass carp with different common reed substitution
ratios ($ p < 0.05$). In summary, common reed, instead
of wheat, in feed can improve the liver tissue
structure, and increase the non-specific immune
response level, of grass carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xiao:2022:MCD,
author = "Yangbo Xiao and Rong Huang and Shenping Cao and Dafang
Zhao and Zhuangwen Mao and Chuchu Xiao and Zhehua Xu
and Xiaomei Zhou and Xinran Zhang and Yu Zhang and
Jianzhou Tang and Junyan Jin and Yaoguo Li and Jun Zou
and Zhen Liu",
title = "Molecular Characterization and Dietary Regulation of
Glutaminase 1 (\gene{gls1}) in Triploid Crucian Carp
(\bioname{Carassius auratus})",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "377",
day = "07",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060377",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/377",
abstract = "Kidney-type glutaminase, encoded by the gls1 gene,
plays a critical role in glutamate production and
improvement of meat flavor. In this study, a gls1 gene
encoding 595 amino acids was cloned from triploid
crucian carp (\bioname{Carassius auratus}) (TCC) and
showed a high similarity with the gls1 gene found in
Cyprinus carpio, Sinocyclocheilus rhinocerous and
Puntigrus tetrazona. Comparing the abundance of gls1 in
different tissues, we found its expression level in the
brain and liver were significantly higher than that in
heart, gut, kidney, spleen and muscle. gls1 expression
in the brain reached the highest value. In addition,
the expression levels of gls1 also appeared different
in diurnal variation, with the highest expression seen
at 9:00, while it was low at 3:00, 6:00, 15:00 and
24:00. Furthermore, dietary regulation of gls1
expression was investigated in our study. In each
feeding trial, each diet was randomly assigned to
triplicate tanks. Fish were fed one of the tested diets
up to satiation twice daily. The results showed that
gls1 expression increased in 32\% protein group and
decreased in 35--41\% protein group. The results of
different protein source experiments showed that the
expression of gls1 gene in the mixed protein group (the
control group) was significantly higher than that in
the fish meal and soybean meal groups. Glutamate
treatment revealed that appropriate concentrations
(0.10 mg/mL in vivo and 2.00\% in vitro) of glutamate
remarkably improved the expression of gls1. Besides,
diets supplemented with 0.80--1.60\% lysine-glutamate
dipeptide exhibited a down regulatory impact on gls1
expression. In conclusion, this study demonstrated that
the expression of gls1 in TCC was increased by 32\%
protein diet, mixed protein source diet and diet with
2.00\% glutamate concentration, while decreased by
0.80--1.60\% lysine-glutamate dipeptide. The findings
of this study provide a reference for the regulation of
gls1 and have a potential application in the
optimization of dietary formula in aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Silkin:2022:ERA,
author = "Yuriy A. Silkin and Mikhail Yu. Silkin and Sergey M.
Korotkov and Elizaveta N. Silkina and Alla Silkina",
title = "Erythrocyte Respiratory Activity of the Mitochondrial
Complex of the Black Sea Thornback Ray (\bioname{Raja
clavata} {L.}) under the Influence of Certain
Activators and Inhibitors In Vitro",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "376",
day = "07",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060376",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/376",
abstract = "Global warming implies the risk of a changing oxygen
regime in the seas and oceans of our planet. The
mitochondrial complex of nuclear erythrocytes of
cartilaginous fish, as the energy basis of blood cells,
has repeatedly encountered such climatic fluctuations
throughout their evolutionary history. In this regard,
the features of the adaptive strategy of the
erythrocyte mitochondrial complex in the thornback ray
(\bioname{Raja clavata} {L.}) are of interest from the
evolutionary and ecological points of view. The rate of
oxygen consumption in resuspended (Ht = 25--30\%)
erythrocytes taken from the Black Sea thornback ray in
saline was studied by the polarographic method. A high
``basal'' rate of respiration in the erythrocytes of
the thornback ray was shown, which ranged from 10.5 to
21.6 pmol O 2 min {$-$1} {\cdot}10 6 cells. The
addition of substrates of the mitochondrial respiration
activators glutamate, maleate, and succinate to the
erythrocyte suspension caused a 2--6-fold increase in
the respiratory activity of thornback ray erythrocytes.
In cases where the rate of respiration of erythrocytes
was high, protonophore--dinitrophenol caused an
inhibition of the activity of mitochondrial
respiration. At low respiration rates of erythrocytes,
its effect was opposite and caused a stimulation of
mitochondrial respiration. Oligomycin caused a
significant inhibition of the respiratory activity of
the red blood cell suspension of the thornback ray.
This suppression of cell respiration was enhanced under
conditions of exposure to the permeabilization of
erythrocytes with digitonin. This can be recommended as
one of the ways to block the respiratory activity of
erythrocytes in cartilaginous fish. Another way of
effectively blocking the respiration of the
mitochondrial complex of the thornback ray's
erythrocytes was the effect of the blockers rotenone
and sodium azide. The peculiarity of the mitochondria
of the erythrocytes of the thornback ray was the
absence of the complete inhibition of respiration by
sodium azide (NaN 3), which is characteristic of the
mitochondria of other fish species. Our data on the
activation of the ``respiration'' of erythrocytes in
fish indicate that the potential capabilities of
cold-blooded and warm-blooded vertebrates have rather
similar characteristics. This may indicate the initial
``laying'' of the architecture of the inner membrane to
support the energy potential of the mitochondria of the
cell.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Reyes-Mero:2022:YAR,
author = "Byron Manuel Reyes-Mero and Ana Mar{\'\i}a
Santana-Pi{\~n}eros and Leonela Griselda
Mu{\~n}oz-Chumo and Yanis Cruz-Quintana and Enric
Gisbert",
title = "Yolk Absorption Rate and Mouth Development in Larvae
of \bioname{Dormitator latifrons} ({Perciformes:
Eleotridae})",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "375",
day = "07",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060375",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/375",
abstract = "Fish larvae suffer high starvation mortality during
the transition from yolk absorption to exogenous
feeding, and the size of the developing buccal
structures limits the food they can consume.
Determining the suitable timing and size of live or
inert foods could decrease this mortality. We described
mouth development and determined the yolk absorption
rate and point-of-no-return (PNR) of Dormitator
latifrons larvae. One male and one female specimen were
induced to spawn using salmon GnRHa implants, and 45 of
their larvae were sedated and observed under a
microscope every 24 h to measure total length, standard
length, yolk sac length, yolk sac width, oil globule
length, oil globule width, width of the oesophagus, and
length of the upper and lower jaw longitudinal. The
growth model, maximum mouth opening, daily survival,
and starvation period were determined. The larval
growth was fastest during the first 24 h {post$-$
hatching} (HPH) at 28 {\pm} 1 {$^\circ $}C with an
average increase of 625.42 $ \mu $ m in total length
and 573.51 $ \mu $ m in standard length. The highest
percentage of yolk absorption (52\%) occurred within 24
HPH and at 96 HPH the yolk sac was completely
reabsorbed. The PNR was reached at 156.41 HPH ($ p <
0.05$). At 96 HPH, the upper and lower jaw were
distinguishable by mouth movements. Our results suggest
that the larvae of {$ D. l a t i f r o n s$} should be
fed at 96 HPH with prey measuring 50--65 $ \mu $ m.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2022:EWT,
author = "Xingbing Wu and Xuemei Li and Yongjiu Zhu and Jinling
Gong and Tingbing Zhu and Jiajia Ni and Deguo Yang",
title = "Effects of Water Temperature on the Growth,
Antioxidant Capacity, and Gut Microbiota of
\bioname{Percocypris pingi} Juveniles",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "374",
day = "06",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060374",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/374",
abstract = "It is necessary to determine the optimal temperature
for Percocypris pingi growth in recirculating
aquaculture systems. To describe the effects of
temperature, we evaluated the growth, antioxidant
enzyme activity, and gut microbiota structure of
\bioname{P. pingi} at different temperatures, including
14, 18, 22, and 26 {$^\circ $}C. Results showed that
increases in body weight of individuals of the groups
subjected to 18 and 22 {$^\circ $}C temperatures were
considerably higher than those in the groups subjected
to temperatures of 14 and 26 {$^\circ $}C between 20
and 60 d after the experiment started. Acid phosphatase
activity in the liver and kidneys of \bioname{P. pingi}
did not differ significantly among the various
temperature groups ($ p > 0.05$). A gradual restoration
of the alkaline phosphatase and superoxide dismutase
activities to variations in the surrounding temperature
was observed in the liver and kidney of P. pingi.
Interestingly, the water temperature did not affect the
{\alpha}-diversity or composition of the gut microbiota
of P. pingi. In conclusion, water temperatures between
14 and 26 {$^\circ $}C significantly impacted the
growth of P. pingi ($ p < 0.05$) but not the liver and
kidney antioxidant capacity or the gut microbiota
within 60 d.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dimitriadi:2022:EET,
author = "Anastasia Dimitriadi and George Koumoundouros",
title = "Elevated Embryonic Temperature Has Persistent Adverse
Effects on Zebrafish Swimming Capacity",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "373",
day = "06",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060373",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/373",
abstract = "In recent years, global warming of anthropogenic
origin and its impacts on biodiversity have
increasingly gained public awareness. Here, we
demonstrate that embryonic temperature can have
persistent and crucial effects on zebrafish swimming
capacity and cardiac shape. Three different embryonic
temperature treatments (T E = 24, 28 or 32 {$^\circ
$}C) were applied to zebrafish embryos until hatching.
Fish were then raised in common conditions (28 {$^\circ
$}C) until adulthood. Ventricle roundness was found to
increase significantly with a rise in T E in juvenile
(10\% increase) and male (8\% increase), but not female
fish. T E and sex significantly affected zebrafish
swimming performance. Juveniles, males and females
raised in cold (24 {$^\circ $}C) presented
significantly greater swimming capacity than those
raised at 28 and 32 {$^\circ $}C T E. Our results
represent a direct link between the physical capacity
of adult fish and embryonic temperature fluctuations
that add to the emerging rationale of the potential
climate change scenarios on wild fish populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fu:2022:CTH,
author = "Qianni Fu and Jinxia Liu and Tianjiao Ren and Zining
Zhang and Zihang Ma and Zhenyu Lan and Yitao Duan and
Ziwei Liang and Boyu Chen and Yan Zhang and Peng Zhu
and Yongyan Liao",
title = "Cloning of Two {HSP} Genes of \bioname{Eriocheir
hepuensis} and Their Expression under \bioname{Vibrio
parahaemolyticus} Stress",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "372",
day = "05",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060372",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/372",
abstract = "Heat shock proteins (HSPs) are molecular chaperone
proteins that can help maintain cellular protein
homeostasis, assist in correcting the folding of
cellular proteins, and protect organisms from stress
when the body is under stress conditions such as
temperature changes or bacterial infections. In this
study, the HSP 10 and HSP 40 genes of Eriocheir
hepuensis were cloned and named Eh-HSP10 and Eh-HSP40.
The results show that the coding sequence length of the
HSP 10 and HSP 40 genes of E. hepuensis was 309 bp and
1191 bp, encoding 102 and 396 amino acids,
respectively. The results of protein domain prediction
show that Eh-HSP10 has a Cpn10 domain. The Eh-HSP40
protein contains a DnaJ domain, which is characteristic
of the HSP 40 gene family. The results of qRT-PCR show
that the Eh-HSP 10 and Eh-HSP 40 genes were expressed
in different normal tissues, with the highest
expression in the heart. Under Vibrio parahaemolyticus
stress, the Eh-HSP 10 genes peaked at 6 h, and the
Eh-HSP40 peaked at 9 h in the hepatopancreas. In the
gill, Eh-HSP10 showed a double peak at 24 and 48 h, and
the expression of Eh-HSP40 was time-dependent. In the
heart, the expression of Eh-HSP10 increased first and
then decreased, whereas Eh-HSP40 peaked at 48 h. The
results indicate that the Eh-HSP10 and Eh-HSP40
proteins may play a role in protecting E. hepuensis
under V. parahaemolyticus infection and that they may
be involved in the innate immune response of E.
hepuensis against bacteria.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gu:2022:EEM,
author = "Lingling Gu and Hui Zhang and Guangpeng Feng and Yong
Liu and Zhiqiang Han and Feng Zhao and Qing Ye and
Wangjiao Hu and Chao Song",
title = "Evaluation of the Effectiveness of Marking Juvenile
\bioname{Takifugu obscurus} Otoliths with Strontium",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "371",
day = "05",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060371",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/371",
abstract = "Strontium marking technology is commonly used for
extensive marking in stock enhancement and releasing
activities. In order to explore the feasibility of this
technology for juvenile Takifugu obscurus, five
different concentrations of strontium (0, 12, 18, 36,
and 72 mg/L) were set up by strontium chloride
hexahydrate (SrCl 2 {\cdot}6H 2 O) and T. obscurus were
immersed for 7 days. Then, T. obscurus were reared in
non-additive water for 25 days. The results showed that
the survival rate of all groups was 100\%, except the
72 mg/L group, after 7 days of immersion. Moreover, the
growths of all the marked groups were better than that
of the control group. There was no significant
difference between the control and marked groups,
except for the 18 mg/L group, indicating that the
appropriate concentration of strontium might have
positive effect for T. obscurus. The strontium to
calcium (Sr / Ca) ratios of otoliths in the marked
groups increased with increasing concentration and
time, which were higher than the baseline,
respectively. Then, the Sr / Ca ratios returned to the
original level, but the peak value was still retained,
suggesting that the success rate of the strontium
marking reached 100\%. Notably, the residual strontium
content of 18 mg/L group was insufficient for causing
negative effects for T. obscurus after 25 days.
Therefore, in consideration of the growth, survival,
and effect of strontium marking on otolith, 18 mg/L is
an appropriate concentration for strontium marking of
juvenile T. obscurus. This study confirmed the
feasibility of strontium marking for T. obscurus and
provided a new approach to rationally and
scientifically evaluate the stock enhancement and
releasing efficiency of T. obscurus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Seo:2022:DID,
author = "Haneul Seo and Andre Ditya Maulana Lubis and Tae-Jin
Choi and Tae-Sung Jung and Taek-Kyun Lee and Sukchan
Lee",
title = "Development of an Immunoassay Detection System for Koi
Herpesvirus Using Recombinant Single-Chain Variable
Fragments",
journal = j-FISHES,
volume = "7",
number = "6",
pages = "370",
day = "02",
month = dec,
year = "2022",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes7060370",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:32 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/7/6/370",
abstract = "Koi herpesvirus (KHV) is a highly contagious virus
that causes high mortality in koi and common carp,
leading to a reduction in production worldwide. Recent
diagnostic tests based on molecular methods alone
(nucleic acid amplification) and indirect immunoassay
methods (antibody detection) can be confirmed over KHV
infections or prior exposure and latent infections.
Unfortunately, there is no established method to detect
KHV virus particles, especially when virus titers are
low. Therefore, we propose an alternative, direct
immunoassay method for viral detection using a
single-chain variable fragment (scFv), a specific
region of IgG antibodies that binds specifically to KHV
particles. The results of functional analyses indicated
that four putative scFv candidates, C5, F8, F6, and E4,
were specific to KHV, but only F6 and C5 had a high
binding affinity. The binding characteristics were
confirmed by indirect competitive and sandwich
enzyme-linked immunosorbent assays, which indicated
that F6 and C5 have a broad penetration area to the
binding region and share a similar epitope with
commercial KHV monoclonal antibodies. These
characteristics were further confirmed by their
interactions with purified KHV coat protein by indirect
ELISA and Western blot analyses. In conclusion, the F6
and C5 scFvs have adequate binding affinity to KHV
particles to permit their use in immunoassays.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sun:2023:RAO,
author = "Shuang-Shuang Sun and Shi-Wei Ma and Jun Li and Qin
Zhang and Guang-Zhou Zhou",
title = "Review on the Antiviral Organic Agents against Fish
Rhabdoviruses",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "57",
day = "16",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010057",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/57",
abstract = "Fish rhabdoviruses are harmful single-stranded RNA
viruses with high mortality rates which cause
considerable economic losses in aquaculture. It is
imperative to explore and develop new antiviral
compounds against them. In recent years, in addition to
inorganic antiviral substances, more than 50 different
organic compounds have been confirmed to be effective
in the prevention and treatment of rhabdovirus
infection and its dissemination in fish. The main types
of extracts or agents and their trial designs are here
considered for review. This review reveals the reported
antiviral activities of extracts from organisms,
proteins, lipids, polysaccharides, nucleic acids,
coumarin derivatives, arctigenin derivatives, and other
antiviral organic molecules against fish rhabdoviruses,
respectively. Additionally, their antiviral mechanisms
of action include direct virucidal effects, inhibiting
virus-induced host cell apoptosis, the blocking of the
viral replication cycle, affecting gene expression and
innate antiviral immune responses, and so on. This
review also gives perspectives on how to
comprehensively explore the potential applications of
the candidate molecules, which lay the foundation for
the future development of new compounds or strategies
for the prevention and control of fish rhabdoviruses in
aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:AFH,
author = "Siqing Xu and Peng Sun and Chi Zhang and Jianchao Li
and Xiaoyu Xi and Shuyang Ma and Wenchao Zhang and
Yongjun Tian",
title = "Age and Feeding Habits of Caml Grenadier
\bioname{Macrourus caml} in \geoname{Cosmonauts Sea}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "56",
day = "16",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010056",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/56",
abstract = "Macrourus caml is a main by-catch in the Southern
Ocean fishery and a main prey species of Antarctic
toothfish Dissostichus mawsoni; it plays an important
role in the Southern Ocean ecosystem. In this study,
age estimation and stomach content analysis were
conducted by using samples collected from the
Cosmonauts Sea in 2021. The main objectives of this
study were to estimate the age and diet of grenadier M.
caml and explore the feeding habits of M. caml.
Morphological analysis and molecular identification
were conducted to determine the diet of M. caml in this
study. Stomach content analysis showed that M. caml
mainly fed on Malacostraca, Sagittoidea, Cnidaria and
Algae, with the Malacostraca accounting for over 50\%.
The feeding habits of male and female M. caml were
similar. The age of M. caml ranged from 9 to 19 years.
Additionally, with the increase of body size, the
proportion of Cnidaria was decreasing whereas the
proportion of Malacostraca was increasing. The results
would provide a reference for exploring the trophic
level of M. caml and the food web in the Cosmonauts
Sea.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Campbell:2023:GDP,
author = "Matthew R. Campbell and Eric D. Tretter and James C.
Trainer and Richard A. Wilkison",
title = "Genetic Diversity and Population Structure of
{Shoshone} Sculpin \bioname{Cottus greenei} in the
\geoname{Hagerman Valley of South-Central Idaho}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "55",
day = "16",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010055",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/55",
abstract = "The Shoshone sculpin Cottus greenei is a micro-endemic
species and an extreme habitat specialist,
geographically restricted to the spring outlets that
flow from the Snake River Plain Aquifer into the Snake
River within the Hagerman Valley of south central
Idaho. Although previous studies documented the range
of the species and its relative abundance, no studies
have assessed genetic diversity and structure. We
sampled 20 populations from throughout the species
range and genotyped 1311 with a panel of 12
microsatellite loci. Results indicate very high levels
of genetic differentiation among most populations
(average pairwise F ST = 0.24), indicating limited gene
flow. Preservation of the genetic diversity of this
species will require the protection and preservation of
multiple isolated populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Henry-Silva:2023:PPW,
author = "Gustavo Gonzaga Henry-Silva and Joseanna Alves and
Dallas Flickinger and Renata Gomes-Rebou{\c{c}}as and
Ambrosio Bessa-Junior",
title = "Polyculture of \geoname{Pacific} White Shrimp
\bioname{Litopenaeus vannamei} ({Boone}) and Red
Seaweed \bioname{Gracilaria birdiae} ({Greville}) under
Different Densities",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "54",
day = "15",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010054",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/54",
abstract = "The present study evaluated a polyculture system with
Pacific white shrimp (\bioname{Litopenaeus vannamei})
and the macroalga \bioname{Gracilaria birdiae} and its
potential to remove nutrients. The experimental design
consisted of a shrimp monoculture of 100 animals/m 2
(T0) and three multitrophic cultures with L. vannamei
(100 animals/m 2) and with G. birdiae at densities of
500 (T500), 1000 (T1000), and 2000 (T2000) g/m 2.
Nitrogen and phosphorus concentrations decreased at the
beginning of the experiment in the treatments with
macroalgae, but this reduction was not maintained
throughout the cultivation period. The stable values of
G. birdiae biomass were perhaps related to the high
turbidity of the water. There was an increase in shrimp
biomass during cultivation, which reached the final
individual averages of 7.5 g (T0), 7.6 g (T500), 5.9 g
(T1000), and 7.5 g (T2000), with no significant
differences between treatments. Nevertheless, the
absence of macroalgae growth provides no added profit
for the producer. Thus, there is no economic advantage
in using G. birdiae in an integrated multitrophic
system with L. vannamei at a high density and
conditions of high water turbidity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fernandez-Muela:2023:EFI,
author = "Montserrat Fern{\'a}ndez-Muela and Rub{\'e}n
Bermejo-Poza and Almudena Cabezas and Concepci{\'o}n
P{\'e}rez and Elisabet Gonz{\'a}lez de Chavarri and
Mar{\'\i}a Teresa D{\'\i}az and Fernando Torrent and
Morris Villarroel and Jes{\'u}s De la Fuente",
title = "Effects of Fasting on Intermediary Metabolism Enzymes
in the Liver and Muscle of Rainbow Trout",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "53",
day = "14",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010053",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/53",
abstract = "Fish can go through periods of feed deprivation,
either due to natural causes influenced by temperature
or management techniques in animal production,
affecting their energy metabolism differently,
depending on the duration of fasting in days and water
temperature. This study showed the effect of different
days of fasting before slaughter in rainbow trout by
analyzing the intermediary metabolic enzymes in the
muscle and liver. For this purpose, a non-fasting group
was compared with trout that fasted for 5, 10, and 20
days (55, 107, and 200 degree days ({$^\circ $}C d),
respectively). A first phase of increased activity of
enzymes involved in glycolysis was observed, increasing
hexokinase enzyme activity in muscle and pyruvate
kinase enzyme activity in muscle and liver. As the
fasting days progressed, enzymes involved in the
gluconeogenic and glycogenolytic pathways in the liver
such as lactate dehydrogenase and glycogen
phosphorylase increased their activity regarding the no
fasted group. After 20 days of fasting (200 {$^\circ
$}C d), lipogenic and protein related enzyme activity
depletion was observed, leading to significant changes
in energy metabolism. Therefore, prolonged fasting with
high degree days duration may compromise the energy
supply for the vital development of rainbow trout.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gao:2023:TAI,
author = "Jie Gao and Hua-Yang Guo and Ming-Jian Liu and
Ke-Cheng Zhu and Bo Liu and Bao-Suo Liu and Nan Zhang
and Shi-Gui Jiang and Dian-Chang Zhang",
title = "Transcriptome Analysis of the Immune Process of Golden
Pompano (\bioname{Trachinotus ovatus}) Infected with
\bioname{Streptococcus agalactiae}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "52",
day = "13",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010052",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/52",
abstract = "The golden pompano (\bioname{Trachinotus ovatus}) is
one of the most economically valuable marine fishes in
South China. \bioname{Streptococcus agalactiae}, an
infectious Gram-positive bacterium that is highly
destructive for golden pompano culture, has recently
caused massive losses to the golden pompano industry.
This study aimed to investigate the dynamic immune
response of golden pompano to \bioname{S. agalactiae}
infection, using RNA-seq analysis at two different time
points after infection. Abundances of differentially
expressed genes (DEGs) gradually increased in the liver
and spleen 48--120 h post-infection, whereas those in
the head kidney were lower at 120 h than at 48 h.
Pathway enrichment analysis of DEGs revealed that genes
related to the complement system were continuously
transcribed between 48 and 120 h. Metabolic and
immune-regulation-related pathways were highly enriched
in the liver 48 h after infection. Transcriptome
analysis was verified using quantitative PCR for eight
genes with similar expression trends. This study
revealed the inflammatory response of golden pompano
after \bioname{S. agalactiae} infection, including
inflammation-related chemokines and signaling pathways.
Our findings provide a theoretical basis for studying
\bioname{S. agalactiae} resistance in golden pompano
and provide a reliable resource for the genetic
breeding of fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wahltinez:2023:CSS,
author = "Sarah J. Wahltinez and Kevin J. Kroll and Donald C.
Behringer and Jill E. Arnold and Brent Whitaker and
Alisa L. Newton and Kristina Edmiston and Ian Hewson
and Nicole I. Stacy",
title = "Common Sea Star (\bioname{Asterias rubens}) Coelomic
Fluid Changes in Response to Short-Term Exposure to
Environmental Stressors",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "51",
day = "12",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010051",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/51",
abstract = "Common sea stars (\bioname{Asterias rubens}) are at
risk of physiological stress and decline with projected
shifts in oceanic conditions. This study assessed
changes in coelomic fluid (CF) blood gases,
electrolytes, osmolality, and coelomocyte counts in
adult common sea stars after exposure to stressors
mimicking effects from climate change for 14 days,
including decreased pH ({$-$0}.4 units, mean: 7.37),
hypoxia (target dissolved oxygen $ \approx $1.75 mg O 2
/L, mean: 1.80 mg O 2 /L), or increased temperature
(+10 {$^\circ $}C, mean: 17.2 {$^\circ $}C) and
compared sea star CF electrolytes and osmolality to
tank water. Changes in CF blood gases, electrolytes,
and/or coelomocyte counts occurred in all treatment
groups after stressor exposures, indicating adverse
systemic effects with evidence of increased energy
expenditure, respiratory or metabolic derangements, and
immunosuppression or inflammation. At baseline, CF
potassium and osmolality of all groups combined were
significantly higher than tank water, and, after
exposures, CF potassium was significantly higher in the
hypoxia group as compared to tank water. These findings
indicate physiological challenges for A. rubens after
stressor exposures and, given increased observations of
sea star wasting events globally, this provides
evidence that sea stars as a broad group are
particularly vulnerable to changing oceans.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Alvarez:2023:IAI,
author = "Paula Alvarez and Dorleta Garcia and Unai Cotano",
title = "Investigating the Applicability of Ichthyoplanktonic
Indices in Better Understanding the Dynamics of the
Northern Stock of the Population of \geoname{Atlantic}
Hake \bioname{Merluccius merluccius} ({L.})",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "50",
day = "12",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010050",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/50",
abstract = "Abundance indices are essential data for the
application of stock assessment models to obtain fish
abundance estimates. Abundance indices have usually
been derived from fishery-dependent data, yet the
increase in fisheries-independent surveys is now
offering new opportunities for these calculations. In
this study, we explored the usefulness of
ichthyoplankton indices derived from scientific surveys
in estimating spawning biomass. In addition, we also
investigated whether the strength of the year--class of
the commercial cohort of Atlantic hake, as a
determinant, could be defined at an early life stage.
We used samples collected during the triennial mackerel
and horse mackerel egg surveys (MEGS), which cover the
hake spawning area in the Bay of Biscay. The biomass
indices were determined as the abundance of eggs in the
early development stage (stage 1) when transformed into
egg production (EP) from 1995 to 2019 in the months of
March and April---which is considered a period of high
spawning activity for hake in this area. Additionally,
we built a metric for larval abundance and converted
larval length into age. This was in addition to
constructing a pre-recruit year-class index (YCI) while
using the EVHOE bottom trawl abundance database for
hake for the period of 1997 to 2016. The results of
regression analysis of egg production and spawning
stock biomass indicate that both parameters are
significantly correlated (r = 0.76). By connecting the
abundance of eggs and larvae in the adjoining stages,
we are able to identify two periods of high mortality
associated with the transition from ``yolk-sac-first''
to ``feeding larvae'' and ``late larvae-YCI10'', but we
were unable to discover when the strength of the
recruitment year--class is determined. As such, it
appears that for the northern stock of hake,
recruitment is established in the late juvenile
stages.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ndashe:2023:ARF,
author = "Kunda Ndashe and Bernard Mudenda Hang'ombe and Katendi
Changula and John Yabe and Mulemba Tillika Samutela and
Mwansa M. Songe and Alexander Shula Kefi and Loziwe
Njobvu Chilufya and Martin Sukkel",
title = "An Assessment of the Risk Factors Associated with
Disease Outbreaks across Tilapia Farms in
\geoname{Central and Southern Zambia}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "49",
day = "12",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010049",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/49",
abstract = "The study investigated the management practices that
contribute to disease outbreaks in farmed tilapia in
Lusaka and central and southern provinces in Zambia. It
was a cross-sectional qualitative study undertaken from
January to March 2021 in which questionnaires were
administered to 49 farmers to assess their fish health
management and biosecurity competence. Data were
analysed using means, percentages, ratios, and
logistical regression. The results showed that the
majority of the farms had high stocking densities
({$>$8} fish/m 2, 44.4\%), reared Nile tilapia
(67.7\%), and sourced water for farming from rivers and
streams (45.7\%). A few farmers measured water quality
parameters daily (16.7\%) and removed dead fish from
ponds daily (20.8\%). The stocking density ($ p =
0.013$), fish species ($ p = 0.031$), dead fish
disposal methods ($ p = 0.023$), and control of
predator birds ($ p = 0.016$) influenced the total
mortality recorded on farms, while pond type ($ p =
0.031$ and $ p = 0.045$), water source ($ p = 0.023$),
and stocking density ($ p = 0.027$) influenced the
duration of a mortality episode. It is evident that
some fish health management practices and biosecurity
concepts among tilapia farmers in the study area are
inadequate and may not contain disease outbreaks or the
spread of pathogens.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kovacic:2023:CQB,
author = "Ines Kova{\v{c}}i{\'c} and Ante {\v{Z}}unec and Mauro
Mate{\v{s}}kovi{\'c} and Petra Buri{\'c} and Neven
Ive{\v{s}}a and Mauro {\v{S}}tifani{\'c} and Jadranka
Frece",
title = "Commercial Quality, Biological Indices and Biochemical
Composition of Queen Scallop \bioname{Aequipecten
opercularis} in Culture",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "48",
day = "11",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010048",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/48",
abstract = "Market demand for scallops has considerably increased
in recent decades. Although natural populations of
scallops are vulnerable, the queen scallop, Aequipecten
opercularis, can represent a possible alternative to
at-risk species. The aim of this study was to determine
the effect of seawater parameters on the commercial
quality, biological indices and nutritional quality of
the scallop A. opercularis in 1900 L tanks in ex situ
conditions. The condition index (CI) and meat yield
(MY) peaked in autumn (89.92\% and 40.29\%,
respectively). The muscle index (MI) showed the highest
peak during the winter season (5.96\%), while the
gonadosomatic index (GSI) (34.06\%) peaked in the
spring months. Protein content (6.89--9.56 g/100 g),
lipids (2.58--2.79 g/100 g) and carbohydrates
(0.12--0.33 g/100 g) varied during the study period.
Seasonal fluctuations in seawater temperature and pH
negatively influenced the CI, MY and protein and lipid
content, while positively affecting carbohydrate and
moisture content. The most suitable period of the year
for the consumption of the scallop A. opercularis was
found to be the winter and spring periods, when the
scallops achieved their highest nutritional value. It
can be stated from this study that queen scallops in
the Adriatic Sea have a high nutritional quality and
that they can be proposed for higher human
consumption.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Long:2023:EFD,
author = "Qiaolin Long and Jian Liu and Yunfei Sun and Zhigang
Yang and Boping Tang and Yongxu Cheng",
title = "The Effect of Food Deprivation on Foraging Behavior
and Digestive and Metabolic Capacities of the
\geoname{Chinese} Mitten Crab, \bioname{Eriocheir
sinensis}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "47",
day = "11",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010047",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/47",
abstract = "Food deprivation is a common stress in crustaceans
that can affect their behavior and physiology. In this
study, a video recording analysis system was used to
compare the predation rate and behavior of the Chinese
mitten crab, Eriocheir sinensis, foraging on the
freshwater snail, Bellamya quadrata, under different
degrees of food deprivation. The activities of lactate
dehydrogenase, {\alpha}-amylase, pepsin, and lipase in
the hepatopancreas of crabs were determined after food
deprivation for 0, 3, 9, and 15 days. The results
showed that the predation and encounter rates of E.
sinensis increased and then decreased with an increase
in food deprivation time. The proportion of stationary
time of E. sinensis initially decreased and then
increased, whereas the proportion of searching and
handling time increased initially and then decreased.
There was a significant difference in the probability
of capture upon encountering; however, food deprivation
did not significantly affect the probability of
consumption upon capture. Under food deprivation, the
proportion of E. sinensis crushing tactics after
nine-day food deprivation was significantly lower than
that in the first nine days. Lactate dehydrogenase
activity in the E. sinensis hepatopancreas after food
deprivation was significantly higher than that in those
not subjected to food deprivation, and the activities
of {\alpha}-amylase and pepsin in the hepatopancreas
were consistent with the predation rate trend, showing
an initial increase followed by a decrease. In
conclusion, different degrees of food deprivation
significantly affected the predation cycle of E.
sinensis on B. quadrata. These results lay a solid
foundation for further studies on the foraging
behavioral ecology of E. sinensis and provide important
information for improving E. sinensis production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:ECA,
author = "Jiaqi Wang and Chunxia Gao and Feng Wu and Libin Dai
and Qiuyun Ma and Siquan Tian",
title = "Environmental Characteristics Associated with the
Presence of the Pelagic Stingray
(\bioname{Pteroplatytrygon violacea}) in the
\geoname{Pacific} High Sea",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "46",
day = "10",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010046",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/46",
abstract = "The pelagic stingray (\bioname{Pteroplatytrygon
violacea}), perhaps the only stingray to inhabit open
ocean waters, is highly interactive with longline and
purse seine fisheries. The threat to \bioname{P.
violacea} posed by high bycatch mortality has received
widespread attention. To date, the environmental
preference of \bioname{P. violacea}, which is important
in designing conservation and management measures, has
not been well studied. Based on data collected during a
2016--2019 survey in the Pacific Ocean by national
observers of tuna longline fisheries, the relationship
between the presence of \bioname{P. violacea} and
spatiotemporal and environmental variables was first
analyzed using the Generalized Additive Model. The
results showed that geographic location (latitude and
longitude) was the most influential variable. Monthly,
\bioname{P. violacea} is frequently present in the
Pacific high sea from December to May. The El
Ni{\~n}o--Southern Oscillation had a significant impact
on the presence of \bioname{P. violacea} in the Pacific
high sea, with both the cold (Ocean Nino Index $ < -
0.5$) and warm (Ocean Nino Index $ > 1 $) phases
leading to a decrease in its presence. Regarding the
environmental factors, we found that high presence was
associated with low salinity (33.0 $ \approx $ 34.5
psu), a relatively high concentration of chlorophyll
(0.2 -- 0.35 m g / m 3), and warm water ($ > 20^\circ
$C). \bioname {P. violacea} was most likely observed in
the waters offshore, closer to seamounts, and with
water depths between 4000 and 5000 m. Four areas,
including those east of the Solomon Islands and east of
Kiribati, areas west of the Galapagos Islands, and
areas near the coastal upwelling of northern Peru,
related to upwelling systems or seamounts, were
identified as the potential key habitats of \bioname
{P. violacea}. Predicted distribution maps showed a
significant seasonal variation in the presence of
\bioname {P. violacea}. Moreover, the yearly change in
the presence of \bioname {P. violacea} in the Pacific
high sea indicated a possible decreasing trend in
recent years. The information first provided here is
essential for developing conservation and management
measures for \bioname {P. violacea} to prevent the
unavoidable ecological consequences of bycatch or other
anthropogenic factors.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zheng:2023:CAF,
author = "Yueping Zheng and Jiehao Liu and Jianan Xu and Houyong
Fan and Youji Wang and Ping Zhuang and Menghong Hu",
title = "Comparison of Artificial Feed and Natural Food by the
Growth and Blood Biochemistry in \geoname{Chinese}
Sturgeon \bioname{Acipenser sinensis}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "45",
day = "10",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010045",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/45",
abstract = "As an endangered animal species, the Chinese sturgeon
(\bioname{Acipenser sinensis}) has been artificially
cultured during recent years. In the present
experiment, the performance of a common formulated feed
and a mixed natural feed on the growth and blood
physiology of Chinese sturgeon were compared during one
year of indoor culture. In terms of specific growth
rate, the formulated diet group showed a higher value
(4.2\%) compared with the natural food group
({$-$1}.8\%) during one year of indoor culture. Alanine
aminotransferase activities of the sturgeons in
formulated diet group was significantly higher than
that in the natural food group throughout whole
experiment period ($ p < 0.05$). The total protein
albumin, albumin and globulin contents of the natural
food group were significantly higher than that of the
formulated diet group in February and May ($ p <
0.05$). The C-reactive protein contents of the
formulated diet group were significantly higher than
that of natural food group in May and August ($ p <
0.05$). The activities of total cholesterol,
high-density cholesterol, and low-density cholesterol
were significantly higher in the natural food group
than those in the formulated diet group ($ p < 0.05$).
The activities of triglycerides were significantly
higher in the natural food group than those in the
formulated diet group in February and May ($ p <
0.05$). Almost half of the biochemical indicators
examined show significant seasonal fluctuations,
indicating that seasons have a substantial impact on
the Chinese sturgeon. Overall, these results indicate
that natural food exerted a positive impact on
physiological indicators compared to the formulated
diet.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Quigg:2023:FWC,
author = "Antonietta Quigg and R. J. David Wells and Jay R.
Rooker and Ronald L. Hill and Larissa L. Kitchens and
Michael A. Dance and David L. Moulton and Phillip J.
Sanchez and Beatrice Padovani Ferreira",
title = "Food Web Connectivity in a Mangrove--Seagrass--Patch
Reef ({MSP}) Seascape: Lessons from a Tropical
Back-Reef in \geoname{Puerto Rico}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "44",
day = "08",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010044",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/44",
abstract = "Alterations to the functions and connectivity of
mangrove--seagrass--patch reef (MSP) seascapes have the
potential to impact the survival, foraging activities,
and movement of reef-dependent invertebrates (e.g.,
crabs and shrimp) and fishes. In the current study, we
examined carbon flow in the Gu{\'a}nica Biosphere
Reserve in southwestern Puerto Rico using pigment
analysis of particulate organic matter and stable
isotope analysis of carbon ({\delta} 13 C) and nitrogen
({\delta} 15 N) in flora and fauna. Several lines of
evidence pointed to N 2 fixers (cyanobacteria) being
important for fueling primary productivity in this
oligotrophic ecosystem including low ({$<$0}.7 $ \mu $
g L {$-$1}) chlorophyll, prevalence of cyanobacteria
based on pigment signatures, and the isotope signatures
of seagrass and red mangrove leaf tissue (enriched
{\delta} 15 N values) and consumers (depleted {\delta}
15 N values). Food web mixing models based on stable
isotopes ({\delta} 13 C and {\delta} 15 N) revealed
that multiple producers (phytoplankton, benthic
microalgae, seagrasses, etc.) contributed organic
matter to the consumers (zooplankton, invertebrates,
and fishes) in the MSP seascape at the center of the
reserve. Contribution estimates for common benthic
invertebrates (crabs and shrimp) were taxon-specific,
and the highest input was generally linked to
particulate organic matter (POM) and benthic microalgae
(BMA)/seagrass producer categories, although meaningful
mangrove contribution was observed for some taxa.
Similarly, contribution estimates for fishes were
highest for POM and BMA/seagrass, with the latter
producer category being more important for species
known to migrate from mangroves or patch reefs to
seagrass beds at night (bluestriped grunt, French
grunt, and white grunt). Although all fish investigated
were observed in mangrove prop-root habitats, input of
organic matter from mangroves to these consumers was
typically limited for most of the species examined.
Understanding these complex seascapes contributes to
our understanding of the ecology of these vital
ecosystems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zaccone:2023:LAA,
author = "Giacomo Zaccone and Alessio Alesci and Doaa M. Mokhtar
and Marialuisa Aragona and Maria Cristina Guerrera and
Gioele Capillo and Marco Albano and Jorge de Oliveira
Fernandes and Viswanath Kiron and Ramy K. A. Sayed and
Marwa M. Hussein and Patrizia Lo Cascio and Michal
Kuciel and Krystyna Zuwala and Antonino German{\`a} and
Jose Manuel Icardo and Eugenia Rita Lauriano",
title = "Localization of Acetylcholine, Alpha 7-{NAChR} and the
Antimicrobial Peptide Piscidin 1 in the Macrophages of
Fish Gut: Evidence for a Cholinergic System, Diverse
Macrophage Populations and Polarization of Immune
Responses",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "43",
day = "08",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010043",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/43",
abstract = "The recognition and elimination of invading pathogens
are vital for host survival. Macrophages play a central
role in host protection and cells functionally
reminiscent of vertebrate macrophages are present in
all multicellular organisms. A pattern responsible for
bacterial recognition found on the surface of
macrophages is CD14. These cells possess a repertoire
of antimicrobial molecules stored in their granules and
lysosomes. Polarization states observed in mammalian
macrophages termed M1 and M2 also likely exist in fish
macrophages. Markers for macrophage subtypes are slowly
but definitively emerging in fish species. In the
present study cell markers such as CD14, acetylcholine,
alpha 7 acetylcholine nicotinic receptor (nAChR)
subtype, the inducible nitric oxidase synthase (iNOS),
and the antimicrobial peptide piscidin 1 are reported
for the first time in the intestinal macrophages of
both catfish Heteropneustes fossilis (Bloch, 1794) and
the African bonytongue Heterotis niloticus (Cuvier,
1829) along the anterior and the posterior axis and the
concentric muscle layers. Many antimicrobial effector
responses of vertebrate macrophages including
respiratory burst and NO induction are similar across
the diverse animal taxa. Antibodies against calbindin
coupled with ones to VAChT and tubulin revealed the
localization of myenteric and submucosal plexuses,
which are made up of enteric neurons, glial cells, and
nerves near macrophages. Current studies allow for the
elucidation of multiple roles of macrophages in disease
models providing an insight into their in vivo function
in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barnes:2023:CAM,
author = "Megan Barnes and Brad Ebanks and Andrew MacColl and
Lisa Chakrabarti",
title = "A Common Anaesthetic, {MS-222}, Alters Measurements
Made Using High-Resolution Respirometry in the
Three-Spined Stickleback (\bioname{Gasterosteus
aculeatus})",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "42",
day = "07",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010042",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/42",
abstract = "Submersion in the anaesthetic MS-222 is a
well-established and effective method used during the
euthanasia of fish, but the consequences of treatment
with this anaesthetic for mitochondrial respiration are
yet to be established. This is important to evaluate,
as an increasing amount of research is conducting
high-resolution respirometry to measure respiration
across multiple species of fish, including looking at
thermal sensitivity and mitochondrial responses to the
warmer temperatures faced with climate change. Analysis
often occurs after euthanasia with MS-222 without
knowledge of how MS-222 itself affects any measured
parameters of mitochondrial respiration, leaving
potential for a misinterpretation of results. Here,
high-resolution respirometry was conducted to explore
how MS-222 affects oxidative phosphorylation in the
brain and skeletal muscle of the three-spined
stickleback, Gasterosteus aculeatus, which is a model
species in evolutionary ecology. In the brain,
differences in respiration were observed between
three-spined sticklebacks euthanised with MS-222 and
those where no anaesthetic was implemented. No
differences between treatments were observed in the
skeletal muscle, although variation between individuals
was high and oxygen flux was lower than in the brain.
Overall, this study highlights the need for a
consistent method of euthanasia when conducting
high-resolution respirometry in fish, as MS-222 may
alter measures of oxidative phosphorylation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Przybyla-Kelly:2023:RGD,
author = "Kasia J. Przybyla-Kelly and Ashley M. Spoljaric and
Meredith B. Nevers",
title = "Round Goby Detection in \geoname{Lakes Huron and
Michigan} --- an Evaluation of {eDNA} and Fish
Catches",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "41",
day = "06",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010041",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Przybyla-Kelly:2023:CPK}.",
URL = "https://www.mdpi.com/2410-3888/8/1/41",
abstract = "Aquatic surveys for fish in large water bodies (e.g.,
Laurentian Great Lakes of North America) often require
a flexible approach using multiple methods, surveying
different depths, and sampling across seasons,
especially when the target species is elusive in its
natural habitat. The round goby (\bioname{Neogobius
melanostomus}) is an invasive, bottom-dwelling fish
inhabiting rocky areas of all five Great Lakes. While
trawl surveys are typically used for abundance
assessments, angling has been demonstrated as a means
of supplementing surveys with additional data. Yet,
round goby abundance and distribution is still not well
described. Recently, with considerable success,
scientists have explored sampling environmental DNA
(eDNA) to complement traditional monitoring techniques
for population abundance estimates, early detection of
invasive species, and spawning or migration events.
Therefore, we collected eDNA from water samples
alongside bottom trawls and hook and line angling in
Lakes Huron and Michigan to detect round goby. eDNA
samples were analyzed by both droplet digital PCR
(ddPCR) and quantitative PCR (qPCR) to maximize the
likelihood of detection. Overall, round goby was
captured in 23\% of the trawls, but the eDNA based
methods detected round goby in 74\% and 66\% of samples
by ddPCR and qPCR, respectively, mostly in samples
collected at {$<$30} m depths, and mostly in the fall.
More studies comparing eDNA based methods to
traditional monitoring, especially trawls in large open
waters, may contribute to a better understanding of
using eDNA in population assessments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kang:2023:SSE,
author = "Chang-Keun Kang and Hyun-Woo Kim and Hyun Je Park and
Chung Il Lee",
title = "Sharing Scientific Evidence of the Response of Aquatic
Animals to Environmental Change",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "40",
day = "05",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010040",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/40",
abstract = "Aquatic animals, which are ecologically important
consumers in aquatic ecosystems and widely distributed
in rivers, lakes, and seas, which occupy 70\% of the
earth's surface, respond in unique ways to
environmental changes at various temporal and spatial
scales, and have optimized strategies for survival
\ldots{}",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Samy-Kamal:2023:DMS,
author = "Mohamed Samy-Kamal and C{\'e}lia M. Teixeira",
title = "Diagnosis and Management of Small-Scale and
Data-Limited Fisheries",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "39",
day = "05",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010039",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/39",
abstract = "Aquatic animals, which are ecologically important
consumers in aquatic ecosystems and widely distributed
in rivers, lakes, and seas, which occupy 70\% of the
earth's surface, respond in unique ways to
environmental changes at various temporal and spatial
scales, and have optimized strategies for survival
\ldots{}",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vercelli:2023:TRI,
author = "Cristina Vercelli and Michela Amadori and Massimiliano
Tursi and Graziana Gambino and Paolo Pastorino and
Marino Prearo and Ugo Ala and Raffaella Barbero and
Giovanni Re",
title = "{TRPV1} Receptor Identification in Rainbow Trout
(\bioname{Oncorhynchus mykiss}) and Evaluation of the
Effects Produced by \bioname{Ocimum basilicum} Super
Critical Fluid Extract",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "38",
day = "04",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010038",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/38",
abstract = "Transient receptor potential vanilloid type 1 (TRPV1)
has been investigated in humans and mammals; in recent
years, some researchers have focused on this receptor
in fishes. The present study aimed to identify TRPV1
receptors in cultures of RT-gill W1 cells and in the
organs of rainbow trout (\bioname{Oncorhynchus
mykiss}), in addition to evaluating the possible
modulation induced by super critical fluid extract of
basil (\bioname{Ocimum basilicum}), named F1-BEO. In
vitro evaluation consisted of cell cultures and
immunocytochemistry assays. During in vivo experimental
sessions, eighty trout were divided into five groups
that received a fish diet supplemented with 0, 0.5, 1,
2 and 3\% w / w F1-BEO. Forty trout were euthanized
after 15 and 30 days; organs were collected and
processed according to the immunohistochemistry
technique. Receptor expression was quantitatively
measured using Image Pro Plus software. TRPV1 was
identified in RT-gill W1 cells and in all organs, with
a higher positivity in the muscle layers of the
stomach, intestine and kidneys. F1-BEO induced an
increased expression of TRPV1 in the stomach while a
lower expression was appreciated in the bowel. No
morphological alterations have been highlighted in the
liver or kidneys. Further investigation will be
necessary to evaluate the functionality of this
receptor in rainbow trout.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gauthier:2023:TCR,
author = "Nath{\'a}lia Byrro Gauthier and Filipe Scavone
G{\'o}es and Virg{\'\i}nia Fonseca Pedrosa and
F{\'a}bio Roselet and Luis Alberto Romano and Ronaldo
Olivera Cavalli",
title = "Towards the Control of the Reproduction of the Yellow
Clam \bioname{Amarilladesma mactroides} ({Reeve}, 1854)
in Captivity: Effects of Different Stimuli on the
Spawning of Laboratory-Conditioned and Unconditioned
Breeders",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "37",
day = "04",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010037",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/37",
abstract = "The effects of temperature manipulation, addition of
sperm solution, and exposure to alkalized pH and/or
hydrogen peroxide (H 2 O 2) as possible spawning
inducers in laboratory-conditioned and unconditioned
adults of the yellow clam (\bioname{Amarilladesma
mactroides}) were evaluated. In three trials, clams
were laboratory-conditioned for 14 days and exposed to
thermal shocks (from 20 {$^\circ $}C to 23--29 {$^\circ
$}C), while clams from three additional trials were not
conditioned but acclimatized for 45 min before spawning
induction. Although conditioning advanced gonad
maturity and increased the condition factor, none of
the thermal treatments triggered spawning in these
first trials. Histological analysis indicated that the
gonads of conditioned clams were not mature.
Alternatively, unfertilized and fertilized eggs, and
larvae were observed after unconditioned clams were
induced to spawn. The gonads of unconditioned clams
were in an advanced stage of maturity. Exposure to
temperature shocks, alone or in combination with the
addition of sperm solution and with H 2 O 2, resulted
in spawning. Clams exposed to H 2 O 2 kept their valves
closed and had a high mortality rate. Temperature
manipulation is the most promising stimulus to induce
spawning in A. mactroides. As the success of
conditioning depends on the stage of gonadal
development at the time of capture in the wild, a
period longer than 14 days may be necessary.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:IUU,
author = "Xidi Chen and Qi Xu and Lun Li",
title = "Illegal, Unreported, and Unregulated Fishing
Governance in Disputed Maritime Areas: Reflections on
the International Legal Obligations of States",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "36",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010036",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/36",
abstract = "Illegal, unreported, and unregulated (IUU) fishing in
the disputed maritime areas causes significant damage
to the marine ecology and authorized fisheries,
increases the risk of conflicts among disputed states,
and violates human rights at sea. Both unilateral
measures and cooperative governance for IUU fishing are
often inadequate in these areas. In light, this study
aims to clarify the regulatory obligations of relevant
states and explore feasible solutions based on
international cooperation to promote IUU governance in
disputed areas worldwide. The rapidly evolving
international fisheries legal framework requires that
states, such as coastal states, flag states, port
states, or market states, fulfill their respective
obligations to prevent and deter IUU and that the
presence of disputes in a specific maritime area does
not typically constitute grounds for derogation from
these obligations or exemption from possible state
responsibility. However, the implications of the
conflicting claims in disputed maritime areas should be
taken into consideration while interpreting and
applying international legal rules. Therefore, this
study suggests that regional and inter-regional
cooperation is necessary for states to fulfill their
obligations to regulate IUU fishing and prevent state
responsibilities under international law. Parties to
the dispute, as well as third parties, are encouraged
to participate in the cooperative mechanism in order to
coordinate legislative and enforcement measures and
advance the institutionalization of IUU fishing
regulation in the disputed maritime areas, which will
not only advances the effective governance of IUU
fishing but also reduces tensions among the disputing
states and contributes to the peaceful settlement of
the dispute.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ferri:2023:OTA,
author = "Josipa Ferri",
title = "Otoliths and Their Applications in Fishery Science",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "35",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010035",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/35",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zuo:2023:EUF,
author = "Chenxia Zuo and Tingting Zhang and Chenchen Zhang and
Daotan Zhao and Yi Zhu and Xiaojie Ma and Haiyan Wang
and Peizhen Ma and Zhen Zhang",
title = "Evaluating the Utility of Five Gene Fragments for
Genetic Diversity Analyses of \bioname{Mytella
strigata} Populations",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "34",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010034",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/34",
abstract = "Mytella strigata (Hanley, 1843) is an invasive mussel
species that has rapidly spread in China in recent
years. Here, we tested the utility of three
mitochondrial gene fragments, COI, 12S, and 16S, and
two nuclear gene fragments, D1 28S and 18S-ITS1, for
characterizing the levels of genetic diversity among
and within populations using 191 M. strigata specimens
collected in China to aid ongoing efforts to identify
the origin of the invasion as well as molecular genetic
studies. M. strigata exhibited two sex-associated
haplogroups according to the COI and 12S sequences. The
ratio of female-lineage to male-lineage COI and 12S
sequences was 149:22 and 72:7, and the genetic
distances between haplogroups were 6.56 and 9.17,
respectively. Only one haplotype was detected among the
18S-ITS1 sequences (413 bp), and three haplotypes were
detected among the D1 28S sequences (296 bp). The
haplotype diversity of both the female-lineage COI and
12S sequences was greater than 0.5, and the nucleotide
diversity of the 12S, 16S, D1 28S, and 18S-ITS1
sequences was less than 0.005 in all six populations in
China. Our findings indicated that COI is the most
useful gene fragment for genetic diversity studies of
M. strigata populations; D1 28S and 18S-ITS1 sequences
would be useful for species identification because of
their low intraspecific diversity. Our genetic analysis
of the COI sequences revealed Colombia as the most
likely origin of M. strigata in China and showed that
the invasive populations in China have recently
experienced or are currently experiencing a population
bottleneck.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huang:2023:CMC,
author = "Zengchao Huang and Liwen Xu and Shiping Yang and
Shuanghu Cai and Jichang Jian and Yucong Huang",
title = "A Case of Mycobacteriosis in Cultured
\geoname{Japanese} Seabass (\bioname{Lateolabrax
japonicus}) in \geoname{Southern China}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "33",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010033",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/33",
abstract = "Japanese seabass (\bioname{Lateolabrax japonicus}) is
an important species of cultured marine fish with high
economic value in China. Nevertheless, from May to
November 2019, mass mortality among cultured Japanese
seabass occurred in Zhuhai City, Guangdong Province of
China. Approximately 0.2--0.5\% mortality was recorded
daily, and the cumulative mortality was up to 30\%
during this disease outbreak. In this study, the
clinical signs and pathological characteristics of
diseased fish were investigated. Furthermore, the
pathogenicity and antibiotic sensitivity of identified
pathogenic bacteria from diseased fish were analyzed.
The infected fish showed clinical signs of
uncoordinated swimming; anorexia; pigment changes; and
a number of 1--5 mm grayish-white nodules in the liver,
spleen, and kidney tissues was also found. A bacterial
strain, which was designated as ZHLJ2019, was isolated
from the diseased fish. To ensure that ZHLJ2019 isolate
was the causative agent, a Koch postulate trial was
performed. Healthy Japanese seabass were infected by
the intraperitoneal injection of 5 $ \times $ 10 4, 5 $
\times $ 10 5 and 5 $ \times $ 10 6 CFU/fish, and
cumulative mortalities within 42 days were 75\%, 90\%,
and 100\%, respectively. The bacteria colony had
traditional morphological and biochemical
characteristics similar to that of Mycobacterium
marinum. Phylogenetic molecular analyses of 16S rRNA,
rpoB, hsp65, erp, and ITS genes confirmed that the
isolated strain ZHLJ2019 was M. marinum. The
granulomatous inflammation in internal organs of
Japanese seabass naturally and experimentally infected
with ZHLJ2019 isolate was consistent with the classic
pathological features of mycobacteriosis. Drug
susceptibility of ZHLJ2019 isolate to 11 antibiotics
was determined by broth dilution method in vitro. The
minimum inhibitory concentrations (MICs) of
minocycline, rifampicin, ethambutol, isoniazid,
streptomycin, doxycycline hydrochloride, kanamycin
sulfate, levofloxacin, roxithromycin, and prothionamide
against the strain ZHLJ2019 were 4, 2, 8, 4, 16, 8, 8,
8, 4, and 8 {\mu}g/mL, respectively. The results of
this study suggest that M. marinum is the causal agent
responsible for the morbidity and mortality of Japanese
seabass cultured in intensive brackish water dirt ponds
in southern China.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:IRB,
author = "Junwei Li and Suwen Chen and Peng Wu and Changbo Zhu
and Ruiping Hu and Ting Li and Yongjian Guo",
title = "Insights into the Relationship between Intestinal
Microbiota of the Aquaculture Worm \bioname{Sipunculus
nudus} and Surrounding Sediments",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "32",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010032",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/32",
abstract = "Sipunculus nudus is an important intertidal
aquaculture species that can ingest organic matter from
the surface sediment and shows a high transportation
capacity in sediment. However, little is known about
the influence of intertidal aquaculture species on the
sediment microbial community and the exchange of
microbiota between the intestine and the surrounding
sediment. In this study, the microbial communities in
the intestine of S. nudus and three kinds of
surrounding sediments were analyzed using
high-throughput sequencing of the 16S rRNA gene
amplicon, and the relationships between different
communities were examined. Principal coordinate
analysis and ANOSIM/Adonis analysis showed that the
microbial communities of worm intestine samples were
significantly different from those of surrounding
sediments ($ p < 0.05$). Meanwhile, compared with the
sediment samples, the microbial {\alpha}-diversity was
significantly lower in the intestinal samples. Although
the relative abundances of Proteobacteria and
Cyanobacteria were high in all samples, three phyla
(Bacteroidetes, Gemmatimonadetes, and Latescibacteria)
showed a great difference between the four groups, as
the abundances of the three phyla were significantly
lower in the intestinal samples. Moreover, several
microbial interactions were found between the worm
intestine and surrounding sediments. BugBase functional
prediction analysis indicated that the oxygen status of
the sediment and the intestine was changed by
bioturbation by the worm. Therefore, the
microenvironment and microbial community in sediment
were affected by the activity of S. nudus in the
intertidal aquaculture zone.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sorensen:2023:HRJ,
author = "Karl Sorensen and Steven R. Craig and Avner Cnaani and
Ewen McLean",
title = "Hematological Response of Juvenile Cobia to Three
Anesthetics",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "31",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010031",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/31",
abstract = "Optimal concentrations of three anesthetics for use
with juvenile cobia (Rachycentron canadum) were
determined using time-to-recovery and hematological
datasets. Buffered MS-222, clove oil and
2-phenoxyethanol (2-PE) were examined. Juvenile cobia
were exposed to three concentrations of each anesthetic
at 24 {$^\circ $}C. Based on time to recovery, optimal
doses for MS-222 was determined as 120-mg L {$-$1},
that for 2-PE, 0.4-mL L {$-$1} and, for clove oil, 5-mL
L {$-$1}. The hematological response of cobia to
anesthesia included quantification of whole blood pH, p
CO$_2$, p O 2, and Ca 2+, K +, Na +, Cl {$-$},
hematocrit and glucose. Irrespective of anesthetic
employed, cobia expressed metabolic acidosis, with
changes in blood pH ($ p < 0.001$) being matched by
increases ($ p < 0.001$) in p CO$_2$. Anesthesia tended
to increase blood Na +, hematocrit, p O 2, Ca 2+, and K
+ although differential responses between anesthetics
were recorded, suggesting different modes of action. A
combination of recovery and hematological data
indicated that when anesthesia is necessary, MS-222
represents the sedative of choice.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2023:EIA,
author = "Jicheng Yang and Yaoyao Lin and Zhaohui Wei and
Zhenbing Wu and Qianqian Zhang and Jingwen Hao and
Shuyi Wang and Aihua Li",
title = "\bioname{Edwardsiella ictaluri} Almost Completely
Occupies the Gut Microbiota of Fish Suffering from
Enteric Septicemia of Catfish ({Esc})",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "30",
day = "03",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010030",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/30",
abstract = "To reveal the changes in the gut microbiota of yellow
catfish after being infected by Edwardsiella ictaluri,
16S rRNA gene high-throughput sequencing technology was
used to analyze the microbial composition and diversity
of the healthy and diseased yellow catfish. The gut
microbial richness and diversity of the diseased fish
were significantly lower than those of healthy fish.
The composition and predicted function of yellow
catfish gut microbiota were drastically altered after
infection by E. ictaluri. Fusobacteriota,
Proteobacteria, and Firmicutes were the predominant
bacterial phyla in the gut of the healthy fish, while
Proteobacteria was the dominant phylum in the gut of
the diseased fish. At the genus level, the gut of
healthy fish was dominated by Cetobacterium,
Plesiomonas, and Romboutsia, while the gut of diseased
fish was overwhelmed by the pathogenic E. ictaluri
(99.22 {\pm} 0.85\%), and Cetobacterium, Plesiomonas,
and Romboutsia disappeared. This is the most
characteristic feature of the intestinal microbiota
composition of yellow catfish edwardsiellosis. The same
sequence of E. ictaluri was detected in the intestine
of the healthy fish and the liver and intestine of the
diseased fish. The anaerobic and Gram-positive bacteria
were significantly decreased, and the digestive system,
immune system, and metabolic functions of the gut
microbiota were significantly reduced in the diseased
fish gut. This may be part of the pathogenesis of fish
edwardsiellosis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Belkin:2023:RSS,
author = "Igor M. Belkin and Xin-Tang Shen",
title = "Remote Sensing of the Subtropical Front in the
\geoname{Southeast Pacific} and the Ecology of
\geoname{Chilean} Jack Mackerel \bioname{Trachurus
murphyi}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "29",
day = "02",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010029",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/29",
abstract = "The Subtropical Front (STF) plays a key role in the
ecology of Chilean jack mackerel Trachurus murphyi.
Nonetheless, there are few remote sensing studies of
the STF in the open Southeast Pacific, and almost all
of them have been conducted by satellite oceanographers
in Russia and Ukraine to support respective large-scale
fisheries of jack mackerel in this region. We reviewed
these studies that documented long-term seasonal and
interannual variability of the STF from sea surface
temperature (SST) and sea surface height (SSH) data. We
also mapped the STF from satellite sea surface salinity
(SSS) data of the SMOS mission (2012--2019). The
Subtropical Front consists of two fronts--North and
South STF about 500 km apart--that border the
Subtropical Frontal Zone (STFZ) in-between. The STF is
density-compensated, with spatially divergent
manifestations in temperature and salinity. In the
temperature field, the STF extends in the WNW to ESE
direction in the Southeast Pacific. In the salinity
field, the STFZ appears as a broad frontal zone,
extending zonally across the entire South Pacific.
Three major types of satellite data-SST, SSH, and
SSS-can be used to locate the STF. The SSH data is most
advantageous with regard to the jack mackerel
fisheries, owing to the all-weather capability of
satellite altimetry and the radical improvement of the
spatial resolution of SSH data in the near future.
Despite the dearth of dedicated in situ studies of the
South Pacific STFZ, there is a broad consensus
regarding the STFZ being the principal spawning and
nursing ground of T. murphyi and a migration corridor
between Chile and New Zealand. Major data/knowledge
gaps are identified, and key next steps are proposed to
mitigate the data/knowledge gaps and inform fisheries
management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Eckelbecker:2023:RCP,
author = "Robert W. Eckelbecker and Nathaniel M. Heili and
Christopher S. Guy and David A. Schmetterling",
title = "Relative Condition Parameters for Fishes of
\geoname{Montana, USA}",
journal = j-FISHES,
volume = "8",
number = "1",
pages = "28",
day = "31",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8010028",
ISSN = "2410-3888",
bibdate = "Sat Feb 18 07:10:33 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/1/28",
abstract = "Body condition indices are commonly used in the
management of fish populations and are a surrogate to
physiological attributes such as tissue-energy
reserves. Relative condition factor (K n) describes the
condition of species relative to populations in a
geographic area. We developed models to allow for the
calculation of K n in Montana, USA by using the
weight--length data collected by Montana Fish, Wildlife
\& Parks. We generated log 10 weight--log 10 length
relationships to obtain Montana specific parameter
estimates for relative condition equations (W\prime)
for 51 species and three subspecies. We developed
separate models by water type (e.g., lotic and lentic)
and sex for five species due to varying growth based on
sexual dimorphism and varying ecosystem types. Relative
condition offers the advantage of describing body
condition relative to species in Montana, provides a
condition index for species that do not have
standard-weight models developed for relative weight
(Wr), and affords more information for the global
database on weight--length relationships of fishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2023:SRG,
author = "Zhilong Liu and Tuo Wang and Peng Liu and Dongneng
Jiang and Xi Liu and Siping Deng and Tianli Wu and Yang
Huang and Chunhua Zhu and Guangli Li and Mouyan Jiang",
title = "Selection of Reference Gene for Expression Studies in
the Ovary and Pituitary of Spotted Scat
(\bioname{Scatophagus argus}) at Different Ovarian
Stages",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "120",
day = "20",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020120",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/120",
abstract = "The spotted scat (Scatophagus argus) is the most
widely commercially cultured fish in eastern and
southern Asia that possess potential economic value.
However, up to date, there are no studies reported on
the selection of suitable reference genes in this fish,
which is crucial for guaranteeing accurate and reliable
results in RT-qPCR analyses. This study aimed at
screening the most stable reference genes in the ovary
and pituitary at different ovarian stages, which is of
great significance for further research on the
reproductive regulation mechanism of female S. argus.
In this study, we selected twenty-one genes as the
candidate reference genes, including four genes in the
ovary (EEF1A, ACTB, B2M, and HPRT1), and seventeen
genes in the pituitary (B2M, APOA1, CGBA, RPLP0, GNRHR,
GUSB, TBP, SLC25A5, RPL4, PLA1A, GAPDH, GNB2L1, CTFS,
HPRT1, RPS2, TFRC, and TUBB4B). To analyze the
stability of the potential reference genes, we first
used four commonly used software, BestKeeper, GeNorm,
NormFinder, and {\Delta}Ct, and then we established
comprehensive rankings with ReFinder. The results
indicate that RPL4 and GNB2L1, and EEF1A and HPRT1 were
the most appropriate reference genes for the pituitary
and ovary of S. argus, respectively, in the three
developmental stages of the ovary. Additionally, GeNorm
analysis suggested that for the accurate normalization
of gene expression, two reference genes were reliable
in the ovary and pituitary of S. argus. To the best of
our knowledge and understanding, this is the first
study to identify appropriate reference genes for
analyzing gene expression in S. argus. Consequently,
this study can be used as a foundation for future
research on the reproduction of S. argus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fedorcak:2023:TCG,
author = "Jakub Fedor{\v{c}}{\'a}k and Radek {\v{S}}anda and
Tihomir Stefanov and Jan Mendel and Michal Nowak and
Peter Kri{\v{z}}ek and Anabel Perdices and Jasna
Vuki{\'c} and J{\'a}n Ko{\v{s}}{\v{c}}o",
title = "The {``True} Colours'' of Golden Loaches ({Teleostei:
Cobitidae})",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "119",
day = "20",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020119",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/119",
abstract = "Loaches of the genus Sabanejewia are model organisms
for many ichthyologic studies focusing on morphological
and colour variation. The bright lateral spots---a part
of the mimicry of this fish---cause a taxonomic puzzle.
Common practice is that morphometric and molecular
studies lack habitat assessments of species originating
from different environments. In this study, we analysed
body shape and colour variation within two species of
Sabanejewia in order to reveal whether they are more
affected by phylogeny or environmental conditions. The
environmental parameters were obtained using satellite
mapping tools, body shape using geometric morphometry,
and the molecular identification of specimens using an
analysis of mitochondrial DNA (sequence of the
cytochrome b gene). Generalized linear mixed models
were used for the analysis of lateral spot numbers, and
principal components analysis to describe the changes
in landmark position defined by the permutation tests.
Results show a significant impact of the locality
elevation and specimen's sex on the change of the body
shape. We identified significant differences in the
number of lateral spots, which are considered mimicry
variation across an elevation gradient. Morphological
differences of Sabanejewia spp. vary with ecological
factors along the river continuum.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xing:2023:CCM,
author = "Lili Xing and Lingyu Wang and Femke Roos and Michelle
Lee and Gregory A. Wray",
title = "{CRISPR\slash \gene{Cas9}}-Mediated Disruption of
\gene{Endo16} Cis-Regulatory Elements in Sea Urchin
Embryos",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "118",
day = "20",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020118",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/118",
abstract = "Sea urchins have become significant mariculture
species globally, and also serve as invertebrate model
organisms in developmental biology. Cis-regulatory
elements (enhancers) control development and physiology
by regulating gene expression. Mutations that affect
the function of these sequences may contribute to
phenotypic diversity. Cis-regulatory targets offer new
breeding potential for the future. Here, we use the
CRISPR/Cas9 system to disrupt an enhancer of Endo16 in
developing Lytechinus variegatu s embryos, in
consideration of the thorough research on Endo16' s
regulatory region. We designed six gRNAs against Endo16
Module A (the most proximal region of regulatory
sequences, which activates transcription in the vegetal
plate and archenteron, specifically) and discovered
that Endo16 Module A-disrupted embryos failed to
undergo gastrulation at 20 h post fertilization. This
result partly phenocopies morpholino knockdowns of
Endo16. Moreover, we conducted qPCR and clone
sequencing experiments to verify these results.
Although mutations were not found regularly from
sequencing affected individuals, we discuss some
potential causes. In conclusion, our study provides a
feasible and informative method for studying the
function of cis-regulatory elements in sea urchins, and
contributes to echinoderm precision breeding technology
innovation and aquaculture industry development.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ferragut-Perello:2023:ECS,
author = "Francesca Ferragut-Perello and Sergio
Ram{\'\i}rez-Amaro and Athanassios C. Tsikliras and
Natalia Petit-Marty and Donna Dimarchopoulou and Enric
Massut{\'\i} and Alba Serrat and Francesc Ordines",
title = "Exploitation and Conservation Status of the Thornback
Ray (\bioname{Raja clavata}) in the \geoname{Balearic
Islands (Western Mediterranean)}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "117",
day = "19",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020117",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/117",
abstract = "Fisheries management requires the best scientific
knowledge on the exploitation and conservation status
of stocks. This is challenging for by-catch species,
for which necessary data for their assessment is more
limited than for target ones. That is the case for the
thornback ray, Raja clavata, in the Balearic Islands
(western Mediterranean), whose exploitation and
conservation status are addressed here for the first
time, in an integrative approach, by using innovative
assessment methods and genetic diversity analysis. The
time series of fishing landings from 1964 and MEDITS
trawl survey data from 2004 were used to apply CMSY++
and Bayesian state-space Schaefer production models. We
also estimated the genetic diversity of the Cytochrome
C Oxidase subunit I mitochondrial gene from 31
individuals collected during MEDITS surveys. The
species showed overexploitation with a current fishing
mortality (F) below that producing maximum sustainable
yield (F MSY), but with a current stock biomass (B)
lower than B MSY. Despite the fact that the
exploitation status is stable or even has improved in
the last decades, the overexploitation period since the
late 1970s seems to have reduced levels of genetic
diversity, below those of related species and other
populations of R. clavata. This low genetic diversity
may entail the loss of adaptive potential, making the
species more vulnerable to fishing and the current
climate change.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Khalil:2023:EMR,
author = "Karim Khalil and Ahmed Elaswad and Hisham Abdelrahman
and Maximillian Michel and Wenbiao Chen and Shikai Liu
and Ramjie Odin and Zhi Ye and David Drescher and Khoi
Vo and William S. Bugg and Guyu Qin and Yujia Yang and
Nathan J. C. Backenstose and Zhanjiang Liu and Roger D.
Cone and Rex Dunham",
title = "Editing the Melanocortin-4 Receptor Gene in Channel
Catfish Using the {CRISPR-\gene{Cas9}} System",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "116",
day = "18",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020116",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/116",
abstract = "The melanocortin-4 receptor (MC4R) plays a critical
role in homeostasis and the regulation of body weight.
Polymorphisms in the mc4r gene have been discovered and
linked to growth, carcass composition, and meat quality
traits. Therefore, we used the CRISPR-Cas9 system to
target the mc4r gene in the most important freshwater
aquaculture species in the USA, channel catfish,
Ictalurus punctatus. Guide RNAs were designed to direct
the Cas9 to the coding sequence of the channel catfish
mc4r gene. gRNA(s)-Cas9 mixtures were delivered into
one-cell embryos using electroporation and
microinjection. For each treatment, the nature and rate
of mutations were analyzed. Hatching and survival rates
were calculated. The overall mutation rates were 30.6\%
and 66.7--90.6\% for electroporation and
microinjection, respectively. Mutated fish generated
via electroporation or microinjection exhibited 38\%
and 20\% improvement in body weight, respectively, when
compared with the full-sib control. The mean feed
conversion ratio of the mutants was 1.18 compared with
1.57 in the control fish. The improved growth and feed
conversion indicate that the generation of mc4r -edited
fish could economically benefit aquaculture
production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Aarstad:2023:NAF,
author = "Jarle Aarstad and Stig-Erik Jakobsen and Arnt
Fl{\o}ysand",
title = "\geoname{Norwegian} Aquaculture Firms' Emphasis on
Environmental and Social Sustainability Compared to
Firms in Other Industries",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "115",
day = "17",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020115",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/115",
abstract = "Background: There has been a strong focus on
environmental sustainability in the aquaculture
industry, but we do not know how firms in this industry
emphasize the issue compared to firms in other
industries. Methods: Responding to this research gap,
we compared survey data of Norwegian firms in the (1)
aquaculture industry with those in the (2)
manufacturing industry, (3) the consulting, finance and
insurance industry, and (4) the hospitality, tourism
and culture industry. Results: We found that firms in
the aquaculture industry emphasize environmental
sustainability more than those in the other industries
do. Likewise, firms in the aquaculture industry
emphasize social sustainability more than those in the
other industries do, but the difference is less marked.
Across industries, knowledge-intensive firms emphasize
environmental sustainability and social sustainability
to a lesser extent. Additionally, firms with local or
regional major ownership emphasize social
sustainability more than those with international major
ownership do. Conclusion: Firms in the aquaculture
industry have a relatively strong emphasis on
environmental and social sustainability, which may be
due to the industry's environmental challenges.
Similarly, aquaculture firms have a relatively strong
emphasis on social sustainability, which may be due to
the industry's geographic and sociocultural proximity
to numerous small local communities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2023:CRS,
author = "Kyung-Ho Kim and Min-Soo Joo and Gyoungsik Kang and
Won-Sik Woo and Min-Young Sohn and Ha-Jeong Son and
Chan-Il Park",
title = "Characterization of Red Sea Bream (\bioname{Pagrus
major}) Interferon Regulatory Factor 5 and 6 Genes and
Their Expression in Response to {RSIV} Infection",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "114",
day = "16",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020114",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/114",
abstract = "Interferon regulatory factors (IRFs) play crucial
roles in antiviral processes, such as in the
transcriptional induction of interferon (IFN) and
IFN-stimulated genes (ISGs). In this study, the genes
encoding IRF5 and IRF6 were identified in Pagrus major,
and their expression in various organs after pathogen
infection was analyzed. In the coding sequences of P.
major (Pm) IRF5 and PmIRF6, the DNA binding domain, IRF
association domain, and viral-activated domain were
found to be highly conserved. Phylogenetic analysis
revealed that PmIRF5 and PmIRF6 were most closely
related to IRF5 and IRF6 of large yellow croakers. The
mRNAs for PmIRF5 and PmIRF6 were constitutively
expressed in all organs analyzed but were highly
expressed in the liver and gills. As a result of an
infection with red sea bream iridovirus, a major
pathogen of red sea bream, PmIRF5 and PmIRF6 expression
was significantly upregulated in the spleen and kidney.
On the basis of these results, it can be concluded that
IRF5 and IRF6 expression play an influential role in
the immune system of red sea bream infected with
viruses.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2023:SMR,
author = "Dong In Kim",
title = "A Study on the Metabolic Rate Change Pattern in
{F$_2$} Hybrid Sturgeon, the Bester (\bioname{Huso
huso} $ \times $ \bioname{Acipenser ruthenus}), during
the Early Developmental Stage",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "113",
day = "15",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020113",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Kim:2023:CKD}.",
URL = "https://www.mdpi.com/2410-3888/8/2/113",
abstract = "The primary goal in aquaculture is to maximize the
growth and survival of farmed fishes at a minimal cost,
which requires an understanding of the changes in
metabolism undergone by different fish breeds during
development. In particular, highly intensive
aquaculture production systems, such as recirculating
aquaculture systems, require a better understanding of
oxygen consumption. Ontogenetic phase shifts (i.e.,
sudden changes) in metabolism have been observed in
several aquaculture species during early development,
and such metabolic phase shifts may help to predict the
oxygen consumption of aquaculture species during
different stages of their development. Here, I analyzed
the pattern of metabolic changes in the F 2 hybrid
sturgeon, the bester (Huso huso $ \times $ Acipenser
ruthenus; hereafter, referred to as the F 2 bester),
during its early development. I observed ontogenetic
phase shifts in metabolism in the F 2 bester at body
mass values of about 0.2 and 0.8 g. Thus, the F 2
bester undergoes ontogenetic phase shifts in metabolism
during early development, which can help to
characterize oxygen consumption at a specific
developmental period. Therefore, oxygen can be
appropriately adjusted and replenished during
breeding.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hallerman:2023:GBS,
author = "Eric M. Hallerman",
title = "Genetics and Biotechnology --- The {Section
Editor-in-Chief}'s View",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "112",
day = "15",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020112",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/112",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{MacedoSantana:2023:FVS,
author = "Thiago {Macedo Santana} and Francisco {de Matos
Dantas} and Driely Kathriny {Monteiro Dos Santos} and
Juliana {Tomomi Kojima} and Yugo {Moraes Pastrana} and
Rog{\'e}rio {Souza De Jesus} and Ligia {Uribe
Gon{\c{c}}alves}",
title = "Fish Viscera Silage: Production, Characterization, and
Digestibility of Nutrients and Energy for Tambaqui
Juveniles",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "111",
day = "14",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020111",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/111",
abstract = "Fish viscera can be an important source of protein and
energy for aquafeed, and its use contributes to
circular aquaculture. The aim of this study was to
produce acid and fermented silage from fish viscera to
determine their nutritional value and the apparent
digestibility coefficient of nutrients and energy for
juvenile tambaqui (Colossoma macropomum). An acid
silage and three fermented silages with different
sources of carbohydrates (molasses, wheat bran, and
cassava waste) were produced with 0.25\% sorbic acid as
an antifungal in the formulation. Silages presented an
average of 55\% dry matter, 62.9\% lipids, and 12\%
crude protein. Leucine and lysine predominated as
essential amino acids in the acid silage and fermented
silages. Fish viscera silages presented EPA and DHA
content from 5.4 to 17.8 and 1.7 to 8.9 mg.g {-1} of
lipids, respectively. The apparent digestibility
coefficient (ADC) (indirect method) was above 82\% for
gross energy for all the formulations. The ADCs for
protein were similar for the fermented silages, with a
maximum 92\% level for the fermented wheat bran silage.
The bioconversion of fish viscera into silage makes it
an energy ingredient for aquafeed that is well digested
by tambaqui juveniles.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Saliba:2023:DAM,
author = "Jaqueline Sim{\~o}es Saliba and Fabio Aremil Costa dos
Santos and Elo{\'\i}sa de Oliveira Sim{\~o}es Saliba
and Ronald Kennedy Luz",
title = "Different Animal Metabolism Markers for Artemia
Nauplii in Crude Protein Digestibility Assay for
\bioname{Lophiosilurus alexandri} Larvae",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "110",
day = "14",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020110",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/110",
abstract = "This work aimed to develop a methodology for marking
Artemia with different markers and evaluating their
passage rate and digestibility in Lophiosilurus
alexandri larvae of different ages. In the first phase,
Artemia nauplii were marked 28 h after hatching with
chromium oxide, titanium dioxide and NANOLIPE {\reg}.
Contact times with the markers were 30, 60, 90 min at a
concentration of 500 mg/L. Titanium dioxide had a
higher concentration in Artemia than the other markers.
The passage rate did not differ significantly between
titanium and NANOLIPE {\reg}. In the second phase, the
apparent digestibility of the protein in Artemia
nauplii was evaluated for L. alexandri larvae at three
ages. Average real protein digestibility at the three
ages was 95.08\%, demonstrating Artemia as a good
quality food. All ages exhibited the same final time of
fecal excretion of 30 min after feeding. Artemia
nauplii presented satisfactory results for assimilation
for all markers tested. In determining the
digestibility of Artemia protein, we can conclude that
chromium oxide and NANOLIPE {\reg} were good markers of
animal metabolism and titanium dioxide was the worst
marker of Artemia digestibility for the initial stages
of L. alexandri.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Romero:2023:HAC,
author = "Jaime Romero and Natalia Catal{\'a}n and Carolina
Ram{\'\i}rez and Claudio D. Miranda and Marcia Oliva
and H{\'e}ctor Flores and Mar{\'\i}a Soledad Romero and
Rodrigo Rojas",
title = "High Abundance of Candidatus Arthromitus in Intestinal
Microbiota of \bioname{Seriolella violacea} ({Palm
Ruff}) under Reared Conditions",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "109",
day = "13",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020109",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/109",
abstract = "Intestinal microbiota has been involved in several
processes that benefit the host, such as digestion,
nutrient metabolism, resistance to pathogens
colonization and immune function. In this study, we
investigated the diversity, composition and functional
prediction of microbiota of reared Seriolella violacea
(palm ruff) in the same cohort sampled at different
times (7-, 8- and 9-month-old). Microbial community
structure analyses, using 16S rRNA amplicon sequencing,
revealed that the intestinal microbiota was dominated
by the phyla Firmicutes, Proteobacteria, Fusobacteria
and Tenericutes. At the genus level, Candidatus
Arthromitus was the most abundant in all sampled
timepoints, representing in average 78\% of the
bacterial community (ranging from 18 to 98\%),
corresponding to segmented filamentous bacteria, which
are interesting because they have been associated with
the maturation of immune responses in the gut and
protecting the host from bacterial infections. The
comparisons of the intestinal microbiota among the
three groups showed differences in abundance of
bacterial taxa and also in alpha diversity indexes
(Shannon and Simpson), as well as beta diversity
metrics (weighted and unweighted UniFrac). Potential
functions of the intestinal microbiota of palm ruff
were retrieved using Philipin and Tax4fun and these
analyses revealed high levels of genes for sugar
metabolism. To our knowledge, this study represents the
first description of the intestinal microbiota of S.
violacea.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Du:2023:EMF,
author = "Ningning Du and Yanchun Sun and Zhongxiang Chen and
Xiaoli Huang and Chenhui Li and Lei Gao and Shuyan Bai
and Peng Wang and Qirui Hao",
title = "Effects of Multiple Freeze--Thaw Cycles on Protein and
Lipid Oxidation, Microstructure and Quality
Characteristics of Rainbow Trout (\bioname{Oncorhynchus
mykiss})",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "108",
day = "13",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020108",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/108",
abstract = "Multiple freeze-thaw cycles due to temperature
fluctuations irreversibly damage the muscle tissue of
fish, thereby reducing their edibility. The aim of this
study was to determine the effects of the number of
freeze-thaw (F--T) cycles on protein and lipid
oxidation, microstructure, physical index, and
nutritional quality of rainbow trout muscle. The
results showed that F--T cycles accelerated protein
carbonyl formation and thiobarbituric acid reactive
substances (TBARS) generation (p {$<$} 0.05), as well
as increased the loss of total sulfhydryl (SH) groups
(p {$<$} 0.05). Moreover, transmission electron
microscope (TEM) images illustrated that the
microstructure of muscle fibers was loosed and
disintegrated after the third F--T cycle, causing a
reduction in water holding capacity (WHC). In addition,
muscles lost the intrinsic color of fresh meat after
the fifth cycle, with lightness L* and yellowness b*
increasing, while redness a* declined (p {$<$} 0.05).
The hardness, springiness, and chewiness of muscles
decreased, and the shear force first increased and then
decreased after the third cycle. Furthermore, the
proximate components, essential amino acids (EAAs), and
total amino acids (TAAs), decreased significantly after
the third cycle (p {$<$} 0.05) due to the decrease of
WHC as well as protein and lipid oxidation. The results
indicated that the quality of rainbow trout muscle
changed after the third cycle, deteriorated seriously
after the fifth cycle, and was unacceptable after the
seventh cycle. Therefore, it is necessary to reduce the
temperature fluctuation to less than three times during
freezing. The results provided a reference for the
identification and classification of frozen aquatic
products.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhan:2023:GWI,
author = "Feifei Zhan and Liqun Liang and Shuangyi Wang and
Honjung Liew and Yumei Chang and Limin Zhang",
title = "Genome-Wide Identification, Phylogenetic Analysis and
Expression Pattern Profiling of the Aquaporin Family
Genes in \bioname{Leuciscus waleckii}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "107",
day = "11",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020107",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/107",
abstract = "Aquaporin (Aqp) is a transmembrane-specific channel
for small molecules that help in regulating homeostasis
in fishes when adapting to changing environments, but
its role in Amur ide's response to alkaline stress is
yet to be revealed. Therefore, the purpose of this
study is to investigate the response of the Aqp gene
exposed to alkaline water in Amur ide (Leuciscus
waleckii) using a genome-transcriptional assay. Based
on the results, we classified the Aqps of the L.
waleckii (LwAqps) genome and analyzed its
transcriptional expression profile and genetic
evolution under carbonate alkalinity stress. A total of
18 Aqp genes were identified in four grades in L.
waleckii. The highest Aqp gene expression was found in
the gill and kidney of L. waleckii from the Wusuli
River (WSL) in comparison to those in the Dali Lake
(DL), whereas aqp3a, -3ap1, -7, and -9a expressions
were found at intensively higher levels in the gill
rather than in the kidneys and livers. The experiment
of L. waleckii under alkalinity stress (carbonate
alkalinity 50 mmol{\cdot}L {-1}) and its recovery
showed that the expressions of aqp0a, -3a, -3ap1, -7,
-8aa, and -9a were upregulated in alkaline water and
downregulated in freshwater. We identified 1460 single
nucleotide polymorphism (SNP) markers in the Aqp genes.
The average value of Fst of SNP markers in the CDS
region was 0.177 {\pm} 0.256, and the first 5\% SNPs
were identified at aqp3a and -11b. Residue Ser66 does
not bring about an overall change in the
three-dimensional structure of Aqp3a, but may change
the penetration of solutes across the membrane. This
indicates that Aqp genes are involved in the response
of L. waleckii to alkaline stress, and aqp3a is one of
the key genes involved in regulating L. waleckii 's
adaptation to alkaline environments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lin:2023:RCP,
author = "Pengcheng Lin and Huaming Hu and Zheng Gong and Jian
Wang and Xin Gao",
title = "Reproductive Characteristics of \bioname{Pseudecheneis
sulcatus} ({Siluriforms: Sisoridae}) in the
\geoname{Lower Yarlung Zangbo River, Tibet}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "106",
day = "10",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020106",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/106",
abstract = "The present research offers novel understandings of
the reproductive biology of Pseudecheneis sulcatus
(McClelland, 1842) found in the Yarlung Zangbo Grand
Canyon, a biodiversity hotspot. Reproductive
characteristics of Pseudecheneis sulcatus, including
their maturation age, fertility, spawning season, and
maximum body size, constitute important aspects of its
life-history traits. We collected a total of 310
specimens from the Yarlung Zangbo River's Motuo reach
between March and November 2016 and found the
male-to-female ratio to be 0.73:1, markedly different
from the anticipated 1:1. The total length of
individuals ranged from 72 to 207 mm, while overall
weight ranged from 2.5 to 65.0 g. Their length at first
maturity was estimated as 131 mm in males and 132 mm in
females. Based on the adult proportion and fluctuation
in the monthly gonadosomatic index (GSI) and size
distribution of oocytes, spawning was determined to
occur from March to June. The absolute fecundity ranged
between 247 and 2886 eggs, while the fluctuation in
average relative fertility ranged between 16.8 and 77.1
eggs/g of fish. The fecundity of P. sulcatus was
linearly correlated to the overall weight and length of
the fish along with the ovarian weight. In conclusion,
P. sulcatus spawned synchronously in spring and early
summer, exhibiting low fecundity and large oocytes. Our
results provided basic information in understanding how
this species adapted well to the unique canyon
environment, which may be useful for developing a
sustainable conservation plan for P. sulcatus at the
lower reach of the Yarlung Zangbo River.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Eissa:2023:PSE,
author = "El-Sayed Hemdan Eissa and Ragaa A. Ahmed and Nadia A.
Abd Elghany and Amal Elfeky and Saadea Saadony and
Norhan H. Ahmed and Salah El-Sayed Sakr and Geraldine
B. Dayrit and Charlene Princess S. Tolenada and Adlene
Anne C. Atienza and Mahmoud Mabrok and Hala F. Ayoub",
title = "Potential Symbiotic Effects of $ \beta $-1,3 Glucan,
and Fructooligosaccharides on the Growth Performance,
Immune Response, Redox Status, and Resistance of
\geoname{Pacific} White Shrimp, \bioname{Litopenaeus
vannamei} to \bioname{Fusarium solani} Infection",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "105",
day = "10",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020105",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/105",
abstract = "The potential effects of dietary supplementation with
{\beta}-1,3 glucan and fructooligosaccharides
({\beta}-1,3 GF) on antioxidant activities,
immunological response, and growth performance of
Pacific white shrimp (Litopenaeus vannamei) was
investigated. Four diets (iso-energetic and
iso-nitrogenous) with different levels of {\beta}-1,3
GF (0, 0.5, 1.0, and 1.5 g kg {-1}) were fed to healthy
shrimp juveniles weighing 3 {\pm} 0.5 g for 75 days.
Shrimps were randomly distributed into 12 net
enclosures at a density of 30 shrimp/net, and the
experiment was performed in triplicate. The results
revealed that long-term supplementation with 1.5 g kg
{-1} {\beta}-1,3 GF significantly improved shrimp
weight gain, feed conversion ratio, and digestive
enzyme profiles compared to the control diet group.
However, there were no substantial variations in the
contents of moisture, crude protein, total lipids, and
ash in the muscles of shrimp fed on different diets.
Surprisingly, all antioxidants (superoxide dismutase,
catalase, glutathione peroxidase) and immune biomarkers
(lysozyme, total hemocyte count, phenol oxidase, and
respiratory burst) activities were significantly
elevated with increasing levels of {\beta}-1,3 GF in
the shrimp diet, and the highest values were recorded
in the 1.5 g kg {-1} diet groups. Challenge test
results revealed that F. solani could cause a high
mortality rate (86.7\%) in a group fed a normal basal
diet within 14 days at a dose of 5 $ \times $ 10 4
conidia mL {-1}. Surprisingly, all dietary treated
groups with different doses of {\beta}-1,3 GF showed
high resistance against F. solani, represented by lower
cumulative mortality rates (20--43.3\%) compared to the
control group. Moreover, most of the infected shrimp
showed a typical black to brown gill lesion similar to
that observed in the natural infection, where an
identical fungus was successfully re-isolated from
infected gills and muscles. Overall, this study
recommends an appropriate incorporation level of
{\beta}-1,3 GF that could enhance growth performance
and improve the antioxidant activities, non-specific
immunity, and disease resistance of L. vannamei, with
an optimal level of 1.5 g kg {-1}.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nenciu:2023:FDW,
author = "Magda Nenciu and Victor Ni{\c{t}}{\u{a}} and
Lumini{\c{t}}a Laz{\u{a}}r and Alina Sp{\^\i}nu and
Elena Vl{\u{a}}sceanu-Mateescu",
title = "Fostering the Development of \geoname{Western Black
Sea} Aquaculture: a Scientific Case Study for Finfish
Cage Farming Allocated Zone Designation",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "104",
day = "09",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020104",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/104",
abstract = "Mariculture offers enormous potential for providing
sustainable food, playing a key role in achieving
nutrition security, employment, and Blue Growth. This
is particularly true in geographical areas where the
dependence of local economies on fishery products is
high and yet access to sustainable landings is hindered
by environmental drawbacks. One such area is
represented by the Black Sea, which offers different
degrees of suitability for aquaculture development.
While the southern and eastern shores are sheltered
enough to allow for the development of large
aquaculture activities, the north-western shoreline is
characterized by wide environmental fluctuations and
the strong influence of the Danube. This study aimed at
investigating the suitability of a selected area of the
Romanian coast (Mangalia) for finfish cage farming by
adapting an internationally endorsed methodology for
determining its Degree of Compatibility (DC). The
development and expansion of finfish aquaculture
depends on the availability of space, so designating
Allocated Zones for Aquaculture (AZAs) is essential.
The result obtained (DC = 80) indicates that the
Mangalia area is suitable for finfish aquaculture
activities: there is no major interference with other
uses of the maritime space, no conflicts with nature
conservation, and the environmental conditions are
appropriate for fish culture in floating cages. The
novel information provided by this study can be the
building block for authorities to settle the governance
gap that has so far impeded the development of marine
aquaculture in Romania. At a larger scale, this study
can serve as a good practice example at the regional
Black Sea level.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Voorhees:2023:ECI,
author = "Jill M. Voorhees and Elizabeth R. J. M. Mamer and
Daniel J. Schill and Mitchel Adams and Carlos Martinez
and Michael E. Barnes",
title = "{17$ \beta $}-Estradiol Can Induce Sex Reversal in
Brown Trout",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "103",
day = "09",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020103",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/103",
abstract = "Hormones have been used to change phenotypic sex in
many fish species. However, information specific to
changing sex in brown trout Salmo trutta is lacking.
This study compared the effectiveness of two different
17{\beta}-estradiol (estradiol) concentrations (20
mg/kg and 30 mg/kg) fed to brown trout for 60 days,
beginning at initial feeding. At 456 days post-initial
feeding, the 20 mg/kg and 30 mg/kg treatment groups
were 84\% and 86\% female, respectively. These values
were significantly higher than the 47\% females
observed in the control group which did not receive
dietary estradiol. At the end of the 60-day estradiol
treatment period, weight gain, percent weight gain, and
feed conversion ratio were all significantly lower in
the tanks of fish receiving estradiol than in the
control tanks. Individual fish fed estradiol also
weighed significantly less and were significantly
shorter than the fish not receiving estradiol-coated
feed. Mortality ranged from 1.0 to 2.4\% among the
treatments and was not significantly different. After
105 days post-initial feeding, weight gain, percent
weight gain, and feed conversion ratio were not
significantly different among the treatments. At 456
days post-initial feeding, individual fish were
significantly longer and heavier in the 20 mg/kg
estradiol treatment compared to the fish in the control
treatment, and the fish in the 30 mg/kg estradiol
treatment were similar to the other two treatments.
This study is the first to document the successful sex
reversal of brown trout using estradiol. While the
estradiol treatments used in this study did not lead to
complete feminization, the observed 85-to-15\%
female-to-male phenotypic ratio indicates the
successful feminization of genetic males. The levels of
feminization observed in this study are suitable for
this initial step to potentially develop a YY male
broodstock to control invasive brown trout
populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dvoretsky:2023:SBO,
author = "Alexander G. Dvoretsky and Vladimir G. Dvoretsky",
title = "Shellfish as Biosensors in Online Monitoring of
Aquatic Ecosystems: a Review of \geoname{Russian}
Studies",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "102",
day = "08",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020102",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/102",
abstract = "The use of biological objects in monitoring the state
of the environment and the changes caused by the impact
of environmental pollution on marine and fresh waters
is a promising tool due to a lower cost in comparison
to traditional monitoring and the ability to receive
immediate information about the ecosystem status. In
this review, we summarize the biological information
about shellfish biomonitors and the results of studies
focused on the development and use of the bioindicator
species in early warning systems in Russia. Since the
mid-1980s, Russian specialists have developed online
biomonitoring systems; as in the rest of world, there
are two main approaches that are currently applied to
study the physiological status of potential biosensor
shellfish species and to monitor freshwater and marine
systems: valvometry (registration of gaping activity in
bivalve mollusks) and photoplethysmography
(registration of cardiac activity in mollusks and
crustaceans). Valve movement responses to stressors
such as abnormal conditions and pollutants include the
closure of shell valves for a long period, decrease in
the average distance between valves, rapid shell
opening, and higher closing frequency. Cardiac activity
reactions of shellfish to stress factors include rapid
increases in heart rate and stress index, higher
variability in heart rate than under normal conditions,
and longer periods required for heart rate recovery
after stress. The most common bioindicators used to
monitor environmental disturbances in marine ecosystems
are blue mussels, Iceland scallops, and red king crabs
in cold-water habitats and Black Sea mussels in warmer
waters as well as freshwater mussels and crayfish in
fresh waters.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kalan:2023:FFP,
author = "Parker Kalan and John Steinbeck and Freddy Otte and
Sean C. Lema and Crow White",
title = "Filter-Feeding \geoname{Pacific} Lamprey
(\bioname{Entosphenus tridentatus}) Ammocetes Can
Reduce Suspended Concentrations of \bioname{E. coli}
Bacteria",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "101",
day = "08",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020101",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/101",
abstract = "Filter-feeding invertebrates such as bivalves have
been shown to improve the health of aquatic systems by
reducing concentrations of bacteria and other harmful
suspended organisms, but it remains unknown if
microphagous suspension-feeding fishes can provide
similar ecosystem services for water quality. Here, we
tested whether the presence of the filter-feeding
larval ammocoete life-stage of Pacific lamprey
(Entosphenus tridentatus) can reduce suspended
concentrations of Escherichia coli bacteria. Aquaria
containing either filter-feeding ammocoete lamprey
larvae (1.5 fish{\cdot}L {-1}), lamprey macropthalmia
juveniles (1.5 fish{\cdot}L {-1}) that do not
suspension-feed, or no lamprey (control) were filled
with water contaminated with E. coli bacteria and then
monitored for 5 d for E. coli concentration changes in
the water column. The presence of ammocoete larvae
generated a significantly faster decline in E. coli
abundance compared to aquaria containing either
macropthalmia-stage lamprey or no fish, which showed
similar E. coli concentration profiles over that 5 d
period. A higher density of ammocoetes (4.3
fish{\cdot}L {-1}) resulted in a more rapid decline in
E. coli compared to the lower 1.5 fish{\cdot}L {-1}
ammocoete density, further implying that ammocoetes
augmented bacterial clearance. These observations
provide evidence that filter-feeding larval ammocoetes
of Pacific lamprey may help promote water-quality
enhancement by reducing suspended bacterial
concentrations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:ECV,
author = "Yang Wang and Fan Zhang and Zhe Geng and Yuying Zhang
and Jiangfeng Zhu and Xiaojie Dai",
title = "Effects of Climate Variability on Two Commercial Tuna
Species Abundance in the \geoname{Indian Ocean}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "99",
day = "07",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020099",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/99",
abstract = "Oceanic temperature fluctuations are one of the
leading factors affecting marine fish populations.
Indian Ocean Dipole (IOD), characterized as the sea
surface temperature (SST) anomaly change, is an
ocean--atmosphere interactive process causing
interannual climate variability in the Indian Ocean.
Influences of the IOD on the tuna catch rates are
supported by previous research. Yet, there remains
limited information about the impacts on the abundance
of tuna stocks. In this study, we used the standardized
Catch Per Unit Effort (CPUE) index to present the stock
abundance and compared the effects of the IOD on the
bigeye tuna (Thunnus obesus) and yellowfin tuna
(Thunnus albacares) among different management areas of
the Indian Ocean Tuna Commission (IOTC). Results show
significant correlations between IOD events on both
species' abundance in the tropical western Indian
Ocean. However, in the tropical eastern Indian Ocean
and the southern Indian Ocean, neither bigeye nor
yellowfin tuna abundances were significantly correlated
by the IOD. For the whole Indian Ocean, IOD was
significantly correlated uniquely with the yellowfin
tuna abundance. Our results emphasized the importance
of evaluating the climate variability effects over
fisheries abundance species by species and per fishing
areas analyses.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gao:2023:RSB,
author = "Tianheng Gao and Nannan Li and Wenlei Xue and Yuning
Hu and Hai Lin",
title = "The Responses of Sediment Bacterial Communities in
\geoname{Chinese} Mitten Crab (\bioname{Eriocheir
sinensis}) Culture Ponds to Changes in Physicochemical
Properties Caused by Sediment Improvement",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "98",
day = "07",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020098",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/98",
abstract = "The interaction between nutrients, heavy metals, and
sediment bacterial communities play a key role in the
health of crabs and the biogeochemical cycles of
aquaculture systems. However, the effects of sediment
improvement activities in crab culture on nutrients and
heavy metals and the response of bacterial communities
to the relevant changes are unclear. In this study, 24
water and sediment samples were collected from two
aquaculture sites (total of 12 ponds, 6 at each site).
High-throughput sequencing was used to determine the
structure of the bacterial community and the diversity
in water and sediment samples. The relationship between
nutrients, heavy metals, and bacterial communities and
the changes of the three before and after the
improvement of the sediment were analyzed. The results
showed that Proteobacteria, Bacteroidetes,
Acidobacteria, Chloroflexi, and Firmicutes were
predominant at the phylum level of sediment. Sediment
improvement has an effect on NH 4 + -N, sulfide, total
organic carbon (TOC), and heavy metals in sediments to
varying degrees. In addition, redundancy analysis found
that NH 4 + -N, NO 3 {-} -N, TP, and heavy metals were
key drivers in crab culture pond sediments. The results
of functional prediction showed that carbon, nitrogen,
and sulfur metabolism were the dominant processes in
the two crab farming areas. Overall, changes in
nutrients and heavy metals caused by sediment
improvement further affected the structure and function
of bacterial communities and may affect biogeochemical
cycles. Our study has deepened the understanding of the
effects of sediment improvement on nutrients, heavy
metals, and bacterial communities in crab culture
ponds.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Carlson:2023:BPD,
author = "Andrew K. Carlson and Mark V. Hoyer",
title = "Bluegill Population Demographics as Related to Abiotic
and Biotic Factors in \geoname{Florida} Lakes",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "100",
day = "07",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020100",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/100",
abstract = "Research on Bluegills, Lepomis macrochirus R., is
abundant but typically focuses on water bodies with
similar environmental conditions. We assessed Bluegill
density, relative abundance (catch per unit effort
[CPUE] by electrofishing), growth, and size structure
in 60 lakes with wide-ranging surface areas (2--12,412
ha), trophic states (oligotrophic--hypereutrophic), and
macrophyte abundances (0.3--100 percent of lake volume
inhabited [PVI]) across Florida, USA. Bluegill density
and CPUE increased with lake productivity and decreased
with macrophyte abundance. Bluegill growth increased
with lake productivity and CPUE of stock-length Florida
Bass, Micropterus floridanus L., a Bluegill predator.
Bluegill size structure increased with lake
productivity and decreased with Bluegill density.
Results indicate that Bluegill fisheries with abundant
individuals of quality size ({\geq}150 mm) require
productive ({$>$25} {\mu}g/L chlorophyll- a
concentration) lakes with moderate to high macrophyte
coverage (PVI 50--100), abundant stock-length Florida
Bass ({$>$40} fish/h of electrofishing), and Bluegill
densities {$<$300} fish/ha. This study provides an
approach to predict Bluegill population demographics
based on abiotic and biotic factors, establish
fisheries management expectations, and develop regional
and lake-specific management tools.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{DulceEstevao:2023:APR,
author = "Maria {Dulce Est{\^e}v{\~a}o}",
title = "Aquatic Pollutants: Risks, Consequences, Possible
Solutions and Novel Testing Approaches",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "97",
day = "06",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020097",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/97",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fantini-Hoag:2023:BAP,
author = "Leticia Fantini-Hoag and Terry Hanson and Jesse
Chappell",
title = "Bioeconomic Analysis of In-Pond Raceway System
Production of Foodsize and Stocker Hybrid Catfish
(Channel Catfish \bioname{Ictalurus punctatus}
[female-sign]$ \times $ Blue Catfish, \bioname{I.
furcatus} [male-sign])",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "96",
day = "06",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020096",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/96",
abstract = "The U.S. catfish industry is seeking production
systems that are efficient, intensive, and profitable.
Growing foodsize and stocker-sized fish in the same
pond is attractive as it is often difficult to obtain
larger-sized stockers early each year. This case study
evaluated the performance and economics of producing
foodsize hybrid catfish and stocker-sized fingerlings
in in-pond raceway systems (IPRS) placed into four 0.4
ha ponds. Growout raceways (RW1/RW2) in ponds 1 and 2
were 63 m 3, and 45 m 3 in ponds 3 and 4 (RW3/RW4).
Each pond had one (14 m 3) stocker unit raceway and a 5
HP of aeration that maintained adequate dissolved
oxygen levels. Average growout production was 12,050
kg/ha in 63 m 3 raceways and 12,078 kg/ha in 45 m 3
raceways (228 days of production) and achieved harvest
weights ranging from 564 to 661 g. The raceway stocker
unit had production yields ranging from 3537 to 4388
kg/ha (143 days of production) and achieved harvest
weights ranging from 123 to 234 g. Stocker units in
ponds 1 and 2 generated 8540 stocker--fingerlings
(21,102 fingerlings/ha) and units in ponds 3 and 4
generated 7954 fingerlings (19,654 fingerlings/ha). An
investment of USD 39,996 was needed for ponds 1 and 2
and USD 21,196 for ponds 3 and 4. When scenarios were
analyzed financially, positive financial net returns
occurred when farm level investment decreased, leading
to reduced payback periods, increased net present
values, and higher internal rates of return. IPRS used
stocker units to culture fingerlings for future
stocking of foodsize fish. IPRS provided good inventory
control, and high production yields compared to
traditional pond culture of catfish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Morales:2023:OMS,
author = "Christian James C. Morales and Kyle Dominic E.
Barnuevo and Emmanuel S. {Delloro, Jr.} and Roxanne A.
Cabebe-Barnuevo and Jenylle Kate S. Calizo and Sanny
David P. Lumayno and Ricardo P. Babaran",
title = "Otolith Morphometric and Shape Distinction of Three
Redfin Species under the Genus \bioname{Decapterus}
({Teleostei: Carangidae}) from \geoname{Sulu Sea,
Philippines}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "95",
day = "05",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020095",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/95",
abstract = "Otoliths are paired calcified structures in the inner
ear of teleosts that function in hearing and balance.
In this study, the use of otolith morphometrics and
shape analysis were explored to delineate the redfin
species under the genus Decapterus from the Sulu Sea,
Philippines, namely, Decapterus kurroides, D.
smithvanizi, and D. tabl. Results showed that the mean
otolith shapes are unique within species (p {$<$}
0.001). The otolith size-related (OW---otolith weight,
OL---otolith length, OH---otolith height, OA---otolith
area, and OP---otolith perimeter) and shape-related
(RE---rectangularity, SQ---squareness,
EL---ellipticity, RO---roundness, AR---aspect ratio,
FF---form factor, CO---compactness, and
CI---circularity) morphometric indices also showed
significant variations (p {$<$} 0.001) among the three
species. Distinct regions differentiating D. kurroides
from the other two species were seen along the dorsal
margin of the otoliths, which was supported by its
significantly higher values for OH (size-related index)
and RO (shape-related index). In addition, the
morphometric indices showed significant variations (p
{$<$} 0.001), except for RE. Furthermore, it was
revealed that the size-related indices were able to
separate D. kurroides from the other two species, which
turned out to be indifferent. The results provided
relevant data on the use of otoliths as an additional
confirmatory tool in species delineation of the
identified redfin species, and, therefore, offer
opportunities of applying the same method in
delineation and, eventually, identification of species
from other genera, especially those that closely
resemble each other.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhong:2023:TAF,
author = "Zhenxiao Zhong and Guozhu Chen and Haihui Tu and Xinyi
Yao and Xin Peng and Xuan Lan and Qiongying Tang and
Shaokui Yi and Zhenglong Xia and Miaoying Cai and
Guoliang Yang",
title = "Transcriptomic Analysis and Functional Gene Expression
in Different Stages of Gonadal Development of
\bioname{Macrobrachium rosenbergii}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "94",
day = "05",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020094",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/94",
abstract = "In order to decipher the functional genes and reveal
the molecular mechanism of gonadal development in
Macrobrachium rosenbergii, a comparative transcriptome
analysis was performed on the testes and ovaries at
different developmental stages. A total of 146,537
unigenes with an N50 of 2008 bp and an average length
of 1144 bp were obtained from the sequencing raw data
via quality control and denovo assembly. Identification
of differentially expressed genes (DEGs) showed that
there were 339 and 468 DEGs among the different
developmental stages of testes and ovaries,
respectively, and 7993 DEGs between the testes and
ovaries. The KEGG enrichment analysis identified 13
candidate pathways related to gonadal development,
including insulin synthesis, oocyte maturation, and
steroid biosynthesis, which were involved in biological
processes such as regulation of hormone metabolism, sex
cell proliferation and development, and amino acid
metabolism. The DEGs related to the above pathways such
as insulin-like growth factor 1 receptor (IGF1R), heat
shock protein 90 (Hsp 90), and cyclooxygenase (COX)
genes were highly expressed during yolk protein
synthesis, indicating that these genes might be
involved in yolk accumulation and oogenesis. Meanwhile,
calmodulin (CaM) and other genes were highly expressed
during spermatogenesis, suggesting that these genes
might play an important role in spermatogenesis. Ten
differentially expressed genes in the KEGG signaling
pathway, including CRQ, COX, APP, Cdc42, Hsd17b12,
Art-1, Hsp70, Hsp90, PRMT1, and GP, were selected for
real-time quantitative PCR (RT- qPCR) to validate the
transcriptome data, and the results showed that RT-
qPCR obtained consistent results with the RNA-Seq data.
The present findings provide new insights into the
molecular regulation mechanism of gonadal development
in M. rosenbergii.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mokhtar:2023:MCF,
author = "Doaa M. Mokhtar and Giacomo Zaccone and Alessio Alesci
and Michal Kuciel and Manal T. Hussein and Ramy K. A.
Sayed",
title = "Main Components of Fish Immunity: an Overview of the
Fish Immune System",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "93",
day = "05",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020093",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/93",
abstract = "Cellular immune responses consist of innate and
adaptive cell-mediated immune mechanisms, where all
leukocyte subpopulations are included. Among these are
vital processes such as cell-mediated cytotoxicity and
phagocytosis. The main cellular constituents of the
fish immune system are macrophages, granulocytes,
dendritic cells, NK cells, and cytotoxic T cells. This
review provides the latest information on cellular
defense mechanisms of fish and provides an overview of
the function of the mucosal immune system in
maintaining the general health of fish. Here, we
discuss the fundamental ideas that underpin mucosal
immune responses in teleosts, as well as the innate and
adaptive immune cells and the molecules that play a
role in these immune responses. Moreover, cytokine
molecules and pathways in teleosts have been reported
to focus on several kinds of associated immunity.
Importantly, we also review antigen processing and
presentation. The knowledge reported here will enable
better understanding, determination, and modulation of
the pathways responsible for protective immunity, thus
consequently improving the health of the fish in
aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2023:TMA,
author = "Guangwei Hu and Weili Wang and Kai Xu and Chao Wang
and Dexue Liu and Jing Xu and Binlun Yan and Nanjing Ji
and Huan Gao",
title = "Transcriptomic and Metabolomic Analyses of
\bioname{Palaemon carinicauda} Hepatopancreas in
Response to \bioname{Enterocytozoon hepatopenaei}
({EHP}) Infection",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "92",
day = "05",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020092",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/92",
abstract = "Enterocytozoon hepatopenaei (EHP), a microsporidian
responsible for hepatopancreatic microsporidiosis, is a
major pathogen in commercial shrimp production. Among
the affected species, Palaemon carinicauda (formerly
Exopalaemon carinicauda) is commercially important in
China and represents a potential research model for
studying crustaceans. However, little information is
available on its response to EHP infection. Hence, this
study analyzed the transcriptome and metabolome of P.
carinicauda 's hepatopancreas using high-throughput
sequencing and liquid chromatograph-mass spectrometry
(LC-MS) to determine its response during the early
stage of infection. The transcriptomic analyses
identified 730 differentially expressed genes, of which
those associated with EHP infection were enriched in
metabolic pathways as well as detoxification and
antioxidant pathways. In addition, 144 differential
metabolites were identified using a combination of
positive and negative ion modes in LC-MS. The Kyoto
encyclopedia of genes and genomes pathway analyses
further indicated that the degradation of aromatic
compounds, the AMP-activated protein kinase signaling
pathway and C5-branched dibasic acid metabolism were
significantly enriched after EHP infection. These
results could provide useful insights into the effects
of EHP on shrimps during the early stages of infection
and help to understand the mechanisms underlying the
stunted growth of shrimps after infection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Filipe:2023:SSF,
author = "Diogo Filipe and M{\'a}rio Dias and Rui Magalh{\~a}es
and Helena Fernandes and Jos{\'e} Salgado and Isabel
Belo and Aires Oliva-Teles and Helena Peres",
title = "Solid-State Fermentation of Distiller's Dried Grains
with Solubles Improves Digestibility for
\geoname{European} Seabass (\bioname{Dicentrarchus
labrax}) Juveniles",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "90",
day = "03",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020090",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/90",
abstract = "Aquaculture requires new, economical, and eco-friendly
protein sources to replace traditional fisheries and
plant ingredients. Using agriculture by-products as
protein sources would reduce land-based feed production
pressure and waste production, promoting a circular
economy and sustainable aquaculture. Distiller's dried
grains with solubles (DDGS) is the main by-product of
bioethanol production. Corn DDGS has a high protein
level, but its high fiber content limits its use as a
feed ingredient, particularly for carnivorous fish.
Solid-state fermentation (SSF) uses
lignocellulosic-rich substrates, such as DDGS, for
microbial growth in the near absence of water,
promoting enzyme production that degrades the
lignocellulosic matrix, increasing free reducing
sugars, protein, and antioxidant levels of the
substrate. In the present work, the SSF of corn DDGS
with Aspergillus carbonarius, A. ibericus, and A.
uvarum was tested. Then, the digestibility of the most
promising fermented DDGS (in terms of upgraded
nutritional composition) was tested by including it in
a reference diet (70\% of a reference diet; 48\% crude
protein; 15\% crude lipids) for European seabass
(Dicentrarchus labrax) juveniles (171 g averaged
weight; trial duration of 52 days). Among the fungi
tested, Aspergillus ibericus led a generally higher
upgrading of the DDGS nutritional composition, leading
to a high amount of protein (from 42.7 to 49.7 g N/kg
DM), phenolic compounds (1.49 to 4.86 mg/g caffeic acid
equivalents), free sugars (9.5 to 31.9 mg/g), and
enzyme production (45 U/g and 68 U/g of cellulase and
xylanase, respectively), and a high reduction in acid
detergent fiber and neutral detergent fiber content (up
to 29 and 43\%, respectively). Compared to the
unfermented DDGS, fermented DDGS presented increased
protein, lipids, starch, and energy digestibility,
while phosphorous digestibility was similar. Compared
to the reference diet, dietary inclusion of unfermented
or fermented DDGS increased trypsin and chymotrypsin
activities. The activity of digestive enzymes was not
affected by the inclusion of fermented DDGS, except for
amylase activity, which was lower with the fermented
DDGS than with the unfermented DDGS diet. In
conclusion, SSF of DDGS enhanced its nutritional value,
increasing DDGS digestibility when included in diets
for European seabass juveniles.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wei:2023:GSP,
author = "Xuelian Wei and Zeqin Fu and Jiji Li and Baoying Guo
and Yingying Ye",
title = "Genetic Structure and Phylogeography of Commercial
\bioname{Mytilus unguiculatus} in \geoname{China} Based
on Mitochondrial \gene{COI} and \gene{Cytb} Sequences",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "89",
day = "02",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020089",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/89",
abstract = "In order to study the genetic structure and population
geographic distribution pattern of coastal mussel
populations in the coast of China, mitochondrial DNA
(COI and Cytb genes) were used to analyze the genetic
diversity, genetic structure, and population history
dynamics of Mytilus unguiculatus in the East China Sea
and the Yellow Sea. We detected high levels of genetic
diversity in seven populations of M. unguiculatus. A
total of 34 haplotypes of COI genes and 29 haplotypes
of Cytb were obtained. The haplotype diversity of COI
ranged from around 0.77 to 0.93 (Cytb: 0.83--0.91). The
nucleotide diversity of COI ranged from around 0.0044
to 0.0064 (Cytb: 0.0049--0.0063). The coefficient of
genetic differentiation (F ST) of COI ranged from
around 0.031 to 0.080, and Cytb ranged from around
0.028 to 0.039. Analysis of molecular variance (AMOVA)
and a phylogenetic tree showed that the genetic
structure was relatively weak, and there was no clear
population differentiation. The neutrality test results
showed that Tajima's D value and Fu's Fs value were not
significant, and no significant population demographic
events, including population expansion or population
bottleneck, were detected in the samples. The Bayesian
skyline graph analysis showed that the effective
population size has been relatively stable for nearly
10,000 years, without any large population
fluctuations. It was speculated that the seven
populations in the present study should belong to the
same group. This study provides a comprehensive survey
of the genetic characteristics of M. unguiculatus,
filling the gaps among related studies. It provides
theoretical support and material accumulation for seed
selection and breeding, genetic resources' protection,
and breeding management in the future.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kerzerho:2023:MRA,
author = "Vincent Kerz{\'e}rho and Florence Aza{\"\i}s and Serge
Bernard and Sylvain Bonhommeau and Blandine Brisset and
Laurent De Knyff and Mohan Julien and Michel Renovell
and Tristan Rouyer and Claire Saraux and Fabien
Soulier",
title = "Multilinear Regression Analysis between Local
Bioimpedance Spectroscopy and Fish Morphological
Parameters",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "88",
day = "01",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020088",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/88",
abstract = "Repeated fish handling may cause stress, which biases
experiments and so affects the results. In order to
reduce this, the present study investigates the benefit
of using bioimpedance analysis to estimate
morphological parameters. Bioimpedance analysis is a
non-lethal and integrative electrical measurement that
can be used to estimate several kinds of physiological
parameters and is used in medicine and ecological
studies. In particular, bioimpedance can monitor the
individual growth of fish, which is a prerequisite for
most biological and ecological studies, as body size is
one of the best predictors for numerous life history
traits and ecological parameters. After a short review
on the use of bioimpedance measurement in medicine and
ecology, we illustrate the potential of bioimpedance
spectroscopy, as opposed to single frequency
measurement, for experimental studies on fish. Using a
monolithic four-contact electrode and a cost-effective
portable system, we conducted bioimpedance spectroscopy
on 159 sardines. The association between the length,
weight, and electrical parameters obtained at different
frequencies from the bioimpedance spectroscopy was
investigated. Our results show that accounting for more
than one frequency substantially improves the
prediction of length and weight. We conclude that
bioimpedance could potentially be a powerful tool for
monitoring fish growth in ecological studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Duchatelet:2023:ECP,
author = "Laurent Duchatelet and Charlotte Nuyt and Nathan
Puozzo and J{\'e}r{\^o}me Mallefet and J{\'e}r{\^o}me
Delroisse",
title = "Evolutionary Conservation of Photophore Ultrastructure
in Sharks: The Case of a Dalatiid Squalomorph",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "87",
day = "01",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020087",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/87",
abstract = "Bioluminescence is a common ecological trait among
many marine organisms, including three shark families:
Etmopteridae, Dalatiidae, and Somniosidae. The kitefin
shark, Dalatias licha (Bonnaterre, 1788), from the
Dalatiidae family is the largest known luminous
vertebrate. This study compares the light organ
ultrastructure of D. licha with that of Etmopterus
spinax, the type species of Etmopteridae, to gain a
deeper understanding of the light emission process and
its evolutionary conservation within shark families.
The ultrastructure of D. licha 's photophores and the
morphological changes that occur after hormonal
stimulation (via melatonin and \alpha -MSH, which
stimulate or inhibit the bioluminescence, respectively)
were examined. The photophores consist of a spherical
pigmented sheath surrounding a unique, regionalized
light-emitting cell (photocyte). The photocyte's basal
area contains a specific area filled with granular
inclusions that resemble the glowon-type microsources
of E. spinax, suggesting that this area is the
intracellular site of light emission. An acidophilic
secretion, not present in Etmopteridae, is also
observed within the granular area and may be involved
in photogenesis. The ultrastructure analysis reveals no
lens cells or reticular layer, unlike in Etmopteridae
photophores, indicating a simpler organization in
Dalatiidae photophores. Melatonin stimulation causes
the removal of pigments from the photophore-associated
melanophores and an increase in the granular inclusion
diameter and coverage in the granular area, further
showing that this last area is the potential site of
light emission, while \alpha -MSH stimulation causes
the extension of the melanophore pigments and a
decrease in the granular inclusion diameter and
coverage. These results support the evolutive
conservation of photophore functional organization
across luminous etmopterid and dalatiid sharks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shi:2023:STV,
author = "Yongchuang Shi and Bo Kang and Wei Fan and Lingling Xu
and Shengmao Zhang and Xuesen Cui and Yang Dai",
title = "Spatio-Temporal Variations in the Potential Habitat
Distribution of \geoname{Pacific} Sardine
(\bioname{Sardinops sagax}) in the \geoname{Northwest
Pacific Ocean}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "86",
day = "31",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020086",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/86",
abstract = "Pacific sardine (Sardinops saga x) is a commercially
important species and supports important fisheries in
the Northwest Pacific Ocean (NPO). Understanding the
habitat distribution patterns of Pacific sardine is of
great significance for fishing ground prediction and
stock management. In this study, both single-algorithm
and ensemble distribution models were established
through the Biomod2 package for Pacific sardine by
combining the species occurrence data, sea surface
temperature (SST), sea surface height (SSH), sea
surface salinity (SSS) and chlorophyll-a concentration
(Chla) in the NPO during the main fishing season
(June--November) from 2015 to 2020. The results
indicated that the key environmental variables
affecting the habitat distribution of Pacific sardine
were the SSH and SST. The suitable habitat area for
Pacific sardine showed significant monthly changes: the
suitable habitat range in June was larger than that in
July and August, while the suitable habitat range
gradually increased from September to November.
Furthermore, the monthly geometric centers of habitat
suitability index (HSI) for Pacific sardine presented a
counterclockwise pattern, gradually moving to the
northeast from June, and then turning back to the
southwest from August. Compared with single-algorithm
models, the ensemble model had higher evaluation metric
values and better spatial correspondence between
habitat prediction and occurrence records data, which
indicated that the ensemble model can provide more
accurate prediction and is a promising tool for
potential habitat forecasting and resource
management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barash:2023:DPD,
author = "Adi Barash and Aviad Scheinin and Eyal Bigal and Ziv
Zemah Shamir and Stephane Martinez and Dan Tchernov",
title = "Depth Partitioning and Diel Movement of Two Large
Carcharhinid Sharks in Extremely Shallow Waters",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "85",
day = "31",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020085",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/85",
abstract = "Two species of carcharhinid sharks aggregate every
winter at the warm water effluent of a coastal power
plant on the Israeli Mediterranean coast. The two
species (Carcharhinus obscurus and Carcharhinus
plumbeus) cooccur in a highly confined area for several
months every year and are highly associated with the
area in and around the hot water effluent. Niche
partitioning has recently been suggested as a mechanism
that enables the coexistence of similar shark species
by resource partitioning, spatial partitioning, and
temporal partitioning. In this study, we used acoustic
telemetry to study the individual diel movement and
activity patterns within this enclosed area and
examined the differences between the two species
sharing it. Although this location only reaches a
maximum depth of 7.5 m, we found both species perform a
diel vertical movement, rising closer to the surface at
night and moving deeper during daytime. Furthermore,
the two shark species swam at different depths both day
and night, with C. obscurus swimming in the upper
column, about 2 m shallower than C. plumbeus. The very
small scale of movement, which nearly equals the
sharks' body length, suggests movement patterns might
be conserved at the species level. Moreover,
spatiotemporal differences between the two species may
reflect a mean of interspecific partitioning that
occurs even in a highly confined and shallow habitat.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jiao:2023:PST,
author = "Jinbiao Jiao and Jing Chen and Jiayun Yao and Yanli Li
and Xuemei Yuan and Lei Huang and Shengqi Su and Haiqi
Zhang",
title = "The Pathology and Splenic Transcriptome Profiling of
\bioname{Trionyx sinensis} Challenged with
\bioname{Bacillus cereus}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "84",
day = "31",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020084",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/84",
abstract = "The pathogenic Bacillus cereus strain XS0724 isolated
from China can cause high lethality to Trionyx
sinensis, but little information is available on their
detailed interactions. In this study, histopathologic
profiling indicated that B. cereus caused vacuolization
and cell necrosis in the liver, spleen, kidney, and
intestine. The identification of the virulence factor
genes non-hemolytic enterotoxin (Nhe), hemolysin BL
(Hbl), and enterotoxin FM (entFM) confirmed bacterial
pathogenicity. Splenic transcriptomic sequencing at 96
h post-infection identified various immune-related
genes mapped to diverse gene families, including
interleukin, complement, chemokine, and interferon. The
differentially expressed genes (DEGs) were enriched in
2174 GO terms: 1694 in biological processes, 138 in
cellular components, and 342 in molecular functions.
Further KEGG enrichment indicated that DEGs were
primarily associated with the phagosome, NF-kappa B
signaling pathway, and PI3K-Akt signaling pathway. The
DEGs and enriched pathways may be involved in the
elimination of invasive B. cereus. These data laid the
foundation for elucidating the potential molecular
mechanisms in this bacterial infection process, and
provided robust genetic evidence for subsequent work on
resistance genes of T. sinensis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Imsland:2023:CFA,
author = "Albert Kjartan Dagbjartarson Imsland",
title = "Cleaner Fish in Aquaculture",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "83",
day = "31",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020083",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/83",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Silkin:2023:TPE,
author = "Yuriy A. Silkin and Mikhail Yu. Silkin and Elizaveta
N. Silkina and Alla Silkina",
title = "Thermal Phenomena in Erythrocytes of the
\geoname{Black Sea} Thornback Ray (\bioname{Raja
clavata} {L.}) and Black Scorpionfish
(\bioname{Scorpaena porcus} {L.}) In Vitro",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "82",
day = "31",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020082",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/82",
abstract = "An evolutionary development of the rheological
characteristics of blood in the capillary blood flow is
one of indicator of fishes' adaptation for the changing
temperature conditions. Our earlier studies showed that
large, nucleated erythrocytes of the Black Sea
thornback ray (Raja clavata L.) and scorpionfish
(Scorpaena porcus L.) had high ecto-ATPase activity.
This activity varied from 3.0 to 3.9 and 6.0 to 10.0
nmol Fn/min/$ \mu $L RBC in rays and scorpionfish,
respectively. The use of thermal energy of ATP in the
hydrolysis process made by ecto-ATPases in fish
erythrocytes provided a heating of the plasma cells'
membrane and the near-wall plasma layer with an aim to
improve the rheological characteristics of blood in the
capillary blood flow. To confirm the proposed
assumption, we studied thermal phenomena in
erythrocytes suspensions of fishes by continuous
recording of temperature changes in the experimental
cell with fishes' erythrocytes suspension with the
addition of ATP (1 mg/mL) in vitro. A higher
``thermal'' activity of scorpionfish erythrocytes was
established in comparison with erythrocytes of the
thornback ray, which had the multiplicity of activity
of ecto-ATPase in the plasma membranes of erythrocytes
of the studied fishes. The presence of heat-producing
activity of suspensions of erythrocytes of thornback
ray and scorpionfish is evidence of the possible use of
thermal energy of ATP hydrolysis by fish erythrocytes,
both to maintain the functional state of the plasma
membrane of red blood cells and to improve the
rheological characteristics of blood in the capillary
section of blood flow.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nuswantoro:2023:EPE,
author = "Soko Nuswantoro and Tzu-Yuan Sung and Meki Kurniawan
and Tsung-Meng Wu and Bonien Chen and Ming-Chang Hong",
title = "Effects of Phosphate-Enriched Nutrient in the
Polyculture of \geoname{Nile} Tilapia and Freshwater
Prawn in an Aquaponic System",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "81",
day = "30",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020081",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/81",
abstract = "Aquaponic systems are made up of hydroponic beds and
recycled aquaculture systems. The significant elements
that determine how effectively an aquaponic system
operates are the nitrogen cycle (nitrification) and the
phosphorus cycle (phosphate). Because some research
indicates that aquaponics systems are primarily
deficient in phosphorus, phosphate-enriched nutrients
were added to raise the phosphorus levels. During an
eight-week experimental period, the effects of water
quality parameters and microbiology, animal and plant
growth performance, chlorophyll compounds in lettuce,
and the bacterial community were analyzed. Phosphate
concentration (1.604 \pm 1.933 mg L \minus 1) and
ammonia-oxidizing bacteria (AOB) (1.19 $ \times $ 10 2
\pm 1.30 CFU mL \minus 1) give significant positive
reactions to the added nutrients. However, the prawn
survival rate (17.00 \pm 0.63\%) showed a significantly
negative response to nutrition modification containing
phosphate, and the percentage of bacterial pathogens
became more dominant (pathogen 40.51\%; N_bacteria
35.05\%; probiotics 24.44\%). This study shows that
adding phosphate increases phosphorus levels in an
aquaponics system and changes the microbial community
and species growth performance.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cai:2023:SAC,
author = "Kai Cai and Richard Kindong and Qiuyun Ma and Siquan
Tian",
title = "Stock Assessment of Chub Mackerel (\bioname{Scomber
japonicus}) in the \geoname{Northwest Pacific} Using a
Multi-Model Approach",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "80",
day = "30",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020080",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/80",
abstract = "Chub mackerel (Scomber japonicus) is a major targeted
species in the Northwest Pacific Ocean, fished by
China, Japan, and Russia, and predominantly captured
with purse seine fishing gear. A formal stock
assessment of Chub mackerel in the region has yet to be
implemented by the managing authority, that is, the
North Pacific Fisheries Commission (NPFC). This study
aims to provide a wider choice of potential models for
the stock assessment of Chub mackerel in the Northwest
Pacific using available data provided by members of the
NPFC. The five models tested in the present study are
CMSY, BSM, SPiCT, JABBA, and JABBA-Select. Furthermore,
the influence of different data types and input
parameters on the performance of the different models
used was evaluated. These effects for each model are
catch time series for CMSY, catch time series and prior
of the relative biomass for BSM, prior information for
SPiCT, and selectivity coefficients for JABBA-Select.
Catch and CPUE (catch per unit effort) data used are
derived from NPFC, while some life history information
is referred from other references. The results indicate
that Chub mackerel stock might be slightly overfished,
as indicated by CMSY (B 2020 / B MSY = 0.98, F 2020 / F
MSY = 1.12), BSM (B 2020 / B MSY = 0.97, F 2020 / F MSY
= 1.21), and the base case run for the JABBA-Select (SB
2020 / SB MSY = 0.99, H 2020 / H MSY = 0.99) models.
The results of the models SPiCT (B 2020 / B MSY = 2.30,
F 2020 / F MSY = 0.31) and JABBA (B 2020 / B MSY =
1.40, F 2020 / F MSY = 0.62) showed that the state of
this stock may be healthy. Changes in the catch time
series did not affect CMSY results but did affect BSM.
The present study confirms that prior information for
BSM and SPiCT models is very important in order to
obtain reliable results on the stock status. The
results of JABBA-Select showed that different
selectivity coefficients can affect the stock status of
a species, as observed in the present study. Based on
the optimistic stock status indicated by the best
model, JABBA, a higher catch is allowable, but further
projection is required for specific catch limit
setting. Results suggested that, as a precautionary
measure, management would be directed towards
maintaining or slightly reducing the fishing effort for
the sustainable harvest of this fish stock, while
laying more emphasis on accurately estimating prior
input parameters for use in assessment models.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:QMG,
author = "Yuhan Chen and Jintai Huang and Zhan Jin and Junping
Chen and Meng Zhang and Miao Yu and Hongxia Jiang and
Lei Wang and Zhigang Qiao",
title = "{QTL} Mapping of Growth Traits in \geoname{Yellow
River} Carp (\bioname{Cyprinus carpio haematopterus})
at 5--17 Months after Hatching",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "79",
day = "29",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020079",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/79",
abstract = "To screen the quantitative trait loci (QTL) and genes
related to the growth of Yellow River carp (Cyprinus
carpio haematopterus) after removing the maternal
effect, we established a family of these carps. Four
growth-related traits (body length, height, thickness,
and weight) of the family at 5 and 17 months after
hatching (MAH) were measured. Analysis of QTL mapping
for the four growth-related traits was conducted using
the genetic linkage map constructed in our laboratory.
We identified 47 QTL that were related to the four
growth traits and three consensus QTL (cQTL). A total
of 10, 14, 10, and 13 QTL were associated with body
length, height, thickness, and weight, respectively;
cQTL-1, cQTL-2, and cQTL-3 contained 11, 2, and 2 QTL,
respectively. We detected 17 growth-related candidate
genes within 50 Kb upstream and downstream of the five
main-effect QTL (phenotypic variation explained {$>$}
10\%, logarithm of odds {$>$} 5.5). Two genes (cbfa2t2
and neca1) that may be affected by maternal effects
were identified by comparing the main-effect QTL at 17
and 5--17 MAH. This study was the first attempt to
eliminate growth-related QTL and genes affected by
maternal effects in Yellow River carp. These results
can be used in molecular marker-assisted breeding and
provide valuable genomic resources for the genetic
mechanisms underlying growth in Yellow River carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2023:ENT,
author = "Shenglong Yang and Linlin Yu and Fei Wang and Tianfei
Chen and Yingjie Fei and Shengmao Zhang and Wei Fan",
title = "The Environmental Niche of the Tuna Purse Seine Fleet
in the \geoname{Western and Central Pacific Ocean}
Based on Different Fisheries Data",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "78",
day = "29",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020078",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/78",
abstract = "Understanding the spatial pattern of human fishing
activity is very important for fisheries resource
monitoring and spatial management. The environmental
preferences of tropical tuna purse seine fleet in the
Western and Central Pacific Ocean (WCPO) were
constructed and compared at different spatial scales
based on the fishing effort (FE) data from the
available automatic identification system (AIS) and
commercial fishery data compiled from the Western and
Central Pacific Fisheries Commission (WCPFC), using
maximum entropy (MaxEnt) methods. The MaxEnt models
were fitted with FE and commercial fishery data and
remote sensing environmental data. Our results showed
that the area under the curve (AUC) value each month
based on the commercial fishery data (1\degree) and FE
at 0.25\degree and 0.5\degree spatial scales was
greater than 0.8. The AUC values each month based on
the FE data at a 1\degree scale ranged from 0.775 to
0.829. The AUC values based on commercial fishing data
at the 1\degree scale were comparable to the model
results based on FE data at the 0.5\degree scale and
inferior to the model results based on FE data at the
0.25\degree scales. Overall, the sea surface
temperature (SST), temperature at 100 metres (T100),
oxygen concentration at 100 metres (O100) and total
primary production (PP) had the greatest influence on
the distribution of the purse seine tuna fleet. The
oxygen concentration at 200 metres (O200), distance to
shore (DSH), dissolved oxygen (Dox), EKE, mixed layer
depth (Mld), sea surface salinity (SSS), salinity at
100 metres (S100) and salinity at 200 metres (S200) had
moderate influences, and other environmental variables
had little influence. The suitable habitat areas varied
in response to environmental conditions. The purse
seine tuna fleet was mostly present at locations where
the SST, T100, O100, O200 and PP were 28--30 \degree C,
27--29 \degree C, 150--200 mmol/m 3 and 5--10 mg/m
\minus 3, respectively. The MaxEnt models enable the
integration of AIS data and high-resolution
environmental data from satellite remote sensing to
describe the spatiotemporal distribution of the tuna
purse seine fishery and the influence of environmental
variables on the distribution, and can provide
forecasts for fishing ground distributions based on
future remote sensing environmental data.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Choi:2023:PEE,
author = "Young Jae Choi and Seul Gi Na Ra Park and A-Hyun Jo
and Jun-Hwan Kim",
title = "Physiological Effect of Extended Photoperiod and Green
Wavelength on the Pituitary Hormone, Sex Hormone and
Stress Response in Chub Mackerel, \geoname{Scomber
japonicus}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "77",
day = "29",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020077",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Choi:2023:CCA}.",
URL = "https://www.mdpi.com/2410-3888/8/2/77",
abstract = "Chub mackerel, Scomber japonicus, is heavily farmed
and harvested due to its demand as a high-quality
protein source rich in fatty acids. However, the
effects of environmental cues on sexual maturation of
the fish remain understudied. We aim to elucidate the
effect of light manipulation on the hormones related to
reproduction and on the stress response in the species.
Mackerel were exposed to different photoperiods (12 h
light:12 h dark or 14 h light:10 h dark) and light
wavelengths (provided by white fluorescent bulbs or
green LEDs). Total RNA extracted from the brain was
assayed with quantitative polymerase chain reaction (a
powerful technique for advancing functional genomics)
and blood plasma was analyzed via immunoassay using
ELISA kits. The mRNA expression of gene-encoding
gonadotropin-releasing hormone, gonadotropin hormone,
follicle-stimulating hormone, and luteinizing hormone
were significantly increased through the use of an
extended photoperiod and green wavelength, which also
increased testosterone and 17\beta -estradiol plasma
levels. Plasma levels of cortisol and glucose, which
are indicators of a stress response, were significantly
decreased through green LED exposure. Our results
indicate that environmental light conditions affect the
production of pituitary and sex hormones, and reduce
the stress response in S. japonicus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:NDD,
author = "Yaxi Li and Zhi Yan and Ainuo Lin and Xiaodong Li and
Ke Li",
title = "Non-Dose-Dependent Relationship between Antipredator
Behavior and Conspecific Alarm Substance in Zebrafish",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "76",
day = "28",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020076",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/76",
abstract = "A series of behavioral detection paradigms have been
developed for zebrafish (Danio rerio) to examine
anxiety-like behavioral responses. Among them, the
novel tank diving test is rapidly gaining popularity in
translational neuroscience and behavioral research for
the investigation of psychopharmacological activity
focusing on stress. Zebrafish respond to conspecific
epidermal-released alarm substances with antipredator
reactions. Although the alarm responses of zebrafish
were well characterized in a novel tank diving
experiment, the relationship between the intensity of
the alarm behavior and the concentration of the alarm
substance needed to be understood more adequately. In
the current paper, we investigated the behavioral
phenotypes and potencies of zebrafish elicited by the
serial dilution of an alarm substance in the novel tank
diving test. Using a video-tracking assisted behavioral
quantification approach, we demonstrated no linear
concentration-dependent relationship between
antipredator behavior and skin extracts, suggesting
that an optimal concentration induced each typical
behavioral response. The results showed that the
freezing duration (\%) significantly increased when
stimulated with 10 4 -fold times dilutions of skin
extract (equivalent 5 $ \times $ 10 \minus 5 fish/L),
while erratic movements (\%), time in the bottom half
(\%), and latency to the upper half (s) significantly
elevated when stimulated with 10 3 -fold times
dilutions (equivalent 5 $ \times $ 10 \minus 4 fish/L).
Therefore, the concentration threshold for an alarm
substance that elicited innate fear behavior in
zebrafish was presumed to be an equivalent
concentration of approximately 5 $ \times $ 10 \minus 4
fish/L. The conclusions may fill a knowledge gap
between the innate fear response triggered by injured
skin and a novel tank diving paradigm that provides
insights into the characterization of alarm substance,
behavioral responses, and physiological response
mechanisms in zebrafish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luo:2023:GDJ,
author = "Yang Luo and Yufeng Zhang and Ruli Cheng and Qinghua
Li and Yu Zhang and Yingwen Li and Yanjun Shen",
title = "Genetic Diversity of \bioname{Jinshaia sinensis}
({Cypriniformes, Balitoridae}) Distributed Upstream of
the \geoname{Yangtze River}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "75",
day = "28",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020075",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/75",
abstract = "The upper reaches of the Yangtze River (upper YR) are
a biological zone with extremely rich fish diversity,
especially endemic fish. However, long-term human
interference, such as environmental pollution and
cascade hydropower construction, has significantly
changed the habitat of many fish and is threatening the
number and genetic diversity of fish populations.
Jinshaia sinensis is a typical small and endemic but
rare fish that is found in the upper YR, and its
genetic diversity and structure still need further
study. To understand the current levels of genetic
diversity in J. sinensis, we analyzed the genetic
diversity, population history, genetic structure, etc.,
of three J. sinensis populations based on two
mitochondrial genes (the cytochrome-c oxidase subunit
I, COI, and cytochrome-b gene, Cytb) and two nuclear
genes (recombination-activating protein 1, RAG1, and
rhodopsin, RH). The genetic diversity analysis
indicated that J. sinensis had high genetic diversity,
with high haplotype diversity (h) and nucleotide
diversity (Pi). Population pairwise F ST analysis
revealed a significant genetic divergence between the
Lijiang and Luzhou populations for all genes and
between the Panzhihua and Luzhou populations, except
for the COI gene; however, analyses of molecular
variance (AMOVA) showed no significant geographic
genetic structure among populations, and gene flow
analysis also indicated a certain degree of gene
exchange among populations. Haplotype network structure
analyses revealed low levels of shared haplotypes among
populations. Neutrality test and mismatch distribution
results indicated that only the Lijiang population had
experienced obvious population expansion. Overall,
these results indicate that J. sinensis is still a
single evolutionarily significant unit, but when
considering the threat of habitat disturbance to the
population, it is still necessary to carry out
long-term genetic monitoring on J. sinensis and on
other endemic fishes with similar ecological habits in
order to maintain the genetic diversity of fishes in
the upper YR.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bassmann:2023:ESD,
author = "Bj{\"o}rn Ba{\ss}mann and Lisa Hahn and Alexander Rebl
and Lisa Carolina Wenzel and Marc-Christopher
Hildebrand and Marieke Verleih and Harry Wilhelm Palm",
title = "Effects of Stocking Density, Size, and External Stress
on Growth and Welfare of \geoname{African} Catfish
(\bioname{Clarias gariepinus} {Burchell}, 1822) in a
Commercial {RAS}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "74",
day = "26",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020074",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/74",
abstract = "The effects of semi-intensive (100 kg m \minus 3),
intensive (200 kg m \minus 3), and super-intensive (400
kg m \minus 3) stocking densities on the growth and
welfare of African catfish (Clarias gariepinus) were
investigated under commercial production conditions.
Plasma cortisol, glucose, and selected transcripts
following a stress challenge, lactate, as well as skin
lesions, were analyzed at regular intervals (from 12 g
juveniles to 1.5--2.0 kg). The fish grew well, but
after 23 weeks, the semi-intensively stocked fish had a
mean final weight of 1830.5 g, significantly higher
than the super-intensively stocked fish with 1615.4 g,
and considerably higher than the intensively stocked
fish with 1664.8 g (p {$>$} 0.05). Cortisol and glucose
responses significantly differed between stressed and
unstressed fish, but not between treatment groups. An
unforeseen external stressor (nearby demolition noise)
caused stress responses among all treatment groups, but
was similarly coped with. Mortality ranged between
3.8--9.2\%. In the juveniles, skin lesions were reduced
under intensive or super-intensive densities, with the
least under semi-intensive densities in outgrown fish.
Expression profiles of 22 genes were compared in the
spleen at semi-intensive and super-intensive densities.
The transcript concentrations of most genes remained
unchanged, except for slc39a8 and mtf1, which were
significantly downregulated in stressed catfish under
semi-intensive conditions. We demonstrated that African
catfish growth performance and welfare depend on age
and stocking density, also reacting to demolition
noise. This supports farm management to optimize
stocking densities during the grow-out of African
catfish in RAS and suggests avoiding external stress.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tseng:2023:LRP,
author = "Huan-Sheng Tseng and Hsin-Hua Tsai and Po-Hsing
Tseng",
title = "The Labour Rights Protection of Migrant Fishing
Workers in \geoname{Taiwan}: Case Study of
\geoname{Nan-Fang-Ao} Fishing Harbor",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "73",
day = "26",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020073",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/73",
abstract = "Marine fisheries are undeniably important to Taiwan in
terms of economic development and industrial
strategies. In the past 10 years, Taiwan's fishery GDP
accounted for 14\% to 21\% of all agriculture,
forestry, fishery, and animal husbandry, which is
higher than both animal husbandry and forestry. Since
the domestic population structure of Taiwan has
changed, the domestic fishery industrial labor force
has gradually been replaced by migrant fishing workers.
The issue of migrant fishing workers' protection has
received attention from non-governmental organizations,
and cross-national recruitment administration has
become the greatest challenge faced by Taiwan's
authority. Therefore, this paper describes the
necessary protection of the labor conditions and
interests of migrant fishermen by analyzing their
status and human rights protection in the historical
development of Taiwan's marine fisheries. In addition,
considering the well-being of migrant fishing workers,
this paper conducts a feasibility assessment on the
operation of the ``Nan-Fang-Ao Fishermen's Hostel'' at
the Nan-Fang-Ao Fishing Harbor and illustrates the
urgent need to re-examine the migrant fishing workers'
protection. This paper suggests that the government
agencies should actively advise employers to accept
migrant fishermen's requests for better living
environments onshore and guarantee basic living
conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Perri:2023:EMD,
author = "Edward Perri and Leslie Sturmer and Paul S. Wills and
John Baldwin and Susan Laramore",
title = "Effect of Microalgal Diets on Sunray Venus Clam
(\geoname{Macrocallista nimbosa}) Production and Fatty
Acid Profile",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "72",
day = "26",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020072",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/72",
abstract = "The sunray venus (sunray) clam, Macrocallista nimbosa,
is an alternative clam species reared in hard clam
hatcheries in Florida. Current feeding practices follow
those used for hard clam culture. This study aimed to
identify whether a hard clam bi-algal Tisochrysis lutea
and Chaetoceros neogracile diet was an optimal diet for
post-set sunray clams or whether other microalgal
dietary combinations could improve production. Six
dietary bi-, tri-, or tetra-algal combinations
consisting of four microalgae species (Tisochrysis
lutea, Diacronema lutheri, Chaetoceros neogracile, and
Thalassiosira weissflogii) were fed for 6 weeks; the
growth, survival, and fatty acid profiles of post-set
clams were evaluated. Clams fed equal proportions of T.
lutea, D. lutheri, C. neogracile, and T. weissflogii
had higher growth, while those fed equal proportions of
T. lutea and C. neogracile had higher survival. The
poorest-performing diet consisted solely of diatoms. A
contrasting polyunsaturated fatty acid (PUFA) profile
was found in post-set clams fed flagellate- or
diatom-only diets. Clams fed the bi-algal flagellate
diet had a higher percentage of docosahexaenoic acid
(DHA) but a lower percentage of (n-6) PUFA, whereas
those fed the bi-algal diatom diet had a higher
percentage of arachidonic acid (ARA) and
eicosapentaenoic acid (EPA) but a lower percentage of
DHA. The percentages were similar and neither very high
nor very low in clams fed the remaining dietary
treatments. The results of this study show that sunray
venus post-set clams can be successfully produced when
fed a typical hard clam bi-algal flagellate and diatom
diet, but they indicate that growth may be accelerated
by the addition of other microalgae species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pham:2023:TEO,
author = "Ca-Van Pham and Hui-Cheng Wang and Sheng-Hung Chen and
Jie-Min Lee",
title = "The Threshold Effect of Overfishing on Global Fishery
Outputs: International Evidence from a Sustainable
Fishery Perspective",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "71",
day = "24",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020071",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/71",
abstract = "Using global fishery outputs from 62 countries during
the period 2001--2020, this study applies the threshold
panel data model of Hansen (2000) to empirically
explore the nonlinear relations between the number of
fishing vessels, fishers, and fishery production, with
distinct overfishing stocks (overfishing) and fish
caught by trawling or dredging techniques (trawling) as
the threshold variables. Our findings show that the
levels of fishery outputs could be increased by
different combinations of primary factors, such as the
number of fishing vessels and fishers. More
specifically, when the number of fishing vessels and
fishermen operating in countries with a high ratio of
overfishing (overfishing {$>$} 4.4456) increased, their
fishery outputs significantly increased as compared
with countries with a low percentage of overfishing. As
overfishing activities increase, they can result in
significant shortages and imbalances of fishery
resources, directly influencing fish biomass,
biodiversity, and sustainability of fisheries, as well
as making destructive fishing practices worse for
marine ecosystems. Countries with a relatively high
proportion of trawler net usage (trawling {$>$} 4.5516)
would have significantly lower fishery outputs, as more
fishing vessels are invested in them, compared with
countries with a low proportion of trawling net use.
This implies that trawler net usage is a
non-sustainable fishing technique and harms fishery
resources. The policy implications of this study
indicate that taxing overfished species and
implementing trawling regulations could significantly
improve species richness and site-based abundance, thus
helping to sustain fishery outputs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2023:NTA,
author = "Ziyu Zhou and Gu Shan and Xin Li and Wenxuan Guo and
Kexin Ma and Yueyao Yang and Yifan Li and Yunbang Zhang
and He Zhou and Xiaojuan Cao",
title = "De Novo Transcriptome Analysis of the Early Hybrid
Triploid Loach (\bioname{Misgurnus anguillicaudatus})
Provides Novel Insights into Fertility Mechanism",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "70",
day = "23",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020070",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/70",
abstract = "As a new freshwater aquaculture product, triploid
loaches (Misgurnus anguillicaudatus) are characterized
by fast growth, high-quality meat, high edibility, high
resistance to disease, and sterility. In this study, a
natural tetraploid loach (4n = 100) ([female sign]) was
crossed with a diploid loach (2n = 50) ([male sign]),
thus creating the hybrid triploid loach (3n = 75). The
histological observations of triploid offspring and
diploid controls at 4 days post-hatching (dph), 15dph,
22dph, and 50dph showed that most of the hybrid
triploid loaches were abortive in the early gonad
differentiation process. To explore its fertility
mechanism, through transcriptome analyses of triploid
offspring and diploid controls at four periods, 10
differentially expressed genes related to the early
fertility mechanism were identified: amh, hormad1,
rec8, h2b, plvap, zp3, h2a, nrb0b1, ddx4, and esr2.
According to the Kyoto Encyclopedia of Genes and
Genomes (KEGG) enrichment analyses of DEGs, two
pathways were identified that are closely related to
the early fertility mechanism at 50dph: the estrogen
signaling pathway and steroid biosynthesis. The
findings laid a foundation for further exploration of
their molecular inhibition mechanism in hybrid triploid
loaches.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Frid:2023:CES,
author = "Ori Frid and Tal Gavriel and Yigael Ben-Ari and Adi
Weinberger and Hagar Yancovich-Shalom and Jonathan
Belmaker",
title = "Catch Estimates and Species Composition of
Recreational Fishing in \geoname{Israel}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "69",
day = "23",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020069",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/69",
abstract = "Recreational fishing is common around the
Mediterranean Sea. The number of recreational fishers
is growing, and they are using increasingly efficient
fishing techniques. However, fisher activity is
heterogeneous, both temporally and spatially, making it
very difficult to determine this sector's fishing
pressure and annual yields. Therefore, estimates of
annual yields and ecological effects of this fishing
sector are limited. In this study, we undertook an
extensive survey designed to document and quantify
recreational fishing patterns across the Israeli
Mediterranean shoreline. We comprehensively quantified
recreational fishing using three complementary
strategies: (1) ground surveys, including interviews
with anglers on the coast, (2) personalized phone
interviews, and (3) aerial surveys by helicopter. These
methods were used to calculate annual recreational
yield and to estimate species and size composition,
which were then compared to the commercial fishing
catch. We found that a recreational catch makes up
between 10\%-37\% of the total annual fishing yields,
which is similar to estimates from other regions of the
Mediterranean. We also found that non-indigenous
species are among the most common species in
recreational catch and have become a significant part
of local fishery yields. Recreational angling from the
coast targets smaller, reef associated species compared
to recreational fishers at sea. We identified 23
species common to both recreational fishing and
commercial fishing, over which conflicts between
fishing sectors may arise. These results can be used to
more accurately manage Israeli fisheries and can
provide a baseline against which to compare future
changes in a region under the threats of climate
change, biological invasions, and growing human
pressure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sardi:2023:SET,
author = "Adriana E. Sardi and Jos{\'e} M. Moreira and Lisa
Omingo and Xavier Cousin and Marie-Laure B{\'e}gout and
Manuel Manchado and Nina Marn",
title = "Simulating the Effects of Temperature and Food
Availability on True Soles (\bioname{Solea} spp.)
Early-Life History Traits: a Tool for Understanding
Fish Recruitment in Future Climate Change Scenarios",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "68",
day = "22",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020068",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/68",
abstract = "Research on recruitment variability has gained
momentum in the last years, undoubtedly due to the many
unknowns related to climate change impacts. Knowledge
about recruitment --- the process of small, young fish
transitioning to an older, larger life stage --- timing
and success is especially important for commercial fish
species, as it allows predicting the availability of
fish and adapting fishing practices for its sustainable
exploitation. Predicting tools for determining the
combined effect of temperature rise and food quality
and quantity reduction (two expected outcomes of
climate change) on early-life history traits of fish
larvae are valuable for anticipating and adjusting
fishing pressure and policy. Here we use a previously
published and validated dynamic energy budget (DEB)
model for the common sole (Solea solea) and adapt and
use the same DEB model for the Senegalese sole (S.
senegalensis) to predict the effects of temperature and
food availability on Solea spp. early life-history
traits. We create seven simulation scenarios,
recreating RCP 4.5 and 8.5 Intergovernmental Panel on
Climate Change (IPCC) scenarios and including a
reduction in food availability. Our results show that
temperature and food availability both affect the age
at metamorphosis, which is advanced in all scenarios
that include a temperature rise and delayed when food
is limited. Age at puberty was also affected by the
temperature increase but portrayed a more complex
response that is dependent on the spawning (batch)
period. We discuss the implications of our results in a
climate change context.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ji:2023:PTR,
author = "Nanjing Ji and Mingyang Xu and Junyue Wang and Junjia
Li and Shishi Liu and Xueyao Yin and Xin Shen and
Yuefeng Cai",
title = "Physiological and Transcriptomic Response of
\geoname{Asiatic} Hard Clam \bioname{Meretrix meretrix}
to the Harmful Alga \bioname{Heterosigma akashiwo}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "67",
day = "22",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020067",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/67",
abstract = "The ichthyotoxic raphidophyte Heterosigma akashiwo is
associated with harmful algal blooms (HABs). Although
the influence of H. akashiwo on fish has been reported,
little is known of its effects on other marine
organisms, such as shellfish. In this study, the
physiological and transcriptomic responses of the
Asiatic hard clam Meretrix meretrix to H. akashiwo were
investigated. The results showed that clam survival was
not influenced by H. akashiwo (at a concentration of 2
$ \times $ 10 4 cells/mL), and M. meretrix eliminated
the alga effectively after 48 h in co-culture. H.
akashiwo did not alter the antioxidant capacity of the
clams. However, 544 unigenes were found to be
differentially expressed between the control and H.
akashiwo -treated clams. Interestingly, the significant
up-regulation of lysosome-related genes and transport
proteins suggested remodeling of the clam metabolism
possibly for digestion of H. akashiwo. In summary, our
results indicate that the clam M. meretrix can
effectively feed on H. akashiwo, and intensification of
shellfish farming may be helpful to control and
mitigate H. akashiwo blooms.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dezfuli:2023:RCP,
author = "Bahram Sayyaf Dezfuli and Giuseppe Castaldelli and
Massimo Lorenzoni and Antonella Carosi and Mykola
Ovcharenko and Giampaolo Bosi",
title = "Rodlet Cells Provide First Line of Defense against
Swimbladder Nematode and Intestinal Coccidian in
\bioname{Anguilla anguilla}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "66",
day = "21",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020066",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/66",
abstract = "A subpopulation of 97 European eels, Anguilla
anguilla, was obtained from a local consortium of Lake
Trasimeno. The fish were examined for parasites in the
swimbladder (SB) and intestine; the SBs of 66 (68\%) of
the 97 eels contained 480 adult specimens of the
nematode Anguillicoloides crassus (Kuwahara, Niimi and
Itagaki, 1974) and the intensity of infection ranged
from 1 to 18 adult worms per SB (7.27 \pm 0.43, mean
\pm SE). In heavily infected SB, the wall was thicker;
upon excision, black-brownish adult nematodes were
noticed inside the lumen. The infected SBs showed a
papillose aspect of the epithelium and frequent
erosion, inflammation, hemorrhages, and dilation of
blood vessels. In parasitized SBs, mast cells and
macrophages were encountered in the mucosal layer; in
several heavily infected SB, rodlet cells were the sole
fish immune cells noticed in the epithelium and in
close contact with the A. crassus. With reference to
the eel intestinal epithelium, in fifteen eels, two
developmental stages of coccidian were close to
clusters of rodlet cells, the coccidian induced erosion
of the epithelium. The results of a panel of 12
antibodies in the European eel infected tissues will be
presented.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:ICG,
author = "Yan Wang and Zhihong Liu and Xi Chen and Liqing Zhou
and Xiujun Sun and Tao Yu and Xiaomei Wang and Yanxin
Zheng and Biao Wu",
title = "Identification and Characterization of \gene{GYS} and
\gene{GSK3$ \beta $} Provides Insights into the
Regulation of Glycogen Synthesis in \geoname{Jinjiang}
Oyster \bioname{Crassostrea ariakensis}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "65",
day = "21",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020065",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/65",
abstract = "Glycogen, a stored form of glucose, is an important
form of energy for aquatic shellfish, contributing to
the flavor and quality of the oyster. The glycogen
synthase (GYS) and glycogen synthase kinase 3\beta
(GSK3\beta) are two major enzymes in the glycogenesis.
However, the information of the two genes in the
Jinjiang oyster Crassostrea ariakensis remains limited.
In this study, we identified the genes of GYS and
GSK3\beta and further explored their function in the
glycogen synthesis of C. ariakensis. The GYS and
GSK3\beta were distributed in all tested tissues, and
high expression of GYS and glycogen content were
detected in the gonad, labial palp, hepatopancreas, and
mantle, while the high expression of GSK3\beta was
observed in the gill and adductor muscle. The
expression of GYS was positively correlated with the
glycogen content, while GSK3\beta was negatively
correlated. Additionally, knockdown of GSK3\beta using
RNAi decreased the GYS expression, revealing the
negative regulatory effect of GSK3\beta on GYS. These
findings enrich the research data of GSK3\beta and GYS
involved in glycogen synthesis, providing valuable
information for further research on the function of
GSK3\beta and GYS in the glycogen synthesis process of
oyster.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Okeke-Ogbuafor:2023:PSP,
author = "Nwamaka Okeke-Ogbuafor and Tim Gray and Kelechi Ani
and Selina Stead",
title = "Proposed Solutions to the Problems of the
\geoname{Lake Chad} Fisheries: Resilience Lessons for
\geoname{Africa}?",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "64",
day = "20",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020064",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/64",
abstract = "Fishing communities in Lake Chad are experiencing
humanitarian crises --- more than five million people
in the region are hungry and malnourished --- and
fishers are in dire need of improved fisheries
management policies. Understanding the fishers'
resilience, and how they perceive their fisheries
policies, could provide an opportunity for governments
and fisheries managers to refine their policies. The
present study, which is based on 38 semi-structured
interviews carried out between January and April 2022
on the Nigerian shores of Lake Chad, breaks new ground,
firstly by seeking to understand the issues raised by
declining fish stocks in Lake Chad from the viewpoints
of fishers themselves; and secondly by making use of
resilience theory to interpret the fishers' responses
to their situation. Our findings are that the fishers
have a surer grasp of the most effective resilience
strategies available to them than external bodies; and
that the fishers' adaptive resilience and local
knowledge provide a framework for developing smarter
fisheries management policies for Lake Chad. This study
provides evidence to support recommendations for
Africa's supranational, national and local governments
to invest in, and make use of, the fisheries research
on the ground to address the problems facing its
fisheries, rather than experimenting with seemingly
random ideas from across the globe. The Lake Chad
fisheries crisis is an extreme case demonstrating the
harmful effects of external influences from which the
fisheries of other African countries can learn
lessons.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Costa:2023:MMD,
author = "Wilson J. E. M. Costa and Caio R. M. Feltrin and
Jos{\'e} Leonardo O. Mattos and Roger H. Dalcin and
Vinicius Abilhoa and Axel M. Katz",
title = "Morpho-Molecular Discordance? {Re}-Approaching
Systematics of \bioname{Cambeva} ({Siluriformes:
Trichomycteridae}) from the
\geoname{Guaratuba-Babitonga-Itapocu Area, Southern
Brazil}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "63",
day = "20",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020063",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/63",
abstract = "A recent field inventory focusing on catfishes of the
trichomycterine genus Cambeva detected the occurrence
of two morphotypes, C. barbosae and C. cubataonis, in
the Guaratuba-Babitonga-Itapocu area (GBIA) of southern
Brazil, reporting some discordance with results of
coalescent-based approaches for species delimitation
that indicated different estimates of species number.
Contrastingly, based on examination of characters taken
from the external morphology and osteology, we here
recognised six species of Cambeva in GBIA: C. cf.
botuvera, a polymorphic and geographically widespread
species; C. cubataonis, endemic to the Rio Cubat{\~a}o
do Norte; and four new species, two endemic to the Rio
Itapocu basin, one endemic to the Ba{\'\i}a de
Babitonga system and one endemic to the Ba{\'\i}a de
Guaratuba system. We performed a molecular phylogenetic
analysis indicating that Cambeva comprises three major
clades, the alpha-, beta- and gama-clades, with C. cf.
botuvera and a clade comprising C. cubataonis and three
new species belonging to the beta-clade and another new
species belonging to the gama-clade. We concluded that
species here recognised are not in fact incongruent
with results of that recent study when taxa are
correctly identified by a representative sample of
morphological characters, highlighting the importance
of osteological characters for delimiting
trichomycterine species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:NCT,
author = "Sentao Chen and Peng Shi and Qingkai Feng and Xiaoting
Qiu and Jilin Xu and Xiaojun Yan and Chengxu Zhou",
title = "A Novel {C}-Type Lectin and Its Potential Role in
Feeding and Feed Selection in \bioname{Ruditapes
philippinarum}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "62",
day = "19",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020062",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/62",
abstract = "In recent years, the role of lectins in the feed
selection of bivalve has become hot research topic.
Manila clam Ruditapes philippinarum is a species of
marine bivalve with important economic value. A new
C-type lectin (Rpcl) from the clam was obtained and its
potential role in feeding and feed selection was
studied. Rpcl cDNA was 929 bp in length and had 720 bp
of open reading frame. Rpcl encoded 235 amino acids,
comprising a carbohydrate recognition domain (CRD) as
well as an N-terminal signal peptide. Rpcl contained a
conserved CRD disulfide bond including five cysteine
residues (Cys 125, Cys 142, Cys 213, Cys 219, and Cys
237) and the QPN motif (GLN 204 -PRO 205 -ASN 206).
Phylogenetic analysis revealed that the amino acid
sequence of Rpcl was closely related to that of
Vpclec-1 from R. philippinarum and C-type lectin from
Mercenaria. The qPCR analysis indicated that Rpcl
expression was observed in all examined tissues and was
the highest in gills followed by in the hepatopancreas,
and to a lesser extent in the mantle and lip. The in
vitro agglutination experiments showed that, the
purified Rpcl protein could selectively agglutinate
with different microalgae. The strongest agglutinating
effect with Chlorella sp. was observed, followed by
Karlodinium veneficum and Chaetoceros debilis Cleve. No
agglutination with Prorocentrum minimum was observed.
In the feeding experiment, compared with that of the
starvation group, Rpcl expression in the lip and gill
of the clam fed with C. debilis and K. veneficum showed
a significant upward trend with the change of time. In
addition, it was found that the changes in the
expression of the Rpcl gene in the gill and lip, the
main feeding tissues, were consistent with the slope of
the decrease in the number of algal cells in the water
body. In summary, the structure of a new C-type lectin
(Rpcl) was reported in this study and its correlation
with the feeding and feed selection in R. philippinarum
was confirmed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Majhi:2023:ESD,
author = "Snigdha S. Majhi and Soibam Khogen Singh and Pradyut
Biswas and Reshmi Debbarma and Janmejay Parhi and
Soibam Ngasotter and Gusheinzed Waikhom and Dharmendra
Kumar Meena and Ayam Gangarani Devi and Sudhanshu S.
Mahanand and K. A. Martin Xavier and Arun Bhai Patel",
title = "Effect of Stocking Density on Growth, Water Quality
Changes and Cost Efficiency of Butter Catfish
(\bioname{Ompok bimaculatus}) during Seed Rearing in a
Biofloc System",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "61",
day = "19",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020061",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/61",
abstract = "Biofloc technology is a modern-day high-density fish
culture system employing heterotrophic conversion of
nitrogenous waste into useful flocs for water quality
control and improved welfare. However, optimization of
the stocking density for the target species during seed
rearing is the key to a successful farming operation.
The study evaluated the effect of different stocking
density on the growth, feed utilization, digestive
physiology and economics in a biofloc-based seed
rearing of butter catfish, O. bimaculatus. Advanced fry
(1.21 \pm 0.08 g) was reared in a zero-water exchange
biofloc system for 90 days at stocking densities of 0.5
g/L(T1), 1.0 g/L(T2), 1.5 g/L(T3) and 2.0 g/L(T4). The
observed water quality indicates a reduction in DO and
pH in T4, while the total ammonia nitrogen and nitrite
levels remained low in T1. Among the groups, highest
weight gain was noticed in the lowest stocking density
(0.5 g/L) (p {$<$} 0.05), which coincides with a better
feed conversion ratio. Similarly, the digestive enzyme
(protease, amylase, and lipase) secretion was higher in
T1. Profitability assessment describes the possibility
of low profit in T4, in the case where the fish's sale
price is based on harvested size. T1 showed higher
individual growth and higher profit. Overall, a low
stocking density of 0.5 g/Lis optimum for augmenting
growth, feed utilization, physiological function and
economic performance of O. bimaculatus. The study
provides direction for a low-stocking oriented
ecological and economically sustainable method of seed
production of butter catfish in a biofloc system.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Morick:2023:MIP,
author = "Danny Morick and Yuval Maron and Nadav Davidovich and
Ziv Zemah-Shamir and Yaarit Nachum-Biala and Peleg Itay
and Natascha Wosnick and Dan Tchernov and Shimon
Harrus",
title = "Molecular Identification of \bioname{Photobacterium
damselae} in Wild Marine Fish from the \geoname{Eastern
Mediterranean Sea}",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "60",
day = "18",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020060",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/60",
abstract = "Infectious diseases caused by marine bacterial
pathogens inflict increasing economic losses to
fisheries and aquaculture, while also posing a growing
risk to public health and affected species
conservation. In this study, four wild marine fish
species were collected at five fishing sites in Israel,
divided into two regions --- north (Acre, Haifa,
Shefayim) and center-south (Tel-Aviv and Ashdod), and
screened for Photobacterium damselae. An initial
screening was carried out using PCR analysis with
specifically designed primers on DNA extracted from
livers and kidneys. P. damselae -positive samples had
their 16S rRNA amplicons sequenced. Later, an attempt
to specify relevant sub-species was performed, using a
three-layered gene screen: Car, ureC and toxR. Of 334
fish samples, 47 (14\%) were found to be P. damselae
-positive, of which 20 were identified as P. damselae
subsp. piscicida (Phdp), two as P. damselae subsp.
damselae (Phdd) and 25 could not be identified to
subspecies. Our results strengthen the view that fish
residing in a polluted environment are receptive of
pathogenic microorganisms. To assess how the presence
of pathogens may affect population management and
conservation, this research should be followed by
studies aimed at: (i) quantifying levels of pollutants
that may affect pathogen emergence, and (ii) creating a
standard pollution-level index as a basis for setting
criteria, above which authorities should take measures
of precaution.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Baldissera:2023:CKA,
author = "Matheus D. Baldissera and Bernardo Baldisserotto",
title = "Creatine Kinase Activity as an Indicator of Energetic
Impairment and Tissue Damage in Fish: a Review",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "59",
day = "18",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020059",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/59",
abstract = "Creatine kinase (CK) is an enzyme that produces and
uses phosphocreatine to transfer energy to maintain
tissue and cellular energy homeostasis, being
considered the main controller of cellular energy
homeostasis. Its activity in plasma/serum has been
commonly used to evaluate tissue damage, since CK is
released into the bloodstream during damage. This
review summarizes the current knowledge regarding the
use of CK activity in fish, focusing on its potential
as an indicator of the impairment of energetic
homeostasis and tissue damage during stressful
situations, such as exposure to contaminants (metals,
pesticides, microplastic), hypoxia, thermal stress, and
diseases (fungal, parasitic, and bacterial). Based on
the data, we can conclude that tissue CK activity can
be used as a suitable indicator of the impairment of
energetic homeostasis in fish exposed to different
aquaculture challenge conditions, while serum/plasma CK
activity can be used as the first evidence of possible
tissue damage, due to its release into the
bloodstream.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Office:2023:ARF,
author = "Fishes Editorial Office",
title = "Acknowledgment to the Reviewers of
{{\booktitle{Fishes}}} in 2022",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "58",
day = "18",
month = jan,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020058",
ISSN = "2410-3888",
bibdate = "Mon Sep 18 07:38:50 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/58",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Apostolou:2023:FBB,
author = "Apostolos Apostolou and Luchezar Pehlivanov and
Michael Schabuss and Horst Zornig and Georg Wolfram",
title = "Fish Biozonation in the \geoname{Balkan Peninsula},
Especially in \geoname{Bulgaria}: a Challenge",
journal = j-FISHES,
volume = "8",
number = "2",
pages = "91",
day = "03",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8020091",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:31 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/2/91",
abstract = "Fish are important elements of aquatic ecosystems.
Their communities naturally follow the river continuum
and have been well described in the western European
freshwater watersheds. In regions of higher endemism,
such as the Balkan Peninsula, the widely accepted fish
zonation of Illies is doubtfully relevant. In this
study, a more suitable categorization of lotic and
lentic freshwater ecosystems in Bulgaria is proposed,
based on 389 multimethodological and multihabitat fish
sampling occasions. Referent-type communities have been
determined for 33 types of recognized water bodies.
Adapted zonation of Illies is a rough estimation of the
real distribution of fish assemblances in the country;
moreover, sensitive and/or endemic species are
generally of restricted distribution and abundance. In
view of the lentic ecosystems, they can be divided into
salmonid and cyprinid, at approximately 1500 m above
sea level.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:MCE,
author = "Lei Zhang and Peng Shi and Lin Zhang and Yajun Wang
and Jilin Xu and Xiaojun Yan and Kai Liao",
title = "Molecular Characterization and Expression Response of
{Ghrelin}, {GLP-1} and {PYY} to Fasting, Dietary Lipid,
and Fatty Acids in Silver Pomfret (\bioname{Pampus
argenteus})",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "170",
day = "22",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030170",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/170",
abstract = "Ghrelin, glucagon-like peptide-1 (GLP-1), and peptide
YY (PYY) are potent hormones mediating food intake
according to the nutritional status in fish. However,
limited information is available on these genes and
their expression in response to nutrition in silver
pomfret (Pampus argenteus). A comparison analysis
revealed that ghrelin and GLP-1 were relatively
conserved in marine fish. PYYa and PYYb shared a low
identity and were clustered to different PYY branches.
Ghrelin, pyya, and pyyb mRNAs were highly expressed in
the brain, while glp-1 was highly expressed in the
gills and liver. The ghrelin mRNA expression was
relatively high an hour post-feeding and decreased
after 3 to 72 h of fasting. The glp - 1 mRNA expression
was increased after 6 to 24 h of fasting. The pyya mRNA
expression was increased after 72 h of fasting, while
the pyyb expression remained stable during fasting. The
diet with 14\% lipid promoted the expressions of glp-1,
pyya, and pyyb, but inhibited the ghrelin expression.
The ghrelin expression in the intestine was increased
after palmitic acid, oleic acid (OA), linoleic acid
(LA), {\alpha}-linolenic acid, eicosapentaenoic acid
(EPA), and docosahexaenoic acid (DHA) incubation. OA
decreased the expression of glp-1 and increased the
expression of pyya. The expression of pyyb was
downregulated by LA, DHA, and EPA. These results
suggest the importance of ghrelin, glp-1, and pyy in
coordinating food intake in response to fasting,
dietary lipid concentration, and fatty acids in silver
pomfret.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:DFD,
author = "Zhen Wang and Haolu Liu and Guangyue Zhang and Xiao
Yang and Lingmei Wen and Wei Zhao",
title = "Diseased Fish Detection in the Underwater Environment
Using an Improved {YOLOV5} Network for Intensive
Aquaculture",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "169",
day = "21",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030169",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/169",
abstract = "In intensive aquaculture, the real-time detection and
monitoring of common infectious disease is an important
basis for scientific fish epidemic prevention
strategies that can effectively reduce fish mortality
and economic loss. However, low-quality underwater
images and low-identification targets present great
challenges to diseased fish detection. To overcome
these challenges, this paper proposes a diseased fish
detection model, using an improved YOLOV5 network for
aquaculture (DFYOLO). The specific implementation
methods are as follows: (1) the C3 structure is used
instead of the CSPNet structure of the YOLOV5 model to
facilitate the industrial deployment of the algorithm;
(2) all the 3 $ \times $ 3 convolutional kernels in the
backbone network are replaced by a convolutional kernel
group consisting of parallel 3 $ \times $ 3, 1 $ \times
$ 3 and 3 $ \times $ 1 convolutional kernels; and (3)
the convolutional block attention module is added to
the YOLOV5 algorithm. Experimental results in a fishing
ground showed that the DFYOLO is better than that of
the original YOLOV5 network, and the average precision
was improved from 94.52\% to 99.38\% (when the
intersection over union is 0.5), for an increase of
4.86\%. Therefore, the DFYOLO network can effectively
detect diseased fish and is applicable in intensive
aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Contino:2023:URB,
author = "Martina Contino and Greta Ferruggia and Roberta
Pecoraro and Elena Maria Scalisi and Gianfranco
Cavallaro and Carmela Bonaccorso and Cosimo Gianluca
Fortuna and Antonio Salvaggio and Fabiano Capparrucci
and Teresa Bottari and Maria Violetta Brundo",
title = "Uptake Routes and Biodistribution of Polystyrene
Nanoplastics on Zebrafish Larvae and Toxic Effects on
Development",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "168",
day = "20",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030168",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/168",
abstract = "Polystyrene (PS) is the most widely used plastic
polymer. It is mainly used to produce disposable
products. Due to its resistance to degradation, PS can
remain in the environment for a long time. Its
mechanical, physical and biological actions determine
the release of smaller fragments, which are able to
penetrate organisms and accumulate in target organs.
Fertilized Danio rerio eggs were exposed to
concentrations of 10 and 20 mg/L of fluorescent,
amino-modified polystyrene nanoplastics (nPS-NH 2) with
diameters of 100 and 50 nm for 96h, according to OECD
guidelines (2013). Uptake, biodistribution, toxicity,
oxidative stress and apoptosis were evaluated;
moreover, we carried out a simulation to study the
interactions between nPS-NH 2 and defined regions of
three receptors: STRA6, Adgrg6 and CNTN4/APLP2. We
demonstrated that after being internalized, nPS-NH 2
could reach the head and bioaccumulate, especially in
the eyes. Moreover, they could lead to oxidative stress
and apoptosis in the several regions where they
bioaccumulated due to their interaction with receptors.
This study confirmed the danger of nanoplastic wastes
released in the environment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:NCI,
author = "Yanghui Chen and Yuan Li and Dongneng Jiang and Defeng
Zhang and Yu Huang and Jia Cai and Jichang Jian and Bei
Wang",
title = "A New Conditionally Immortalized \geoname{Nile}
Tilapia (\bioname{Oreochromis niloticus}) Heart Cell
Line: Establishment and Functional Characterization as
a Promising Tool for Tilapia Myocarditis Studies",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "167",
day = "18",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030167",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/167",
abstract = "A new cell line named the tilapia heart cell line
(TAH-11) was established from the heart of tilapia
(Oreochromis niloticus) by enzymatic digestion and
mechanical separation. The TAH-11 cell line has been
stably subcultured for over 80 generations and
resuscitated after being frozen in liquid nitrogen for
six months, with exuberant cell growth. The optimal
culture condition of TAH-11 is L-15 medium supplemented
with 10\% fetal bovine serum at 25 {$^\circ $}C.
Sequencing of the mitochondrial 18S rRNAs confirmed
that TAH-11 cells were derived from the tilapia. TAH-11
was also identified as a myocardial cell line based on
the mRNA expression of the troponin I, {\alpha}-actin
and myoglobin genes. Simultaneously, TAH-11 could be
successfully transfected with the GFP reporter gene,
suggesting that the TAH-11 cell line could be used for
exogenous gene expression in vitro. The TAH-11 cells
showed susceptibility to CGSIV, which was demonstrated
by the presence of a severe cytopathic effect,
suggesting that the TAH-11 cell line is an ideal tool
for studying host--virus interaction and potential
vaccines. In addition, the expression of inflammatory
factors in TAH-11 cells can be remarkably induced
following Streptococcus agalactiae or Streptococcus
iniae. The present data lays a foundation to further
explore the mechanism of how Streptococcus spp. causes
tilapia myocarditis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rosati:2023:SEH,
author = "Luigi Rosati and Ivana Caputo and Lill{\`a} Lionetti
and Mayana Karoline Fontes and Camilo Dias Seabra
Pereira and Anna Capaldo",
title = "Side Effects of Human Drug Use: an Overview of the
Consequences of Eels' Exposure to Cocaine",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "166",
day = "17",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030166",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/166",
abstract = "The widespread use of drugs is a global problem which
affects not only humans but also the environment around
them, as research is showing the presence of these
substances in different environmental matrices, like
air, water, and soil. Above all, due to the remarkable
pharmacological properties of drugs, it is discovered
that organisms accidentally exposed to them, as aquatic
organisms, undergo behavioral and physiological changes
that can compromise their health, survival, and
reproduction ability. In addition to this, we must
consider the ability of some drugs to accumulate within
these organisms, thus entering the food chain, and the
possible interactions that drugs in water can establish
with each other and with other possible pollutants,
making the final effects on exposed organisms
unpredictable. This article is an overview of the
effects of one of these drugs, cocaine, one of the
drugs commonly found in the aquatic environment, on
European eel, an endangered species and known
biomonitor of aquatic contamination.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lee:2023:CMGa,
author = "Yong-Suk Lee and Maheshkumar Prakash Patil and Jong-Oh
Kim and Yu-Jin Lee and Yong Bae Seo and Jin-Koo Kim and
Kiran R. Mahale and Gun-Do Kim",
title = "Complete Mitochondrial Genome and Phylogenetic
Position of \bioname{Chirolophis wui} ({Perciformes:
Stichaeidae})",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "165",
day = "16",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030165",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/165",
abstract = "The complete mitochondrial genome of Chirolophis wui
(Wang and Wang, 1935) was sequenced using the Illumina
platform. The genome sequence is 16,522 bp in length
with 54\% A+T content and contains 13 protein coding
genes (PCGs), 22 transfer RNA genes (tRNAs), 2
ribosomal RNA genes (rRNAs), and 1 control region
(D-loop). The H-strand contains 28 genes (12 PCGs, 14
tRNAs, and 2 rRNAs), whereas the L-strand accommodates
9 genes (ND6 and 8 tRNAs). The nucleotide composition
of the mitochondrial genome of C. wui is AT-biased,
accounting for 54.0\%, with an AT skew value of
{-0}.0556 and a GC skew value of {-0}.2043. The
majority of PCGs utilized the start codon, ATG, while
only one gene, COI, utilized the alternative start
codon, GTG. Of the 13 PCGs, 6 genes used the
termination codon (TAA or TGA), whereas 7 genes used
the incomplete termination codon (T or TA). Among the
22 tRNA genes, the tRNA-Leu and tRNA-Ser were found in
duplicates. A phylogenetic tree was constructed using
10 complete mitochondrial genome sequences and
indicated that C. wui has a very close relationship
with C. japonicus and other species in the family
Stichaeidae, with a high supporting bootstrap value.
This study can provide valuable information for future
evolutionary studies on C. wui and Stichaeidae.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Adhikari:2023:PEV,
author = "Dhurba Adhikari and Ida K. Hanssen and Steinar D.
Johansen and Truls B. Moum and Jarle T. Nordeide",
title = "{Pitx 1} Enhancer Variants in Spined and Spine-Reduced
\geoname{Subarctic European} Sticklebacks",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "164",
day = "15",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030164",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/164",
abstract = "Loss of body armour, sometimes including a reduction
in or loss of pelvic spines, is an adaptation observed
in many isolated freshwater populations. Pelvic
reduction in sticklebacks has previously been
associated with recurrent, but variant, deletions
within pelvic enhancer regions Pel A and Pel B, which
regulate expression of the homeodomain transcription
factor gene Pitx 1. We investigated variation in
nucleotide sequences of pelvic enhancers in
sticklebacks collected from two small freshwater lakes
in the same watercourse and a nearby marine site in
subarctic Norway. Spineless, as well as asymmetrically
spined and completely spined sticklebacks are present
in the upper lake, while only specimens with complete
spines are found at the other lake and the marine site.
Observed variation at Pel A between the three sites was
mainly due to variable numbers of repeats at three
fragile TG-repeat loci. The length of Pel A, mainly at
one of the TG-repeat loci, was consistently shorter
among individuals in the upper lake compared with
specimens from the two other sites. However, no obvious
association was revealed between enhancer variants and
pelvic status. No polymorphism was found at Pel B.
Thus, additional genetic factors and/or environmental
cues need to be identified to fully explain the
occurrence of pelvic reduction in sticklebacks in this
lake.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Carvalho:2023:ITS,
author = "Andrezza Carvalho and L{\'e}a Carolina de Oliveira
Costa and Mariana Holanda and Lu{\'\i}s H. Poersch and
Gamze Turan",
title = "Influence of Total Suspended Solids on the Growth of
the Sea Lettuce \bioname{Ulva lactuca} Integrated with
the \geoname{Pacific} White Shrimp \bioname{Litopenaeus
vannamei} in a Biofloc System",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "163",
day = "15",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030163",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/163",
abstract = "A biofloc system is rich in nutrients, which favors
the cultivation of macroalgae, but the influence of the
system on the performance of macroalgae is unknown. The
objective of this study was to analyze the feasibility
of introducing the macroalgae Ulva lactuca into the
culture of Litopenaeus vannamei in a biofloc system.
The first experiment evaluated the influence of 400 mg
L {-1} and 30 mg L {-1} solids concentration of the
system in biofloc and von Stosch culture medium on
macroalgae growth. In the second experiment, the
densities of 1, 2, and 3 g L {-1} of U. lactuca were
cultivated in an integrated system with shrimp and
monoculture treatment. Both experiments had 35 days of
cultivation. There was no significant difference in
macroalgae growth between the treatments with biofloc
and von Stosch culture medium. In the integrated
culture, the density of 1 g L {-1} showed better
nutrient absorption. Shrimp performance was not
affected by macroalgae cultivation. In conclusion, the
solids did not affect the growth of the macroalgae, and
it could be cultivated in a biofloc system for nitrate
uptake in integrated culture with shrimp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fuentes:2023:PGC,
author = "Sandra Ferrada Fuentes and Ricardo Galleguillos and
Victoria Herrera-Y{\'a}{\~n}ez and Cristian B.
Canales-Aguirre",
title = "Population Genetics of \geoname{Chilean} Jack
Mackerel, \bioname{Trachurus murphyi} {Nichols}, 1920,
({Pisces, Carangidae}), in Waters of the \geoname{South
Pacific Ocean}",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "162",
day = "13",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030162",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/162",
abstract = "Estimating genetic diversity and population structure
with polymorphic markers might provide information for
the better management of fish that have economic
importance. We used the Chilean jack mackerel,
Trachurus murphyi, a pelagic species distributed in the
south Pacific Ocean, to test the population genetic
structure across its distribution. A total of 522
samples were collected from 11 locations in 2008, to be
analyzed using six loci microsatellites. We conducted
traditional genetic analyses to describe the genetic
diversity of T. murphyi and whether it shows spatial
genetic structure. The results showed a high genetic
diversity across locations (Ho = 0.551 to 0.980; He =
0.676 to 0.959). Estimates for the population structure
showed a low and non-significant pairwise F ST in all
comparisons. We supported the non-genetic
differentiation previously reported with the used
microsatellite panel. Further comparative temporal
studies should be conducted to identify the stability
of this pattern. Overall, this study reinforces the
hypothesis that, in the Pacific Ocean, Chilean jack
mackerel correspond to a large single population.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2023:CSA,
author = "Hansoo Kim and Sungho Cho and Jee Woong Choi and
Donhyug Kang",
title = "Characteristics of Sound Attenuation by Individual and
Multiple Fishes",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "161",
day = "13",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030161",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/161",
abstract = "Fish biomass and stock assessment are estimated from
acoustic volume backscattering strengths (Sv) obtained
from various hydroacoustic equipment. Although sound
attenuation due to fish schools and water influences
the Sv value, only attenuation from water is considered
during the acoustic data process. For these reasons, it
is necessary to understand the characteristics of sound
attenuation by fish. Unfortunately, little is known
about sound attenuation from fish. In the present
study, the attenuation from one to four fish specimens
was precisely measured during ex situ experiments in a
water tank. The scientific echo sounder of a split-beam
200 kHz transducer and a miniature hydrophone were used
for the attenuation measurements. Results show that the
maximum attenuation coefficient ({\alpha}) was
approximately 25 dB/cm when the 4 multiple fishes had
high fish heights. The relationship between the
attenuation coefficient ({\alpha}200kHz) and the total
fish height (Htotal) was approximately
{\alpha}200kHz=0.54{\ast}Htotal {\pm}$ \approx $0.06
(r2=0.72). This work describes the sound attenuation
characteristics to provide basic information for the
compensation of Sv from fish schools or layers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Santander-Neto:2023:AGT,
author = "Jones Santander-Neto and Francisco Marcante Santana
and Jonas Eloi Vasconcelos-Filho and Ros{\^a}ngela
Lessa",
title = "Age and Growth of the Threatened Smalleye Round Ray,
\bioname{Urotrygon microphthalmum}, {Delsman}, 1941,
from \geoname{Northeastern Brazil}",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "160",
day = "12",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030160",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/160",
abstract = "The age and growth of Urotrygon microphthalmum were
studied using specimens captured between March 2010 and
March 2012 as by-catch in the shrimp trawl fishery off
the coast of the state of Pernambuco, Brazil. A total
of 347 vertebrae were read, 161 from males
(81.6--249.55 mm) and 186 from females (86.15--298.1
mm). The estimated average percentage index (IAPE)
ranged from 0.71\% to 4.33\% (mean = 2.5\%) in
vertebrae from specimens with 1 and 6 band pairs,
respectively. In the present study, the different
approaches to validation produced variable results
(partially valid growth zones). We then decided to
discuss the growth of the species considering the
formation of an annual ring. There were statistically
significant differences in growth between the sexes.
The best model to describe male growth was the von
Bertalanffy growth model for two phases (VBGM TP) with
growth parameters L {\infty} (maximum theoretical
length) = 230.35 mm, k (growth constant) = 1.00, t 0
(theoretical age of size zero) = {-0}.76 years and for
females it was the von Bertalanffy with birth size
(VBGM L 0) model with parameters L {\infty} = 282.55
mm, k = 0.37. The age of maturity for males and females
was 1.52 and 2.02 years, respectively, and the maximum
age observed was 5.5 and 8.5 years, respectively.
Despite being a fast-growing species, Urotrygon
microphthalmum is threatened, probably due to the high
mortality levels from shrimp trawling in a very narrow
range of the shelf where all the life stages are
captured.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Smith:2023:ALL,
author = "Cheyenne R. Smith and Christopher A. Ottinger and
Heather L. Walsh and Patricia M. Mazik and Vicki S.
Blazer",
title = "Application of a Lipopolysaccharide ({LPS})-Stimulated
Mitogenesis Assay in Smallmouth Bass
(\bioname{Micropterus dolomieu}) to Augment Wild Fish
Health Studies",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "159",
day = "10",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030159",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/159",
abstract = "The utility of a functional immune assay for
smallmouth bass (Micropterus dolomieu) lymphocyte
mitogenesis was evaluated. Wild populations in the
Potomac River have faced disease and mortality with
immunosuppression from exposure to chemical
contaminants a suspected component. However, a
validated set of immune parameters to screen for
immunosuppression in wild fish populations is not
available. Prior to use in ecotoxicology studies,
ancillary factors influencing the mitogenic response
need to be understood. The assay was field-tested with
fish collected from three sites in West Virginia as
part of health assessments occurring in spring
(pre-spawn; April--May) and fall (recrudescence;
October--November). Anterior kidney leukocytes were
exposed to lipopolysaccharide (LPS) from E.coli O111:B4
or mitogen-free media and proliferation was measured
using imaging flow cytometry with advanced machine
learning to distinguish lymphocytes. An anti-smallmouth
bass IgM monoclonal antibody was used to identify IgM+
lymphocytes. Lymphocyte mitogenesis, or proliferative
responses, varied by site and season and positively and
negatively correlated with factors such as sex, age,
tissue parasites, and macrophage aggregates. Background
proliferation of {IgM-} lymphocytes was negatively
correlated to LPS-induced proliferation in both seasons
at all sites, but only in spring for IgM+ lymphocytes.
The results demonstrate that many factors, in addition
to chemical contaminants, may influence lymphocyte
proliferation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nambi:2023:ILC,
author = "Rebecca Walugembe Nambi and Abebe Getahun and Fredrick
Jones Muyodi and Edward Rukuunya",
title = "Impacts of Land Cover Changes on Catches of
\geoname{Nile} Perch and \geoname{Nile} Tilapia on
\geoname{Lake Kyoga, Uganda}",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "158",
day = "10",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030158",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/158",
abstract = "In East Africa, Nile perch and Nile tilapia are major
commercial fish species, providing reliable protein and
income sources. However, their stocks are dwindling on
Lake Kyoga due to land use and land cover (LULC)
changes within the surrounding catchment. Thus, this
paper aims at assessing the land use land cover on Lake
Kyoga and its immediate catchment and how those changes
affect the water quality and the local fish catches.
The Iso-Cluster tool in ArcGIS was used to analyze
Landsat images from the years 1989 to 2021 to obtain
LULC information. The LULC classes were classified into
water, wetland, agricultural land, rangeland, and
settlements. Correction using a 2-tailed test of the
water quality parameters with the LULC classes
indicated a strong positive correlation of TN with
water (0.71), a strong negative correlation of TP with
rangelands ({-0}.83), and a strong positive correlation
of Chl- a with settlement (0.98) at a 0.05 confidence
level. The correlation of the recorded fish catch data
with fishing gear showed a strong negative correlation
with cast and monofilament nets. Continued use of
illegal fishing gear was ranked number 1 of the causes
of LULC by the stakeholders. Thus, the fisheries
managers should collaborate with local stakeholders to
boost declining fish catches for improved livelihoods
and sustainable management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{daSilveira:2023:IAM,
author = "Estevan Luiz da Silveira and Nabil Semmar and Eduardo
Luis Cupertino Ballester and Andr{\'e} Martins
Vaz-dos-Santos",
title = "Integrative Analysis to Manage Aquatic Resources Based
on Fish Feeding Patterns in Neotropical Rivers",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "157",
day = "06",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030157",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/157",
abstract = "Feeding ecology is an integrative procedure to
highlight different diets, associating feeding trends
with governing and regulation factors characterizing
foraging species and their environments, respectively.
The diet variability of seven fish species forming a
community in a Neotropical riverine system was analyzed
to characterize the resource and consumer linkages,
providing a detailed assessment of adaptive feeding
behaviors of fishes living in different ecological
states transiting from natural/resilient spaces to
anthropic pressions-linked disturbed ones. Fishes were
sampled along four sites during a year, and their
stomach contents were analyzed. Feeding data were
analyzed by applying quali- and quantitative methods
with multi-levels and multifactor aspects to determine
target food categories (percentage of occurrence) and
identify feeding patterns (correspondence and cluster
analyses, CA-HCA). Factors and scales governing target
food categories were also tested. A total of 483
stomachs were dissected, and 30 food categories were
identified. CA-HCA highlighted 10 feeding trends (FTs)
combining several foods co-occurring at distinct
levels. These FTs indicated characteristic diets and
high diversity of feeding behaviors concerning multiple
and narrow diets, different alimentary needs related to
ecomorphological features, different plasticity ranges
(adaptability, tolerance) and a spatial governing
effect (headwater to river mouth environmental quality
loss). These multiple factors provided essential
information on overcoming ways of environmental
constraints and optimization ways of food balances
helping to better manage the richness and working of
neotropical river systems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Velazquez-Landa:2023:ZSB,
author = "Xiomara Vel{\'a}zquez-Landa and Porfirio Carrillo and
Genaro A. Coria-Avila and Deissy Herrera-Covarrubias
and Luis I. Garc{\'\i}a and Mar{\'\i}a Rebeca
Toledo-C{\'a}rdenas and Mar{\'\i}a Elena
Hern{\'a}ndez-Aguilar and Jorge Manzo",
title = "Zebrafish Sexual Behavior in Plain and Enriched
Environments: Parameters in the Valproate Model of
Autism",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "156",
day = "05",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030156",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/156",
abstract = "Autism is a complex alteration in children's
developing nervous system that manifests in behavioral
patterns that do not match those of typical subjects.
Moreover, starting at puberty, these children may
encounter problems regarding social interaction related
to sexual encounters. As studies are scarce, we used
the valproate model of autism in the zebrafish to
contribute to the knowledge related to sexual behavior
in this disorder. Young zebrafish were reproduced,
embryos collected, and organized in groups of control
or treated with valproic acid, as the autism model.
Sexual behavior was recorded in fish from these embryos
as they became sexually active. The results show that
the zebrafish's sexual behavior is organized into
appetitive, preconsummatory, and consummatory
behaviors, as in other vertebrates. In the autism
model, the patterns of sexual behavior are present but
with significant modifications. The behavior of males
was the most affected, while in females, the most
affected parameter was oviposition. These results show
that, in the autism model of zebrafish, sexual behavior
is not suppressed, but it seems that critical changes
occur in the neuroendocrine system that reduces
reproductive success. Furthermore, the enriched
environment was beneficial in maintaining the
consummatory behaviors of females and males with
autism-like behaviors.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Curiel-Bernal:2023:MEG,
author = "Marcelo V. Curiel-Bernal and Miguel {\'A}.
Cisneros-Mata and Guillermo
Rodr{\'\i}guez-Dom{\'\i}nguez and Laura
S{\'a}nchez-Velasco and S. Patricia A.
Jim{\'e}nez-Rosenberg and Alejandro Par{\'e}s-Sierra
and E. Alberto Arag{\'o}n-Noriega",
title = "Modelling Early Growth of \bioname{Totoaba macdonaldi}
({Teleostei: Sciaenidae}) under Laboratory Conditions",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "155",
day = "04",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030155",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/155",
abstract = "The totoaba (Totoaba macdonaldi) is a sciaenid
(croaker) fish endemic to the Gulf of California with
high commercial importance. Because it was considered
at risk of extinction (since 2021 it was reclassified
as vulnerable by the IUCN), and aquaculture procedures
were developed for restocking and commercial purposes.
The present study was conducted with the hypothesis
that the early stages of totoaba present depensatory
individual growth and an observed variance-at-age
modelling approach is the best way to parametrize
growth. Ten models were tested including asymptotic,
non-asymptotic, exponential-like, and power-like curves
including a new one that represents a modification of
Schnute's model. The model that best described the
growth trajectory of larval and early juveniles of T.
macdonaldi in a controlled environment is a sigmoid
curve with two inflexions, related to changes in the
feeding regime.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jayasinghe:2023:DMC,
author = "G. D. T. M. Jayasinghe and Joanna Szpunar and Ryszard
Lobinski and E. M. R. K. B. Edirisinghe",
title = "Determination of Multi-Class Antibiotics Residues in
Farmed Fish and Shrimp from \geoname{Sri Lanka} by
Ultra Performance Liquid Chromatography-Tandem Mass
Spectrometry ({UPLC-MS\slash MS})",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "154",
day = "04",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030154",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/154",
abstract = "Antibiotics have been used to control the aquatic
environment in both therapeutic and prophylactic ways.
Antibiotics are particularly difficult to extract due
to their strong interactions with biological matrices.
In this study, UPLC-MS/MS method was developed and
validated for quantitative confirmatory analysis of
multi-class antibiotics residues in fish and shrimp.
Fourteen antibiotics belonging to sulphonamides,
{\beta}-lactams, quinolones, sulfones and macrolides
were determined within one chromatographic run. The
samples were suspended in 0.1 M HCl, and the analytes
were extracted into ethyl acetate. The extracts were
defatted with cyclohexane. The limits of quantification
(LOQ) ranged from 0.24 to 1.32 {\SGMLmicro}g kg {-1}
for fish and 0.42--1.62 {\SGMLmicro}g kg {-1} for
shrimp samples. The recoveries ranged from 75 to 105\%.
The method was applied to the analysis of farmed
freshwater Tilapia fish (Oreochromis niloticus) and
shrimp (Penaeus monodon) collected in Sri Lanka.
Sulfacetamide (4.31 {\pm} 0.70 {\SGMLmicro}g kg {-1})
and sulfamethoxypyridazine (0.75 {\pm} 0.15
{\SGMLmicro}g kg {-1}) were detected in the fish, and
sulfapyridine (0.21--0.56 {\SGMLmicro}g kg {-1}) and
sulfadoxine (0.35--1.44 {\SGMLmicro}g kg {-1}) were
detected in the shrimp samples. The concentrations
complied with the EU regulation limits for veterinary
drug residues in seafood and did not pose a risk in
terms of food safety.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nastac:2023:PEK,
author = "Lacr{\u{a}}mioara (Gr{\u{a}}dinariu) N{\u{a}}stac and
Lorena Dediu and Mirela Cre{\c{t}}u and Cristian
R{\^\i}mniceanu and Angelica Docan and Iulia Grecu and
Floricel Maricel Dima and Maria Desimira Stroe and
Camelia Vizireanu",
title = "The Protective Effects of Korill Product on Carp
Fingerlings Reared in High Densities and Challenged
with Albendazole Treatment",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "153",
day = "04",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030153",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/153",
abstract = "The objectives of the present study were to evaluate
the potential of the Korill (KO), a product based on
krill oil, supplemented in fish feed (5 g/kg feed) to
alleviate density-induced stress in carp fingerlings,
and its protective capacity in case of anthelmintic
drug administration (ABZ---albendazole). Thus, the fish
were divided into four groups: LD (low density with
normal feed), LD-KO (low density with KO
supplementation feed), HD (high density with normal
feed), and HD-KO (high density with KO supplementation
feed). During the first trial, the fish held under
different densities were fed normal feed and KO feed
for two months, following a 2 $ \times $ 2 factorial
experimental design. In the second trial, seven fish
per tank were subjected, for one week, to albendazole
treatment (administered daily by an oral dose of 5
mg/kg body weight). For both trials, blood and plasma
samples were used to quantify hematological and
biochemical parameters. The results showed that the KO
diet alleviated the negative impact of ABZ treatment on
liver function and the metabolic profile of carp
fingerlings reared in high densities. In addition, KO
feeding improved lysozyme activity (LZM) and therefore
the immune status of the fish, and reduced oxidative
damage in the liver, demonstrated by a decrease of
malondialdehyde (MDA) content and an increase of total
antioxidant capacity (TAC).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Leng:2023:SUA,
author = "Xiaoqian Leng and Hao Du and Wei Xiong and Peilin
Cheng and Jiang Luo and Jinming Wu",
title = "Successful Ultrasonography-Assisted Artificial
Reproduction of Critically Endangered \geoname{Sichuan}
taimen (\bioname{Hucho bleekeri})",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "152",
day = "03",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030152",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/152",
abstract = "Sichuan taimen (Hucho bleekeri), a critically
endangered species, is listed as a first-class
protected species in China. The natural population has
been missing from most of its known distribution areas
for many years but was rediscovered in the Taibai River
in 2012, providing an important resource for the
conservation stocking of the Sichuan taimen. In this
study, 43 wild adult Sichuan taimen collected from the
Taibai River were determined for gamete maturity for
artificial reproduction using ultrasonography. The
echogenicity of the gonads before and after hormonal
induction was described to determine broodstock
identification, injection dose of hormones, and
spawning time. Four batches of artificial breeding were
successfully achieved in 2013, 2014, and 2016.
Broodstock (19 females and 16 males) spawned with
average fertilization and hatching rates reaching
80.2\% and 61.0\%, respectively. This study
demonstrates that ultrasonic imaging is a convenient
and efficient method to track the state of gonads of
mature Sichuan taimen during artificial reproduction,
which will assist in broodstock cultivation, and
further large-scale proliferation and effective
resource restoration of the Sichuan taimen.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Engle:2023:RCB,
author = "Carole R. Engle and Jonathan van Senten and Charles
Clark and Noah Boldt",
title = "Has the Regulatory Compliance Burden Reduced
Competitiveness of the \geoname{U.S.} Tilapia
Industry?",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "151",
day = "02",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030151",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/151",
abstract = "Emerging research on aquaculture governance has
pointed to the conundrum of negative global
environmental effects from economic incentives for
aquaculture production to shift from more highly
regulated to less regulated countries. This study has
focused on examining whether regulatory costs on U.S.
tilapia farms may have contributed to their contraction
in contrast to the growth of global tilapia production
that contributes to the volume of seafood imports into
the U.S. A national survey (coverage rate = 75\% of
tilapia sold; response rate = 18\%) found that on-farm
regulatory costs accounted for 15\% of total production
costs on U.S. tilapia farms, the fifth-highest cost of
production. The total direct regulatory costs
nationally were \$4.4 million, averaging
\$137,611/farm. Most problematic were regulations of
effluent discharge, predatory bird control,
international export, and water and energy policies.
Manpower costs for monitoring and reporting were the
greatest cost of regulatory compliance. The lost sales
revenue resulting from regulations was \$32 million a
year, or 82\% of total annual sales, indicating that
the regulatory framework has constrained the growth of
U.S. tilapia farming. The smallest tilapia farms had
the greatest regulatory cost per kg. This study
provides evidence that regulatory costs, along with
other challenges related to live fish markets, have
contributed to the decline in U.S. tilapia production.
Increased competitiveness of the U.S. tilapia industry
will require a combination of: (1) improved regulatory
efficiency that reduces on-farm cost burdens without
reducing societal benefits; (2) research and on-farm
extension assistance to evaluate new tilapia fillet
equipment; and (3) research on changing consumer
preferences to provide guidance on effective strategies
to penetrate the large U.S. fillet market.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lee:2023:CMGb,
author = "Yong-Suk Lee and Maheshkumar Prakash Patil and Jong-Oh
Kim and Yu-Jin Lee and Yong Bae Seo and Jin-Koo Kim and
Rahul K. Suryawanshi and Gun-Do Kim",
title = "The Complete Mitochondrial Genome of the Fivespot
Flounder, \bioname{Pseudorhombus pentophthalmus}
({Pleuronectiformes: Paralichthyidae}), from
\geoname{Korea} and Its Phylogenetic Analysis",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "150",
day = "02",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030150",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/150",
abstract = "The mitogenome is an important tool for researching
the evolution of metazoan animals. However, until now,
only few mitochondrial genes of Pseudorhombus
pentophthalmus have been reported. Here, we report the
complete mitogenome of P. pentophthalmus, assembled
using the Illumina platform. The circular mitogenome of
P. pentophthalmus is 16,684 bp in length, has a bias
A+T content of 52.78\%, encodes 37 genes (13
protein-coding genes, 22 tRNA genes, 2 rRNA genes), and
has a control region. The overall nucleotide
composition was A: 26.56\%, T: 26.22\%, G: 17.97\%, and
C: 29.25\%. The phylogenetic tree based on the complete
mitogenome P. pentophthalmus was shown to be
monophyletic with the other Pseudorhombus species and
was shown to be on the same branch as P.
dupliciocellatus. This research might be useful for
future studies on population genetics and evolution
analysis.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Becker:2023:EHB,
author = "Alessandra Jana{\'\i}na Becker and Sandro Santos and
Berta Maria Heinzmann and Braulio Otomar Caron and
Bernardo Baldisserotto",
title = "Exposure of \bioname{Hyalella bonariensis}
({Crustacea, Amphipoda}) to Essential Oils: Effects on
Anesthesia and Swimming Activity",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "149",
day = "02",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030149",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/149",
abstract = "Amphipods are frequently used as bioindicators of
water quality in experimental or behavior trials. Thus,
it is a group considered suitable for use as a model
organism in tests with essential oils (EOs). This study
evaluated the time required for anesthesia induction
and recovery of the amphipod crustacean H. bonariensis
exposed to the essential oils of Aloysia triphylla
(EOAT) and Lippia alba (EOLA), and their major
compounds citral and linalool, respectively. In
addition, we evaluated the locomotor activity of
amphipods using ANY-maze {\reg} software. Mortalities
were observed at concentrations of 100 and 200
{\SGMLmicro}L/L of citral (50.0 {\pm} 0.39\%) and 750
{\SGMLmicro}L/L of EOLA (66.7 {\pm} 0.33\%). Except for
linalool, increased concentrations of the compounds of
the essential oils decreased the time for sedation and
anesthesia induction. There were differences for the
induction of anesthesia (p {$<$} 0.05) and recovery (p
{$<$} 0.05) between EOLA and linalool treatments, but
not between that for EOAT and citral. Reduced locomotor
activity and longer time and episodes of freezing were
observed in animals exposed to EOAT. The EOs and their
major compounds induced anesthesia and affected the
locomotor activity of H. bonariensis, Therefore, EOAT
and linalool are recommended for anesthesia of this
species. EOAT can also be utilized in long-term
exposure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shakweer:2023:CIR,
author = "Medhat S. Shakweer and Gehad E. Elshopakey and
Abdelwahab A. Abdelwarith and Elsayed M. Younis and
Simon John Davies and Samia Elbahnaswy",
title = "Comparison of Immune Response of \bioname{Litopenaeus
vannamei} Shrimp Naturally Infected with
\bioname{Vibrio} Species, and after Being Fed with
Florfenicol",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "148",
day = "02",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030148",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/148",
abstract = "The outbreaks caused by Vibrio spp. are a notable
threat to the potential growth of the economy of
penaeid culture, which is still controlled by the
administration of antibiotics. At first, the infected
group was subjected to phenotypic bacteriological
examination with subsequent molecular identification
via 16S rRNA gene sequencing, which confirmed four
strains of Vibrio spp., V. atlanticus, V. natriegens,
V. alginolyticus, and V. harveyi, from
moribund-infected shrimp during mortality events in an
Egyptian hatchery. To better understand the defense
mechanism of the most effective antibiotic against
Vibrio strains, the immune responses were compared and
evaluated in infected Litopenaeus vannamei broodstock
after being fed 5 mg kg {-1} of florfenicol antibiotic,
which was first determined through in vitro antibiogram
tests. Therefore, our study aimed to determine the
immune response of L. vannamei during Vibrio spp.
infection in Egyptian hatcheries and after antibiotic
medication. The parameters assessed were the total and
differential hemocyte count (THC), granular cells (GC),
semi-granular cells (SGC), and hyaline cells (HC). As
well as the metabolic and immune enzymes: alanine
aminotransferases (ALT), aspartate aminotransferases
(AST), alkaline phosphatase (ALP), acid phosphatase
(ACP), and lysozyme activity; an antioxidant index,
such as superoxide dismutase (SOD) and glutathione
(GSH); a phagocytic assay; changes in reactive oxygen
species (ROS); and bactericidal activity in the
hemolymph of the control, infected, and treated groups.
Further evaluation of the mRNA expression levels of the
prophenoloxidase (LvproPO), toll-like receptor 1
(LvToll1), and haemocyanin (LvHc) genes were performed
in the hepatopancreas of the same groups. A significant
drop in the THC, GC, SGC, and HC counts, as well as
lysozyme and bactericidal activities, phagocytic assay,
ROS, SOD, and GSH index, were represented in infected
shrimp compared to control shrimp; however, a marked
increase in the activity of ALT, AST, ALP, and ACP was
observed. These activities were significantly restored
in the treated shrimp compared to the infected shrimp.
Nevertheless, no significant changes were noted in the
transcriptional levels of the LvproPO and LvToll1 genes
in the treated shrimp when compared to the infected
shrimp; however, a significant suppression of the LvHc
gene was noted. Our study aimed to determine the immune
response of L. vannamei during Vibrio spp. infection in
Egyptian hatcheries and after antibiotic medication. We
concluded that florfenicol in medicated feed could be
effective in controlling vibriosis and ameliorating the
immune response of shrimp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Suma:2023:BEG,
author = "Afrina Yeasmin Suma and Shishir Kumar Nandi and
Zulhisyam Abdul Kari and Khang Wen Goh and Lee Seong
Wei and Albaris B. Tahiluddin and Paul Seguin and
Mikael Herault and Abdullah {Al Mamun} and Guillermo
T{\'e}llez-Isa{\'\i}as and Muhammad Anamul Kabir",
title = "Beneficial Effects of Graded Levels of Fish Protein
Hydrolysate ({FPH}) on the Growth Performance, Blood
Biochemistry, Liver and Intestinal Health, Economics
Efficiency, and Disease Resistance to
\bioname{Aeromonas hydrophila} of {Pabda}
(\bioname{Ompok pabda}) Fingerling",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "147",
day = "02",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030147",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/147",
abstract = "Fish protein hydrolysate (FPH) is the enzymatic
hydrolysis of protein into smaller peptide and free
amino acids, which has recently captured considerable
attention as a supplementary ingredient in the
aqua-feed industry sector. The present research aimed
to observe the physiological, biochemical, and
bacteriological study of FPH-treated diets and its
effects on growth, hematology, plasma biochemistry,
liver and gut histopathology, and resistance to
Aeromonas hydrophila infection in Pabda (Ompok pabda).
Four experimental diets (35\% crude protein) were
formulated with graded FPH supplementation (0, 0.5, 1,
and 2\%). The feed physiological parameters such as
expansion ratio, bulk density, water stability, and
floatability were not significantly affected by dietary
FPH levels (p {$>$} 0.05), except for the pellet
durability index (PDI). Furthermore, the diets with 1\%
and 2\% FPH were more palatable to fish than other
treatment diets. The total bacteria (TB) in fish diets
and guts followed an increasing trend with the increase
in various levels of FPH in diets. The significantly
highest body weight, specific growth rate (SGR), total
biomass (TB), survival rate (SR), condition factor
(CF), and hepatosomatic index (HSI) were noted in 2\%
FPH-fed fish when compared with other treatment groups
(p {$<$} 0.05). The feed intake of fish was
significantly increased when increasing the FPH in
diets (p {$<$} 0.05). The fish fed with a 2\% FPH diet
had significantly higher neutrophil, monocyte,
lymphocyte, red blood cell, and platelet levels (p
{$<$} 0.05). The blood glucose, creatinine, total
protein, and globulin were significantly lower in
control fish compared to other treatment groups (p
{$<$} 0.05). The histopathological observation of mid
intestine tissues displayed that 2\% of FPH-diet-fed
fish had a well-anchored epithelial wall with
well-arranged goblet cells, a long villus structure,
stratum compactum, and tunica muscularis compared to
other treatments of FPH. The inclusion of FPH in diets
up to 2\% significantly improved the liver health of
fish. The fish fed with 2\% FPH had a significantly
lower cumulative percent mortality (16.67\%) against A.
hydrophila infection in the bacterial challenge test (p
{$<$} 0.05). Therefore, the present results suggested
that using 2\% FPH in the aqua-feed industry improves
the growth performance, health status, and disease
resistance of Pabda fingerlings in captivity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Paolucci:2023:FNF,
author = "Marina Paolucci",
title = "Fish Nutrition and Feed Technology",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "146",
day = "28",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030146",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/146",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ren:2023:CTA,
author = "Yuanhao Ren and Wei Wang and Yin Fu and Zhiqiang Liu
and Ming Zhao and Likun Xu and Tianyong Zhan and Ting
Huang and Minghao Luo and Wei Chen and Chunyan Ma and
Fengying Zhang and Keji Jiang and Lingbo Ma",
title = "Comparative Transcriptome Analysis Identifies {MAPK}
Signaling Pathway Associated with Regulating Ovarian
Lipid Metabolism during Vitellogenesis in the Mud Crab,
\bioname{Scylla paramamosain}",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "145",
day = "28",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030145",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/145",
abstract = "The mud crab, Scylla paramamosain, has abundant
nutrients in the ovary, where numerous lipids
accumulate during ovarian maturation. However, the
mechanism behind the accumulation of lipids in the
ovary of mud crab during ovarian maturation is largely
unknown. This study conducted a comparative
transcriptome analysis of the ovaries of mud crabs at
various stages of ovarian maturation. A total of 63.69
Gb of clean data was obtained, with a Q30 of 93.34\%,
and 81,893 unigenes were identified, including 10,996
differentially expressed genes (DEGs). After KEGG
enrichment of these DEGs, MAPK signaling pathway was
significantly enriched during vitellogenesis. Moreover,
the expression levels of genes involved in
carbohydrate, amino acid, and lipid metabolism were
found to be higher during vitellogenesis. The two genes
(Sp-Eip75B and Sp-Eip78C) that are homologous to the
vertebrate gene PPAR{\gamma} in the PPAR signaling
pathway, were identified. Additionally, genes in MAPK
signaling pathway might regulate lipid metabolism
through PPAR signaling pathway based on Protein-Protein
Interaction (PPI) network. These findings suggest that
MAPK signaling pathway plays a critical role in lipid
metabolism in the ovary during vitellogenesis, which
provides new insights into the mechanism of lipid
accumulation during ovarian maturation in mud crabs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wuertz:2023:TPC,
author = "Sven Wuertz and Filipa Be{\c{c}}a and Eva Kreuz and
Konrad M. Wanka and Rita Azeredo and Marina Machado and
Benjamin Costas",
title = "Two Probiotic Candidates of the Genus
\bioname{Psychrobacter} Modulate the Immune Response
and Disease Resistance after Experimental Infection in
Turbot (\bioname{Scophthalmus maximus}, {Linnaeus}
1758)",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "144",
day = "28",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030144",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/144",
abstract = "Probiotic bacteria are a recognized alternative to
classical methods of disease prophylaxis and therapy.
We tested the effects of their application on the
immune reaction in juvenile turbot. To prevent
digestion of the probiotics, rectal administration was
applied to maximise colonization, by-passing digestion
in the stomach. The application of Psychrobacter
nivimaris and Psychrobacter faecalis showed beneficial
effects on the inflammatory response and disease
resistance after infection with the common pathogen
Tenacibaculum maritimum. Treatment with P. nivimaris
and P. faecalis resulted in 0\% and 8\% mortalities
post-infection, while in the treatment control, an
elevated mortality of 20\% was observed. In the
challenge controls (no infection), no mortalities were
observed during the entire experimental period. After
an experimental infection, mRNA expression of selected
immune markers (mhc II {\alpha}, il-1{\beta}, tcr, tgf
{\beta} and tnf {\alpha}) were determined by RT-QPCR at
0, 1 and 5 days post-infection (dpi). At 0 dpi, gene
expression was comparable between the treatments and
the treatment control, suggesting that probiotics did
not act via immune stimulation of the host. At 1 dpi,
all genes were up-regulated in the treatment control
but not in the probiotic groups, indicating that the
infection in probiotic-treated fish developed at a less
severe level. At 5 dpi, mRNA expression returned to
baseline levels. As a conclusion, the native probiotic
candidates P. nivimaris and P. faecalis improved
survival, whereas, in the control, mortality increased
and expression of the immune markers was up-regulated
post infection. This highlights a potential application
of P. nivimaris and P. faecalis in disease prophylaxis,
but further research is needed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:DSS,
author = "Jianping Li and Chen Li and Congcong Li and Wei Luo
and Kang Wu and Songming Zhu and Zhangying Ye",
title = "Design and Study of a Spindle-Shaped Fry Head-to-Tail
Orientation Device",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "143",
day = "28",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030143",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/143",
abstract = "The head-to-tail orientation of spindle-shaped fry is
an important part of automated vaccine injection. The
fry's automatic orientation helps reduce labor and
improve efficiency during the automated injection. This
paper designed an electromagnetic vibration fry
orientation device for grass carp fry, measured its
friction characteristics with three different materials
in scales and against scales, analyzed the kinematic
characteristics of the fry on the electromagnetic
vibration feeder, and clarified the main factors
affecting fry transport. It used the trough's
inclination angle, amplitude, and material as the test
factors, whereas the orientation success rate and
orientation rate were used as the evaluation index.
Orthogonal combination tests were carried out with
90--150 mm grass carp fry as the research object to
obtain reasonable parameter combinations. The results
showed that the best orientation effect was achieved
when the surface of the trough was a silicone film with
many bumps, the inclination of the plate spring was
{\beta} = {15$^\circ $}, the inclination of the trough
plane was {\theta} y = {-4$^\circ $}, and the amplitude
A = 0.7 mm. In this parameter combination, the
directional device was tested and verified. The
targeted success rate was {\epsilon} = 95.5\%, and the
direction rate was {\eta} = 0.87 tail/s. The device
could meet the requirements of the head and tails
during the mechanized injection of the fry.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Miao:2023:IFQ,
author = "Xiaomin Miao and Hui Guo and Yong Song and Chunying Du
and Jingyun Feng and Yixi Tao and Hao Xu and Yun Li",
title = "Improvement of Flesh Quality of Farmed Silver Carp
(\bioname{Hypophthalmichthys molitrix}) by Short-Term
Stocked in Natural Water",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "142",
day = "28",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030142",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/142",
abstract = "To investigate the effects of different raising
environments on the flesh quality, the study set up
three groups of silver carp (Hypophthalmichthys
molitrix), that is, pond-farmed; short-term stocked;
and ranched, grown in a natural water ranch. This study
compared the differences in muscle proximate
composition and amino acid composition among three
groups. The results showed that there was no difference
between the ranched and the stocked groups in content
of crude protein, ash, total amino acids (TAA),
essential amino acids (EAA), and umami-flavored amino
acids (UAA), with both groups being significantly
higher than the farmed group. This shows that, in terms
of flesh quality, the stocked group was closer to the
ranched group and better than the farmed group after
being stocked in natural water for 30 days. The results
suggest that the flesh quality of farmed fish was
improved by short-term stocking in natural water. To
elucidate the reasons of flesh quality change in the
stocked group, this study compared the food composition
and intestinal digestive enzyme activity in three
groups, demonstrating that the ranched and stocked
groups had similar food composition, with their
detritus proportion lower than that of the farmed
group, consuming easily digestible phytoplankton
diatoms rich in amino acids and fatty acids. No
significant difference was seen between the ranched
group and the stocked group, for which amylase, lipase,
and trypsin activities were lower than that of the
farmed group. It is hypothesized that differences in
environmental and food composition resulted in lower
digestive enzyme activity in the ranched group and the
stocked group. In summary, the short-term stocking of
farmed fish in a natural water ranch can effectively
improve the nutrient content, quality and flavor, and
promote value of fish products.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Teixeira:2023:DTS,
author = "Cl{\'a}udia Teixeira and Rita Pedrosa and Carolina
Castro and Rui Magalh{\~a}es and Elisabete Matos and
Aires Oliva-Teles and Helena Peres and Amalia
P{\'e}rez-Jim{\'e}nez",
title = "Dietary Tryptophan Supplementation Implications on
Performance, Plasma Metabolites, and Amino Acid
Catabolism Enzymes in Meagre (\bioname{Argyrosomus
regius})",
journal = j-FISHES,
volume = "8",
number = "3",
pages = "141",
day = "28",
month = feb,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8030141",
ISSN = "2410-3888",
bibdate = "Thu Mar 30 09:07:32 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/3/141",
abstract = "Tryptophan (Trp) is an essential amino acid,
commercially available as a feed-grade product, and is
a precursor to serotonin and melatonin, which are both
important molecules in stress mitigation. Meagre have a
high potential for marine aquaculture diversification
but are highly susceptible to stressful conditions.
This study aimed to assess the potential role of
dietary tryptophan supplementation in meagre juveniles
in order to minimize the deleterious effect of
potential stress conditions. For this, a growth trial
was performed wherein meagre juveniles were fed four
isoproteic (45\%DM) and isolipidic (16\%DM) diets;
namely, a control diet, and three diets similar to the
control diet but supplemented with varying levels of
tryptophan, graded according to the resulting
percentage in each diet's dry matter (Control,
0.25\%Trp, 0.5\%Trp, and 1\%Trp), corresponding to a
total dietary tryptophan of 1.06, 1.70, 2.08, and 3.24
g 16 g {-1} N, respectively. Diets were tested in
triplicate, and fish were fed twice a day, six days a
week, for eight weeks. Five days after the end of the
growth trial, a time-course blood sampling was
performed at 0 h, 1 h, 3 h, 6 h, and 12 h after
feeding. At the 6 h sampling point, the liver was also
collected. Overall, our results indicate that 1Trp
supplementation (total dietary Trp 3.24 g 16 g {-1} N)
may be harmful to fish, decreasing growth performance
and feed utilization, although doses up to 0.5Trp do
not influence these parameters. Voluntary feed intake
lineally decreased with the increase in the level of
dietary Trp. Whole-body lipid content decreased at the
highest tryptophan inclusion, whereas no changes were
observed in protein levels. After 12 h from feeding,
plasma glucose levels were lower in all dietary
treatments supplemented with tryptophan compared to
those observed in the control. Hepatic enzyme activity
of protein catabolism decreased with dietary Trp
inclusion. Overall, our results suggested that while a
dietary Trp level increase of up to 2.08 g 16 g {-1} N
did not affect growth performance and feed efficiency,
both these parameters were severely compromised with a
Trp level of 3.24 g 16 g {-1} N.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fang:2023:ETS,
author = "Dan Fang and Jun Mei and Jing Xie and Weiqiang Qiu",
title = "The Effects of Transport Stress (Temperature and
Vibration) on Blood Biochemical Parameters, Oxidative
Stress, and Gill Histomorphology of Pearl Gentian
Groupers",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "218",
day = "21",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040218",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/218",
abstract = "The transport of living fish is an important part of
the fish farming process. The transport usually causes
fish stress. This study evaluated the effects of
transport temperature and vibration frequency on water
quality, blood biochemical parameters, gill
histomorphology, oxidative stress, and meat quality of
pearl gentian groupers after transport. First,
1-year-old groupers (450 {\pm} 25 g) were transported
in plastic bags for 48 h, including the following
treatments: no shaking, transported at 15 {$^\circ $}C,
shaking at 70 rpm, transported at 15 {$^\circ $}C (15
{$^\circ $}C/70 rpm); shaking at 120 rpm, transported
at 15 {$^\circ $}C (15 {$^\circ $}C/120 rpm); no
shaking, transported at 25 {$^\circ $}C; shaking at 70
rpm, transported at 25 {$^\circ $}C (25 {$^\circ $}C/70
rpm); and shaking at 120 rpm, transported at 25
{$^\circ $}C (25 {$^\circ $}C/120 rpm). Serum, liver,
gill, and muscle samples were collected for testing at
0, 12, 24, 36, and 48 h of exposure. During the 48 h
transport, total ammonia nitrogen (TAN), superoxide
dismutase (SOD), catalase (CAT), glutathione peroxidase
(GSH-PX), and malondialdehyde (MDA) were significantly
increased in the transport group compared to the
control group. In the early stage of transportation,
aspartate aminotransferase (AST), alanine
aminotransferase (ALT), cortisol (COR), lactate
dehydrogenase (LDH), and glucose (GLU) in the
transportation groups were significantly higher than
those in the control group, while the water quality pH
and dissolved oxygen (DO) levels decreased
significantly. Compared with untransported fish, the
total free amino acid (TFAA) content increased by
40.27\% and 31.74\% in the 25 {$^\circ $}C/70 rpm and
25 {$^\circ $}C/120 rpm groups, respectively. In
addition, the results of hematoxylin--eosin staining
and scanning electron microscopy showed that the
epithelial cells in the high-speed group were swollen,
the gill lamella was severely curved, and a large
amount of mucus was secreted. This study explores the
basic information of transportation, which will help to
select the conditions that are more suitable for the
successful transportation of pearl gentian groupers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kwikiriza:2023:MVN,
author = "Gerald Kwikiriza and Mourine J. Yegon and Nelson
Byamugisha and Apulnal Beingana and Faith Atukwatse and
Alex Barekye and Juliet K. Nattabi and Harald
Meimberg",
title = "Morphometric Variations of \geoname{Nile} Tilapia
(\bioname{Oreochromis niloticus}) ({Linnaeus}, 1758)
Local Strains Collected from Different Fish Farms in
\geoname{South Western Highland Agro-Ecological Zone
(SWHAEZ), Uganda}: Screening Strains for Aquaculture",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "217",
day = "20",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040217",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/217",
abstract = "Despite the global contribution of aquaculture as an
important pillar for food and nutritional security, its
rapid growth has been hampered by inadequate and
poor-quality seed due to inbreeding, hybridization of
related stocks, and poor-quality broodstock. Phenotypic
and genotypic characterization of Nile tilapia in
natural water bodies of Uganda has been studied but
research gaps remain on the morphometric
characterization of farmed O. niloticus in Uganda,
which hinders selective breeding initiatives. This
study characterized O. niloticus from various farmers
in Uganda's Southwestern Highland Agro-Ecological Zone
using their morphometric traits. In this study, 8
morphometric traits were taken on each of the 258 fish
samples collected with at least 30 samples sampled from
each pond system in the zone. The morphometric data
generated were analyzed using multivariate analysis. In
order to delineate the populations from the different
ponds, Discriminant Function analysis (DFA) was
performed on the dataset and distribution plots
generated to test on the purity of the strains. There
were significant differences in the morphometric traits
among the populations with the Victoria fish population
having the highest standard length while the Kyoga
population had the highest weight. Morphometric
characterization grouped the fish into three clusters,
indicative that fish farms in the zone possibly had
different sources of fish seed. The condition factor,
which is a measure of wellbeing of the fish, showed
that all the populations displayed an isometric growth
which is indicative of the fish growing in length as it
is in weight. The results of this study provide
evidence that local O. niloticus from different farms
display differences in body shape as a result of using
fish seed from different sources. This aspect can be
applied in selective breeding programs after
establishing the genetic structure of the farmed Nile
tilapia stock.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bonvechio:2023:SEF,
author = "Kimberly I. Bonvechio and Ramesh Paudyal and Chelsey
Crandall and Andrew K. Carlson",
title = "Survey Evaluation of \geoname{Florida}'s Freshwater
Fisheries Long-Term Monitoring Program",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "216",
day = "19",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040216",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/216",
abstract = "Natural resource monitoring programs benefit from
routine evaluation. Here, Florida's statewide
Freshwater Fisheries Long-Term Monitoring (LTM) program
is used to show how stakeholder surveys can be integral
to this process. In 2022, an online questionnaire was
sent to internal stakeholders, i.e., state agency
personnel who collect, enter, or use freshwater
fisheries data for fisheries and habitat management
purposes. The survey's primary objective was to
evaluate the program at its 15-year mark; secondary
objectives were to compare results with a similar
survey conducted at the 4-year mark, compare results
among respondents based on experience and functional
role, and develop recommendations for strategic
initiatives to further improve the program. The survey
consisted of 43 questions across six sections of
program evaluation: demographics; field sampling; data
entry, summary, and reporting; management decision
support; programmatic views; and additional input.
Respondents generally had positive views of the LTM
program, but the survey revealed differences among
respondents with different functional roles (e.g.,
fisheries researchers and managers viewed the
decisional value, priority, and sample sizes of LTM
data more favorably than habitat managers) while
highlighting high-priority future initiatives (e.g.,
database development). Our results demonstrate the
utility of stakeholder surveys as an important step in
evaluating monitoring programs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2023:SES,
author = "Xiaochen Liu and Daixia Wang and Yan Shang and Xuee Yu
and Baoquan Gao and Jianjian Lv and Jitao Li and Ping
Liu and Jian Li and Xianliang Meng",
title = "Survival, Energy Status, and Cellular Stress Responses
of the Juvenile Swimming Crab \bioname{Portunus
trituberculatus} under Acute Nitrite Stress",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "215",
day = "19",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040215",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/215",
abstract = "Nitrite is a common pollutant encountered in
aquaculture systems. During intensive hatchery,
accumulation of nitrite can cause massive mortality of
juvenile crustaceans. However, the nitrite toxicity and
cellular stress responses in juvenile crustaceans is
not clearly understood. Here, we investigate the
survival, energy metabolism, and cellular stress
responses in juvenile P. trituberculatus, an important
aquaculture species in China, under acute nitrite
stress. The results revealed nitrite resulted in a
significant decrease in survival rate of juvenile
swimming crab. After nitrite exposure, the activity of
catabolic enzymes, such as HK, PK, CS, and CPT-1, were
initially enhanced, and then they showed significant
decrease at the late stage of exposure, accompanied by
reduction in ATP and adenylate energy charge (AEC). The
impaired energy homeostasis was possibly associated
with disturbed AMPK signaling and enhanced anaerobic
metabolism, which was indicated by the high levels of
LDH activity and HIF-1{\alpha} expression. Furthermore,
we found that nitrite stress can depress antioxidant
systems and unfold protein responses, causing oxidative
damage and endoplasmic reticulum (ER) stress, and this,
in turn, can trigger autophagy and apoptosis through
both caspase-dependent and caspase-independent
pathways. The results of the present study improve our
understanding regarding adverse effects of nitrite on
P. trituberculatus and provide valuable information for
hatchery management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hamilton:2023:EIS,
author = "Erin F. Hamilton and Collin L. Juurakko and Katja
Engel and Josh D. Neufeld and John M. Casselman and
Charles W. Greer and Virginia K. Walker",
title = "Environmental Impacts on Skin Microbiomes of Sympatric
High \geoname{Arctic} Salmonids",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "214",
day = "18",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040214",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/214",
abstract = "In the region of King William Island, Nunavut, in the
Canadian high Arctic, populations of salmonids
including Arctic char (Salvelinus alpinus), cisco
(Coregonus autumnalis and C. sardinella) as well as
lake whitefish (C. clupeaformis) are diadromous,
overwintering in freshwater and transitioning to saline
waters following ice melt. Since these fish were
sampled at the same time and from the same traditional
fishing sites, comparison of their skin structures, as
revealed by 16S rRNA gene sequencing, has allowed an
assessment of influences on wild fish bacterial
communities. Arctic char skin microbiota underwent
turnover in different seasonal habitats, but these
striking differences in dispersion and diversity
metrics, as well as prominent taxa involving primarily
Proteobacteria and Firmicutes, were less apparent in
the sympatric salmonids. Not only do these results
refute the hypothesis that skin communities, for the
most part, reflect water microbiota, but they also
indicate that differential recruitment of bacteria is
influenced by the host genome and physiology. In
comparison to the well-adapted Arctic char, lake
whitefish at the northern edge of their range may be
particularly vulnerable, and we suggest the use of skin
microbiomes as a supplemental tool to monitor a
sustainable Indigenous salmonid harvest during this
period of change in the high Arctic.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Verhille:2023:ESR,
author = "Christine E. Verhille and Anthony P. Farrell",
title = "Endurance Swimming Is Related to Summer Lake Survival
of Rainbow Trout in a Warm Lake with Avian Piscivores",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "213",
day = "18",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040213",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/213",
abstract = "Fitness of fish is assumed to be influenced by
locomotion performance, but empirical evidence linking
swimming capacity to survival in nature remains sparse.
Poor triploid (3N) fish aerobic swimming performance in
conjunction with production of sibling diploid (2N) and
3N populations of genetically identical origin to
minimize variability among compared populations make 3N
trout an informative system to test hypotheses about
fitness consequences of fish locomotion. Here, we ask
if reduced survival of 3N relative to 2N trout in
natural ecosystems during periods of high temperature
relate to a lower aerobic swimming capacity and aerobic
scope of 3N compared with 2N conspecifics. Three-yearly
cohorts of conspecific 2N and 3N hatchery-reared,
yearling rainbow trout were ranked for swimming
endurance, externally marked for their endurance
quantile, and then stocked into two lakes as yearlings
to quantify their survival in the wild over summer as a
function of ploidy, temperature and endurance; all
while tracking temperature and depth habitat
utilization via telemetry. As expected, 3N swimming
endurance was lower than that of 2N, but with
considerable individual overlap. Aerobic swimming
endurance, especially for 3N, was predictive of summer
survival in a warm lake where piscivorous birds
potentially exerted high predation pressure, resulting
in low fish survival. This empirical evidence of a
connection between swimming endurance and fitness
provides support for long held assumptions of this
relationship and could inform future sport fishing
stocking industry practices to match fish strains to
ideal habitats.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez:2023:EPT,
author = "Enrique Pino Martinez and Albert Kjartan Dagbjartarson
Imsland and Anne-Camilla Diesen Hosfeld and Sigurd Olav
Handeland",
title = "Effect of Photoperiod and Transfer Time on
\geoname{Atlantic} Salmon Smolt Quality and Growth in
Freshwater and Seawater Aquaculture Systems",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "212",
day = "18",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040212",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/212",
abstract = "Smoltification is a key process in Atlantic salmon
aquaculture, given it prepares the fish for a
successful transit from fresh to seawater. However,
industry players have not yet reached a consensus on
the best protocols to produce high-quality smolts. In
this study, we assessed how the combination of two
photoperiod regimes in freshwater (continuous light or
LL, and natural photoperiod or LDN) and four transfer
times to seawater (February, March, April, and May)
affected smolt development and their subsequent growth
in seawater until slaughter during commercial
production. The results demonstrated that
smoltification and growth in freshwater were only
slightly modulated by the photoperiod treatment and
were instead much more affected by the limiting effect
of the low water temperature during that period. In
seawater, the growth rate was the highest in the same
groups, which had, however, experienced a delay in
growth when in freshwater, and consequently, no
differences in the final body weight between the eight
treatments were found. Such compensatory growth in the
sea was probably enhanced by the increasing smolt
quality, which could allow for better performance in
seawater. A significant link between the weight at
slaughter and weight at transfer was observed only in
the groups with a lower smolt quality (LL-Feb, LDN-Feb
and LDN-Mar), which suggests that larger individuals
could cope better with a saline environment. In
contrast, smaller smolts probably suffered greater
osmotic stress that hindered their performance at sea.
Afterwards, as smolt quality increased in the
subsequent transfer groups, the relevance of this size
effect decreased. This means that the industry may
benefit from transferring larger smolts to seawater,
especially if these are suspected of having developed
suboptimal seawater tolerance. Those individuals are
likely to cope better with saline conditions than
smaller smolts. Future research should focus on the
possible long-term effects of freshwater-rearing
regimes on smolt performance in the seawater phase.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2023:FHS,
author = "Di Wu and Hao Feng and Ying Zou and Juan Xiao and
Pengfei Zhang and Yuxiang Ji and Sovan Lek and Zhiqiang
Guo and Qiongyao Fu",
title = "Feeding Habit-Specific Heavy Metal Bioaccumulation and
Health Risk Assessment of Fish in a Tropical Reservoir
in \geoname{Southern China}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "211",
day = "18",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040211",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/211",
abstract = "Dietary uptake is well known as the predominant
pathway of heavy metal bioaccumulation in organisms.
Our study used a typical tropical reservoir and fish as
a modeling system to test the hypothesis that feeding
habits and living habitats significantly affect heavy
metal bioaccumulation in fish. Specifically, Cr, Mn,
Fe, Ni, Cu, Zn, Cd, and Pb concentrations in water,
sediment, and fish, and {\delta} 13 C and {\delta} 15 N
in 13 fish species were detected in the Songtao
Reservoir of Hainan Province, southern China. Our
results indicated that Zn concentration in carnivorous
fish was higher than in omnivorous fish. Principal
components analysis visually differentiated pelagic,
benthopelagic, and demersal fish groups. Moreover, we
found that the fish feeding in the demersal habitat
showed higher heavy metal levels than those in the
pelagic habitat. Additionally, the heavy metal contents
in demersal fish were significantly positively
correlated with sediments, while no positive
correlation was observed in pelagic-feeding fish. The
{\delta} 15 N and the concentration of Ni, Zn in fish
had a significantly positive correlation, suggesting
the potential biomagnification. In contrast, Ni, Fe,
Cu, and Cd negatively correlated with fish body
weight/length, indicating the growth dilution effects.
Finally, the estimated daily intake (EDI) of the metals
was far below the provisional tolerable daily intake
(PTDI), and target hazard quotients (THQ) were
{$<$1}.0, indicating that the fish had no risk for
consumption risks. Overall, our finding partially
validated the hypothesis that the feeding habits and
living habitats significantly influence heavy metal
bioaccumulation in fish, which might be a broad
generality for metal exposure scenarios in aquatic
environments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nguyen:2023:EMG,
author = "Luong Trong Nguyen and Khanh Quoc Nguyen and Toan Phi
Nguyen",
title = "Experimental Mixed Gillnets Improve Catches of
Narrow-Barred \geoname{Spanish} Mackerel
(\bioname{Scomberomorus commerson})",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "210",
day = "18",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040210",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/210",
abstract = "A new gillnet made from multiple mesh sizes ranging
from 125 to 180 mm of stretched mesh (experimental
gillnet) was tested under commercial fishing conditions
to compare the fishing performance with that of
conventional gillnets with a 125 mm mesh opening
(control gillnet). Catch efficiency and size
selectivity between the two gillnet types were
evaluated throughout one year of fishing in three
different locations in the waters of Vietnam.
Experimental gillnets caught narrow-barred Spanish
mackerel (Scomberomorus commerson), spotted mackerel
(Scomberomorus guttatus), and wahoo (Acanthocybium
solandri) in comparable amounts to the control
gillnets, with the moon phase, month, and depth
explaining some of the variation in the catch per unit
effort (CPUE). An analysis of the size-dependent catch
comparison rates and selectivity parameters showed that
the experimental gillnets captured a wider range of
narrow-barred Spanish mackerel sizes, but with a
substantial proportion of individuals larger than those
caught by the control gillnets. This is of higher
weight per unit effort, and fishing enterprises
therefore could improve their economic benefits by
using modified gillnets with multiple mesh sizes. Our
findings also support the biological and environmental
benefits of the modified gillnet size selection, which
might also extend to other species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wan:2023:UEM,
author = "Rong Wan and Pengbo Song and Zengguang Li and Xiangyu
Long and Dong Wang and Lu Zhai",
title = "Use of Ensemble Model for Modeling the Larval Fish
Habitats of Different Ecological Guilds in the
\geoname{Yangtze Estuary}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "209",
day = "17",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040209",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/209",
abstract = "Estuaries provide nursery habitats for a variety of
fish species of different ecological guilds and have
complicated environmental conditions. In this study, we
applied ensemble species distribution models (SDMs) to
four abundant and different ecological guild larval
fish species (Hemiculter bleekeri (freshwater guild),
Pseudolaubuca sinensis (freshwater guild), Coilia
mystus (brackish water guild), and Engraulis japonicus
(marine guild)) to explore their suitable habitats and
the fish--habitat relationships in the Yangtze Estuary.
The results showed that random forest (RF) demonstrated
the best performance in all single algorithms and the
surface range envelop (SRE) model was scarcely
accurate. The ensemble SDMs demonstrated a superior
predictive ability compared with any single algorithm,
with the true skill statistic (TSS) and the area under
the receiver operating characteristic curve (AUC)
scores being above 0.899 and 0.641, respectively.
Binary presence--absence maps showed the different
spatial distribution patterns of the four species. We
primarily found the freshwater species (P. sinensis and
H. bleekeri) present in the South Branch (west of
{122$^\circ $} E), whereas we found the marine species
(E. japonicus) anywhere except inside the South Branch
(west of {121.8$^\circ $} E). The area for P. sinensis
(1615.93 km 2) was relatively larger than that for H.
bleekeri (1136.87 km 2). We predicted that the brackish
water species (C. mystus) would most likely be present
inside the North Branch (west of {122$^\circ $} E),
Eastern Chongming, and outside the South Branch (east
of {121.8$^\circ $} E). Salinity, as a key
environmental variable, contributed to the spatial
variability. A low salinity (sea surface salinity (SSS)
{$<$} 3) was beneficial for P. sinensis and H. bleekeri
but was not suitable for E. japonicus. The SSS suitable
range for C. mystus was 5--10 and 12--20. Multiple
ecological guild species dwelled in the confluence of
salt and fresh water. Our results will play an
important role in the design of specific conservation
strategies for fishery resources in this area.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Eissa:2023:DEN,
author = "El-Sayed Hemdan Eissa and Bothaina A. Alaidaroos and
Samyah D. Jastaniah and Mohammad Bodrul Munir and Manal
E. Shafi and Yasmin M. Abd El-Aziz and Walaa K. Bazina
and Saadiah binti Ibrahim and Moaheda E. H. Eissa and
Marina Paolucci and Fatima S. Alaryani and Nadia N. B.
Abd El-Hamed and Mohamed E. Abd El-Hack and Saadea
Saadony",
title = "Dietary Effects of Nano Curcumin on Growth
Performances, Body Composition, Blood Parameters and
Histopathological Alternation in Red Tilapia
(\bioname{Oreochromis} sp.) Challenged with
\bioname{Aspergillus flavus}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "208",
day = "17",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040208",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/208",
abstract = "This research examined the role of nano curcumin (NC)
on growth performances, body composition, and blood
parameters of red tilapia (Oreochromis sp.) challenged
with Aspergillus flavus. Fish (5.0 g {\pm} 0.30) were
randomly distributed in four equal groups (20 fish per
pond in triplicates) and fed various concentrations of
NC fortified with 0 (Control), 40 mg/kg (NC1), 50 mg/kg
(NC2), and 60 mg/kg diet (NC3) of nano curcumin. After
eight weeks of the feeding trial, the fish were
challenged with A. flavus for 15 days, and the
cumulative mortality was recorded. Fish fed with
different concentrations of NC improved significantly
(p {$<$} 0.05) the growth performances, feed
utilization, and survival rate. There was no
significant (p {$>$} 0.05) difference between NC2 and
NC3 treatments. However, NC3 exhibited higher
performances. Fish feed supplemented with NC decreased
the mortality rate when challenged with A. flavus.
Hence, dietary supplementation of NC enhanced the
growth and health status of Oreochromis sp. and
protected it from A. flavus infection. This study
suggests the optimum inclusion level of NC is a 50--60
mg/kg diet.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mendez-Tepepa:2023:CEA,
author = "Maribel M{\'e}ndez-Tepepa and Karla
Hern{\'a}ndez-P{\'e}rez and Libertad
Ju{\'a}rez-Santacruz and Senobia Rosalia Cruz-Lumbreras
and Edelmira Garc{\'\i}a-Nieto and Arely
Anaya-Hern{\'a}ndez and Cuauht{\'e}moc Morales-Cruz",
title = "Cytotoxic Effects of the Atrazine Herbicide on
Erythrocytes and Liver Damage in \bioname{Lithobates
spectabilis}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "207",
day = "16",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040207",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/207",
abstract = "In Mexico, atrazine is one of the herbicides most
widely authorized and used in different irrigation
districts. Atrazine is a herbicide that contaminates
aquatic systems. Previous studies have shown that
atrazine causes damage to red blood cells and liver
tissue in different aquatic species, including
abnormalities in melanomacrophages. However, more
information on amphibians is needed, since most of
these studies have been done on fish. Furthermore, no
study has determined the effect of atrazine on species
native to Mexico. Therefore, in this study, we asked
what the effects of atrazine are on the erythrocytes
and melanomacrophages of the liver in the male frog
(Lithobates spectabilis). In the present study, we
analyzed (1) the cytotoxicity of atrazine using the
micronucleus test, (2) the area of melanomacrophage
centers and the presence of melanin, and (3) the
characterization of liver damage using histological
techniques. Our results show that atrazine is cytotoxic
to erythrocytes, increases the area of and melanin
presence in melanomacrophage centers, and causes liver
damage in male L. spectabilis. Therefore,
hepatotoxicity and cytotoxicity are indicators of
environmental stress. We suggest monitoring Mexico's
aquatic systems and further analyzing atrazine effects
and other pollutants on native species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Costa:2023:MPT,
author = "Wilson J. E. M. Costa and Valter M. Azevedo-Santos and
Jos{\'e} Leonardo O. Mattos and Axel M. Katz",
title = "Molecular Phylogeny, Taxonomy and Distribution
Patterns of Trichomycterine Catfishes in the
\geoname{Middle Rio Grande Drainage, South-Eastern
Brazil} ({Siluriformes: Trichomycteridae})",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "206",
day = "15",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040206",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/206",
abstract = "The Rio Grande drainage plays a key role in supplying
water and electricity to large urban centres, but some
components of its rich ichthyofauna are still poorly
known. Based on our field inventories in the middle
section of the drainage, we recognised 10
trichomycterine endemic species, of which 6 species are
new and described herein. A molecular analysis (2600 bp
for 43 taxa) indicated that the species of both
subgenera do not form monophyletic groups. One species
of the subgenus Cryptocambeva is closely related to
species from the inner Brazilian Plateau, whereas other
species of this subgenus are closely related to species
endemic to smaller coastal basins. The species of the
subgenus Paracambeva belong to different lineages of a
clade endemic to the Rio Grande drainage. These species
are diagnosed by the characters of their external
morphology and osteology. A key to species
identification is provided. The species distribution
patterns support delimitation of three areas of
endemism, which may have a relevant role for proposals
of conservation strategies: the Uberaba, the S{\~a}o
Jo{\~a}o-Sapuca{\'\i}, and the Tamborete areas. The
last one, a small area confined between the Serra da
Canastra and the Rio Grande at the Furnas dam, is
particularly important for sheltering three endemic
trichomycterines and two loricariid catfishes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Burbank:2023:TVB,
author = "Jacob Burbank and Jenni L. McDermid and Fran{\c{c}}ois
Turcotte and Nicolas Rolland",
title = "Temporal Variation in {Von Bertalanffy} Growth Curves
and Generation Time of \geoname{Southern Gulf of St.
Lawrence} Spring and Fall Spawning \geoname{Atlantic}
Herring (\bioname{Clupea harengus})",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "205",
day = "15",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040205",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/205",
abstract = "Examining temporal changes in the growth and
generation time of fish species can be valuable for
understanding population responses to different
management measures and environmental conditions.
Atlantic herring (Clupea harengus -Linnaeus, 1758) is
an ecologically and commercially important pelagic
forage fish species that occupies the southern Gulf of
St. Lawrence. Here, we developed von Bertalanffy growth
curves and estimated yearly values of generation time
for NAFO Division 4TVn Atlantic herring for the years
from 1988 to 2021. The results indicate a temporal
reduction in the growth and generation time of both
spring and fall spawning herring. Over the time series,
the generation time of spring and fall spawners reduced
by approximately 1 and 2 years, respectively.
Furthermore, the average generation time of spring
spawners across the time series (6.23 years (95\% CI:
5.78--6.85 years)) was approximately 1 year lower than
that of fall spawners (7.52 years (6.82--8.34 years)).
Overall, the findings of this study can be used to
better inform stock assessments and rebuilding plans
for 4TVn spring and fall spawning Atlantic herring and
highlight the importance of examining temporal trends
in growth and generation time for more effective
management of fish stocks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:EIH,
author = "Xinxin Wang and Junyi Xie and Yan Luo and Xiao Wang
and Gaobo Guo and Xinxing You",
title = "Experimental Investigation of the Hydrodynamic
Characteristics of Longline Aquaculture Facilities
under Current and Wave Conditions",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "204",
day = "15",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040204",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/204",
abstract = "In this study, a longline aquaculture facility with
lantern nets off the coast of northern China was
modelled to conduct hydrodynamic tests starting from
the culture unit to the entire facility under various
current and wave conditions. The experimental results
indicated that the drag coefficients of the lantern net
model with weights of 0.5, 0.75, and 1.0 kg were 0.75,
0.83, and 0.91, respectively, in the Reynolds number
range of 1 $ \times $ 10 4 --1 $ \times $ 10 6. The
current-driven upstream mooring line was more dominant
than the wave-driven tension, and a simplified model of
the longline facility accurately predicted the mooring
line tension under the current conditions. The scope of
the mooring line (defined as the length of the mooring
line related to the water depth) played an important
role in eliminating an order of magnitude difference in
mooring tension under the wave conditions. The
amplitudes of the vertical movement of the longline
facility were smaller than the wave height when L / L m
was less than 1.5. Therefore, detailed information is
needed to better understand the hydrodynamic
characteristics and motion response of longline
aquaculture facilities for the safe operation of
longline structures in offshore environments, in order
to process high-quality oyster products.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Saavedra:2023:TPP,
author = "Carlos Saavedra and Massimo Milan and Ricardo B. Leite
and David Cordero and Tomaso Patarnello and M. Leonor
Cancela and Luca Bargelloni",
title = "Transcriptional Profiling of Populations in the Clam
\bioname{Ruditapes decussatus} Suggests Genetically
Determined Differentiation in Gene Expression along
Parallel Temperature Gradients and between Races of the
\geoname{Atlantic Ocean} and \geoname{West
Mediterranean Sea}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "203",
day = "14",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040203",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/203",
abstract = "Ongoing ocean warming due to climate change poses new
challenges for marine life and its exploitation. We
have used transcriptomics to find genetically based
responses to increased temperature in natural
populations of the marine clam Ruditapes decussatus,
which lives along parallel thermal gradients in
southern Europe. Clams of the Atlantic and West
Mediterranean races were collected in northern (cool)
and a southern (warm) localities. The animals were kept
in running seawater in the warm, southern Atlantic
locality for a 15-week period. During this period,
water temperature was raised to typical southern
European summer values. After this period, an
expression profile was obtained for a total of 34 clams
and 11,025 probes by means of an oligonucleotide
microarray. We found distinct transcriptional patterns
for each population based on a total of 552
differentially expressed genes (DEGs), indicating
innate differences which probably have a genetic basis.
Race and latitude contributed significantly to gene
expression differences, with very different sets of
DEGs. A gene ontology analysis showed that races
differed mainly in the genes involved in ribosomal
function and protein biosynthesis, while genes related
to glutathione metabolism and ATP synthesis in the
mitochondria were the most outstanding with respect to
north/south transcriptional differences.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Basilone:2023:AGE,
author = "Gualtiero Basilone and Rosalia Ferreri and Angelo
Bonanno and Simona Genovese and Marco Barra and
Salvatore Aronica",
title = "Age and Growth of \geoname{European} Sardine
(\bioname{Sardina pilchardus}) in the \geoname{Central
Mediterranean Sea}: Implication for Stock Assessment",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "202",
day = "13",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040202",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/202",
abstract = "Understanding the drivers of fish growth is essential
for predicting productivity, stability, and resilience
of exploited populations. For the European sardine
(Sardina pilchardus) in the Strait of Sicily (Central
Mediterranean Sea, GSA16), growth parameters or length
at age estimates have never been published before. To
fill this data gap, the length and age as well as the
von Bertalanffy parameters (the most widely used growth
model) were estimated. Data from landing samples during
the period 2009--2019 were collected by two methods
(purse seine and mid-water pelagic trawl). Temporal
trends in average length at age, as well as an overall
age-length key were obtained and compared with other
areas across the geographical distribution range of
sardine in the Mediterranean Sea. The observed age
range was 0--3 years with most of the individuals
belonging to Age 1 (52\%) and Age 2 (43\%). The mean
length at age, for the entire study period, was 11.7
({\pm}0.08) cm for Age 0; 13.4 ({\pm}0.09) cm for Age
1; 15 ({\pm}0.1) cm for Age 2; and 16.6 ({\pm}0.11) cm
for Age 3. Furthermore, during the considered period, a
reduction in the length at age was observed in the
older classes (Age 2 and Age 3). The estimated
parameters of the von Bertalanffy growth model were L
inf = 18 ({\pm}1.15) cm, K= 0.459 ({\pm}0.018), and t 0
= {$-$1}.99 ({\pm}0.008) and, accordingly, the
mortality vector was obtained according to Gislason's
model, for each age class, these values were 0.99
(0.98--1.02) C at Age 0; 0.71 (0.7--0.73) y {$-$1} at
Age 1; 0.6 (0.59--0.62) y {$-$1} at Age 2; 0.54
(0.53--0.56) y {$-$1} at Age 3; and 0.51 (0.49--0.53) y
{$-$1} at Age 4. Results appeared in agreement with
literature from other areas of the Mediterranean Sea
and suggested a poor condition status of the sardine
stock in the GSA16.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:PRP,
author = "Lin Chen and Xiaoyu Zhang and Huanzhang Liu",
title = "Phylogenetic Relationships of the Pseudogobionini
Group ({Teleostei: Cyprinidae}) with Selection Pressure
Analyses to Genes of Mitochondrial Genome",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "201",
day = "13",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040201",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/201",
abstract = "We newly sequenced complete mitochondrial genomes
(mitogenome) of two gudgeon species Saurogobio dabryi
and S. punctatus, and we downloaded 49 gudgeon
mitogenomes from GenBank to investigate the phylogeny
of the Pseudogobionini group and analyze selection
pressure of the genes. With genera Gobio, Acanthogobio,
and Romanogobio as outgroups, the phylogeny of the
Pseudogobionini group was revealed as ((Xenophysogobio
+ Gobiobotia) + (Saurogobio + (Abbottina + (Pseudogobio
+ Biwia complex)))) based on the concatenated
nucleotide sequences of 13 protein-coding genes (PCGs).
Based on the molecular phylogeny and morphological or
osteological characters, we proposed a classification
system of the Pseudogobionini group. Moreover, five
pairs of sister taxa were selected for gene selection
pressure analyses to explore the link of mitochondrial
gene evolution to group differentiation and
adaptations. We detected significantly different dN/dS
values in 11 out of 13 (excluding ND3 and ND4L) PCGs in
five pairs of clades, significantly different mean
dN/dS, dN, and/or dS values in 8 out of 13 PCGs
(excluding ND2, ATP8, ND3, ND4L, and ND6) in three
pairs of sub-clades and seven positively selected sites
in another three pairs of sub-clades. These results
indicated that mitochondrial gene evolution might have
contributed to group differentiation and adaptations
especially for river or lake environments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Toledo:2023:RBJ,
author = "Cesar Toledo and Eduardo Rubilar and Lorena Marchant
and Jessica D{\"o}rner and Lorenzo M{\'a}rquez and
V{\'\i}ctor Mart{\'\i}nez and Patricio Dantagnan",
title = "Relationship between Jaw Malformations and Long-Chain
{PUFA}'s in \bioname{Seriola lalandi} Larvae during the
Spawning Season at a Commercial Hatchery",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "200",
day = "13",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040200",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/200",
abstract = "Jaw malformations imply an important problem during
the commercial production of Seriola lalandi larvae and
juvenile fish in Chile and New Zealand. Since the rate
of malformations in other fish species has been
associated with the content of long-chain PUFA
(LC-PUFA) in neutral lipids of the diet, the
relationship between body LC-PUFA and the rate of
malformations, together with a transcriptomic analysis
of genes related to the metabolism and transport of
lipids in commercially produced S. lalandi larvae have
been investigated in the present work. A total of 10
batches of S. lalandi larvae were obtained and cultured
at about 22 {$^\circ $}C following the protocols of a
Chilean commercial hatchery during the spawning season.
There were three larval batches that were collected in
August (austral winter), three batches that were
obtained in October (spring), and finally four spawning
events that were obtained in December-January
(spring-summer). The rate and type of jaw
malformations, the profile of long-chain PUFA (LC-PUFA)
of total lipids during larval culture, and the
distribution of LC-PUFA were analyzed. Additionally, a
transcriptome analysis related to lipid metabolism in
40-day-old larvae was performed. As a result, a
decrease in the rate of malformations was recorded from
August to December. DHA showed a steep decrease between
days 1 and 10 of culture, probably due to the
consumption of yolk sac lipids, but a higher
proportional change was noted in larvae that hatched
during August. The DHA content in polar lipids (PL) and
neutral lipids (NL) of 40-day-old larvae increased from
August to December, so that the abundance of DHA was
higher in PL in larvae that hatched in December, but it
was higher in NL in those larvae that hatched in
October. In conclusion, the rate of jaw malformations
was associated with the rapid decline in DHA during
early larval life and the highest abundance of DHA in
neutral lipids at the end of the larval life.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rahman:2023:ISA,
author = "Afaf N. Abdel Rahman and Sameh H. Ismail and Moustafa
M. S. Fouda and Abdelwahab A. Abdelwarith and Elsayed
M. Younis and Samah S. Khalil and Mahmoud M. El-Saber
and Ahmed E. Abdelhamid and Simon J. Davies and Rowida
E. Ibrahim",
title = "Impact of \bioname{Streptococcus agalactiae} Challenge
on Immune Response, Antioxidant Status and Hepatorenal
Indices of \geoname{Nile} Tilapia: The Palliative Role
of Chitosan White Poplar Nanocapsule",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "199",
day = "12",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040199",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/199",
abstract = "A new insight into the synthesis of the herbal plant
(White poplar, Poplus alba) leave extract using
chitosan nanocapsule was studied. The in vitro
antibacterial activity of chitosan white poplar
nanocapsule (CWPNC) against Streptococcus agalactiae
(S. agalactiae) was determined. About 120 fish were
categorized for 7 days into four groups. The first and
second (CWPNC) groups were treated with 0 mg/L and 3
mg/L CWPNC in the water, respectively, without being
challenged; the first group was a control. The third
(S. agalactiae) and fourth (CWPNC + S. agalactiae)
groups were treated with 0 and 3 mg/L CWPNC,
respectively, and challenged with S. agalactiae (0.5 $
\times $ 10 7 CFU/mL). The obtained results revealed
that CWPNC had an in vitro antibacterial activity
against S. agalactiae. Moreover, S. agalactiae
infection caused a significant elevation (p {$<$} 0.05)
in the lipid peroxidation (malondialdehyde) and
hepatorenal biomarkers, as well as the lowest
significant (p {$<$} 0.05) survival rate (33.33\%).
Moreover, a significant depletion (p {$<$} 0.05) in the
level of antioxidants (catalase and superoxide
dismutase) and the immune indicators (immunoglobulin,
lysozyme activity, and complement 3) were the
consequences of S. agalactiae infection. Treatment of
the infected fish with 3 mg/L CWPNC alleviated these
bad circumstances.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shen:2023:IEA,
author = "Huihui Shen and Liming Song",
title = "Implementing Ecosystem Approach to Fisheries
Management in the {Western and Central Pacific
Fisheries Commission}: Challenges and Prospects",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "198",
day = "12",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040198",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/198",
abstract = "The ecosystem approach to fisheries management (EAFM)
is considered one of the key management approaches for
addressing global resource decline and promoting the
health and resilience of ecosystems. This paper
explores how the Western and Central Pacific Fisheries
Commission (WCPFC), which manages tuna fisheries, has
incorporated the ecosystem approach into its management
and decision-making system. This study finds that (1)
the WCPFC lacks incentives to adopt EAFM as a whole due
to its management priorities on target species and some
key bycatch species; (2) inadequate scientific
information on associated species and the environment
hinders ecosystem risk assessments, leading to delays
in EAFM-related decisions; and (3) the organization has
given little consideration to human factors. The
authors suggest developing an EAFM vision with a clear
roadmap to guide the implementation of EAFM and
applying area-based management tools in cases where
there is limited data and scientific information. The
proposed coordination mechanism aims to address growing
concerns about labor issues by involving multiple
stakeholders in the development of a management measure
on labor standards.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Youn:2023:AGC,
author = "Byoung-Il Youn and Dong-Hyuk Choi and Tae-Hyoung Roh
and Seung-Hwan Lee and Kyeong-Ho Han and Dae-Hyeon Kwon
and Maeng-Jin Kim",
title = "Age and Growth Characteristics of \bioname{Okamejei
kenojei} in the \geoname{West Sea of South Korea}
According to Coronal Vertebral Microstructure",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "197",
day = "10",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040197",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/197",
abstract = "In this study, the growth and age characteristics of
Okamejei kenojei in the West Sea of South Korea were
examined, and specimens were collected by bottom trawls
from January to December 2019. The relationship between
disc width (L) and body weight (W) was $ W = 6.1 \times
10^{-3} $ L 3.40. Age was determined by measuring
annuli on the vertebral centrum surface, which forms
around June every year. The spawning period was
extrapolated from monthly changes of the gonadosomatic
index in June--July. The relationship between the
vertebral centrum radius and disc width, which was
analyzed separately for females and males, was
determined as L = 16.159 R 0.6145 and L = 15.543 R
0.6851, respectively. Finally, O. kenojei growth, that
is, the disc width (L) at a certain age (t), was
expressed using the von Bertalanffy growth equation as
L t = 58.70(1 {$-$} e {$-$0}.21(t +0.12)) for females
and L t = 53.94(1 {$-$} e {$-$0}.26(t +0.05)) for
males. This study provides basic data on the age
characteristics of O. kenojei for future research and
more efficient fish stock management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:MAM,
author = "Xi-Lian Li and Pei-Jing Shen and Wen-Ping Jiang and
Ji-Lun Meng and Hai-Hua Cheng and Qiang Gao",
title = "Metabonomic Analysis of \bioname{Macrobrachium
rosenbergii} with {Iron Prawn Syndrome (IPS)}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "196",
day = "09",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040196",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/196",
abstract = "We previously reported on the comparison of proteomic
data between seven tissue types of a novel ``iron
prawn'' species. However, no transcripts or metabolic
information are available for this species. We
therefore performed shotgun LC--MS/MS metabonomic and
RNA-seq analyses of the total protein from ``iron
prawns''. KEGG analysis revealed that the largest group
consisted of a total of 114 KEGG pathway proteins,
comparing the ``iron prawns'' with the normal prawns. A
total of 423 peptides, corresponding to metabolic
pathways, ABC transporters, starch and sucrose
metabolism, insulin resistance/secretion, fatty
digestion and absorption, and lipid metabolism, were
identified. The pathways of carbohydrate and amino acid
metabolism decreased in female iron prawns, while
organic acid and its derivatives increased. However,
the pathway of organic acid and its derivatives
decreased and lipid metabolism increased in the male
iron prawns. The pathways of choline metabolism in
cancer and glycerophospholipid/histidine/propanoate
metabolism have been significantly affected in iron
prawns. Our work provides insight into the
understanding of the formation mechanism of the ``iron
prawn''.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mokhtar:2023:GMF,
author = "Doaa M. Mokhtar and Marwa M. Hussein and Giacomo
Zaccone and Alessio Alesci and Eugenia Rita Lauriano
and Ramy K. A. Sayed",
title = "Gills of Molly Fish: a Potential Role in Neuro-Immune
Interaction",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "195",
day = "08",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040195",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/195",
abstract = "This study identified the cellular compositions of the
gills in molly fish and their role in immunity using
light-, electron- microscopy, and immunohistochemistry.
The molly fish gills consisted of four holobranchs
spaced between five branchial slits. Each hemibranch
carried many fine primary and secondary gill lamellae.
The gill arch was a curved cartilaginous structure,
from which radiated the bony supports of the primary
lamellae. The gill arch contained the afferent and
efferent brachial arteries. The gill arch was covered
by epidermal tissue rich with mucous cells. The primary
lamella had a central cartilaginous support and
efferent and afferent arterioles and was covered with
pavement cells (PVC), salt-secreting chloride cells,
and pale-staining mucous cells. These chloride cells
contained abundant mitochondria and tubulovesicular
system and are involved in ionic transport with a
potential role in detoxification. The surface of the
secondary lamellae (site of gaseous exchange) consisted
of overlapping or interdigitating PVC supported and
separated by pillar cells. Other cells were found
within the gill epithelium and interstitial connective
tissues, including lymphocytes, macrophages, monocytes,
telocytes, stem cells, astrocytes, and neuroepithelial
cells. The immunohistochemical analysis revealed that
APG-5, iNOS-2, IL-1{\beta}, NF-{\kappa}B, and TGF-B
showed positive immunoreactivity in macrophages. The
epithelium of the primary gill lamellae contained
positive-GFAP astrocytes and S100 protein --- chloride
cells. The stem cells expressed SOX9, myostatin, and
Nrf2. Neuroendocrine cells expressed S100 protein. In
conclusion, the current work suggests that the gills of
molly fish are multifunctional organs and are involved
in immune reactions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shin:2023:SRS,
author = "So Ryung Shin and Hyeon Jin Kim and Jae Won Kim and
Dae-Hyeon Kwon and Junghwa Choi and Jung Jun Park and
Jung Sick Lee",
title = "Sex Ratio, Spawning Period, and Sexual Group Maturity
of the Largehead Hairtail \bioname{Trichiurus
japonicus} ({Teleostei: Trichiuridae}) in
\geoname{Korean} Waters",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "194",
day = "07",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040194",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/194",
abstract = "This study was performed to obtain information on the
sex ratio, spawning period, and size at sexual maturity
for fishery management of the largehead hairtail
Trichiurus japonicus in Korean waters. The overall sex
ratio (female, F; male, M) was 1:0.46 (n = 1274
females:589 males, 68.4\% female) and as fish increased
in length, the proportion of females increased. The
oocyte development pattern was group-synchronous
development, for which oocyte groups at different
stages can be identified within the same ovary. The
average gonadosomatic index (GSI) showed two peaks in
June (3.03) and August (3.10) in females and in May
(1.81) and September (2.24) in males. The median value
of GSI showed two peaks in May (2.65F, 1.78M) and
September (2.55F, 1.87M) for both females and males. As
a result of analyzing the GSI and the monthly gonadal
developmental stages, the main spawning season was
estimated to be bi-annual (May--June and
September--October). The anal length of fish at 50\%
sexual maturity was analyzed using a logistic
regression model and was determined to be 16.38 cm
(female) and 18.31 cm (male).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Alvarez-Fuentes:2023:REC,
author = "Carlos J. Alvarez-Fuentes and Javier Tovar-{\'A}vila
and Jorge Payan-Alejo and Dar{\'\i}o A.
Ch{\'a}vez-Arrenqu{\'\i}n and Isaias H. Salgado-Ugarte
and Felipe Amezcua",
title = "Reproductive Ecology of the \geoname{Chilean} Round
Ray (\bioname{Urotrygon chilensis}, {G{\"u}nther},
1872) in the \geoname{Southern Gulf of California}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "193",
day = "04",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040193",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/193",
abstract = "The Chilean round ray (Urotrygon chilensis,
G{\"u}nther, 1872) is commonly caught as bycatch in the
Mexican Pacific, but changes in its reproductive
ecology as a consequence of fishery effects have never
been investigated. In this work, the reproductive
ecology of this species was determined in the Southern
Gulf of California (SGC). Total length (TL) ranged from
11.4 to 51.6 cm, and females were larger than males.
Size at maturity (TL 50) was estimated at 27.5 cm for
females and 25.3 cm for males. The seasonal variation
of mature individuals, the presence of pregnant
females, the mean oocyte size, the size of embryos and
the smallest free-living specimens along the year
suggest the existence of two reproductive periods
during the year in the SGC, and the size of birth was
estimated to be 11.2 to 15.6 cm TL. The average
fecundity was 2.14 embryos. Spatial variations detected
in size at maturity, fecundity, and reproductive cycle
along the Mexican Pacific coast suggest the presence of
separated populations. Some of these differences could
be related to differences in maximum size attained in
each region, though fishing pressure and environmental
factors could have an important role, too.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Aizonou:2023:ESD,
author = "Romaric A{\"\i}zonou and Ars{\`e}ne Mathieu Houssou
and Guillaume Koussovi and Comlan Eug{\`e}ne Dessouassi
and Mardoch{\'e}e Ephra{\"\i}m Achoh and Sessien
Lionnel Hounhouedo and Clovis Idossou
Hountch{\'e}m{\`e} and Hyppolite Agadjihou{\`e}d{\'e}",
title = "Effect of Stocking Density and Feeding Strategy on
Zootechnical Parameters and Profitability of
\geoname{Nile} Tilapia (\bioname{Oreochromis
niloticus}) Reared in Floating Cages in
\geoname{Toho-Todougba Complex Lagoon in Benin
Republic}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "192",
day = "03",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040192",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/192",
abstract = "This study aimed to compare the growth performance and
economic profitability of rearing Oreochromis niloticus
in floating cages using three feeding strategies (5
days a week, 6 days a week, and 7 days a week) coupled
with three stocking densities (20, 40, and 60
fish{\cdot}m {$-$3}). Male monosex fish measuring 6.44
{\pm} 0.18 cm and weighing 3.42 {\pm} 0.7 g were used.
The controls were weighed every 15 days from the 35th
day of rearing until 185 days to assess zootechnical
parameters. BioMar feed (Efico Cromis 832F) was used
during the study, and the feed ration was adjusted for
each treatment every 2 weeks. At the end of the study,
the average weights varied from 293.09 g to 468.41 g
for the conditions of 40 fish{\cdot}m {$-$3} with a
5/7-day diet and 20 fish{\cdot}m {$-$3} with a 7/7-day
diet, respectively. The yield per m 2 values were
between 7.11 kg and 23.17 kg, respectively. On the
other hand, the economic profitability was better with
the condition of 40 fish.m {$-$3} with daily feeding.
These results suggest that in the Toho-Todougba lagoon
complex, a stocking density between 40 and 60
fish{\cdot}m {$-$3} coupled with daily feeding (three
times a day) promotes the optimization of fish
production and profitability of fish farming.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2023:LFG,
author = "Kai Liu and Zhaoxiang Sun and Chun Yang and Li Jan Lo
and Jun Chen",
title = "Loss-of-Function of \gene{xpc} Sensitizes Zebrafish to
Ultraviolet Irradiation",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "191",
day = "03",
month = apr,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040191",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/191",
abstract = "Xeroderma pigmentosum complementation group C (XPC)
protein recognizes bulky DNA adducts to initiate global
genomic nucleotide excision repair (GG-NER). Humans
carrying germline mutations in the XPC gene display
strong susceptibility to skin and certain internal
cancers. In addition to its role in NER, recent studies
have indicated that XPC is also involved in other DNA
damage repair pathways and transcription regulation. In
this report, we generated a zebrafish xpc knockout
mutant. Zebrafish xpc {$-$}/{$-$} mutant fish develop
relative normally and are fertile. However, the mutant
embryos were more sensitive to ultraviolet (UV)
irradiation. Upon UV irradiation, compared with the
wild type embryos, mutant embryos accumulated
significantly higher levels of unrepaired DNA damages
and apoptotic cells, which led to more severe abnormal
development. Transcriptome analysis showed that the p53
signal pathway and apoptosis were enriched in the over
upregulated genes in UV-irradiated mutant embryos,
suggesting that high levels of unrepaired DNA lesions
activated p53 to trigger apoptotic activity in mutant
embryos. More interestingly, up to 972 genes in the
untreated mutant embryos were differentially expressed,
compared with those in the untreated WT. Among these
differentially expressed genes (DEGs), 379 genes did
not respond to UV irradiation, indicating that Xpc
plays a role in addition of DNA damage repair. Our
results demonstrate that Xpc is an evolutionally
conserved factor in NER repair. Zebrafish xpc
{$-$}/{$-$} mutant also provides a platform to study
other functions of Xpc beyond the DNA damage repair.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rusco:2023:ZBT,
author = "Giusy Rusco and Michele {Di Iorio} and Stefano
Esposito and Pierpaolo Gibertoni and Emanuele Antenucci
and Valentino Palombo and Alessandra Roncarati and
Nicolaia Iaffaldano",
title = "Zootechnical Brown Trout (\bioname{Salmo trutta} {L}.
1758) Ovarian Fluid Fails to Upregulate the Swimming
Performances of Native \geoname{Mediterranean} Brown
Trout (\bioname{Salmo cettii} {Rafinesque}, 1810) Sperm
in the \geoname{Biferno River}",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "190",
day = "31",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040190",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/190",
abstract = "In external fertilizer fish, ovarian fluid (OF) seems
to play a key role in fertilization success, improving
spermatozoa swimming performance. These OF/sperm
interaction mechanisms are frequently species-specific
and/or population-specific and could decrease the risk
of genetic introgression of wild populations from
introduced or escaped zootechnical individuals. The
Mediterranean brown trout (Salmo cettii) is threatened
by genetic introgression with strains of domestic brown
trout (Salmo trutta) that were introduced for
recreational purposes. The aim of our study was to test
if native S. cettii females, rather than zootechnical
S. trutta, produce OF with a greater ability to
upregulate the sperm motility of conspecific males.
Thus, we compared the sperm swimming performances of
males inhabiting the Biferno River (Molise region ---
Southern Italy) activated in native S. cettii vs.
zootechnical S. trutta female's OFs. In our study,
native females' OFs (20\% diluted), compared to spring
water, has the ability to significantly boost the sperm
performance of the autochthonous males, while
zootechnical S. trutta fails. These preliminary results
suggest that OF-sperm interactions could potentially
influence or direct the hybridization mechanisms
involving the native Mediterranean trout inhabiting the
Biferno River and the domestic lineage of brown trout
introduced in the past.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:JTF,
author = "Chao-Ching Chen and Hsin-Zong Lin and Kuo-Wei Lan",
title = "{Juvenile and Trash Fish Excluder Device (JTED)} for
\geoname{Taiwanese} Bottom Trawl",
journal = j-FISHES,
volume = "8",
number = "4",
pages = "189",
day = "30",
month = mar,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8040189",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:41 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/4/189",
abstract = "Juvenile and trash fish excluder devices (JTEDs) are
used to mitigate the ecological impact of trawling.
Trawling has a large ecological footprint in Taiwan.
Thus, this study designed three JTEDs, which differed
only in grid interval, for single boat bottom otter
trawls that are commonly used in Taiwan. The designed
JTEDs were tested in the waters off of Kaohsiung on a
Kaohsiung-based bottom trawler, and the results were as
follows. (1) At least 300 species were caught, and 189
of these species had little commercial value; their
weight accounted for 52.5\% of the total catch. (2) The
three JTEDs had good exclusion performance. (3) The
ratios of the weight of bycatch excluded to the weight
of total catch were 7.9\%, 29.1\%, and 36.2\%, for grid
intervals of 10, 15, and 20 mm, respectively. (4) JTEDs
with grid intervals of 10, 15, and 20 mm were
associated with a 0.4\%, 11.5\%, and 19.0\% loss in
revenue, respectively. (5) Mature (and thus
commercially valuable) Loliginidae tended to be
excluded when the grid interval was 20 or (especially)
15 mm. In general, the designed JTEDs should be used at
a grid size of 15 mm because this strikes the best
balance between profitability and sustainability.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fraser:2023:EGG,
author = "Thomas W. K. Fraser and Tom J. Hansen and Per Gunnar
Fjelldal",
title = "Environmental and Genetic (\gene{vgll3}) Effects on
the Prevalence of Male Maturation Phenotypes in
Domesticated \geoname{Atlantic} Salmon",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "275",
day = "22",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050275",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/275",
abstract = "Pre-harvest male maturation is problematic for
Atlantic salmon (Salmo salar) farmers and is regulated
by the environment and genetics (e.g., vgll3). Five
families of all-male salmon parr (produced using YY
males crossed with XX females) with different vgll3
genotypes were split between three environmental
regimes in January 2018. The ``advanced maturation''
regime used elevated temperature (16 {$^\circ $}C) and
continuous light from January 2018 with post-smolt
maturation assessed in March 2018. The ``extended
freshwater'' regime used ambient freshwater (1--16
{$^\circ $}C) and simulated natural photoperiod (SNP)
with post-smolt maturation assessed in November 2018.
The ``sea transfer'' regime used ambient temperatures
(1--14 {$^\circ $}C) and SNP in freshwater until May
2018 when they were transferred to 9 {$^\circ $}C
seawater with natural photoperiod for 2.5 years (final
mean weight of circa. 14 kg) and assessed for
post-smolt maturation, 1 sea-winter (1 SW) maturation,
and 2 sea-winter (2 SW) maturation in the autumn
(November/December) of 2018, 2019, and 2020,
respectively. Post-smolt maturation was highest in the
advanced maturation and extended freshwater regimes
(39--99\% depending on family) and lowest in the sea
transfer regime (0--95\% depending on family). In the
sea transfer regime, maturity incidence increased over
time (0--95\% post-smolt maturation, 1--100\% 1 SW, and
50--90\% 2 SW maturation, depending on family). In all
regimes, those homozygous for the pre-designated vgll3
``early'' maturing allele had the highest incidences of
maturation whilst those homozygous for the ``late''
allele had the lowest. A low percentage of 2 SW
phenotypic and genetic females were found (0--5\%
depending on family), one of which was successfully
crossed with an XY male resulting in progeny with an
approx. 50/50 sex ratio. These results show (i)
post-smolt maturation varies dramatically depending on
environment although genetic regulation by vgll3 was as
expected, and (ii) crossing YY sperm with XX eggs can
result in XX progeny which can themselves produce
viable progeny with an equal sex ratio when crossed
with an XY male.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Artero:2023:CWF,
author = "C{\'e}line Artero and Christopher C. Koenig and
Jessica E. Marsh",
title = "Are the Coastal Waters of \geoname{French Guiana} a
Source or Sink Habitat for \geoname{Atlantic} Goliath
Grouper \bioname{Epinephelus itajara}?",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "274",
day = "22",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050274",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/274",
abstract = "The coastal waters of French Guiana are amongst the
most turbid and nutrient rich in the world, and as
such, they favour the settlement and early development
of several species of marine fish. Recent work has
revealed a high abundance of Atlantic Goliath Grouper
(Epinephelus itajara) in these waters, yet little is
known about this species' reproductive biology, which
this study aims to complement. During the period from
2010 to 2013, we surveyed Goliath Grouper at three
rocky sites along the French Guiana coast and examined
602 individuals for evidence of maturation. Satellite
tags were deployed on five individuals and an acoustic
survey was conducted to identify potential spawning
movements and activity. We found no evidence of
sexually active individuals or local spawning
aggregations. Most individuals showed high site
fidelity, but one fish was located 1150 km away, near
Trinidad and Tobago. We discuss these findings in the
context of the wider literature and postulate that
Goliath Grouper inhabiting French Guiana reefs
originate from spawning sites in northern Brazil and
disperse to westerly reefs with the onset of maturity.
We propose that the Goliath Grouper populations of
South America and Caribbean are linked, and that
management of Brazilian source populations could have
wide-reaching impacts on Goliath Grouper stocks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Smit:2023:MPM,
author = "Willem J. Smit and Maarten P. M. Vanhove and
Ngonidzashe A. G. Moyo and Wilmien J. Luus-Powell",
title = "Metazoan Parasites of \geoname{Mozambique} tilapia
(\bioname{Oreochromis mossambicus}) Native to
\geoname{Lake Urema, Mozambique}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "273",
day = "20",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050273",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/273",
abstract = "Mozambique tilapia Oreochromis mossambicus (Peters,
1852), an important aquaculture species investigated
herein, is at risk and listed as vulnerable on the IUCN
Red List. During a preliminary survey conducted in
2011, 30 Mozambique tilapia from Lake Urema in
Gorongosa National Park in central Mozambique, were
examined for metazoan parasites. Two groups of
ectoparasites were recovered consisting of two species
of copepods and a monogenean. Endoparasites retrieved
were in the larval stage and included one trematode and
one nematode species. This study provides new parasite
records for O. mossambicus in Mozambique and forms a
baseline for monitoring against the presence of
ichthyoparasites associated with the introduction in
neighbouring systems of non-native fish such as Nile
tilapia Oreochromis niloticus (Linnaeus, 1758), a fish
of worldwide importance in capture fisheries and
especially aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bolognini:2023:COD,
author = "Luca Bolognini and Cristina Frittelloni and Francesca
Perretta and Martina Scanu and Fabio Grati",
title = "From Crisis to Opportunity: Developing a Virtual
Marketplace to Enhance Sustainability and Resilience in
Small-Scale Fisheries",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "272",
day = "19",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050272",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/272",
abstract = "In a context in which climate change,
overexploitation, and environmental degradation are
continuously progressing, the sustainable use of the
sea is a key target, both for resources and fishery
operators. With the aim of enhancing the sustainability
and competitiveness of the entire fishery sector, an
innovation brokering event was organized. Via the
application of a participatory, interactive, and
bottom-up approach, different actors in the Italian SSF
sector were stimulated to work together to find
innovative solutions to day-by-day problems. During the
consultation, and between the identified problems,
emerged the lack of cooperation for marketing activity
and effective strategies for seafood product
development. A pilot action consisting in co-designing
a virtual marketplace (VirMa) was the result of the
event, intending to facilitate the direct sale of
seafood products, explore this new and valuable market,
and establish a direct line with consumers. The VirMa
application was developed as a value chain network,
trying to add value to seafood products. However, an
information and communication tool, such as a VirMa,
could contribute to increasing the resilience of the
SSF sector to market constraints even during unforeseen
events, such as a pandemic.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Seixas:2023:DTS,
author = "Manuel J. Seixas and Rodrigo R. Domingues and
Agostinho Antunes",
title = "Decoding the Transcriptome of Sharks, Rays, and
Chimaeras: Insights into Their Physiology, Morphology,
Evolution, and Biomedical Applications",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "271",
day = "19",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050271",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/271",
abstract = "Chondrichthyes (including sharks, rays, and chimaeras)
are a class of jawed cartilaginous fishes (with
skeletons composed primarily of cartilage), with major
relevance to the marine ecosystems and to humanity.
However, cartilaginous fishes are facing various
threatens, inflicting abrupt declines in their
populations. Thus, critical assessment of available
molecular genetic variation, particularly retrieved
from Chondrichthyans' transcriptomic analyses,
represents a major resource to foster genomics research
in this ancient group of vertebrate species. Briefly,
RNA-Seq involves the sequencing of RNA strands present
on a target tissue, which can assist genome annotation
and elucidate genetic features on species without a
sequenced genome. The resulting information can unravel
responses of an individual to environmental changes,
evolutionary processes, and support the development of
biomarkers. We scrutinized more than 800 RNA-Seq
entries publicly available, and reviewed more than one
decade of available transcriptomic knowledge in
chondrichthyans. We conclude that chondrichthyans'
transcriptomics is a subject in early development,
since not all the potential of this technology has been
fully explored, namely their use to prospectively
preserve these endangered species. Yet, the
transcriptomic database provided findings on the
vertebrates' evolution, chondrichthyans' physiology,
morphology, and their biomedical potential, a trend
likely to expand further in the future.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Campos-Sanchez:2023:ECF,
author = "Jose Carlos Campos-S{\'a}nchez and Francisco A.
Guardiola and Mar{\'\i}a {\'A}ngeles Esteban",
title = "Effects of Cantharidin on Fish Erythrocytes, Tumor
Cell Lines, and Marine Pathogenic Bacteria",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "270",
day = "19",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050270",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/270",
abstract = "Cantharidin, a toxic monoterpene secreted by blister
beetles, has long been used in traditional Chinese and
modern medicine for its unique properties. However,
despite its widespread use, its effects on fish have
not been studied. The aim of this study was to evaluate
the potential therapeutic applications of cantharidin
in fish by examining its antioxidant, hemagglutinating,
hemolytic, and cytotoxic activities at different
concentrations (0, 0.625, 1.25, 2.5, 5, and 10 {\mu}g
mL {$-$1}) in three different cell lines. In addition,
the study explored the bactericidal and bacteriostatic
properties of cantharidin against various fish
pathogenic bacteria. The results revealed that there
were no significant differences in antioxidant,
hemagglutinating, or hemolytic activities between the
different concentrations of cantharidin tested.
However, the study found that cantharidin exhibited
dose- and time-dependent cytotoxicity in seabream
(Sparus aurata) erythrocytes and in SAF-1, PLHC-1, and
Hela cell lines, resulting in morphological changes
indicative of apoptosis. Interestingly, the highest
dose of cantharidin tested demonstrated potent
bactericidal activity against four marine fish
opportunistic bacteria, including Vibrio harveyi, V.
anguillarum, Photobacterium damselae, and Tenacibaculum
maritimum, but no statistically significant changes in
bacteriostatic activity were observed against any of
the bacteria tested. Overall, these results provide
valuable information on the potential therapeutic
applications of cantharidin in fish aquaculture.
Further research is needed to fully understand the
mechanisms of action and to explore possible preventive
uses of cantharidin in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:WLR,
author = "Yushan Li and Mingjun Feng and Liangliang Huang and
Peiyu Zhang and Hongxia Wang and Jingwei Zhang and
Yuehan Tian and Jun Xu",
title = "Weight--Length Relationship Analysis Revealing the
Impacts of Multiple Factors on Body Shape of Fish in
\geoname{China}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "269",
day = "19",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050269",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/269",
abstract = "The weight--length relationship (WLR) of fish is a
crucial tool in fish biology research and has received
extensive attention. However, fish growth is influenced
by many factors, and the WLR also changes accordingly.
Our aim was to investigate how fish body shape is
affected by various factors by analyzing the existing
parameters of the relationship between fish body length
and weight. We analyzed 198,354 fish specimens
belonging to 402 species of 82 families in China and
investigated the change of fish body shape in the
function of their ecology. Herbivorous fish tended to
be shorter and fatter than carnivorous fish, and
omnivorous fish fall somewhere in between. This
difference could be due to variations in feeding habits
and the availability of food sources. Additionally,
fish living in lentic waters tended to have a shorter
and fatter body shape compared to those living in lotic
waters. This could be attributed to differences in
swimming behavior in these environments. Furthermore,
our results showed that the b value decreased as
altitude increased, and fish tended to be thinner and
longer due to lower oxygen and temperature levels in
high-altitude waters. Overall, our study provides
valuable insights into the WLR of fish and the impact
of multiple factors on fish body shape.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dambrosio:2023:SLQ,
author = "Angela Dambrosio and Nicoletta Cristiana Quaglia and
Maria Antonietta Colonna and Flavia Capuozzo and
Francesco Giannico and Simona Tarricone and Anna Caputi
Jambrenghi and Marco Ragni",
title = "Shelf-Life and Quality of Anchovies
(\bioname{Engraulis encrasicolus}) Refrigerated Using
Different Packaging Materials",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "268",
day = "18",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050268",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/268",
abstract = "The identification of sustainable materials as an
alternative to plastic is fundamental for the
protection of the environment and for the safeguard of
marine ecosystems. The replacement of plastic with
organic materials for fish storage might affect its
shelf-life due to the high concentration of oxidizable
fatty acids in fish flesh. This study investigated the
effectiveness of two organic packaging materials,
namely coconut fiber (Coc) and paper (Pap), as
alternatives to the conventionally used polystyrene
(Pol), on the shelf-life, hygienic parameters, and
quality traits of anchovies refrigerated for one (T0),
three (T1), or seven (T2) days. The hygienic quality of
anchovies packaged in the organic Pap and Coc boxes was
better than the traditional Pol, probably due to the
higher porosity of the packaging, which allows a higher
bacterial proliferation. Results of the T-BARS test
showed a lower (p {$<$} 0.05) malondialdehyde
concentration in fillets from the Pap group, which
increased (p {$<$} 0.05) from the first to the seventh
day of storage. The polyunsaturated fatty acids
concentration did not differ between groups, but it
decreased (p {$<$} 0.05) from T0 to T2. Coconut fiber
and paper are worthy of further investigation since
these packaging materials did not affect the shelf-life
and fatty acid profile of anchovies during storage.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2023:GRM,
author = "Chien-Yu Wu and Tsung-Han Lee and Deng-Yu Tseng",
title = "Glucocorticoid Receptor Mediates Cortisol Regulation
of Glycogen Metabolism in Gills of the Euryhaline
Tilapia (\bioname{Oreochromis mossambicus})",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "267",
day = "18",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050267",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/267",
abstract = "In this study, we investigated the effects of cortisol
on the regulation of the glycogen metabolism biomarkers
glycogen synthase (GS) and glycogen phosphorylase (GP)
in the glycogen-rich cells of the gills of tilapia
(Oreochromis mossambicus). In the gills of tilapia, GP,
GS, and glycogen were immunocytochemically colocalized
in a specific group of glycogen-rich cells adjacent to
the gills' main ionocytes and mitochondria-rich cells.
Cortisol plays a vital role in the regulation of
physiological functions in animals, including energy
metabolism, respiration, immune response, and ion
regulation. However, no studies have elucidated the
mechanisms regulating cortisol and glycogen-rich cells
in the gills. Therefore, we treated tilapia larvae with
exogenous cortisol and a glucocorticoid receptor (GR)
antagonist to investigate the regulatory mechanisms
between cortisol and glycogen-rich cells in the gills.
Our results showed that cortisol promoted the
expression of gill glycogen phosphorylase isoform
(GPGG) mRNA via GR, whereas the GS gene expression
remained unaffected. We also found that GR mRNA was
colocalized with some glycogen-rich cells in the gills,
further confirming our hypothesis that cortisol
directly acts on glycogen-rich cells in the gills of
tilapia and regulates glycogen metabolism by promoting
GPGG mRNA expression.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hill:2023:NHU,
author = "Jeffrey E. Hill and Allison Durland Donahou and Emily
S. Wooley and Lauren N. Lapham and Quenton M. Tuckett",
title = "Narrowing the Horizon: Using Known Invasives and
Propagule Pressure to Focus Risk Screening Efforts on
Potential Invasives",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "266",
day = "17",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050266",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/266",
abstract = "About 11 million marine ornamental fish of ca 1800
non-native species are imported into the USA each year.
Selecting species for risk assessment is daunting for
such a diverse pathway. Herein, we discuss a focused
method for species selection: choosing important taxa
related to known invaders in high-volume pathways and
narrowing prospective species lists to manageable
groups of potential hazards. We provide an example
using 11 damselfishes, a family with high volume in
trade and one of the species established in USA waters.
We used a specialized literature review and a risk
screening tool (Aquatic Species Invasiveness Screening
Kit) to provide an estimate of risk of invasiveness of
marine waters of Florida. The established species was
identified as a hazard and potential invasive. All
other species scored well below the threshold for
invasiveness and future climate had little effect on
estimates of invasiveness. The analysis revealed little
need for additional risk assessment or prohibitions on
damselfishes in trade. Education, monitoring, and early
detection and rapid response were the main risk
management recommendations. The focused species
selection process employed herein provided hazard
identification and preliminary risk estimates for just
11 species, but collectively, they represent 40\% by
volume of fishes imported in the marine ornamental
trade.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Danilov:2023:SOP,
author = "Diana Danilov and Lorena Dediu and Nicoleta Alexandra
Damir and Valentina Coatu and Luminita Lazar",
title = "Screening for Organic Pollutants in the \geoname{Black
Sea} Turbot (\bioname{Scophthalmus maeoticus})",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "265",
day = "17",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050265",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/265",
abstract = "The health of aquatic organisms can be affected due to
anthropogenic activities and limited actions to reduce
the pollution of the Black Sea. The accumulation of
organic pollutants (OPs) in the aquatic environment
occurs in water, sediment, and then biota. The turbot
(Scophthalmus maeoticus) is a benthic fish of
commercial interest scarcely studied in the Black Sea
region, and none of the studies researched OP
concentrations in its main tissues. In this paper,
polycyclic aromatic hydrocarbons (PAHs) and POPs,
organochlorine pesticides (OCPs) and polychlorinated
biphenyls (PCBs) were determined in water, sediment,
and turbot muscles, gills, gonads, and livers, to
research their accumulation level. The determinations
were made with gas chromatography on turbots sampled in
2021 from the Romanian Black Sea waters. OCPs ---
p,p'DDT and its metabolites p,p'DDE, p,p'DDD --- are
dominant in the turbot tissues. From PAHs, benzo(g,h,
i)perylene was the dominant compound, while for PCBs it
was PCB 52. The OPs' presence in the wild turbot is due
to river input, dredging and coastal rehabilitation
works, industrial activities and contaminated food and
poses a risk to human health due to the exceeding
maximum allowable concentration for human consumption
in Romania and the European Union.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cheng:2023:MBS,
author = "Ann-Chang Cheng and Rolissa Ballantyne and Shieh-Tsung
Chiu and Chun-Hung Liu",
title = "Microencapsulation of \bioname{Bacillus subtilis}
{E20} Probiotic, a Promising Approach for the
Enrichment of Intestinal Microbiome in White Shrimp,
\bioname{Penaeus vannamei}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "264",
day = "16",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050264",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/264",
abstract = "Microencapsulation is an advanced technique used to
improve the viability of probiotics and minimize
sensitivity during processing, storage, and in the
gastrointestinal environment. Two dietary treatments
including a control and an encapsulated probiotic,
Bacillus subtilis E20 (EP), were used to evaluate the
efficacy in improving the intestinal microbiome of
white shrimp, Penaeus vannamei, after a 60-feeding
trial. The 16S rDNA next-generation sequencing (NGS)
analysis indicated that shrimp fed the EP diet
generated higher amplicon reads than shrimp fed the
control diet. No significant differences were observed
in the \alpha -diversity index of the intestinal
microbiota of shrimp that were fed the control and EP
diet. At the phylum level, Proteobacteria was
relatively abundant in the microbiota of shrimp fed
both the control and EP diet. The treatment with EP
increased the expression of Tenericutes, Bacteroidetes,
and Firmicutes, more than the control. The PC analysis
revealed that the EP diet altered the bacterial profile
in shrimp's intestines into forming different clusters.
Unique genera such as Luteolibacter, Simkaniaceae,
Haemophilus, Pirellulaceae, Filomicrobium,
Sphingomonas, and Erysipelotrichaceae UCG-003 along
with well-known probiotic genera Bacillus and
Lactobacillus were found in the intestine of shrimp fed
the EP diet. The PCA eigenvector plots indicated a
higher abundance of Bacillus in shrimp fed with EP
diet, but a higher abundance of Vibrio in shrimp fed
with control diet. These results suggest that
encapsulated B. subtilis E20 can be beneficial to
shrimp microbiota.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Choi:2023:CCA,
author = "Young Jae Choi and Seul Gi Na Ra Park and A-Hyun Jo
and Jun-Hwan Kim",
title = "Correction: {Choi et al. Physiological Effect of
Extended Photoperiod and Green Wavelength on the
Pituitary Hormone, Sex Hormone and Stress Response in
Chub Mackerel, \bioname{Scomber japonicus}. Fishes
2023, {\bf 8}, 77}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "263",
day = "16",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050263",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Choi:2023:PEE}.",
URL = "https://www.mdpi.com/2410-3888/8/5/263",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Feng:2023:LMB,
author = "Liu-Ying Feng and Li-Ping Yan and Run-Wei Li and
Sheng-Fa Li and Jia-Hua Cheng and Yan Jin",
title = "{LC-MS} Based Metabolomic Profiling of Largehead
Hairtail (\bioname{Trichiurus japonicus}) Ovary Reveals
Metabolic Signatures of Ovarian Developmental Process
({II--IV})",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "262",
day = "14",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050262",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/262",
abstract = "Trichiurus japonicus is an economically important fish
that ranks 11th in global marine fish capture
production. However, the reproductive characteristics
of this fish have undergone notable changes in recent
decades, potentially affecting the quality of offspring
and sustainable utilization. To improve our
understanding of the physiological regulation of
maturation in T. japonicus, untargeted liquid
chromatography mass spectrometry was utilized to
identify the small molecules that characterize the
comprehensive metabolic profiles of ovaries during
ovary development from stage II to stage IV. According
to the results of OPLS-DA, the ovarian metabolite
profiles of the three developmental stages were
separated. The concentrations of 124 and 100
metabolites were significantly altered between stage II
vs. III and III vs. IV, respectively. Lipids and
lipid-like molecules accounted for the largest
proportion of the altered metabolites, followed by
amino acids, peptides, and analogues. The significantly
altered metabolites-enriched pathways differed slightly
between stages II and III and stages III and IV.
Steroid-related pathways were heavily affected during
stages II to III, while significantly altered
metabolites from stages III to IV were involved in
oocyte-maturation-related pathways. Through
metabonomics analysis, potentially important metabolic
pathways and metabolites between different ovarian
stages were detected, providing basic information for
further investigation of maturation mechanisms in wild
fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Osorio:2023:PCB,
author = "Brendon James Osorio and Grzegorz Skrzypek and Mark
Meekan",
title = "Parasitic Copepods as Biochemical Tracers of Foraging
Patterns and Dietary Shifts in Whale Sharks
(\bioname{Rhincodon typus} {Smith}, 1828)",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "261",
day = "14",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050261",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/261",
abstract = "Understanding the diet of whale sharks (Rhincodon
typus) is essential for the development of appropriate
conservation strategies for the species. This study
evaluated the use of the parasitic copepod (Pandarus
rhincodonicus) as a proxy to infer short-term foraging
habitats and trophic positions of whale shark hosts. To
accomplish this, bulk stable carbon ({\delta} 13 C) and
nitrogen ({\delta} 15 N) isotope compositions were
analysed from 72 paired samples of whale shark skin
(dermal) tissues and copepods collected across six
years at the Ningaloo Reef aggregation site, Western
Australia. This study found that {\delta} 15 N from
parasites and whale shark hosts were strongly
correlated. As turn-over times of the parasite and
whale shark differ (months vs. years, respectively),
the ability of copepods to predict {\delta} 15 N values
indicates that the trophic positions of whale sharks
remain consistent across these timeframes.
Contrastingly, {\delta} 13 C in the parasite and host
were weakly correlated, likely reflecting differences
in the physiology and lifecycle of the copepod parasite
compared to the host. Our results suggest {\delta} 15 N
from parasitic copepods provides a reliable proxy of
the trophic position of their whale shark hosts, but
interpretation of {\delta} 13 C values as a proxy for
the host will require future studies on the lifecycle
of P. rhincodonicus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Easton:2023:CSO,
author = "Blair Alexander Andrew Easton and Andrew Boon and Joe
Richards and Kevin Scott",
title = "Comparing the Size at Onset of Sexual Maturity of
Edible Crab (\bioname{Cancer pagurus}, {Cancridae}) in
\geoname{Berwickshire} and \geoname{Northumberland}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "260",
day = "13",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050260",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/260",
abstract = "The literature suggests regional variations in the
size at which sexual maturity is reached for
commercially important edible crab (Cancer pagurus),
worth GBP 74.3 million annually, which could have
implications for regional fisheries management.
Berwickshire and Northumberland are geographically
divided by the Scotland and England border and remain
within the Berwickshire and North Northumberland Coast
SAC (Special Area of Conservation). Each are managed by
differing fisheries authorities and Minimum
Conservation Reference Sizes (MCRS). Morphometric
measurements were recorded for each C. pagurus
individual to categorise morphometric maturity using
segmented regression, with gonadal maturity categorised
using visual gonad characteristics and general linear
model regressions to compare onset in sexual maturity.
Results showed regional variations for gonadal maturity
with males reaching sexual onset at a carapace width
size of 108.5 mm in Berwickshire and 109.9 mm in
Northumberland; females at a size of 126.8 mm in
Berwickshire and 120.8 mm in Northumberland. This was
also true for morphometric maturity based on chelae
height, that males (141.1 mm) and females (134.7 mm)
from Berwickshire were morphometrically mature at
greater sizes than males (130.1 mm) and females (120.8
mm) from Northumberland. This study shows that the
respective MCRS in both regions are appropriate for the
C. pagurus populations, but implications for fisheries
management could be present.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ahmed:2023:CSG,
author = "Shaimaa A. A. Ahmed and Abdelhakeem El-Murr and Yasser
Abd Elhakim and Mohamed M. Metwally and Amany Abd El
Aziz Gharib and Shimaa A. Amer and Elsayed M. Younis
and Abdel-Wahab A. Abdel-Warith and Simon J. Davies and
Enas N. M. Khalil",
title = "Comparative Study on Ginger Powder and Ginger Extract
Nanoparticles: Effects on Growth, Immune--Antioxidant
Status, Tissue Histoarchitecture, and Resistance to
\bioname{Aeromonas hydrophila} and \bioname{Pseudomonas
putida} Infection in \bioname{Oreochromis niloticus}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "259",
day = "13",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050259",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/259",
abstract = "A 10 week feeding trial was conducted to evaluate the
potential effects of ginger powder (GP) and ginger
extract nanoparticles (GNPs) on the growth parameters,
digestive enzymes (lipase and amylase) activities,
blood hematology, blood biochemical indices, immune
indices (interleukin 10, immunoglobulin M, nitric
oxide, and lysozymes), antioxidant activity,
histological characteristics of kidney, spleen, liver,
and intestine, and resistance to Aeromonas hydrophila
or Pseudomonas putida infection in Nile tilapia,
Oreochromis niloticus. Fish (n = 225, 27.01 {\pm} 0.15
g) were stocked in 15 glass tanks (50 $ \times $ 40 $
\times $ 60 cm) and randomly allocated to five
experimental treatments (TRTs) in triplicate (15
fish/replicate, 45 fish/TRT), consisting of five
isocaloric--isonitrogenous diets. The treatments
comprised the basal diet (1) without any additives
(control group, CON), (2) with 0.5\% GP (GP0.5), (3)
with 1\% GP (GP1), (4) with 0.5\% GNPs (GNPs0.5), and
(5) with 1\% GNPs (GNPs1). Fish were manually fed to
satiety three times a day (at 9 a.m., 12 p.m., and 2
p.m.). Fish were weighed at the start of the
experiment, then the body weight, weight gain, feed
intake, and feed conversion ratio were determined at
the end of the experiment. At the end of the feeding
period, 15 fish/TRT were intraperitoneally inoculated
with two pathogenic bacterial strains (A. hydrophila or
P. putida) in two separate challenge tests. Blood
samples were collected from each TRT at two aliquots
for hematological and biochemical analysis at the end
of the feeding period. A significant improvement in
fish growth was observed in GP and GNPs TRTs compared
to the control group. There were no significant changes
in the total amount of feed intake/fish in response to
the experimental diets. Diets enriched with GNPs,
particularly the GNPs1 TRT, resulted in a significant
increase (p {$<$} 0.05) in digestive enzyme activity
(lipase and amylase), serum growth hormone level,
proteinogram, and immune indices (lysozyme,
immunoglobulin M, interleukin 10, and nitric oxide). In
addition, a significant increase in hepatic antioxidant
enzymes (superoxide dismutase, reduced glutathione, and
catalase) was observed in fish fed with GNPs-enriched
diets. Survival percentages following bacterial
challenge were higher in GNPs1, followed by GP1 and
GNPs0.5 TRTs. Normal histomorphology was found in
liver, kidney, and spleen tissues in all experimental
TRTs. We conclude that GP and GNPs could be included in
Nile tilapia diets for promoting fish growth, immunity,
antioxidant status, and disease resistance without
harming organ functions. In particular, the most
effective treatment was GNPs1.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Przybyla-Kelly:2023:CPK,
author = "Kasia J. Przybyla-Kelly and Ashley M. Spoljaric and
Meredith B. Nevers",
title = "Correction: {Przybyla-Kelly et al. Round Goby
Detection in \geoname{Lakes Huron and Michigan} --- an
Evaluation of eDNA and Fish Catches. Fishes 2023, {\bf
8}, 41}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "258",
day = "12",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050258",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Przybyla-Kelly:2023:RGD}.",
URL = "https://www.mdpi.com/2410-3888/8/5/258",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2023:CKD,
author = "Dong In Kim",
title = "Correction: {Kim, D. I. A Study on the Metabolic Rate
Change Pattern in F$_2$ Hybrid Sturgeon, the Bester
(\bioname{Huso huso} $ \times $ \bioname{Acipenser
ruthenus}), during the Early Developmental Stage.
Fishes 2023, {\bf 8}, 113}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "257",
day = "12",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050257",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Kim:2023:CKD}.",
URL = "https://www.mdpi.com/2410-3888/8/5/257",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shuai:2023:TPO,
author = "Fangmin Shuai and Jie Li and Shunchao Yu and Jian
Yang",
title = "Temporal Pattern of the Occurrence of
\geoname{Japanese} Glass Eels (\bioname{Anguilla
japonica}) in the \geoname{Pearl River Estuary}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "256",
day = "11",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050256",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/256",
abstract = "Japanese eels (Anguilla japonica) are a typical
migratory fish species with high commercial importance.
The Pearl River estuary in southern China is an
important natural growing ground for Japanese glass
eels, but limited information on Japanese glass eel
population characteristics is available, despite their
ecological importance. In this paper, we examined the
annual patterns of the occurrence of Japanese glass
eels in the Pearl River estuary from 2011 to 2022. The
most frequently occurring Japanese glass eel's total
length is 5.3 cm. The collecting period extended from
December to February, and the collection
catch-per-unit-effort (CPUE) decreased significantly
from 2011 to 2022. The generalized linear model (GLM)
indicated that daily changes in Japanese glass eel
collection were significantly affected by tidal range,
water temperature, and lunar distance. The catch peak
appeared when the tidal range rose to 1.7 m, and the
water temperature dropped below 8 {$^\circ $}C on the
full moon days. Overall CPUE analysis showed no
significant periodic and inter-annual variability in
the period 2011--2022, with the ARIMA model suggesting
that the CPUE is expected to remain stable but low in
the coming years (2023--2026), although recruitment
ultimately depends on the overall spawning stock.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sateriale:2023:BPB,
author = "Daniela Sateriale and Serena Facchiano and Katrin
Kaldre and Giuseppina Forgione and Giuseppa Anna {De
Cristofaro} and Caterina Pagliarulo and Marina
Paolucci",
title = "Benefits of Polyphenol-Based Synbiotics in Crustacean
Diet",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "255",
day = "10",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050255",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/255",
abstract = "Here, the olive leaf extract (OLE) rich in polyphenols
was employed as a prebiotic agent, together with
Lactobacillus reuteri and Bacillus clausii, to develop
synbiotics. The prebiotic effect of olive leaf extract
on the probiotic strains was tested at concentrations
of 0, 50, 100, 400, and 1000 {\mu}g mL {$-$1}, and also
20 and 40 mg mL {$-$1}. Olive leaf extract at 40 mg mL
{$-$1} showed the best prebiotic activity on L. reuteri
and B. clausii. A basal diet and two experimental
synbiotic-containing diets were prepared. The synbiotic
diets were manufactured by adding to the basal diet 5 $
\times $ 10 6 CFU g {$-$1} L. reuteri + 5 $ \times $ 10
6 CFU g {$-$1} B. clausii + 0.25 mg g {$-$1} OLE and 1
$ \times $ 10 7 CFU g {$-$1} L. reuteri + 1 $ \times $
10 7 CFU g {$-$1} B. clausii + 0.25 mg g {$-$1} OLE.
The diets were administered to the freshwater crayfish
Astacus astacus (1.35 {\pm} 0.04 g) in an 84-day
feeding trial. The diet containing 5 $ \times $ 10 6
CFU g {$-$1} L. reuteri + 5 $ \times $ 10 6 CFU g
{$-$1} B. clausii + 0.25 mg g {$-$1} OLE significantly
improved (p {$<$} 0.05) final weight, specific growth
rate, body condition, and survival rate. A significant
growth of Enterobacteriaceae, which include strains
with proven beneficial activities for intestinal health
and general animal welfare, significantly increased in
crayfish fed with synbiotics. The obtained results
could be suitable for functional feed development in
crayfish farming.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tringali:2023:RSD,
author = "Michael D. Tringali",
title = "Reproductive Success Dynamics Could Limit Precision in
Close-Kin Mark--Recapture Abundance Estimation for
\geoname{Atlantic} Goliath Grouper
(\bioname{Epinephelus itajara})",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "254",
day = "10",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050254",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/254",
abstract = "Extra-Poisson variance in annual reproductive success
can affect the precision of abundance estimates made
using `close-kin' mark--recapture procedures. However,
empirical evaluation of that variance in natural marine
populations can be daunting. Here, a scaling method was
used to extend a discrete-time, age-structured model,
facilitating investigation of annual and lifetime
reproductive success dynamics in Epinephelus itajara.
Life tables were synthesized from fishery assessment
vital rates and life history measures. For model
scaling, a precise empirical estimate of effective
population size for the studied population was utilized
as the independent variable, and model sensitivity to
an informative range of adult abundance was evaluated.
The potential for sex reversal to impact reproductive
success dynamics was also investigated, albeit in the
absence of selective fishing pressure and potential
compensatory or depensatory responses. Close-kin
relationships in a genetic sample of $ \approx $300
adults collected from spawning sites in the Florida
Atlantic included numerous full-sibling pairs and
multi-sibling families, which is unusual for
long-lived, iteroparous marine populations with broadly
dispersed larvae. The highly overdispersed reproductive
success dynamics modeled for this population and its
atypical kinship distribution could have ramifications
for planned close-kin mark--recapture analyses. The low
observed effective size also has conservation
implications. Both issues warrant continued genetic
monitoring.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2023:GDP,
author = "Lin Zhou and Jiancao Gao and Yanping Yang and Zhijuan
Nie and Kai Liu and Gangchun Xu",
title = "Genetic Diversity and Population Structure Analysis of
\geoname{Chinese} Mitten Crab (\bioname{Eriocheir
sinensis}) in the \geoname{Yangtze and Liaohe Rivers}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "253",
day = "10",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050253",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/253",
abstract = "Recently, the economic traits of Chinese mitten crab
(Eriocheir sinensis) varieties have had a negative
tendency. Meanwhile, the status of wild germplasm
resources of E. sinensis is unknown, hindering the
utilization of wild germplasm resources and the green
development of the E. sinensis industry. Thus, the
conservation of the wild E. sinensis germplasm resource
is of great significance. To this end, we collected
wild E. sinensis from two different river basins, the
Yangtze River basin, and the Liaohe River basin, and
analyzed the genetic diversity as well as the genetic
differentiation in E. sinensis populations. Based on
eight microsatellite markers, we found moderate genetic
diversity in E. sinensis populations regardless of
river basin. Based on the mitochondrial D-loop region,
we found that all populations are at mutation drift
equilibrium, while the Nm between any two populations
is greater than 1. We hypothesized the existence of
island model gene flow patterns among E. sinensis.
Interestingly, genetic differentiation among E.
sinensis populations was low, except that between
Liaohe and Anqing or Shanghai populations.
Additionally, geometric morphological analysis could
distinguish E. sinensis from different basins, with an
accuracy of 94.2--100\%. Given the similar genetic
diversity in the two basins, the genetic convergence of
E. sinensis from different basins deserves further
attention.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Turcotte:2023:POG,
author = "Lenora D. M. Turcotte and Julia C. Bradshaw and Mark
P. Polinski and Stewart C. Johnson",
title = "\bioname{Piscine orthoreovirus} Genotype-1 ({PRV-1})
in Wild \geoname{Pacific} Salmon of \geoname{British
Columbia, Canada}: 2011--2020",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "252",
day = "10",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050252",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/252",
abstract = "Piscine orthoreovirus genotype 1 (PRV-1) is an endemic
virus to the Northeastern Pacific that infects both
wild and farmed salmon. To better understand PRV-1
prevalence and transmission pathways in the region, we
targeted out-migrating juvenile Pacific Salmon in the
Strait of Georgia and Johnstone Strait in British
Columbia, Canada, for PRV-1 molecular screening with an
emphasis on Chinook (Oncorhynchus tshawytscha) and Coho
(Oncorhynchus kisutch) salmon. A total of 4031
individuals were screened for PRV-1 and we identified
an overall virus prevalence of 3.5\% from 2011 to 2020.
PRV-1 was absent in enhancement facilities and
freshwater catchments and predominately found in the
marine environment. The detection of PRV-1 varied
greatly between species, year and stock of origin, but
cumulatively identified that life history and migratory
behaviors likely influenced viral prevalence.
Specifically, Chinook salmon, which demonstrate long
residence time in the Strait of Georgia relative to
other species, had the highest PRV-1 prevalence in this
study (7.4\%). Varying stock composition and regional
detection between year classes within the Strait of
Georgia further indicated that the sources for Chinook
infection were highly variable between years. These
findings enhance our understanding for PRV-1
transmission in the region and more generally
between/within salmon species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Heidarieh:2023:EID,
author = "Marzieh Heidarieh and Amin Gholamhosseini and Najmeh
Sheikhzadeh and Maria Angeles Esteban",
title = "Effects of $ \gamma $-Irradiated Date
(\bioname{Phoenix dactylifera}) Fruit on Growth,
Immunological and Antioxidant Parameters of Goldfish
(\bioname{Carassius auratus})",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "251",
day = "09",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050251",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/251",
abstract = "The aim of this study was to evaluate the potential
effects of {\gamma}-irradiated date palm fruit (Phoenix
dactylifera), which is rich in phenolic acids and has
important and varied biological bioactivities, on
growth performance, mucosal immunity and liver
antioxidant status in goldfish (Carassius auratus). The
antioxidant activity, alongside the phenolic and
flavonoid contents, were also determined in irradiated
palm fruit extracts (IPFE). The results showed that
IPFE5 had a higher antioxidant activity as well as
heightened phenolic and flavonoid contents compared to
IPFE10 and IPFE0. Meanwhile, goldfish specimens were
randomly divided into three groups with three
replicates in each. Subsequently, skin immunity and
antioxidant activity in the livers of the goldfish were
studied. The growth rates of fish fed with IPFE5 and
IPFE10 were significantly higher (p {$<$} 0.05)
compared to the CTR group. In addition, fish fed with
the IPFE5 diet demonstrated increased mucosal immunity
compared to the CTR group (p {$<$} 0.05). Lipid
peroxidation levels as well as antioxidant enzyme
activities were also higher in all the IPFE-fed groups
compared to the CTR group (p {$<$} 0.05). These data
showed that 40-day dietary administration of
{\gamma}-irradiated date extract, especially IPFE5,
improved growth performance, mucosal immunity, and
liver antioxidant capacity in goldfish. The suitability
of administrating this additive in the diet of farmed
fish is discussed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2023:IIS,
author = "Yeying Wu and Yalan Feng and Mingyang Xue and Zidong
Xiao and Lijuan Jin and Ren Gao and Yahong Chen and
Tianwang Liang and Yong Zhou",
title = "Isolation and Identification of
\bioname{Staphylococcus saprophyticus} from Diseased
Hybrid Sturgeon",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "250",
day = "09",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050250",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/250",
abstract = "Hybrid sturgeon is an important economic fish species
in China. In 2021, a bacterium was isolated from the
liver and kidneys of freshwater-farmed hybrid sturgeon
in Yichang City, Hubei Province, causing a disease with
high mortality and surface bleeding. Through
morphological observation, 16S rDNA gene sequence
analysis, pathogenicity, an antimicrobial sensitivity
test, as well as serum physiological and biochemical
analysis, it was identified as Staphylococcus
saprophyticus and named E702. The 16S rDNA gene
sequence of E702 is highly homologous to S.
saprophyticus in GenBank. Phylogenetic analysis showed
that E702 and S. saprophyticus clustered into one
clade. The 50\% lethal dose of E702 was 2.14 $ \times $
10 5 CFU/g. The percentages of monocytes and
eosinophils were markedly increased in the diseased
sturgeon's blood, whereas the percentages of platelets
and lymphocytes were decreased. The activity levels of
aspartate aminotransferase, alanine aminotransferase
and alkaline phosphatase in the diseased fish were
significantly increased. The diseased fish suffered
obvious damage to many tissues and organs, especially
the liver and kidney, showing swelling, hyperemic and
inflammatory cell infiltration. E702 was sensitive to
antibiotics such as neomycin, cefazolin, norfloxacin,
carbenicillin, gentamicin and ciprofloxacin. The study
not only proved that S. saprophyticus was responsible
for a great deal of hybrid sturgeon deaths, but also
shed light on its potential risks in hybrid sturgeon
farming. The research results provided the theoretical
basis for the diagnosis as well as prevention of this
disease.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:DDM,
author = "Kexin Zhang and Weiren Zhang and Ronghua Li and Junkai
Lu and Qingwei Chen and Haojie Hu and Fei Yin and
Changkao Mu and Weiwei Song and Chunlin Wang",
title = "Dynamic Distribution of \bioname{Mesanophrys} sp. and
Tissue Enzyme Activities in Experimentally Infected Mud
Crab \bioname{Scylla paramamosain}",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "249",
day = "08",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050249",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/249",
abstract = "Mesanophrys sp. is reported to be highly pathogenic to
marine crustaceans. This study presents the first
report of Mesanophrys sp. infection in the mud crab
(Scylla paramamosain). In this study, we first recorded
the survival rates of an experimentally infected group
and a control group; the cumulative survival rate in
the infected group was significantly lower compared to
the control group after 72 h (73.20\% vs. 94.19\%),
while the highest mortality of S. paramamosain occurred
within the first 24 h post-infection. Then, we
investigated the dynamic distribution and tissue
tropism of the Mesanophrys sp. in the infected S.
paramamosain by a quantitative real-time polymerase
chain reaction (qPCR). The result showed that a
significant increase in the number of Mesanophrys sp.
could be detected in all tested tissues (obtained from
the eyestalks, gills, heart, nerves, muscles and
hepatopancreas) at 3 h post-infection. The numbers of
Mesanophrys sp. in the gill, eyestalk and nerve tissues
were relatively higher than in the other tissues. The
gill tissue showed the highest numbers from 6 to 48 h.
Histopathological observation found a severe collapse
in the filament structure, which indicated
tissue-specific pathogen infection. Furthermore, the
antioxidant enzyme activity of superoxide dismutase
(SOD), catalase (CAT) and peroxidase (POD) in three
representative tissues (gill, muscle and
hepatopancreas) were compared between the infected and
control groups, and a significant increase in enzyme
activity was observed in all three tested tissues in
the infected group, indicating a relatively strong
innate immune defense reaction that could have been
induced by Mesanophrys sp. infection. These results
will be helpful to Mesanophrys sp.
pathogenicity-related research and the control of this
pathogen in S. Paramamosain in the future.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Han:2023:AAE,
author = "Chenglong Han and Kefeng Liu and Toshihisa Kinoshita
and Biao Guo and Yifan Zhao and Yuhang Ye and Yufei Liu
and Osamu Yamashita and Debin Zheng and Wenhui Wang and
Xueqiang Lu",
title = "Assessing the Attractive Effects of Floating
Artificial Reefs and Combination Reefs on Six Local
Marine Species",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "248",
day = "08",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050248",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/248",
abstract = "Artificial reefs (ARs) have been advocated for and
implemented as management tools for recreational
fisheries, species conservation, and habitat
replacement; however, the research that includes
attracting marine species of floating ARs remains in
its early stages. Here, two types of floating ARs were
designed to evaluate the attractive effects using the
occurrence rate and attracting index for six
commercially important species (Lateolabrax maculatus,
Liza haematocheila, Sebastes schlegelii, Acanthopagrus
schlegelii, Litopenaeus vannamei, and Amphioctopus
fangsiao) in the Bohai Bay of China; their combined ARs
were meanwhile compared with two variants of artificial
seagrass beds (SA and SB) and the traditional
double-frame artificial reef (TD). All of the designed
ARs were effective in attracting experimental species.
The ARs with higher shelter areas (SB and TD) showed a
better attracting effect. The efficiency of the ARs in
attracting different species depended on their life
histories. The bottom-mounted ARs were more efficient
in attracting demersal species, while the floating ARs
attracted epipelagic fish. In addition, the combined
reefs had a better attractive effect than single ARs
did. Overall, floating ARs and their combined ARs show
the potential to deploy especially for attracting
epipelagic fishes, although further study is needed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jang:2023:EHA,
author = "Won Je Jang and Shin-Kwon Kim and So Young Park and
Dong Pil Kim and Yun-Jy Heo and Haham Kim and Su-Jeong
Lee and Min Gyu Shin and Eun-Woo Lee and Seunghyung Lee
and Jong Min Lee",
title = "Effect of Host-Associated Bacillus-Supplemented
Artificial Diets on Growth, Survival Rate, and Gene
Expression in Early-Stage Eel Larvae (\bioname{Anguilla
japonica})",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "247",
day = "08",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050247",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/247",
abstract = "Beneficial microorganisms can increase nutrient
digestion and absorption in farmed fish. This study
investigates the effects of supplemental feeding of
Bacillus species isolated from the intestines of wild
glass eels on the growth, survival, and gene expression
of farm-raised eel larvae for 30 days after hatching.
Three species of Bacillus (B. velezensis, AJBV; B.
subtilis, AJBS; B. licheniformis, AJBL) without
hemolytic activity were isolated, and an experiment
compared the growth of eel larvae fed an artificial
diet supplemented with each Bacillus species. There
were no significant differences in the total length and
body depth of eel larvae at 30 days after hatching in
all groups. During the feeding period, 149 eels
survived from the initial 1000 in the control group. On
the other hand, 240, 178, and 141 eels survived in the
AJBV, AJBS, and AJBL groups, respectively. However,
there were no significant differences in survival rates
despite the difference in the number of surviving eel
larvae among the groups. In the comparison of gene
expression of genes involved with growth (growth
hormone, growth hormone receptor 1, insulin-like growth
factor II-2) and those involved with digestive enzymes
(amylase, trypsin, lipase), there were also no
significant differences among the groups. Our results
confirm that dietary supplementation with each of the
three host-associated Bacillus does not affect the
growth and survival rates of eel larvae reared on an
artificial diet up to the first 30 days after hatching,
nor does it significantly affect related gene
expression.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esparza-Espinoza:2023:CSI,
author = "Dania Marisol Esparza-Espinoza and Hisila del Carmen
Santacruz-Ortega and Maribel Plascencia-Jatomea and
Santiago P. Aubourg and Jes{\'u}s Aar{\'o}n
Salazar-Leyva and Francisco Rodr{\'\i}guez-Felix and
Josafat Marina Ezquerra-Brauer",
title = "Chemical-Structural Identification of Crude Gelatin
from Jellyfish (\bioname{Stomolophus meleagris}) and
Evaluation of Its Potential Biological Activity",
journal = j-FISHES,
volume = "8",
number = "5",
pages = "246",
day = "08",
month = may,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8050246",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:42 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/5/246",
abstract = "The demand for jellyfish is growing worldwide,
especially due to their high nutraceutical value. In
this study, the extraction and characterization of
crude gelatin from the brown cannonball jellyfish
(Stomolophus meleagris), which is periodically found in
large volumes on the American Pacific coasts, were
carried out. The crude gelatin obtained by alkaline
treatment, with subsequent heat and dialysis treatment,
showed an ability to quench free radicals (via ABTS and
ORAC methods), and protect human cells against
oxidative damage (through inhibition of hemolysis by
AAPH), and they protected against mutations caused by
aflatoxin B 1 in the Salmonella enterica Typhimurium
TA100 strain. Furthermore, it was established that
these extracts were innocuous for eukaryotic cells
(genotoxicity assay). The amino acid profiles indicate
a high concentration of glycine and proline, as well as
charged amino acids. Electrophoretic, FT-IR, and 1
H-NMR studies indicated that one of the main proteins
present in this crude gelatin is collagen. The presence
of collagen and other proteins was identified by
proteomic studies. Alkaline crude gelatin from brown
jellyfish could be considered as potential candidates
to be evaluated as antioxidant agents in foods in
future research.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Finnas:2023:POM,
author = "Viktor Finn{\"a}s and Erkki Jokikokko and Jan-Olof
Lill and Yann Lahaye and Henry H{\"a}gerstrand and Kai
Lindstr{\"o}m",
title = "Potential of Otolith Microchemistry to Distinguish
Nursery Areas of Salmon within \geoname{River
Simojoki}",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "332",
day = "20",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060332",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/332",
abstract = "Draining into the northern Baltic Sea, River Simojoki
is an important spawning river for Atlantic salmon. The
present study aimed to preliminary explore the
potential of analysing the elemental composition of
otoliths to distinguish the within-river nursery area
of origin for salmon. Parr were sampled at three
nursery areas in the river and smolts of unknown origin
were sampled near the river mouth during the migration
to the sea. The concentrations of multiple elements and
the strontium 87 Sr/ 86 Sr isotopic ratio in the
otoliths were analysed using single- and
multi-collector laser ablation inductively coupled
plasma mass spectrometry, respectively. Based on the
otolith elemental variables, parr could be reclassified
to the sampled nursery areas using discriminant
function analysis and random forest with a success rate
of 53.3\% and 63.3\%, respectively. However, for parr
sampled at the uppermost nursery area in the river, the
success rates were 90\% and 100\%, respectively.
Applying the classification models trained on the parr
samples to determine which nursery area sampled smolt
originated from was constrained by the limited sampling
of parr, both in sample sizes and the coverage of the
nursery areas found in the river.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kovacic:2023:SRG,
author = "Marcelo Kova{\v{c}}i{\'c} and Sergio
Ram{\'\i}rez-Amaro and Maria Teresa Farriols and
Francesc Ordines",
title = "The Second Record of \bioname{Gymnesigobius medits}
{Kova{\v{c}}i{\'c}}, {Ordines, Ramirez-Amaro \&
Schliewen}, 2019, the Deepest Benthic Gobiiform
Species, and the Additional Records of \bioname{Gobius
xoriguer} {Igl{\'e}sias, Vuki{\'c} \& {\v{S}}}anda,
2021 ({Actinopterygii: Gobiiformes: Gobiidae})",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "331",
day = "20",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060331",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/331",
abstract = "Gymnesigobius medits is reported for the first time
after a recent description recorded from the Balearic
Islands and from the slope of the Gulf of Vera on the
Iberian Peninsula coast. The record from the Emile
Baudot seamount on the Balearic Islands represents the
deepest positive benthic gobiiform species record in
general. The presence of the membrane connection
between the pelvic fins in Gymnesigobius medits,
presumed on the damaged fin in the original
description, was confirmed. The recently described
Gobius xoriguer is the first record from the Pitiusas
and Columbretes islands and from the Iberian Peninsula
coast. It appears to be widely distributed in the
circalittoral bottoms, preferentially in red algae
beds. Morphological identification of both species was
confirmed using molecular analyses based on the
sequencing of the mitochondrial cytochrome c oxidase
subunit I (DNA barcode) gene. The deepest records of
gobiiform fishes in oceans and seas are reviewed. The
European seas, a well-studied area with eight gobiid
species recorded deeper than 200 m, show high bathyal
gobiid species richness compared to other areas. The
real worldwide diversity of bathyal gobies, although
only a fraction of the shallow water species richness
of this taxon, is probably much larger than presently
known.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tefal:2023:NOR,
author = "Eslam Tefal and Ignacio Jauralde and Ana
Tom{\'a}s-Vidal and Silvia Mart{\'\i}nez-Llorens and
David S. Pe{\~n}aranda and Miguel Jover-Cerd{\'a}",
title = "New Organic Raw Materials for Gilthead Seabream
(\bioname{Sparus aurata}) Feeding and the Effects on
Growth, Nutritive Parameters, Digestibility, and
Histology",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "330",
day = "20",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060330",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/330",
abstract = "A 120-day experiment investigated the new organic raw
materials for Gilthead seabream (Sparus aurata) feeding
on growth, nutritional parameters, digestibility, and
histology. An organic control diet (CON) and three
diets with 100\% organic raw materials --- the rest of
rainbow trout, visceral Iberian pig, and insects as a
protein source (TRO, IBE, and INS) --- were tested.
After the experiment, growth, nutritional parameters,
digestibility, and histology were measured. The CON
diet-fed seabream obtained the highest weight; there
was no difference between the experimental diets. The
crude protein content was the highest in seabream fed
the TRO and INS diets. Crude fat was the highest value
observed in the CON diet. High digestibility was
observed in seabream-fed IBE and INS diets. Except for
EAA methionine (Met), there were no static differences
in retention efficiency. The diet with the highest
hydrolysis rate was IBE. Diet differences were
significant but had the typical healthy liver
morphology of seabream. Seabream fed on the TRO and INS
diets had shorter distal gut measurements. In
conclusion, the full substitution of fishmeal with
organic raw materials, including rainbow trout remains,
Iberian pig viscera, and insects, offers several
benefits in terms of digestibility, histology, and
growth performance and may help improve sustainable and
healthy aquaculture practices.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:EDL,
author = "Jiaming Xu and Zhoulin Yu and Guangye Liu and Sijie Li
and Guoyong Zhou and Hanhua Wang and Yewei Dong and
Cuihong You and Weidong Bai and Meng Zhou and Yanhua
Huang and Xiaohong Tan",
title = "Effects of Dietary \bioname{Lentinus edodes}
Fermentation Supplementation on Digestive Enzyme
Activity, Antioxidant Capacity and Morphology of the
Liver and Intestine in Largemouth Bass
(\bioname{Micropterus salmoides}) Fed High Plant
Protein Diets",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "329",
day = "20",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060329",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/329",
abstract = "This study evaluated the effect of Lentinus edodes
fermentation (LEF) on digestive enzyme activity,
antioxidant capacity and morphology of the liver and
intestine in largemouth bass (Micropterus salmoides)
fed high plant protein diets (HPPD). LEF was
supplemented in HPPD with 0 g kg {$-$1} (LEF0), 10 g kg
{$-$1} (LEF1), 20 g kg {$-$1} (LEF2), 30 g kg {$-$1}
(LEF3), 40 g kg {$-$1} (LEF4), 50 g kg {$-$1} (LEF5),
respectively, and then the six diets were fed to
largemouth bass with a body weight of 28.8 {\pm} 0.05 g
for eight weeks. Juvenile fish were randomized into 6
groups and each group had 4 replicates with 40 fish.
Dietary LEF supplementation alleviated the liver
inflammatory reaction of largemouth bass caused by HPPD
and improved liver morphology. Goblet cells multiplied
and the gut muscle layer thickened after LEF
supplementation. The LEF significantly increased
amylase activity in the liver and intestine of
largemouth bass in individual experimental groups. The
LEF could increase the activity of catalase in the
liver and intestine of largemouth bass (p {$<$} 0.05).
The content of malondialdehyde was significantly lower
than that in the control group (p {$<$} 0.05). Dietary
LEF supplementation had no significant effect on the
intestinal flora of largemouth bass. These findings
imply that LEF supplementation can reduce liver
inflammation, enhance intestinal tissue morphology, and
eventually benefit largemouth bass health.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lassauce:2023:SEP,
author = "Hugo Lassauce and Olivier Chateau and Laurent
Wantiez",
title = "Spatial Ecology of the Population of Reef Manta Rays,
\bioname{Mobula alfredi} ({Krefft}, 1868), in
\geoname{New Caledonia} Using Satellite Telemetry 1 ---
Horizontal Behaviour",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "328",
day = "19",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060328",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/328",
abstract = "The reef manta ray, Mobula alfredi (Krefft, 1868), is
a highly mobile and plankton-feeding species,
classified vulnerable to extinction on the IUCN Red
List for Threatened Species. Knowledge on their spatial
ecology and the extent of their dispersal remain
incomplete, especially within island-fragmented
habitats as found in New Caledonia. Satellite telemetry
was used to investigate the horizontal movement ecology
of reef manta rays in New Caledonia. A total of 21 reef
manta rays were tagged with pop-up satellite archival
transmitting tags (21 Fastloc and 2 MiniPAT) that
remained deployed for a duration ranging from 3 to 180
days (mean {\pm} SE = 76.7 {\pm} 50.3). Rays presented
a strong site fidelity and an important affinity for
coastal waters. Long-distance migrations ({$>$300} km)
were also observed, mainly through coastal and shallow
water paths. Horizontal movements were compared to a
home range area and classified into four distinct
patterns: Fidelity, Excursion, Fidelity + Relocation
and Relocation. The most dominant pattern was Fidelity,
where manta rays remained within their home range for
the whole duration of the tag deployment. Our findings
may assist in the design of more appropriate management
strategies for the species in New Caledonia and other
regions worldwide.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chaiyasut:2023:DAP,
author = "Kasidis Chaiyasut and Paul Merviel and Peter Palma and
Johanna Perschthaler and Eduardo Jimenez-Fernandez and
Andrew Davie and Alejandro P. Gutierrez",
title = "Development of an Accurate Polymerase Chain Reaction
({PCR}) Assay for Genetic Sex Identification in
Lumpfish (\bioname{Cyclopterus lumpus}) Based on
Male-Specific Anti-Mullerian Hormone (\gene{amh})
Gene",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "327",
day = "19",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060327",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/327",
abstract = "The production of lumpfish (Cyclopterus lumpus) has
become crucial in controlling sea lice levels in
salmonid aquaculture. To improve their breeding, there
is a need for early sex identification. The genomic
region containing the anti-M{\"u}llerian hormone (amh)
gene was suggested as the candidate master
sex-determining gene in lumpfish. However, the genome
of lumpfish contains three copies of amh with ambiguous
sex specificity, designated amh1, amh2, and amh3. The
study aims to analyse the male-specific region between
these amh paralogues for its application as a sex
marker. In this study, we utilised polymerase chain
reaction (PCR)-based assays to identify the
male-specific amh markers in lumpfish and estimate the
length of the male-specific region in the lumpfish
genome. Our results indicate that a specific genomic
region of approximately 27 kilobases (kb), encompassing
amh1 and amh2 genes, exhibits male specificity, whereas
amh3 is present in both sexes. The developed PCR-based
genetic sex identification assays targeting amh1 and
amh2 exhibited over 97\% concordance with phenotypic
records. Further experiments in other members of the
Cyclopteridae: Aptocyclus ventricosus, Eumicrotremus
taranetzi, and E. asperrimus revealed male-specific amh
genome region only in A. ventricosus. Phylogenetic
analyses using the available Cyclopteridae amh
sequences suggest that male-specific amh arose early in
the Cyclopteridae lineage. Our findings, along with the
development of the PCR test, hold great promise for the
field of lumpfish aquaculture and will also contribute
significantly to future investigations aiming to
enhance our understanding of the sex-determination
system and the evolution of sex chromosomes in
teleostean fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hoseinifar:2023:EGV,
author = "Seyed Hossein Hoseinifar and Zohreh Fazelan and Ehab
El-Haroun and Morteza Yousefi and Metin Yazici and Hien
Van Doan and Marina Paolucci",
title = "The Effects of Grapevine (\bioname{Vitis vinifera}
{L}.) Leaf Extract on Growth Performance, Antioxidant
Status, and Immunity of Zebrafish (\bioname{Danio
rerio})",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "326",
day = "19",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060326",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/326",
abstract = "An 8-week feeding trial was carried out to evaluate
the effects of grapevine (Vitis vinifera) leaf extract
(GLE) on the growth, oxidative enzymatic activities,
immunity, and expression of antioxidant genes in
zebrafish (Danio rerio). Three hundred and sixty
zebrafish were supplied and fed with different levels
of GLE: 0, 0.5, 1, and 2 g kg {$-$1}. The dietary
administration of 1 g kg {$-$1} of GLE significantly
increased growth parameters in fish. Fish fed diets
with GLE showed increased total protein. The total Ig
and lysozyme activity significantly changed in the
whole-body serum, but not in skin mucus. GLE
significantly increased Catalase (CAT), Superoxide
Dismutase (SOD), and Glutathione Peroxidase (GPx)
activities compared to the control diet. GLE treatments
caused a significant decrease in the malondialdehyde
(MDA) content. In the skin mucus, only CAT and SOD
activities significantly increased. The highest
expression of Toll-like receptor-1 (TLR-1) and Tumor
Necrosis Factor-{\alpha} (TNF{\alpha}) genes was
achieved in fish fed 2 g kg {$-$1} of GLE. CAT and SOD
gene expressions were significantly higher in fish fed
1 and 2 g kg {$-$1} of GLE. GPx gene expression was
significantly higher in fish fed 1 g kg {$-$1} of GLE.
In conclusion, the results of the present study
revealed that GLE affects growth performance and
regulates antioxidant and immune gene expression. The
determination of the optimum dosage merits further
research.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jimenez-Ruiz:2023:QIS,
author = "Edgar Iv{\'a}n Jim{\'e}nez-Ru{\'\i}z and V{\'\i}ctor
Manuel Oca{\~n}o-Higuera and Santiago Valdez-Hurtado
and Jos{\'e} Alberto Cruz-Guzm{\'a}n and Cesar
Benjam{\'\i}n Otero-Le{\'o}n and Sa{\'u}l
Ru{\'\i}z-Cruz and Alba Mery Garz{\'o}n-Garc{\'\i}a and
Hebert Jair Barrales-Cure{\~n}o and Dalila Fernanda
Canizales-Rodr{\'\i}guez and Cinthia Jhovanna
P{\'e}rez-Mart{\'\i}nez and Mar{\'\i}a Teresa
Sumaya-Mart{\'\i}nez",
title = "Quality Improvement and Shelf-Life Extension of Iced
\geoname{Nile} Tilapia Fillets Using Natural Garlic
Extract",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "325",
day = "19",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060325",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/325",
abstract = "Fish represent one of the most perishable food groups.
Therefore, it is important to find viable alternatives
that contribute to the preservation of quality and
increase the shelf life of fishery products, and one
alternative is to use natural extracts with
antimicrobial activity. The objective of this study was
to determine the effect of a natural extract prepared
with garlic (NGE) on the quality and shelf life of
tilapia fillets stored on ice for 18 days. For this
purpose, NGE was prepared by homogenizing peeled garlic
cloves with distilled water, which were then
centrifuged to obtain the extract (NGE); then, the fish
fillets were immersed in the extract and were coated in
NGE. The fillets with NGE were packed in high-density
polyethylene bags and stored in crushed ice for 18
days. The adenosine 5{\prime}-triphosphate (ATP) and
degradation products, K-value, color, texture, water
holding capacity, pH, total mesophilic count, and total
volatile bases (TVB-N) were monitored during storage.
The ATP content, K-value, pH, total microbial count,
and TVB-N changed with respect to ice storage time, and
the results between fillets with NGE and control
fillets differed. In conclusion, the application of NGE
increased the shelf life of fillets stored on ice by 6
days, obtaining a shelf life of 18 days on ice, which
shows its potential to improve the utilization of the
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ramos:2023:WFP,
author = "Jorge Ramos and Pedro G. Lino and Jaime An{\'\i}bal
and Eduardo Esteves",
title = "{(Un)wanted} Fish: Potential Consumers' Acceptability
of Landings in the \geoname{Portuguese} Case",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "324",
day = "18",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060324",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/324",
abstract = "The Landing Obligation (LO), introduced in 2015 by the
Common Fisheries Policy of the European Union (EU-CFP),
has been subject to a transitional period until
recently. The rationale behind the measure is that all
fish species subject to a total allowable catch (TAC)
must be landed to increase the sustainability of
fishing activities. Through the analysis of official
statistical data, it is possible to find out which
species of fish were landed and their relative
importance, including their monetary value, and verify
the potential for consumer acceptance. Some insights
are drawn from the interconnection between these three
factors (i.e., social acceptability, landings of main
fish species, and their market value) with empirical
results and the scientific literature using data from
Portugal.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Watz:2023:ITD,
author = "Johan Watz and Joel Schill and Louis Addo and John J.
Piccolo and Mahboobeh Hajiesmaeili",
title = "Increased Temperature and Discharge Influence
Overwinter Growth and Survival of Juvenile Salmonids in
a Hydropeaking River: Simulating Effects of Climate
Change Using Individual-Based Modelling",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "323",
day = "18",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060323",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/323",
abstract = "Climate change causes warming of rivers and may
increase discharge, particularly during winter.
Downstream of hydropower plants, fluctuating water
temperature and flow create dynamic overwintering
conditions for juvenile salmonids. We used inSTREAM
7.2-SD to simulate the effects of increased temperature
(+2 {$^\circ $}C) and discharge (+10\%) on the
overwinter growth and mortality of one-summer- and
two-summer-old Atlantic salmon and brown trout in a
river with a hydropeaking flow regime in a 2 $ \times $
2 design with replicated simulations. Water temperature
had a major positive relationship with growth for both
species and year classes, whereas increased flow alone
had no major general effect on overwinter growth. For
one-summer-old trout experiencing the high temperature
regime, however, increased flow resulted in reduced
growth. There were no major effects from temperature
and flow on the survival rate of the two-summer-old
fishes. On the other hand, there were significant
interaction effects for the one-summer-olds, indicating
that the effect of flow depended on temperature. For
one-summer-old salmon, high flow resulted in increased
survival in the low temperature regime, whereas it
resulted in reduced survival in high temperature. In
contrast, for one-summer-old trout, high flow resulted
in reduced survival in the low temperature regime and
increased survival in the high temperature. Different
hydropower operation alternatives may interact with
warming, affecting the relative competitive abilities
of stream salmonids. Ecological models that predict the
effects of different environmental conditions, such as
temperature and flow regimes, may offer insight into
such effects when in situ experiments are not
feasible.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dayrit:2023:PIS,
author = "Geraldine B. Dayrit and Emmanuel M. Vera Cruz and
Channarong Rodkhum and Mahmoud Mabrok and Pattareeya
Ponza and Mudjekeewis D. Santos",
title = "Potential Influence of Shading in Freshwater Ponds on
the Water Quality Parameters and the Hematological and
Biochemical Profiles of \geoname{Nile} tilapia
(\bioname{Oreochromis niloticus} {Linnaeus}, 1758)",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "322",
day = "17",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060322",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/322",
abstract = "Nile tilapia is a high-demand commodity in most
developing countries including the Philippines, but its
production is hampered by fluctuating water temperature
(WT) and dissolved oxygen (DO) concentrations, which
are mostly influenced by temperature warming. Net
shading has been demonstrated to reduce pond
temperature and increase the rate of spawning in
cultured tilapia. The current study aimed to
investigate the potential effects of shading on the
water quality parameters, blood indices, and serum
biochemical profiles of cultured Nile tilapia. Fish
were collected from unshaded (control) and shaded
(experimental) ponds in three breeding cycles. WT and
DO concentrations were measured, while hematological
and biochemical analyses were performed. The results
showed that the average WT was considerably decreased
in the shaded pond with no significant difference in
the DO concentrations. Red blood cells, hemoglobin, and
lymphocyte counts were significantly lower in fish
raised in shaded ponds, although mean corpuscular
volume and neutrophil counts were significantly higher.
Likewise, Chole, TP, and Alb values were significantly
affected by the interaction of cycle and shading setup.
Cortisol, Glb, K +, Cl {$-$}, Mg 2+, and TCa values
showed significant differences and were only affected
by the cycle. Similarly, AST and ALT values showed
significant differences and were affected by both cycle
and shading setup. Water quality parameters (WT and
DO), hematological blood indices, and serum biochemical
variables were correlated positively with each other.
In brief, prior data imply that net shading can reduce
WT in aquaculture ponds, reduce stress in Nile tilapia,
and lessen the consequences of temperature warming on
species production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Almodovar:2023:BAS,
author = "Ana Almod{\'o}var and Graciela G. Nicola and Daniel
Ayll{\'o}n and Sheila Leal and Daniel F. March{\'a}n
and Benigno Elvira",
title = "A Benchmark for \geoname{Atlantic} Salmon
Conservation: Genetic Diversity and Structure in a
\geoname{Southern European} Glacial Refuge before the
Climate Changed",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "321",
day = "16",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060321",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/321",
abstract = "Atlantic salmon Salmo salar supports highly valuable
commercial and recreational fisheries in Europe, but
its stocks are currently overexploited and threatened
by climate change. Its southernmost populations (in
northern Spain) play a key role in conserving the
species' original genetic diversity, which is
endangered due to decades-long (1970s to 1990s) massive
stocking with non-native stocks. Their decline is well
documented, but the effect of stock transfer and
conservation efforts is unclear. Nine microsatellite
loci were amplified from archival samples (scales from
1958--1959) from eight Spanish rivers to analyse the
species' natural genetic dynamics before its decline
started. Allelic richness was high in the historical
populations (the 1950s) and above most contemporary
estimates. Private alleles were found in most rivers,
indicating high local uniqueness and relative isolation
among river basins. Some alleles are regional markers
since they are rare or absent from contemporary
northern European populations. Effective population
size suggested good conservation status, with higher
values than those estimated for contemporary
populations. Strong population structure and genetic
differentiation between rivers were found, with limited
gene flow, restricted to geographically close
populations. Our estimates of historical genetic
diversity and structure from southernmost salmon
populations are a powerful benchmark to guide
conservation programs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gauy:2023:BTS,
author = "Ana Carolina dos Santos Gauy and Marcela Cesar
Bolognesi and Eliane Gon{\c{c}}alves-de-Freitas",
title = "Body Tactile Stimulation Reduces the Effects of High
Stocking Density on the Welfare of \geoname{Nile}
Tilapia (\bioname{Oreochromis niloticus})",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "320",
day = "16",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060320",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/320",
abstract = "High stocking density is used in aquaculture to
optimize farming. However, such strategies can stress
territorial fish by increasing aggression, thus harming
the fish welfare and productive performance. Here, we
tested the effect of long-term tactile body stimulation
(TS) on alleviating the impact of high stocking density
in Nile tilapia (Oreochromis niloticus). Ten males were
grouped for 21 days into four treatments (10 replicates
each one) combining TS with high (1.2 kg{\cdot}m{$^3$})
or low (0.6 kg{\cdot}m{$^3$}) densities: (1) without TS
and high density; (2) without TS and low density; (3)
with TS and high density; and (4) with TS and low
density. A rectangular PVC frame fitted with vertical
plastic sticks sided with silicone bristles was placed
in the center of aquarium. The fish receive TS when
passing through the bristles. An apparatus without
bristles was used as control. We found higher cortisol
levels under TS conditions. All parameters of
productive performance (growth, food efficiency, and
condition factor) were higher in the treatment with TS
and low density, lower in the treatment without TS and
high density, and similar between treatments high
density with TS and low density without TS. The results
suggest that TS improves productive performance of Nile
tilapia, counteracting the negative effects of high
stocking density.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Crozier:2023:CRI,
author = "Lisa G. Crozier and Jared E. Siegel",
title = "A Comprehensive Review of the Impacts of Climate
Change on Salmon: Strengths and Weaknesses of the
Literature by Life Stage",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "319",
day = "16",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060319",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/319",
abstract = "As we confront novel environmental challenges, a full
understanding of the physical and biological processes
that govern species responses to climate change will
help maintain biodiversity and support conservation
measures that are more robust to irreducible
uncertainty. However, climate impacts are so complex,
and the literature on salmon and trout is so vast that
researchers and decision makers scramble to make sense
of it all. Therefore, we conducted a systematic
literature review of climate impacts on salmon and
anadromous trout as a resource for stakeholders,
managers, and researchers. We reviewed studies
published from 2010 to 2021 that address climate
impacts on these fish and organized them in a database
of 1169 physical and 1853 biological papers. Papers are
labeled with keywords across eight categories related
to subject matter and study methods. We compared the
literature by biological process and life stage and
used these comparisons to assess strengths and
weaknesses. We then summarized expected phenotypic and
genetic responses and management actions by life stage.
Overall, we found the largest research gaps related to
species interactions, behavioral responses, and effects
that carry over across life stages. With this
collection of the literature, we can better apply
scarce conservation resources, fill knowledge gaps, and
make informed decisions that do not ignore
uncertainty.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barreiros:2023:WAG,
author = "Jo{\~a}o Pedro Barreiros and Felicia C. Coleman",
title = "\geoname{West African} Goliath Grouper: Where Are They
between \geoname{Senegal} and \geoname{Angola}?",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "318",
day = "16",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060318",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/318",
abstract = "The Atlantic Goliath Grouper Epinephelus itajara
(Lichtenstein, 1822) occurs on both sides of the
Atlantic, from the Carolinas (USA) to Brazil in the
western Atlantic and historically from North Senegal to
North Angola off of West Africa in the eastern
Atlantic. While there are relatively good data on their
distribution in the western Atlantic, confirmed
occurrences, population status, fishing reports, and
trade data are almost non-existent for West Africa.
Part of the problem is that tropical West African
countries largely lack the wherewithal to fund the
research needed to evaluate this species, nor do they
have laws, management plans, or viable enforcement
measures that might lead to effective population
recovery or protection for essential juvenile mangrove
habitats. Given the lack of published studies on this
species in West Africa, the primary objectives of this
paper are (1) to describe all known historical and
current anecdotal information available on this species
and (2) to encourage the increased monitoring of
habitats where viable populations might still occur
(i.e., mangroves, oil rigs, and oil platforms).",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Munoz-Lechuga:2023:DSU,
author = "Rub{\'e}n Mu{\~n}oz-Lechuga and Fambaye Ngom Sow and
Diaha N'Guessan Constance and Davy Angueko and David
Mac{\'\i}as and Alexia Massa-Gallucci and Guelson
Batista da Silva and Jorge M. S. Gon{\c{c}}alves and
Pedro G. Lino",
title = "Differentiation of Spatial Units of Genus
\bioname{Euthynnus} from the \geoname{Eastern Atlantic}
and the \geoname{Mediterranean} Using Otolith Shape
Analysis",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "317",
day = "15",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060317",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/317",
abstract = "The shape of sagitta otoliths was used to compare
individuals of little tunny (Euthynnus alleteratus)
harvested on board commercial fishing vessels from the
coastal areas along the Eastern Atlantic, including the
Mediterranean Sea. Fish sampling and selection was
designed to cover possible seasonal changes and tuna
size. The research encompassed both morphometric and
shape analyses of left sagittal otoliths extracted of
504 fish specimens. Four shape indices (Circularity,
Roundness, Rectangularity, and Form-Factor) were
significantly different between two groups, showing a
statistical differentiation between two clear spatial
units. The degree of divergence was even more
pronounced along the rostrum, postrostrum, and excisura
of the generated otolith outlines between these two
groups. One group corresponds to the samples from the
coastal areas in the Northeast Temperate Atlantic and
Mediterranean Sea (NETAM Area) and a second group from
the coastal areas off the Eastern Tropical Atlantic
coast of Africa (ETA Area). This study is the first to
use otolith shape to differentiate tunas from separate
spatial units. These results could be used to
re-classify previously collected samples and to correct
time series of data collected.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2023:SRI,
author = "Hongsik Kim and Ana C. Franco and U. Rashid Sumaila",
title = "A Selected Review of Impacts of Ocean Deoxygenation on
Fish and Fisheries",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "316",
day = "14",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060316",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/316",
abstract = "Oxygen is crucial for the survival of marine species.
Yet, the ocean has experienced a loss of approximately
2\% of its oxygen inventory since the last century,
resulting in adverse impacts on marine life and
ecosystems. In particular, changes in the gap between
the supply and demand for dissolved oxygen lead to
physiological and ecological variations, which cause
alterations in habitats and food webs for fish and
ecosystem services. These changes vary over time and by
region, and the heterogeneous characteristics of marine
species bring about non-linear consequences to human
society. Despite this, identifying the potential ripple
effects of deoxygenation on human society is
challenging due to the integrated impacts of other
stressors, such as global warming and ocean
acidification, and their varying changes depending on
environmental conditions and regions, such as upwelling
and eutrophication. Therefore, we conducted a
literature review on ocean deoxygenation and its
effects on fish dynamics and the ecosystem, with a
focus on the environmental and societal impact, to
present crucial considerations and pathways for future
research on ocean deoxygenation. We found that
quantitative approaches are necessary to assess the
dynamic changes under deoxygenation, and the consequent
effects on marine ecosystems should be verified to
exploit the natural resources from the ocean. One of
the most reliable approaches to quantifying the ripple
impacts of deoxygenation is to model spatial and
temporal changes with other climate stressors, forming
a global network encompassing socio-economic and
regional effects of this global change to facilitate
and improve capabilities to address the impacts of
ocean deoxygenation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Anderson:2023:CAB,
author = "Cody M. Anderson and Brian D. Wisenden and Cody A.
Craig and Craig A. Stockwell",
title = "Consistent Antipredator Behavioral Responses among
Populations of \geoname{Red River} Pupfish with
Disparate Predator Communities",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "315",
day = "13",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060315",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/315",
abstract = "Many desert fishes, which evolved in isolated aquatic
``islands'' with limited predation pressure, have been
severely impacted by non-native predators. These
impacts have been attributed to the evolutionary loss
of antipredator competence, known as the predator
naivet{\'e} hypothesis. Recent work provided support
for this hypothesis for one desert fish species. We
sought to examine the generality of the predator
naivet{\'e} hypothesis by evaluating antipredator
competence in five populations of Red River pupfish
(Cyprinodon rubrofluviatilis), a species that occupies
habitats that vary in the degree of isolation and
levels of fish species richness. Fish were exposed to a
conspecific chemical alarm cue released from damaged
epidermal tissue as a general assay of antipredator
response. We found that pupfish from all five
populations exhibited antipredator behavior in response
to the alarm cue, regardless of the exposure to
predation risk. These data provide evidence that
antipredator responses to alarm cues are conserved in
Red River pupfish, even in populations isolated from
piscivorous species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Batjakas:2023:LDC,
author = "Ioannis E. Batjakas and Athanasios Evangelopoulos and
Maria Giannou and Sofia Pappou and Eleftheria
Papanikola and Maria Atsikvasi and Dimitris Poursanidis
and Chrysoula Gubili",
title = "Lionfish Diet Composition at Three Study Sites in the
\geoname{Aegean Sea}: an Invasive Generalist?",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "314",
day = "13",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060314",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/314",
abstract = "The diet of the lionfish (Pterois miles), an invasive
species in the Aegean Sea, was examined by collecting
stomach content data from fish collected in three study
sites in the Aegean Sea (southern Crete, Kastellorizo,
and Nysiros islands). Prey composition in terms of
numerical abundance and frequency of occurrence was
used to compare lionfish's diet between these sites.
Lionfish largely preyed upon teleosts (4\% to 83\%
numerical abundance and 16\% to 58\% frequency of
occurrence, depending on the site) and decapods (12\%
to 95\% numerical abundance and 11\% to 81\% frequency
of occurrence). The most important teleost families in
lionfish's diet were Gobiidae, Labridae, and
Scorpaenidae, while decapods and especially the family
Scyllaridae and the genus Plesionika were the dominant
decapod prey items. The lionfish was found to be an
especially successful generalist across the study
sites, an opportunistic, predatory species overall, and
at the same time, at a local level, it seems to be an
equally successful specialist that could increase the
predation mortality of already stressed prey
populations and can be a serious threat to endemic,
critically endangered, and/or commercially important
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yu:2023:HIB,
author = "Tae-Sik Yu and Chang Woo Ji and Ihn-Sil Kwak",
title = "Hierarchical Interaction between Food Diversity and
Competition in Brackish Fish Species in \geoname{South
Korea}",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "313",
day = "13",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060313",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/313",
abstract = "Information about the food sources for fish is
important not only for predation and food competition,
but also for the direct impact on organism
interactions, relationships, and biodiversity within
aquatic ecosystems. We analyzed the food sources of 21
fish species in 9 families of 6 orders based on the
literature data from brackish ecosystems in South Korea
to improve the understanding of the estuarine
ecosystem. The food sources of the 21 fish species
contained 173 families, 86 orders, 39 classes, and 22
phyla. The 21 fish species were classified into 4
groups using hierarchical analysis based on their food
sources: Group 1 fishes mainly consumed Amphipoda;
Group 2 fishes were zooplanktivores; Group 3 were
omnivorous; and Group 4 consumed small food sources,
such as Nematoda, Corophiidae, and Harpacticidae. The
feeding competition index was relatively high within
each group, but the competition index for Group 3, the
omnivorous fishes, was low. Network analysis and hub
scores represented the importance of food sources
consumed by each fish species by showing the
connections between the fish and their prey.
Polychaeta, Actinopterygii, and Decapoda were the most
commonly consumed food sources. Food source diversity
was high for zooplanktivores (group 2). Additionally,
the food contents of each fish species were classified
into freshwater and marine sources for identifying the
habitats of the food sources. Trophic level measuring
values using the data were similar to the reported
literature data. This study can be utilized for
understanding estuarine ecosystems and identifying food
relationships.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Puvanendran:2023:SLT,
author = "Velmurugu Puvanendran and {\O}yvind J. Hansen",
title = "Short and Long-Term Effects of Early and Late Weaning
on \geoname{Atlantic} Cod, \bioname{Gadus morhua}",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "312",
day = "13",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060312",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/312",
abstract = "Studies have shown negative short-term effects of
early weaning (EW) in finfish larvae but information on
long-term effects of EW on growth and subsequent
economic loss is lacking. We evaluated the short- and
long-term effects of EW and late weaning (LW) on
Atlantic cod. Cod larvae were fed with enriched
rotifers from 2 to 35 days post-hatch (dph) and weaning
carried out from 21 to 35 dph (EW) or with enriched
rotifers from 2 to 29 dph followed by enriched Artemia
nauplii from 25 to 56 dph and weaning carried out from
45 to 56 dph (LW). At 190 dph, 50 fish from each tank
were tagged with an electronic tag and were transferred
to sea cages at 10 months old. At the end of 30 months
post-hatch, the weight of the fish was recorded. Our
results showed a significant short-term effect of the
weaning method on the growth of Atlantic cod at 65 dph,
but no significant difference at 90 and 190 dph.
However, fish from LW showed a significantly higher
body weight compared to fish from EW at 30 months
post-hatch. A cost analysis indicated substantial
benefit for commercial cod farming by using LW and we
recommend using LW to gain sizable financial benefit.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Eguiraun:2023:NLA,
author = "Harkaitz Eguiraun and Iciar Martinez",
title = "Non-Linear Analyses of Fish Behaviours in Response to
Aquatic Environmental Pollutants --- a Review",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "311",
day = "12",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060311",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/311",
abstract = "Analysis of fish behaviour is an effective way to
indirectly identify the presence of environmental
pollutants that negatively affect fish life, its
production and quality. Monitoring individual and
collective behaviours produces large amounts of
non-linear data that require tailor-suited
computational methods to interpret and manage the
information. Fractal dimension (FD) and entropy are two
groups of such non-linear analysing methods that serve
as indicators of the complexity (FD) and predictability
(entropy) of the behaviours. Since behavioural
complexity and predictability may be modulated by
contaminants, the changes in its FD and entropy values
have a clear potential to be embedded in a biological
early warning system (BEWS), which may be particularly
useful in Precision Fish Farming settings and to
monitor wild populations. This work presents a review
of the effects of a wide range of environmental
contaminants, including toxic compounds, cleaning and
disinfecting agents, stimulant (caffeine), anaesthetics
and antibiotics, heavy metals (lead, cupper, and
mercury), selenium, pesticides and persistent
environmental pollutants, on the FD and entropy values
of collective and individual behavioural responses of
different fish species. All the revised studies
demonstrate the usefulness of both FD and entropy to
indicate the presence of pollutants and underline the
need to consider early changes in the trend of the
evolution of their values prior to them becoming
significantly different from the control values, i.e.,
while it is still possible to identify the contaminant
and preserve the health and integrity of the fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:DDI,
author = "Yu-Jiao Li and Xin-Liang Wang and Ling-Yu Shi and
Zong-Yi Wang and Zi-Ang Zhao and Shu-Chao Ge and Bing
Hu",
title = "\gene{DUSP2} Deletion Inhibits Macrophage Migration by
Inhibiting \gene{ERK} Activation in Zebrafish",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "310",
day = "12",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060310",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/310",
abstract = "Dual-specificity phosphatase 2 (DUSP2) regulates the
activation of members of the mitogen-activated protein
kinase (MAPK) family, which is involved in a variety of
cellular processes including cell proliferation,
differentiation, apoptosis, and migration. DUSP2 also
regulates the expression of inflammatory mediators in
macrophages; however, it remains unknown whether DUSP2
participates in macrophage migration. Here, using the
tail fin injury model in zebrafish larvae, we found
that the deletion of DUSP2 inhibited the expression of
pro-inflammatory cytokines and macrophage chemokines.
Moreover, live imaging results showed that the
migration of macrophages to the injury site was
inhibited after DUSP2 deletion. This inhibitory effect
was mediated through the reduced activation of
extracellular regulated protein kinases (ERK) in DUSP2
knockout zebrafish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:TAR,
author = "Tong Wang and Yang Yang and Shirui Gong and Xi Wu and
Leilei Zeng and Yuhao Tao and Chaoyue Zhong and Leling
Song and Xiaochun Liu",
title = "Transcriptome Analysis Reveals Differences in Gene
Expression in the Muscle of the Brown-Marbled Grouper
(\bioname{Epinephelus fuscoguttatus}) with Different
Growth Rates",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "309",
day = "08",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060309",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/309",
abstract = "Brown-marbled grouper is one of the most important
mariculture species in China and is widely used as a
crossbreeding parent in the grouper industry. Enhancing
growth rates is a key target in fish breeding, and
gaining insight into the underlying mechanisms
responsible for growth differences between individuals
can aid in the improvement of grouper growth rates.
However, the mechanism behind growth differences in
this fish remains unclear. We analyzed the
transcriptome profiles of muscle tissues between fast-
and slow-growing brown-marbled grouper using RNA-seq
and identified 77 significantly up-regulated genes and
92 significantly down-regulated genes in the extreme
growth groups. Our findings suggest that up-regulated
genes such as ghr and tnni2, as well as down-regulated
genes such as stc2 and pdp1, are associated with growth
advantages in brown-marbled grouper. We used
differentially expressed genes (DEGs) for GO and KEGG
enrichment analyses. The results of the GO enrichments
showed that the significantly up-regulated genes in the
fast-growing group were involved in protein folding,
the actin cytoskeleton, the myosin complex, and other
processes. The results of the KEGG enrichments showed
that the significantly up-regulated genes in the
fast-growing group were involved in various pathways
such as glycolysis/gluconeogenesis, adipocytokine
signaling, MAPK signaling, carbon metabolism, and
PI3K-Akt signaling. Additionally, gene set enrichment
analysis (GSEA) showed that the PI3K/AKT/mTOR pathway
was up-regulated in the fast-growing group, which may
be responsible for the higher nutrient absorption
efficiency and muscle growth in these fish. Our results
contribute to a better understanding of the molecular
mechanisms and regulatory pathways underlying fast
growth in brown-marbled grouper. However, further
studies are necessary to fully elucidate the mechanisms
behind growth differences between individuals.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Segarra:2023:IRA,
author = "Sergi Segarra and Thanh Chau and Phuc Hoang and Loc
Tran",
title = "Immunoregulation and Resistance to Aquatic Pathogens
with Dietary Nucleotides in \geoname{Pacific} White
Shrimp, \bioname{Litopenaeus vannamei}",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "308",
day = "08",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060308",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/308",
abstract = "Using vegetable protein sources as a replacement for
fish meal (FM) in the diet of Pacific white shrimp
(PWS) has a negative impact on their health. Acute
hepatopancreatic necrosis disease (AHPND), caused by
Vibrio parahaemolyticus, affects PWS and causes
financial losses. Nucleotides modulate the immune
response and could contribute to counteracting these
issues. Our objective was to evaluate the effects of
nucleotide supplementation on performance, immune
response, and survival when challenged with V.
parahaemolyticus, in PWS receiving a diet with FM
partially replaced with vegetable protein sources. A
feeding trial (1000 PWS; 56 days) and a challenge trial
(600 PWS; 10 days) were performed using diets with
different FM inclusion levels (26\%, 23.4\%, 22.1\%,
and 20.8\%), with or without 0.1\% nucleotides. A
non-challenged, non-supplemented group was also used in
the challenge trial. Adding nucleotides to diets with
reduced FM allowed significantly better results in
growth performance parameters and total hemocyte count
(THC). In the challenge trial, compared to control,
nucleotide supplementation led to significantly higher
THC and survival rate 15 h post-challenge. In
conclusion, adding nucleotides to PWS diets improves
their immune response and resistance to aquatic
pathogens, allowing FM to be replaced by vegetable
protein sources without negatively affecting
performance.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Iskandar:2023:ECD,
author = "Nur Syuhada Iskandar and Noorashikin Md Noor and Zaidi
Che Cob and Simon Kumar Das",
title = "Elevated Carbon Dioxide and Its Impact on Growth,
Blood Properties, and Vertebral Column of Freshwater
Fish Mahseer, \bioname{Tor tambroides} Juveniles",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "307",
day = "07",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060307",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/307",
abstract = "The level of carbon dioxide, which resembles water
acidification, is critical to the success of juvenile
fish production. The growth, skeletal deformities, and
blood parameters of the economically important
freshwater fish mahseer, Tor tambroides, were assessed
in different concentrations of pCO 2 (400, 700, and
1000 {\SGMLmicro}atm pCO 2). The highest growth
properties (survival rate, body weight gain (BWG),
specific growth rate (SGR)) in mahseer were observed at
400 {\SGMLmicro}atm, whereas the lowest growth indices
were found in the treatment of 1000 {\SGMLmicro}atm pCO
2. The lowest pCO 2 concentration was found to be
within the optimum healthy blood parameter range. The
fish exposed to acidic conditions (700 and 1000
{\SGMLmicro}atm) exhibited considerably higher levels
of haemoglobin and haematocrit compared to the control
fish. Glucose levels were significantly lower in the
acidic conditions, while total cholesterol levels in
mahseer fish exposed to acidic conditions were higher.
The fish displayed skeleton malformations as the
concentration of pCO 2 was elevated. The findings from
this research could be set as a standard technique in
the juvenile rearing of mahseer fish under acidified
conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pereira:2023:SEC,
author = "Luciana A. Pereira and Leandro Castello and Donald J.
Orth and Fabrice Duponchelle and Eric M. Hallerman",
title = "A Synthesis of the Ecology and Conservation of
\bioname{Pseudoplatystoma} Catfishes in the
Neotropics",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "306",
day = "07",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060306",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/306",
abstract = "Species of the genus Pseudoplatystoma, the
long-whiskered catfishes, are important in commercial
and recreational fisheries in South America, and some
species have become key to regional aquaculture. Some
species of the genus are under pressure due to
overfishing and the negative impacts of dams. Six
questions are asked in this review: (i) What species
are in the genus, and where are they distributed? (ii)
What are the life histories and ecologies of
Pseudoplatystoma species? (iii) What are the patterns
of somatic growth for these species? (iv) What is known
about the biomass, production, and population dynamics
of Pseudoplatystoma? (v) What is the geographic
distribution of genetic variation within
Pseudoplatystoma species? (vi) What are the threats to
the conservation of these species? The taxonomy of the
genus currently includes eight species, respectively,
distributed over the Orinoco, Amazon, Paran{\'a}, and
S{\~a}o Francisco basins. Pseudoplatystoma catfishes
typically exhibit longitudinal migrations for
reproduction and lateral migration for feeding, but
these patterns may vary among populations. The size of
the first maturation of these catfishes varies between
57 cm to 82 cm in total length. Five of the eight
species spawn during the rising water season.
Pseudoplatystoma species can grow to about 130 cm in
total length and 100 Kg in weight and live until 30
years of age, depending upon the species. Biomass
production and population dynamics of these catfishes
have not yet been fully described. Their life-history
characteristics indicate that they are periodic
strategists with associated population recruitment
dynamics. Population genetic patterning varies among
Pseudoplatystoma species, with some degree of homing
behavior and genetic differentiation among populations,
indicating the need for management by applying the
Management Unit and perhaps Evolutionary Significant
Unit concepts. The main threats to the persistence of
these catfishes are overfishing and alterations in and
obstruction of river flow due to the construction of
hydropower dams. After synthesizing existing
information on species of the genus Pseudoplatystoma,
we offer suggestions for future research to fill
critical gaps in the knowledge of this group.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kruze:2023:PDI,
author = "Eriks Kruze and Andris Avotins and Loreta Rozenfelde
and Ivars Putnis and Ivo Sics and Laura Briekmane and
Jens Olsson",
title = "The Population Development of the Invasive Round Goby
\bioname{Neogobius melanostomus} in \geoname{Latvian
Waters} of the \geoname{Baltic Sea}",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "305",
day = "07",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060305",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/305",
abstract = "The invasive round goby (Neogobius melanostomus) was
established in the coastal waters of the Baltic Sea in
the early 1990s. The first observation of the species
in Latvian waters was in 2004. In the intervening
period, the population grew, the species became of
significance for local fisheries, and it likely
impacted the local ecosystem in the Baltic Sea. In this
study, we characterize the spatial--temporal population
development of round goby in Latvian coastal waters
using data from three different scientific and
fisheries-independent surveys. We also include data
from commercial fisheries landings to describe the
fisheries targeting the species. Our results suggest an
exponential increase in population numbers of round
goby in Latvian waters, peaking in 2018, followed by a
sharp decline. This observation is also supported by
data from commercial fisheries landings. We suggest
that intensive commercial fishing had a considerable
impact on the rapid decline of the species, but that
the decline was potentially amplified through a wider
scale decline, as observed in many areas of the Baltic
Sea. The results of this study contribute to the
knowledge base on the species and how fisheries can aid
in limiting the development of invasive fish
populations. Based on the results of the study, we also
provide recommendations for better future monitoring of
the species in the coastal waters of the Baltic Sea.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Quintanilla-Pineda:2023:PNP,
author = "Mario Quintanilla-Pineda and Jes{\'u}s D{\'\i}az and
Ana Guti{\'e}rrez-Falcon and Francisco C. Iba{\~n}ez
and Florencio Marzo",
title = "Profiling a New Postbiotic Product for Its Application
in Fish Aquaculture",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "304",
day = "06",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060304",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/304",
abstract = "Weissella cibaria is a lactic acid bacteria that has
been receiving great interest in the last decade;
however, its research into aquaculture remains
insufficient. In this context, the resistance to pH
3--5, bile salts (10--20\%), hydrophobicity, and
carbohydrate metabolisms of two W. cibaria strains
isolated from rainbow trout were evaluated. At the same
time, the postbiotic products of both W. cibaria were
produced under different conditions, and their
stability to heat (95--130 {$^\circ $}C) and
proteolytic enzymes were determined. The low pH
sensitivity of two pathogens (Aeromonas salmonicida
subsp. salmonicida and Yersinia ruckeri) was also
recorded. Both strains of W. cibaria survived pH and
bile salt treatments. Neither expressed enzymatic
activity or hydrophobic characteristics with the
executed methods. Treatment with heat, enzymes, and
transfer to vegetal broth significantly reduced (p
{$<$} 0.05) the postbiotic product's antibacterial
activity. On the other hand, no significant difference
(p {$<$} 0.05) was reported in antibacterial
characteristics when postbiotics were produced by
coculturing both strains. Low pH does not significantly
influence pathogen growth (p {$<$} 0.05). Our findings
suggest that the studied strains do not adjust as
probiotics, but the postbiotic product obtained from
them could become a promising tool as a supplement food
for fish aquaculture applications.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Molina:2023:HTS,
author = "Alfredo Molina and Phillip Dettleff and Valentina
Valenzuela-Mu{\~n}oz and Cristian Gallardo-Escarate and
Juan Antonio Vald{\'e}s",
title = "High-Temperature Stress Induces Autophagy in Rainbow
Trout Skeletal Muscle",
journal = j-FISHES,
volume = "8",
number = "6",
pages = "303",
day = "06",
month = jun,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8060303",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:43 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/6/303",
abstract = "Ectothermic animals, such as teleosts, have
increasingly been exposed to stressful high-temperature
events due to global warming. Currently, the effects of
thermal stress on skeletal muscle, a key tissue for
fish growth, are unknown. This study examined the
impact of high-temperature stress on the skeletal
muscle transcriptome of rainbow trout (Oncorhynchus
mykiss) in control (15 {$^\circ $}C) and
high-temperature (20 {$^\circ $}C) conditions.
Additionally, we examined the plasmatic levels of
cortisol, glucose, and creatine kinase activity, and
examined oxidative damage and autophagy activation in
skeletal muscle. High-temperature stress induced
significant increases in cortisol and glucose plasmatic
levels. Nevertheless, no changes were observed in
creatine kinase activity in plasma and skeletal muscle
oxidation. Skeletal muscle RNA was isolated and
sequenced using the HiSeq Illumina platform. A total of
383,796,290 reads were mapped onto the reference
rainbow trout genome. The transcriptomic analysis
showed that 293 genes were upregulated in the
high-temperature group, mainly associated with
autophagosome assembly, amino acid transport, and the
glutamine metabolic process. On the other hand, 119
genes were downregulated in the high-temperature group,
mainly associated with digestion, proteolysis, and the
muscle contraction process. In addition, RT-qPCR of
differentially expressed representative genes and
Western blot analysis of LC3-II/LC3-I levels confirmed
skeletal muscle autophagy induced by high temperature.
This study sheds light on intriguing facets of the
adaptive response of rainbow trout skeletal muscle to
high-temperature stress and provides significant
insights into the physiology of autophagy in
teleosts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ferreira:2023:CLL,
author = "In{\^e}s Ferreira and Felippe A. Daros and Cl{\'a}udia
Moreira and Diana Feij{\'o} and Alberto Rocha and Ana
Mendez-Vicente and Jorge Pisonero and Alberto Teodorico
Correia",
title = "Is \bioname{Chelidonichthys lucerna} ({Linnaeus},
1758) a Marine Estuarine-Dependent Fish? {Insights}
from Saccular Otolith Microchemistry",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "383",
day = "24",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070383",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/383",
abstract = "Tub gurnard, Chelidonichthys lucerna (Linnaeus, 1758),
is a Mediterranean-Atlantic benthic species usually
captured as by-catch by Portuguese traditional
fisheries and considered the most important commercial
fish species of the family Triglidae. However, to date,
little is known about its habitat residency and whether
the species can be considered a marine
estuarine-dependent fish. Otolith microchemistry has
proven effective in providing information about fish
movement patterns throughout different water
salinities. In this study, core-to-edge transects of
Sr:Ca in the fish saccular otoliths of 35 juveniles of
C. lucerna captured in March 2007 by a scientific
survey along the Portuguese coast were used to assess
the species movement between brackish and marine
waters. Data suggest that most individuals (97\%) have
an estuarine-dependent profile, with 63\% showing a
clear presence in marine waters during the early life
history periods. Evidence of an estuarine residence
throughout the fish life cycle was found in only 3\% of
individuals. Ba:Ca profiles did not reflect an inverse
relationship with Sr:Ca salinity results but
corroborated findings from other authors about the
influence of upwelling processes and freshwater runoffs
on Ba incorporation into the otoliths of coastal fish.
Furthermore, the data also showed that C. lucerna can
occupy and migrate among habitats with diverse salinity
degrees, showing high environmental plasticity and
adaptation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vehanen:2023:PIC,
author = "Teppo Vehanen and Tapio Sutela and Ari Huusko",
title = "Potential Impact of Climate Change on Salmonid Smolt
Ecology",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "382",
day = "24",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070382",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/382",
abstract = "The migratory life history of anadromous salmonids
requires successful migration between nursery, feeding,
and spawning habitats. Smolting is the major
transformation anadromous salmonids undergo before
migration to feeding areas. It prepares juvenile fish
for downstream migration and their entry to seawater.
We reviewed the effects of climate change on smolt
ecology from the growth of juveniles in fresh water to
early post-smolts in the sea to identify the potential
effects of climate change on migratory salmonid
populations during this period in their life history.
The focus was especially on Atlantic salmon. The shift
in suitable thermal conditions caused by climate change
results in Atlantic salmon expanding their range
northward, while at the southern edge of their
distribution, populations struggle with high
temperatures and occasional droughts. Climatic
conditions, particularly warmer temperatures, affect
growth during the freshwater river phase. Better growth
in northern latitudes leads to earlier smolting.
Thermal refuges, the areas of cooler water in the
river, are important for salmonids impacted by climate
change. Restoring and maintaining connectivity and a
suitably diverse mosaic habitat in rivers are important
for survival and growth throughout the range. The start
of the smolt migration has shifted earlier as a
response to rising water temperatures, which has led to
concerns about a mismatch with optimal conditions for
post-smolts in the sea, decreasing their survival. A
wide smolt window allowing all migrating phenotypes
from early to late migrants' safe access to the sea is
important in changing environmental conditions. This is
also true for regulated rivers, where flow regulation
practices cause selection pressures on migrating
salmonid phenotypes. The freshwater life history also
affects marine survival, and better collaboration
across life stages and habitats is necessary among
researchers and managers to boost smolt production in
rivers. Proactive measures are recommended against
population declines, including sustainable land use in
the catchment, maintaining a diverse mosaic of habitats
for salmonids, restoring flow and connectivity, and
conserving key habitats.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Baudoin:2023:AAV,
author = "Brooke A. Baudoin and Bonnie L. Brown and Robin D.
Calfee and Jill A. Jenkins",
title = "Amino Acid Variation at the Mitochondrial Binding Site
of {Antimycin A} is Proposed to Reflect Sensitivity and
Toxicity Differences among Fish Species",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "381",
day = "22",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070381",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/381",
abstract = "To better understand differential sensitivities among
fish species to the piscicidal compound Antimycin-A
(ANT-A), we hypothesized that variations in amino acids
at the ANT-A binding site may reflect toxicity
differences. Protein sequences for six motifs
comprising the ANT-A binding site were obtained and
compared for invasive carp species (N = 515) and seven
non-target species (N = 277); a consensus was
delineated from each species. The carp species, Common
Carp (Cyprinus carpio), Silver Carp (Hypophthalmichthys
molitrix), Bighead Carp (Hypophthalmichthys nobilis),
Grass Carp (Ctenopharyngodon idella), and Black Carp
(Mylopharyngodon piceus), showed the same amino acids
at the site; thus, it was termed the carp consensus
motif sequence (CCM). Channel Catfish (Ictalurus
punctatus) showed the most amino acid polymorphisms,
with three motifs 96--100\% different from CCM. Within
a species, Bluegill (Lepomis macrochirus) and Fathead
Minnow (Pimephales promelas) variation per motif was
most dissimilar (46.7\% and 21.6\%, respectively).
Organismal mortality data from the literature indicated
Yellow Perch (Perca flavescens), Walleye (Sander
vitreus), and American Gizzard Shad (Dorosoma
cepedianum) to be most sensitive to the piscicide,
Catfish least sensitive, and all others intermediate.
The protein sequence variations of the binding site
appeared to be in accord with organismal sensitivity
categories when they differed from the CCM; the motifs
in Gizzard Shad and Walleye were the same as in CCM.
The physical/chemical nature of ANT-A is important to
consider in organismal response comparisons. This
cellular approach of studying ANT-A binding at its
target enzyme is a non-destructive way to predict
piscicidal efficacy of ANT-A against fishes of
interest, informs management decisions in control
efforts for invasives, and can be used to forecast
effects on sympatric species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sibiya:2023:BPG,
author = "Ashokkumar Sibiya and Khalid A. Al-Ghanim and
Marimuthu Govindarajan and Marcello Nicoletti and
Nadezhda Sachivkina and Baskaralingam Vaseeharan",
title = "Biochemical Patterns and Genotoxicity of the Endocrine
Disruptor Metformin in the Freshwater Fish
\bioname{Labeo rohita}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "380",
day = "22",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070380",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/380",
abstract = "Metformin is one of the most extensively used drugs,
making it one of the most likely endocrine disruptors
in the environment, which may negatively affect fish
and other freshwater animals. Still, there is a dearth
of studies examining metformin's impact on freshwater
creatures, like fish. This research aimed to identify
the biochemical and genotoxicity effects of the
endocrine disruptor metformin in the freshwater fish
Labeo rohita at ecologically appropriate doses.
Metformin's toxicity was evaluated by subjecting L.
rohita to the drug over 28 days at two dosages (40
{\SGMLmicro}g/L to 80 {\SGMLmicro}g/L). The results
indicated that 40 {\SGMLmicro}g/L and 80
{\SGMLmicro}g/L of metformin caused an increase in
reactive oxygen species and the generation of free
radicals in the body of L. rohita, which in turn caused
impairment and alterations in total hemoglobin, red
blood corpuscles, white blood corpuscles, oxidative
stress, lipid peroxidation, protein carbonyl activity,
respiratory burst activity, myeloperoxidase activity,
and lysozyme activity. In addition, animals treated
with the maximum metformin dose (80 {\SGMLmicro}g/L)
demonstrated substantial DNA damage in the genotoxicity
experiment. Metformin's endocrine-disrupting actions
may have unintended ramifications for the well-being of
aquatic species in their natural habitats. Results of
the study demonstrated a serious concern that metformin
exposure might be harmful to aquatic life.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2023:CBE,
author = "Jinming Wu and Jinping Wu and Huan Ye and Wei Xiong
and Wanmin Qu and Xiaoqian Leng and Hao Du",
title = "Correlations between Environmental Factors and the
Distribution of Juvenile \bioname{Hucho bleekeri} in
the \geoname{Taibai River, Shaanxi, China}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "379",
day = "21",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070379",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/379",
abstract = "Sichuan taimen (Hucho bleekeri) is a national highly
protected wild animal that faces significant impacts
from habitat degradation and loss. To study the habitat
selection by H. bleekeri, data on the Taibai River and
the distribution of juvenile H. bleekeri were recorded
seasonally between February 2017 and January 2018, and
habitat selection was assessed using the suitability
curve method. The results indicate that the average
distribution density of juvenile H. bleekeri in the
Taibai River is 0.08 {\pm} 0.09 ind./m, with an optimal
elevation range from 1200 to 1600 m, river sinuosity
range from 1.0 to 1.2, and vegetation coverage between
0.7 and 1.0. When choosing a microhabitat, juvenile H.
bleekeri individuals tend to inhabit water areas with a
water depth of 0.65 {\pm} 0.33 m, flow velocity of 0.50
{\pm} 0.24 m/s, and offshore distance of 7.66 {\pm}
4.25 m. Furthermore, smaller juvenile fish prefer
nearshore habitats with lower flow velocities and
shallower water depths. The results provide technical
support for the protection and restoration of the
habitat of H. bleekeri.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yuan:2023:MCE,
author = "Shuya Yuan and Zhaosheng Sun and Qian Gao and Zhen Li
and Zhitao Qi and Sidi Zheng and Danjie Liu",
title = "Molecular Characterization and Expression Analysis of
\gene{NLRC3}-like, {ASC}, and \gene{Caspase1} in
Spotted Sea Bass (\bioname{Lateolabrax maculatus})",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "378",
day = "21",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070378",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/378",
abstract = "Nucleotide-binding oligomerization domain (NOD)-like
receptor (NLR) family members are innate immune sensors
involved in the recognition of highly conserved
pathogen-associated molecular patterns (PAMPs).
Apoptosis-associated speck-like protein (ASC) is a
critical adaptor molecule in multiple inflammasome
protein complexes, mediating inflammation and host
defense. Caspase1, an inflammatory caspase, has been
documented to play important roles in the innate immune
system. In this study, we identified and characterized
NLRC3-like, ASC, and Caspase1 (referred to as Lm
NLRC3L, Lm ASC, and Lm Caspase1) from the spotted sea
bass (Lateolabrax maculatus). A sequence analysis
revealed that Lm NLRC3L, Lm ASC, and Lm Caspase1 shared
similar features with their fish counterparts. Lm
NLRC3L contained a FISNA domain, a NACHT domain, and
four LRR motifs, followed by a C-terminal fish-specific
B30.2 domain. Lm ASC possessed a PYRIN domain for
interacting with inflammasome sensor proteins, as well
as a CARD domain. Lm Caspase1 had a CARD domain at its
N-terminus and a CASC domain at its C-terminus. These
three genes were ubiquitously distributed in the liver,
spleen, head kidney, gill, intestine, skin, muscle, and
brain. They share similar expression patterns, and all
demonstrate the highest level of expression in the
gill. We analyzed the expression changes in genes in
the spleen, gill, and head kidney after stimulation
experiments in vivo. After lipopolysaccharide (LPS)
stimulation, the expression levels of these three genes
were significantly upregulated in the short term,
followed by significant downregulation at 48 and 72 h
in some examined tissues. Following Edwardsiella tarda
infection, these three genes were upregulated in
various tissues. However, the expressions of these
three genes were not affected by
polyinosinic-polycytidylic acid (poly (I:C))
stimulation. Overall, our results indicate that these
three genes are involved in the immune response against
bacterial infection in the spotted sea bass, providing
the foothold for understanding the immune function and
mechanism of the fish inflammasome.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:AIA,
author = "Yadong Zhang and Abubakar Shitu and Shengyu Hang and
Zhangying Ye and Wen Xu and Hangfang Zhao and Jian Zhao
and Songming Zhu",
title = "Assessing the Impacts of Aquaculture Soundscapes on
the Growth, Physiology and Behavior of
\bioname{Micropterus salmoides}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "377",
day = "21",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070377",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/377",
abstract = "Sound has a potential impact on animal welfare and
production, but the impacts of soundscapes on
aquaculture species in different aquaculture production
systems have been rarely studied. This study
investigated the impact of varying aquaculture
soundscapes on largemouth bass (Micropterus salmoides).
Three soundscapes were administered to replicated
tanks: Recirculating Aquaculture System (RAS:107.7 dB
re 1 Pa RMS), In-Pond Raceway System (IPRS:115.1 dB re
1 Pa RMS), and Ambient (70.4 dB re 1 Pa RMS) as the
control. The initial weight of fish in the three groups
was 3.59 {\pm} 0.30 g. Following a 50-day experimental
period, the average weight of the Ambient group (14.08
{\pm} 0.13 g) was significantly greater than that of
the IPRS group (12.79 {\pm} 0.08 g) (p {$<$} 0.05).
Examination of physiological samples revealed that the
soundscape negatively impacted the fish's
immunological, anti-oxidation, and digestive enzymes.
Furthermore, the external noise also influenced the
locomotive patterns of the fish aggregations. Fish
polarity and cohesion were significantly more discrete
(p {$<$} 0.05) in both the RAS (47.79 {\pm}
{2.34$^\circ $} and 98.52 {\pm} 3.22 mm) and IPRS
groups (48.04 {\pm} {0.70$^\circ $} and 87.70 {\pm}
7.31 mm) compared to the Ambient group (42.76 {\pm}
{1.42$^\circ $} and 85.73 {\pm} 1.57 mm). These results
highlight the significant impacts of the aquaculture
soundscape on the development, physiological
activities, and behavioral traits of largemouth bass.
Future research should focus on determining and
optimizing the impact of different equipment noise to
ensure optimal welfare and production performance in
aquaculture systems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:RTL,
author = "Jiaming Wang and Xiangbo Yin and Guodong Li",
title = "A Real-Time Lightweight Detection Algorithm for Deck
Crew and the Use of Fishing Nets Based on Improved
{YOLOv5s} Network",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "376",
day = "20",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070376",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/376",
abstract = "A real-time monitoring system for the operational
status of fishing vessels is an essential element for
the modernization of the fishing industry. The
operational status of fishing vessels can be identified
by using onboard cameras to detect the deck crew and
the use of fishing nets. Due to the typically limited
processing capacity of shipboard equipment and the
significant memory consumption of detection models,
general target detection models are unable to perform
real-time image detection to identify the operational
status of fishing vessels. In this paper, we propose a
lightweight real-time deck crew and the use of a
fishing net detection method, YOLOv5s-SGC. It is based
on the YOLOv5s model, which uses surveillance cameras
to obtain video of fishing vessels operating at sea and
enhances the dataset. YOLOv5s-SGC replaces the backbone
of YOLOv5s with ShuffleNetV2, replaces the feature
fusion network with a modified Generalized-FPN, and
adds the CBAM attention module in front of the
detection head.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esposito:2023:MCS,
author = "Ana{\"\i}s Esposito and Jean-Jos{\'e} Filippi and
Charlotte Gerbaud and Quentin Godeaux and R{\'e}mi
Millot and Paul-Jean Agostini and Camille Albertini and
Eric Durieux and Jos{\'e}phine Foata and Yann
Quilichini",
title = "Macroparasite Communities with Special Attention to
Invasive Helminths in \geoname{European} Eels
\bioname{Anguilla anguilla} from Freshwaters and
Brackish Lagoons of a \geoname{Mediterranean} Island",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "375",
day = "20",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070375",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/375",
abstract = "An extensive survey of macroparasites in 320 European
eel Anguilla anguilla (Linnaeus, 1758) was conducted in
two brackish lagoons and eleven freshwater localities
in the Mediterranean island of Corsica (France) between
spring 2021 and winter 2021--2022. It resulted in the
identification of nineteen parasites: two Monogea, four
Digenea, one Copepoda, four Acanthocephala, three
Cestoda, and five Nematoda, including the first
geographical records, as Corsican freshwater sites were
studied for the first time. The silvering stage was
determined, and the eels were aged through
otolithometry to compare parasite communities. Classic
parasitology indices, a multivariate analysis, and an
analysis of indicator values (IndVal) showed clear
preferences towards the host's habitat and salinity.
Seasonal variations were shown for several parasites. A
dataset from the same two coastal lagoons was used to
study the changes in the parasite communities over the
last decade, and this showed an increase in the
prevalence and abundance of three invasive helminth
species: the Monogenea Pseudodactylogyrus bini
(Kikuchi, 1929), Pseudodactylogyrus anguillae (Yin and
Sproston, 1948) Gusev, 1965 and the Nematoda
Anguillicola crassus Kuwahara, Niimi, and Itagaki,
1974. These pathogenic parasites were found in all
sampled localities, except for the two Monogenea in the
polyhaline-to-euhaline Urbino lagoon. It is thus
advised that future management measures take into
account the environmental preferences of the most
concerning parasites.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{deAguiar:2023:EDP,
author = "Gustavo Augusto Carvalho Costacurta de Aguiar and
Cristiana Leonor da Silva Carneiro and Daniel Abreu
Vasconcelos Campelo and Rafael Costa Teixeira Rusth and
Jo{\~a}o Felipe Ribeiro Maciel and Bernardo
Baldisserotto and Jener Alexandre Sampaio Zuanon and
Alexmiliano Vogel de Oliveira and Maria Goreti de
Almeida Oliveira and Mariella Bontempo Duca de Freitas
and Wilson Massamitu Furuya and Ana L{\'u}cia Salaro",
title = "Effects of Dietary Peppermint (\bioname{Mentha
piperita}) Essential Oil on Growth Performance, Plasma
Biochemistry, Digestive Enzyme Activity, and Oxidative
Stress Responses in Juvenile \geoname{Nile} Tilapia
(\bioname{Oreochromis niloticus})",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "374",
day = "20",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070374",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/374",
abstract = "This study aimed to evaluate the supplementation of
peppermint essential oil (Mentha piperita; PEO) in
diets for juvenile Nile tilapia (Oreochromis
niloticus). A feeding experiment with diets containing
graded levels (0.0, 0.2, 0.4, 0.6, 0.8, and 1.0 g kg
{$-$1}) of PEO was carried out with fish of 0.58 {\pm}
0.08 g. The inclusion of graded levels of PEO in the
diet improved the weight gain (0.52 g kg {$-$1}), feed
intake (0.51 g kg {$-$1}), and feed conversion (0.51 g
kg {$-$1}) of juvenile Nile tilapia in a quadratic
pattern. Increasing levels of PEO also led to a linear
decrease in body lipid content. The gut activity of the
digestive enzymes amylase (0.54 g kg {$-$1}) and
protease (0.39 g kg {$-$1}) increased quadratically,
whereas lipase activity increased linearly. PEO
increased the activity of the antioxidant enzymes
catalase (CAT; 0.52 g kg {$-$1}) and superoxide
dismutase (SOD; 0.58 g kg {$-$1}) while reducing the
production of malonaldehyde (MDA; 0.40 g kg {$-$1}) and
nitric oxide (NO; 0.63 g kg {$-$1}) in the liver. The
results of this study provide evidence of the
beneficial effects of PEO on the growth and health of
Nile tilapia and recommend a dose of 0.6 g kg {$-$1} as
the optimal level of supplementation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Adachi:2023:GPS,
author = "Aisni M. C. L. Adachi and Pollyana C. G. Roque and
Fabio H. V. Hazin and Marcelo Vianna and Matheus M.
Rotundo and Claudio Oliveira and Fausto Foresti and
Vanessa P. Cruz",
title = "Genetic Population Structure and Diversity of the
Whitetail Dogfish \bioname{Squalus albicaudus}
({Chondrichthyes, Squaliformes}) along the
\geoname{Brazilian Coast} as Identified by {SNP}
Markers",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "373",
day = "20",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070373",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/373",
abstract = "The shark Squalus albicaudus, categorized by the
International Union for Conservation of Nature red list
as Data Deficient due to lack of minimal information
for classification, is distributed throughout the
Brazilian coast. High pressures such as overfishing and
anthropic activities, as well as certain biological
characteristics, including k strategists, comprise
influential shark stocks reduction agents. However,
genetic diversity, population structure, connectivity,
and effective population size data are still limited
for S. albicaudus, indicating the need for further
studies. In this context, the genetic variability and
population structure of S. albicaudus were investigated
herein to test for panmixia. Samples were obtained from
coasts of the Brazilian states of Pernambuco, Rio de
Janeiro, and S{\~a}o Paulo along the species
distribution range, and single nucleotide polymorphisms
(SNPs) were assessed by the ddRADseq method. The
findings revealed a panmitic S. albicaudus population,
explained by certain life strategies, such as polyandry
and migratory behavior. Based on the genomic findings
reported herein, a single S. albicaudus population
should be considered in the study area, indicating the
need for specific management and conservation plans at
the regional scale.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2023:CPC,
author = "Yingru Wu and Qi Du and Yueqin Liao and Shanshan Shui
and Jie Pang and Soottawat Benjakul and Bin Zhang",
title = "Changes in Physicochemical Characteristics and
Volatile Flavor Compounds of Brine-Preserved
Ready-to-Eat Shrimp (\bioname{Solenocera crassicornis})
during Chilled Storage",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "372",
day = "19",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070372",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/372",
abstract = "This study aimed to explore the changes in the quality
of ready-to-eat peeled shrimp (Solenocera crassicornis)
during chilled storage. The cooked shrimp were soaked
in hermetically sealed jars, which were treated by
three soaking methods: distilled water; 4\% NaCl
solution; and 4\% NaCl, 0.34\% citric acid, 4.23\%
trehalose, and 0.04\% VC solution (defined as the
control, experimental 1 (E1), and experimental 2 (E2)
groups, respectively). The shelf-life of the E2 group
was 20 days longer than that of the control group.
Sensory scores, color, and textural results confirmed
the E2 group exhibited better sensory scores and color,
and the degradation of the physical structure of shrimp
muscle was delayed during chilled storage. In addition,
the total volatile basic nitrogen (TVB-N), total viable
count (TVC), and thiobarbituric acid reactive
substances (TBARS) of shrimp muscle in each group all
showed an increasing trend, but these values were
significantly lower in the E2 group than those in the
control and E1 groups during chilled storage. The
results of gas chromatography--ion mobility
spectrometry (GC--IMS) and principal components
analysis (PCA) showed that the volatile organic
compounds (VOCs) in the three groups of RTE shrimp
muscle changed, but small changes in VOCs were observed
in E2 during chilled storage. These results provide new
ideas and references for peeled shrimp product
development and quality assurance technology.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Furtado:2023:HCL,
author = "Maura da Silva Costa Furtado and Joaquim Carlos
Barbosa Queiroz and Bianca Bentes and Edson Koiti Kudo
Yasojima and D{\'e}bora de Oliveira Thomaz and Larissa
da Costa Pinheiro and Mauro Luis Ruffino and Victoria
Isaac",
title = "The Hydrological Cycle of the \geoname{Lower Amazon in
Brazil} Determines the Variation in Local Fishing
Patterns",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "371",
day = "19",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070371",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/371",
abstract = "Fishery catches on the Lower Amazon River were
analyzed in relation to the hydrological cycle,
temporal shifts in fishing effort, and the use of nets
or lines in lakes and rivers. The study was based on a
temporal series of catch data collected between January
1993 and June 2011. The variables analyzed were the
total catch, use of gillnets or lines, the environment
targeted (lakes or rivers), and fishing effort.
Temporal trends in the data series were analyzed using
the Mann--Kendall test. An Analysis of Covariance
(ANCOVA) was used to evaluate whether gillnet and line
catches had independent effects on the catch data.
Duncan's test was applied to identify the groups
(months) of means that were significantly different
from each other. The majority of the catch landings
were taken with gillnets (98.65\%) in a lake
environment (64.98\%). The temporal series showed a
significant decline over time in the gillnet catches
from both lakes and rivers, as well as in fishing
effort. The influence of the annual flood cycle was
reflected in the catches and the flood pulse regulated
fishing productivity patterns in the region. The
fishers who adapt their activities to this flood pulse
have good traditional knowledge of the environment. The
results of the study also indicated that any changes in
this dynamic system may impact traditional local
fisheries and affect the economic wellbeing of local
fisher populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Guo:2023:IEC,
author = "Ruijie Guo and Kai Yu and Kai Huang and Qiang Lin and
Ting Liu",
title = "Immunoprotective Effect of \bioname{Coptis
chinensis}-Supplemented Diet on \bioname{Streptococcus
agalactiae} Infection in Tilapia",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "370",
day = "19",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070370",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/370",
abstract = "This study aimed to investigate the immunoprotective
effect of Coptis chinensis (CC) on Streptococcus
agalactiae (SA) infection in tilapia. Experimental fish
were randomly divided into two groups feeding on a
normal diet (ND) and a CC-supplemented diet (CCD) for 2
weeks and then injected with SA. After the inoculation
experiment, the ND and CCD groups were named PI\_ND and
PI\_CCD, respectively. CCD increased superoxide
dismutase (SOD) activity and decreased malondialdehyde
(MDA) activity significantly before and after
infection. Immunological assays revealed that the serum
interleukin-1{\beta} (IL-1{\beta}), complement 3 (C3),
immunoglobulin M (IgM), Interferon-gamma
(IFN-{\gamma}), and tumor necrosis factor-{\alpha}
(TNF-{\alpha}) levels in the CCD group were
significantly higher than in the ND group both before
and after infection. In addition, proteomics analysis
of liver tissue identified 62 differentially expressed
proteins (DEPs) in CCD vs. ND, and 36 DEPs in the
PI_CCD vs. the PI_ND groups. Furthermore, 80 specific
upregulated proteins and 49 specific downregulated
proteins were screened in the CCD group. The specific
upregulated proteins included important antimicrobial
enzymes such as lysozymes and cathepsin D, and
antimicrobial peptides such as septins, granulin, and
grancalcin, involving multiple KEGG brite categories
such as enzymes, exosomes, membrane transport, and
proteolipid proteins. Furthermore, specific
downregulated proteins were enriched in
glycolysis/gluconeogenesis and TCA cycle pathways. In
conclusion, CC supplementation effectively enhances the
ability of tilapia to resist SA infection by modulating
various antioxidant enzymes, immune factors,
antimicrobial enzymes, and antimicrobial peptides, and
by moderately inhibiting central carbon metabolism.
These findings provide a basis for replacing
antibiotics with environmentally-friendly functional
aquatic feeds to control bacterial diseases.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:HHD,
author = "Xinghao Li and Xueli Huang and Liya Zhao and Wei Cai
and Yuhe Yu and Jin Zhang",
title = "Host Habitat as a Dominant Role in Shaping the Gut
Microbiota of Wild Crucian Carp (\bioname{Carassius
auratus})",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "369",
day = "17",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070369",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/369",
abstract = "Current knowledge on the fish gut microbiota has
largely been obtained from experiments on
laboratory-reared animals. Here, the crucian carp
(Carassius auratus) with a mean weight of 159.9 {\pm}
11.4 g (mean {\pm} SD) were collected from their
natural habitats (i.e., Wuhu lake and Poyang lake,
China), and the gut microbiota were analysed by using
the next-generation sequencing of 16S rRNA gene. We
obtained more than 430,000 high-quality reads, which
constituted more than 1200 operational taxonomy units
(OTUs), revealing extremely diverse microbes in the
fish gut. Proteobacteria, Fusobacteria, Bacteroidetes
and Firmicutes were detected as the prominent phyla
(each {$>$} 1\% of total abundance) within the gut
microbiota, regardless of the host habitat or the gut
segment (i.e., foregut vs. hindgut). Although the
microbes in the hindgut were more diverse (OTU number,
Shannon and Chao1; One-way Anova, p {$>$} 0.05) than in
the foregut, the host habitat had a significant role in
shaping the community structures (MRPP, ANOSIM,
PERMANOVA, p {$<$} 0.01). Interestingly, we also
detected a set of common OTUs, whereby genera Aeromonas
and Cetobacterium might comprise the core gut
microbiota of crucian carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:CII,
author = "Xiubao Chen and Hongbo Liu and Karsten Liber and Tao
Jiang and Jian Yang",
title = "Copper-Induced Ionoregulatory Disturbance,
Histopathology, and Transcriptome Responses in
Freshwater Mussel (\bioname{Anodonta woodiana}) Gills",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "368",
day = "14",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070368",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/368",
abstract = "Copper (Cu) contamination has become a severe problem
in freshwater environments worldwide. The freshwater
mussel Anodonta woodiana is used as a unique
bioindicator to monitor Cu contamination in freshwater
environments. However, Cu toxicity and response
mechanisms in A. woodiana are still largely unknown. A
sublethal acute exposure experiment (2.0 mg/L Cu
exposure for 72 h) was conducted to investigate the
effects of Cu bioaccumulation on ionoregulatory
homeostasis, histological features, and transcriptome
responses using A. woodiana gills as indicator tissue.
The gill bioaccumulation capacity was up to 474. Cu
bioaccumulation decreased Na + and Mg 2+ concentrations
(p {$<$} 0.05) by 82\% and 17\%, respectively, and
induced cilia loss, epithelial desquamation, and
filament atrophy of the gills. Transcriptome analysis
identified 3160 differentially expressed genes (DEGs),
including 1870 upregulated and 1290 downregulated
genes. GO enrichment analysis showed that cellular
processes, metabolic processes, biological regulation,
and responses to stimuli contained the most DEGs in the
biological processes. KEGG pathway analysis showed that
apoptosis, arginine and proline metabolism, the
toll-like receptor signaling pathway,
apoptosis-multiple species, histidine metabolism,
beta-alanine metabolism, cytokine--cytokine receptor
interaction, and the p53 signaling pathway were
significantly enriched. These findings provide
comprehensive evidence for exploring Cu toxicity and
response mechanisms in freshwater mussels.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Weber:2023:EII,
author = "Gregory M. Weber",
title = "Effects of {IGF1} and {IGF2} on In Vitro Ovarian
Follicle Maturation in Rainbow Trout,
\bioname{Oncorhynchus mykiss}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "367",
day = "14",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070367",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/367",
abstract = "Ovarian follicle maturation (OFM), including the
resumption of meiosis in the oocyte, is under hormonal
regulation. Insulin-like growth factors (IGF) have been
shown to participate in the regulation of OFM with
species-specific actions in teleost fishes. In the
present study, in vitro treatment of rainbow trout
ovarian follicles with recombinant human insulin-like
growth factor-1 (rhIGF1) or rhIGF2 did not induce
germinal vesicle breakdown (GVBD), a marker for the
resumption of meiosis, in the oocytes. Co-incubation of
follicle-enclosed oocytes with rhIGF1 and the
maturation-inducing steroid (MIS) in rainbow trout,
17{\alpha},20{\beta}-dihydroxy-4-pregnen-3-one
(17,20{\beta}P), also did not induce GVBD in follicles
from rainbow trout that were not able to respond to the
MIS alone suggesting IGFs cannot induce oocyte
maturational competence (OMC), which is the ability of
the oocyte to respond to the MIS. Nevertheless, the
addition of rhIGF1 with the MIS increased the
proportion of oocytes completing GVBD compared with MIS
alone, although this potentiation was small and varied
greatly among clutches of follicles from fish with
oocytes at different stages of germinal vesicle
migration. Collectively, these observations suggest
IGFs may have synergistic actions with the MIS but
cannot induce resumption of meiosis directly at the
oocyte and are not potent inducers of OMC in rainbow
trout. Rainbow trout are the first teleost fish in
which IGFs were found to induce neither OMC nor
resumption of meiosis in vitro.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shija:2023:EBP,
author = "Vicent Michael Shija and Kwaku Amoah and Jia Cai",
title = "Effect of Bacillus Probiotics on the Immunological
Responses of \geoname{Nile} Tilapia
(\bioname{Oreochromis niloticus}): a Review",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "366",
day = "13",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070366",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/366",
abstract = "The use of antibiotics and therapeutics for Nile
tilapia has increased along with its increased
production and intensification. Probiotics have been
studied as an alternative to the excessive use of
antibiotics in fish diets. Studies have demonstrated
that dietary Bacillus probiotic inclusion promotes
faster growth and disease resistance in farmed fish
species. Despite advances in research, there is still
uncertainty about the mechanisms controlling the immune
response of fish. In this review, we summarize and
discuss recent research on the immune responses of Nile
tilapia to Bacillus probiotics. Through the use of
immunological information including phagocytosis,
lysozyme activity, respiratory activity, antioxidant
proteins, complement activity, and immune-related gene
expression, researchers hope to develop effective
strategies for successfully using Bacillus probiotics
in Oreochromis niloticus farming.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bourquin:2023:CGC,
author = "Rebecca Bourquin and Michael J. Moore and Donald J.
Orth and Eric M. Hallerman",
title = "Conservation Genetics of Clinch Dace
\bioname{Chrosomus {\rm sp. cf.} saylori}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "365",
day = "13",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070365",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/365",
abstract = "Clinch Dace (Chrosomus sp. cf. saylori) is a newly
recognized and yet-undescribed species of minnow with a
restricted and fragmented distribution in the upper
Tennessee River basin in southwestern Virginia, USA. We
collected Clinch Dace from seven streams and observed
variations at nine selectively neutral microsatellite
DNA loci to infer population genetic processes and
identify units for conservation management. Bayesian
cluster analysis showed that three of the seven
surveyed populations were genetically distinct, while
the other four populations showed signs of recent
admixture. Estimated effective population sizes and m
-ratios were low within most populations, suggesting
loss of alleles due to recent genetic drift. Positive F
IS values, high average individual inbreeding
coefficients, and high degrees of inferred relatedness
among individuals suggested that inbreeding is taking
place in some populations. F ST values were high, and
analysis of molecular variance indicated genetic
divergence among populations. These indicators suggest
that Clinch Dace populations are subject to the genetic
processes that are characteristic of small and isolated
populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chiu:2023:CRB,
author = "Pei-Sheng Chiu and Shine-Wei Ho and Cheng-Hsuan Huang
and Yen-Chun Lee and Yu-Hung Lin",
title = "Captive Reproductive Behavior, Spawning, and Early
Development of White-Barred Goby \geoname{Amblygobius
phalaena} ({Valenciennes}, 1837) and Examined Larval
Survival and Viability at Different Water Temperatures
and Salinities",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "364",
day = "12",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070364",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/364",
abstract = "White-barred goby Amblygobius phalaena is a highly
valued marine ornamental fish, but its captive
reproduction and early life history are poorly
understood. In this study, the captive reproductive
behavior, early development, and optimal temperature
and salinity for the larval survival and viability of
A. phalaena were investigated for the first time.
Spawning occurred between 11:00 and 13:00, with the
breeding pairs naturally spawning 24 times from 1 June
2021 to 30 June 2022. The fecundity ranged from 11,022
to 95,858 eggs per spawning event. Hatching occurred
approximately 81 h and 26 min after fertilization at a
temperature of 27.0 {\pm} 0.9 {$^\circ $}C. Newly
hatched larvae had a total length (TL) of 1.91--2.03 mm
with 24--26 somites. The larvae transformed into
juveniles at 30 days post-hatch. Experiments were
conducted at different temperatures (21, 24, 27, 30,
and 33 {$^\circ $}C) and salinities (18, 24, 30, and 36
ppt) to determine the optimal conditions for larval
survival and viability. The results indicate that the
most suitable conditions in terms of temperature were
in the range of 21--27 {$^\circ $}C and 30 ppt for
salinity. These findings provide valuable insights for
the future development of captive-breeding techniques
and the commercial production of other marine
ornamental gobies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Montag:2023:FRS,
author = "Luciano Foga{\c{c}}a de Assis Montag and Luiz
Ant{\^o}nio Wanderley Peixoto and Lidia Brasil Seabra
and Liziane Amaral Barbosa Gon{\c{c}}alves and Cleonice
Maria Cardoso Lobato and Marina Barreira Mendon{\c{c}}a
and Tiago Octavio Begot and Erival Gon{\c{c}}alves
Prata and Tiago Magalh{\~a}es da Silva Freitas",
title = "First Record of Spinal Deformity in the \geoname{South
American} Silver Croaker \bioname{Plagioscion
squamosissimus} ({Eupercaria: Sciaenidae}) in the
\geoname{Xingu River, Brazil}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "363",
day = "12",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070363",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/363",
abstract = "Observations of skeletal malformations in fish in the
wild are poorly documented and need to be investigated.
Here we report the occurrence of body shortening in
specimens of Plagioscion squamosissimus collected in
the Volta Grande do Xingu, middle Xingu River region
(Par{\'a}, Brazil), during a 12-month monitoring
program (2021--2022). We observed morphological
anomalies in nine individuals, of which two underwent
radiographic analysis, recording the fusion and
compression of vertebrae in different portions of the
spine. The average percentage decrease in body length
resulting from the deformity was 23.8\%. This is the
first record of malformation in this species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2023:EBP,
author = "Xiaojuan Hu and Yu Xu and Haochang Su and Wujie Xu and
Guoliang Wen and Chuangwen Xu and Keng Yang and Song
Zhang and Yucheng Cao",
title = "Effect of a Bacillus Probiotic Compound on
\bioname{Penaeus vannamei} Survival, Water Quality, and
Microbial Communities",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "362",
day = "11",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070362",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/362",
abstract = "Given the widespread use of commercial probiotics in
aquaculture, it is important to evaluate the quality
and environmental effects of these additives. Here, the
effects of a Bacillus probiotic compound, BG4, on
Penaeus vannamei survival rate, water quality factors,
and microbial communities were assessed. An analysis of
the BG4 powder confirmed the content of probiotic
bacteria: 50.2\% Bacillus licheniformis, 48.4\%
Bacillus subtilis, and 1.4\% Bacillus
amyloliquefaciens, consistent with the information on
the product packaging. The effective bacterial quantity
(1.1 $ \times $ 10 9 CFU/g) was higher than that
indicated on the product label (2 $ \times $ 10 8
CFU/g). BG4 was added to a sterilized aquaculture water
system, according to the indicated bacterial quantity;
after 7 days, the maximum ammonia nitrogen, phosphate,
and chemical oxygen demand degradation rates were
36.3\%, 28.9\%, and 15.2\%, respectively. In the shrimp
culture experiment, the survival rate of P. vannamei
and water quality did not differ significantly between
the BG4 and control groups. The bacterial quantity and
high-throughput sequencing results indicated that
Bacillus content in BG4 group decreased continuously
from 3.5 $ \times $ 10 4 CFU/mL to 6.9 $ \times $ 10 2
CFU/mL. To ensure the desired effect of probiotics in
commercial aquaculture applications, additional quality
evaluations and scientific assessments are needed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Andriamahefazafy:2023:GDW,
author = "Mialy Andriamahefazafy",
title = "Governing Distant-Water Fishing within the Blue
Economy in \geoname{Madagascar}: Policy Frameworks,
Challenges and Pathways",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "361",
day = "11",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070361",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/361",
abstract = "Madagascar's vast oceanic space hosts distant-water
fishing (DWF) fleets from Taiwan, Japan, South Korea,
Spain, France and others since the 1960s, making DWF a
substantial component of the blue economy. Considering
this extensive experience of managing DWF activities
for more than 60 years, this paper explores the
existing policy frameworks and challenges regarding
managing DWF. The results show while it is well
equipped legally, the country is struggling to
implement its national policies and laws while
continuing to adopt new management frameworks. This is
due to a limited coherence on long-term policy making
and policy implementation, resulting in a mismatch
between the two, and a paradoxical vision that promotes
DWF without the means to monitor fishing activities and
their impacts. The existing institutional settings and
governance frameworks make change possible in
Madagascar's approach to DWF. To improve the management
of DWF, this paper outlines four pathways. These
include (i) a greater attention on the implementation,
harmonisation and evaluation of existing policies and
projects; (ii) continuing efforts on transparency for
DWF operations and contract negotiations; (iii)
realigning aspirations and policies with local needs;
and (iv) taking better advantage of measures adopted at
the regional Indian Ocean level to improve national
management of DWF. An efficient implementation of an
improved policy and legal framework could contribute to
strengthening the governance of DWF activities toward
sustaining national benefits while preserving coastal
livelihoods.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mao:2023:IAS,
author = "Hanping Mao and Bin Wang and Jian Zhao and Yafei Wang
and Xiaoxue Du and Qiang Shi",
title = "Influences of Aquaponics System on Growth Performance,
Antioxidant Parameters, Stress Parameters and Gene
Expression of \bioname{Carassius auratus}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "360",
day = "11",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070360",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/360",
abstract = "Aquaponics is a new type of composite farming system,
which combines aquaculture and hydroponics through
ecological design to achieve scientific synergism.
However, the effects of aquaponics on the welfare
status and stress parameters of fish are unclear. In
this study, 150 crucian carp with an average initial
body weight of 7.06 {\pm} 0.32 g were selected. Nine
fish were randomly selected as the control group (NC
group and hypoxia group) for stress, antioxidant and
gene expression parameters after acclimation and
hypoxia stress, respectively. The remaining crucian
carps were randomly divided into three experimental
groups with 20 crucian carps in each group (T 0, T 1
and T 2, respectively), with three replicates. The
fresh weight of the lettuce root in the T 2 group had
no significant effect (p {$>$} 0.05). Compared with the
control group (T 0 and T 1), there were significant
differences in the specific growth rate, the weight
gain rate, the fresh leaf weight, the chlorophyll
content and the water quality parameters in group T 2
(p {$<$} 0.05). Regarding the biochemical parameters,
superoxide dismutase and catalase showed significant
differences between the T 2 and T 1 groups (p {$<$}
0.05). At the same time, the HSP70 and Prdx3 genes were
upregulated in the liver of the T 2 group compared to
the NC group and the hypoxia group. The research
suggests that aquaponics may reduce the hypoxia stress
of crucian carp without affecting the growth of crucian
carp and lettuce.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Peng:2023:HAG,
author = "Zhilan Peng and Xiaomin Liu and Ming Jin and Yu Zhan
and Xiaolin Zhang and Yongbo Bao and Minhai Liu",
title = "Hypoxia Activates \gene{HIF-1$ \alpha $} and Affects
Gene Expression and Transcriptional Regulation of {PHD}
in \bioname{Tegillarca granosa}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "359",
day = "11",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070359",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/359",
abstract = "Hypoxia is a common challenge faced by mollusks, and
the role of hypoxia-inducible factor-1{\alpha}
(HIF-1{\alpha}) in regulating related target genes
under hypoxia in Tegillarca granosa (Tg) remains
unclear. In this study, we identified gene
HIF-1{\alpha} and further explored its function.
qRT-PCR was performed to determine the mRNA expression
of HIF-1{\alpha}, prolyl 4-hydroxylases (PHD),
hemoglobin (Hb), and myoglobin (Mb) in response to
hypoxia. Dual-luciferase reporter analysis was used to
assess the transcriptional activity of HIF-1{\alpha} on
the PHD and Hb genes during hypoxia. Results showed
that the expression levels of HIF-1{\alpha} and PHD
mRNAs were highest in the gill and lowest in the
adductor muscle. Under hypoxic conditions,
HIF-1{\alpha} and PHD mRNAs were both induced, and
their expression levels increased significantly,
peaking at 8 h and gradually decreasing thereafter. The
transcription of Tg-PHD was induced by hypoxia and was
Tg-HIF-1{\alpha} dependent. Notably, the expression of
Hb decreased in hemocytes (p {$<$} 0.01) after 8 h at
0.5 mg/L, while the expression of MbI and MbII
increased (p {$<$} 0.01) in the hepatopancreas after 24
h and 120 h, respectively. Moreover, Tg-HIF-1{\alpha}
could transactivate the PHD promoter but not that of
Hb. These findings provide valuable insights into the
regulatory role of Tg-HIF-1{\alpha} on Tg-PHD,
safeguarding it from degradation by PHD and offering
significant contributions to the understanding of
HIF-1{\alpha}'s function. Nonetheless, the expression
of Tg-HIF-1{\alpha} protein was not detected under
hypoxic conditions, and additional investigations are
required to quantify Tg-HIF-1{\alpha} protein dynamics
and establish the correlation between mRNA expression
and protein levels.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2023:TAR,
author = "Ren-Xie Wu and Yan-Shan Liang and Su-Fang Niu and Jing
Zhang and Bao-Gui Tang and Zhen-Bang Liang",
title = "Transcriptomic Analysis Reveals Circadian Rhythm
Homeostasis in Pearl Gentian Grouper under Acute
Hypoxia",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "358",
day = "10",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070358",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/358",
abstract = "Oxygen level is an important environmental factor
affecting the circadian rhythm. However, little is
known about the molecular mechanism by which clock
genes regulate the circadian rhythm in fish under
hypoxia. To explore changes in the transcription and
expression of clock genes and related molecular
regulatory mechanisms in pearl gentian grouper under
hypoxia, liver transcriptome data were analyzed after
exposure to acute hypoxic stress (dissolved oxygen 0.5
mg/L) for 1, 3, 6, and 9 h. miR-210 and m0044-5p
inhibited the expression of period3 (per3) and casein
kinase 1 delta b (csnk1db) in the core loop of the
circadian clock, respectively. The nuclear receptor
subfamily 1 group d member 1 (nr1d1) and RAR-related
orphan receptor b (rorb) genes in the auxiliary loop
were jointly up-regulated by three miRNAs
(miR-144-3p/5p, miR-361-5p, and miR-133) and the
transcription factor nuclear receptor subfamily 1 group
d member 2 (Nr1d2). The pearl gentian grouper maintains
the stability of circadian clock systems and normal
physiological metabolism under hypoxic stress by
regulating the transcriptional expression of these
genes via miRNAs and transcription factors to improve
hypoxic tolerance. These findings provide important
basic data for future research on hypoxic tolerance in
pearl gentian grouper and provide new insights into the
interaction between hypoxia and the circadian rhythm in
fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Elbahnaswy:2023:BCI,
author = "Samia Elbahnaswy and Gehad E. Elshopakey and Medhat S.
Shakweer and Elsayed A. A. Eldessouki and Abdelwahab A.
Abdelwarith and Elsayed M. Younis and Simon J. Davies
and Mai A. M. El-Son",
title = "Bacterial Co-Infection as a Potential Threat to Farmed
Flathead Grey Mullet (\bioname{Mugil cephalus}):
Phenotypic and Molecular Diagnosis, Histopathology,
Immunity Response, and In Vitro Antibacterial
Evaluation",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "357",
day = "10",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070357",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/357",
abstract = "Vibrio alginolyticus, Vibrio chlorea, and Enterococcus
cloacae were the main bacterial pathogens investigated
during recurrent episodes of mortalities among farmed
flathead grey mullet (Mugil cephalus) in the Bahar
El-Baqar area, Sharqia governorate, Egypt, during the
summer season in 2021. Nevertheless, the contamination
of water with heavy metals was evaluated. In this
study, a total number of sixty moribund and freshly
dead Mugil cephalus were examined clinically and upon
post-mortem. Then, the bacterial isolates were
subjected to bacteriological phenotypic examination,
16S rRNA gene sequencing, and antibiotic sensitivity
testing. Histopathological screening and the immunity
response of naturally infected M. cephalus were also
implemented. In areas where bacterial strains were
recovered in a high percentage, water contamination
with Cu, Fe, and Ni was higher than the permissible
limit. The results showed a high similarity (99--100\%)
to Vibrio and E. cloacae strains using phylogenetic
analysis of 16S rRNA. Most isolates exhibited
sensitivity to florfenicol, ciprofloxacin, and
norfloxacin, while they were resistant to erythromycin
and amoxicillin. Hemorrhage, oedema, hemosiderosis, and
melanomacrophage activation in most affected tissues of
infected M. cephalus were detected by light microscopy.
Nevertheless, higher levels of AST and ALT, as well as
serum inflammatory markers, CRP, and lysozyme activity,
were shown in infected fish. There were higher MDA
levels, along with a reduction of GSH, SOD, and
catalase, in the gills and brain of infected M.
cephalus, compared to the control fish. Our obtained
data confirmed the high prevalence of Vibrio serovars
and E. cloacae in infected grey mullets, describing
their histopathological alterations, higher
inflammatory response, and oxidative stress impact.
These results could be useful indicators for
understanding the pathogenesis of these pathogens in
correlation with water parameter deterioration. Thus,
the emergence of Vibrio and E. cloacae species in this
study threatens the brackish water aquaculture industry
in Egypt and poses a public health concern.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2023:TBA,
author = "Tangjian Zhou and Chaofeng Jia and Qian Meng and
Dafeng Xu and Zhiwei Zhang and Fei Zhu and Yonglei Zhao
and Ruijian Sun and Yunxia Yang and Shuyin Chen",
title = "Transcriptome-Based Analysis of the Liver Response
Mechanism of Black Porgy (\bioname{Acanthopagrus
schlegelii}) to Stocking Density",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "356",
day = "08",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070356",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/356",
abstract = "Long-term high stocking density often brings negative
effects such as decreased body weight, decreased
immunity, and increased mortality to cultured fish,
while the effects of short-term stocking are relatively
less studied. In this experiment, we characterized
spatial and temporal gene expression in black porgy
(Acanthopagrus schlegelii) liver by establishing two
stocking density groups --- high (H: 26.5 kg/m 3) and
low (L: 2.95 kg/m 3) --- and conducting transcriptome
sequencing before collecting liver samples at two time
points: 6 h (AL and AH) and 60 d (CL and CH) into the
culture trial. There were 648 and 550 differentially
expressed genes (DEGs) in the AL-vs-AH and CL-vs-CH
groups, respectively. The DEGs in the AL-vs-AH group
were significantly enriched in steroid biosynthesis,
terpenoid backbone biosynthesis, the PPAR signaling
pathway, proteasome, aminoacyl-tRNA biosynthesis, and
ribosome biogenesis in eukaryotes. The DEGs in the
CL-vs-CH group were more significantly enriched in
lipid metabolism-related pathways, such as the PPAR
signaling pathway, fatty acid metabolism, and
cholesterol metabolism. In addition, immune-related
pathways such as the phagosome and complement and
coagulation cascades were also enriched in the CL-vs-CH
group. The expression changes concerning the DEGs in
each group were further analyzed. The DEGs related to
steroid synthesis, protein synthesis, and the
degradation pathways were down-regulated, but
immune-related genes were generally up-regulated in the
AH group. The DEGs related to lipid synthesis were
significantly up-regulated in the CH group, but the
DEGs related to lipid consumption and utilization were
down-regulated. The expression of immune-related DEGs
was also negatively affected in the CH group. These
results suggest that acute and chronic crowding stress
affects lipid metabolism and immune regulation in the
liver of black porgy. Acute stress particularly
affected hepatic protein metabolism, while chronic
stress showed more effects on hepatic lipid metabolism.
The results of this study provide a theoretical basis
for understanding the effects of high stocking
densities upon black porgy and may also provide a
reference for crowding stress regulation studies in
other fish species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Banaduc:2023:DDA,
author = "Doru B{\u{a}}n{\u{a}}duc and Sergey Afanasyev and John
Robert Akeroyd and Aurel N{\u{a}}stase and Ion
N{\u{a}}vodaru and Lucica Tofan and Angela
Curtean-B{\u{a}}n{\u{a}}duc",
title = "The \geoname{Danube Delta}: The {Achilles} Heel of
\geoname{Danube River--Danube Delta--Black Sea} Region
Fish Diversity under a \geoname{Black Sea} Impact
Scenario Due to Sea Level Rise --- a Prospective
Review",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "355",
day = "07",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070355",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/355",
abstract = "The Danube Delta is one of Earth's biodiversity
hotspots and includes many endemic, rare, and important
species of both major conservation and economic value.
This unique complex of ecosystems also plays a key role
for Danube River and Black Sea fish fauna through its
role as a natural safe buffer, shelter, feeding,
reproduction, and smooth transitional area for a large
number of fish species. Climate change is inducing a
progressive sea level rise in the Black Sea, a fact
that is expected to impact the delta's key complex and
dynamic habitats, biocoenoses, and associated biota,
and last but not least the key taxonomic group, namely,
fish. Around one-third of the fish species of this
delta will be greatly affected, sometimes negatively,
by this climate change scenario, another one-third to a
lesser extent, and the final one-third not at all. The
ecological positive feedback of fish can stimulate
environmental change and is expected to be responsible
for changes within Danube Delta ecosystems, and also
for the near Danube River and Black Sea diverse matrix
of aquatic and semi-aquatic ecosystems. Sea level rise
in the Black Sea is considered to have been one of the
main stress factors of the Danube Delta fish fauna in
the past, and is likely to be the case in the future.
In this spatio-temporal dynamic context, for the fish
species under threat and risk, in situ-adapted
management measures are highly required. The current
work brings for the first time such a prospective
knowledge about the potential impact on Danube
River--Danube Delta--Black Sea coast fish diversity in
the potential climate change--sea level rise
scenario.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2023:EEP,
author = "Guo Hu and Feng Chen and Ying Zhang and Peixian Luan
and Zhiyuan Luo and Jiangong Niu and Peng Zheng and Sai
Wang and Tao Zhang and Yongjun Shu and Feng Ji",
title = "Estimates of the Effective Population Size and Genetic
Structure of the Critically Endangered Ship Sturgeon
(\bioname{Acipenser nudiventris}) in the
\geoname{Chinese} Section of the \geoname{Ili River}",
journal = j-FISHES,
volume = "8",
number = "7",
pages = "354",
day = "07",
month = jul,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8070354",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:44 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/7/354",
abstract = "Ship sturgeon (Acipenser nudiventris) is a critically
endangered fish that is listed on the International
Union for Conservation of Nature's Red List of
Threatened Species. Sixteen individuals from the
Chinese section of the Ili River were genotyped using
genome re-sequencing technology. By applying a genomic
relatedness estimation with 1,527,694 genome-wide SNP
markers, we found that the coancestry coefficients
showed a high level of relatedness between individuals.
The effective population sizes over 500 generations
were estimated, and this showed that the effective
population sizes began to dramatically decline from
about 14,840 to 171 individuals when going back four
generations from the current population. Artificial
reproduction techniques guided by genomic relatedness
may be a valuable approach to the conservation of this
critically endangered fish species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huang:2023:PGB,
author = "Lishi Huang and Guosong Zhang and Yupeng Zhang and
Xinlan Li and Zhong Luo and Wenyu Liu and Fu Luo and
Haifeng Liu and Shaowu Yin and Jun Jiang and Xia Liang
and Quanquan Cao",
title = "Profiling Genetic Breeding Progress in {Bagrid}
Catfishes",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "426",
day = "21",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080426",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/426",
abstract = "The Bagridae fish family holds significant economic
value and enjoys a high demand in the commercial market
due to its desirable characteristics, such as delicious
meat, fewer muscle spines, and a pleasing flavor. In
recent years, the Bagridae has experienced rapid
development in the seed industry in response to
industry demands. Researchers have conducted extensive
studies on genetic breeding in the Bagridae, employing
a comprehensive breeding approach that combines
conventional breeding techniques with innovative new
technologies and cooperative efforts. In this review,
we present an overview of the market situation and
discuss various breeding processes applied to the
Bagridae, including selective breeding, genetic
engineering breeding, cell engineering breeding,
molecular marker-assisted breeding, and hybrid
breeding. Additionally, we focus on introducing the
cultivation methods for two new Bagridae varieties.
Furthermore, we explore and summarize the breeding
conditions, genetic background, heterosis,
comprehensive breeding technology, stress resistance,
and metabolic nutrition of hybrid Bagridae catfishes.
Overall, noticeable heterosis has been observed in the
breeding of the hybrid yellow catfish ``Huangyou-1'',
which exhibits promising market prospects and economic
benefits for aquaculture. Our study aims to underscore
the importance of harnessing the heterosis of catfish,
particularly the Pelteobagrus and Bagridae species.
Consequently, this review provides an update on the
current available information regarding the seed
industry of the Bagridae fish family.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:MCR,
author = "Zewen Zhang and Xiaohui Li and Guiwei Zou and Hongwei
Liang",
title = "Molecular Characterization and Response of Silver Carp
(\bioname{Hypophthalmichthys molitrix}) {GLUT1} under
Hypoxia Stress",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "425",
day = "20",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080425",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/425",
abstract = "As an important freshwater species with economic and
ecological benefits, silver carp (Hypophthalmichthys
molitrix) exhibits poor tolerance to hypoxia. Glucose
transporters (GLUTs) are core membrane proteins that
transport glucose to tissues and regulate essential
life activities. Its expression is regulated by
HIF-1{\alpha} and cells in hypoxic conditions to
maintain energy demand through GLUTs inducing enhanced
glucose transport. We cloned H. molitrix glut1 (SLC2A1)
and analyzed its sequence using bioinformatics tools.
The glut1 cDNA was 2104 base pairs long and encoded a
490 amino acid protein. Phylogenetic analysis revealed
that sliver carp glut1 is evolutionarily conserved and
exhibited the highest sequence similarity with
Ctenopharyngodon idella glut1. Glut1 expression was the
highest and lowest in the gills and liver,
respectively. Hypoxic stress significantly increased
glut1 expression in the brain (p {$<$} 0.05); in the
gills, it was the highest and lowest in the
semi-asphyxia and asphyxia groups, respectively; in the
liver, it was significantly higher under hypoxia than
that of the normoxia group; and in the heart, it was
significantly higher in the floating head,
semi-asphyxia, and asphyxia groups than in the normoxia
group (p {$<$} 0.05). The proposed mechanism may thus
provide the basis for elucidating the molecular basis
of silver carp's hypoxia stress response mediated by
glut1.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sava:2023:PPM,
author = "Alexandru Sava and Paul Uiuiu and C{\u{a}}lin
La{\c{t}}iu and Daniel Cocan and
George-C{\u{a}}t{\u{a}}lin Muntean and Tudor Papuc and
Andrada Ihu{\c{t}} and Camelia R{\u{a}}ducu and Anca
Becze and Cristina Craioveanu and Camelia Munteanu and
Radu Constantinescu and Vioara Mire{\c{s}}an",
title = "{PAHs}, Physicochemical and Microbiological Analyses
of Trout Processed by Traditional Smoking, in Different
Types of Packaging",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "424",
day = "19",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080424",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/424",
abstract = "Smoking represents one of the oldest thermic
processing methods of fish, and it was originally used
to preserve fish for later meals, delaying spoiling.
The objective of this study was to analyze the
physicochemical and microbiological safety and quality
of two traditionally smoked trout species (Oncorhynchus
mykiss and Salvelinus fontinalis). We analyzed the
effect of trout processed by traditional smoking,
characterizing the samples according to the
relationships existing between classical microbial
analysis, physicochemical parameters, and the content
of polycyclic aromatic hydrocarbons (PAHs). The
microbial activity of the smoked fish was very low.
Although traditional smoking implies high temperatures
and direct exposure to the smoke, Benzo[a]pyrene,
Benzo[b]fluoranthene, Benzo[k]fluoranthene,
Dibenzo[a,h]anthracene, Benzo[g,h,i]perylene, and
Indeno [1,2,3-cd]pyrene compounds had undetectable
levels in the samples. The products obtained in this
study did not exceed the maximum values imposed by EU
legislation regarding the TVB-N values during the 30
days of the experiment. We conclude that traditionally
smoked trout has many advantages in terms of quality
and safety of the products. Thus, the obtained products
are safe for consumption for at least 30 days after
processing.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Uyisenga:2023:ERF,
author = "Adolphe Uyisenga and Hualiang Liang and Mingchun Ren
and Dongyu Huang and Chunyu Xue and Heng Yin and
Haifeng Mi",
title = "The Effects of Replacing Fish Meal with Enzymatic
Soybean Meal on the Growth Performance, Whole-Body
Composition, and Health of Juvenile {Gibel} Carp
(\bioname{Carassius auratus gibelio})",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "423",
day = "18",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080423",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/423",
abstract = "Fish meal (FM) constitutes the main, expensive
component in aquatic diets. However, the supply of FM
is no longer sufficient to sustain global aquaculture
production. This study had the primary goal of
assessing if the replacement of FM with enzymatic
soybean meal (ESBM) can affect the performance of
growth and immunological response in juvenile Gibel
carp. Juvenile fish with an initial weight of 45.02
{\pm} 0.03 g were arbitrarily assigned to 18 fish cages
of 1 m 3 each, then fed with diets of different levels
of ESBM (0\% (control group), 4\%, 8\%, 12\%, 16\%, and
20\%) for 159 days. These diets corresponded,
respectively, to the replacement levels of 0\% (control
group), 20\%, 40\%, 60\%, 80\%, and 100\% FM by ESBM.
For the parameters of growth and whole-body
composition, no obvious differences were found between
the control group and other replacement levels (p {$>$}
0.05). Similarly, none of the replacement levels showed
significant effects for alanine transaminase (ALT),
total cholesterol (TC), alkaline phosphatase (ALP), and
glucose (GLU) levels (p {$>$} 0.05). Malondialdehyde
(MDA) levels, as well as the activities of superoxide
dismutase (SOD) and total antioxidant capacity (T-AOC)
in plasma, were not significantly affected at all
replacement levels, according to the findings of this
study (p {$>$} 0.05). The replacement level of 60\%
significantly increased the activities of catalase
(CAT), whereas the replacement levels of 20\% and 100\%
markedly decreased the activities of this enzyme (p
\SGMLentity{706} 0.05). Hepatic and intestinal tissues
in this study did not show obvious alterations at all
levels of replacement.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hwang:2023:PFC,
author = "Ju-Ae Hwang and Jun Seong Park and Hae Seung Jeong and
Hyeongsu Kim and Seung-Yoon Oh",
title = "Productivity of Fish and Crop Growth and
Characteristics of Bacterial Communities in the
{FLOCponics} System",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "422",
day = "18",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080422",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/422",
abstract = "Aquaponics (AP) and biofloc technology (BFT) systems
rely heavily on bacterial communities to break down
organic matter and cycle nutrients that are essential
for fish and plant growth. The functional roles of
bacterial communities in aquaculture systems are
critical to their sustainable operation. Currently, the
research on the combination of BFT and AP systems
called FLOCponics (FP) is lacking, thereby hindering
our ability to optimize their performance. Here,
several characteristics (productivity of fish and
crops, physicochemical properties of water, and
bacterial community) in FP systems cultivating Japanese
eel (Anguilla japonica) and leaf lettuce Caipira
(Lactuca sativa) were compared to those in the BFT
system. Additionally, the effect of fish density on the
FP system was investigated. The results indicated that
the FP system was more productive than the BFT system.
Fish growth rate was highest in the FP system (52.6\%),
and the average body weight of eels was 168.2 {\pm}
26.8 g in the FP system compared to 140.3 {\pm} 27.0 g
in the control (BFT, 5 kg/m 2). However, increasing
fish density resulted in lower growth rates, with a
growth rate of 20.6\% observed in the high-density (20
kg/m 2) experimental group. The bacterial composition
was also significantly different between the systems
and fish densities, suggesting that bacterial
communities may be closely related to the performance
of the aquaponics system.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2023:SDF,
author = "Jiahao Liu and Feiran Zhang and Qiang Ma and Yuliang
Wei and Mengqing Liang and Houguo Xu",
title = "Sex Differences in Fatty Acid Composition of
\geoname{Chinese} Tongue Sole (\bioname{Cynoglossus
semilaevis}) Tissues",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "421",
day = "17",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080421",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/421",
abstract = "The fatty acid accumulation in fish tissues are
closely related to fish sex, yet the sex differences in
fish fatty acids have not been fully known. This
experiment investigated the sex differences in fatty
acid composition of Chinese tongue sole (Cynoglossus
semilaevis), which have typical sexual dimorphism and
reversal. Three sexes, namely females, males and
pseudomales, were involved in this study. Five batches
of fish samples were collected from different fish
farms in Shandong Province, China. These fish were
identified to be at the gonadal development stage of
III--IV. The disparity of fatty acid between sexes
(female vs male and pseudomale vs. male) was evaluated
by the distance coefficient (D sex) and multi-variate
similarity of percentages analysis (SIMPER). The
highest D sex values between females and males were
found in gonad, followed by liver and eye. The fatty
acids that differed between females and males were
mainly DHA, 16:1n-7, 18:1n-9, and 18:0. The sex
difference in DHA and 18:1n-9 contributed significantly
to the overall sex difference in fatty acid of brain,
eye, and gonad, whereas that in 16:1n-7 contributed
significantly in heart, gill, liver, and muscle. The
sex difference in 18:0 contributed significantly to the
overall sex difference in fatty acid of heart, gill,
gut, and skin. The sex differences in fatty acid
between pseudomales and males were generally
insignificant. In conclusion, at this gonadal
development stage (III--IV), the sex differences in
fatty acid between female and male Chinese tongue sole
have emerged, mainly in the gonad. The testes had
higher DHA levels but lower levels of 16:1n-7 and
18:1n-9 than ovaries. In addition, for pseudomales, the
phenotypic sex more substantially determined the fatty
acid composition than the genetic sex.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garces:2023:ABT,
author = "Sara Garc{\'e}s and Gabriele Lara",
title = "Applying Biofloc Technology in the Culture of
\bioname{Mugil cephalus} in Subtropical Conditions:
Effects on Water Quality and Growth Parameters",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "420",
day = "17",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080420",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/420",
abstract = "Mugil cephalus is an estuarine species that has been
shown to acclimate to a diversity of environmental
conditions being a good candidate to diversify
aquaculture. The objective of this study was to
evaluate the growth and water quality of M. cephalus
juveniles in a biofloc technology system (BFT). Over a
period of 87 days, mullet juveniles (117.36 {\pm} 6.48
g) were reared in two experimental conditions: water
exchange (control group) (50\% three times a week) and
BFT (C:N ratio of 15:1). M. cephalus juveniles were
stocked at a density of 7.2 kg{\cdot}m {$-$3}. Water
quality parameters were monitored periodically. Fish
were fed with a balanced artificial feed for marine
fish (2\% of the total biomass). The water quality
parameters were similar between the treatments, with
the exception of pH, alkalinity, TSS, and N-NO 3 (p
{$<$} 0.05). Zootechnical parameters WG, DWG, BG, SGR,
condition factor, and survival did not show significant
differences (p {$>$} 0.05). The biomass gain was higher
in BFT (p {$<$} 0.05). Water quality variables did not
appear to influence mullet survival under culture
conditions. Mullet is a species that can be reared in
BFT without compromising productive performance and
having a significant saving of water.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lin:2023:EPY,
author = "Hao Lin and Sishun Zhou and Zhangfan Huang and
Jianrong Ma and Lumin Kong and Yi Lin and Zhongying
Long and Huihui Qin and Longhui Liu and Yanbo Zhao and
Zhongbao Li",
title = "The Effects of \bioname{Porphyra yezoensis}
Polysaccharides on Intestinal Health of Spotted Sea
Bass, \bioname{Lateolabrax maculatus}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "419",
day = "15",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080419",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/419",
abstract = "Porphyra yezoensis polysaccharides (PPs) have
biological activities such as promoting digestion,
functioning as antioxidants, and improving intestinal
health. The aim of this study was to investigate the
effects of PPs on the intestinal health of spotted sea
bass (Lateolabrax maculatus). A total of 360 spotted
sea bass (10.53 {\pm} 0.02 g) were randomly divided
into six groups. Fish in each group were fed with
varying PP concentrations (0, 3, 6, 9, 12, 15 g/kg) for
52 days. The results showed that 12 g/kg PPs
significantly increased the body weight gain, specific
growth rate, and condition factor, while there was no
significant change in the feed conversion ratio. A
supplementation dosage of 9 g/kg PPs significantly
increased intestinal amylase activity, and 12 g/kg PPs
supplementation significantly increased intestinal
trypsin activity. In addition, compared with the
control group, there was no difference in lipase in the
experimental groups. When fed 9 g/kg, the intestinal
glutathione content was significantly increased, and
the malondialdehyde content was significantly
decreased; the effect of PPs on the catalase activity
was not significant. PPs improved intestinal
morphology, specifically by improving the intestinal
villus morphology and increasing the intestinal lining
surface area. Compared with the control group, PPs
increased the abundance of Firmicutes and Bacteroides.
At the genus level, Cyanobacteria, Muribaculaceae, and
Lachnospiraceae were the main flora in the intestinal
tract of spotted sea bass. In addition, PPs increased
ACE and Chao1 indices of the intestinal microorganisms,
while the Simpson index and Shannon index did not
change significantly, indicating that intestinal
microbial composition and abundance had increased to a
certain extent. The results indicated that different
levels of PPs in feed can improve the intestinal
physiological and biochemical indexes, intestinal
morphology, and microbial composition, and thus improve
the intestinal health of spotted sea bass.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Totoiu:2023:ETS,
author = "Aurelia {\c{T}}o{\c{t}}oiu and Neculai Patriche and
Victor Ni{\c{t}}{\u{a}} and Elena S{\^\i}rbu and
Floricel Maricel Dima and Magda Ioana Nenciu and Veta
Nistor",
title = "Epidemiology of Turbot (\bioname{Scophthalmus
maeoticus}) Bacterial Contamination, a Fishery Limiting
Factor on the \geoname{Romanian Black Sea}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "418",
day = "15",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080418",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/418",
abstract = "The aim of this research was to evaluate the health
status of the Scophthalmus maeoticus (Pallas, 1814)
population from the Romanian marine area in 2016--2019,
by identifying and investigating information about
bacterial and constitutional diseases, establishing the
influence of these bacterioses on the researched
populations, and highlighting the main biological
disorders (reproduction, growth, and feeding) that
manifested in the analyzed fish. The bacterial diseases
reported in S. maeoticus (Pallas, 1814) populations
were caused by pathogens from the genera Vibrio,
Aeromonas, and Pseudomonas. Numerous skin diseases,
especially various types of ``wounds'', lesions of bony
tubercles, and tissue lesions may be the subsequent
cause of neoplasms, as a result of fishing gear
manipulations. The appearance of significant changes in
the prevalence of neoplasia on the Romanian Black Sea
coast can be considered an indicator of chronic stress
(anthropogenic impact), rather than acute
(environmental impact), and we suggest that the species
could be used as a biological indicator of changes that
may occur in the habitat in which it lives. In terms of
future research directions, a combined analysis of the
population structure, morphology and diseases
determined in S. maeoticus populations, with a
structural analysis of the habitat and bacteria
contamination degree would be useful; this monitoring
should be carried out regularly, to reveal changes in
the Black Sea ecosystem, and to propose possible
recommendations and protective measures.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gomez:2023:EBC,
author = "Silvia G{\'o}mez and Gabriele Lara and Carlos Felipe
Hurtado and Ren{\'e} Espinoza Alvarado and Jayro
Guti{\'e}rrez and Jos{\'e} Carlos Huechucoy and
Guillermo Valenzuela-Olea and Alice Turner",
title = "Evaluating the Bioremediation Capacity of the
Polychaete \bioname{Perinereis gualpensis} ({Jeldes},
1963) for \geoname{Atlantic} Salmon Aquaculture
Sludge",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "417",
day = "15",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080417",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/417",
abstract = "The potential of polychaetes for the bioremediation of
aquaculture sludge gained more attention in recent
years. These organisms can reduce organic matter and
nutrients contained in the sludge of several
aquaculture species, improving the sustainability of
these activities. The aim of this study was to evaluate
the removal performance of Perinereis gualpensis
(Jeldes, 1963) being fed with aquaculture sludge
produced by a recirculating system cultivating Atlantic
salmon. The experiment involved adding different
amounts of sludge (10\% and 20\% with respect to total
substrate) at a density of 300 organisms m {$-$2}
during 30 days. A treatment without sludge served as a
control, using natural substrate. The highest removal
rate of total organic matter (TOM) (23.95 {\pm} 13.19 g
m {$-$2} day {$-$1}) was achieved by P. gualpensis with
20\% sludge addition, a reduction of about 36\%
compared to the total amount added at the beginning of
the trials. The organisms fed with aquaculture sludge
presented higher nitrogen (8--9\%) and carbon
(40--43\%) contents, with a maximum organic carbon
assimilation of 32\% in relation to the total content
in the sludge. The high survival (88--95\%) and
positive growth rates (0.28\% day {$-$1}) achieved by
P. gualpensis indicated that this species can be
sustained with salmon sludge as the only source of
food. These results indicate that P. gualpensis is a
promising candidate for removing nutrients from salmon
effluents. Moreover, the protein contents achieved by
the organisms (52--58\%) meet the dietary protein
requirements of several aquaculture species. Further
research is needed to determine the maximum
bioremediation capacity of this species and to evaluate
the lipid content and fatty acid profiles of P.
gualpensis to determine its potential application in
aquaculture feed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wikumpriya:2023:MCG,
author = "Gunasekara Chathura Wikumpriya and Madhuranga
Walawedurage Srinith Prabhatha and Jiye Lee and
Chan-Hee Kim",
title = "Molecular Cloning and Gene Expression of Type {I}
Suppressors of Cytokine Signaling 6 and 7 (\gene{SOCS6}
and \gene{SOCS7}) in Whiteleg Shrimp
(\bioname{Litopenaeus vannamei})",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "416",
day = "14",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080416",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/416",
abstract = "Suppressor of cytokine signaling (SOCS) genes are
essential negative regulators that modulate cytokine
signaling and play key roles in numerous biological
processes, including immune responses. In this study,
we cloned the complementary DNA (cDNA) sequences of two
SOCS genes, designated as Lv SOCS6 and Lv SOCS7, from
the whiteleg shrimp, Litopenaeus vannamei. Lv SOCS6
encoded a polypeptide of 463 amino acids (aas),
spanning 1392 base pairs (bps), while Lv SOCS7 encoded
a significantly larger polypeptide of 955 aas,
encompassing 2868 bps. Both Lv SOCS proteins exhibited
conserved domains associated with SOCS, including a
centralized Src homology 2 (SH2) domain and a
C-terminal SOCS box. Phylogenetic analysis revealed
that the deduced aa sequences of Lv SOCS6 and Lv SOCS7
clustered within the invertebrate type I SOCS family,
indicating their evolutionary relatedness. Tissue
distribution analysis demonstrated ubiquitous
expression of both Lv SOCS genes across all examined
tissues, with Lv SOCS6 showing heightened expression in
the gills and Lv SOCS7 in the gills and stomach.
Notably, mRNA expression patterns of Lv SOCS genes
following LPS and poly (I:C) stimulations exhibited
significant upregulations, while PGN stimulation
yielded incongruous results across the examined
tissues. Interestingly, concurrent with the diminished
expression of Lv SOCS6 and Lv SOCS7, there was
significant elevation in mRNA expression levels of Lv
STAT, a vital component of the Janus kinase
(JAK)/signal transducer and activator of transcription
(STAT) pathway. These findings shed light on the
potential involvement of the newly discovered type I
SOCS genes in regulating the JAK/STAT pathways and
playing pivotal roles in orchestrating the innate
immune responses in L. vannamei defense mechanisms.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kim:2023:DMP,
author = "Eun-Mi Kim and Mi-Nan Lee and Chun-Mae Dong and
Jae-Koo Noh and Eun-Soo Noh and Woo-Jin Kim and Bo-Hye
Nam and Young-Ok Kim",
title = "Development of a Multiplex Polymerase Chain Reaction
Method for Rapid and Accurate Identification of
\bioname{Girella punctata} and \bioname{G. leonina}
({Teleostei: Girellidae})",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "415",
day = "12",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080415",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/415",
abstract = "Girella punctata and Girella leonina are economically
important species found in the East Sea; along the
southern coast of Korea; south of Hokkaido, Japan;
around Taiwan; and in the East China sea. In Korea,
these two species hold high value, particularly on Jeju
Island. These species have similar appearances, and it
is challenging to distinguish them, particularly during
the seed period. We detected genetic differences in the
mtDNA (COI gene) of G. punctata and G. leonina, which
are morphologically indistinguishable, and developed
species-specific genetic markers for their
identification. In total, 16 and 4 haplotypes of the
COI genes were obtained from G. punctata (n = 164) and
G. leonina (n = 36), respectively. The haplotype
diversity (Hd) and nucleotide diversity (Pi, \%) of the
COI were 0.359 and 0.054 for G. punctata and 0.560 and
0.078 for G. leonina, respectively. We designed a
Girella species common primer (control) and
species-specific primer sets (experimental) for the two
species. Amplicon sizes of 991, 579, and 391 bp were
obtained for common primers of the two Girella species
G. punctata and G. leonina. To confirm multiple targets
in a single reaction, multiplex PCR conditions were
optimized to adjust its resolution and accuracy. The
detection levels of the multiplex PCR were confirmed to
be 0.01 ng/{\SGMLmicro}L for the two Girella species.
The multiplex PCR was not associated with
cross-reactivity between G. punctata and G. leonina.
This multiplex species-specific PCR method provides a
simple and rapid technique for the identification of
two Girella species, thus increasing the efficiency and
quality of Girella species stock management and
forensic identification to prevent species
misidentification.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dara:2023:FWA,
author = "Mariano Dara and Pierluigi Carbonara and Claudia {La
Corte} and Daniela Parrinello and Matteo Cammarata and
Maria Giovanna Parisi",
title = "Fish Welfare in Aquaculture: Physiological and
Immunological Activities for Diets, Social and Spatial
Stress on \geoname{Mediterranean} Aqua Cultured
Species",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "414",
day = "12",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080414",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/414",
abstract = "Welfare assessment currently is less
well-characterized for aquatic animals and the
classical methodologies used for terrestrial animals
are not adequate to improve our knowledge about fish
well-being. Among different approaches, the status of
organism responses can be carried out using different
physiological and biochemical tools. Here, we present
the state of the art regarding fish welfare,
methodologies, and experimental results with a
particular focus on two important Mediterranean
aquaculture species, Sparus aurata and Dicentrarchus
labrax. We introduce an approach using physiological
stress-indicators, growth performance and swimming
activity to investigate the effects of the implantation
of electronic tags to facilitate the application of
telemetry for aquaculture purposes. The application of
telemetry to research on aquatic organisms has expanded
recently, and its utilization needs to be better
understood. The mentioned approaches have been
discussed for application in different aquaculture
methodologies. Moreover, social stress and
territoriality are relevant factors in the evaluation
of gregarious species that may have consequences on the
conditions of animals farmed in captivity. These
aspects, that may impair the ability of fish to respond
to various stimuli or negatively influence the flesh
quality, here are analysed through behavioural
observation, flanked by the physiological and
immunological approach.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:OPA,
author = "Shuo Wang and Yingxia He and Feng Xi and Ying Liang
and Shaowei Zhai",
title = "Oligomeric Proanthocyanidins Alleviate the Detrimental
Effects of Dietary Histamine on Intestinal Health of
Juvenile \geoname{American} Eels (\bioname{Anguilla
rostrata})",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "413",
day = "12",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080413",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/413",
abstract = "This study was conducted to evaluate the oligomeric
proanthocyanidins (OPC) in alleviating the detrimental
effects of intestinal health caused by dietary
histamine in juvenile American eels (Anguilla
rostrata). A total of 480 fish with a similar body
weight of 10.84 {\pm} 0.16 g were randomly divided into
four groups, and there were the control group fed a
basal diet, the HIS group fed a diet with a high level
of histamine (534 mg/kg), the H + OPC I group fed the
high histamine diet with 300 mg/kg OPC, and the H + OPC
II group fed the high histamine diet with 600 mg/kg
OPC, respectiviely. After the fish were fed the trial
diets for 77 days, the intestinal samples were taken,
and the related parameters of intestinal health were
analyzed. Dietary 300 mg/kg or 600 mg/kg OPC could
reverse the decreased activities of lipase, protease,
and glutathione peroxidase and the level of total
antioxidant capacity, the increased intestinal
malondialdehyde and D-lactate acid levels and the
activity of diamine oxidase in serum, and the decreased
villus height caused by a high level of dietary
histamine. There were no significant differences above
all the indices between the H + OPC I group and the H +
OPC II group. The higher relative abundances of
potentially pathogenic bacteria were induced by the
high level of dietary histamine. Dietary 300 mg/kg OPC
might increase the relative abundance of the potential
probiotics and inhibit the colonization of intestinal
pathogenic bacteria of juvenile American eels exposed
to the stress of high dietary hisatamine. The
intestinal health status of the H + OPC groups was
similar to that of the control group. These results
suggested that dietary 300 mg/kg OPC might alleviate
the detrimental effects of dietary 534 mg/kg histamine
on the intestine health of juvenile American eels by
increasing the activity of digestive enzymes, improving
the antioxidative potential and barrier function, and
beneficially modulating the intestinal microbiota.
Dietary 600 mg/kg OPC could not exert further
improvement in growth performance and the intestinal
health of juvenile American eels.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Murie:2023:AGF,
author = "Debra J. Murie and Daryl C. Parkyn and Christopher C.
Koenig and Felicia C. Coleman and Christopher R.
Malinowski and Jessica A. Cusick and Robert D. Ellis",
title = "Age, Growth, and Functional Gonochorism with a Twist
of Diandric Protogyny in Goliath Grouper from the
\geoname{Atlantic Coast of Florida}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "412",
day = "11",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080412",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/412",
abstract = "The lack of data on the age, growth, and reproduction
of the Atlantic Goliath Grouper Epinephelus itajara off
the coasts of Florida (USA) makes it difficult to
estimate the extent of their population recovery
following the 1990 fishery closure, as well as the
potential effects of the recent (2023) opening of a
fishery for juveniles. Goliath Grouper from the
Atlantic coast of Florida were non-lethally sampled for
size (total length, TL), age (via fin rays), and sex
and reproductive stage (via gonad biopsies, milt/egg
expression, gonopores) from May to October 2010--2016.
Of 653 unique fish captured (not including 118
recaptures), 257 (39.4\%) were females (122--228 cm TL;
5--20 years old), 264 (40.0\%) were males (104--225 cm
TL; 4--22 years old), 100 (15.3\%) were unsexed
(82--211 cm TL; 3--15 years old), and 32 (4.9\%) were
protogynous hermaphrodites (108--209 cm TL; 5--20 years
old). Protogyny was conclusively determined in two fish
recaptured and re-biopsied that had transitioned from
female to male. However, an overlap in the age and size
of males and females, a 1:1 sex ratio, and the presence
of relatively small, young, mature males, in
combination with an apparently low functional sex
change rate, all indicated that the sexual pattern of
Goliath Grouper was functionally gonochoristic with the
potential for diandric protogyny. Females {$>$} 10
years old were larger-at-age than males, and Goliath
Grouper from the Atlantic coast were larger-at-age than
fish from the Gulf of Mexico. These differences in age,
growth, and reproductive strategy --- as well as the
nascent fishery for juveniles --- need to be monitored
closely so that the current and future reproductive
capacity of the population continues to ensure growth
and sustainability.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Antognazza:2023:CHG,
author = "Caterina M. Antognazza and Anja Palanda{\'c}i{\'c} and
Giovanni B. Delmastro and Giuseppe Crosa and Serena
Zaccara",
title = "Current and Historical Genetic Variability of Native
Brown Trout Populations in a Southern Alpine Ecosystem:
Implications for Future Management",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "411",
day = "10",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080411",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Antognazza:2023:CAA}.",
URL = "https://www.mdpi.com/2410-3888/8/8/411",
abstract = "The highly polymorphic taxon European brown trout
(genus Salmo) has high phenotypic plasticity,
displaying a complex pattern of morphological and
life-history variation, contributing to taxonomic
confusion. Three main mitochondrial lineages (Adriatic,
Mediterranean, and marmoratus) developed during the
Pleistocene climatic events in the southern Alpine
ecosystem. Here, the natural distribution of native
brown trout S. trutta is controversial, complicated by
introductions of the Atlantic strain. By investigating
museum vouchers, this study aimed to retrace the
historical presence of brown trout in the southern
Alpine ecosystem before the beginning of mass
introductions, which occurred since the middle of the
19th century. By examining the combination of
historical and current genetic variability, this study
aims to depict the actual impact of introductions of
the introduced strain, increasing knowledge and
informing conservation strategies and future management
plans. The molecular approaches selected were: (i)
sequencing of the mitochondrial control region and (ii)
genotyping of the nuclear gene LDH-C1 *. Vouchers dated
the presence of the native Adriatic strain since 1821,
while current genetic variability showed the widespread
signature of introgression, a consequence of several
decades of introductions. Focused plans to preserve
local lineages are urgently needed, including
short-term solution to avoid complete pauperization of
this ecosystem.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:DAE,
author = "Yongtao Li and Zhaolong Cheng and Tao Zuo and
Mingxiang Niu and Ruisheng Chen and Jun Wang",
title = "Distribution and Abundance of the \geoname{East Asian}
Finless Porpoise in the Coastal Waters of
\geoname{Shandong Peninsula, Yellow Sea, China}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "410",
day = "10",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080410",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/410",
abstract = "The baseline data pertaining to the population of the
East Asian finless porpoise (Neophocaena
asiaeorientalis sunameri) in Chinese waters are
significantly deficient. Boat-based visual line
transect surveys were conducted from 2018 to 2020 to
evaluate the distribution and abundance of the East
Asian finless porpoise in the coastal waters (depth
{$<$} 30 m) of the Yellow Sea, with a specific focus on
the Shandong Peninsula. A total of 50 transects
covering 2705 km were conducted, encompassing an area
of 23,604 km 2 between {119$^\circ $}30{\prime}
E--{123$^\circ $}15{\prime} E and {35$^\circ
$}25{\prime} N--{38$^\circ $} N. Throughout the
surveys, a total of 117 East Asian finless porpoise
groups were detected, with over 90\% of these groups
occurring within 15 km of the coast or adjacent
islands. The estimated abundance of the East Asian
finless porpoise in the study area was determined to be
3978 individuals (CV = 22.39\%, 95\% CI = 2561--6177),
reflecting a low density of 0.169 individuals/km 2 (CV
= 22.39\%, 95\% CI = 0.109--0.262) within the coastal
waters of the Yellow Sea in the Shandong Peninsula. The
findings highlight the need for conservation measures
to address the threats faced by this species, including
bycatch, habitat degradation, and pollution. The
establishment of marine protected areas and the
implementation of bycatch mitigation measures are
crucial for the long-term survival of the East Asian
finless porpoise population in the study area. By
providing valuable data on the distribution and
abundance of the East Asian finless porpoise, this
study contributes to our understanding of the
population dynamics and conservation status of this
threatened species in the Yellow Sea of the Shandong
Peninsula.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garcia-Marquez:2023:PEM,
author = "Jorge Garc{\'\i}a-M{\'a}rquez and Marta
Dom{\'\i}nguez-Maqueda and Miguel Torres and Isabel M.
Cerezo and Eva Ramos and Francisco Javier Alarc{\'o}n
and Juan Miguel Mancera and Juan Antonio Martos-Sitcha
and Miguel {\'A}ngel Mori{\~n}igo and Mar{\'\i}a Carmen
Balebona",
title = "Potential Effects of Microalgae-Supplemented Diets on
the Growth, Blood Parameters, and the Activity of the
Intestinal Microbiota in \bioname{Sparus aurata} and
\bioname{Mugil cephalus}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "409",
day = "09",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080409",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/409",
abstract = "This work aimed to assess the suitability of a
microalgal blend as a dietary ingredient for feeding
juveniles of marine carnivorous and herbivorous
teleost, as is the case of Sparus aurata and Mugil
cephalus, respectively, and to isolate microorganisms
from different media and characterize them on the base
of their enzymatic activities and their antagonism
against important fish pathogens. Thirty juveniles of
each species (70 {\pm} 3.2 g S. aurata mean weight and
47 {\pm} 2.8 g M. cephalus mean weight) were
distributed in four tanks (15 individuals each)
corresponding to four independent dietary treatments
(control and microalgae diets designed for each
species). Fish were fed their corresponding diets ad
libitum for 108 days. At the end of the trial, fish
were weighed, and plasma, liver, perivisceral fat, and
the entire intestines were obtained for the evaluation
of growth performance and metabolic assessment.
Furthermore, 117 bacterial strains were isolated in
different culture media from the gastrointestinal tract
of S. aurata fed the microalgae blend and further
characterized for their potential use as probiotics in
aquaculture. S. aurata fed the microalgae-supplemented
diet (25\% dietary inclusion) showed a significant
increase in weight gain, specific growth rate, feed
efficiency, hepatosomatic, and intestine length
indices. However, growth performance and somatic
indices in M. cephalus were not affected by the
experimental diets. Plasma samples from S. aurata fed
the microalgal diet revealed higher levels of glucose
and triglycerides and a decrease in cortisol levels. No
significant differences were found in any biochemical
parameters among the experimental diets in M. cephalus.
In conclusion, both species demonstrated a favorable
adaptation to the nutritional formulation employed in
this study, and bacterial strains UMA-169 and UMA-216
(both identified as Bacillus pumilus) could be
considered for use in aquaculture as they might benefit
host health by improving digestion and absorption of
different energy sources and by minimizing the
colonization of pathogenic species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lattos:2023:ADM,
author = "Athanasios Lattos and Dimitrios K. Papadopoulos and
Konstantinos Feidantsis and Ioannis A. Giantsis and
Ioannis Georgoulis and Dimitrios Karagiannis and Basile
Michaelidis",
title = "Antioxidant Defense of \bioname{Mytilus
galloprovincialis} Mussels Induced by Marine Heatwaves
in Correlation with \bioname{Marteilia} Pathogen
Presence",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "408",
day = "08",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080408",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/408",
abstract = "Background: The effects of climate change negatively
affect marine bivalves' health. Lately, the intensity
of marine heatwaves poses serious threats to the
aquatic environment setting of high-risk bivalve
farming. Since temperature increases can jeopardize
bivalves' immunity response, pathogen infection becomes
more evident. Reactive oxygen species (ROS) production,
increased during the process of phagocytosis, is
confronted by the animals' antioxidant defense system.
However, apart from pathogenic infections, antioxidant
defense responses are also induced by seawater
temperature increases; Methods: To enlighten the
antioxidant status of Mytilus galloprovincialis
originating from mortality events enhanced by intense
heatwaves in Thermaikos Gulf, northern Greece, along
with Marteilia refringens infection, we examined the
expression of genes related to antioxidant defense
(catalase, CuSOD and mt10) along with the lipid
peroxidation levels and activity levels of antioxidant
enzymes (catalase, SOD and glutathione reductase);
Results: Our results exhibited increased levels of all
these biomarkers. This increase was intensified in the
Marteilia infected individuals; Conclusions: Our
findings shed light on the oxidative and antioxidant
status of farmed mussels led to mortality in the
context of Marteilia infection. The latter is augmented
by the synergistic effect of heatwaves causing a
significant increase in oxidative damage and subsequent
antioxidant defense.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fedder:2023:SDM,
author = "Jens Fedder",
title = "Sex Determination and Male Differentiation in Southern
Swordtail Fishes: Evaluation from an Evolutionary
Perspective",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "407",
day = "06",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080407",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/407",
abstract = "Southern swordtail fishes, which belong to the
viviparous teleosts called Xiphophorus, are unique
models for studies of evolution of sex chromosomes.
Monofactorial sex-determining systems, with either the
male or the female being the heterogametic sex, as well
as sex determination involving more than two sex
chromosomes, are found in swordtails and related
species. Some swordtail species seem to have originated
by crossing between two closely related species.
Although the sword has disappeared in many Xiphophorus
species during evolution, females of non-sworded
species still prefer sworded males, demonstrating a
discrepancy between natural and sexual selection.
Natural sex change has not been documented sufficiently
convincingly in swordtails, but, at least in some
subspecies, two or more male phenotypes exist. In a
laboratory experiment performed for over 30 years, it
has been observed that sex-determining genes may be
translocated from one chromosome to another in hybrids
of these evolutionary young species. While the factors
suggested to play central roles in sex determination
and differentiation, e.g., Dmrt1 and AMH, are highly
conserved during evolution, several master determining
factors have been detected in teleosts.
Endocrine-induced sex reversal has been demonstrated in
the guppy Poecilia reticulata, another viviparous fish.
In swordtails (X. helleri), endocrine disruptors such
as nonylphenol and bisphenol A may cause testis cell
degeneration and the inhibition of spermatogenesis.
Furthermore, swordtails are very easy to breed in
freshwater aquaria and, therefore, may be good models
for studying the factors influencing sex determination
and male differentiation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Schemmel:2023:HEH,
author = "Eva Schemmel and Nancy J. Brown-Peterson",
title = "Handling Effects on Histological Identification of
Female Reproductive Status: Examples from Tropical
Deepwater Snappers",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "406",
day = "04",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080406",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/406",
abstract = "Post-mortem ovarian tissue degradation from handling
effects, such as delays in preservation, can lead to
inaccurate reproductive assessments. Therefore, it is
important to understand the differences between
handling effects and natural ovarian atresia. Seven
treatments of various holding temperatures and times to
preservation were applied to whole fish and extracted
ovaries of three tropical deepwater snapper species.
Ovarian post-mortem degradation was observed at 12 h at
ambient temperature and after 48 h iced or refrigerated
for both whole fish and extracted ovaries; however,
degradation did not adversely affect correct
reproductive phase classification in fishes stored in
ice from 12 to 48 h post-capture. A clear histological
indicator of post-mortem degradation was the breakdown
of ooplasm organelles at the oocyte periphery observed
in vitellogenic and cortical alveolar oocytes. However,
the chorion typically remains intact during post-mortem
degradation, in contrast to natural atresia. Effects on
post-ovulatory follicles were most discernible after 96
h on ice or refrigeration. Freezing gonadal tissue
resulted in drastic changes to oocyte shape and
morphology, making reproductive phase classification
challenging. We advise that (1) freezing ovaries should
be avoided where possible, and (2) gonad tissue should
ideally be preserved immediately but no later than 48 h
post-capture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wan:2023:ELP,
author = "Shuai Wan and Zhaosheng Sun and Chang Zhang and
Tingshuang Pan and Shuya Yuan and Yuxi Chen and Jun Zou
and Qian Gao",
title = "Effects of {LPS}, Poly ({I:C}) and
\bioname{Edwardsiella tarda} on the Expression Patterns
of {IL-17} Family Members and Their Receptors in
Spotted Sea Bass (\bioname{Lateolabrax maculatus})",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "405",
day = "04",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080405",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/405",
abstract = "In fish, the immune system plays a crucial role in
defending against pathogen-induced infections. The
interleukin 17 (IL-17) family, which is a well-studied
class of cytokines, serves as a key component of the
immune response against external pathogens. In this
research, four IL-17 ligands (IL-17A/F1, IL-17B, IL-17C
and IL-17D) and one receptor (IL-17RB) genes were
identified from spotted sea bass (Lateolabrax
maculatus). Alignment analysis showed that the
C-terminal region of IL-17 ligands in spotted sea bass
was highly conserved. The expression of the IL-17
ligand and receptor genes differed in unstimulated
tissues. To investigate the impact of various factors
on the immune response of spotted sea bass, we assessed
the effect of lipopolysaccharide (LPS),
polyinosinic--polycytidylic acid [poly (I:C)] and
Edwardsiella tarda treatment on the IL-17 ligands and
receptor responses. Our results reveal that the
expression of the IL-17 ligand and receptor transcripts
is modulated by LPS, poly (I:C) and E. tarda,
indicating their significant role in the immune
system.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Batista:2023:SLS,
author = "Rosana Oliveira Batista and Bianca Leticia Richter and
Jorge Filipe Banze and Delano Dias Schleder and Maria
Salhi and Renata Oselame Nobrega and Maria Fernanda
Oliveira da Silva and Bruna Mattioni and James Eugene
Pettigrew and D{\'e}bora Machado Fracalossi",
title = "Soy Lecithin Supplementation Promotes Growth and
Increases Lipid Digestibility in {GIFT} \geoname{Nile}
Tilapia Raised at Suboptimal Temperature",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "404",
day = "03",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080404",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/404",
abstract = "Soy lecithin (SL) is a source of phospholipids, which
play a crucial role in determining cell membrane
structure, fluidity, and functionality. This study
investigated the effects of dietary SL on the
performance, nutrient digestibility, and body
composition of Nile tilapia juveniles (average initial
weight 12.2 g) raised at 22 {$^\circ $}C. The
experimental diets contained increasing levels of SL
(0.0, 21.0, 43.0, and 64.0 g kg {$-$1}). The best
weight gain and feed efficiency occurred with 42.2 and
49.8 g kg {$-$1} of SL inclusion, respectively,
estimated through quadratic regression after 90 days of
feeding. The body composition of the fish was also
affected by feeding with SL, with a decrease in total
body lipids and viscerosomatic and hepatosomatic
indices but an increase in polyunsaturated fatty acids.
A digestibility trial using only two diets (0SLD and
43SLD) revealed increased lipid and fatty acids
digestibility in fish fed with 43SLD. In addition, the
consumption of digestible nutrients was the highest in
fish fed the diet 43SL. Therefore, SL supplementation
is beneficial in fall/spring diets for Nile tilapia
juveniles as it can improve growth, lipid
digestibility, and body composition when supplemented
within the range of 42 to 50 g kg {$-$1} diet.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fitzpatrick:2023:EPF,
author = "Ryan M. Fitzpatrick and David W. Longrie and Ryan J.
Friebertshauser and H. Paul Foutz",
title = "Evaluation of a Prefabricated Fish Passage Design for
\geoname{Great Plains} Fishes",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "403",
day = "03",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080403",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/403",
abstract = "Connectivity is critical for stream fish persistence,
and fish passage structures are a useful conservation
tool to reconnect fragmented systems. The design of
fish passage structures is a tradeoff between the area
available for construction, slope, and costs associated
with the structure. The Longrie--Fecteau fish passage
structure was designed to be modular, adjustable to
barrier-specific needs, and to have a low slope (2\%)
to pass small-bodied fishes. We evaluated fish passage
through this structure in Fountain Creek, Colorado,
USA, via a PIT tag mark--recapture study. We documented
four native Great Plains fish species successfully
ascending the passage structure, with most passage
occurring at night. We estimated a 3\% probability of a
released fish entering the structure, then 89\% and
99\% passage to the midpoint and exit of the 123 m
structure, respectively. Low entrance efficiency was
due to low recapture probability of small-bodied study
organisms in a relatively large system, and the low
percentage of space of the entryway on this barrier
({$<$3}\% of the length of the barrier). Fish that
entered the structure ascended quickly, with median
time for successful ascent of 19 min, and minimum time
of 6 min. The Longrie--Fecteau fish passage structure
is a conservation tool that may broaden the adoption of
fish passage structures for small-bodied fishes due to
its modularity and low slope.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Akgun:2023:RTB,
author = "Yagmur Akgun and Ekin Akoglu",
title = "{Randall}'s Threadfin Bream (\bioname{Nemipterus
randalli}, {Russell} 1986) Poses a Potential Threat to
the \geoname{Northeastern Mediterranean Sea} Food Web",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "402",
day = "03",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080402",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/402",
abstract = "The eastern Mediterranean Sea is one of the most
invaded marine ecosystems due to the introduction of
Lessepsian species, which migrated from the Red Sea to
the Mediterranean Sea following the construction of the
Suez Canal. Some of these species may initially appear
to be beneficial for fisheries by providing additional
income sources for fishers; however, this usually
occurs at the expense of negatively impacted native
species and, thus, the ecosystem, which leads to
greater economic losses for the fisheries in the long
term. Therefore, this study aims to quantify the impact
of N. randalli, which is one of the Lessepsian species
with increasing commercial importance for the
fisheries, on the food web dynamics in a coastal
ecosystem in the northeastern Mediterranean Sea using a
mass-balance food web modelling approach by
capitalising on field data obtained from trawl
samplings conducted within the scope of the study.
Results showed that the ecosystem was in a
developmental stage and experienced an autotrophic
succession. The keystone fish group with a structuring
role in the food web was sea breams and porgies.
Although N. randalli had positive impacts on certain
commercially exploited indigenous demersal fish species
by mitigating the negative impact of another Lessepsian
species, i.e., Saurida undosquamis (Richardson, 1848),
in the food web, it had a negative impact on the
keystone group of the food web, i.e., sea breams and
porgies. Therefore, N. randalli poses a potential
threat to the ecosystem's structure, and the
interactions of N. randalli with other species in the
food web may instigate an ecosystem reorganisation in
the future. We suggest targeted fisheries exploitation
and incentives for the fishery of N. randalli as
management strategies to mitigate its negative impacts.
However, the mitigating role of N. randalli in
regulating the negative impacts of S. undosquamis could
be adversely affected by its increasing exploitation;
therefore, future modelling studies should consider
scenario simulations to test such effects.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mao:2023:FNP,
author = "Xiao-jiang Mao and Kang-le Lu",
title = "Fish Nutrition and Physiology",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "401",
day = "02",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080401",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/401",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tserkova:2023:BPB,
author = "Feriha M. Tserkova and Vesselina V. Mihneva and Elitsa
P. Petrova-Pavlova",
title = "Biological Parameters and Biomass and Abundance
Indices of Two Demersal Species, Turbot
(\bioname{Scophthalmus maximus}) and Thornback Ray
(\bioname{Raja clavata}), Estimated by a Trawl Survey
in \geoname{Western Black Sea}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "400",
day = "02",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080400",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/400",
abstract = "In this study, we determined the biological
characteristics and indices of abundance and biomass of
two demersal species, turbot and thornback ray, through
a scientific trawl survey of Bulgarian Black Sea waters
in the autumn of 2020. Turbot is among the most
valuable fish species in the Black Sea, and thornback
ray is a sensitive cartilaginous species with a
significantly declining abundance throughout the
Mediterranean region. The stock biomass of turbot was
estimated at 1467.6 tons, with a relative abundance of
896,922 individuals, and those of the thornback ray
were 1187.9 tons and 519,606 individuals, respectively.
Mean turbot abundance, interpolated into {0.5$^\circ $}
latitude/longitude grid cells, ranged between 52 and
120 ind{\cdot}km {$-$2}, with a mean biomass of
78.26--238.31 kg{\cdot}km {$-$2}, and for the thornback
ray, these indices were within the limits of 0--107
ind{\cdot}km {$-$2} and 0--219 kg{\cdot}km {$-$2}. The
distribution of the different length classes of the two
fish species by depth layer was analyzed.
Length--weight relationships were estimated based on
combined samples of both sexes and separately for
female and male individuals, allowing a better
understanding of growth patterns.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Samy-Kamal:2023:MET,
author = "Mohamed Samy-Kamal and Tatiana Shulezhko and Natalia
Lisitcyna",
title = "Marine Endangered and Threatened Species in
\geoname{Russia}: a Review of Current Conservation
Strategies and Management Legislative Tools",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "399",
day = "02",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080399",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/399",
abstract = "Despite the global decline in marine species
biodiversity, relatively few countries have enacted
national endangered and threatened species legislation.
Tailoring an adequate legislative framework with clear
objectives and regulations consistent with the
available scientific evidence is fundamental for the
effective conservation of marine endangered and
threatened species. This paper analyzes the legal
framework and current institutional tools for the
conservation of marine endangered and threatened
species in the Russian Federation. In this regard,
important legislative tools include federal laws, as
well as internationally binding signed agreements,
among others, such as the Convention on International
Trade in Endangered Species of Wild Fauna and Flora
(CITES) and the Convention on Wetlands of International
Importance Especially as Waterfowl Habitat (Ramsar). A
strategy and an action plan for the conservation of
biological diversity were also developed. Besides, the
most important tool for the conservation and protection
of marine endangered and threatened species is the Red
Book of the Russian Federation (RBRF) and other
regional Red Books. Responsibility for causing harm to
the species listed in the RBRF and their habitat is
specified in the code of administrative offenses and
the criminal code of the Russian Federation. Finally,
conclusions and identified gaps were highlighted in the
last section, including, among other things, that
legislation is still limited in how it takes the
impacts of climate change into account. Such type of
study is highly recommended, considering the relatively
few number of papers dedicated to the study of the
impact and/or implications of the conservation tools
and strategies mentioned in this paper on the status of
the marine endangered and threatened species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ellis:2023:SMA,
author = "Robert D. Ellis and Christopher C. Koenig and James V.
Locascio and Christopher R. Malinowski and Felicia C.
Coleman",
title = "Spawning Migrations of the \geoname{Atlantic} Goliath
Grouper along the \geoname{Florida Atlantic Coast}",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "398",
day = "01",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080398",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/398",
abstract = "Atlantic goliath grouper (Epinephelus itajara), the
largest reef fish in the Western Atlantic, exhibit high
site fidelity to home reefs but also undertake annual
migrations to distant spawning sites. Once relatively
common throughout Florida and the Caribbean, the
species; is now considered vulnerable (i.e., threatened
with extinction) due to overfishing and loss of
juvenile mangrove habitat. Goliath grouper in the
southeastern US form annual spawning aggregations on
high-relief reefs located offshore of both the Gulf and
Atlantic coasts of Florida, US. To determine spawning
site fidelity and describe migration patterns to
aggregations, we implanted 50 adult goliath grouper
with acoustic transmitter tags from 2010 to 2013. Fish
were tagged at known spawning sites off the Florida
Atlantic coast and tracked as they moved through the
FACT Network array of acoustic receivers. From 2010 to
2020, we collected $ \approx $7 million detections from
tagged goliath grouper at 153 sites along the
southeastern US Atlantic coast. Results of this
long-term tracking indicate that adult goliath grouper
are relatively sedentary during non-spawning months
(Nov to June) but move significantly more prior to,
during, and immediately after spawning (July to Oct).
Inter-annual spawning site fidelity was high: between
80--93\% of tagged fish returned to the same spawning
sites each year. Arrival timing at spawning sites
coincided with the August new moon, with males arriving
earlier than females. Some individuals migrated
distances greater than 400-km per year, with observed
migration rates of up to 44-km per day prior to
spawning. Long-term tagging data are critical for
understanding movement patterns and developing
management strategies for this species of special
conservation concern.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shi:2023:EFG,
author = "Lian Shi and Junjie Zhang and Lingmin Zhao and Qi Li
and Lixing Huang and Yingxue Qin and Qingpi Yan",
title = "Effect of the Flagellar Gene \gene{fliL} on the
Virulence of \bioname{Pseudomonas plecoglossicida} to
Hybrid Grouper (\bioname{Epinephelus fuscoguttatus}
[female sign] $ \times $ \bioname{E. lanceolatus} [male
sign])",
journal = j-FISHES,
volume = "8",
number = "8",
pages = "397",
day = "01",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8080397",
ISSN = "2410-3888",
bibdate = "Thu Sep 14 11:07:45 MDT 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/8/397",
abstract = "Pseudomonas plecoglossicida is the pathogen of
visceral white spot disease in marine fish, which
usually occurs at 16--19 {$^\circ $}C and has resulted
in heavy economic losses. Our previous RNA sequencing
revealed that the expression of the fliL gene in P.
plecoglossicida was significantly up-regulated during
infection of the host. In order to study the influence
of the fliL gene on the virulence of P.
plecoglossicida, the fliL gene of the NZBD9 strain was
knocked out by the homologous recombination method, the
fliL gene-deleted strain ({\Delta} fliL strain)
constructed, and complemented the fliL gene to the
{\Delta} fliL strain to obtain the C-{\Delta} fliL
strain. The growth curves of the NZBD9 strain, {\Delta}
fliL strain, and C-{\Delta} fliL strain did not show
significant differences. Compared with the NZBD9
strain, the motility, adhesion, and biofilm formation
ability were tendered in the {\Delta} fliL strain (p
{$<$} 0.05); the complement of the fliL gene enhanced
these abilities to the level of the NZBD9 strain. The
results of artificial infection experiments showed that
the LD 50 of NZBD9 strain, {\Delta} fliL strain, and
C-{\Delta} fliL strain in hybrid grouper (Epinephelus
fuscoguttatus {[female-sign]} $ \times $ E. lanceolatus
{[male-sign]}) were 5.0 $ \times $ 10 3 CFU/fish, 6.3 $
\times $ 10 4 CFU/fish, and 1.3 $ \times $ 10 3
CFU/fish, respectively. RNA sequencing was performed on
wild-type strains and {\Delta} fliL strains. A total of
126 differentially expressed genes (DEGs) were screened
(p {$<$} 0.05), of which 114 were downregulated and 12
were upcontrolled, among which several genes related to
the six-type secretion system and transport activity
were significantly downregulated. The DEGs were aligned
to the GO and KEGG databases and enriched to 44 GO
pathways and 39 KEGG pathways, respectively. The active
pathways of ABC transporters were significantly
enriched in both databases. These results indicate that
the fliL gene is related to the movement, biofilm
formation, and adhesion ability of P. plecoglossicida,
and may reduce virulence by affecting substance
transport and bacterial secretion.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:CDI,
author = "Hongxiang Xu and Jindong Ren and Xiaojun Xu and Bao
Lou and Demin Zhang",
title = "The Composition and Diversity of Intestinal Microbes
at Different Growth Stages of Giant Freshwater Prawns
(\bioname{Macrobrachium rosenbergii})",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "473",
day = "21",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090473",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/473",
abstract = "Intestinal bacterial communities play an important
role in the growth and health of aquatic animal hosts
and have drawn increasing attention. However, the role
of the intestinal microbiota in the growth of
freshwater prawns remains unclear. Here, the intestinal
microbiota of freshwater prawns (Macrobrachium
rosenbergii) at different life stages (one, two, and
three months old) were investigated using 16S rRNA
sequencing. The results showed that community richness
and diversity increased with growth, which might be one
of the reasons that the prawns maintained a fast growth
rate before sexual maturation. Three core phyla were
identified in the one-month-old group, namely,
Firmicutes (79.24\%), Proteobacteria (17.09\%) and
Actinobacteriota (2.01\%). Five core phyla were
identified in the two-month-old group, including
Firmicutes (47.84\%), Proteobacteria (44.22\%),
Actinobacteriota (1.83\%), Acidobacteriota (1.66\%) and
Bacteroidota (1.24\%), and the core phyla in the
three-month-old group were similar, except for
Acidobacterota, which was not identified. A total of 12
core genera were identified in all samples, and
significant differences were observed in the relative
abundance of gut microbiota between the three groups (p
{$<$} 0.05). Exiguobacterium, Lactococcus and
Shewanella were the three most significantly
differentially abundant genera between stages. In
addition, Candidatus Hepatoplasma was detected only in
the two- and three-month-old prawn groups. This study
provides information on the differences in the
intestinal microbiota in different developmental
stages, which contribute to adaptation to salinity in
the early developmental stage and digestive ability to
meet the growth needs of Macrobrachium rosenbergii.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Aviles-Polanco:2023:CES,
author = "Gerza{\'\i}n Avil{\'e}s-Polanco and Marco Antonio
Almendarez-Hern{\'a}ndez and Luis Felipe
Beltr{\'a}n-Morales and Fernando Aranceta-Garza",
title = "Cost Efficiencies of the Shrimp Fishery in
\geoname{Mexico}: a Stochastic Frontier Analysis",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "472",
day = "21",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090472",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/472",
abstract = "Fishing sector fuel subsidies are designed to increase
profitability by reducing costs. However, despite the
number of liters of fuel subsidized in 2018 in Mexico,
there is no information available on the effectiveness
of the subsidies in reducing cost inefficiencies. The
purpose of this study was to estimate the cost
efficiency of shrimp fishing companies in Mexico, as
well as measure the impact of fuel subsidies on the
cost inefficiency of the sector from 2003 to 2018. The
True Fixed Effects model was used to represent a
Cobb--Douglas stochastic production frontier, which
included a shrimp fishing inefficiencies model. The
results indicate that shrimp fishing companies could
reduce their costs by 25\% without reducing their catch
levels. Fishing companies in the Gulf of Mexico were
more efficient than those operating in the Gulf of
California and the South Pacific. Fuel subsidies reduce
cost inefficiencies, with a greater effect when the
subsidy reaches a level of 20\% of the total liters of
subsidized fuel.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Linnansaari:2023:RCW,
author = "Tommi Linnansaari and Ant{\'o}in M. O'Sullivan and
Cindy Breau and Emily M. Corey and Elise N. Collet and
R. Allen Curry and Richard A. Cunjak",
title = "The Role of Cold-Water Thermal Refuges for Stream
Salmonids in a Changing Climate --- Experiences from
\geoname{Atlantic Canada}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "471",
day = "21",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090471",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/471",
abstract = "Thermal refuges are becoming increasingly influential
for dictating the population status and spatial
distribution of cold-water stenotherm salmonids in the
mid- to southern extent of their range. The global
climate is predicted to continue to warm, and
therefore, the overall thermal suitability of
freshwater habitats for stream salmonids is predicted
to decline in concert. However, stream and river
thermal heterogeneity will offer considerable
resiliency for these populations. Thermal refuges are
formed by many physical processes; common natural
refuges include cold tributary plumes, groundwater
springs, alcoves, and hyporheic upwellings. However,
many anthropogenically formed refuges (such as
stratified reservoirs or cold-water tailrace outflows)
also exist in hydropower-regulated rivers. The
significance of these refuges to stream salmonids
depends on their size and temperature differential, but
also other habitat characteristics such as their depth,
flow velocity, Froude number, and many biotic factors
within the refuges. Modern technologies such as
drone-mounted thermal infrared cameras and other remote
sensing techniques allow for the efficient
identification of such refuges, and inexpensive options
include the identification of refuges during ice cover
using orthophotographs. Behavioural thermoregulation,
i.e., salmonids aggregating in cold-water refuges, can
be either facultative or obligate and the timing of
these events is governed by life stage, species, and
population-specific physiologically regulated
cumulative thresholds that are inherently related to
the recent thermal history, or hysteresis, of each
individual. Salmonids appear to have an excellent
spatial cognition for locating and relocating
cold-water refuges, and their spatial distribution is
largely affected by the availability of the cold-water
refuges during the warm-water period in many thermally
stressed rivers. Gregarious behaviour is the norm for
salmonid fishes within the thermal refuges; however,
the size/microhabitat hierarchy appears to dictate the
within-refuge distribution at the micro-scale. There
continues to be a great impetus for protecting---and in
carefully determined cases creating---cold-water
refuges in the future. A thorough understanding of what
a ``goldilocks'' refuge is for various salmonids and
their different life stages will be imperative as
cold-water restoration is gaining popularity. Finally,
disentangling the roles of the climate-induced and
landscape activity-induced warming potential of fluvial
freshwater will be important to ensure continued
environmentally responsible landscape activities in
future waterscapes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huo:2023:SAE,
author = "Yilin Huo and Dazhang Yang and Jing Xie and Zhikang
Yang",
title = "Simulation Analysis and Experimental Verification of
Freezing Time of Tuna under Freezing Conditions",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "470",
day = "21",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090470",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/470",
abstract = "In order to predict the regular temperature change in
tuna during the freezing process for cold chain
transportation, improve the quality of frozen tuna, and
reduce the energy consumption of freezing equipment, a
three-dimensional numerical model for freezing tuna of
different sizes was established. An unsteady numerical
simulation of the air velocity and flow field was
combined with an analysis of the freezing process of
tuna. This paper also studied the effect of air
velocity, temperature, and tuna size on the freezing
process. The numerical results show that there was a
positive correlation between the cold source
environment and the tuna-freezing process. Lower
temperatures and higher air increased the velocity at
which the tuna moved through the maximum ice crystal
formation zone, maintaining a better aquatic product
quality. In some cases, however, the smaller tuna
models achieved a longer freezing time. Due to the
difficulty of obtaining the whole tuna sample, the
temperature curve and freezing rate over time obtained
during the freezing process were tested using a tuna
block of a specific size. The maximum error did not
exceed 6.67\%, verifying the authenticity and
feasibility of the simulation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Binstock:2023:ITF,
author = "Addie L. Binstock and Audrey S. Fox and John A.
Mohan",
title = "Isotopic Turnover and Fractionation of {$ \delta^{15}
$N} and {$ \delta^{13} $C} in Captive
\bioname{Pseudopleuronectes americanus} ({Walbaum})",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "469",
day = "21",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090469",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/469",
abstract = "Stable isotope ratios of nitrogen ({\delta} 15 N) and
carbon ({\delta} 13 C) are ubiquitous ecological
tracers used to elucidate an organism's diet and
habitat. However, the application of stable isotope
ratios to reconstruct a consumer's ecology relies upon
accurate rates for isotopic turnover at both a tissue
and species-specific level. This study estimated
isotope turnover rates and trophic discrimination
factors in four different tissues (liver, digestive
tissue, muscle, and skin) with variable metabolic
activity in winter flounder Pseudopleuronectes
americanus using a controlled diet-switch experiment.
Differences in half-lives were noted among the tissues
and between the experimental diets for both {\delta} 15
N and {\delta} 13 C. The experimental diets of krill
and mysis had variability in nutritional composition,
resulting in similar turnovers in {\delta} 15 N but
slower turnovers in {\delta} 13 C for fish fed krill.
Turnovers in both {\delta} 15 N and {\delta} 13 C were
strongly influenced by metabolism, with the
contribution reaching up to 98\%, as fish exhibited
minimal overall growth. The results of this study
demonstrate the importance of considering differences
in the catabolic activity of tissue maintenance for
fish exhibiting minimal growth, as well as differences
in metabolic assimilation of dietary sources that vary
in their protein and lipid contents.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nguyen:2023:RFH,
author = "Van Bon Nguyen and San-Lang Wang and Thi Hanh Nguyen
and Tu Quy Phan and Thi Huyen Nguyen and Thi Ha Trang
Tran and Manh Dung Doan and Van Anh Ngo and Anh Dzung
Nguyen",
title = "Recycling Fish Heads for the Production of
Prodigiosin, a Novel Fungicide via Experimental and
Molecular Docking Characterization",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "468",
day = "19",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090468",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/468",
abstract = "This report aims to characterize and reuse fish head
powder (FHP) as a potential carbon/nitrogen source for
microbial fermentation to produce prodigiosin (PG), and
to evaluate its novel anti-fungal effect via an
experimental and docking study. FHP has high protein
(30.25\%) and lipid (5.12\%) content and a diversity of
elements, which suggests that FHP is a potential
substrate for microbial fermentation. Serratia
marcescens TNU01 was screened as the most effective
strain to produce PG from FHP. Further experiments
indicated that the combination of FHP and crude chitin
at the ratio of 9/1 and used at 1.5\% as a C/N source
for fermentation significantly enhanced PG production.
Supplementing a minor amount of 0.05\% Ca 3 (PO 4) 2,
0.03\% K 2 SO 4 also positively affects PG yield
production. The fermentation using a bioreactor system
(type of 14 L) achieved the highest PG yield production
(6.4 mg/mL) in a short cultivation time (10 h) in
comparison with the highest PG yield (5.8 mg/mL)
obtained after 36 h of fermentation in a flask. In the
anti-fungal evaluation of various plant pathogen fungal
strains, PG demonstrated efficacy against Fusarium
solani F04. The molecular docking results also showed
that PG effectively interacted with targeting protein
3QPC of F. solani with good binding energy (DS---9.2
kcal/mol) and an acceptable RMSD value (0.94 {\AA}). In
this study, we provide a novel application of FHP for
the eco-friendly production of PG, which can
potentially used for the management of Fusarium
solani.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2023:HDD,
author = "Xuanyi Yang and Xinwei Guo and Xiaohui Dong and Qihui
Yang and Hongyu Liu and Shuang Zhang and Beiping Tan
and Shuyan Chi",
title = "How Do Different Dietary Carbohydrate\slash Lipid
Ratios Influence Intestinal Morphology and Glycolipid
Metabolism Capacity in Hybrid Grouper
(\bioname{Epinephelus fuscoguttatus} [female sign] $
\times $ \bioname{E. lanceolatus} [male sign])",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "467",
day = "19",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090467",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/467",
abstract = "Levels of dietary carbohydrates and lipids have
important effects on the growth, health, and glycolipid
metabolizing capacity of the hybrid grouper
(Epinephelus fuscoguttatus {[female-sign]} $ \times $
E. lanceolatus {[male-sign]}). This experiment
evaluated the effect of carbohydrate to lipid ratios
(CHO:L ratio) on growth, serum biochemical indices,
intestinal morphology and activity of digestive
enzymes, and the ability to metabolize carbohydrates
and lipids in the hybrid grouper. Six iso-nitrogenous
(500 g/kg) and iso-energetic (190 MJ/kg) feeds with
CHO:L ratios of 0.82, 1.03, 1.28, 1.58, 1.94, and 2.27
were formulated. The 720 groupers with an initial body
weight of 7.76 {\pm} 0.08 g were sorted into 24
fiberglass buckets of 30 fish each. Feed was delivered
twice daily at 8:00 and 16:00 to apparent satiety for
56 days. The results showed that the growth performance
of the groupers was not significantly affected by the
diet. Based on the specific growth rate (SGR), the
optimal CHO:L ratio was 1.72 for the groupers by the
second-order polynomial regression model. The
intestinal trypsin and lipase, and the hepatic trypsin
activities of the groupers showed a maximum in the 1.58
group. The intestinal muscle thickness in the 1.94
group and the villus length in the 1.58 group were
significantly greater than in the 0.82, 1.03, 1.28, and
2.27 groups. The glycogen synthase, pyruvate kinase,
and hexokinase activities of the liver were
significantly higher in the 1.58 group than in the 0.82
and 2.27 groups. The hormone-sensitive triglyceride
lipase, adipose triglyceride lipase, acetyl-CoA
carboxylase, and carnitine palmitoyl transferase of the
liver showed significantly higher activities in the
1.58 group than those in the 0.82 and 1.03 groups.
These results showed that based on the SGR of the
groupers, the best CHO:L ratio was 1.72. An appropriate
dietary CHO:L ratio can reduce the lipid deposition of
the fish body, liver, and muscle, as well as improve
the intestinal morphology and activities of liver
carbohydrate and lipid metabolism enzymes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:EFC,
author = "Zhanzhan Wang and Shuling Liao and Jun Wang and Yun
Wang and Zhong Huang and Wei Yu and Xiaolin Huang and
Heizhao Lin and Maoyan Luo and Zhenyan Cheng and
Chuanpeng Zhou",
title = "Effects of Fermented Cottonseed Meal Substitution for
Fish Meal on Intestinal Enzymatic Activity,
Inflammatory and Physical-Barrier-Related Gene
Expression, and Intestinal Microflora of Juvenile
Golden Pompano (\bioname{Trachinotus ovatus})",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "466",
day = "18",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090466",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/466",
abstract = "The present study was conducted to investigate the
effects of dietary fermented cottonseed meal (FCSM)
substitution for fish meal on intestinal enzymatic
activity, inflammatory and physical-barrier-related
gene expression, and intestinal microflora of juvenile
golden pompano. The 375 golden pompanos were divided
into 15 groups of 25 fish each, with three replicates
for each experimental group. The fish were fed five
experimental diets (0 (FM), 12.5\% (CSM12.5), 25\%
(CSM25), 50\% (CSM50), and 100\% (CSM100) substitution
levels) for 8 weeks. The fish were reared and fed the
experimental diets under a natural-day light cycle.
Compared with the control group, the activities of AMY
(amylase) enzymes in the CSM12.5 group and all other
groups were elevated (p {$<$} 0.05). The CSM25 group
exhibited a considerable up-regulation of IL-10
(Interleukin-10) expression relative to the FM group (p
{$<$} 0.05). With an increase in dietary FM
substitution with FCSM from 0 to 25\%, the relative
expressions of NF-{\kappa}B (Nuclear factor kappa-B),
IL-1{\beta} (Interleukin-1 beta), and IL-8
(Interleukin-8) were down-regulated. In this study, the
relative expressions of ZO-1 (zonula occluden-1) and
Occludin were up-regulated, and those of Claudin-3 and
Claudin-15 significantly up-regulated, when the FCSM
substitution ratio was 25\%. The results of
high-throughput sequencing of the intestinal microflora
showed that ACE indices the lowest in the CSM25 group,
which was significantly different from those in the
CSM100 group (p {$<$} 0.05). The CSM50 group had the
highest Shannon and Simpson indices and the highest
community diversity. In addition, replacing a high
percentage of fish meal with FCSM can negatively affect
the intestinal flora of fish. In this study, the 25\%
substitution ratio improved nutrient absorption,
reduced intestinal inflammation, improved intestinal
physical barrier damage, did not affect intestinal
microecology, and had no adverse effects on fish.
However, substitution of a high proportion of FM with
FCSM negatively affects the intestinal microflora and
nutrient absorption capacity of fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:OUO,
author = "Changwei Li and Xiaoyu Zhang and Yu Chen and Shiyu
Zhang and Limin Dai and Wenjing Zhu and Yuan Chen",
title = "Optimized Utilization of Organic Carbon in Aquaculture
Biofloc Systems: a Review",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "465",
day = "16",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090465",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/465",
abstract = "Biofloc technology (BFT) as an efficient aquaculture
alternative is attracting attention for boosting
biosecurity with minimal water exchange and reduced
feed intake. BFT relies on applying organic carbon to
maintain a high C/N ratio above 10, which allows
heterotrophs to assimilate toxic nitrogen. A high
percentage of carbon loss is thus generated from BFT.
For this review, a thorough search of the relevant
literature was conducted to gather valuable information
on the optimization of carbon utilization. The keywords
searched included `BFT', `carbon use efficiency',
`carbon conversion', `carbon retention', `carbon
emission', `carbon loss', and `carbon release'. The
current review discusses the possible effects of
various C/N ratios, carbon types, addition strategies,
and technology integration with respect to the
optimized carbon utilization in BFT. Given the extreme
lack of accessible research, it was concluded that the
carbon utilization in BFT is still in its initial
research stage. Anyhow, this review sheds light on
plausible approaches for boosted carbon utilization by
adopting slow-release carbon, reducing carbon input,
integrating with other technologies, and enhancing
interactions between functional microbes within BFT,
thereby contributing to sustainable aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cheng:2023:CEM,
author = "Zhaohai Cheng and Lei Gao and Lixiong Yu and Xinbin
Duan and Fengyue Zhu and Huiwu Tian and Daqing Chen and
Mingdian Liu",
title = "Catch Efficiency of Multi-Mesh Trammel Nets for
Sampling Freshwater Fishes",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "464",
day = "16",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090464",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/464",
abstract = "A multi-mesh trammel net has been developed and used
for sampling freshwater fishes. However, little is
known about the catch efficiency of the net. This
research investigated the catch efficiency of a
multi-mesh trammel net (nominal mesh size; 10.0, 30.0,
50.0, and 70.0 mm) for fish sampling in the Yangtze
River of China. Catch composition and factors affecting
catch per unit effort based on fish number (CPUE N),
weight (CPUE W), and species number (CPUE S) were
analyzed. The results showed that the net was capable
of capturing a wide range of fish sizes (total length,
5.5 to 121.0 cm) and species (n = 50). Increasing soak
time from 9.4 to 24.0 h resulted in a decrease in CPUE
N and CPUE W while a longer soak time increased CPUE S.
CPUE N, CPUE W, and CPUE S varied significantly with
fishing locations. The net provides a potential
complement to the current fish sampling techniques used
in freshwater ecosystems (e.g., large rivers, lakes,
and reservoirs). The findings of this research help to
improve our understanding of the catch efficiency of
the multi-mesh trammel net and provides insight into
better designs for gears and methods for sampling
diverse fish sizes and species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Muslim:2023:SSM,
author = "Ahmad Imam Muslim and Miho Fujimura and Tsuji Kazunari
and Muslim Salam",
title = "Small-Scale Marine Fishers' Possession of Fishing
Vessels and Their Impact on Net Income Levels: a Case
Study in \geoname{Takalar District, South Sulawesi
Province, Indonesia}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "463",
day = "15",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090463",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/463",
abstract = "Over the last two decades, the growth of the fisheries
sector in Indonesia has shown an increasing trend;
however, behind the rapid development of this sector,
the role of small-scale fisheries as one of the main
actors supporting the whole industry is often
neglected. They remain poor and continue to conduct
fishing activities traditionally. Therefore, this study
aims to describe the real situation of small-scale
fishers, analyzing the fishers' ownership of the boat
they use, analyzing their income level to reveal their
poverty status, and analyzing the factors affecting
their income. The regression analysis results indicate
that boat category, sea fish catch, fish selling price,
fixed costs, and variable costs have significant
effects on fishers' net incomes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Leite:2023:SUP,
author = "Tamara Leite and Daniel Mameri and Paulo Branco and
In{\^e}s Vieira and Margarida Oliveira and Jos{\'e}
Maria Santos",
title = "Swimming under Pressure: The Sub-Lethal Effects of a
Pesticide on the Behaviour of Native and Non-Native
Cypriniformes Fish",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "462",
day = "15",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090462",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/462",
abstract = "River ecosystems are exposed to a multitude of
stressors, including increasing pesticide run-off
driven by precipitation and irrigation. Pyrethroids are
the fourth major group of insecticides in use worldwide
and have extremely negative effects on aquatic fauna.
In this study, we aimed to assess the effects of an
acute 2 h sub-lethal exposure to different levels of
the pyrethroid esfenvalerate on the swimming behaviour
of two Cypriniformes species: the native Iberian barbel
(Luciobarbus bocagei) and the non-native invasive bleak
(Alburnus alburnus). The experimental set-up consisted
of previous exposure to three esfenvalerate
concentrations (control, 1.2 (low), and 2.0 (high)
{\mu}g/L) before being stocked in a
three-artificial-flume-channel mesocosm for behavioural
trials through direct observation. Monitored behaviours
included (i) routine activity, (ii) shoal cohesion, and
(iii) boldness. Significant differences in fish
behaviour were detected for the native species
(barbel), as individuals spent significantly more time
holding position (i.e., resting) in the control
(44.9\%) than in the high esfenvalerate concentration
(25.2\%). Concordantly, control barbels were also found
to perform more directional changes than the ones
exposed to high esfenvalerate concentrations.
Behavioural changes were also found for boldness,
measured by the proportion of fish attempts to
negotiate the upstream ramp, which were significantly
higher in the control (37.4\%) and in the high
concentration (41.5\%) compared to the low one
(21.1\%). Finally, regarding shoal cohesion of the
barbel, it was tighter in the control (81.3\%) than in
the low- (70.5\%) and high- (71.1\%) esfenvalerate
treatments. For the invasive bleak, there were no
significant differences in any of the behavioural
traits upon previous exposure to an increasing
esfenvalerate concentration. This experimental study
demonstrated that even short-term exposure to the
pyrethroid esfenvalerate was sufficient to alter the
behaviour of a native Cypriniformes fish species while
not affecting the non-native species. This may confer
greater competitive advantages to non-native fish
species in the context of global changes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Diaz:2023:EMB,
author = "Patricio A. D{\'\i}az and Gonzalo {\'A}lvarez",
title = "Effects of Microalgal Blooms on Aquaculture and
Fisheries",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "461",
day = "15",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090461",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/461",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tweedley:2023:SFM,
author = "James R. Tweedley and Clara Obreg{\'o}n and Sarah J.
Beukes and Neil R. Loneragan and Michael Hughes",
title = "Selecting from the Fisheries Managers' Tool-Box:
Recreational Fishers' Views of Stock Enhancement and
Other Management Options",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "460",
day = "14",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090460",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/460",
abstract = "As recreational fishers act relatively autonomously,
management relies heavily on voluntary compliance.
Therefore, understanding fishers' views on management
options can be beneficial. This study used a two-phase
approach of face-to-face interviews and subsequent
online questionnaires to evaluate recreational fishers'
salient views on issues affecting the Blue Swimmer Crab
(Portunus armatus) and Black Bream (Acanthopagrus
butcheri) fisheries in Western Australia as well as
current and potential management arrangements,
including stock enhancement. The strength and
heterogeneity of views were also determined. Minimum
size limits were mainly considered acceptable or very
acceptable, with restricting recreational fishing and
spatial closures the least supported management options
for both species, in addition to maximum size limits
for crabs. These views were not always consistent
across fishing locations for each species and among
types of crab fishers, indicating heterogeneity in
views. Stocking was the most acceptable of the
management measures not already utilised for crabs and
among the most popular for bream fisheries.
Recreational fishers of both species believed stock
enhancement could have strong positive outcomes for the
abundance of their target species and increase their
subsequent catches. They also recognized that some
negative outcomes, e.g., increased fishing pressure and
environmental issues, might occur but considered them
unlikely.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ge:2023:ACI,
author = "Guangyu Ge and Zhihong Liu and Tao Yu and Liqing Zhou
and Xiujun Sun and Zhuanzhuan Li and Yanxin Zheng",
title = "Aminotransferase Class {I} and {II} Gene Family in the
\geoname{Jinjiang} Oyster (\bioname{Crassostrea
ariakensis}): Genomewide Identification, Phylogenetic
Analysis and Expression Profiles after Salinity
Stress",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "459",
day = "14",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090459",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/459",
abstract = "Aminotransferases are enzymes found in living
organisms that catalyze transfer reactions between
amino acids and keto acids, crucial for amino acid
metabolism and synthesis. Aminotransferase classes I
and II play a vital role in regulating osmolarity,
protecting cells, and improving metabolic homeostasis
and cellular fitness. To investigate the
characteristics of the aminotransferase class I and II
gene family and their roles in osmotic pressure
regulation in the Jinjiang oyster (Crassostrea
ariakensis), the gene structure, chromosomal
localization, and phylogeny were characterized and the
genes' expression in the gill under high-salt stress
was analyzed. In this study, eighteen Aminotransferase
class I and II genes, including SPTLCa and SPTLCb, SPTa
and SPTb, ALAT2a and ALAT2b, KAT3a and KAT3b, and ASTa,
ASTb and ASTc, were identified. The physicochemical
properties of 11 family members were stable, with their
instability factors less than 40. Subcellular
localization prediction showed that aminotransferase
classes I and II were localized in the cytoplasm or
mitochondria. Chromosomal localization results showed
that the 18 aminotransferase class I and II genes were
located on eight chromosomes. All members of this gene
family had the Aminotran_1_2 structural domain which is
associated with osmotic pressure regulation by
adjusting the conversion reaction between amino acids
and keto acids. Most gene expressions showed an initial
increase followed by a decrease from 0 h to 12 h when
the oysters were challenged by acute stress using
artificial seawater with a salinity of 40. However, the
expression of CarAGT2 and CarKAT3b genes showed an
increased trend with increasing stress time. This study
systematically investigated the bioinformatics
characteristics of the aminotransferase class I and II
gene family in C. ariakensis and their role in osmotic
pressure regulation, which provides scientific data for
understanding the potential functions of these genes in
physiological adaptation, thereby expanding the
research on osmoregulation in bivalves.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:MBF,
author = "Jilong Wang and Peilun Li and Wei Liu and Wanqiao Lu
and Fujiang Tang",
title = "The Migratory Biology and Feeding Habits of
Downstream-Migrating Juvenile Chum Salmon
\bioname{Oncorhynchus keta} in the \geoname{Amur River
of Northeast China}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "458",
day = "14",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090458",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/458",
abstract = "The size of chum salmon juveniles is crucial to their
survival. In order to understand the population status
and migration patterns of juvenile chum salmon in the
waters of the Amur River in China, this study
investigated the status of juvenile chum salmon
resources and their basic biological characteristics in
the Amur River and the Ussuri River in China. The
results showed that the average catch per unit effort
(CPUE) of chum salmon in river margins was 0.140
ind{\cdot}10 {$-$3} m 3 for the Amur River and 0.255
ind{\cdot}10 {$-$3} m 3 for the Ussuri River. Chum
salmon migrate downstream, mainly in mid-May in the
Amur River and in early May in the Ussuri River, and no
fish was caught in the rivers after June. Most chum
salmon migrated when the water was between 10 and 14
{$^\circ $}C. The average FL (fork length) and BW (body
weight) of the Amur River samples were 37.1 {\pm} 2.9
mm and 0.42 {\pm} 0.09 g, respectively, while the
Ussuri River samples' FL and BW were 34.9 {\pm} 3.7 mm
and 0.36 {\pm} 0.08 g, respectively. The empty stomach
rate of the samples was zero, and the prey category of
the samples was composed of fish, aquatic insects,
copepods, and cladocerans, of which Ephemeroptera had
the largest percentage index of relative importance
(IRI \%), with a value of 58.45\%. The size of the
downstream-migrating juvenile chum salmon in this study
is similar to the size of those in some other rivers,
and the CPUE varies depending on the river
conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:EAG,
author = "Peilun Li and Jiacheng Liu and Tai Wang and Jilong
Wang",
title = "Estimates of the Age, Growth, and Mortality of
\bioname{Triplophysa scleroptera} ({Herzenstein}, 1888)
in the Upper Reaches of the \geoname{Yellow River,
China}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "457",
day = "13",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090457",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/457",
abstract = "To investigate the age composition, growth pattern,
mortality, and exploitation rate of Triplophysa
scleroptera in the upper reaches of the Yellow River,
we measured the total length (L) and body weight (W) of
347 individuals based on three sampling surveys from
2022 to 2023. The otoliths were used in this
investigation to determine age. The total length of the
collected samples ranged from 5.69 cm to 18.40 cm, body
weight ranged from 1.65 g to 50.22 g, and the age
ranged from 1 to 7 years old. The relationship of total
length and body weight was W=0.009L2.942 for the total
samples, and the growth pattern observed in the study
belongs to the isometric type. The von Bertalanffy
growth equation revealed that the fish had an
asymptotic total length L {\infty} of 37.536 cm, and
the growth coefficient K was 0.064 yr {$-$1}. Total
instantaneous mortality rate (Z) of total samples
calculated by the age-based catch curve method was
1.092 yr {$-$1}. The average instantaneous rate of the
natural mortality (M), estimated by three different
methods, for the total samples was 0.237 yr {$-$1}. The
instantaneous rate of fishing mortality (F) for the
total samples was calculated as 0.855 yr {$-$1}, and
the exploitation rate (E) was determined as 0.783. As a
whole, compared with other Triplophysa fishes, the
growth rate of T. scleroptera in the upper reaches of
the Yellow River is relatively slow, and the population
of T. scleroptera has been overexploited. It is crucial
to establish some effective management strategies to
protect this species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ma:2023:TSA,
author = "Yanwen Ma and Yingying Ye and Ronghui Yao and Pengzhi
Qi and Jiji Li",
title = "Transcriptome Sequencing Analysis of Sex-Related Genes
in the Gonads of \bioname{Mytilus unguiculatus}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "456",
day = "11",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090456",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/456",
abstract = "In the cultivation of Mytilus unguiculatus, the
broodstock are shade-dried to stimulate sperm and egg
production. To identify the functional genes affecting
gonad development in M. unguiculatus, the transcriptome
of gonads in mussels stimulated by shade-drying and
those not stimulated were compared. Differential gene
expression analysis revealed that there were 22
differentially expressed genes (DEGs) in the testis and
70 DEGs in the ovary among the experimental groups.
KEGG enrichment analysis identified a total of 11
pathways that might be related to environmental stimuli
affecting gonadal development. Nicotinic acetylcholine
receptors (AChRs), the cholecystokinin A receptor
(CCKAR), hypocretin (orexin) receptor 2 (HCRTR2), and
gamma-aminobutyric acid type B receptor (GABBR) were
highly expressed in the neuroactive ligand-receptor
interaction pathway, indicating that these genes might
be involved in the transduction of environmental
information that stimulates gonadal development.
Meanwhile, nuclear receptor co-repressor 2 (NCoR2) was
highly expressed in the notch signaling pathway,
indicating that NCoR2 might be involved in the
regulation of gonad development. To validate the
transcriptome data, we selected five DEGs in the KEGG
signaling pathway, including AChRs, CCKAR, HCRTR2,
GABAB, and NCoR2, for real-time quantitative PCR
(RT-qPCR), which produced results consistent with the
RNA-Seq data. The transcriptome analysis and gene
pathway identification in this study have enhanced our
comprehension of the reproductive mechanisms in M.
unguiculatus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Imsland:2023:CFD,
author = "Albert Kjartan Dagbjartarson Imsland and Jon Petter
Berg and Velimir Nola and Lena Geitung and Tina
Oldham",
title = "Cleaner Fish Do Not Impact the Pigmentation of Salmon
Lice (\bioname{Lepeophtheirus salmonis}) in Commercial
Aquaculture Cages",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "455",
day = "10",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090455",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/455",
abstract = "Salmon lice are one of the biggest challenges to
sustainable salmonid aquaculture. The species display
high evolutionary potential, which is evident by its
development of resistance to numerous chemical
compounds used for delousing. In response to this,
salmon farms now use non-chemical delousing methods to
minimize the damage done by salmon lice, including
heavy reliance on cleaner fish. Anecdotal reports from
farmers and fish health personnel in areas where
cleaner fish are used have suggested that salmon lice
are becoming less pigmented, potentially making them
harder for cleaner fish to visually detect. This
experiment investigated changes in the pigmentation of
salmon lice in relation to the use of cleaner fish,
louse stage and sex, temperature, preferred salmon
swimming depth, daylength, and salinity. Salmon lice
were sampled from snorkel cages on a commercial salmon
farm where three cages were stocked with farmed
lumpfish and ballan wrasse, and three cages were
without cleaner fish. Water temperature, salinity, and
depth were recorded using a conductivity, temperature,
and depth recorder. Pigmentation was measured via
photographic analysis of individual lice. Although
louse pigmentation varied considerably throughout the
experiment, using cleaner fish throughout a single
production cycle did not reduce average louse
pigmentation compared to control cages. On average,
male lice were significantly darker pigmented than
females, but otherwise there were no patterns in louse
pigmentation in relation to life stage, salinity,
temperature, or daylength. Salmon lice exhibit a high
degree of evolvability and have become resistant to
every chemical removal treatment developed thus far.
The present data suggest that, with the densities and
species of cleaner fish commonly used in commercial
salmon production, there is not strong directional
selection on louse pigmentation. Lice, at least with
regard to visual appearance, are not likely to adapt in
a way which reduces cleaner fish efficacy anytime
soon.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pan:2023:EAM,
author = "Tingshuang Pan and Min Yang and He Jiang and Tong Li
and Guoqing Duan and Jun Ling and Qian Gao",
title = "Effect of \bioname{Astragalus membranaceus} on
Transcriptome and Survival of Hybrid Yellow Catfish
(\bioname{Pseudobagrus vachellii} [male sign] $ \times
$ \bioname{Tachysurus fulvidraco} [female sign]) in
Response to \bioname{Aeromonas hydrophila} Challenge",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "454",
day = "10",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090454",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/454",
abstract = "Intensive culturing of hybrid yellow catfish
(Pseudobagrus vachellii {[male-sign]} $ \times $
Tachysurus fulvidraco {[female-sign]}) has increased
their mortality. Astragalus membranaceus has been used
as an immune stimulant and antioxidant in fish for
several years. A. membranaceus was decocted and mixed
with the diet. After feeding for 28 d, the hybrid
yellow catfish were challenged with Aeromonas
hydrophila. To better understand the function of A.
membranaceus in the defense of hybrid yellow catfish
against A. hydrophila, we analyzed the spleen
transcriptome data and relative percentage survival
(RPS). There were 396 differentially expressed genes
(DEGs) between the A. membranaceus and control groups
at 24 h after A. hydrophila stimulation, including 263
upregulated and 133 downregulated DEGs. A significant
enrichment of DEGs was found in the A. membranaceus
group when the GO enrichment terms in the spleen were
analyzed. The qRTPCRresults for the five upregulated
and two downregulated DEGs from the spleen, intestine,
and liver were consistent with the transcriptome data.
The relative percentage survival of A. membranaceus was
85.71\% after the fish were challenged with A.
hydrophila.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Selvaraj:2023:SSF,
author = "John Josephraj Selvaraj and Leidy Viviana Rosero-Henao
and Maria Alejandra Cifuentes-Ossa",
title = "Small-Scale Fisheries in the \geoname{Colombian
Pacific}: Understanding the Impact of Climate Change on
Fishermen's Livelihoods",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "453",
day = "09",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090453",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/453",
abstract = "While few have contemplated the potential impacts of
climate change on small-scale fisheries and fishermen
in the Colombian Pacific, understanding these effects
is crucial for devising effective adaptation
strategies. This study presents the outcomes of a
vulnerability assessment related to climate change for
fishermen in the Colombian South Pacific. The
assessment employed a multivariable model that
considered the sensitivity of marine and coastal
ecosystems that support fishing resources alongside
fishermen's adaptability to changes in the biophysical
environment. Socioeconomic factors were also
scrutinized. The study revealed that 80\% of the
surveyed fishermen are at a medium to high level of
vulnerability due to changes in the distribution of key
commercial species, which influences the productivity
of their traditional fishing sites. Their vulnerability
is primarily attributed to low usage of fishing
technologies, the boat engine's limited propulsion
capacity, and a lack of diversity and sustainability in
fishing gear. These elements increase their reliance on
fishing sites close to the coast (approximately 3 km to
5 km from the shoreline), resulting in a low adaptive
capacity for the fishermen. The dominant factor driving
the sensitivity component was the economic significance
of the fishing gear and the fact that the gear could be
more diverse and durable. These findings provide a
critical scientific basis for assessing the fishing
sector's vulnerability to climate change and informing
future collaborative decision-making in managing
small-scale fisheries in the Colombian South Pacific
Ocean.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Siamujompa:2023:IBP,
author = "Mazuba Siamujompa and Kunda Ndashe and Frederick
Chitonga Zulu and Chanda Chitala and Mwansa M. Songe
and Katendi Changula and Ladslav Moonga and Emmanuel
Shamulai Kabwali and Stephen Reichley and Bernard
Mudenda Hang'ombe",
title = "An Investigation of Bacterial Pathogens Associated
with Diseased \geoname{Nile} Tilapia in Small-Scale
Cage Culture Farms on \geoname{Lake Kariba, Siavonga,
Zambia}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "452",
day = "08",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090452",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/452",
abstract = "This study investigated disease outbreaks in farmed
Oreochromis niloticus (Nile tilapia) in Siavonga among
small-scale cage culture farms on Lake Kariba in order
to establish bacterial etiological agents associated
with fish mortality and to determine their antibiotic
susceptibility. A total of 300 fish samples from 11
farms were aseptically collected and bacteria were
isolated from the kidney, liver, brain, and spleen. The
isolates were identified using their morphological
characteristics and conventional biochemical tests. The
antibiotic susceptibility of selected bacteria was
determined by the Kirby--Bauer disc diffusion method.
The following well-known fish pathogens were identified
at a prevalence of Aeromonas spp. (13\%), Pseudomonas
spp. (10.3\%), Micrococcus spp. (9.7\%), Klebsiella
spp. (8.7\%), Lactococcus spp. (7.3\%), Streptococcus
spp. (7.0\%), and Acinetobacter spp. (7.0\%). All the
isolates tested were susceptible to doxycycline, and
complete resistance to ciprofloxacin, co-trimoxazole,
and cephalothin was recorded in the Bacillus spp. The
observed resistance could be attributed to bacteria
from terrestrial sources as fish farmers do not
administer antibiotics to fish. To our knowledge, this
is the first study to establish the occurrence of
several bacterial species infecting tilapia in Zambia
and the first to determine the antibiotic
susceptibility of fish bacteria among small-scale farms
on Lake Kariba. The current study provides baseline
information for future reference and fish disease
management on Lake Kariba and in Zambia.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:YRS,
author = "Jun Yu Chen and Chaoshu Zeng and Jennifer M.
Cobcroft",
title = "Year-Round Spawning, Filial Cannibalism, and Embryonic
and Larval Development of the Coral Reef Fish Orchid
Dottyback, \bioname{Pseudochromis fridmani}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "451",
day = "08",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090451",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/451",
abstract = "Aquaculture of marine ornamental fish could
potentially reduce the fishing pressure on wild stocks
by the global aquarium trade, but its expansion is
often constrained by the limited understanding on the
biology and early life history of candidate species.
The orchid dottyback Pseudochromis fridmani is a
valuable and popular marine ornamental fish, but
scientific reports on its baseline biology, especially
the egg filial cannibalistic behaviour, are scarce. The
present study documented key aspects of reproductive
biology and early ontogeny of P. fridmani in captivity,
including reproductive behaviour, patterns of spawning
and filial cannibalism throughout a 12-month period by
seven pairs of brood fish, as well as the embryonic and
larval development. The results showed that the captive
spawning of the broodstock pairs generally occurred
every 5--11 days, most commonly every 6--8 days,
throughout a year. Despite feeding the brood fish to
satiation throughout the data collection period, the
average monthly rate of egg filial cannibalism reached
55 {\pm} 37\%, but the cannibalism frequency appeared
to be pair-specific. The egg incubation duration was
approximately 96 h at 27 {\pm} 1 {$^\circ $}C, and the
development of embryos from cleavage, blastula,
gastrula, and segmentation to the pharyngula stage is
herein described in detail. The newly hatched P.
fridmani larvae possessed important structures and
organs for first feeding, including pigmented eyes,
developed jaws, and a straight-tube gut. The 0 to 14
days post-hatching (DPH) period appeared to be an
important larval stage, as P. fridmani larvae were
observed to complete major changes in morphology, gut
development, and phototactic behaviour by 14 DPH. Under
the culture conditions of this study, the earliest
transition to the juvenile stage was observed on 31
DPH, and the majority of fish became juveniles by 56
DPH. The results of this study inform baseline
aquaculture production protocols and direct future
research, particularly to reduce filial cannibalism
through broodstock management and to improve larval
culture through supporting the early ontogenetic
development of P. fridmani. Additionally, these
findings form a foundation for further studying the
biology and ecology of P. fridmani in the wild.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Esposito:2023:PHF,
author = "Ana{\"\i}s Esposito and Jos{\'e}phine Foata and Yann
Quilichini",
title = "Parasitic Helminths and Freshwater Fish Introduction
in \geoname{Europe}: a Systematic Review of Dynamic
Interactions",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "450",
day = "07",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090450",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/450",
abstract = "The introduction of non-native freshwater fish is a
primary cause of aquatic biodiversity loss at global
scale. Such introductions have a severe impact on
freshwater ecosystems in terms of competition,
predation, habitat alteration, genetic pollution and
transmission of diseases and parasites. A systematic
review was conducted on the helminths parasites of
freshwater fish in the context of species introduction
and a total of 199 publications were retrieved between
1969 and November 2022. Several scenarios may arise
when a new fish species arrive in a recipient area.
Non-native fish hosts can co-introduce their parasites
without transmitting them to native fish (e.g., the
case of North American Centrarchidae and their
Monogenea parasites). Another possible outcome is the
transfer of these parasites to the native fish fauna
(spillover, e.g., the cases of the Nematoda
Anguillicola crassus Kuwahara, Niimi \& Itagaki, 1974
and the Monogenea Gyrodactylus salaris Malmberg, 1957).
Reciprocally, non-native fish hosts may acquire
parasites in their new distribution range whether these
parasites are native or were previously introduced
(e.g., the cases of Ponto-Caspian Gobiidae and the
Chinese sleeper Perccottus glenii Dybowski, 1877).
Acquired parasites can then be spilled back to the
native fauna. This phenomenon is of particular interest
when non-native fish hosts influence the dynamics of
zoonotic parasites.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yuan:2023:CTP,
author = "Hang Yuan and Naijie Hu and Yudong Zheng and Cuihong
Hou and Beiping Tan and Lili Shi and Shuang Zhang",
title = "A Comparison of Three Protein Sources Used in
Medium-Sized \bioname{Litopenaeus vannamei}: Effects on
Growth, Immunity, Intestinal Digestive Enzyme Activity,
and Microbiota Structure",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "449",
day = "06",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090449",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/449",
abstract = "The type of protein source in diets has many effects
on shrimp. In this study, Litopenaeus vannamei with an
initial body weight of 3.68 {\pm} 0.002 g were fed for
8 weeks on three experimental diets (isoproteic:
41.00\%; isolipidic: 7.61\%) that were formulated using
fish meal (FM), Chlorella sorokiniana (CHL), and
Clostridium autoethanogenum protein (CAP) as the
primary protein sources, respectively. This study
examined the growth, non-specific immunity, intestinal
digestion, and microbiota of L. vannamei after the
feeding experiment concluded. Compared to the FM group,
the findings indicate that the weight gain rate (WGR),
specific growth rate (SGR), and protein efficiency
ratio (PER) of L. vannamei were notably enhanced via
dietary CAP. The CHL group exhibited the highest levels
of catalase (CAT), phenoloxidase (PO), and superoxide
dismutase (SOD) activities in the gills of L. vannamei,
whereas the FM group had the lowest levels. Conversely,
the malondialdehyde (MDA) content showed the opposite
trend. Both dietary CHL and CAP promoted the digestive
enzyme activities of L. vannamei, with dietary CAP
having a more pronounced promotional effect. An
analysis of alpha diversity indicated that the
consumption of dietary CHL substantially enhanced the
abundance and diversity of microbiota in the intestinal
tract of L. vannamei. Furthermore, the dietary CHL
significantly increased the colonization of
immune-associated beneficial bacteria and inhibited the
colonization of pathogenic bacteria in the intestinal
tract of L. vannamei, whereas dietary CAP mainly
increased the colonization of growth-associated
beneficial bacteria. Functional predictions showed that
different dietary protein sources affect various
metabolic activities and signaling pathways of L.
vannamei, and some functions, including signal
transduction, cell motility, and the immune system,
were significantly enhanced in the CHL group. In
summary, both dietary CHL and dietary CAP promoted
growth and immunity in L. vannamei compared to dietary
FM. The results of this study could be helpful for the
sustainable development of shrimp farming.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:EDF,
author = "Qin Zhang and Qiuyue Yang and Mengjie Guo and Fanghui
Li and Meilan Qin and Yi Xie and Jian Xu and Yongqiang
Liu and Tong Tong",
title = "The Effects of Dietary Fermented Soybean Meal
Supplementation on the Growth, Antioxidation, Immunity,
and \gene{mTOR} Signaling Pathway of Juvenile {Coho}
Salmon (\bioname{Oncorhynchus kisutch})",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "448",
day = "05",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090448",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/448",
abstract = "This experiment aims to investigate the effects of
partial replacement of fish meal (FM) by soybean meal
(SBM) and/or fermented soybean meal (FSBM) on the
growth, serum biochemistry, digestion, antioxidation,
immunity, and mTOR signaling pathway of juvenile coho
salmon (Oncorhynchus kisutch). Four iso-nitrogen and
iso-lipid diets were designed and fed to four groups of
juvenile coho salmon (152.25 {\pm} 2.96 g) in
triplicate for 10 weeks. The four diets were the G0
diet (control group, containing 28\% FM protein), the
G1 diet (containing 10\% SBM protein and 18\% FM
protein), the G2 diet (containing 5\% SBM protein, 5\%
FSBM protein, and 18\% FM protein), and the G3 diet
(containing 10\% FSBM protein and 18\% FM protein). It
was found that compared with the G0 diet (control
group), the activities of catalase (CAT), superoxide
dismutase (SOD), pepsin, trypsin, {\alpha}-amylase, and
lipase, and the expression levels of mammalian target
of rapamycin (mtor), protein kinase B (akt),
phosphatidylinositol 3-kinase (pi3k), plant ribosome
S6K protein kinase (s6ks), and lysozyme (lyz) genes, of
juvenile coho salmon fed the G3 diet increased
significantly (p {$<$} 0.05), and the expression levels
of tumor necrosis factor (tnf-{\alpha}),
interleukin-1{\beta} (il-1{\beta}), and interleukin-6
(il-6) genes of juvenile coho salmon fed the G3 diet
decreased significantly (p {$<$} 0.05). However, there
were no significant differences in growth, muscle
composition, and serum biochemistry of juvenile coho
salmon fed the G3 diet compared with the G0 diet (p
{$>$} 0.05). In conclusion, replacing 10\% FM protein
with FSBM protein could improve the digestion,
antioxidation, immunity, and mTOR signaling pathway of
juvenile coho salmon.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liontakis:2023:EVE,
author = "Angelos Liontakis and Vassiliki Vassilopoulou",
title = "An Economic View on the Effects of Invasive
Rabbitfishes Based on Fishers' Perspectives: The Case
of the Parrotfish M{\'e}tier in the \geoname{South
Ionian Sea}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "447",
day = "04",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090447",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/447",
abstract = "The effect of thermophilic species invasion in the
eastern Mediterranean is well-noted in the literature.
During the last decade in western Greek waters,
small-scale fisheries (SSF) catches exhibit increased
relative contribution of rabbitfishes, which have
originated from the Red Sea and inhabited the country's
southeastern part since the Second World War. This
study has used quantitative and qualitative information
to assess the short-term economic effect of the
rabbitfishes' expansion in the SSF m{\'e}tier targeting
the indigenous thermophilic parrotfish in the South
Ionian Sea. The short-term micro-level negative
economic effects of the rabbitfishes' invasion and the
corresponding adverse effects on fishers' economic
wealth have been estimated. The results indicate
attitudinal differences among fishers, although their
local ecological knowledge may enable them to avoid
rabbitfishes' hot spots and mitigate the respective
economic losses, that particularly for smaller SSF
vessels already seem to be quite important. As climate
warming may further facilitate the expansion of
rabbitfishes, outcompeting parrotfish, the latter
m{\'e}tier may eventually become economically
unsustainable. Fine-scale analysis at the m{\'e}tier
level enables addressing the specificities of
social-ecological systems and can contribute to
informed and more effective decision making related to
SSF, which is an important building block of
Mediterranean coastal communities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dance:2023:LCC,
author = "Michael A. Dance and Mitchell S. Lovell",
title = "Lipid Correction for Carbon Stable Isotope Analysis of
Yellowfin Tuna",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "446",
day = "01",
month = sep,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090446",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/446",
abstract = "Carbon stable isotopes ({\delta} 13 C) are widely used
in ecological studies to understand diet, food web
dynamics, and movements of marine fishes. Still,
{\delta} 13 C is influenced by lipid content and often
requires chemical extraction or mathematical
correction. Here, we developed a species-specific
mathematical lipid correction for white muscle tissue
of yellowfin tuna (Thunnus albacares), a highly
migratory finfish of considerable economic and
ecological value. Lipid extraction was conducted on
yellowfin tuna white muscle tissue (C:N range:
2.96--6.49), and both linear and non-linear lipid
correction models for {\delta} 13 C were fitted and
assessed. Lipid extraction increased {\delta} 13 C, and
to a lesser extent, {\delta} 15 N values in yellowfin
tuna white muscle tissue, but had no effect on {\delta}
34 S. Both non-linear models provided better fits to
the data than the linear model, suggesting an
asymptotic relationship between C:N and
\SGMLentity{8710}{\delta} 13 C. Results support the
growing body of evidence that C:N ratios can be used to
predict lipid corrected {\delta} 13 C and highlight the
value of mathematical correction approaches. We provide
species-specific parameter estimates that can be used
for lipid correction of white muscle tissue for
{\delta} 13 C analysis in yellowfin tuna and similar
species for which species-specific models have yet to
be developed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Madariaga-Mendoza:2023:EIM,
author = "Diana Madariaga-Mendoza and Jos{\'e} Marrugo-Negrete
and V{\'\i}ctor Atencio-Garc{\'\i}a",
title = "Effect of Inorganic Mercury on Semen Quality, Embryo
and Larval Development of Bocachico
\bioname{Prochilodus magdalenae}",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "445",
day = "31",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090445",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/445",
abstract = "The study aimed to evaluate the effect of inorganic
mercury (iHg) on the sperm quality, embryos, and larvae
of Prochilodus magdalenae. Two experiments were carried
out. In experiment 1, semen was activated with
contaminated water at different iHg concentrations
(0.0, 25, 50, 100 {\SGMLmicro}g/L) and sperm kinetics
were analyzed with a computer-assisted semen analysis
(CASA) system. In the second trial, 2 g of oocytes were
fertilized with 50 {\SGMLmicro}L of milt, activated
with contaminated water at different iHg concentrations
(0.0, 25, 50, 100 {\SGMLmicro}g/L), and maintained that
way during the first hour of incubation. Samples were
taken every 3--4 h until hatching to identify embryo
deformations. Fertilization and hatching rates were
estimated. The survival of newly hatched larvae (1 day)
and larvae eight days post-hatching (dph) was also
assessed. The results show that the quality parameters
of semen treated with contaminated water at different
iHg concentrations (25, 50, 100 {\SGMLmicro}g/L) were
altered and showed significant differences (p {$<$}
0.05) from the control group (0 {\SGMLmicro}g/L). Total
motility decreased (9.8--13.2\%) and the amount of
immotile sperm increased (3-fold) relative to values in
the control group. A significant increase in oocyte
diameter and a decrease in fertilization and hatching
rates were observed with the higher iHg concentrations
(50--100 {\SGMLmicro}g/L). Embryo deformations
({$>$40}\%) such as scoliosis, lordosis, and
microcephaly were observed, as well as premature
hatchings. The larval survival at 1 dph was reduced
between 25\% (25 {\SGMLmicro}g/L) and 97.2\% (100
{\SGMLmicro}g/L) relative to that in the control group.
The results suggest that bocachico semen, embryos, and
larvae are sensitive to contamination with iHg from 25
{\SGMLmicro}g/L.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bischoff:2023:DFA,
author = "Adrian A. Bischoff and Melanie Kubitz and Laura
Ballesteros-Redondo and Marcus St{\"u}eken and Tobias
Rapp and Patrick Fink and Wilhelm Hagen and Harry
Wilhelm Palm",
title = "Dynamics of Fatty Acids in Pikeperch (\bioname{Sander
lucioperca}) Larvae and Juveniles during Early Rearing
and Weaning in a Commercial {RAS} --- Implications for
Dietary Refinement",
journal = j-FISHES,
volume = "8",
number = "9",
pages = "444",
day = "31",
month = aug,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8090444",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:13 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/9/444",
abstract = "The aquaculture production of pikeperch has reached
commercial scale in a number of European countries, but
the high mortality of early life cycle stages and minor
understanding of nutritional requirements are still
major bottlenecks. To investigate the fate of fatty
acids during early development, weaning and rearing,
pikeperch larvae and juveniles from a commercial
recirculating aquaculture system (RAS) were sampled
over 2 months for morphometric data, as well as fatty
acid composition, with a total of 6 sampling days, with
four to five replicates per sampling day and between 1
and 25 pikeperch larvae per individual sample,
depending on larval biomass. The biomass of sampled
pikeperch larvae varied from 0.1 to 420 mg (dry mass
DM), depending on the age of the larvae, and the
initial length of the pikeperch larvae was about 4.5
mm. Our data confirm that, accompanied by an
exponential increase in dry mass, total fatty acids
(TFAs) in larval tissues increased with the beginning
of exogenous feed uptake and were depleted between days
13 and 25 post hatch, most likely associated with the
weaning and metamorphosis of the larvae. We conclude
that all fatty acid classes may serve as metabolic fuel
during metamorphosis, but the ultimate fatty acid
composition is strongly impacted by the available feed.
The chosen diet probably caused a lack of
alpha-linolenic (18:3n-3; ALA) and docosahexaenoic acid
(22:6n-3; DHA) during larval development and a shortage
of vaccenic (18:1n-7), alpha-linolenic (18:3n-3; ALA)
and arachidonic acid (20:4n-6; ARA) in juvenile
pikeperch. This led to low DHA/EPA ratios 13 days post
hatch, a high EPA/ARA ratio at days 41 and 56 post
hatch and a fluctuating ratio of alpha-linolenic acid
to linoleic acid (18:2n-6; LA). A temporary lack of
essential fatty acids can cause dysfunctions and
eventually mortalities in pikeperch larvae and
juveniles. Despite high larval growth rates, the
biochemical composition of the first fed Artemia and
microdiets was most likely not sufficient and in need
of improvement. We suggest that deficiencies must be
compensated, e.g., through the substitution of the
offered Artemia with more suitable live feed organisms,
such as freshwater rotifers, and the enrichment of
current microdiets in order to prevent high mortalities
during pikeperch rearing and weaning.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ziebell:2023:HUT,
author = "Ann-Christin Ziebell and Maite L. Vogel and Niklas
Kjell Ratajczak and Bert W. Hoeksema",
title = "Habitat Use of Two Coral-Associated Cryptobenthic
Gobiid Fishes (Family: {Gobiidae}) in the
\geoname{Southern Caribbean}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "531",
day = "23",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100531",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/531",
abstract = "Cryptobenthic fishes make up more than half of coral
reef fish fauna and contribute greatly to coral reef
trophodynamics and diversity. Because of their small
size, they are easily overlooked and understudied. Some
of them use corals as their habitat, but this
association is not well understood. In the Caribbean,
two common cryptobenthic gobies, Elacatinus evelynae
and Coryphopterus lipernes, are usually observed
residing on corals. In order to compare their habitat
use, we investigated their distributions on a range of
scleractinian host-coral species at three different
depths (5, 10, and 15 m) at Cura{\c{c}}ao, southern
Caribbean. The numbers of both species were relatively
low at 5 m. Furthermore, we investigated the
relationship between fish size and depth and found that
adult E. evelynae individuals were most common at 5 m
depth and juveniles at 15 m depth. Novel host corals
were found for both fish species. Taking host size into
account, the gobies were most abundant on large coral
colonies of two host species: E. evelynae on both
Colpophyllia natans and Montastraea cavernosa, and C.
lipernes only on C. natans. In summary, depth, host
species, and host-colony size were found to be
environmental factors that may determine the occurrence
of both fish species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fei:2023:ESF,
author = "Yingjie Fei and Shenglong Yang and Mengya Huang and
Xiaomei Wu and Zhenzhen Yang and Jiangyue Zhao and
Fenghua Tang and Wei Fan and Sanling Yuan",
title = "Evaluating Suitability of Fishing Areas for
Squid-Jigging Vessels in the \geoname{Northwest Pacific
Ocean} Derived from {AIS} Data",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "530",
day = "23",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100530",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/530",
abstract = "Understanding the spatial distribution of fishing
activity and suitable fishing areas is important for
improving sustainable fisheries management and
protecting vulnerable fish stocks. To identify
climate-related habitat changes and variations in the
distribution of fishing activity for squid-jigging
vessels in the Northwest Pacific Ocean, two types
(weighted arithmetic mean method, weighted-AMM;
weighted geometric mean method, weighted-GMM) of
habitat suitability index (HSI) models were developed
in this study with marine environmental data at
different depths. The boosted regression tree (BRT)
model was adopted to access the monthly important
environmental variables and the relative influence of
the corresponding variables. The results showed that
the weighted-AMM has better prediction performance than
the weighted-GMM. The suitable fishing areas showed
significant seasonal changes in both spatial location
and coverage area. The hotspot map showed that the
suitable fishing area for squid-jigging vessels was
located in the scope of 42 {\SGMLcompfn} N{\sim}44
{\SGMLcompfn} N, 155 {\SGMLcompfn} E{\sim}170
{\SGMLcompfn} E throughout the year during
2012{\sim}2019, which suggests that high squid-jigging
fishing pressure should be given more attention in
fishery management. The HSI model also had good
prediction performance for the fishery data of Chinese
companies, except for June and July. Additionally,
fishing efforts could be used as alternative data for
fishery research. The study has also suggested that
fishery data are restricted by spatial and temporal
distribution and fishing experience, which probably
biases the results of the research.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:EAC,
author = "Ying Zhang and Meng-Fei Li and Xiao-Han Fang",
title = "Efficiency Analysis of \geoname{China} Deep-Sea Cage
Aquaculture Based on the {SBM--Malmquist} Model",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "529",
day = "23",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100529",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/529",
abstract = "Deep-sea cage aquaculture (DSCA) is an important way
to expand new space for marine aquaculture, promote the
transformation and upgrade of the fishery industry, and
optimize the structure of marine aquaculture. Using the
panel data of DSCA in China's coastal areas from 2013
to 2021, this study constructs the SBM--Malmquist model
to measure the DSCA production efficiency and analyzes
its total factor productivity. The results show that
the overall DSCA production efficiency exhibited an
increasing trend in spite of a sharp decline in 2019.
The efficiency exhibited regional differences, being
the strongest in the Bohai Sea region, followed by in
the Yellow Sea, the South China Sea, and the East China
Sea regions. The overall total factor productivity
remained generally stable, although a large fluctuation
occurred between 2019 and 2021. Both pure technological
efficiency and scale efficiency promoted the total
factor productivity in 2019--2021, while the efficiency
of technological changes in societal aspects declined.
This study shows that the DSCA production efficiency is
significantly influenced by input factors such as labor
and capital investment. In addition, natural disasters
inhibit the improvement of the production efficiency to
some extent.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:MAP,
author = "Yu Zhang and Lu Qi and Fengping Li and Yi Yang and
Zhifeng Gu and Chunsheng Liu and Qi Li and Aimin Wang",
title = "Mitogenomic Analysis of {Pterioidea} ({Bivalvia:
Pteriomorphia}): Insights into the Evolution of the
Gene Rearrangements",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "528",
day = "23",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100528",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/528",
abstract = "The complete mitogenomes of Pinctada albina and
Pinctada margaritifera were sequenced in this study,
with sizes of 23,841 bp and 15,556 bp, respectively.
The mitochondrial genome analysis of eight Pterioidea
species indicated the existence of gene rearrangements
within the superfamily. The ATP8 gene was not detected
in the two new mitogenomes, and rrnS was found to be
duplicated in P. albina 's mitogenome. The
reconstructed phylogeny based on mitogenomes strongly
supported the monophyly of Pterioidea and provided
robust statistical evidence of the phylogenetic
relationships within Pteriomorphia. The analysis of the
mitochondrial gene order revealed that of P.
margaritifera to be the same as the ancestral order of
Pterioidea. The gene orders of the Pterioidea species
were mapped to the phylogenetic tree, and the gene
rearrangement events were inferred. These results
provide important insights that will support future
research, such as studies extending the evolutionary
patterns of the gene order from P. margaritifera to
other species and determining the evolutionary status
of Pterioidea within the infraclass Pteriomorphia.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sintori:2023:IAA,
author = "Alexandra Sintori and Vasilia Konstantidelli and
Angelos Liontakis and Stamatis Mantziaris and Irene
Tzouramani",
title = "Is It All about Profit? \geoname{Greek} Fishers'
Motives and Objective Profiles",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "527",
day = "22",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100527",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/527",
abstract = "Fishing contributes significantly to food security and
offers employment and income to many coastal
communities worldwide. On the other hand, it has been
recognized as a dangerous and demanding activity that
involves high economic risk and uncertainty as well as
outdoor work and interactions with nature, which makes
fishing more of a way of life rather than just a way to
make a living. Non-monetary attributes of fishing have
been reported to affect fishers' decision-making and
management and can explain the resilience and
sustainability of the sector, even in difficult
economic circumstances that hinder profitability. This
study uses multivariate statistical analysis to
identify profiles of Greek fishers according to their
motives and objectives and to link them to the main
demographic and socioeconomic characteristics. Overall,
five fisher profiles are identified, including ``profit
maximizers'', ``business-oriented'' and ``subsistence''
fishers. Our analysis indicates that all fishers value
the non-monetary attributes of the activity higher than
monetary values like profit, though large-scale fishers
seem to be more business-oriented than small-scale
fishers. The identification and understanding of the
objective profiles provide useful insights to
researchers, advisors and policymakers regarding
fishers' decision-making and can ultimately lead to
more efficient policy design and fisheries
management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Asencio-Alcudia:2023:SPR,
author = "Gloria Gertrudys Asencio-Alcudia and Cesar Antonio
Sep{\'u}lveda-Quiroz and Juan Carlos P{\'e}rez-Urbiola
and Mar{\'\i}a del Carmen Rodr{\'\i}guez-Jaramillo and
Andressa Teles and Joan Sebasti{\'a}n Salas-Leiva and
Rafael Mart{\'\i}nez-Garc{\'\i}a and Luis Daniel
Jim{\'e}nez-Mart{\'\i}nez and Mario Galaviz and Dariel
Tovar-Ram{\'\i}rez and Carlos Alfonso
Alvarez-Gonz{\'a}lez",
title = "Stress-Protective Role of Dietary $ \alpha
$-Tocopherol Supplementation in Longfin Yellowtail
(\bioname{Seriola rivoliana}) Juveniles",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "526",
day = "22",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100526",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/526",
abstract = "Aquaculture practices expose fish to several factors
that may generate stress, modifying the balance between
the production of reactive oxygen species (ROS) and the
activity of antioxidant defenses that induce cell
damage. Alpha-tocopherol (VE) improves the antioxidant
capacity against ROS production in fish. A 50-day trial
with longfin yellowtail (Seriola rivoliana) juveniles
was conducted to compare the dietary supplementation of
500 mg/kg of VE against a control diet without VE
supplementation on growth, lymphoid tissue enzymatic
activity, immune-system-related gene expression, and
the histology of the liver and spleen. Growth, weight
gain, specific growth rate, feed conversion rate, and
survival did not show significant differences (p {$>$}
0.05) among treatments. Fish fed with an
{\alpha}-tocopherol-enriched diet showed a higher
enzymatic activity of superoxide dismutase in the liver
(p {$<$} 0.05) and a lower percentage of
melanomacrophage coverage area in the lymphoid organs
(p {$<$} 0.05). Overexpression was observed of MyD88
and il-10 in the spleen, and il-1b in the liver in fish
fed 500 mg/kg of VE, as well as overexpression of
Toll-like 3 in the head kidney, spleen, and liver in
fish fed the control diet. Dietary supplementation with
VE reduces the effects of oxidative stress and improves
lymphoid tissue defense and immune-related gene
expression in S. rivoliana.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:UST,
author = "Ran Xu and Xiaoming Yang and Siquan Tian",
title = "Use of Space-Time Cube Model and Spatiotemporal Hot
Spot Analyses in Fisheries --- a Case Study of Tuna
Purse Seine",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "525",
day = "21",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100525",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/525",
abstract = "Katsuwonus pelamis, or skipjack, is a vital resource
in purse seine fishing across the Central and Western
Pacific. Identifying skipjack distribution hotspots and
coldspots is crucial for effective resource management,
but the dynamic nature of fish behavior means these
spots are not constant. We used Chinese fishing logbook
data from 2010 to 2019 to analyze skipjack resource
hotspots and coldspots in a space-time cube. The study
revealed 13 spatiotemporal patterns in skipjack Catch
per Unit Effort (CPUE). Hotspots (36.53\%) were
concentrated in the central area, predominantly showing
oscillating hotspots (21.25\%). The significant effect
of the eastern oscillating hotspot continues to be
enhanced and extends to the east. Coldspots constituted
63.47\% of the distribution, mainly represented by
intensifying coldspots (25.07\%). The
no-pattern-detected type (10.53\%) is distributed
between coldspots and hotspots. The fishing grounds
exhibited longitudinal oscillations of {3$^\circ
$}--6{$^\circ $} and latitudinal oscillations of
{1$^\circ $}--2{$^\circ $}. The spatial autocorrelation
of cold and hot spot distribution was strong, and the
spatiotemporal dynamic changes in skipjack resources
were closely related to the El Ni{\~n}o-Southern
Oscillation (ENSO) phenomenon. Notably, during
2011--2016, hotspots exhibited an eastward expansion
trend, which continued from 2017--2019 due to the
influence of fishery management measures, such as the
Vessel Day Scheme (VDS) system.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Natesan:2023:ARS,
author = "Balaji Natesan and Chuan-Ming Liu and Van-Dai Ta and
Raymond Liao",
title = "Advanced Robotic System with Keypoint Extraction and
{YOLOv5} Object Detection Algorithm for Precise
Livestock Monitoring",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "524",
day = "21",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100524",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/524",
abstract = "Molting is an essential operation in the life of every
lobster, and observing this process will help us to
assist lobsters in their recovery. However, traditional
observation consumes a significant amount of time and
labor. This study aims to develop an autonomous
AI-based robot monitoring system to detect molt. In
this study, we used an optimized Yolov5s algorithm and
DeepLabCut tool to analyze and detect all six molting
phases such as S1 (normal), S2 (stress), S3--S5 (molt),
and S6 (exoskeleton). We constructed the proposed
optimized Yolov5s algorithm to analyze the frequency of
posture change between S1 (normal) and S2 (stress).
During this stage, if the lobster stays stressed for
80\% of the past 6 h, the system will assign the
keypoint from the DeepLabCut tool to the lobster hip.
The process primarily concentrates on the S3--S5 stage
to identify the variation in the hatching spot. At the
end of this process, the system will re-import the
optimized Yolov5s to detect the presence of an
independent shell, S6, inside the tank. The optimized
Yolov5s embedded a Convolutional Block Attention Module
into the backbone network to improve the feature
extraction capability of the model, which has been
evaluated by evaluation metrics, comparison studies,
and IoU comparisons between Yolo's to understand the
network's performance. Additionally, we conducted
experiments to measure the accuracy of the DeepLabCut
Tool's detections.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Devergne:2023:ILT,
author = "Jimmy Devergne and V{\'e}ronique Loizeau and
Christophe Lebigre and Anne Bado-Nilles and Sophie
Collet and Olivier Mouchel and Ugo Iaria and
Marie-Madeleine {Le Gall} and Lauriane Madec and Cyril
Turi{\`e}s and Arianna Servili",
title = "Impacts of Long-Term Exposure to Ocean Acidification
and Warming on Three-Spined Stickleback
(\bioname{Gasterosteus aculeatus}) Growth and
Reproduction",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "523",
day = "21",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100523",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/523",
abstract = "The warming and acidification of surface waters as
predicted by the IPCC leads aquatic species to face
major multifaceted changes in their environment.
Although teleosts have efficient regulatory systems to
cope with these changes, such changes clearly have the
potential to impact their physiological functions.
Hence, it is crucial to estimate the ability of teleost
fishes to cope with multi-stresses to predict how they
will deal with future environments. In this context, we
investigated the joint effect of warming and
acidification on three-spined stickleback (Gasterosteus
aculeatus) from the juvenile stage to adulthood,
focusing on parameters linked to growth, sexual
maturation, and reproduction. Juvenile sticklebacks
were split in 2 climate scenarios: a ``Current''
scenario corresponding to the current seasonal
physico-chemical parameters of the water of the ``Rade
de Brest'' in France, and a ``RCP8.5'' scenario with a
warming of 3 {$^\circ $}C and an acidification of 0.4
pH units. After 7 months, fish in the RCP8.5 scenario
reached the same size and mass as those in the Current
scenario, but they needed greater amounts of food to
reach satiety. Furthermore, the mortality rate over the
experiment was higher in the RCP8.5 scenario. Muscle
lipid content, an indicator of energy reserves, was
lower in females in the RCP8.5 scenario, suggesting an
increased need for energy to maintain homeostasis and
other physiological functions or a divergence in energy
allocation strategy. Moreover, females exhibited lower
sexual maturation and egg quality under the RCP8.5
scenario, which could have contributed to the lower
fertilisation rate observed. Males were more resilient
to the RCP8.5 scenario, exhibiting only a trend for
lower kidney somatic index scores. Altogether, these
results suggest a delay and/or an inhibition of
gametogenesis and maturation in fish in warmed and
acidified waters. The analysis of blood sex steroid
concentrations, brain gene expression profiles, and
physiological indexes did not allow us to discriminate
between a delay and an inhibition of maturation in the
RCP8.5 scenario. Overall, these findings clearly
indicate that there is a long-term global impact of
combined acidification and warming on the mortality and
reproductive performance of three-spined stickleback.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Seret:2023:FRH,
author = "Bernard S{\'e}ret and Jean-Pascal Quod",
title = "First Records of a Hydrolagus Species ({Holocephali:
Chimaeridae}) from \geoname{Reunion Island} and
\geoname{Mayotte} (\geoname{Southwestern Indian
Ocean})",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "522",
day = "20",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100522",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/522",
abstract = "Two specimens of large blackish chimaeras of the genus
Hydrolagus were caught, one off Reunion Island and the
other off Mayotte in the southwestern Indian Ocean. The
specimens, an adult male of 710 mm BDL and a female of
870 m BDL, are described, compared to similar species
(i.e., having a large size, over 110 cm TL, dark
blackish colour, and rather long conical snout), and
tentatively identified to the small-eyed rabbitfish
Hydrolagus affinis, pending a taxonomic revision of the
large blackish chimaeras is completed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Filipsson:2023:WBJ,
author = "Karl Filipsson and Veronika {\AA}sman and Larry
Greenberg and Martin {\"O}sterling and Johan Watz and
Eva Bergman",
title = "Winter Behavior of Juvenile Brown Trout in a Changing
Climate: How Do Light and Ice Cover Affect Encounters
with Instream Predators?",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "521",
day = "20",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100521",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/521",
abstract = "During winter, stream fishes are vulnerable to
semi-aquatic predators like mammals and birds and
reduce encounters by being active in darkness or under
surface ice. Less is known about the behavior of fishes
towards instream piscivorous fishes. Here, we examined
how surface ice and light affected the anti-predator
behavior of juvenile brown trout (Salmo trutta
Linnaeus, 1758) in relation to piscivorous burbot (Lota
lota Linnaeus, 1758) and northern pike (Esox lucius
Linnaeus, 1758) at 4 {$^\circ $}C in experimental
flumes. Trout had lower foraging and swimming activity
and spent more time sheltering when predators were
present than when absent. In daylight, trout's swimming
activity was not affected by predators, whereas in
darkness trout were less active when predators were
present. Trout consumed more drifting prey during the
day when ice was present, and they positioned
themselves further upstream when under ice cover,
regardless of light conditions. Trout stayed closer to
conspecifics under ice, but only in the presence of
pike. Piscivorous fishes thus constitute an essential
part of the predatory landscape of juvenile trout in
winter, and thus loss of ice cover caused by climate
warming will likely affect trout's interactions with
predators.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vella:2023:PAS,
author = "Noel Vella and Adriana Vella",
title = "Phylogeographic Analyses of the Shortfin Mako,
\bioname{Isurus oxyrinchus} {Rafinesque}, 1810
({Chondrichthyes: Lamniformes}) from the
\geoname{Central Mediterranean Sea}, a Critically
Endangered Species in the Region",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "520",
day = "20",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100520",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/520",
abstract = "The Shortfin mako shark, Isurus oxyrinchus Rafinesque,
1810, is a globally distributed highly migratory
pelagic shark species, occurring mostly in temperate
and tropical regions, including the Mediterranean Sea
where it is by-caught during fishing activities
targeting other economically important fish species.
The aim of this study is to investigate the genetic
connectivity of the Shortfin mako from the central
Mediterranean Sea to previously studied populations.
The mtDNA control region (CR), 977 bp, of 37 I.
oxyrinchus specimens collected between 2004 and 2012
from landings in Malta were analysed, and we identified
nine haplotypes, including three newly discovered
haplotypes that may be unique to the Mediterranean Sea
and which represent 16.7\% of the studied individuals.
These haplotypes, together with variations in haplotype
frequencies, led to significant F ST and
{\SGMLvarphi}ST values between the Mediterranean
population and other global populations, with the
exception of that from the north Atlantic Ocean. This
study provides the first insight of the mtDNA CR
diversity of this critically endangered species in the
Mediterranean Sea and highlights the importance of
conserving this species in the region.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gough:2023:RVT,
author = "Brendan Gough and Alexandra Prouse and Michael A.
Dance and R. J. David Wells and Jay R. Rooker",
title = "Regional Variation in the Trophic Ecology of Wahoo
(\bioname{Acanthocybium solandri}) in the
\geoname{Western Atlantic Ocean}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "519",
day = "20",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100519",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/519",
abstract = "Intrinsic tracers, such as stable isotopes of carbon
and nitrogen, are common dietary markers that
accumulate in the muscle tissue of consumers and can be
used to determine the dietary sources and trophic
positions of consumers. The aim of this study was to
assess regional variation in the trophic ecology of
wahoo (Acanthocybium solandri) using bulk stable
isotopes. Muscle biopsies of wahoo were collected from
four regions in the western Atlantic Ocean: the eastern
Gulf of Mexico, western Gulf of Mexico, Northwest
Atlantic Ocean, and Caribbean Sea. Muscle tissue
{\delta} 13 C and {\delta} 15 N values for wahoo ranged
from {$-$15}.8{\SGMLpermil} to {$-$18}.8{\SGMLpermil}
and from 7.2{\SGMLpermil} to 12.8{\SGMLpermil},
respectively. Wahoo collected in the Caribbean Sea
displayed the highest mean {\delta} 13 C value
({$-$16}.3{\SGMLpermil}), and individuals from this
region were statistically different from the three
other regions sampled. Mean {\delta} 15 N values were
elevated for wahoo collected in the eastern and western
Gulf of Mexico (11.4{\SGMLpermil} and
11.1{\SGMLpermil}, respectively), and the values were
over 2{\SGMLpermil} higher than samples from the
Northwest Atlantic Ocean and Caribbean Sea. Trophic
position (TP) was estimated using {\delta} 15 N
baselines (zooplankton) and {\delta} 15 N wahoo values
for each region, and mean TP was 0.4 to 0.9 higher in
the Caribbean Sea relative to the three other regions,
suggesting that wahoo in this region feed on
higher-trophic-level prey. The results indicate that
{\delta} 15 N baselines and the trophic positions of
wahoo each vary as a function of their geographic
location, which supports the hypothesis that this
species feeds opportunistically throughout its range.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pompeu:2023:ISF,
author = "Paulo Santos Pompeu and L{\'\i}dia Wouters and Heron
Oliveira Hil{\'a}rio and Raquel Coelho Loures and
Alexandre Peressin and Ivo Gavi{\~a}o Prado and
F{\'a}bio Mineo Suzuki and Daniel Cardoso Carvalho",
title = "Inadequate Sampling Frequency and Imprecise Taxonomic
Identification Mask Results in Studies of Migratory
Freshwater Fish Ichthyoplankton",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "518",
day = "19",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100518",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/518",
abstract = "In South America, knowledge of major spawning sites is
crucial for maintaining migratory fish populations. In
this study, we aimed to understand the spatio-temporal
distribution of fish eggs in the upper S{\~a}o
Francisco River using high sampling frequency and DNA
metabarcoding identification. We evaluated the possible
effects of the non-molecular identification of eggs and
decreased sampling frequency on the determination of
spawning sites and major breeding periods. Collections
were carried out every three days from November 2019 to
February 2020. We found that, if we had assumed that
all of the free and non-adhesive sampled eggs belonged
to migratory species, as is usual in the literature,
this assumption would have been wrong for both the
spawning sites and the breeding periods. Moreover, any
decrease in the frequency of sampling could
dramatically affect the determination of the major
spawning rivers, and the spawning events of some of the
migratory species may not have been detected.
Therefore, without the proper identification and
adequate sampling frequency of eggs, important spawning
sites may be overlooked, leading to ineffective or
inappropriate conservation measures.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kiruba-Sankar:2023:ATK,
author = "Rajendran Kiruba-Sankar and Sirisha Adamala and
Jessica Barman and Kandasamy Saravanan and Jayasimhan
Praveenraj and Eswaran Yuvaraj and Gokhlesh Kumar and
Sheikh Zamir Ahmed",
title = "{Aboriginal} Tribe's Knowledge of the Endangered
Freshwater Turtle \bioname{Cuora amboinensis} in
\geoname{Car Nicobar}, a Remote Oceanic Island in the
\geoname{Bay of Bengal}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "517",
day = "18",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100517",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/517",
abstract = "Freshwater turtles are among the least studied faunas
in the Andaman and Nicobar Islands, India. Here, we
report the presence of freshwater turtles (Cuora
amboinensis) from Car Nicobar, a small remote island in
the Nicobar archipelago comprising the Andaman and
Nicobar Islands. Semi-structured interviews were
conducted with the people belonging to Nicobarese
tribes (n = 233) to gather their local ecological
knowledge as supportive information to obtain a better
understanding of the status of turtles in Car Nicobar.
Most interviewees (90\%) opined that freshwater turtles
are found to be rare or very rare on the Island. All
the respondents (100\%) expressed willingness to
contribute to future conservation projects.
Community-level awareness emerged as a prominent issue
for future conservation using the Garrett ranking
method. A stream network map was prepared using the
digital elevation model to visualize streams and
potential habitats of turtles. This report on the
freshwater turtles is essential from an ecological
perspective since information is scarce on the
freshwater turtles in Car Nicobar. This study
emphasizes the importance of the engagement of
stakeholders in conservation projects and recommends
providing adequate attention to the conservation and
protection of freshwater turtle diversity in Car
Nicobar.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cheng:2023:AAI,
author = "Xin Cheng and Fan Zhang and Xinjun Chen and Jintao
Wang",
title = "Application of Artificial Intelligence in the Study of
Fishing Vessel Behavior",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "516",
day = "18",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100516",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/516",
abstract = "Monitoring and understanding the behavior of fishing
vessels are important in facilitating effective
management, preventing illegal fishing, informing
fishing grounds and evaluating effects of harvests on
fishery resources. In recent decades, a large quantity
of real-time data of fishing vessels have become
available with the development of vessel-tracking
systems, making it possible to study the behavior of
fishing vessels in high spatial and temporal
resolutions. To effectively and efficiently deal with
the large amount of data, algorithms from artificial
intelligence (AI) are increasingly applied in the study
of fishing vessel behavior. In this paper, we first
introduce the various data sources for studying fishing
vessel behavior and compare their pros and cons.
Secondly, we review the AI methods that have been used
to monitor and extract the behavior of fishing vessels
from big data. Then, studies on the physical,
ecological and social mechanisms affecting the behavior
of fishing vessels were synthesized. Lastly, we review
the applications of fishing vessel behavior in fishery
science and management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luo:2023:EWS,
author = "Yuan-Yuan Luo and Xian-Can Chen and Rui-Lin Xie and
Zhuo-Hao Ruan and Zhi-Qiang Lu and Liang-Sen Jiang and
Yi-Fu Li and Wen-Sheng Liu",
title = "The Effect of Water Spinach on the Water Quality,
Antioxidant System, Non-Specific Immune Response,
Growth Performance, and Carbon Balance in Red Tilapia
Production",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "515",
day = "18",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100515",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/515",
abstract = "In this study, the compound aquaculture model of red
tilapia (O. mossambicus albina $ \times $ O. urolepis
hornorum) and water spinach (Ipomoea aquatica) was used
to investigate the effect of water spinach rafts on the
water quality, antioxidant system, non-specific immune
response, and growth performance of red tilapia and the
carbon balance of payments. Red tilapia is
characterized by its high adaptability to different
production environments and food sources, as one of the
most productive fish in aquaculture, and is well
accepted in the market due to its nutritional and
organoleptic characteristics. The experiment lasted for
nine weeks and included two systems: the red
tilapia-water spinach raft aquaponics (AP) system with
10\% cover ratio with water spinach floating beds, and
the aquatic monoculture (AM) system with only red
tilapia. The total phosphorus (TP), total nitrogen
(TN), and nitrate nitrogen (NO {$-$} 3 -N) in the AM
were higher than those in AP from the fifth to ninth
week. On the second, third, fifth, and sixth weeks, the
ammonia nitrogen (NH 4 + -N), in the AM was higher than
those in the AP. From the seventh week, the pH of the
AM was significantly lower than the AP, while the
nitrite nitrogen (NO {$-$} 2 -N) was significantly
higher than the AP. The water quality index of the AP
was better than that of the AM, indicating that water
spinach can remove the nutrients from aquaculture water
bodies. The average daily gain and specific growth rate
(SGR) of fish in AP were higher than those in the AM.
The acid phosphatase (ACP), alkaline phosphatase (AKP),
and catalase (CAT) activities in the hepatopancreas of
red tilapia in the AP were also significantly higher
than those in the AM, while the malondialdehyde (MDA)
in the AP was lower than the AM. The serum ACP and CAT
of red tilapia in the AP were also higher than those in
the AM, while the MDA of fish in the AP was lower than
the AM. The results showed that both the experimental
group and the control group were carbon sources and
released greenhouse gases into the atmosphere, but the
total carbon emissions of the red tilapia and the water
spinach symbiotic system in the experimental group was
significantly lower than that of the control group (p
{$<$} 0.05). These results demonstrated that the
application of water spinach rafts in aquaponics can
not only improve the water quality, but also improve
the growth performance, antioxidant system and
non-specific immune responses of red tilapia, while
promoting the utilization of organic matter in the
aquaculture system, improving the ecological benefits
in terms of the carbon income and expenditure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hamzaoui:2023:IDL,
author = "Mahdi Hamzaoui and Mohamed Ould-Elhassen Aoueileyine
and Lamia Romdhani and Ridha Bouallegue",
title = "An Improved Deep Learning Model for Underwater Species
Recognition in Aquaculture",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "514",
day = "16",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100514",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/514",
abstract = "The ability to differentiate between various fish
species plays an essential role in aquaculture. It
helps to protect their populations and monitor their
health situations and their nutrient systems. However,
old machine learning methods are unable to detect
objects in images with complex backgrounds and
especially in low-light conditions. This paper aims to
improve the performance of a YOLO v5 model for fish
recognition and classification. In the context of
transfer learning, our improved model FishDETECT uses
the pre-trained FishMask model. Then it is tested in
various complex scenes. The experimental results show
that FishDETECT is more effective than a simple YOLO v5
model. Using the evaluation metrics Precision, Recall,
and mAP50, our new model achieved accuracy rates of
0.962, 0.978, and 0.995, respectively.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Patil:2023:CMP,
author = "Maheshkumar Prakash Patil and Jong-Oh Kim and Seung
Hyun Yoo and Yong Bae Seo and Yu-Jin Lee and Jin-Koo
Kim and Shin-Ichi Kitamura and Gun-Do Kim",
title = "Complete Mitogenome and Phylogenetic Analysis of a
Marine Ray-Finned Fish, \bioname{Alcichthys elongatus}
({Perciformes: Cottidae})",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "513",
day = "16",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100513",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/513",
abstract = "Alcichthys elongatus is the only species in the genus,
and this work is the first to provide a comprehensive
mitogenome analysis of this species. The A. elongatus
mitogenome was 16,712 bp long, with biased A + T
content (52.33\%), and featured thirteen protein-coding
genes (PCGs), twenty-two tRNAs, two rRNAs, and the
control region (D-loop). The H strand encoded
twenty-eight genes (twelve PCGs, fourteen tRNA, and two
rRNA) and the control region, whereas the L strand
encoded the remaining nine genes (ND6 and eight tRNA).
Except for COXI, which started with GTG, all PCG
sequences started with ATG and ended with TAA (ND4L,
ND5, COXI, ATP8) or TAG (ND1, ND6) termination codons,
with some (ND2, ND3, ND4, COXII, COXIII, ATP6, Cytb)
having an incomplete termination codon. Except for
tRNA-serine-1 (trnS), the majority of the tRNAs
exhibited characteristic cloverleaf secondary
structures. Based on 13 PCGs, phylogenetic analysis
placed A. elongatus in the same clade as Icelus
spatula. This genomic data will be useful for species
identification, phylogenetic analysis, and population
genetics.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rind:2023:EDC,
author = "Khalid Hussain Rind and Syed Sikandar Habib and Javed
Ahmed Ujan and Francesco Fazio and Saira Naz and Aima
Iram Batool and Mujeeb Ullah and Sobia Attaullah and
Khayyam Khayyam and Khalid Khan",
title = "The Effects of Different Carbon Sources on Water
Quality, Growth Performance, Hematology, Immune, and
Antioxidant Status in Cultured \geoname{Nile} Tilapia
with Biofloc Technology",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "512",
day = "14",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100512",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/512",
abstract = "The biofloc technology system (BFT) is considered to
be one of the sustainable aquaculture systems, which is
based on the principle of nutrient recycling with the
addition of a carbon source to give dominance to
heterotrophic microorganisms. The objective of this
study was to evaluate the effect of sugar cane molasses
and tapioca flour as carbon sources on the water
quality, growth, hematology, immune status, and
non-specific antioxidant status of Oreochromis
juveniles. Methodologically, the experiment was carried
out for 10 weeks on 225 juvenile Nile tilapia with
initial body weights of 47.0 {\pm} 1.3 g that were
randomly distributed in 09 tanks (1000 L) with a
stocking density of 25 tilapias per tank; the
treatments were: BFT + SM (S molasses), BFT + TF
tapioca flour (TF), and a control with no carbon source
added. The control group was fed 100\% feed, while the
BFT experimental groups were fed microbial flocs along
with 75\% feed. The results revealed that the water
quality parameters were affected by the carbon sources,
but were adequate for normal fish welfare, and the
biofloc volume was higher (28.94) with the TF carbon
source. The growth performance, such as weight gain
(98.61), survival (99.01), and improved feed conversion
ratio (FCR) (1.69), was recorded in BFT + TF.
Significant improvements in WBCs, HCT, HB, lymphocytes,
plasma proteins, albumin, and non-specific immune
factors (lysozyme activity, immunoglobulins levels, and
ACH50) were observed in biofloc-reared fish with
tapioca flour as the carbon source compared to the
control and sugarcane molasses groups. Moreover,
significant increases in catalase (CAT) and superoxide
dismutase (SOD) were found in the biofloc-reared fish
with different carbon sources. In conclusion, the use
of BFT + TF was found to affect improving the water
quality, growth, hematology, immunity, and antioxidant
status of juvenile Tilapia.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luo:2023:ENI,
author = "Zhengli Luo and Yanbo Zhou and Ning Liu and Liangming
Wang and Yan Liu and Binbin Shan and Manting Liu and
Cheng Chen and Changping Yang and Dianrong Sun",
title = "Ecological Niche and Interspecific Association of the
Main Fishes in the Coastal Waters of \geoname{Hainan
Island, China}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "511",
day = "13",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100511",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/511",
abstract = "This study explored the ecological niche and
interspecific relationships among the main fish species
in the coastal waters of Hainan Island based on data
from fishery stock surveys conducted in the spring and
autumn of 2022. The methods of chi-square test,
percentage of co-occurrence (PC), association
coefficient (AC), cluster analysis, and redundancy
analysis were used to analyze the interspecific
associations and influence of environmental factors on
the ecological niches of fish communities. According to
the cluster analysis of niche breadth, 20 main fishes
could be divided into broad, medium, and narrow niche
species. There were four and eight broad niche species
in spring and autumn, respectively. The ranges of niche
overlap values were 0.001--0.91 in spring and 0--0.87
in autumn, indicating that the species differed and
were similar in their ability to utilize resources,
survive in habitats, and prey. According to the
variance ratio and statistic value W, the main fishes
in spring showed a significant positive association,
whereas those in autumn showed a positive association,
but not at a significant level, indicating that the
main fishes in spring were more closely associated with
each other. There were 56 species pairs in the 2
seasons that were significantly associated ({\chi} 2
{\geq} 3.841). AC and PC tests revealed that the
interspecific association was strong and tended to be
positive. According to the redundancy analysis,
environmental factors such as surface temperature,
water depth, and pH significantly affected the main
fishes in spring, while environmental factors such as
dissolved oxygen, bottom temperature, surface salinity,
and pH significantly affected those in autumn.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sadler:2023:RHF,
author = "Daniel E. Sadler and Phillip C. Watts and Silva
Uusi-Heikkil{\"a}",
title = "The Riddle of How Fisheries Influence Genetic
Diversity",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "510",
day = "13",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100510",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/510",
abstract = "Overfishing drives population decline, which in turn
drives loss of genetic diversity. Many studies provide
evidence of declines in genetic diversity; however,
controversy exists within the literature, as some
studies show evidence of no change in genetic diversity
despite decades of overharvesting. The apparent
discrepancy in the literature should therefore be
examined to understand what biological and ecological
processes are driving the differences in results. Here,
we assess how different factors contribute to
fisheries-induced susceptibility to declines in genetic
diversity by first focusing on the different roles of
genetic markers. Second, we assess how habitat type and
conditions contribute to loss of genetic diversity.
Third, we assess how life history and physiology
affects catchability and loss of genetic diversity.
Finally, we discuss how coinciding abiotic and biotic
factors influence the intensity of genetic loss. We
find a multitude of these factors could be interacting
to influence how results are perceived and how intense
the loss of genetic diversity can be. Future studies
should carefully consider the methodology of genetic
analysis used, as well as considerations of life
history and ecology of the target species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Santos:2023:GPH,
author = "Fabio A. C. Santos and Felipe S. Batista and Andr{\'e}
S. Souza and Gustavo S. C. Julio and Gisele C. Favero
and Jos{\'e} F. V. Junior and S{\'\i}lvio T. Costa and
Carla C. Zeppenfeld and Nadia H. Bianchini and Berta M.
Heinzmann and Bernardo Baldisserotto and Ronald K.
Luz",
title = "Growth Performance and Histomorphology of Intestine,
Skin, Gills and Liver of Juvenile \bioname{Colossoma
macropomum} Fed Diets Containing Different Levels of
the Essential Oil of \bioname{Nectandra grandiflora}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "509",
day = "11",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100509",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/509",
abstract = "The present study evaluated different levels of the
essential oil of Nectandra grandiflora (EONG) in the
diet of juvenile Colossoma macropomum. The juveniles
(0.75 {\pm} 0.05 g) were fed four experimental diets
with differing levels of EONG (0, 0.5, 0.75, and 1.50
mL/kg). After 20 days, the weight, daily weight gain,
specific growth rate, and daily feed consumption per
fish were highest for the fish fed 0.5 mL EONG/kg,
while after 30 days, only the weight and daily feed
consumption per fish for that diet remained highest.
The viscerosomatic index was highest for the fish fed
0.5 mL EONG/kg while the hepatosomatic index was higher
for the groups that received dietary EONG for 30 days.
The juveniles fed 1.5 mL EONG/kg showed a proliferative
response of the mucous cells in the gills, but the
proliferation of these lysozyme- and
immunoglobulin-secreting mucosal cells was higher for
the skin of the fish of all EONG levels, compared to
that of the control fish. The fish fed 0.5 mL EONG/kg
had the greatest height and width of intestinal villi.
The two highest levels of dietary EONG supplementation
(0.75 and 1.50 mL/kg) reduced the hepatocyte dimensions
but did not modify the centrolobular vein area. Dietary
supplementation with 0.5 mL EONG/kg improved the
growth, gut health, and immune response of juvenile C.
macropomum.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shelledy:2023:RST,
author = "Katharine N. Shelledy and Amy Y. Olsen and Alexander
Tanz and Megan H. Williams and Jeff Christiansen and
Heidi Wilken and Chris {Van Damme} and Shawn Larson",
title = "Rockfish Species Trends in \geoname{Puget Sound,
Washington, USA}, 2009--2023",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "508",
day = "11",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100508",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/508",
abstract = "Rockfishes (Sebastes spp.) are a long-lived,
understudied, multi-species fish assemblage in inland
Washington (USA) waters. Driven by large-scale fishery
removals in the 1970s, their numbers declined and have
yet to recover, perhaps due to slow maturation and rare
recruitment success. Since then, rockfish fishing
restrictions have increased within Puget Sound,
culminating in a 2010 total ban. Here, we analyze
rockfish community trends (abundance and Shannon
Diversity) within Puget Sound from 2009 to 2023. Our
dataset includes 157 recorded dive transects from nine
rocky or artificial reef habitats at depths 5--24 m
throughout four Puget Sound basins: Admiralty Inlet (2
sites), Central Puget Sound (4 sites), Hood Canal (2
sites), and South Puget Sound (1 site). Significant
differences in community species composition between
basins were observed; we noted more Black (S.
melanops), Yellowtail (S. flavidus), and Puget Sound
(S. emphaeus) rockfish and fewer Brown rockfish (S.
auriculatus) at sites in Admiralty Inlet vs. Central
Puget Sound. Adult rockfish counts and Shannon
Diversity varied seasonally, with higher rockfish
counts and diversity indices in summer/autumn vs.
winter/spring. Notably, we observed that total adult
rockfish count per survey tended downward over time,
driven partly by significant downward trends in Hood
Canal Copper rockfish (S. caurinus) counts. We
recommend continued monitoring and additional
investigation into what factors besides fishing may be
driving the trends reported here.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhao:2023:HLG,
author = "Lingmin Zhao and Lixing Huang and Yingxue Qin and Dou
Yang and Jiaonan Zhang and Jiaolin Zhang and Qingpi
Yan",
title = "How the \gene{luxR} Gene Affects the Pathogenicity of
\bioname{Pseudomonas plecoglossicida} and the Immune
Response of \bioname{Epinephelus coioides}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "507",
day = "11",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100507",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/507",
abstract = "This study aimed to investigate the effect of reduced
expression of the luxR gene on the virulence of
Pseudomonas plecoglossicida and the immune response of
Epinephelus coioides. To achieve this, RNA interference
(RNAi) was used to silence the luxR gene, and the
pathogenicity of wild-type and luxR -RNAi strains of P.
plecoglossicida, as well as the immune response of
Epinephelus coioides to the infection of these two
strains, were compared. The mutant strain with the
highest silencing efficiency of 70.1\% was selected for
subsequent analysis. Silencing the luxR gene in the
mutant strain resulted in a significant 30\% reduction
in mortality rates in artificially infected Epinephelus
coioides compared to the wild-type strain.
Transcriptome analysis revealed that the host
transcriptome, particularly in the spleens of infected
Epinephelus coioides, was markedly altered by the
silencing of the luxR gene in the mutant strain.
Tilapia infected with the luxR -RNAi strain exhibited
altered immune defenses, with changes in gene
expression primarily in the NOD-like receptor (NLR)
signaling pathway. These results suggest that the luxR
gene plays a crucial role in the host's resistance to
pathogen invasion, and reducing its expression could
decrease quorum sensing (QS) signals while increasing
the expression of the IL-1{\beta} gene in the host's
NLR pathway. This effect may lead to a pro-inflammatory
response that enhances the immune response to
infection. Further investigation of these mechanisms
may lead to innovative approaches to treating bacterial
infections.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:PPD,
author = "Jiyuan Li and Yanfeng Wang and Teng Guo and Shihong Xu
and Guang Gao and Feng Liu and Xiaoyang Guo and Yanduo
Wu and Haixia Zhao and Jun Li",
title = "Phenethylamine Is a Potential Density Stress Pheromone
in Turbot (\bioname{Scophthalmus maximus})",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "506",
day = "11",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100506",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/506",
abstract = "Pheromones play a vital role in regulating fish
behavior, including reproduction, aggregation, hazard
recognition and food location. To gain a better
understanding of chemical communication in fish
produced by density changes, this study analyzed the
metabolites released by turbot (Scophthalmus maximus)
under different stocking densities. The experiment was
conducted at low (LD: 3.01 kg/m 2), medium (MD: 6.62
kg/m 2) and high (HD: 10.84 kg/m 2) densities for 15
days. High-throughput non-targeted metabolomics
(LC-MS/MS) was used to identify variations in
metabolites released into the aquatic environment by
turbot at different densities. Results showed that 29
and 47 metabolites were significantly upregulated in
the MD and HD groups, respectively, compared with the
LD group. Among them, hexadecanedioic acid, xanthine,
phenethylamine, proline and styrene were significantly
upregulated in the MD vs. LD, HD vs. MD and HD vs. LD.
The VIP diagram of OPLS-DA alignment showed that
phenethylamine was the most important metabolite shared
by MD vs. LD, HD vs. MD and HD vs. LD. Key gene changes
in the GH/IGF-1 signaling pathway, HPI axis of turbot
were studied using qRT-PCR for density treatment. The
results demonstrated that the expression of GH, GHR and
IGF-1 was significantly lower, while the expression of
CRH and ACTH was higher in the HD group. Additionally,
plasma levels of cortisol, glucose, triglycerides and T
3 were also highest in the HD group compared with the
LD and MD groups. Phenylethylamine concentration was
positively correlated with the HPI axis and negatively
correlated with the GH/IGF-1 signaling pathway. To
investigate the impact of phenethylamine accumulation
on turbot, an acute treatment experiment with
phenethylamine was set up. Its concentration in the
aquatic environment was set at 0 (CON), 10 {$-$7} (LP)
and 10 {$-$5} (HP) mol/L via exogenous addition, and
turbot were exposed to these environments for 2 days.
There was a high degree of concordance between the
GH/IGF-1 signaling pathway (GH, GHR, IGF-1), HPI axis
(CRH, ACTH) and plasma physiological changes (cortisol,
glucose, triglycerides, T 3) in the
phenethylamine-treated group and the density-treated
group. Therefore, accumulation of phenethylamine with
increasing stocking density may be a potential cause of
density stress. Phenylethylamine has a dose-dependent
and trace effect as a pheromone.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hamzaoui:2023:OXP,
author = "Mahdi Hamzaoui and Mohamed Ould-Elhassen Aoueileyine
and Lamia Romdhani and Ridha Bouallegue",
title = "Optimizing {XGBoost} Performance for Fish Weight
Prediction through Parameter Pre-Selection",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "505",
day = "10",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100505",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/505",
abstract = "Fish play a major role in the human nutritional
system, and farmers need to know the accurate
prediction of fish weight in order to optimize the
production process and reduce costs. However, existing
prediction methods are not efficient. The formulas for
calculating fish weight are generally designed for a
single species of fish or for species of a similar
shape. In this paper, a new hybrid method called
SFI-XGBoost is proposed. It combines the VIF (variance
inflation factor), PCC (Pearson's correlation
coefficient), and XGBoost methods, and it covers
different fish species. By applying GridSearchCV
validation, normalization, augmentation, and encoding
techniques, the obtained results show that SFI-XGBoost
is more efficient than simple XGBoost. The model
generated by our approach is more generalized,
achieving accurate results with a wide variety of
species. Using the r2_score evaluation metric,
SFI-XGBoost achieves an accuracy rate of 99.94\%.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2023:DSG,
author = "Yang Zhou and Li Xu and Zhongtang He and Weijie Cui
and Qun Lu and Jianguang Qin and Shengqi Su and Tao
He",
title = "Discrimination of \bioname{Schizothorax grahami}
({Regan}, 1904) Stocks Based on Otolith Morphology",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "504",
day = "10",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100504",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/504",
abstract = "Otoliths grow throughout a fish's life and are
important for identifying fish stocks and fish age.
This study aims to differentiate different stocks of
Schizothorax grahami (Regan, 1904) in the Chishui
River, an upper reach of the Yangtze River, by otolith
morphology. The otolith morphology of S. grahami from
three different river sections was analyzed using the
Shape Index, Fourier coefficients, and wavelet
coefficients. The composite discrimination success rate
of the Shape Index was 59.7\%, and it was difficult to
distinguish in the scatter plots. In contrast,
canonical principal coordinate scatter plots clearly
showed three distinguished stocks. The above results
indicate that otolith morphology can discriminate
between stocks in plateau endemic fish, and several S.
grahami stocks may be separately managed in the Chishui
River.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sun:2023:TDF,
author = "Lufeng Sun and Xiuqi Li and Guancang Dong and Xuri
Cong",
title = "Taxonomic Diversity of Fish Species in the Lower
Reaches of the \geoname{Yellow River}",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "503",
day = "09",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100503",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/503",
abstract = "In order to study the community of fishery resources,
five sampling sections were set up in the lower Yellow
River from 2019 to 2022 to investigate the diversity of
fishery resources. A total of 54 fish species were
identified in the survey, belonging to 9 orders, 19
families, and 48 genera. The number of species reached
its highest level since the Integrated Water Regulation
of the Lower Yellow River in 1999. The original rare
and endangered fish species in the lower reaches of the
Yellow River, such as Coilia nasus, Rhinogobio nasutus,
and Pseudobagrus ussuriensis, have reappeared in the
lower reaches. The population and abundance of main
economic fish and common small-sized fish in the lower
reaches of the Yellow River have increased. These signs
all indicate that, and the fishery resources are
recovering slowly in the lower Yellow River. By
comparing the fish resources of various investigation
sections, it was found that there are differences in
fish diversity: the closer to the estuarine waters, the
higher the variety of fish in the Yellow River at the
upper level of the taxonomic category. However, that
section of the lower Yellow River where species
evenness, species diversity, and community structure of
fishery resources were most stable was the confluence
of Dongping Lake into the Yellow River, which has the
most significant number of fish species, the smallest
{\Lambda} + value, and the most extensive G-F index.
The above results indicate that Dongping Lake, as the
only lake connected to the lower reaches of the Yellow
River, plays an essential role in replenishing fish
resources in the lower reaches of the Yellow River.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Song:2023:BRS,
author = "Yifan Song and Shengmao Zhang and Fenghua Tang and
Yongchuang Shi and Yumei Wu and Jianwen He and Yunyun
Chen and Lin Li",
title = "Behavior Recognition of Squid Jigger Based on Deep
Learning",
journal = j-FISHES,
volume = "8",
number = "10",
pages = "502",
day = "08",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8100502",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:14 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/10/502",
abstract = "In recent years, with the development of pelagic
fishing, the working environment and monitoring of crew
(squid jigger) members have become increasingly
important. However, traditional methods of pelagic
human observers suffer from high costs, low coverage,
poor timeliness, and susceptibility to subjective
factors. In contrast, the Electronic Monitoring System
(EMS) has advantages such as continuous operation under
various weather conditions; more objective,
transparent, and efficient data; and less interference
with fishing operations. This paper shows how the 3DCNN
model, LSTM+ResNet model, and TimeSformer model are
applied to video-classification tasks, and for the
first time, they are applied to an EMS. In addition,
this paper tests and compares the application effects
of the three models on video classification, and
discusses the advantages and challenges of using them
for video recognition. Through experiments, we obtained
the accuracy and relevant indicators of video
recognition using different models. The research
results show that when NUM_FRAMES is set to 8, the
LSTM+ResNet-50 model has the best performance, with an
accuracy of 88.47\%, an F1 score of 0.8881, and an map
score of 0.8133. Analyzing the EMS for pelagic fishing
can improve China's performance level and management
efficiency in pelagic fishing, and promote the
development of the fishery knowledge service system and
smart fishery engineering.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:EGB,
author = "Gangfu Chen and Jing Xu and Min Wu and Huatao Li and
Qihui Yang and Lin Feng",
title = "Extract of \bioname{Ginkgo biloba} Leaves ({EGb})
Decrease Lipid Oxidation in Fish Feed and Meat and
Enhance Growth and Antioxidant Capacity in {Jian} Carp
(\bioname{Cyprinus carpio} var. {Jian})",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "564",
day = "20",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110564",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/564",
abstract = "Firstly, a linoleic and linolenic acid emulsion were
incubated with petroleum ether extract, ethyl acetate
extract (EAE), ethanol extract and aqueous extract of
Ginkgo biloba leaves. The flavonoids content, total
antioxidant capacity (T-AOC) and metal-chelating
ability (MCA) were determined in EGbs above. Results
showed that the extracts of Ginkgo biloba leaves (EGbs)
inhibited the lipid oxidation in material above. Of all
of EGbs, EAE showed the strongest T-AOC, MCA and
protective effects against the lipid oxidation. Next,
fish feeds were incubated with graded levels of EAE.
The results showed that EAE inhibited lipid oxidation
in fish feeds. The optimal inclusion levels of EAE for
minimizing lipid oxidation were 4.26 g kg {$-$1} feeds.
The effect of EGbs on the lipid oxidation may be
closely associated with their flavonoid content.
Finally, juvenile carp (14.8 {\pm} 0.4 g) were fed with
EAE at concentrations ranging from 0.0 to 6.0 g kg
{$-$1} for 60 days. Current data displayed that dietary
EAE increased the growth performance of fish. This
result of EAE may be ascribed to its enhancing effect
on the activity of digestive and absorptive enzymes and
antioxidant capacity in digestive organs of fish.
Furthermore, dietary EAE decreased the
hot-drying-induced lipid oxidation in fish meat through
inhibiting the induction effect of hemoglobin in
erythrocytes. Our study suggests that EGb can be
considered as a potential natural antioxidant for fish
and fish feed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mpomwenda:2023:COC,
author = "Veronica Mpomwenda and Tumi T{\'o}masson and J{\'o}n
Geir P{\'e}tursson and Da{\eth}i Mar
Krist{\'o}fersson",
title = "From Co-Operation to Coercion in Fisheries Management:
The Effects of Military Intervention on the
\geoname{Nile} Perch Fishery on \geoname{Lake Victoria}
in \geoname{Uganda}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "563",
day = "20",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110563",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/563",
abstract = "In 2017, Uganda's small-scale inland fisheries
underwent a significant transformation, shifting from
local co-management to state military enforcement owing
to ineffective enforcement of regulations and declining
exports. Employing a mixed-methods approach and
blending qualitative and quantitative data, we assessed
the impact of military intervention on Lake Victoria's
Nile perch fishery, focusing on fishing effort, catch,
and exports. Our findings indicate that military
operations adhered to regulations, gaining primary
support from key stakeholders, specifically motorized
fishing operators. Consequently, between 2016 and 2020,
legal fishing activities experienced substantial
growth. By 2021, approximately 90\% of Nile perch
catches were made by motorized vessels using longlines
and gillnets, despite a declining trend in
catch-per-unit effort. Between 2015 and 2021, the Nile
perch fishery saw changes: boat seines made up about
5\% of motorized fleet catches in 2021, while catches
in paddled vessels increased from 20\% to over 50\%,
suggesting a potential role in the growing longline
fishery. Therefore, the current management approach
does not increase catches or exports compared with the
co-management period. The observed decline in
catch-per-unit effort among motorized gillnets suggests
overcapacity. Further research is needed to comprehend
the broader sociological and ecological impacts of the
present enforcement strategy for sustainable fishery
management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Queller:2023:LLE,
author = "Philip S. Queller and Elena R. M. Adams and Molly E.
Cummings",
title = "Life-Long Experience with Male Mating Tactics Shapes
Spatial Cognition and Coercion Evasion in Female
Swordtails",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "562",
day = "20",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110562",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/562",
abstract = "Social experiences can shape adult behavior and
cognition. Here, we use El Abra swordtails (Xiphophorus
nigrensis) to assess how life-long experience with
different male mating tactics shapes coercion evasion
ability and female spatial cognition. We raised females
from birth to adulthood in environments that varied by
male mating tactic: coercers only, courtship displayers
only, coercers and displayers together,
mixed-strategists, and female only. In adulthood, we
tested females' behavioral responses to a coercive male
and spatial cognition in a maze. Females reared with
only displayers were significantly worse at distancing
themselves from the coercive male than females raised
with coercers and displayers and females raised with
only coercers. Females raised with a single mating
tactic (either courtship display or coercion) exhibited
significantly higher accuracy in the spatial maze than
females from other rearing groups, and showed
significant reduction in total errors (courtship
display group) or latency to reward (coercion group)
over successive trials. These more predictable
environments (one tactic), and not the more complex
environments (two tactics), showed evidence for spatial
learning. The results are discussed in light of the
existing literature on two components of environmental
change (environmental predictability and the certainty
with which cues predict the best behavioral response)
and their effect on the development of cognitive
abilities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Antognazza:2023:CAA,
author = "Caterina M. Antognazza and Anja Palanda{\'c}i{\'c} and
Giovanni B. Delmastro and Giuseppe Crosa and Serena
Zaccara",
title = "Correction: {Antognazza et al. Current and Historical
Genetic Variability of Native Brown Trout Populations
in a Southern Alpine Ecosystem: Implications for Future
Management. {{\booktitle{Fishes}}} 2023, {\bf 8},
411}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "561",
day = "20",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110561",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Antognazza:2023:CHG}.",
URL = "https://www.mdpi.com/2410-3888/8/11/561",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yu:2023:IMS,
author = "Hyun-Ji Yu and Young Il Seo and Jae-Hyeong Yang and
Jeong-Ik Baek and Seong hun Kim",
title = "Investigating the Mesh Size Selectivity of Olive
Flounder (\bioname{Paralichthys olivaceus}) Gillnets
for Fisheries Resource Management in the \geoname{East
Sea}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "560",
day = "20",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110560",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/560",
abstract = "This study aimed to evaluate the mesh selectivity of
gillnets used in olive flounder (Paralichthys
olivaceus) fishing by examining the distribution of
total length (TL) across various mesh sizes. A marine
fishing experiment was conducted at Gajin Harbor in
Goseong County, Gangwon Province, employing
experimental gear with four different mesh sizes: 90,
105, 135, and 150 mm. Mesh selectivity analysis
involved a comparative assessment of selectivity curve
methods through normal, log-normal, and bi-normal
functions. Among these, the bi-normal function
demonstrated the best fit for estimating the mesh
selectivity curve within the SELECT model. The critical
finding was the net size at which 50\% of the minimum
landing size (35 cm in TL) of olive flounder was
selected, ranging from 108.7 to 121.1 mm. According to
the bi-normal function curve, this size was estimated
as 114.0 mm. Our findings indicate that using a mesh
size larger than the theoretically estimated size in
mesh selectivity analysis can further decrease the
catch of immature individuals. These findings provide
essential data for devising strategies to efficiently
utilize and manage olive flounder resources.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:SDM,
author = "Menghui Li and Xuehui Wang and Feiyan Du and Shuai
Peng and Lianggen Wang and Dianrong Sun and Yuezhong
Wang and Pimao Chen and Yongsong Qiu",
title = "Spatial Distribution of Major Fish Species Catches and
Their Relationship with Environmental Factors in the
\geoname{Beibu Gulf, South China Sea}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "559",
day = "20",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110559",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/559",
abstract = "This study focused on major fish species caught from
different ecological habitats to analyze the habitat
changes in economically important fish in the Beibu
Gulf. The maximum entropy model was constructed based
on the data from 26 voyages obtained through the otter
trawl fishery stock surveys conducted in the Beibu Gulf
from 2006 to 2018. A total of 10 taxa of major fish
catches, belonging to 4 families, 1 genus, and 5
species, were analyzed for their distribution
characteristics in potential habitats during various
ecological periods, specifically the withered and
high-water periods, as well as their relationships with
crucial environmental factors. During both the withered
and high-water periods, the average area under the
curve was 0.927, and all models had values exceeding
0.9. Salinity was identified as the principal factor
during both periods. The salinity niche of Acropoma
japonicum and the primary productivity niche of
Sciaenidae were identified as the most restricted
factors. The total distribution area of potentially
suitable regions for major fish catches extended
between {106$^\circ $}30{\prime}--109{$^\circ
$}45{\prime} E and {17$^\circ $}30{\prime}--20{$^\circ
$}45{\prime} N, and the area was lesser during the
withered period. During the withered period, the
distribution areas of Sciaenidae, Leiognathidae, and
Saurida increased, while the distribution area of
Trachurus japonicus increased during the high-water
period. Sciaenidae and Trachurus japonicus exhibited
the highest rates of change at 6.22\% and 10.92\%,
respectively. This indicates that the high-water period
may expand the range of suitable habitats for large
catches that have an ideal habitat status in the Beibu
Gulf. Therefore, it is crucial to anticipate the
potential fish habitats in the Beibu Gulf and clarify
their spatial distribution patterns.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2023:TCL,
author = "Fan Zhou and Xuelin Zhang and Gaohua Yao and Xiaoming
Chen and Ming Qi and Qin Zhou and Ningyu Zhu and
Qinghui Meng and Yu Zhang and Xueyan Ding",
title = "Transcriptomic Comparison of Liver Tissue across
Different Largemouth Bass (\bioname{Micropterus
salmoides}) Strains",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "558",
day = "19",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110558",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/558",
abstract = "Over the past few years, China has become a hotspot
for the domestication of the commercially valuable
largemouth bass (Micropterus salmoides). Although the
food preference of this fish has been studied, little
is known about the genes regulating its growth.
Population breeding was performed using two indigenous
strains (QT1 and QT2), with the results showing that
the organ/body ratio, abdominal fat rate and the body
weight gain of QT1 and QT2 were higher than for the
offspring YL1 and Y3 which are extensively cultured in
China. Subsequent RNA sequencing (RNA-Seq) allowed for
the identification of potential genes and pathways
involved in growth performance. Overall, the
transcriptome analysis generated 89,056 transcripts and
42,529 Unigenes. A PCA revealed significant differences
between QT1 and the other three strains, while the
other three strains did not show much difference. A
KEGG enrichment analysis of differentially expressed
genes showed that steroid biosynthesis was the most
enriched pathway among the four strains. These pathways
could be related to the growth of largemouth bass. In
addition, a co-expression network analysis suggested a
strong interaction between liver steroid biosynthesis
and the genes for photosynthesis, secondary metabolism
and stress response. Taken together, the above results
can provide new insights into the liver metabolism of
different strains of largemouth bass during culture and
provide references for the subsequent domestication and
breeding programs of largemouth bass.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mundahl:2023:CRF,
author = "Neal D. Mundahl and Kelsey A. Hoffmann",
title = "Condition, Reproductive Fitness, and Fluctuating
Asymmetry in Brook Stickleback: Responses to
Anthropogenic Runoff",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "557",
day = "19",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110557",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/557",
abstract = "Multiple indicators have been used to assess the
degree of exposure of fish to anthropogenic chemicals
in their stream habitats. We hypothesized that brook
stickleback (Culaea inconstans) in a headwater stream
receiving urban and agricultural runoff (South Fork
Whitewater River, SFWR) would exhibit poorer condition,
reduced reproductive fitness, and a greater left side
to right side morphological asymmetry (i.e.,
fluctuating asymmetry or FA) than fish from a nearby
headwater stream with a forested drainage basin (Garvin
Brook). Male and female fish were collected from both
streams just prior to spawning in 2013--2015. In 2013
and 2014, fish were assessed for overall condition
(Fulton's K), internal measures of condition
(hepatosomatic index, HSI) and reproductive fitness
(gonadosomatic index [GSI], total oocyte count, and
oocyte mass). In 2015, measurements of head length, jaw
length, eye diameter, pectoral fin length, and pelvic
fin length were made on both sides of each fish for
assessing degree of FA. We observed declining condition
with fish size, increased liver size, and reduced
oocyte counts and oocyte size in female brook
stickleback in SFWR relative to those from Garvin
Brook. SFWR females had significantly higher FA than
Garvin females for all structures assessed, except
pelvic fin length. FA also was slightly higher for all
structures in SFWR males compared to Garvin males, but
differences were not significant. A composite FA index
combining all measurements from an individual fish into
a single value displayed highly significant differences
for female fish (SFWR FA {$ > $$ >$ } Garvin FA), but
not for male fish (SFWR FA = Garvin FA). Exposure of
brook stickleback to reduced water quality in SFWR
during early development appears to increase
morphological asymmetry in female (but not male) fish,
and continuing exposure to compromised water quality
throughout life impacts both general condition and
reproductive fitness of stickleback, especially older
female fish, in SFWR.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zuluaga-Hernandez:2023:NCI,
author = "Christian David Zuluaga-Hern{\'a}ndez and Carlos A.
Hincapi{\'e} and Marisol Osorio",
title = "Non-Conventional Ingredients for Tilapia
(\bioname{Oreochromis} spp.) Feed: a Systematic
Review",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "556",
day = "18",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110556",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/556",
abstract = "The objective of this systematic review was to
identify and classify, from the available literature,
non-conventional feed ingredients from terrestrial
plants, animals, algae, and fungi which have been
evaluated for their potential use for tilapia
(Oreochromis spp.) production. For this purpose, 795
papers published in the Scopus and Web of Science
databases between 2011 and 2021 were analyzed using the
Preferred Reporting Items for Systematic Reviews and
Meta-Analyses (PRISMA) methodology. Data on the growth
rate (GR) and effects on weight gain (WG), specific
growth rate (SGR) and feed conversion ratio (FCR);
digestibility; fatty acid profile (FAP) of the fish
carcass; and the survival rate (SR) were compiled in
databases and summary tables. The results were refined
according to different criteria, obtaining 144
documents that were pertinent for an in-depth analysis.
From those, we found that 50.7\% evaluated terrestrial
plants, 22.2\% animals, 13.9\% algae, 9\% fungi, and
the remaining, combinations of some of the above
categories. From the summarized results we concluded
that most of the non-conventional sources analyzed have
a positive potential impact as alternatives for
producing tilapia. Survival was the most evaluated
parameter, while digestibility was the least evaluated
parameter.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zheng:2023:ESD,
author = "Jishu Zheng and Zhengxi Wang and Decheng Pu and
Peiyuan Li and Xiuli Wei and Mai Li and Dongsheng Li
and Lihong Gao and Xuliang Zhai",
title = "Effects of Stocking Density on Intestinal Health of
Juvenile \bioname{Micropterus salmoides} in Industrial
Aquaponics",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "555",
day = "17",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110555",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/555",
abstract = "Stocking density is a key factor affecting the health
of aquatic organisms in industrial aquaponics. In this
study, Micropterus salmoides were assigned to one of
two density groups (160 and 120 fish/m 3) for 40 days.
The growth performance, intestinal morphology, enzyme
activity, and microbial communities were compared
between the two density groups. The findings revealed
that the higher stocking density condition exhibited an
increased weight gain rate and specific growth rate
during the developmental phase of the juvenile M.
salmoides. Moreover, remarkable increases in villi
height, villi width, and muscular layer thickness were
observed. Additionally, this elevated stocking density
condition also enhanced the activity of intestinal
antioxidant enzymes, consequently improving the
structural integrity of the intestine and augmenting
the digestive and absorptive capacities of the juvenile
M. salmoides. With regard to the intestinal microbial
community, the dominant phyla detected were Firmicutes
and Proteobacteria. However, under the higher stocking
density condition, there was a significant upsurge in
the abundance of Mycoplasma. Consequently, it is
advised to mitigate the abundance of such pathogenic
microorganisms through the regulation of the water
environment during the aquacultural process. Drawing
from recent investigations on the impact of various
factors on the intestinal microbiota of M. salmoides,
it can be deduced that the composition of the
intestinal microbiota is closely intertwined with
factors including aquaculture practices, feed
composition, water environment, and developmental
stage. In summary, the aforementioned research findings
possess noteworthy implications for the control of
stocking density in the cultivation of juvenile M.
salmoides.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mazzucato:2023:OBM,
author = "Matteo Mazzucato and Tiziano Dorotea and Eleonora
Franzago and Paolo Mulatti and Giulio Marchetti and
Claudia Casarotto and Andrea Fabris and Nicola
Ferr{\`e} and Anna Toffan and Andrea Marsella and
Azzurra Callegaro and Grazia Manca and Manuela {Dalla
Pozza}",
title = "Overview on the Biosecurity Measures of Salmonid Fish
Farms: a Case Study in \geoname{Italy}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "554",
day = "16",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110554",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/554",
abstract = "Italy is one of the main European producers of trout
fish, and the most important producing area is the
Autonomous Province of Trento (APT) in the North East.
The objective of the study was to obtain a snapshot of
the trout farms of the APT by identifying biosecurity
factors, objectifying them, and classifying the
establishments accordingly. Data from 62 salmonid farms
were collected using a national checklist in which
biosecurity elements were identified and assessed using
the method of expert elicitation. The purpose was to
evaluate the levels of biosecurity of a trout farm in
order to rank the risk of introduction and spread of
infectious fish diseases. The main critical factors
identified during this study were as following: (i)
cleaning and disinfection of the cargo truck; (ii)
regular collection, storage, and disposal of dead fish;
(iii) presence of anti-bird nets; (iv) use of dedicated
equipment for the different sectors of the farm; and
(v) presence of external areas dedicated to the loading
of dead fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kropielnicka-Kruk:2023:EFT,
author = "Katarzyna Kropielnicka-Kruk and Quinn P. Fitzgibbon
and Mohamed B. Codabaccus and Andrew J. Trotter and
Chris G. Carter and Gregory G. Smith",
title = "Effect of Feed Texture and Dimensions, on Feed Waste
Type and Feeding Efficiency in Juvenile
\bioname{Sagmariasus verreauxi}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "553",
day = "16",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110553",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/553",
abstract = "The ``messy'' feeding behaviour of spiny lobsters
remains an obstacle for formulated feed development.
This study examined the relationship between feeding
efficiency and feed waste by juvenile spiny lobster,
Sagmariasus verreauxi, fed different formulated pellet
diameters or lengths across two separate experiments.
Feed texture (hard and dry pellet, HDP; soft and moist
pellet, SMP) was also examined. Juvenile lobsters were
fed experimental feeds at 0.5\% BW daily over a 6 h
duration. The resulting feed waste was categorised as
either feeding-related waste (FRW) or
non-feeding-related waste (NFRW). For all feed types,
the FRW increased with increasing pellet diameter and
pellet length. The increase in FRW corresponded with a
decrease in NFRW, particularly for HDP, resulting in no
difference in total feed waste in any treatment
investigated. Thus, even with improved feeding
efficiency with small feed dimensions, feed intake was
not improved. Feed leaching rate decreased with
increasing pellet size, suggesting a more rapid decline
in feed attractiveness for smaller pellets. This
finding indicates that currently a counteractive
interaction exists between pellet size and feed
attractiveness and suggests improving attractiveness
would further enhance feeding. Future research should
aim at optimising feed dimensions simultaneously to
support efficient feeding whilst enhancing
attraction/gustatory stimulations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Monahan:2023:SPF,
author = "Dylan Monahan and Jeff S. Wesner and Stephanie M.
Parker and Hannah Schartel",
title = "Spatial Patterns in Fish Assemblages across the
{National Ecological Observation Network (NEON)}: the
First Six Years",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "552",
day = "16",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110552",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See correction \cite{Monahan:2024:CMA}.",
URL = "https://www.mdpi.com/2410-3888/8/11/552",
abstract = "The National Ecological Observation Network (NEON) is
a thirty-year, open-source, continental-scale
ecological observation platform. The objective of the
NEON project is to provide data to facilitate the
understanding and forecasting of the ecological impacts
of anthropogenic change at a continental scale. Fish
are sentinel taxa in freshwater systems, and the NEON
has been sampling and collecting fish assemblage data
at wadable stream sites for six years. One to two NEON
wadable stream sites are located in sixteen domains
from Alaska to Puerto Rico. The goal of site selection
was that sites represent local conditions but with the
intention that site data be analyzed at a continental
observatory level. Site selection did not include fish
assemblage criteria. Without using fish assemblage
criteria, anomalies in fish assemblages at the site
level may skew the expected spatial patterns of North
American stream fish assemblages, thereby hindering
change detection in subsequent years. However, if NEON
stream sites are representative of the current spatial
distributions of North American stream fish
assemblages, we could expect to find the most diverse
sites in Atlantic drainages and the most depauperate
sites in Pacific drainages. Therefore, we calculated
the alpha and regional (beta) diversities of wadable
stream sites to highlight spatial patterns. As
expected, NEON sites followed predictable spatial
diversity patterns, which could facilitate future
change detection and attribution to changes in
environmental drivers, if any.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:GGC,
author = "Yichao Zhang and Siyong Qin and Qinghua Liu and Wenqi
Wang",
title = "Gonad and Germ Cell Development and Maturation
Characteristics of the Pot-Bellied Seahorse
(\bioname{Hippocampus abdominalis}) under Captive
Breeding Conditions in \geoname{Northern China}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "551",
day = "15",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110551",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/551",
abstract = "Ovoviviparity and male pregnancy represent distinctive
reproductive strategies in seahorses. However, the
detailed process of gonadal development in seahorses,
particularly in the pot-bellied seahorse (Hippocampus
abdominalis), remains largely unknown. In this study,
we investigated the complete gonadal development
process of the pot-bellied seahorse under captive
breeding conditions (18 {\pm} 1 {$^\circ $}C).
Immediately after birth, primordial germ cells (PGCs)
were found within the genital ridge, enclosed by a
single layer of somatic cells. Around 7--9 days after
birth (DAB), the ovary begins to differentiate. By 30
DAB, two germinal ridges had formed along the edge of
the follicular lamina in the ovary. The primary
oocytes, resulting from this differentiation process,
gradually migrated from the dorsal sides to the
mid-ventral area of the ovary, eventually maturing into
eggs. In the testis, the primary and secondary
spermatocytes appeared at 15 and 30 DAB, respectively,
preceding the formation of the testicular lumen (50
DAB). The testis was observed to consist of a single
large germinal compartment. Under captive breeding
conditions in Northern China, the pot-bellied seahorse
demonstrated year-round breeding capability, with each
male producing approximately 100--150 larvae. The
findings from this study contribute valuable insights
into seahorse aquaculture and enhance understanding of
the unique reproductive strategy employed by
seahorses.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{An:2023:MCE,
author = "Hyung-Eun An and Min-Ho Mun and Chang-Bae Kim",
title = "Metabarcoding by Combining Environmental {DNA} with
Environmental {RNA} to Monitor Fish Species in the
\geoname{Han River, Korea}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "550",
day = "15",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110550",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/550",
abstract = "Fishes are ecologically important organisms that have
long lifespans, high mobilities, and diverse trophic
levels. Due to their importance, fishes are used as
bioindicators for monitoring aquatic environments. One
method for monitoring fishes is based on environmental
DNA (eDNA), which are the deoxynucleic acids released
by organisms into the environment. However, there has
been a problem with false positives because eDNA is
relatively stable in the environment and could even
likely represent dead or non-inhabiting organisms. To
address this weakness, environmental RNA (eRNA), which
degrades more rapidly than eDNA in the environment, can
be utilized to complement eDNA. But, to date, few
studies have used eRNA for freshwater fish monitoring.
In this study, to determine the relative usefulness of
eDNA and eRNA metabarcoding in freshwater fishes, we
performed eDNA and eRNA metabarcoding on 12S rRNA
targeting fish using water samples that were collected
from three locations in the Han River. We then
calculated the sensitivity and positive predictivity of
this approach by comparing our data to the previous
specimen capture survey (PSCS) data from the last six
years. The results showed that 42 species were detected
by eDNA and 19 by eRNA at the three locations. At all
locations, compared to the PSCS data, the average
sensitivity was higher for eDNA (46.1\%) than for eRNA
(34.6\%), and the average positive predictivity was
higher for eRNA (31.7\%) than for eDNA (20.7\%). This
confirmed that eDNA metabarcoding has the advantage of
broadly determining species presence or absence
(including those that are no longer present or dead),
but it also generates false positives; meanwhile, eRNA
metabarcoding reports living fish species, but detects
fewer species than eDNA. Combining eDNA and eRNA
therefore emphasizes their advantages and compensates
for their disadvantages, and conducting this may
therefore be useful for identifying false positives and
monitoring the fish species that are actually present
in the environment. This metabarcoding technique can be
used in the future to provide insights into the aquatic
environment and the monitoring of fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:TAG,
author = "Yifan Zhang and Dandan Zhang and Ying Tian and Junxia
Mao and Yang Liu and Zhenlin Hao",
title = "Transcriptome Analysis of Gill Tissues from
\bioname{Neptunea cumingii} in Different Seasons",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "549",
day = "11",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110549",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/549",
abstract = "Neptunea cumingii is an economically important marine
shellfish found in the Yellow and Bohai Seas areas of
China. In this study, samples of Neptunea cumingii were
collected in Zhangzidao and Yantai during spring,
summer, autumn, and winter to clarify the gene
expression patterns and regulatory mechanisms in their
gills in different seasons. Transcriptome analysis was
conducted using Neptunea cumingii gill tissues, and
genes with significantly different expression levels
were extracted for functional verification. The most
genes with differences in expression (DEGs) were found
in comparisons of the winter and summer samples. Gene
enrichment analysis based on Gene Ontology and Kyoto
Encyclopedia of Genes and Genomes terms showed that
these DEGs were mainly involved in immune and metabolic
pathways, and they had significant effects on oxidative
stress, body metabolism, and protein synthesis in
Neptunea cumingii. Further screening of DEGs identified
thirty-four genes related to temperature regulation,
comprising thirteen genes with roles in innate immunity
in shellfish, twelve genes related to oxidative stress,
and nine genes related to protein synthesis and energy
metabolism. Eleven DEGs were randomly selected for qPCR
verification, and the results were consistent with the
transcriptome analysis results. In summary, the
transcriptome results differed significantly between
seasons in the gill tissues of Neptunea cumingii. The
expression levels of immune regulatory genes could be
promoted in Neptunea cumingii during the high
temperature season, whereas the expression of these
genes may be inhibited in the low temperature season.
The results obtained in this study provide insights
into the molecular defense mechanisms that might allow
Neptunea cumingii to adapt to climate change.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Miller:2023:YAH,
author = "Michael J. Miller",
title = "43 Years after {H. G. Moser}'s Seminal
``{{\booktitle{Morphological and Functional Aspects of
Marine Fish Larvae}}}'': The Commonalities of
Leptocephali and Larvae of Other Marine Teleosts",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "548",
day = "10",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110548",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/548",
abstract = "H.G. Moser was not particularly well known regarding
the Elopomorpha, but he began his seminal 1981 fish
larvae morphology review by describing the mysteries of
European eels and their leptocephali, and he eventually
described some California Current leptocephali.
Descriptions of all types of leptocephali and then
microscope photographs have revealed complex
pigmentation patterns and diverse body shapes that
parallel the features of the other teleost fish larvae
that his review showed so clearly, some of which appear
to mimic gelatinous zooplankton (GZ). Most recently,
underwater photographs and video of leptocephali
indicate that predator avoidance through transparency,
shape-change-mediated mimicry, or morphological
features have evolved in response to natural selection
from predation in ways generally similar to other fish
larvae. Several families of leptocephali perform
remarkable curling into round or flattened shapes or
use expandable chromatophores when threatened, which
appears to mimic GZ for predation reduction. Like a
number of other teleost fish larvae, some leptocephali
have body extensions or telescopic eyes, and at least
one has a long caudal filament with pigmented swellings
that is similar to the remarkable GZ mimicry appendages
of some fish larvae. The morphology and use of mimicry
of leptocephali in comparison to other fish larvae is
reviewed as a tribute to H.G. Moser's remarkable
career.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wildhaber:2023:RGC,
author = "Mark L. Wildhaber and Benjamin M. West and Karlie K.
Ditter and Adrian P. Moore and Alex S. Peterson",
title = "A Review of Grass Carp and Related Species Literature
on Diet, Behavior, Toxicology, and Physiology Focused
on Informing Development of Controls for Invasive Grass
Carp Populations in \geoname{North America}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "547",
day = "10",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110547",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/547",
abstract = "Grass carp (Ctenopharyngodon idella) are globally
important in aquaculture and aquatic vegetation
control. However, escaped grass carp have established
invasive populations. A targeted keyword search was
performed on a carp (order: Cypriniformes) literature
database maintained by the U.S. Geological Survey to
identify literature relevant to grass carp. Additional
sources cited in reviewed documents and provided by
numerous reviewers were also included. There were three
focus areas designed to provide support for invasive
grass carp management: (1) diet and behavior; (2)
physiological constraints, toxicity, and biology; and
(3) gut physiology. Each focus area provides
information to guide development of potential pathways
for invasive grass carp control. Information from other
carp species was used to fill in gaps where grass carp
information was lacking and provide additional,
potential research directions. Diet-related information
included food selection and aquacultural diet
formulations. Behavioral information included stimuli
and non-physical barriers to attract, repel, or stop
movement. Physiological constraints, toxicology,
reproductive control, and biological control provide a
research review for control options. Gut physiology and
related control pathways provide knowledge to improve
toxin or pathogen delivery. This review provides a
basis for developing approaches and research for
controlling invasive grass carp populations,
aquaculture, and native population management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mendez-Martinez:2023:GPF,
author = "Yuniel M{\'e}ndez-Mart{\'\i}nez and Alan Rodrigo
Vera-Veliz and Edilmar Cort{\'e}s-Jacinto and Yanis
Cruz-Quintana and Aroldo Botello-Leon and Pedro Daniel
Mendoza-Carranza and Natalia S. Calvo",
title = "Growth Performance, Feed Utilisation, Digestive and
Metabolic Enzyme Activity, and Liver Morphohistology in
Hybrid Tilapia (\bioname{Oreochromis mossambicus} $
\times $ \bioname{Oreochromis niloticus}) Juveniles Fed
with the Inclusion of Chitosan in Their Diet",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "546",
day = "09",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110546",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/546",
abstract = "This study aimed to evaluate the growth performance,
feed utilisation, digestive and metabolic enzyme
activity, and liver histology in juveniles of hybrid
red tilapia (Oreochromis mossambicus $ \times $
Oreochromis niloticus) fed with the inclusion of
chitosan in their diet. Six dietary chitosan levels (0
``control'', 10, 20, 30, 40, and 50 g kg {$-$1}) were
used to feed juvenile fish (initial weight 7.50 {\pm}
0.20 g) that were cultured for eight weeks in 18 tanks
at a density of 15 fish/tank. The growth performance
presented significant differences (p \SGMLentity{706}
0.05) for weight gain, specific growth rate, feed
conversion ratio, hepatosomatic index, and survival
rate. The digestive and metabolic enzyme activities
were significantly (p {$<$} 0.05) influenced by the
levels of chitosan in the administered diet.
Histologically, no damage was found in the liver;
however, morphometrically, a significant difference (p
{$<$} 0.05) was found in the hepatocyte area and
sinusoid area. Vacuolisation of hepatocytes was found
in treatments with 40 and 50 g kg {$-$1} of chitosan in
the diet. Treatments with doses of chitosan showed a
better response (p {$<$} 0.05) compared to the control
treatment in most of the different groups of variables
analysed. The result of the principal component
analysis suggests that a diet containing 40 g kg {$-$1}
of chitosan is optimal for tilapia growth
performance.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Franca:2023:CFR,
author = "Susana Fran{\c{c}}a",
title = "Changes in the Functional Role of the \geoname{Tejo
Estuary (Portugal, Europe)} According to Fish
Ecological Guilds",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "545",
day = "08",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110545",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/545",
abstract = "Estuaries are extremely productive ecosystems,
providing habitats for numerous aquatic species and
crucial ecological services. The Tejo estuary, one of
the largest European estuaries, has been thoroughly
studied, and its important functional role as a nursery
for several commercially important fish species is
already established. In the present work, a trait-based
approach was applied to functionally describe the fish
community structure of the Tejo estuary and to
enlighten potential changes in the ecosystem
functioning at this level, following environmental
changes expected to occur. To predict the distribution
of species from the two most representative ecological
guilds of the Tejo estuary, estuarine residents and
marine migrants, species distribution models were built
using an ensemble technique (combining forecasts of
single models). The predictions obtained were more
accurate for the marine migrants and the species
distribution was strongly related with salinity,
whereas estuarine species, were also influenced by
depth, habitat type and river flow. The potential
distributions of these ecological guilds showed that
marine migrants will tend to use upstream areas in the
estuary, where salinity is lower. Nonetheless, salinity
is expected to increase as extreme weather events such
as droughts tend to occur more frequently, decreasing
favorable habitat availability for these species, and
thus threatening the crucial role this ecosystem plays
for these species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2023:GDG,
author = "Zhe Zhang and Chengkuan Lu and Kebing Lin and Weiwei
You and Zhangwu Yang",
title = "Genetic Diversity and Genetic Structure among Four
Selected Strains of Whiteleg Shrimp
(\bioname{Litopenaeus vannamei}) Using {SSR} Markers",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "544",
day = "06",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110544",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/544",
abstract = "The whiteleg shrimp (Litopenaeus vannamei) is one of
the most economically important aquaculture species in
China. Genetic diversity is crucial for maintaining the
gene pool of farmed shrimp. In this study, the effects
of artificial selection on the genetic structure of
four whiteleg shrimp strains were evaluated using
microsatellite sequences. The results showed that the
observed heterozygosity (H o), expected heterozygosity
(H e), and the polymorphism information content (PIC)
of the four selected strains ranged from 0.446 to
0.574, 0.450 to 0.566, and 0.435 to 0.509,
respectively. All the selected strains maintained a
moderate level of genetic diversity. Most inbreeding
coefficients (F is) in the four strains were positive
but not significantly different from zero, indicating a
relatively low degree of inbreeding within each strain.
However, H e and PIC in line 5 showed a decreasing
trend from the 2017 to the 2019 generations, and F is
in line 10 showed a significant increasing trend across
generations, indicating that measures must be taken to
maintain the level of genetic diversity for lines 5 and
10. UPGMA cluster trees showed that the four breeding
lines had apparent genetic differences, which could
provide a genetic basis for studying crossbreeding
between selective lines and the utilization of
heterosis. This study will be useful for population
genetic research and the breeding strategies of
whiteleg shrimp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yu:2023:BAU,
author = "Young-Bin Yu and Jae-Ho Choi and Ju-Hyeong Lee and
A-Hyun Jo and Sung Won Han and Song-Hun Han and Hee Jae
Choi and Cheol Young Choi and Ju-Chan Kang and EunYoung
Min and Jun-Hwan Kim",
title = "Biofloc Application Using Aquaponics and Vertical
Aquaculture Technology in Aquaculture: Review",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "543",
day = "04",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110543",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/543",
abstract = "Some aspects of traditional aquaculture have negative
impacts on the aquatic environment, leading to
pollution and disease outbreaks in farmed organisms.
Biofloc technology (BFT) is a closed aquaculture system
that utilizes specific microbial communities to remove
ammonia emitted from aquaculture organisms or adds
carbon to the aquaculture system to improve water
quality. BFT has benefits, such as increasing
production and improving water quality, and reducing
disease spread and pollution, without the need for
water exchange. However, there are disadvantages, such
as rapid changes in water quality due to accumulation
of dissolved nutrients and total suspended soils (TSS)
and the requirement for expensive aeration equipment to
maintain dissolved oxygen. BFT can be enhanced in value
and efficiency by combining it with other aquaculture
technologies, such as aquaponics and vertical
aquaculture to overcome the disadvantages. The
integration of biofloc with technologies from the
fourth industrial revolution holds potential for
further development, while aquaponics and vertical
farming can eliminate geographical limitations and
accelerate the urbanization of aquaculture. The
integration of aquaponics and vertical aquaculture with
BFT has potential for development, accelerating the
urbanization of aquaculture and removing geographic
limitations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yildirim-Aksoy:2023:FBS,
author = "Mediha Y{\i}ld{\i}r{\i}m-Aksoy and Rashida Eljack and
Janset Aksoy and Benjamin H. Beck",
title = "Frass from Black Soldier Fly Larvae, \bioname{Hermetia
illucens}, as a Possible Functional Dietary Ingredient
in Channel Catfish Feed",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "542",
day = "02",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110542",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/542",
abstract = "Frass--the by-product of the larva meal industry--was
recently shown to be a sustainable ingredient for use
in diets of several fish species. Additionally, because
of its possible immunomodulatory activity and
anti-microbial properties, frass may have great
potential as an organic method of pathogen control in
aquaculture. Five diets containing 0, 5, 10, 20 and
30\% frass from black soldier fly larvae, Hermetia
illucens, were fed to channel catfish (5.24 {\pm} 0.04
g) in quadruplicate aquaria to apparent satiation twice
daily. At the end of the 10-week feeding trial, blood
samples were collected from all groups to measure
hematological and immune parameters, and to determine
the effects of dietary frass on resistance to
Flavobacterium covae infection. Hematological
parameters (red blood cell count, hemoglobin, and
hematocrit)---but not white blood cell count---were
improved with the inclusion of frass. Serum glucose
levels were significantly lower in fish on the diet
with frass than fish on the diet without frass. Fish
fed the highest dietary levels of frass (30\%) had a
significantly higher serum cholesterol level than fish
on the control diet. Serum complement activity was
significantly higher in fish on diets containing frass
at levels of 10\% and 20\%. No significant differences
were observed in other measure serum components
including albumin, alkaline phosphatase, alanine
aminotransferase, amylase, calcium, phosphorus,
potassium, total protein, globulin, thyroxine and
lysozyme activity. Even though overall mortality was
low (0--17\%), fish on the diets containing frass at
levels 20\% or more showed significantly higher
survival than that of control fish or fish on lower
levels of dietary frass. The use of frass in the
catfish diet may prove beneficial by improving
hematological parameters, and select serum immune
effectors, and the overall resistance of juvenile
channel catfish against F. covae infection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Madera-Santana:2023:DDC,
author = "Sara Madera-Santana and Carlos
Rodr{\'\i}guez-Garc{\'\i}a and Jairo
Castro-Guti{\'e}rrez and {\'A}ngel Rafael
Dom{\'\i}nguez-Bustos and Remedios Cabrera-Castro",
title = "Discarded but Not Dismissed: a Comprehensive Study of
the Feeding Habits of the Brown Comber
(\bioname{Serranus hepatus}, ({Linneaus} 1758)) in the
\geoname{Gulf of C{\'a}diz (NE Atlantic)}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "541",
day = "02",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110541",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/541",
abstract = "The brown comber (Serranus hepatus) is a small
benthopelagic species with no commercial value,
primarily caught by bottom trawls as a by-catch. In
this work, we studied the feeding habits of this
species. For this purpose, samples were obtained from
the trawl fleet within the different editions of the
ECOFISH project carried out between 2019 and 2022. A
total of 1534 individuals were analyzed. In the diet
analysis, various factors were considered, such as the
season, the depth, and the time of day of the capture,
as well as the size range of the individuals caught.
For the feeding analysis, different indexes were
calculated, such as the vacuity index (\%Vi) and index
of relative importance (\%IRI). The size range of the
specimens was between 3.2--16.3 cm, and the weight was
between 1.02--39.73 g. Of the stomach content analyzed,
49.7\% of the stomachs were found to be empty. The
resources with the greatest importance in the diet of
the brown comber were from the crustacean group,
especially mysidaceans and decapods. There were
differences in the diet according to season, depth, and
size; however, there was no variation in diet by the
time of day.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sun:2023:BTF,
author = "Yan Sun and Yi Huang and Ying Wang and Yanqun Wang and
Guiying Hao and Changwei Jiang and Zhiqiu Huang",
title = "The {bZIP} Transcription Factor Family Orchestrates
the Molecular Response to Nitrite Stress in the
Largemouth Bass Spleen",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "540",
day = "01",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110540",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/540",
abstract = "Nitrite toxicity poses a significant threat to aquatic
organisms, including largemouth bass (LMB) and
Micropterus salmoides. This study aimed to elucidate
the role of bZIP transcription factors in mediating the
molecular responses to nitrite stress in the LMB
spleen. We identified 120 bZIP genes in the LMB genome
using bioinformatics analysis and divided them into 11
subgroups based on phylogenetic relationships. Under
nitrite stress, the bZIP_XI subgroup was upregulated,
suggesting the activation of the stress response in the
LMB spleen. Cellular pathway analysis revealed
enrichment of pathways related to stress response, DNA
repair, apoptosis, and autophagy. Co-expression network
analysis highlighted bZIP_XI members such as
msabZIP_49, msabZIP_12, msabZIP_39, and msabZIP_116 as
potential key regulators. These transcription factors
likely modulated the expression of stress-related genes
like VCAM1, POLE3, and BMP1. Conserved binding motifs
in the promoters of these genes may support regulation
by bZIP_XI. Furthermore, bZIP_XI members correlated
with immune cell infiltration in the spleen,
potentially regulating immune-related genes like BCL2L1
and SELE. Homologs of bZIP_XI in other fish species
exhibited similar expression patterns under stress.
Overall, this study implicates the bZIP transcription
factor family, notably the bZIP_XI subgroup, in
orchestrating the molecular response of the LMB spleen
to nitrite toxicity by regulating stress response
pathways and immune function. These findings provide
insights into nitrite stress adaptation in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Osowski:2023:DHH,
author = "Ana R. Osowski and Matthew B. Jargowsky and Pearce T.
Cooper and Sean P. Powers and J. Marcus Drymon",
title = "Dietary Habits of Hardhead (\bioname{Ariopsis felis})
and Gafftopsail (\bioname{Bagre marinus}) Catfish
Revealed through {DNA} Barcoding of Stomach Contents",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "539",
day = "31",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110539",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/539",
abstract = "A better understanding of trophic interactions between
hardhead catfish (Ariopsis felis) and gafftopsail
catfish (Bagre marinus) is crucial for developing
multi-species management strategies for the northern
Gulf of Mexico (GOM). These two species are often
aggregated in food web models; however, limited data
are available to substantiate this approach. Therefore,
the present study aimed to describe the dietary habits
of hardhead catfish and gafftopsail catfish using
analysis of stomach contents aided by DNA barcoding.
Hardhead (n = 693) and gafftopsail (n = 655) catfish
were sampled in the northern GOM from 2015--2019 using
both fisheries-dependent and -independent techniques.
The average percent number (\%N), average percent mass
(\%M), prey specific number (\%PN), prey specific mass
(\%PM), and prey-specific index of relative importance
(\%PSIRI) were computed to quantify prey species. The
stomach content analysis identified distinct
differences in diet between hardhead and gafftopsail
catfish. Crustaceans were the most important prey for
hardhead catfish, while gafftopsail catfish showed a
significantly broader dietary breadth and were
primarily piscivorous. Multivariate analyses indicated
that the location of capture explained the greatest
amount of diet variability for both species. These
findings address fundamental knowledge gaps regarding
the dietary habits of hardhead and gafftopsail catfish
in northern GOM ecosystems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shahraki:2023:DEA,
author = "Mojgan Zare Shahraki and Yazdan Keivany and Eisa
Ebrahimi Dorche and Karen Blocksom and Andreas Bruder
and Joseph Flotemersch and Doru B{\u{a}}n{\u{a}}duc",
title = "Distribution and Expansion of Alien Fish Species in
the \geoname{Karun River Basin, Iran}",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "538",
day = "31",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110538",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/538",
abstract = "We assessed the distribution of alien fishes in the
Karun River Basin, Iran. Fish were collected from 39
sites during the November--December 2018 low-flow
period. In total, 39 fish species from nine orders and
14 families were documented. Among these, 10 species
were alien to the basin (986 individuals; 15.7\%). Four
species were the most abundant alien species and
primarily in impounded, downstream reaches. Redundancy
analysis (RDA) was conducted to identify the extent of
changes in alien fish assemblages with environmental
parameters. RDA1 and RDA2 accounted for 36.24\% and
25.33\% of the variation of alien species,
respectively. Altitude, depth, electrical conductivity,
water temperature, turbidity, dissolved oxygen, and
river width were the most significant parameters
affecting alien species distributions. We present a
dual-pathway cause-and-effect hypothesis proposing that
alien fish species presence causes declines in the
ecological status of native fish communities. We then
explore how human-induced aquatic ecosystem degradation
creates opportunities for alien species to invade new
ecosystems, further impacting native fish communities.
Our study contributes insight into the cause and effect
of the presence of alien fish species in the Karun
River Basin and emphasizes the urgency of conservation
measures to protect this critically endangered
watershed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:RLR,
author = "Chang Xu and Yang Liu and Zhaobin Pei",
title = "Research on Legal Risk Identification, Causes and
Remedies for Prevention and Control in
\geoname{China}'s Aquaculture Industry",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "537",
day = "29",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110537",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/537",
abstract = "Aquatic products fulfill the protein needs of people
and play an important role in food safety. And
aquaculture is prized for its high productivity,
sustainability and environmental friendliness.
Considering the importance of aquaculture, the legal
risks exposed during the aquaculture process deserve
attention in order to prevent them from hindering the
development of the aquaculture industry. Through online
research, literature analysis and practical
communication, it is shown that the current legal risks
with commonalities include land use violations, lack of
legal documents, failure to meet tailing water
criteria, unquarantined fry and misuse of prohibited
agricultural pharmaceuticals through online research,
literature analysis and practical communication. By
analyzing the reasons for the formation of legal risks
and combining the experiences in sustainable
development of three major aquaculture countries, which
are Korea, Norway and Chile, this paper provides
targeted preventive remedies and suggestions for
aquaculture operators, administrative parties,
legislators and other parties on legal risks. It
includes promoting the improvement of the rule of law
in multiple aspects, clarifying the positioning of the
aquatic breeding certificates, improving and
propagating the standards for wastewater discharge,
increasing the self-sufficiency rate of aquatic fry and
fingerlings, as well as making use of the synergy of
soft law and hard law.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Boussard:2023:NSS,
author = "Annika Boussard and Stephanie Edlund and Stephanie
Fong and David Wheatcroft and Niclas Kolm",
title = "No Sex-Specific Effects of Artificial Selection for
Relative Telencephalon Size during Detour Learning and
Spatial Discrimination in Guppies (\bioname{Poecilia
reticulata})",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "536",
day = "26",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110536",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/536",
abstract = "Over recent decades, substantial research has focused
on fish cognitive evolution to increase our
understanding of the evolution of the enormous
diversity of cognitive abilities that exists in fishes.
One important but understudied aspect of cognitive
evolution is sexual dimorphism in cognitive abilities.
Sex-specific variation in brain region morphology has
been proposed to be an important mechanism in this
context. However, it is also common to find
sex-specific variation in behavior and cognition
without associated differences in brain morphology
among the sexes. The telencephalon is the major
cognitive center in the vertebrate brain and variation
in telencephalon size has been associated with
variation in cognition. Here, we utilize recently
developed guppy artificial selection lines with ca.
10\% differences in relative telencephalon size to
investigate whether similar responses to selection of
the size of this region may affect cognitive abilities
differently in males and females. To that end, we
compared two ecologically relevant aspects of
cognition, detour learning and binary spatial
discrimination. We tested the significance of the
interaction between telencephalon size and sex, and we
found no sex-specific effects of evolutionary increases
in telencephalon size in the cognitive abilities
tested. This study indicates that no clear cognitive
sex-specific effects occur in response to rapid
selection of telencephalon size. We suggest that future
research on sexual dimorphism in cognitive abilities in
fish could use various cognitive tests and examine
telencephalic sub-regions to gain a more comprehensive
understanding of their evolution.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2023:EDL,
author = "Yongqiang Liu and Enhao Huang and Yi Xie and Liuqing
Meng and Dongsheng Liu and Ziqi Zhang and Jiang Zhou
and Qin Zhang and Tong Tong",
title = "The Effect of Dietary Lipid Supplementation on the
Serum Biochemistry, Antioxidant Responses, Initial
Immunity, and {mTOR} Pathway of Juvenile Tilapia
(\bioname{Oreochromis niloticus})",
journal = j-FISHES,
volume = "8",
number = "11",
pages = "535",
day = "26",
month = oct,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8110535",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/11/535",
abstract = "The objectives of this study were to investigate the
effect of different dietary lipid levels on the serum
biochemistry, antioxidant responses, initial immunity,
and mTOR pathway of juvenile genetic improvement-farmed
tilapia (GIFT, Oreochromis niloticus). Six groups of
the juveniles (initial body weight 20.66 {\pm} 1.33 g)
in triplicate were fed for 90 days using six
iso-nitrogen diets with different lipid levels (0.35\%,
3.35\%, 6.35\%, 9.35\%, 12.35\%, and 15.35\%). The main
results were as follows: Compared with the control
group (0.35\%), the diets with different lipid
supplementation significantly improved (p {$<$} 0.05)
the contents of total protein (TP), albumin (ALB),
globulin (GLB), glucose (GLU), triglyceride (TG), total
cholesterol (T-CHO), high-density lipoprotein (HDL),
low-density lipoprotein (LDL), total antioxidant
capacity (T-AOC), malondialdehyde (MDA), complement 3
(C3), and immunoglobulin M (IgM), the activities of
superoxide dismutase (SOD), glutathione peroxidase
(GSH-Px), catalase (CAT), lysozyme (LYZ), and alkaline
phosphatase (ALP), the expression level of
phosphatidylinositol-3-kinase (PI3K), Akt protein
kinase B (Akt), and mammalian target of rapamycin
(mTOR) genes in juvenile tilapia. However, diets with
different lipid supplementation significantly reduced
(p {$<$} 0.05) the expression level of tumor necrosis
factor {\alpha} (TNF-{\alpha}), interleukin-1{\beta}
(IL-1{\beta}), and interleukin-6 (IL-6) genes of
juvenile tilapia. In conclusion, the with different
lipid supplementation could significantly affect the
serum biochemistry, antioxidant responses, initial
immunity, and mTOR pathway of juvenile tilapia.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2023:CGA,
author = "Yuanhao Yang and Yameng Zhang and Fenggang Li and Sien
Wen and Lvzhou Wang and Guozhu Lan and Jishu Zhou and
Yang Li and Zilin Zhu",
title = "Comparative Genomic Analysis Unveils Potential Factors
Contributing to the Endangerment of \bioname{Silurus
lanzhouensis}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "613",
day = "18",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120613",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/613",
abstract = "Silurus lanzhouensis is a large endemic fish
exclusively found in the middle and upper reaches of
China's Yellow River, with a restricted distribution
range and a small population size and is listed as an
endangered species. To investigate the potential
genetic factors contributing to the observed decrease
in the population of S. lanzhouensis, we have
successfully assembled the first chromosome-level
genome of S. lanzhouensis. This genome comprises a
total of 30 chromosomes, with a size of 794.43 Mb, and
23,093 protein-coding genes. Comparative genomics
analysis reveals that the genetic relationship between
S. lanzhouensis and S. meridionalis Chen is the
closest, and their common ancestor experienced two
rounds of whole genome duplication (WGD) before
diverging. Notably, amino acid metabolism-related
pathways are enriched in the unique S. lanzhouensis
gene family. In addition, we observed that the
chemokine receptor and scavenger receptor gene families
contracted in S. lanzhouensis, which may be associated
with its diminished immune response. Our research
findings provide valuable insights into the endangered
mechanism of S. lanzhouensis and enhance our
understanding of the evolution of Siluriformes fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Piper:2023:EAI,
author = "Adam T. Piper and Paula J. Rosewarne and Charlotte
Pike and Rosalind M. Wright",
title = "The Eel Ascending: The Influence of Lateral Slope,
Climbing Substrate and Flow Rate on Eel Pass
Performance",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "612",
day = "18",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120612",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/612",
abstract = "Optimising the design of passage facilities to restore
fluvial connectivity for juvenile European eel
(Anguilla anguilla) is a key priority within
conservation efforts for the species, across the
majority of its freshwater range. Employing an
experimental setup that simulated gravity-fed upstream
eel passes, this study demonstrated that novel
V-profile passes, which incorporate two lateral slopes
({15$^\circ $}), performed better than laterally flat
passes over the flow rates tested (0.2--0.6 L s
{$-$1}). For the small eel size used (60--80 mm
length), the bristle substrate consistently
outperformed studs, but the lateral slope had a greater
effect on passage metrics than the substrate choice.
Our findings strongly support the use of V-shaped
channels for upstream migrating eel at fish passage
facilities, particularly in scenarios where flow rates
may be elevated and/or fluctuating, such as for
gravity-fed passes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Peele:2023:EIT,
author = "Emily E. Peele and Charlie Huveneers and Culum Brown
and Connor R. Gervais and Kara E. Yopak",
title = "Effects of Increased Temperature on Brain and Sensory
Development in the \geoname{Port Jackson} Shark
(\bioname{Heterodontus portusjacksoni})",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "611",
day = "17",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120611",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/611",
abstract = "Morphological differences in the peripheral (sensory)
and central (brain) nervous system may confer sensory
and/or behavioral variation in elasmobranchs, both
across taxa and throughout ontogeny. Over the last
century, sea surface temperatures have increased over
0.5 {$^\circ $}C and are predicted to rise 1--4
{$^\circ $}C by the year 2100, potentially affecting
species' physiological performance negatively. As the
nervous system of fishes grows continually throughout
their lives, it may be highly plastic in response to
environmental changes. This study examined the effects
of increased rearing temperature on nervous system
development in Port Jackson sharks (Heterodontus
portusjacksoni). Egg cases (n = 21) were collected from
Gulf St. Vincent (Adelaide, SA) and placed into either
ambient (17.6 {$^\circ $}C) or 3 {$^\circ $}C above
ambient seawater conditions through hatching and reared
for up to five months post-hatch. Relative volumes of
the eyes and nose (olfactory rosette) were quantified
using magnetic resonance imaging, and relative brain
size and size of major brain regions were compared
between the two treatment groups. The size of the
olfactory bulbs and tegmentum varied significantly
between the treatment groups, which suggest differences
in primary, secondary, or tertiary sensory processing
and/or motor functions at elevated temperatures. While
studies on acute responses to environmental conditions
cannot inform true adaptation across broad timescales,
understanding the effects of increased temperature on
the brain phenotype can aid in predicting how
elasmobranchs may fare in response to changing ocean
conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huang:2023:BAH,
author = "Po-Lin Huang and Farok Afero and Yao Chang and Bo-Ying
Chen and Hsun-Yu Lan and Yen-Lung Hou and Cheng-Ting
Huang",
title = "The Bioeconomic Analysis of Hybrid Giant Grouper
(\bioname{Epinephelus fuscoguttatus} $ \times $
\bioname{Epinephelus lanceolatus}) and Green Grouper
(\bioname{Epinephelus malabaricus}): a Case Study in
\geoname{Taiwan}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "610",
day = "17",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120610",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/610",
abstract = "Grouper aquaculture is a rapidly growing and dynamic
industry within the Taiwan aquaculture sector that
serves as an important source of income generation.
Farming fishes at a better location (FFABL) is a
promising production strategy. This study evaluated the
production and economic performance of grouper farming
in Taiwan. This investigation employed two steps: (1) a
cost--benefit analysis to determine the main component
affecting profitability; and (2) multivariate
statistics to clarify the relationship between
biological and economic factors. The findings suggest
that the best choice of species is hybrid giant grouper
that generates and highlights a positive income, viable
benefit--cost ratio and profit rate. The study found
the feed management of hybrid giant groupers requires
more consideration due to its low feed conversion
ratio. The producers need to replace trash fish in
commercial feed during the production cycle to ensure
better feed management. Finally, this paper argues that
feed is the main constraint for grouper farming, which
is sensitive to any change in feed productivity, and
this constraint must be solved to sustain the grouper
industry in Taiwan.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jensen:2023:ITF,
author = "Lasse Lange Jensen and Thomas Bj{\o}rn and Andreas
Hein Korsgaard and Cino Pertoldi and Niels Madsen",
title = "Influence of Turbidity on Foraging Behaviour in
Three-Spined Sticklebacks (\bioname{Gasterosteus
aculeatus})",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "609",
day = "16",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120609",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/609",
abstract = "Anthropogenic activities increase turbidity in coastal
marine environments globally, and turbidity is
particularly caused by eutrophication. Turbidity is a
measurement of the scattering and absorption of light
by suspended matter in water. An increase in turbidity
influences visual predators and affects community
structures and whole ecosystems. The three-spined
stickleback (Gasterosteus aculeatus) is a widespread
species in the northern hemispheric Pacific and
Atlantic oceans. It is a visual predator and,
therefore, a very well-suited species for studying the
effects of increasing turbidity on foraging behaviour
and activity. Sticklebacks used for this study were
from an aquarium in the North Sea Oceanarium. They have
been in the aquarium for around two months and were
originally collected in a highly eutrophicated marine
fjord system. They were individually placed in an
observation aquarium, fed with krill, given 10 min to
forage, and observed by video cameras. The video films
were analysed to study stickleback predation behaviour.
Experiments were repeated with four different turbidity
treatments, ranging from a mean of 0.034 up to 10 NTU
(nephelometric turbidity unit). Bentonite clay was used
as a turbidity-increasing substance. A statistically
significant difference in foraging behaviour and
activity between the turbidity treatments was observed.
The test subjects were found to lunge less for prey and
had a higher feeding latency with increasing turbidity.
Additionally, they were less active with increasing
turbidity. The behavioural instability estimated as a
variation in feeding latency increased with increasing
turbidity but decreased at the highest turbidity value.
Our study indicates an effect of turbidity-increasing
events on the behaviour of the three-spined stickleback
and potentially also other similar visual predators.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cai:2023:ERL,
author = "Xin Cai and Yaxing Zhang and Bin Wang and Aijun Cui
and Yan Jiang and Zhaojun Meng and Yuting Li and
Yongjiang Xu",
title = "Effects of Recombinant Leptin Proteins on the
Expression of Key Genes in the {HPG} Axis and Liver of
Tongue Sole In Vitro",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "608",
day = "16",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120608",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/608",
abstract = "Leptin (Lep) plays a crucial role in controlling food
intake and maintaining energy balance in mammals. While
several studies have shown the presence of multiple
leptin genes in teleosts, limited information is
currently available on how sex steroid hormones
regulate the expression of these genes in fish. In this
study, we used two previously expressed and purified
leptin proteins to incubate four tissues in vitro
(hypothalamic-pituitary-gonadal axis and liver) and
used the RT-qPCR method to detect the expression of
genes related to growth and reproduction in tongue sole
(Cynoglossus semilaevis). The results showed that both
recombinant LepA and LepB proteins of tongue sole
almost entirely suppressed the expression of genes
related to growth and reproduction in the
hypothalamic-pituitary-gonadal axis and liver, while
LepB may have had a positive role on steroid synthesis
in gonads. Further, a high concentration of LepA
facilitated the expression of IGF-{\Iota} in liver. At
the same time, we utilized human leptin to incubate
four tissues in vitro; although most of them had trends
similar to those stimulated with tongue sole leptins,
there were still some differences, indicating
differences among leptin homologs between humans and
fishes. To our knowledge, this is the first study to
explore the function of tongue sole LepA and LepB
within the hypothalamic-pituitary-gonadal axis and
liver in vitro. Our results provide a valuable resource
and foundation for future studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hasan:2023:CIM,
author = "Imam Hasan and Francesco Gai and Simona Cirrincione
and Simona Rimoldi and Giulio Saroglia and Genciana
Terova",
title = "Chitinase and Insect Meal in Aquaculture Nutrition: a
Comprehensive Overview of the Latest Achievements",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "607",
day = "14",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120607",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/607",
abstract = "The aquaculture industry is looking for sustainable
alternatives to conventional fish meals in fish feed,
and insect-based meals are proving to be a promising
solution. These meals are nutritionally optimal as they
have a high protein content and an ideal amino acid
profile. However, the presence of chitin, a component
of the insect exoskeleton in these meals presents both
an opportunity and a challenge. Chitosan, a derivative
of chitin, is known to improve the physiological
functions of fish, including growth, immunity, and
disease resistance. While chitin and its derivative
chitosan offer several physiological benefits, their
presence can affect the digestibility of feed in some
fish species, making the inclusion of insect-based
meals in aquafeeds complex. While studies suggest
positive effects, some problems, such as reduced growth
rates in certain species, emphasize the need for
further research on chitin digestion in fish.
Chitinase, an enzyme that breaks down chitin, is being
investigated as a potential solution to improve the
nutritional value of insect meals in aquafeed. This
review provides a comprehensive analysis of the
applications, benefits, and challenges of using
chitinase in aquaculture, highlighting the enzyme's
role in improving feed digestibility, disease control,
and environmental sustainability. Extensive research is
required to fully understand the potential of chitinase
enzymes in aquaculture and to optimize their
applications in this dynamic field. Overall, this
review provides insight into the evolving landscape of
insect-based meals and the applications of chitinase
enzymes within sustainable aquaculture practices.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shi:2023:EPT,
author = "Yongchuang Shi and Haibin Han and Fenghua Tang and
Shengmao Zhang and Wei Fan and Heng Zhang and Zuli Wu",
title = "Evaluation Performance of Three Standardization Models
to Estimate Catch-per-Unit-Effort: a Case Study on
\geoname{Pacific} Sardine (\bioname{Sardinops sagax})
in the \geoname{Northwest Pacific Ocean}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "606",
day = "11",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120606",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/606",
abstract = "Catch-per-unit-effort (CPUE) standardization in
fisheries is a critical foundation for conducting stock
assessment and fishery conservation. The Pacific
sardine (Sardinops sagax) is one of the economically
important fish species in the Northwest Pacific Ocean
(NPO). Hence, the importance of choosing an appropriate
CPUE standardization model cannot be overstated when it
comes to achieving a precise relative abundance index
for the efficient management of Pacific sardine
fishery. This study's main aim was to assess and
compare the efficacy of three models, specifically the
General Linear Model (GLM), the Generalized Linear
Mixed Model (GLMM), and the spatio-temporal GLMM
(VAST), in the CPUE standardization for Pacific sardine
fishery in the NPO, with the ultimate goal of
identifying the most appropriate model. An influence
analysis was applied to analyze the impact of
individual variables on the disparity among
standardized and nominal CPUE, and the main explanatory
variables influencing standardized CPUE were
identified. A coefficient--distribution--influence
(CDI) plot was generated to analyze the impact of the
different models on the annual standardized CPUE.
Additionally, a simulation testing framework was
developed to evaluate the estimated accuracy of the
three models. The results indicated that the
standardized CPUE and the nominal CPUE exhibited
similar trends between 2014 and 2021 for the three
models. Compared to the GLM and the GLMM, the VAST
demonstrates larger conditional R 2 and smaller
conditional AIC, indicating a better performance in
standardizing the CPUE for Pacific sardines due to its
consideration of spatial and temporal variations. The
interaction terms within the three models exert
significant influences on the annual standardized CPUE,
necessitating their inclusion in the model
construction. CDI plots indicate that the
spatio-temporal influence of the VAST model exhibits a
smaller variation trend, suggesting that the VAST is
more robust when standardizing the CPUE for Pacific
sardines. Simulation testing additionally demonstrated
that the VAST model displays smaller model root mean
squared error (RMSE) and bias, establishing it as the
superior performer for standardizing CPUE. Our results
provide a theoretical basis for the scientific
management of Pacific sardines in the NPO and can be
extended to CPUE standardization for other small
pelagic fish species worldwide.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nunes:2023:BSF,
author = "Alberto J. P. Nunes and Hiroshi Yamamoto and Jo{\~a}o
Paulo Sim{\~o}es and Jo{\~a}o Luiz Pisa and Nelson
Miyamoto and Jordana Sampaio Leite",
title = "The Black Soldier Fly (\bioname{Hermetia illucens})
Larvae Meal Can Cost-Effectively Replace Fish Meal in
Practical Nursery Diets for Post-Larval
\bioname{Penaeus vannamei} under High-Density Culture",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "605",
day = "10",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120605",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/605",
abstract = "The black soldier larvae meal (BSFLM) has been the
most extensively studied insect protein source in
shrimp nutrition. However, both the availability and
prices of BSFLM are still a constraint for its
widespread use as an ingredient in animal feeds. The
present study investigated the growth and economic
performance of post-larval (PL) P. vannamei fed nursery
diets with a progressive replacement of fish meal (FML)
for BSFLM at 0, 25, 50, 75, and 100\%. These
replacements corresponded to a dietary inclusion (\% of
the diet, as-is) of FML and BSFLM of 16.50 and 6.33\%,
11.00 and 13.04\%, 5.50 and 19.74\%, and 0 and 26.46\%,
respectively. A total of 102,647 shrimp at the age of
PL15 with 2.7 {\pm} 0.2 mg body weight (BW) were
stocked in fifty 1.5 m 3 tanks under 1369 PLs/m 3 (2053
{\pm} 33 PLs/tank) and reared for 42 days. Final shrimp
survival (90.5 {\pm} 7.6\%), daily weight gain (14.7
{\pm} 1.1 mg/day), and apparent feed intake (0.67 {\pm}
0.03 g of feed per stocked shrimp) were unaffected by
dietary treatment. The highest gained yield (791 {\pm}
52 and 776 {\pm} 38 g/m 3) and final BW (621 {\pm} 7.2
and 632 {\pm} 7.2 mg) were attained when FML was
replaced for BSFLM at 50 and 75\% with the lowest at
0\% (726 {\pm} 34 g/m 3 and 598 {\pm} 8.1 mg,
respectively). Shrimp fed diets with 0 and 100\%
replacement of FML exhibited the highest feed
conversion ratio (1.25 {\pm} 0.04 and 1.24 {\pm} 0.08)
compared to those fed a diet with 50\% (1.16 {\pm}
0.06). At a price of USD 2.00/kg, BSFLM demonstrated a
favorable ROI (return of investment) when compared to
FML, irrespective of the replacement level. With 25 and
50\% replacement, BSFLM remained cost-competitive up to
3.50 USD/kg. At 75\% FML replacement, there were no
significant differences in ROI with a price range of
2.00 up to 3.04 USD/kg. At full replacement, ROI
dropped significantly at a BSFLM price of 2.50 USD/kg
and beyond. It can be concluded that FML can be fully
replaced for BSFLM in well-balanced nursery diets for
P. vannamei. Although the full replacement of FML for
BSFLM was successfully accomplished, the competitive
ROI was sustained only when the price of BSFLM did not
exceed 3.04 USD/kg at its dietary highest inclusion of
19.74\%. Further research may be necessary to fine-tune
cost-effective inclusion levels of BSFLM to optimize
the economic outcomes while considering the fluctuating
prices of FML.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Shi:2023:EAG,
author = "Xuehui Shi and Xinyi Tang and Yichao Zhang and Wenqi
Wang and Siyong Qin and Qinghua Liu and Jie Mei",
title = "The Early Allometric Growth and Osteological Ontogeny
of Pot-Bellied Seahorse (\bioname{Hippocampus
abdominalis}, {L.} 1827) under Mass-Scale Captive
Breeding Conditions in \geoname{North China}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "604",
day = "08",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120604",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/604",
abstract = "Seahorses are valuable species for their use in
traditional Chinese medicine, as well as for the
aquarium trade as ornamentals and curiosities. To
balance market demand and reduce pressure on wild
populations, many countries have undertaken commercial
seahorse cultivation. Skeletal development plays a
crucial role in fish fry culture, affecting external
morphology, feeding, and movement. This study
investigated the ontogeny allometry, timing, and
progression of skeletal development in H. abdominalis
from DAB (day after birth) 1 to DAB 100 under
mass-scale captive breeding conditions in north China.
The results of this study revealed the growth rate was
significantly increased between DAB 30 and DAB 54.
Allometry analysis revealed that in the early stage,
the head, trunk, and tail demonstrated almost isometric
growth. However, in the later stage, the head and trunk
exhibited negative isometric growth, whereas the tail
displayed positive isometric growth. Skeletal staining
results showed that newborn seahorses do not have
ossified bones until DAB 11 (SL 28.14 {\pm} 2.94 mm).
Ossification was primarily observed in the jaw region
and the tubular nasal structure of the cranium, which
indicated the importance of the early development of
feeding organs. The initial formation of ossified
vertebral columns was observed at DAB 13 (SL 26.48
{\pm} 0.63 mm), with the complete ossification of all
vertebrae occurring by DAB 45 (SL 54.87 {\pm} 4.70 mm).
Furthermore, the cranium, rings, and plates were all
fully ossified by DAB 30. Ossification of the fins
began at DAB 23 (SL 31.27 {\pm} 4.05 mm). However,
neither of them were fully ossified by DAB 100. The
pelvic fin and the complete structure of the caudal fin
were not observed, possibly because of caudal fin ray
structure degeneration within the pouch. In addition,
no skeletal deformities were observed in all the tested
samples. The results of this study provide valuable
information on the developmental biology of H.
abdominalis, enriching our understanding of their
growth and offering insights for optimizing fish fry
breeding technologies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2023:EVF,
author = "Jiayang Li and Yu Guo and Xinye Zhao and Shengjie Zhou
and Zhenhua Ma and Gang Yu and Chuanxin Qin and
Xingqiang Wang",
title = "The Effects of Vibration Frequency on Oxidative
Stress, Digestive Enzymes and {ATPases} of Crimson
Snapper (\bioname{Lutjanus erythropterus}) Fry during
Transport",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "603",
day = "08",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120603",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/603",
abstract = "In this study, we sought to characterize the effect of
water vibration frequency stress on crimson snapper
(Lutjanus erythropterus) survival to determine an
optimal transportation speed. To achieve this, we used
a transport tank (25 cm $ \times $ 17 cm $ \times $ 16
cm) to simulate the transport process. After 8 h at
five different vibration frequencies (D1 = 75 rpm, D2 =
105 rpm, D3 = 135 rpm, D4 = 165 rpm, and D5 = 195 rpm),
the pH and dissolved oxygen (DO) levels in the tanks
decreased; ammonia nitrogen levels (NH 4 -N) and
temperature (T) increased with increasing density; and
significant changes in oxidative stress biomarkers,
digestive enzymes, and ATPase levels were observed in
crimson snapper fry. The enzyme activity increased and
reached the maximum value at 195 rpm. The experimental
results suggested that during the actual transport,
when using transport tanks, the length of the transport
time was less than 8 h, and setting the vibration
frequency for transportation at 135 rpm was more
appropriate, that is, a speed of 50 km/h for
transporting crimson snapper fry.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vaihola:2023:EFD,
author = "Siosaia Vaihola and Stuart Kininmonth",
title = "Environmental Factors Determine Tuna Fishing Vessels'
Behavior in \geoname{Tonga}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "602",
day = "07",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120602",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/602",
abstract = "Comprehending the spatial distribution of human
fishing endeavors holds significant importance in the
context of monitoring fishery resources and
implementing spatial management measures. To gain
insights into the spatial arrangement of tuna longline
activities within the exclusive economic zones of Tonga
and their correlation with the marine environment, this
study utilizes data from the Tonga Tuna Longline
Fisheries spanning from 2002 to 2018. The data are
employed to extract information about the spatial
distribution of fishing efforts and coupled with 15
marine environmental variables covering both sea
surface and subsurface conditions. This study employs
boosted regression trees (BRT) and general additive
models (GAM) to establish the non-linear relationships
between the distribution of fishing effort and marine
environmental factors. Furthermore, it examines and
analyzes the ecological niche occupied by tuna longline
vessels in high-sea environments. The outcomes of the
factor analysis indicate that the most important
factors influencing the fishing efforts of tuna
longliners are the dissolved oxygen content at the sea
surface and latitude. These two factors contribute
significantly, accounting for 19.06\% and 18.62\% of
the fishing efforts of vessels, respectively, followed
by distance to ports, longitude, and dissolved oxygen
at 100 m depth, contributing 10.77\%, 7.07\%, and
6.30\%, respectively. The sea surface chlorophyll,
ocean current at 100 m depth, and mixed layer depth
contributed the least, 3.63\%, 2.13\%, and 1.72,
respectively. In terms of space and time, tuna
longliners are more likely to operate in the
18--{22$^\circ $} S latitudinal and 172--{178$^\circ $}
W longitudinal region, and fishing efforts increased in
the months from March to August. The spatial
distribution of the fishing efforts modeled for fishing
vessels in 2018 is predicted to have good spatial
distribution with the actual fishing efforts of these
vessels. This research aids in comprehending the
environmental impacts resulting from shifts in the
spatial distribution of tuna longline vessels, offering
valuable insights for the effective management of tuna
longline fisheries in Tonga.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:CXA,
author = "Qian Xu and Zheng Yang and Siyu Chen and Wenjuan Zhu
and Siyuan Xiao and Jing Liu and Hongquan Wang and
Shile Lan",
title = "Correction: {Xu et al. Effects of Replacing Dietary
Fish Meal by Soybean Meal Co-Fermented Using
\bioname{Bacillus subtilis} and \bioname{Enterococcus
faecium} on Serum Antioxidant Indices and Gut
Microbiota of Crucian Carp \bioname{Carassius auratus}.
{{\booktitle{Fishes}}} 2022, {\bf 7}, 54}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "601",
day = "07",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120601",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Xu:2022:ERD}.",
URL = "https://www.mdpi.com/2410-3888/8/12/601",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cohen-Sanchez:2023:IOS,
author = "Amanda Cohen-S{\'a}nchez and Antoni Gabriel
S{\'a}nchez-Mairata and Jos{\'e} Mar{\'\i}a Valencia
and Antonio Box and Samuel Pinya and Silvia Tejada and
Antoni Sureda",
title = "Immune and Oxidative Stress Response of the Fish
\bioname{Xyrichthys novacula} Infected with the
Trematode \bioname{Ectoparasite scaphanocephalus} sp.
in the \geoname{Balearic Islands}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "600",
day = "06",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120600",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/600",
abstract = "Global change produces substantial modification to the
distribution and rhythm of infection of diseases in
fish, favouring the introduction of new pathogens.
Recently, the presence of black spot disease,
associated with a digenean fluke of the genus
Scaphanocephalus, has been observed in specimens of
Xyrichthys novacula on the island of Ibiza (Balearic
Islands). The aim of the present study was to evaluate
the antioxidant and immune response in both the skin
mucus and spleen of X. novacula depending on the degree
of infection by Scaphanocephalus sp. The specimens were
captured in a control area, without the presence of the
parasite, and in an affected area, classifying the fish
as low infection (1--15 spots) and high infection
({$>$15} spots). As the degree of infection increased,
a decrease in the body condition index was observed.
The activity of the antioxidant enzymes in the
mucus---catalase, superoxide dismutase and glutathione
peroxidase---increased progressively with the degree of
infection. This activation of antioxidant defences was
not enough to prevent an increase in malondialdehyde
levels, an indicator of oxidative damage, in the group
with the highest infection. Similarly, an increase in
immunological parameters---lysozyme, alkaline
phosphatase, myeloperoxidase and immunoglobulins---was
observed in mucus as infection increased. Regarding the
spleen, only an increase in lysozyme activity and
alkaline phosphatase in fish with a greater severity of
infection was observed. In conclusion, as the severity
of Scaphanocephalus sp. infection increased, it induced
an immune and oxidative stress response in skin mucus,
leading to a decrease in overall body condition. The
potential health effects that the ectoparasite may have
on X. novacula populations will require follow-up
studies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Johnston:2023:SDB,
author = "Samuel J. Johnston and Jason B. Smith and Brady D.
Slater and Jonathan P. Doubek",
title = "Spatial Density, Biomass, and Composition of
Crustacean Zooplankton on \geoname{Lake Michigan}
Beaches",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "599",
day = "06",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120599",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/599",
abstract = "Ecosystem alterations to Lake Michigan (LM), such as
the invasion of dreissenid mussels, have decreased the
abundance of phytoplankton and, subsequently,
zooplankton, which has implications for Great Lake
fisheries. The community composition of zooplankton has
also been altered over the past 20 years in LM,
shifting the summer dominance from cladoceran to
calanoid taxa. However, most of our information on
zooplankton community dynamics is from deeper pelagic
zones. The nearshore beach ({\leq}1 m) habitat, which
serves as a critical nursery for some larvae and
juvenile fish, is composed of different zooplankton
taxa than deeper zones, but limited data are available
for comparison. We conducted a standardized summer
sampling campaign to characterize the zooplankton
community across 32 beaches in LM. We found the lowest
crustacean zooplankton density and biomass on northern
LM beaches and a higher zooplankton density and biomass
on Greater Green Bay and central LM beaches, which had
warmer water temperatures. Smaller-bodied cladocera
(mainly Bosmina) and cyclopoid taxa were the most
abundant groups on beaches. Our results provide the
first comprehensive characterization of zooplankton on
beaches in the Great Lakes, with applications for other
lake systems. These results may help identify potential
``hot spots'' of fish recruitment for management.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2023:EUP,
author = "Yingming Yang and Wenlong Li and Run Wang and Dan Xu
and Yadong Chen and Zhongkai Cui and Songlin Chen",
title = "Effects of \bioname{Ulva prolifera} Degradation on
Growth Performance and Antioxidant Capacity of
\geoname{Japanese} Flounder (\bioname{Paralichthys
olivaceus}) Family",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "598",
day = "05",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120598",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/598",
abstract = "Massive macroalgae blooms, primarily caused by the
overgrowth of Ulva prolifera (U. prolifera) in the
Yellow Sea of China, pose a severe risk to both marine
organisms and the aquaculture industry. This study's
aim was to evaluate the impact of U. prolifera
degradation on the growth performance and antioxidant
capacity of Japanese flounder (Paralichthys olivaceus)
and select some potential Japanese flounder families
(labeled 2101--2108, established by crossbreeding)
tolerating U. prolifera degradation conditions. Thus, a
60-day U. prolifera exposure experiment was conducted.
The results showed that the contents of Na, K, Mg, and
Fe elements in the U. prolifera degradation water were
significantly increased. The specific growth rate and
survival rate of flounder were significantly decreased
under the U. prolifera degradation condition, while the
2101 and 2103 flounder families showed a better growth
performance compared with the positive control (PC)
group. Moreover, the results showed that activities of
total antioxidant capacity (T-AOC), transaminases, and
alkaline phosphatase (AKP) in serum were significantly
decreased, while the 2103 flounder family showed higher
activities. Furthermore, U. prolifera degradation
significantly increased superoxide dismutase (SOD)
activity and glutathione (GSH) content while decreasing
catalase (CAT) activity and malondialdehyde (MDA)
content in the liver. Specifically, SOD and CAT
activities of the 2103 flounder family were higher than
the 2101 flounder family and PC group. In addition, the
gill SOD and CAT activities of the 2103 flounder family
were significantly higher than the PC group. Similarly,
the antioxidant-related gene (sod and cat) expressions
were synchronously upregulated or downregulated in the
liver and gills in response to U. prolifera
degradation. These results revealed that U. prolifera
degradation decreased the growth performance and
influenced the antioxidant capacity of Japanese
flounder, while the 2103 flounder family had better
advantages in the U. prolifera degradation condition.
Therefore, the 2103 flounder family could be regarded
as the potential flounder family tolerating U.
prolifera degradation. The increased Fe content in the
U. prolifera degradation water may be one of the main
causes of the physiological alterations observed in
Japanese flounder.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2023:ICS,
author = "Zhongyuan Chen and Ronghua Wang and Ruisong Wang and
Naitong Yu and Pinhong Yang",
title = "Identification and Characterization of the Stimulator
of Interferon Genes ({STING}) in \geoname{Chinese}
Giant Salamander \bioname{Andrias davidianus}",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "597",
day = "04",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120597",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/597",
abstract = "The stimulator of interferon genes (STING) is a
central and multifaceted mediator in innate immunity,
and plays vital roles in defending against pathogen
infection. In this study, we identified and
functionally characterized the STING homolog from the
Chinese giant salamander Andrias davidianus (AdSTING)
for the first time. The open reading frame of AdSTING
encodes a 362 amino acid protein with a predicted
molecular mass of 41.6 kDa, which shares 31.1--46.7\%
of its sequence identity with STING homologs in other
vertebrates. Structural analysis revealed that AdSTING
possesses four predicted transmembrane domains (TMs) at
the N-terminal, and a C-terminal domain (CTD) featuring
a dimerization domain (DD), a c-di-GMP-binding domain
(CBD), and a short C-terminal tail (CTT). Tissue
distribution analysis showed that AdSTING mRNA was
ubiquitously expressed in all examined tissues, with
abundant expression in muscles, intestine, and thymus.
During Andrias davidianus ranavirus (ADRV) infection,
significant up-regulation of AdSTING expression was
observed in the thymus, spleen, and kidney. Upon
different stimuli in vitro, the expression of AdSTING
was significantly induced by ADRV infection or
polyinosin-polycytidylic acid (poly I:C) stimulation,
but no obvious changes were observed during
lipopolysaccharide (LPS) stimulation. Subcellular
localization analysis revealed that AdSTING mainly
localized in the cytoplasm in the Chinese giant
salamander thymus cell line (GSTC) and co-localized
with the endoplasmic reticulum (ER). Luciferase
reporter assays confirmed the ability of AdSTING to
activate the interferon-stimulated response element
(ISRE) and interferon (IFN) promoter. Furthermore,
overexpression of AdSTING effectively decreased ADRV
infection, as evidenced by the reduction of virus
titers and viral gene expression. Collectively, our
findings underscore the pivotal role of AdSTING in the
antiviral innate immunity of the Chinese giant
salamander, offering insights into the functional
evolution of STING in amphibians.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Schakmann:2023:IBS,
author = "Mathias Schakmann and Emil Aputsiaq Flindt Christensen
and John Fleng Steffensen and Morten Bo S{\o}ndergaard
Svendsen",
title = "The Influence of Body Size on Behavioral Thermal
Preference in \geoname{Atlantic} Cod (\bioname{Gadus
morhua}): Larger Fish Favor Colder Waters",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "596",
day = "02",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120596",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/596",
abstract = "In the context of global warming and the concurrent
decrease in ectothermic fish body size, the mechanisms
driving this phenomenon remain a subject of scientific
debate. This study, utilizing the Atlantic cod (Gadus
morhua) as a model organism, delves into the behavioral
size-dependent temperature preference in the context of
climate change. A significant negative correlation
between temperature preference and fish size was
discovered, aligning with in situ habitat temperatures
and optimal physiological performance metrics from
other studies. This correlation suggests that larger
fish exhibit a behavioral preference for colder areas,
potentially leading to shifts in distribution toward
polar regions or deeper waters in response to local
global warming. The findings contribute to predictions
of species distribution shifts, emphasizing the
critical role of size-dependent temperature preference
in shaping fish populations and offering valuable
insights for conservation efforts. Additionally, the
study uncovers a noteworthy relationship between body
size and thermal safety margins in fish behavior,
providing a novel avenue for future research into the
intricate dynamics of thermal regulation in response to
climate change. Overall, this research enhances our
understanding of the complex interplay between
temperature, fish size, and ecological responses,
offering crucial information for informed conservation
and management strategies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2023:ESP,
author = "Wenqian Wang and Shengyan Su and Ping Dong and Wenrong
Feng and Jianlin Li and Chengfeng Zhang and Yongkai
Tang",
title = "Effects of Seasonal Photoperiod on Growth, Lipid
Metabolism, and Antioxidant Response in the
\geoname{Huanghe} Carp (\bioname{Cyprinus carpio
haematopterus})",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "595",
day = "01",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120595",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/595",
abstract = "Photoperiod is one of the most important environmental
cues for organisms, and it plays a crucial role in
regulating feeding, behavior, growth, and metabolism.
However, seasonal photoperiods are often overlooked in
carp culture or experiments, with a poorly understood
effect on lipid metabolism and oxidative stress in
fish. To explore the effects of seasonal photoperiods,
we exposed Huanghe carp (Cyprinus carpio haematopterus)
to summer photoperiod (14 h light:10 h dark) and winter
photoperiod (10 h light:14 h dark) daylight conditions
in an eight-week experiment. Our results suggested that
the winter photoperiod significantly increased the
liver TG level as well as the transcript levels of
genes related to lipid synthesis, indicating that the
lipid metabolism in Huanghe carp liver was enhanced
compared to summer photoperiod conditions, and that
lipid deposition may be responsible for the increase in
body weight level and hepatosomatic index.
Additionally, MDA, GSH, GSH-PX, and T-AOC levels were
significantly elevated in the liver of fish under the
winter photoperiod, suggesting that Huanghe carp
responded to winter photoperiod exposure-induced
oxidative stress in the liver by enhancing the
antioxidant response. Based on transcriptome analysis,
the winter photoperiod activated hepatic autophagy
response and the FOXO signaling pathway in Huanghe
carp. Combined with the correlation analysis, the
Huanghe carp maintains the physiological health of the
liver by activating the FOXO signaling pathway-mediated
cell cycle regulation and autophagy response in
response to oxidative stress during winter photoperiod
exposure. Our study provides the first evidence for the
physiological regulation of the liver in Huanghe carp
under seasonal photoperiod stimulation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2023:EAM,
author = "Jing Xu and Gangfu Chen and Min Wu and Qihui Yang and
Huatao Li",
title = "The Extract of \bioname{Astragalus membranaceus}
Inhibits Lipid Oxidation in Fish Feed and Enhances
Growth Performance and Antioxidant Capacity in {Jian}
Carp (\bioname{Cyprinus carpio} var. {Jian})",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "594",
day = "01",
month = dec,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120594",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/594",
abstract = "In this study, a linoleic and linolenic acid were
incubated with petroleum ether extract, ethyl acetate
extract, acetone extract (AE) and aqueous extract of
Astragalus membranaceus. The phenolic content and total
antioxidant capacity (T-AOC) were determined in the
extracts of Astragalus membranaceus (EAms) above.
Results showed that EAms decreased the levels of
malonaldehyde, conjugated diene, and peroxide value
levels in material above. Of all of EAms, AE showed the
strongest T-AOC and inhibitory effect on the lipid
oxidation. Next, fish feeds were incubated with graded
levels of AE. The results showed that AE inhibited
lipid oxidation in fish feed. The appropriate dosage
for reducing lipid oxidation was 6.74 g AE kg {$-$1}
feeds. The effect of EAms on the lipid oxidation may be
closely associated with their phenolic content. Then,
juvenile Jian carp (Cyprinus carpio var. Jian, 10.2
{\pm} 0.3 g) were fed with diets containing graded
levels of AE (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and
7.0 g kg {$-$1}) for 60 days. Current data displayed
that dietary AE increased the growth performance of
fish. The optimum dosage for growth promotion was 5.15
g AE kg {$-$1} diet. This result of AE may be ascribed
to its enhancing effect on the activity of digestive
and absorptive enzymes and antioxidant capacity in
digestive organs of fish. Our present study indicated
that EAm holds promise as a natural antioxidant for
fish and their feed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cao:2023:BTA,
author = "Zhichen Cao and Denghua Yin and Zhanwei Li and Yan Yan
and Peng Zhang and Sigang Zhang and Danqing Lin and
Zhong Hua and Jialu Zhang and Congping Ying and Han
Zhang and Pao Xu and Guixin Dong and Kai Liu",
title = "Blood Transcriptome Analysis Provides Responsive
Changes in Gene Expression between \latinname{Ex Situ}
and Captive \geoname{Yangtze} Finless Porpoises
(\bioname{Neophocaena asiaeorientalis
asiaeorientalis})",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "593",
day = "30",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120593",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/593",
abstract = "The Yangtze finless porpoise (Neophocaena
asiaeorientalis asiaeorientalis, YFP) is an endangered
species endemic to the Yangtze River in China, and it
is the only freshwater whale in the genus Neophocaena.
In terms of protection, three effective conservation
strategies exist: in situ conservation, ex situ
conservation, and artificial breeding, all of which
have been implemented by the Chinese government. Of
these, ex situ conservation involves the relocation of
Yangtze finless porpoises to semi-natural waters with
less human interference, and artificial breeding
involves the relocation of Yangtze finless porpoises to
a controlled environment that is more strictly managed.
To compare and analyze the responsive changes in gene
expression of the YFPs between the ex situ and
controlled environments, we performed the RNA
sequencing of blood tissues from these YFPs. A total of
1201 differentially expressed genes (DEGs) were
identified, of which 423 were up-regulated in the ex
situ population and 778 were up-regulated in the
controlled-environment population. Gene enrichment
analysis showed that 1201 DEGs between the ex situ and
controlled-environment populations were generally
enriched for vision-, digestion- and
immune-system-related pathways. Further analysis
revealed that several key immune system pathways, such
as the chemokine signaling pathway and B cell receptor
signal pathway, were activated in the ex situ
population. In addition, the key pathways related to
vision, including phototransduction and the
inflammatory mediator regulation of TRP channels, as
well as the pathways related to the digestive system,
such as protein digestion and absorption and salivary
secretion, were activated in the controlled-environment
population. These results suggest that the ex situ
populations may respond to complex environmental
conditions in semi-natural waters by enhancing their
immune function through the increased expression of
immune-related genes and that the visual function and
protein digestion of the YFPs were improved compared to
those of the ex situ population based on the conditions
of artificial feeding, such as the higher transparency
of the water and regular feeding. This study provides
clues for evaluating the adaptability of YFPs to
different environments and is a useful reference for
future ex situ conservation and artificial breeding.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{David-Ruales:2023:ZPE,
author = "Carlos David-Ruales and D{\'e}bora Machado Fracalossi
and Felipe Collazos-Lasso",
title = "Zootechnical Parameters and Enzyme Activity in the
Species \bioname{Brycon moorei} ({Steindachner} 1878)",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "592",
day = "30",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120592",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/592",
abstract = "Brycon moorei is an opportunistic omnivorous species.
It is not known what its nutritional requirements are
at any stage of its development; this study determined
the influence of diet on the zootechnical parameters
and enzyme activity of these fish. In a completely
randomized quintuplicate design, an ad libitum dietary
protocol was applied, which included Prochilodus
magdalenae larvae, Artemia salina and balanced feed
with increasing inclusion levels (35 to 55\% crude
protein (CP)). Larvae 22 h post-hatching (HPH), with an
average initial weight and length of 3.07 {\pm} 0.69 mg
and 6.069 {\pm} 0.22 mm, respectively, were seeded at a
density of 17 larvae L {$-$1} in tanks with an
effective volume of 60 L. The main zootechnical
parameters, water quality and enzyme activity were
analyzed with respect to the experimental diets.
Significantly, the best diet was that of 45\% CP when
compared with the other CP levels used. Specific serum
enzyme activity was found from hatching, with
fluctuating values, the specific pepsin-like activity
started at 111 HPH. B. moorei larvae can receive a dry
diet from 124 HPH and their requirement corresponds to
48.5\% CP.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2023:FRU,
author = "Liang Liu and Junfeng Wu and Tao Zheng and Haiyan Zhao
and Han Kong and Boyu Qu and Hong Yu",
title = "Fish Recognition in the Underwater Environment Using
an Improved {ArcFace} Loss for Precision Aquaculture",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "591",
day = "30",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120591",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/591",
abstract = "Accurate fish individual recognition is one of the
critical technologies for large-scale fishery farming
when trying to achieve accurate, green farming and
sustainable development. It is an essential link for
aquaculture to move toward automation and intelligence.
However, existing fish individual data collection
methods cannot cope with the interference of light,
blur, and pose in the natural underwater environment,
which makes the captured fish individual images of poor
quality. These low-quality images can cause significant
interference with the training of recognition networks.
In order to solve the above problems, this paper
proposes an underwater fish individual recognition
method (FishFace) that combines data quality assessment
and loss weighting. First, we introduce the Gem pooing
and quality evaluation module, which is based on
EfficientNet. This module is an improved fish
recognition network that can evaluate the quality of
fish images well, and it does not need additional
labels; second, we propose a new loss function,
FishFace Loss, which will weigh the loss according to
the quality of the image so that the model focuses more
on recognizable fish images, and less on images that
are difficult to recognize. Finally, we collect a
dataset for fish individual recognition (WideFish),
which contains and annotates 5000 images of 300 fish.
The experimental results show that, compared with the
state-of-the-art individual recognition methods, Rank1
accuracy is improved by 2.60\% and 3.12\% on the public
dataset DlouFish and the proposed WideFish dataset,
respectively.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barrows:2023:IKM,
author = "Frederick T. Barrows and Kelly B. Campbell and T.
Gibson Gaylord and Rodrigo C. M. Sanchez and Sergio A.
Castillo and Ewen McLean",
title = "Influence of Krill Meal on the Performance of
Post-Smolt \geoname{Atlantic} Salmon That Are Fed
Plant-Based and Animal-Based Fishmeal and Fish Oil-Free
Diets",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "590",
day = "30",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120590",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/590",
abstract = "The purpose of this study was to determine the
influence of krill meal (KM) inclusion at various
levels (0\%, 2.5\%, 5\%) in plant-based and
animal-based feeds, that were fishmeal (FM) and fish
oil (FO) free, on Atlantic salmon growth. A FM/FO feed
containing 0\% KM was the control. Using a 2 $ \times $
3 factorial approach, diets were randomly assigned to
one of 28 0.5 m 3 flow-through tanks (n = 4 tanks per
diet) initially stocked with 60 fish (148.4 {\pm} 12.9
g; 23.6 {\pm} 0.8 cm; condition factor (K) = 1.16 {\pm}
0.08) each. Salmon were fed for 90 days using automatic
feeders ad libitum. On day 45, stocking densities were
reduced to 45 fish per tank by the random removal of 15
individuals to remove any potential of density
affecting growth through the trial end. Water
temperature, oxygen saturation, pH, and salinity
throughout the trial were 11.8 {$^\circ $}C, 103.5\%,
7.38, and 32.0 g L {$-$1}, respectively. Fish fed
plant-based feed without KM were lighter (p {$<$} 0.05)
than all other groups at day 45 and 90, but those fed a
plant-based feed with KM had comparable growth and feed
intake compared to that of fish fed the control diet.
Irrespective of the presence of KM, animal-based feeds
achieved comparable weight growth (p {$>$} 0.05) to the
control and 5\% KM plant-based groups, with KM
increasing feed intake (p {$<$} 0.05). Between day 45
and 90, feed conversion ratios increased in all groups
except the control and 0\% KM plant-based group, while
specific growth rates (SGRs) decreased for all except
the 0\% KM plant-based diet. Between-group differences
(p {$<$} 0.05) were also noted for the thermal growth
coefficient. No differences were recorded in visceral
or intestinal weight, and whole-body lipid levels were
identical, proportional for all groups. Although
differences (p {$<$} 0.05) were apparent in the
concentrations of individual fillet fatty acids between
groups, a 75 g serving size of any treatment would be
sufficient to exceed daily intake recommendations for
EPA + DHA. This trial determined that benefit, in terms
of feed intake and growth performance, was gained when
KM was added to plant-based feeds. However, no such
advantage was observed when KM was used with
animal-based feeds.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rajalakshmi:2023:CED,
author = "Kaakarlu Shivakumar Vinanthi Rajalakshmi and Wen-Chao
Liu and Balasubramanian Balamuralikrishnan and Arun
Meyyazhagan and Govindharajan Sattanathan and
Manikantan Pappuswamy and Kadanthottu Sebastian Joseph
and Kuppusamy Alagesan Paari and Jang-Won Lee",
title = "Cadmium as an Endocrine Disruptor That Hinders the
Reproductive and Developmental Pathways in Freshwater
Fish: a Review",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "589",
day = "30",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120589",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/589",
abstract = "Cadmium (Cd) is a non-essential element with
sub-lethal effects even at low concentrations. The
persistent nature of Cd and its tendency to
bioaccumulate eventually create harmful effects on
water biota, including fish. Cd affects various aspects
of hormonal action in fish since it bioaccumulates in
the endocrine system and hinders the synthesis,
secretion, and metabolic activity of hormones, causing
severe damage along the
hypothalamus--pituitary--gonadal axis. Linking
reproductive and developmental impairments in fish with
ecologically relevant concentrations of individual
metals can be challenging due to the complexity of
aquatic ecosystems. This review deliberated the
significant and novel trends of toxicological
difficulties and approaches, including elucidating
environmental sources' bioavailability and Cd-induced
toxic effects in freshwater fish. Both acute and
chronic exposure to Cd can cause a range of adverse
effects, such as growth inhibition, impaired
reproductive capacity, endocrine disruption, and
developmental abnormalities in freshwater fish, as
evidenced by the present review. These investigations
support the concept of Cd as a naturally available
pollutant that causes irreversible damage in fish.
These findings will help to understand the etiology of
environmental circumstances that pose substantial
dangers to fish health and are also crucial for
preventing and treating exposure-related reproductive
disturbances in freshwater fish due to environmental
pollution.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cruces:2023:PBG,
author = "Celso Luis Cruces and Raquel Sim{\~o}es and Arnaldo
Maldonado J{\'u}nior and Ruperto Severino and Jhon
Darly Chero and Jos{\'e} Luis Luque",
title = "Proposal of \bioname{Brotulella} n. gen. for
Monogeneans from the Gills of the \geoname{Pacific}
Bearded Brotula \bioname{Brotula clarkae} {Hubbs}, 1944
({Ophidiiformes: Ophidiidae}) Based on Morphological
and Molecular Evidence",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "588",
day = "30",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120588",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/588",
abstract = "Based on morphological and molecular data, Brotulella
n. gen. is proposed to accommodate the dactylogyrid
monogeneans Brotulella laurafernandae n. sp. (type
species) and Brotulella luisahelenae n. sp. on the gill
filaments of the Pacific bearded brotula Brotula
clarkae Hubbs, 1944 (Ophidiiformes: Ophidiidae) from
Puerto Pizarro in the Tumbes region (northern Peru).
Species of the new genus are distinguished from all
other dactylogyrids by the combination of the following
features: (1) anchors with a stocking-shaped sheath
associated with the distal end of superficial and deep
roots, (2) tandem gonads, (3) a vas deferens looping
left intestinal caecum, (4) a distally twisted male
copulatory organ (MCO) with a delicate membranous
accessory piece articulated to the shaft of the MCO,
(5) a U-shaped ovary, (6) an almost sigmoid seminal
vesicle, and (7) two prostatic reservoirs with thick
muscular walls. A 28S ribosomal DNA-based phylogenetic
analysis (Maximum likelihood and Bayesian inference) of
sequences of two new species of Brotulella n. gen. from
the Southeastern Pacific Ocean, along with sequences
from closely related genera of the marine
Dactylogyridae, supports the establishment of the new
genus for the dactylogyrid parasites on the gills of
the Pacific bearded brotula.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2023:TTE,
author = "Zhibin Zhou and Lubo Wang and Mingqin Dai and Qingyan
Gao and Peng Wang and Lili Zhao and Yanlu Li and Rui Xi
and Mingchao Pan and Qiang Ma and Houguo Xu and
Mengqing Liang and Yuliang Wei",
title = "Three Types of \bioname{Enteromorpha prolifera}
Bio-Products Based on Different Processing Procedures
as Feed Additives in the Diets of \geoname{Pacific}
White Shrimp (\bioname{Litopenaeus vannamei})",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "587",
day = "29",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120587",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/587",
abstract = "A feeding trial was conducted to evaluate the
biological activities of Enteromorpha prolifera
bio-products in the diets of Pacific white shrimp
(Litopenaeus vannamei). Bio-products of E. prolifera
included E. prolifera meal, E. prolifera hydrolysate
and E. prolifera polysaccharide, which was supplied
using different processing procedures. The control diet
was supplemented without any E. prolifera bio-products
or dietary attractants. Experimental diets were
formulated to contain 0.2\% and 0.4\% of E. prolifera
hydrolysate (EPH0.2\% and EPH0.4\%), 0.03\% of E.
prolifera polysaccharide (EPP0.03\%), 3\% of E.
prolifera meal (EPM3\%), 0.1\% of dimethyl- {\beta}
-propiothetn (DMPT0.1\%) and 1\% of squid paste
(SP1\%). Shrimp ($ \approx $8 g) were randomly
distributed in 21 tanks and fed for 44 days. Feed
intake showed that 3\% of E. prolifera meal and 0.4\%
of E. prolifera hydrolysate in diets exhibited similar
attractant effects as 0.1\% of DMPT. Gross qualitative
observation showed that the abundance of lipid droplets
decreased in hepatopancreas of the EPH0.4\% and EPM3\%
groups, which were supported by hepatopancreas
triacylglycerol (TG), where significantly lower
concentrations were observed in the EPH0.4\% and EPM3\%
groups compared with the control group. Similarly, TG
and low-density lipoprotein cholesterol in serum
significantly decreased in the EPH0.4\%, EPP0.03\% and
EPM3\% groups compared to the control group.
Supplemental E. prolifera bio-products resulted in
significantly higher serum glutathione level of
EPP0.03\% or superoxide dismutase activities of
EPH0.4\% and EPM3\%, but significantly lower serum
malondialdehyde level of EPM3\%. In addition, tnf -
{\alpha} expression in hepatopancreas was significantly
down-regulated in shrimp fed the EPH0.2\%, EPH0.4\% and
EPM3\% diets. Based on survival analysis, E. prolifera
bio-products improved the resistance of shrimp to
hypoxic stresses. Thus, this study confirmed that
bio-products of E. prolifera supplied using different
processing procedures could be used to reduce the
amount of E. prolifera in feed of shrimp without
compromising their biological functions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Costa:2023:BRO,
author = "L{\'e}a Carolina de Oliveira Costa and Andrezza
Carvalho and Mariana Holanda and Jorge Santos and
Luc{\'e}lia Borges and Bruna Guterres and Je Nam Junior
and Virginia Fonseca and Larissa Muller and Luis Romano
and Silvia Botelho and Marcelo Pias and Juliane Ventura
and Lu{\'\i}s H. Poersch",
title = "Biological Responses of Oyster \bioname{Crassostrea
gasar} Exposed to Different Concentrations of Biofloc",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "586",
day = "29",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120586",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/586",
abstract = "Oysters have the potential to be a part of more
sustainable farming systems, such as multitrophic
systems integrated into biofloc systems, due to their
filtration activity, which enables them to act as
organic consumers. However, the stress experienced by
animals in a system with a high organic load can
compromise their productive performance. The objective
of this study was to evaluate the biological responses
of Crassostrea gasar oysters when exposed to different
concentrations of total suspended solids in biofloc
systems. The oysters were exposed to four different
concentrations of solids for 28 days. Hall effect
sensors were installed on the outside of the shells to
detect the movement of the oyster valves. Also,
biochemical and histological analyses were conducted to
assess the biological responses of the oysters to
exposure to varying levels of solids. A difference in
valve opening detected by the Hall sensors was observed
from the second week of culture, indicating a
relationship between shell closure and higher
concentrations of suspended solids present in the
system. In terms of biochemical analysis, a significant
increase in lipid damage was observed in treatments
with medium and high levels of total suspended solids
compared with the control group. Conversely, no changes
were observed in the gill structure of the oysters
caused by the concentrations of suspended solids in the
system when compared with the control. According to the
analyses of gill activity and biochemistry, it is
suggested that C. gasar should be cultured with total
suspended solids at less than 200 mg/L. Oysters
cultivated in a biofloc system keep their shells closed
when subjected to high concentrations of total
suspended solids; concentrations of total suspended
solids below 200 mg/L do not induce oxidative stress,
changes in behavior or histological alterations in C.
gasar oysters cultivated in a biofloc system.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kamikawa:2023:GCR,
author = "Keith Kamikawa and Brian W. Bowen and Donald Kobayashi
and Kimberly Peyton and Elizabeth Wallace",
title = "Genetic Connectivity of Roundjaw Bonefish
\bioname{Albula glossodonta} ({Elopomorpha, Albulidae})
in the \geoname{Central Pacific Ocean} Resolved through
{ddRAD}-Based Population Genomics",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "585",
day = "29",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120585",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/585",
abstract = "Bonefishes are a nearshore species targeted by
non-commercial anglers and subsistence fishers in the
Central and South Pacific islands. Among the bonefish
species in the Indo-Pacific region, Albula glossodonta
are known to have one of the widest geographic ranges,
from the Red Sea to the Central Pacific, but it is
unknown how dispersive A. glossodonta are between
geographically isolated islands. Volunteer anglers
collected A. glossodonta fin clips from the main
Hawaiian Islands in the North Pacific, Anaa Atoll in
the South Pacific, and intermediate Kiritimati Island
(Line Islands) to assess the scale of dispersal and
population structure within the Pacific Ocean.
Population genomics was conducted based on 208
individuals and 7225 SNPs. Although adult A.
glossodonta exhibit strong site fidelity, genomic
results show no population differentiation between Oahu
and Maui in Hawai`i. Bonefishes exhibit significant
population structure between Anaa and Hawai`i (F ST =
0.096), with intermediate Kiritimati comprising admixed
fishes. A lengthy larval duration likely promotes
connectivity between Pacific islands. Regional
management regimes may be most appropriate for a
species with this level of dispersal.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Iitembu:2023:CDA,
author = "Johannes A. Iitembu and Daniel Fitzgerald and
Themistoklis Altintzoglou and Pierre Boudry and Peter
Britz and Carrie J. Byron and Daniel Delago and Sophie
Girard and Colin Hannon and Marcia Kafensztok and
Francisco Lagreze and Jefferson Francisco Alves Legat
and Angela Puchnick Legat and Adriane K. Michaelis and
Ingelinn Eskildsen Pleym and Simone S{\"u}hnel and
William Walton and {\AA}sa Strand",
title = "Comparative Description and Analysis of Oyster
Aquaculture in Selected \geoname{Atlantic} Regions:
Production, Market Dynamics, and Consumption Patterns",
journal = j-FISHES,
volume = "8",
number = "12",
pages = "584",
day = "29",
month = nov,
year = "2023",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes8120584",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:15 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/8/12/584",
abstract = "In the face of an increasing world population and a
subsequent need for an increase in sustainable and
healthy food production, low trophic species, such as
oysters, emerge as a promising alternative. However,
regional variations in oyster production techniques,
market dynamics, and consumption patterns create
challenges for both the global and local industry's
growth. In this study, a descriptive qualitative
analysis of oyster markets across seven Atlantic
regions was carried out. The Pacific oyster
(Crassostrea gigas) was found to be farmed in most
Atlantic regions except the US but is classified as
invasive in Sweden and potentially invasive in South
Africa. Other farmed and/or harvested species include
native species (C. gasar and C. rhizophorae) in Brazil,
the American cupped oyster (C. virginica) in the US,
and the European flat oyster (Ostrea edulis) in France,
Sweden, and the US. In Irish farms, Pacific oysters are
primarily for export to European markets. The marine
aquaculture sectors of Sweden, South Africa, and
Namibia, as well as Brazil's farming for C. gasar, were
found to be underdeveloped. This study also observed a
variation in licensing, property rights, and regulatory
frameworks. Financial challenges for small businesses,
ecological implications of seed production techniques,
biosecurity risks, and public health considerations are
emphasized as critical areas for attention. This study
offers valuable insights into the selected markets and
can serve as a useful resource for policymakers,
aquaculture practitioners, and stakeholders in
optimizing global shellfish industry strategies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sayed:2024:ICP,
author = "Ramy K. A. Sayed and Doaa M. Mokhtar and Madeha Ahmed
Hashim and Ahmed S. Aly and Giacomo Zaccone and Marco
Albano and Alessio Alesci and Nada Abdellah",
title = "Immune Cell Profiling in the Ovarian Stroma of a
Viviparous Fish during the Breeding Season: a
Histological and Immunohistochemical Investigation",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "10",
day = "25",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010010",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/10",
abstract = "The molly fish is a member of viviparous teleosts that
are characterized by the fusion of the right and left
ovaries during their early embryonic development. This
fusion results in a singular and saccular ovary, where
the germinal epithelium lines the internal lumen. The
present study aimed to identify the immune cells in the
ovarian stroma of Molly fish during the breeding season
using histological and immunohistochemical analysis.
Histological examination of the ovaries displayed
oocytes at all different stages of development and
degeneration. The ovocoel, a lymph-filled space,
remains in the center of the ovary and branches
posteriorly, creating the lumen of the gonoduct. The
ovarian wall is composed of three layers: the
mesothelium, tunica albuginea, and germinal epithelium.
The developing ova were held together by the stroma,
which consisted of vascular collagenous connective
tissue clustered with immune cells. Immunohistochemical
analysis revealed the presence of clusters of
macrophages expressing APG5, IL-1{\beta}, TGF-{\beta},
S100, NF-{\kappa}B, CD68, Iba-1, and Ach. Monocytes
demonstrated positive immunoreactivity for both APG5
and IL-1{\beta}, whereas dendritic cells expressed only
APG5. Furthermore, rodlet cells exhibited
immunoreactivity for S100 protein, IL-1{\beta},
NF-{\kappa}B, CD68, Nrf2, Ach, myostatin, SOX9, and
Iba-1. In contrast, stem cells displayed
immunoreactivity for Nrf2, myostatin, and SOX9. In
conclusion, the ovarian stroma of Molly fish
demonstrated a notable presence of immune cells,
indicating their active involvement in immune
reactions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Crisostomo:2024:RCP,
author = "Rafael Octavio Cris{\'o}stomo and Renzo
Pepe-Victoriano and Sheda M{\'e}ndez-Ancca and Abel
Walter Zambrano-Cabanillas and Olegario
Mar{\'\i}n-Machuca and Hernan Mauricio Perez and
V{\'\i}ctor Yana-Mamani and Mario Ruiz-Choque",
title = "Reproductive Conditioning of the \geoname{Peruvian}
Scallop \bioname{Argopecten purpuratus} in Different
Environments",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "9",
day = "24",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010009",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/9",
abstract = "Obtaining viable Argopecten purpuratus seeds faces
challenges, especiallyci the unpredictability of the
marine environment and high production costs in
hatcheries. However, improving the method of
``Broodstock Conditioning In Hatcheries'' is key to
ensure permanent seed supplies by minimizing the
dependence on marine conditions and by maximizing
economic viability in hatcheries. In an effort to
overcome these barriers, broodstock were conditioned
into two different environments: (a) Natural
Environment: Natural marine conditions located in
Bah{\'\i}a Inglesa, Atacama Region, Chile. (b)
Hatchery: Laboratory conditions to achieve gonadal
maturation, spawning induction, fertilization and
larval development. The purpose of this research was to
evaluate how the type of reproductive conditioning
affects the reproductive potential and nutritional
quality of the progeny. Both methods were successful at
inducing the necessary maturity for reproduction,
obtaining viable gametes and larvae. On the other hand,
it was observed that in the natural environment, the
oocytes and D larvae reached a greater size and
nutritional value, being the most significant
differences with (p {$<$} 0.05): the size of the D
larvae reached figures of 95.8 {\pm} 3.1 {\mu}m and
91.2 {\pm} 2.7 {\mu}m in the environment and hatchery,
respectively; the lipid content in dry mass was 25.2
{\pm} 3.1 mg g {$-$1} and 13.5 {\pm} 1.9 mg g {$-$1}
for the natural environment and hatchery, respectively.
Although quality indicators in hatcheries were slightly
lower compared to the natural environment, the
possibility of conditioning A. purpuratus broodstock
independently of environmental variability highlights
the importance of further optimizing broodstock
conditioning aspects in hatcheries that would allow
more predictable and sustainable production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Svenning:2024:ECC,
author = "Martin A. Svenning and Eigil T. Bj{\o}rvik and Jane A.
Godiksen and Johan Hammar and Jack Kohler and Reidar
Borgstr{\o}m and Nigel G. Yoccoz",
title = "Expected Climate Change in the \geoname{High Arctic}
--- Good or Bad for \geoname{Arctic} Charr?",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "8",
day = "23",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010008",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/8",
abstract = "Lakes in the High Arctic are characterized by their
low water temperature, long-term ice cover, low levels
of nutrients, and low biodiversity. These conditions
mean that minor climatic changes may be of great
importance to Arctic freshwater organisms, including
fish, by influencing vital life history parameters such
as individual growth rates. In this study, Arctic charr
sampled from two Svalbard lakes (78--{79$^\circ $} N)
over the period 1960--2008 provided back-calculated
length-at-age information extending over six decades,
covering both warm and cold spells. The estimated
annual growth in young-of-the-year (YOY) Arctic charr
correlated positively with an increasing air
temperature in summer. This increase is likely due to
the higher water temperature during the ice-free
period, and also to some extent, due to the winter air
temperature; this is probably due to thinner ice being
formed in mild winters and the subsequent earlier ice
break-up. However, years with higher snow accumulation
correlated with slower growth rates, which may be due
to delayed ice break-up and thus a shorter summer
growing season. More than 30\% of the growth in YOY
charr could be explained specifically by air
temperature and snow accumulation in the two Arctic
charr populations. This indicated that juvenile
Svalbard Arctic charr may experience increased growth
rates in a future warmer climate, although future
increases in precipitation may contradict the positive
effects of higher temperatures to some extent. In the
longer term, a warmer climate may lead to the complete
loss of many glaciers in western Svalbard; therefore,
rivers may dry out, thus hindering migration between
salt water and fresh water for migratory fish. In the
worst-case scenario, the highly valuable and attractive
anadromous Arctic charr populations could eventually
disappear from the Svalbard lake systems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:BVC,
author = "Lijuan Liu and Dongdong Jiang and Yan Ren and Cunbin
Shi and Yajun Wang and Jiyuan Yin and Qing Wang and
Defeng Zhang",
title = "The \bioname{Bacillus velezensis} {CYS06} Strain
Exhibits Promising Applications in Fighting Grass Carp
Bacterial Diseases",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "7",
day = "22",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010007",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/7",
abstract = "Aeromonas septicemia and columnaris disease are major
bacterial diseases in grass carp; however, the drugs
currently used to control these diseases pose
environmental and health risks. This study aimed to
screen for a probiotic Bacillus strain with
antagonistic activity to prevent and control bacterial
diseases in grass carp and to evaluate the
antimicrobial activities, biosafety, and biocontrol
effects of this strain. A Bacillus strain with
antagonistic activity against Aeromonas hydrophila,
obtained from grass carp intestines, was screened, and
the isolate CYS06 was identified by analyzing the 16S
rRNA and gyrA gene sequences. The antimicrobial
spectrum of the strain CYS06 was determined, and the
activities of amylase, cellulase, protease, and lipase
of the strain CYS06 were determined. The whole genome
of the strain CYS06 was sequenced using the nanopore
sequencing technology platform, followed by the
analysis of the antagonistic substance synthesis gene
clusters and CAZy enzyme gene families. The biosafety
of the strain CYS06 was evaluated via intraperitoneal
injection into healthy grass carp. After the strain
CYS06 was fed to the grass carp, its biological control
effect on this fish was evaluated through artificial
infection experiments. The strain CYS06 was identified
as Bacillus velezensis, based on molecular
identification, which shows broad antimicrobial
activity against various fish pathogens. The strain
CYS06 secretes amylase, cellulase, protease, and
lipase. The genome size of the strain CYS06 is
3,914,159 bp, and it contains eight antagonistic
substance synthesis gene clusters and many CAZy
enzymes. The strain CYS06 exhibits high biological
safety for grass carp, based on the challenge test.
Feeding grass carp with the strain CYS06 for 4 weeks
significantly enhanced the resistance of the fish to A.
hydrophila. Strain CYS06 could inhibit the growth of
Flavobacterium columnare under co-culture and reduce
the amount of F. columnare adherence on the gills of
grass carp, indicating that CYS06 has good potential
for the prevention and control of columnaris disease.
In conclusion, we isolated an antagonistic probiotic
strain, CYS06, which exhibits a biological control
effect on septicemia and columnaris disease caused by
Aeromonas spp. and F. columnare in grass carp,
respectively. This strain contains many antagonistic
substance synthesis-related gene clusters and holds the
potential to degrade various types of carbohydrates. As
a biological control agent, the strain CYS06 exhibits
significant potential for the prevention and control of
bacterial diseases in grass carp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2024:IBC,
author = "Yiqian Xu and Junren Xue and Hongbo Liu and Tao Jiang
and Xiubao Chen and Jian Yang",
title = "Identification of {``Bathed''} \geoname{Chinese}
Mitten Crabs (\bioname{Eriocheir sinensis}) Using
Geometric Morphological Analysis of the Carapace",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "6",
day = "21",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010006",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/6",
abstract = "To confirm whether Chinese mitten crabs (Eriocheir
sinensis), commonly known as hairy crabs or river
crabs, in non-Yangcheng Lake areas undergo
morphological convergence with the original crabs in
the Yangcheng Lake purse seine and high-standard
modified aquaculture ponds after being ``introduced''
or ``bathed''-cultured, we employed a geometric
morphometrics approach. This approach allowed us to
compare and analyze the dynamic changes in the carapace
morphology of both the original and ``introduced''
crabs in Yangcheng Lake and high-standard ponds in
Kunshan City at 0, 7, 14, and 30 days after ``bathing''
culture. The geometric morphological analysis of the
carapace was conducted using a system of 35 established
landmarks. The stepwise discriminant analysis of the
relative distortion score revealed morphological
differences between ``introduced'' and resident
original crabs in the Yangcheng Lake area and in
high-standard modified ponds after 7, 14, and 30 days
of bathing culture. The accuracy of the discriminant
analysis was 100\%. The results of the geometric
morphological visualization demonstrated that the
carapaces of the bathed crabs underwent adaptive
changes in the water. However, even after one month of
bathing culture, the ``introduced'' crabs in lakes and
ponds could not reach the morphological characteristics
of the original crabs, i.e., their carapaces did not
exhibit the characteristics of the original crabs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sanchez-Paz:2024:SEP,
author = "Arturo S{\'a}nchez-Paz and Trinidad
Encinas-Garc{\'\i}a and Fernando Mendoza-Cano",
title = "In Silico Evaluation of the {PCR} Performance of
Different Tests for Detection of {WSSV}",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "5",
day = "20",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010005",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/5",
abstract = "In this study, the primers of different protocols for
the detection of White Spot Syndrome Virus (WSSV) were
analyzed in silico to evaluate their potential
performance in PCR. As with any biological entity, this
virus evolves constantly. Thus, this analysis showed
that a few primers, including those recommended by the
World Organization for Animal Health (WOAH), might
mismatch with some isolates of WSSV, specially with
isolates more recently sequenced. Furthermore, a set of
primers recommended by WOAH, showed the potential to
self-dimer and form hairpin loop structures, which
could affect the efficiency of PCR, resulting in an
inaccurate diagnostic result. From our perspective, and
considering the evolutionary trajectory of this virus,
it may be time for the WOAH to update the PCR protocols
recommended for WSSV detection, which remains as a
highly prevalent and lethal virus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Valic:2024:MAT,
author = "Damir Vali{\'c} and Matej Kristan Mirkovi{\'c} and
Vi{\v{s}}nja Besendorfer and Emin
Teskered{\v{z}}i{\'c}",
title = "Molecular Analysis of Two Endemic \bioname{Squalius}
Species: Evidence for Intergeneric Introgression among
{Cyprinids} and Conservation Issues",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "4",
day = "20",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010004",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/4",
abstract = "Conservation of indigenous species, especially endemic
ones, is of the utmost importance. Morphological
determination of species is usually not sufficient;
therefore, molecular phylogenetic analyses of the
Illyrian chub, Squalius illyricus, and the Zrmanja
chub, Squalius zrmanjae, from the Krka River were
performed. For the genetic characterization of the
mitochondrial gene cytochrome b and the non-coding
nuclear region Cyfun P, 15 specimens from each species
were subjected to analysis. The obtained sequences were
aligned with similar ones from GenBank to determine the
taxonomic and phylogenetic position of these species.
The obtained molecular results imply that S. zrmanjae
from the Krka River has a nuclear region that resembles
Dalmatian rudd, Scardinius dergle. This result implies
an introgression event and the transfer of genetic
information between the two genera. The investigated
species are on the IUCN Red List of Threatened Species,
their biological data are scarce, and further
investigation and protection are needed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Horka:2024:ETB,
author = "Petra Horka and Monika Vlachova",
title = "The Effect of Turbidity on the Behavior of Bleak
(\bioname{Alburnus alburnus})",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "3",
day = "20",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010003",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/3",
abstract = "In group-living species, social interactions with
conspecifics play a crucial role in group formation and
the ability to make consensus decisions, with
far-reaching consequences for ecological and
evolutionary processes in natural populations.
Individual recognition and partner preferences based on
social familiarity are important mechanisms driving a
range of interactions between individual fish and
social structure in fish populations. However, the
social interactions of gregarious species are also
influenced by the ecological environment experienced by
individuals. This study aimed to determine how fish
shoals' structure is shaped by increased turbidity, a
typical environmental constraint in anthropogenically
impacted rivers. A freshwater, shoal-forming, visually
orientated pelagic fish---bleak (Alburnus
alburnus)---was used as the model organism. The
behavior of 40 individuals at three different turbidity
levels (0 NTU, 30 NTU, 60 NTU) was tested in the
laboratory experiment. Specifically, the aim was to
determine if the turbidity reduces between individual
distances in response to the deteriorated visual
conditions. The results showed that bleaks increased
the compactness of the shoal even at the medium
turbidity level (30 NTU), and compactness further
increased with turbidity. Such results indicate that
turbidity is an important phenomenon influencing the
structure of shoals and ultimately an ecological
process in natural fish populations in ecosystems
affected by increasing turbidity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Herdiana:2024:UCS,
author = "Yudi Herdiana and Budy Wiryawan and Sugeng Hari Wisudo
and James Richard Tweedley and Irfan Yulianto and Heidi
Retnoningtyas and Neil Randell Loneragan",
title = "Untangling the Complexity of Small-Scale Fisheries:
Building an Understanding of Grouper-Snapper Fisheries
Dynamics in \geoname{Saleh Bay, West Nusa Tenggara,
Indonesia}",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "2",
day = "19",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010002",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/2",
abstract = "Small-scale tropical fisheries are complex systems
that utilize multiple fishing gears to target various
species. In this study, we investigated small-scale
reef and demersal fisheries in Saleh Bay, Indonesia,
using catch and effort data from 2016 to 2019, where
57.7 percent of the catch was grouper (serranids) and
snapper (lutjanids). Despite the complexity (75
documented species and eight fishing methods), this
fishery is characterized by the catches of four
dominant species: leopard coral grouper (Plectropomus
leopardus), orange-spotted-grouper (Epinephelus
coioides), spotted coral grouper (P. maculatus), and
malabar blood snapper (Lutjanus malabaricus). The
species caught varied among fishing methods. Over 90\%
of the catch was attributed to three primary fishing
methods: bottom longline, speargun, and handline.
Multivariate analyses found that fishing depth, season,
and/or year significantly influenced the catch
composition for each of these fishing methods. Fishing
activities exhibit a temporal pattern influenced by
monsoonal seasons. Results also suggest that fishers
employ specialized fishing tactics by targeting
high-value species to maximize their profits. This
study recommends a management strategy of focusing on
monitoring and managing the three main fishing gears
and four important species during their peak seasons to
reduce some of the complexity and management costs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jiang:2024:PTM,
author = "Zheng-Ting Jiang and Gang Shi and Dong-Neng Jiang and
Yu Li and Yuan-Qing Huang and Hong-Juan Shi and
Guang-Li Li",
title = "Preliminary Trial of Male to Female Sex Reversal by
17$ \beta $-Estradiol in Combination with Trilostane in
Spotted Scat (\bioname{Scatophagus argus})",
journal = j-FISHES,
volume = "9",
number = "1",
pages = "1",
day = "19",
month = dec,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9010001",
ISSN = "2410-3888",
bibdate = "Tue Dec 26 13:36:16 MST 2023",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/1/1",
abstract = "The spotted scat (Scatophagus argus) is an important
ornamental species with sexually biased ornamental
values that favor males. Therefore, it makes sense to
breed mono-male fingerlings as ornamental fish. The
spotted scat has an XX/XY sex determination system;
therefore, the first step in producing genetically
all-male offspring should be the induction of a fertile
sex-reversed XY pseudo-female, which would then be
mated with a normal XY male to produce a YY super-male.
However, the XY pseudo-female produced by estradiol (E
2) treatment failed due to ovarian malformations. Here,
male to female sex reversal was induced in spotted scat
through a combination of E 2 and an androgen inhibitor
(trilostane, TR). Spotted scat fingerlings of
approximately 2.8--3.5 cm were fed a diet containing
both E 2 (300 {\SGMLmicro}g/g) and TR (300
{\SGMLmicro}g/g). The fish were treated for 90 days and
then fed a normal diet until they reached one year of
age, when they were sampled. Twenty-eight treated XY
individuals were identified using sex-linked markers.
According to their gonadal histological
characteristics, these treated XY fish could be divided
into three groups: males with testes (n = 21), intersex
individuals with ovaries--testes (n = 3), and fully
sex-reversed individuals with ovaries (n = 4). All
treated XX fish (n = 8) developed into normal females.
There were no obvious abnormalities in the ovaries of
the XY sex-reversed fish compared to the treated XX
fish. Serum 11-ketotestosterone (11-KT) levels were
significantly lower in XY-reversed females and treated
XX females than in XY males. Compared with XY male
fish, the expression of female (42sp50, foxl2, figla,
zar1, and zp2) and male (dmrt1, gsdf, amh and cyp11b2)
biased genes was up- and down-regulated, respectively,
in the gonads of XY-completely sex-reversed fish.
Immunohistochemical results indicate that 42Sp50 was
expressed in oocytes of XY-partially and completely
sex-reversed fish, while strong Gsdf signals were
mainly detectable in testicular somatic cells of
XY-partially sex-reversed fish and XY male fish. XY
females were successfully produced by the combined
treatment of E 2 and TR in the spotted scat. Their
fertility will be tested in the future.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Roubie:2024:TMD,
author = "Eleni Roubie and Sotirios Karavoltsos and Aikaterini
Sakellari and Nikolaos Katsikatsos and Manos Dassenakis
and Persefoni Megalofonou",
title = "Trace Metals Distribution in Tissues of 10 Different
Shark Species from the \geoname{Eastern Mediterranean
Sea}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "77",
day = "16",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020077",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/77",
abstract = "As long-living apex predators, sharks tend to
bioaccumulate trace metals through their diet. The
distribution of Al, As, Cd, Co, Cr, Cs, Cu, Fe, Mn, Ni,
Pb, V, Zn and Hg in different tissues (muscle, liver,
heart, gills and gonads) of large-size (58--390 cm)
sharks, some of which rare, of the eastern
Mediterranean Sea was studied. Trace metals analyses in
samples originating from ten different Chondrichthyes
species were performed by inductively coupled
plasma--mass spectrometry (ICP-MS) and Cold Vapor
Atomic Absorption Spectrometry (CVAAS) for Hg. Data on
trace metal levels are for the first time reported
herewith for the species O. ferox and H. nakamurai.
Higher median concentrations of trace metals were
generally determined in the liver. The concentrations
of Hg, Cs and As in the muscle increased proportionally
with body length. Statistically significant differences
between sexes were recorded for Hg, Cr, Ni and As (p =
0.015) in the muscle tissues of P. glauca. Muscle
tissue Hg concentrations exceeded the EU maximum limit
(1 {\mu}g g {$-$1} wet weight) in 67\% of the
individuals sampled, with the highest concentrations
detected in O. ferox and S. zygaena, whereas regarding
Pb (limit 0.30 {\mu}g g {$-$1} ww), the corresponding
percentage was 15\%. Arsenic concentrations were also
of concern in almost all shark tissues examined.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Abdelbaky:2024:ICV,
author = "Shimaa A. Abdelbaky and Zakaria M. Zaky and Doha Yahia
and Mohamed H. Kotob and Mohammed A. Ali and Mohammed
Aufy and Alaa El-Din H. Sayed",
title = "Impact of \bioname{Chlorella vulgaris} Bioremediation
and Selenium on Genotoxicity, Nephrotoxicity and
Oxidative\slash Antioxidant Imbalance Induced by
Polystyrene Nanoplastics in \geoname{African} Catfish
(\bioname{Clarias gariepinus})",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "76",
day = "15",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020076",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/76",
abstract = "Contamination of the environment with nano- and
microplastic particles exerts a threatening impact on
the aquatic ecosystems and sustainable catfish
aquaculture. The presence of nanoplastics has been
found to have a detrimental impact on both aquatic and
terrestrial ecosystems. The present study examines the
effect of polystyrene nanoplastics (PS NPs) on the DNA,
erythrocytes, oxidative status and renal histology of
catfish, in addition to the potential protective
effects of Chlorella vulgaris bioremediation and
selenium to hinder this effect. Six equal groups of
fish were used as follows: Group 1 served as a control
group and received water free from PS NPs; Group 2 was
exposed to PS NPs at a concentration of 5 mg/L; Group 3
was exposed to PS NPs (5 mg/L) + selenium (1 mg/kg
diet); Group 4 was exposed to PS NPs (5 mg/L) + C.
vulgaris (25 g/kg diet); Group 5 was supplemented with
C. vulgaris (25 g/kg diet); and Group 6 was
supplemented with selenium (1 mg/kg diet). The exposure
period was 30 days. The results indicated that PS NPs
induced oxidative stress by significantly elevating
malondialdehyde activities and slightly reducing
antioxidant biomarkers, resulting in DNA damage,
increased frequency of micronuclei, erythrocyte
alterations, and numerous histopathological alterations
in kidney tissue. Selenium and C. vulgaris
significantly ameliorated the oxidative/antioxidant
status, reducing DNA damage, micronucleus frequency,
erythrocyte alterations, and improving the morphology
of kidney tissue. Nevertheless, further research is
needed for a profound understanding of the mechanism
behind the toxicity of nano-microplatics in aquatic
systems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Negrini:2024:PRF,
author = "Celma Negrini and Caio Henrique do Nascimento Ferreira
and Rafael Ortiz Kracizy and Rosane Lopes Ferreira and
Luana Costa and Marlise Teresinha Mauerwerk and Milena
Cia Retcheski and Luisa Helena Cazarolli and Wilson
Rog{\'e}rio Boscolo and Eduardo Luis Cupertino
Ballester",
title = "Partial Replacement of Fish Meal with Protein
Hydrolysates in the Diet of \bioname{Penaeus vannamei}
({Boone}, 1934) during the Nursery Phase",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "75",
day = "15",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020075",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/75",
abstract = "The objective was to evaluate the effects of partial
replacement of fish meal with protein hydrolysates and
a commercial product in the diets of Penaeus vannamei
post-larvae on zootechnical performance, proximate
chemical composition, digestive enzyme activity, and
total hemocyte count. The experiment was conducted in a
clear water recirculation system comprising 24
experimental units, each with 30 shrimp with an average
weight of 0.2 g. The treatments were control, chicken
protein hydrolysate (CPH), enzymatic hydrolysate of
chicken feathers, Aquabite {\reg}, CPH + maltodextrin,
and CPH + yeast, with four replicates each. The
inclusion level of the different protein sources
evaluated was 6\%. At the end of the experiment, all
shrimp were counted, weighed, and measured to determine
the zootechnical performance. The body and feed
chemical compositions, as well as the enzymatic
activities of the hepatopancreas and the total hemocyte
count in the hemolymph, were analyzed. The data
obtained were subjected to ANOVA and Tukey's test.
There was no statistical difference between the
treatments in any of the analyzed parameters. The
results showed that all the evaluated protein
hydrolysates can be used as partial substitutes for
fish meal in P. vannamei diets during the nursery
phase, maintaining equivalent and adequate digestive
enzyme activities, health, growth, and body composition
of the shrimp, in addition to being ecologically
sustainable ingredients.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Papadopoulos:2024:IWN,
author = "Dimitrios K. Papadopoulos and Athanasios Lattos and
Ioanna Chatzigeorgiou and Aphrodite Tsaballa and
Georgios K. Ntinas and Ioannis A. Giantsis",
title = "The Influence of Water Nitrate Concentration Combined
with Elevated Temperature on Rainbow Trout
\bioname{Oncorhynchus mykiss} in an Experimental
Aquaponic Setup",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "74",
day = "13",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020074",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/74",
abstract = "Intensive recirculating systems are a fast-developing
sector of aquaculture. While several warm-water fish
have been reared in aquaponics, almost no data are
available for cold-water species. The determination of
nitrate toxicity thresholds in recirculating
aquaculture is crucial. Different pollutants are
typically more toxic at elevated temperatures. We
investigated the performance of Oncorhynchus mykiss
under two different nitrate levels and two
temperatures. We applied a 2 $ \times $ 2 factorial
design, where fish (9.78 {\pm} 0.51 g) were exposed to
nitrate concentrations of 40 or 110 mg/L NO 3 {$-$} and
to temperatures of 17 {$^\circ $}C or 21 {$^\circ $}C
for 20 days. This study focused on understanding the
physiological responses of rainbow trout to relatively
low nitrate levels under heat stress in order to
investigate the feasibility of integrating this species
into commercial aquaponics. The growth, condition, and
expression of genes involved in metabolism, heat shock,
antioxidant, and immune response were assessed in the
liver, together with the activities of enzymes related
to glucose and fatty acid metabolism. High nitrate
levels at 17 {$^\circ $}C affected the condition but
did not alter growth, leading to increased glycolytic
potential and, occasionally, a greater reliance on
lipid oxidation. Antioxidant defense was mainly induced
due to high nitrates and the similar expression
patterns of antioxidant genes observed under high
nitrate at both 17 {$^\circ $}C and 21 {$^\circ $}C.
Warm exposure decreased condition and growth, leading
to greatly reduced glucokinase transcription,
irrespective of the nitrate levels. Exposure to 21
{$^\circ $}C and high nitrate led to equivalent growth
and condition as well as to a milder inflammatory
response combined with metabolic readjustments
(enhancement of glycolytic and lipid oxidation
pathways) compared to the low nitrates at 21 {$^\circ
$}C. Based on the results, rearing at a temperature
close to 21 {$^\circ $}C should be avoided for
fingerling growth, while NO 3 {$-$} concentration until
110 mg/L may not have severe impacts on fingerling
health and growth at 17 {$^\circ $}C. In addition,
rainbow trout fingerlings can tolerate a 20-day
exposure at 21 {$^\circ $}C and NO 3 {$-$} up to 110
mg/L. Additional factors should always be considered,
such as specific water quality parameters, for a
comprehensive approach to assessing the feasibility of
rainbow trout aquaculture in aquaponics.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Negrini:2024:DPH,
author = "Celma Negrini and Caio Henrique do Nascimento Ferreira
and Rafael Ortiz Kracizy and Rosane Lopes Ferreira and
Luana Cardoso dos Santos and Milena Cia Retcheski and
Marlise Teresinha Mauerwerk and Luisa Helena Cazarolli
and Wilson Rog{\'e}rio Boscolo and Eduardo Luis
Cupertino Ballester",
title = "Different Protein Hydrolysates Can Be Used in the
\bioname{{Penaeus} vannamei} ({Boone}, 1934) Diet as a
Partial Replacement for Fish Meal during the Grow-Out
Phase",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "73",
day = "12",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020073",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/73",
abstract = "This study evaluated the inclusion of protein
hydrolysates and a commercial product as a partial
replacement for fish meals in the diet of Pacific white
shrimp (Penaeus vannamei) during the grow-out phase. A
recirculation system with 24 experimental units and a
biological filter was used. The experimental design was
completely randomized with six treatments: control;
chicken protein hydrolysate (CPH); enzymatic
hydrolysate of chicken feathers; Aquabite {\reg}; CPH +
maltodextrin; CPH + yeast; and four replicates. After
50 days, the zootechnical performance and animal
welfare parameters, centesimal carcass composition,
digestive enzyme activity, and hemocyte count were
evaluated. The treatments did not affect the
zootechnical performance and total hemocyte count of
the animals (p {$>$} 0.05). The evaluation of the
antenna length indicated that all animals were in good
health. The antenna length in treatment T5 was
significantly greater (p {$>$} 0.05) than that in T4
and similar to that in treatments T3 and T6,
demonstrating a positive influence of dietary protein
hydrolysates. Concerning animal welfare, dietary
protein hydrolysates influenced the length of the
antenna (p {$<$} 0.05). The antenna length associated
with different treatments indicated that the animals
were in good welfare conditions in the production
environment. With respect to body chemical composition,
dry matter, ether extract, and ash were affected by the
treatments (p {$<$} 0.05). Regarding the analysis of
digestive enzymes, the treatments influenced the
activities of amylase and trypsin (p {$<$} 0.05). The
performance of the animals was satisfactory under all
treatments, including enzymatic activity, demonstrating
the possibility of using hydrolysates.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yu:2024:MTC,
author = "Linghui Yu and Weijun Yin and Senrong Han and Tanjun
Zhao and Zhenlin Hao and Donghong Yin and Yaoyao Zhan
and Yaqing Chang",
title = "Morphological Trait Correlations, Gonadal Development
Characteristics and Pleopod Nutrient Compositions of
the Whelk \bioname{Volutharpa perryi perryi}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "72",
day = "11",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020072",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/72",
abstract = "The aim of this study was to clarify the
``morphological-trait--body weight'' correlation,
gonadal development characteristics, and pleopod (main
edible part) nutrient composition of the whelk
(Volutharpa perryi perryi). Live body mass (BM), soft
tissue mass (STM), and eight other morphological traits
of the whelk were measured, and path coefficients,
correlation indices (R 2), and coefficients of
determination were then calculated. Gonadal development
characteristics were investigated by histological
observation. Pleopod nutrient composition was analyzed
by standard biochemical assays. The results indicated
that (1) shell aperture width (SAW) and body whorl
height (BWH) were positively correlated with both live
BM and STM (p {$<$} 0.01), and shell height (SH) was
positively correlated with both live BM and STM (p
{$<$} 0.01) in male whelks; (2) similar gonadal
development characteristics were observed in both
female and male whelks; and (3) pleopod nutrient
composition was consistent in both female and male
whelks, whereas sex-specific variation in pleopod
nutrient content was observed in the whelks. The
observations in this study will provide theoretical
support for the development of the whelk aquaculture
industry.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Porto:2024:EPF,
author = "L{\'\i}via de A. Porto and Yhago P. A. S. Assis and
Matheus P. S. Amorim and Paulo E. C. M. de Oliveira and
Alessandro L. Paschoalini and Nilo Bazzoli and Ronald
K. Luz and Gisele C. Favero",
title = "Effects of Prolonged Fasting and Refeeding on
Metabolic, Physiological, Tissue, and Growth
Performance Adjustments in \bioname{Colossoma
macropomum}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "71",
day = "10",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020071",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/71",
abstract = "Fish can tolerate prolonged periods of fasting more
easily than endothermic organisms. However, these
fasting periods are associated with pronounced lipid
and protein catabolism and body weight loss. We
evaluated the use of body reserves, growth performance,
and the histology of the intestines and muscles of
Colossoma macropomum subjected to prolonged fasting for
45 days and refeeding for 14 days. We used 66 juvenile
C. macropomum (71.78 {\pm} 10.75 g) distributed in 10
tanks of 100 L in a recirculating aquaculture system
(RAS) and kept 6 fish in a separate tank, considered
the basal group. The fish were divided into two groups:
fed (continuously fed for 59 days) and fasted/refed
(subjected to fasting for 45 days and subsequently
refed for 14 days). The tambaqui juveniles showed the
mobilization of their body reserves during 45 days of
fasting but with a large deficit in their growth
performance. The 14-day refeeding period was sufficient
for fish to restore their energy but insufficient for
recovering most growth parameters.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Imsland:2024:EDF,
author = "Albert Kjartan Dagbjartarson Imsland and Hj{\"o}rtur
Meth{\'u}salemsson",
title = "The Effects of Different Feed Ration Levels on Growth,
Welfare Rating, and Early Maturation in Juvenile
\geoname{Atlantic} Salmon (\bioname{Salmo salar})",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "70",
day = "10",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020070",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/70",
abstract = "To investigate the possible effect of different feed
ration levels on the growth, welfare, and early
maturation of juvenile Atlantic salmon, 450 salmon parr
with a mean ({\pm}standard error) initial weight of
51.6 g ({\pm}0.8) were reared in triplicate under three
different feed ration levels for five months. The
control group (100r) was fed every day, the 50r group
was fed every other day, and the 33r group was fed
every third day. In every group, 75 fish (half of the
group) were individually tagged for monitoring of
growth. The number of fin wounds was used as the
welfare indicator, and to inspect the development of
maturation, all fish were euthanized, and development
of the gonads was monitored by visual inspection at the
termination of the trial. The control group (100r)
showed a significantly higher specific growth rate
(0.90\% day {$-$1}) compared to the lower fed groups
(50r, 0.67\% day {$-$1} and 33r, 0.49\% day {$-$1});
however, the growth difference was 21--24\% less than
expected solely on the difference in the amount of feed
given to each group. The 100r group showed the highest
welfare rating, and the 33r group the lowest possible,
indicating more aggressive behaviour and fin biting due
to feed restriction in the 33r group. No difference (p
{$>$} 0.45) was found in the development of maturation
in females, but the combined numbers of males in
maturity stages 2--5 showed an overall trend towards
slower maturation in the 33r group compared to the 100r
group. Although the present findings on the development
of sexual maturation were subtle due to the limited
time frame of the trial, the findings offer a
foundation for future investigation into the
relationship between the feed ration level and the
development of sexual maturation in the rearing of
juvenile Atlantic salmon.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cotou:2024:GPE,
author = "Efthimia Cotou and Helen Miliou and Evanthia
Chatzoglou and Eirini Schoina and Nektarios Politakis
and Dimitra Kogiannou and Eleni Fountoulaki and
Afrodite Androni and Aggeliki Konstantinopoulou and
Georgia Assimakopoulou and Cosmas Nathanailides",
title = "Growth Performance and Environmental Quality Indices
and Biomarkers in a Co-Culture of the
\geoname{European} Sea Bass with Filter and Deposit
Feeders: a Case Study of an {IMTA} System",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "69",
day = "08",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020069",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/69",
abstract = "This study aimed to evaluate the efficiency of an
integrated multi-trophic aquaculture (IMTA) system
comprising co-cultured fed fish and organic extractive
species representing three distinct trophic levels as
well as the impact and potential utilization of two
commercially available fish feeds made up of 35\% fish
meal (FM) and 20\% fish meal (LFM) ingredients, using a
multi-indicator assessment approach. Significant
alterations were observed in growth performance
indicators (GPIs), water and sediment quality indices,
toxicity tests and biomarkers within the IMTA system.
The fish survival, weight gain (WG), and specific
growth rate (SGR) were higher in the IMTA system with
significantly lower feed conversion ratios (FCRs) and
higher feed efficiency (FE) in comparison to the fed
fish monoculture system. Yet, organic filter feeders
displayed 100\% survival, and increased shell growth,
while deposit feeders exhibited successful survival and
significant weight gain. In the comparison between
FM-IMTA and LFM-IMTA, fed fish in FM-IMTA showed higher
WG, SGR, and FE with lower FCR. Environmental
parameters like temperature, oxygen, and nutrient
concentrations fluctuated but generally improved in the
IMTA system, indicating lower mesotrophic conditions.
Sediment fatty acid profiles differed between systems
and toxicity assessments, which suggested a lower
impact in IMTA and FM-IMTA systems. The sediment
microbial community displayed high similarity within
IMTA systems and between FM-IMTA and LFM-IMTA. These
findings underscore the potential of IMTA systems for
sustainable aquaculture, emphasizing improved growth
performance and reduced environmental impact,
particularly when using fish meal feeds.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Monahan:2024:CMA,
author = "Dylan Monahan and Jeff S. Wesner and Stephanie M.
Parker and Hannah Schartel",
title = "Correction: {Monahan et al., \booktitle{Spatial
Patterns in Fish Assemblages across the National
Ecological Observation Network (NEON): The First Six
Years}. Fishes 2023, {\bf 8}, 552}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "68",
day = "08",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020068",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
note = "See \cite{Monahan:2023:SPF}.",
URL = "https://www.mdpi.com/2410-3888/9/2/68",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2024:ECC,
author = "Yijie Wu and Liting Chen and Xin Yan and Jun Xiao and
Zhirui Ma and Zhanyang Tang and Zhongbao Guo and Liping
Li and Guixiang Tong and Honglian Tan and Fuyan Chen
and Xinxian Wei and Ting Huang and Yongju Luo",
title = "The Effect of Copper--Cadmium Co-Exposure and Hormone
Remediation on the Ovarian Transcriptome of
\geoname{Nile} Tilapia (\bioname{Oreochromis
niloticus})",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "67",
day = "08",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020067",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/67",
abstract = "The escalating problem of copper (Cu) and cadmium (Cd)
pollution in aquatic environments poses a significant
threat to the ovarian tissue and reproductive capacity
of fish, hindering the development of the aquaculture
industry. However, the combined effects of Cu and Cd on
fish gonadal development remain unclear. In this study,
the fish species Nile tilapia was stressed with rearing
water containing 300 {\mu}g/L Cu 2+ and 100 {\mu}g/L Cd
2+ for 30 days, followed by an intraperitoneal
injection of luteinizing hormone-releasing hormone
(LHRH-{\alpha}) and human chorionic gonadotropin (HCG)
at various concentrations. We investigated the ovarian
transcriptome profiles before and after injection.
Prior to injection, combined treatment with Cu and Cd
resulted in reproductive dysfunction and metal ion
imbalance in tilapia. Transcriptomic profiling revealed
differential gene annotation concentrated in the MAPK
signaling pathway and regulation of GTPase activity.
Post-injection, all concentrations of LHRH-{\alpha} and
HCG groups showed an upregulated gonadosomatic index
(G.S.I) and higher levels of vitellogenin (VTG),
gonadotropin-releasing hormone (GnRH), gonadotropin
(GTH), and estrogen (E2) in serum compared to the
negative control group. Transcriptomic analysis
revealed alterations in various ovarian signaling
pathways, preliminarily revealing the in vivo molecular
mechanisms and differences in LHRH-{\alpha} and HCG.
The findings from this study could help us better
understand how to counteract the effects of combined Cu
and Cd exposure on tilapia ovarian development, which
has significant implications for the Nile tilapia
aquaculture industry.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lu:2024:SIS,
author = "Zhengwei Lu and Liming Song and Keji Jiang",
title = "Status Identification in Support of Fishing Effort
Estimation for Tuna Longliners in Waters near the
\geoname{Marshall Islands} Based on {AIS} Data",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "66",
day = "08",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020066",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/66",
abstract = "Visualising the fishing behaviour of vessels and
quantifying the spatial distribution of fishing effort
is the scientific basis for assessing and managing
fisheries resources. The information on the dynamics of
fishing vessel voyages provided by the automatic
identification system (AIS) of vessels serves as
high-precision fishery data and provides a means of
quantifying fishing effort with high spatial and
temporal resolution in the tuna longline fishery. Based
on the AIS data of five tuna longliners operating in
the waters near the Marshall Islands from 2020 to 2021,
this study used three methods, namely the threshold
screening method, the construction of a BP neural
network and the support vector machine (SVM) to
identify the fishing and non-fishing status of the tuna
longliners, respectively. This study investigates the
status identification and fishing effort estimation of
the tuna longliner (VESSEL A) in 2021 based on the
constructed optimal model, and spatial correlation
analyses are performed between the fishing effort
estimated in hours based on AIS data and in hooks based
on fishing logbook data, by month. The results showed
(1) the recognition accuracy of the threshold screening
method is 89.9\%, the recognition accuracy of the BP
neural network classification model is 95.11\%, the
kappa coefficient is 0.51, the recognition accuracy of
the SVM classification model is 95.74\% and the kappa
coefficient is 0.52; (2) in comparison, the SVM
classification model performs better than the other two
status identification methods for tuna longliners; and
(3) the correlation coefficients between the two types
of effort of VESSEL A were greater than 0.79 on all
fishing months, indicating that there was no
significant difference in the spatial and temporal
distribution between the two types of effort. This
study suggests that the SVM model can be used to
identify the status and estimate the fishing effort of
longliners.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Poisson:2024:CPL,
author = "Fran{\c{c}}ois Poisson",
title = "Consumption of Post-Larval Swordfish (\bioname{Xiphias
gladius}) by Dolphinfish (\bioname{Coryphaena
hippurus}): New Ecological Insights into Both Species
in the \geoname{Tyrrhenian Sea}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "65",
day = "06",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020065",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/65",
abstract = "Knowledge of post-larval swordfish (Xiphias gladius)
ecology in the world's oceans is incomplete as they are
rarely found in ichthyoplankton samplings or commercial
catches and individuals are difficult to observe in the
marine ecosystem. Analyses of stomach contents of apex
predators can provide otherwise unobtainable ecological
insights. Two well-preserved bills of post-larval
swordfish were identified among the partially digested
stomach contents of an adult male dolphinfish
(Coryphaena hippurus) caught in the northern Tyrrhenian
Sea. The lower jaw to fork lengths of the two predated
swordfish were estimated at, respectively, 18 and 22
cm, corresponding to 81- and 99-day-old swordfish
hatched in the 2022 spawning season. This analysis and
recent information on the reproductive dynamics of
swordfish in the same area reported in the literature
shed light not only on the early stages of swordfish
growth but also on dolphinfish ecology in the
Tyrrhenian Sea. The two species share the same habitat
and are both caught with commercial pelagic surface
longlines. Environmental shifts associated with climate
change could unpredictably modify the reproduction
dynamics, growth, recruitment and distribution of both
species. This knowledge is important for the
sustainable management of regional fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xie:2024:DLB,
author = "Mingyang Xie and Bin Liu and Xinjun Chen and Wei Yu
and Jintao Wang",
title = "Deep Learning-Based Fishing Ground Prediction Using
Asymmetric Spatiotemporal Scales: a Case Study of
\bioname{Ommastrephes bartramii}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "64",
day = "04",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020064",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/64",
abstract = "Selecting the optimal spatiotemporal scale in fishing
ground prediction models can maximize prediction
accuracy. Current research on spatiotemporal scales
shows that they are symmetrically distributed, which
may not capture specific oceanographic features
conducive to fishing ground formation. Recent studies
have shown that deep learning is a promising research
direction for addressing spatiotemporal scale issues.
In the era of big data, deep learning outperforms
traditional methods by more accurately and efficiently
mining high-value, nonlinear information. In this
study, taking Ommastrephes bartramii in the Northwest
Pacific as an example, we used the U-Net model with sea
surface temperature (SST) as the input factor and
center fishing ground as the output factor. We
constructed 80 different combinations of temporal
scales and asymmetric spatial scales using data in
1998--2020. By comparing the results, we found that the
optimal temporal scale for the deep learning fishing
ground prediction model is 15 days, and the spatial
scale is {0.25$^\circ $} $ \times $ {0.25$^\circ $}.
Larger time scales lead to higher model accuracy, and
latitude has a greater impact on the model than
longitude. It further enriches and refines the criteria
for selecting spatiotemporal scales. This result
deepens our understanding of the oceanographic
characteristics of the Northwest Pacific environmental
field and lays the foundation for future artificial
intelligence-based fishery research. This study
provides a scientific basis for the sustainable
development of efficient fishery production.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vijayaram:2024:UAA,
author = "Srirengaraj Vijayaram and Einar Ring{\o} and Hamed
Ghafarifarsani and Seyed Hossein Hoseinifar and Saman
Ahani and Chi-Chung Chou",
title = "Use of Algae in Aquaculture: a Review",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "63",
day = "01",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020063",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/63",
abstract = "The utilization of algae in aquaculture is
environmentally friendly, safe, and cost-effective and
can effectively substitute for fish meal and fish oil
in aquatic feeds. Incorporating algae as dietary
supplements leads to significant enhancements in
aquatic animals' health and also improves the aquatic
ecosystem. Algae are rich sources of nutrients and
serve as the foundational food source in the aquatic
food chain. Currently, 40 different algae species are
employed in aquaculture. Furthermore, algae contributes
to elevating the overall quality of aquatic feed
products. Aquaculture stands as the most vital food
production sector globally; however, challenges such as
infection outbreaks and aquatic environmental pollution
pose significant threats to the sustainable growth of
this industry. An alternative strategy for mitigating
environmental issues and improving aquatic production
involves the utilization of algae. The novelty in the
applications of algae in aquaculture stems from their
multifaceted roles and benefits, such as their capacity
to improve water quality, serve as nutrient-rich feed
supplements, and enhance the overall health and
productivity of aquatic species. These versatile
applications of algae represent a fresh and innovative
approach to sustainable aquaculture practices. This
review furnishes insights into the use of algae, algae
extracts, or components derived from algae to enhance
water quality. Additionally, it covers the utilization
of algae-based feed supplements, boosting of the immune
system, enhanced growth performance, and disease
resistance in aquatic animals.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:AIE,
author = "Fei Liu and Jinfeng Sun and Jinnan Long and Lichao Sun
and Chang Liu and Xiaofan Wang and Long Zhang and
Pengyuan Hao and Zhongkai Wang and Yanting Cui and
Renjie Wang and Yuquan Li",
title = "Assessing the Interactive Effects of High Salinity and
Stocking Density on the Growth and Stress Physiology of
the \geoname{Pacific} White Shrimp \bioname{Litopenaeus
vannamei}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "62",
day = "31",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020062",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/62",
abstract = "This study was conducted to evaluate the effects of
high salinity combined with stocking density on
Litopenaeus vannamei. Three salinity gradients, namely,
28 g/L, 36 g/L, and 44 g/L, and two stocking densities,
namely, 300 and 600 shrimp/m 3, were used to analyze
the synergistic effect of high salinity and stocking
density on the growth performance, digestibility, and
energy budgets of L. vannamei. The experimental testing
period lasted 45 days. The research results showed that
a salinity level of 36 g/L was the most suitable
salinity level for shrimp growth under both high and
low stocking densities. The body weight, specific
growth rate, and relative weight gain of the shrimp in
the 36 g/L salinity group were significantly higher
than those in the other two salinity groups under both
high and low stocking densities. The high-density
farming group with 600 shrimp/m 3 exhibited a
significant inhibition of shrimp growth compared to the
low-density group under the same salinity conditions.
The activities of amylase, lipase, and protease in the
high-density-group shrimp gradually decreased with an
increase in salinity, and the three digestive enzymes
had the same overall effect of changing trends. This
indicates that under high-density farming conditions,
the increase in salinity is not conducive to the
digestive function of shrimps. At the same time, the
proportion of respiratory energy to feeding energy
gradually decreased in the high-density group and with
the increase in salinity. However, under the same
salinity conditions, the higher the stocking density,
the higher the energy consumed by respiration compared
to the low-density group. In addition, the expression
of the growth-related gene's small nuclear
ribonucleoprotein polypeptide G (SNRPG) under high
stocking density was significantly lower than that in
the low-density group at a salinity of 28 g/L, and
ribosomal protein L7 (RPL7) expression was also
significantly lower under high stocking density than
that in the low-density group at a salinity of 44 g/L.
The expression levels of molting-related genes retinoid
X receptor (RXR), ecdysone receptor (ECR), and
ecdysone-induced protein 75 (E75) were significantly
higher in the 36 g/L salinity group compared with the
other two salinity groups under high-stocking-density
treatment. The findings indicate that the synergistic
effects of salinity and stocking density have a
significant impact on the growth of L. vannamei, and
excessive salinity would inhibit its growth in the
process of high-density culturing.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sanz-Gonzalez:2024:SOA,
author = "Juan Carlos Sanz-Gonz{\'a}lez and Amalia Jurado-Mc
Allister and Mercedes Navarro-Mart{\'\i}nez and Rosa
Mart{\'\i}nez {\'A}lvarez-Castellanos and Ivan
Felis-Enguix and Yassine Yazid and Yahya El-Mansouri
and Fernando {De Miquel-Moral} and Hamid Errachdi and
Ana Juan-Lici{\'a}n",
title = "Sensing Offshore Aquaculture Infrastructures for
Data-Driven Dynamic Stress Analysis",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "61",
day = "31",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020061",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/61",
abstract = "The presence of escaped fish in aquaculture facilities
as a result of harsh meteorological conditions (more
pressing in the face of climate change) requires a
better understanding of this dynamic behaviour through
vigilant monitoring and validated numerical models. In
this context, data from strain and stress sensors as
well as meteorological and current sensors installed at
an aquaculture farm in the Region of Murcia (Spain)
were collected, processed and analysed. Among them, the
first results on the relationship between load and
current sensors are presented. Due to the complexity of
the time series, various analyses were conducted to
examine their interrelation, encompassing the
regression analysis of raw data and data segmented into
different time intervals. Through this analysis, it was
observed that employing distinct time windows better
elucidated the data variability. Furthermore, an
optimal data window of 240 data points was identified,
demonstrating a significantly improved explanatory
power, with the coefficient of determination (R 2)
increasing by approximately 0.8 depending on the
section. This paves the way for optimising the
monitoring features that must be carried out to relate
cause-and-effect variables in the behaviour of these
offshore infrastructures.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sun:2024:RUC,
author = "Yueping Sun and Bikang Yuan and Ziqiang Li and Yong
Liu and Dean Zhao",
title = "Rethinking Underwater Crab Detection via Defogging and
Channel Compensation",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "60",
day = "30",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020060",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/60",
abstract = "Crab aquaculture is an important component of the
freshwater aquaculture industry in China, encompassing
an expansive farming area of over 6000 km 2 nationwide.
Currently, crab farmers rely on manually monitored
feeding platforms to count the number and assess the
distribution of crabs in the pond. However, this method
is inefficient and lacks automation. To address the
problem of efficient and rapid detection of crabs via
automated systems based on machine vision in
low-brightness underwater environments, a two-step
color correction and improved dark channel prior
underwater image processing approach for crab detection
is proposed in this paper. Firstly, the parameters of
the dark channel prior are optimized with guided
filtering and quadtrees to solve the problems of
blurred underwater images and artificial lighting.
Then, the gray world assumption, the perfect reflection
assumption, and a strong channel to compensate for the
weak channel are applied to improve the pixels of red
and blue channels, correct the color of the defogged
image, optimize the visual effect of the image, and
enrich the image information. Finally, ShuffleNetV2 is
applied to optimize the target detection model to
improve the model detection speed and real-time
performance. The experimental results show that the
proposed method has a detection rate of 90.78\% and an
average confidence level of 0.75. Compared with the
improved YOLOv5s detection results of the original
image, the detection rate of the proposed method is
increased by 21.41\%, and the average confidence level
is increased by 47.06\%, which meets a good standard.
This approach could effectively build an underwater
crab distribution map and provide scientific guidance
for crab farming.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jung:2024:TID,
author = "Hyeok-Chan Jung and Jun-Hwan Kim and Ju-Chan Kang",
title = "Toxic Impact of Dietary Cadmium on Bioaccumulation,
Growth, Hematological Parameters, Plasma Components,
and Antioxidant Responses in Starry Flounder
(\bioname{Platichthys stellatus})",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "59",
day = "30",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020059",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/59",
abstract = "Starry flounders (Platichthys stellatus) (average
weight---114.69 {\pm} 13 g, length---19.0 {\pm} 0.21
cm) underwent a 4-week exposure to varying dietary
cadmium (Cd) concentrations: 0, 10, 20, 40, 60, and 80
mg/kg. Bioaccumulation patterns were revealed in the
intestine, kidney, liver, and gills in descending order
of accumulation. Significant declines in growth
indicators---body weight gain (BWG), specific growth
rate (SGR), and feed efficiency ratio percentage
(FER)---were evident at 80 mg Cd/kg for 2 weeks and
beyond 60 mg Cd/kg for 4 weeks. Hematological
parameters, including red blood cell (RBC) count,
hematocrit (Ht), and hemoglobin (Hb), remarkably
reduced at 80 mg Cd/kg for both 2 and 4 weeks. The
total plasma protein reduced significantly after
exposure to Cd for 2 and 4 weeks, alongside increased
glucose levels, glutamic oxaloacetic transaminase
(GOT), and glutamic pyruvate transaminase (GPT).
Considerable increases in antioxidant
responses---superoxide dismutase (SOD), catalase (CAT),
glutathione S-transferase (GST), and glutathione (GSH)
levels---were observed after exposure to Cd for 2 and 4
weeks. P. stellatus exhibits a high accumulation of
dietary Cd in specific tissues. Moreover,
concentrations above 60 mg Cd/kg adversely affected the
growth performance, hematological parameters, plasma
components, and antioxidant responses.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vuorinen:2024:TDM,
author = "Pekka J. Vuorinen and Reijo K{\"a}kel{\"a} and Tapani
Pakarinen and Petri Heinimaa and Tiina Ritvanen and
Soili Nikonen and Mervi Rokka and Marja Kein{\"a}nen",
title = "Thiamine Deficiency {M74} Developed in Salmon
(\bioname{Salmo salar}) Stocks in Two \geoname{Baltic
Sea} Areas after the Hatching of Large Year-Classes of
Two Clupeid Species --- Detected by Fatty Acid
Signature Analysis",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "58",
day = "30",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020058",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/58",
abstract = "Lipid-related thiamine (vitamin B1) deficiency of
Baltic salmon (Salmo salar), the M74 syndrome, is
generally caused by feeding on abundant young sprat
(Sprattus sprattus) in the Baltic Proper, the main
foraging area of these salmon. In 2014, a strong
year-class of sprat was hatched in the Baltic Proper,
and a strong herring (Clupea harengus) year-class was
hatched in the Gulf of Bothnia, where herring is the
dominant salmon prey. The fatty acid (FA) signatures of
prey fish in muscle or eggs of second sea-year spawners
suggested that 27\% of wild River Simojoki and 68\% of
reared River Dal salmon remained in the Gulf of Bothnia
in 2014 instead of continuing to the Baltic Proper. In
2016, 23\% of the M74 females of the River Simojoki and
58\% of the River Dal originated from the Gulf of
Bothnia, and 13\% and 16\%, respectively, originated
from the Baltic Proper. Some salmon from the River
Neris in the southern Baltic Proper had also been
feeding in the Gulf of Bothnia. In general, low free
thiamine (THIAM) concentration in eggs was associated
with high lipid content and high docosahexaenoic acid
(DHA, 22:6 {n$-$3}) and {n$-$3} polyunsaturated FA
({n$-$3} PUFA) concentrations in muscle but not in
eggs. A higher THIAM concentration and lower
proportions of DHA and {n$-$3} PUFAs in Arctic Ocean
salmon eggs, despite higher egg lipid content,
indicated that their diet contained fewer fatty fish
than the Baltic salmon diet. Hence, M74 originated by
foraging heavily on young fatty sprat in the Baltic
Proper or herring in the Gulf of Bothnia.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:USD,
author = "Suyu Liu",
title = "Using Sustainable Development Goal Indicator 14.7.1 to
Measure Sustainable Fishery: The Statistical
Limitations",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "57",
day = "30",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020057",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/57",
abstract = "Sustainable Development Goal (SDG) Indicator 14.7.1 is
the only statistical indicator under the SDG framework
which measures the progress to achieve sustainable
fisheries. However, despite its advantages, it suffers
from substantial limitations. One main problem is that
it is ambiguous what this indicator really measures and
what the definition of sustainable fisheries is, which
is the core concept of SDG Indicator 14.7.1. In
particular, this indicator has both an environmental
dimension and an economic dimension, but it is unclear
how one indicator can exactly reflect these two
dimensions at the same time. In addition, while SDG
Indicator 14.7.1 emphasizes inclusiveness in name, its
definition, methodology, and practical application
reveal the exclusion of a substantial number of
countries from this indicator. These limitations not
only diminish the anticipated functionality of SDG
Indicator 14.7.1 but also contradict the objective of
inclusivity. Such shortcomings of SDG Indicator 14.7.1
are possibly a result of the non-statistical
considerations during the development of this
indicator, including possible political influence on
the statistical scientific process. A number of
implications for improving the further development and
use of this indicator are provided, such as the
provision of more specific data and metadata",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2024:SPY,
author = "Junru Hu and Lei Wang and Guoxia Wang and Hongxia Zhao
and Huijie Lu and Kai Peng and Wen Huang and Zhenxing
Liu and Ding Liu and Yuping Sun",
title = "Selenium Protects Yellow Catfish (\bioname{Tachysurus
fulvidraco}) from Low-Temperature Damage via the
Perspective Analysis of Metabolomics and Intestinal
Microbes",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "56",
day = "30",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020056",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/56",
abstract = "The effects of selenium supplementation in Tachysurus
fulvidraco (T. fulvidraco) on low-temperature stress
are not known. In this study, 280 healthy T. fulvidraco
were divided into two groups, the G0 group (a control
group) and the T0 group (a selenium treatment group on
a 0.22 mg/kg diet), for a 6-week feeding time. Then,
low-temperature stress (water temperature dropped from
26 to 13 {$^\circ $}C, with a rate of 1 {$^\circ $}C/h)
was administered after that. The feeding results showed
that selenium increased the percent weight gain (PWG),
specific growth rate (SGR), and survival rate (SR) of
T. fulvidraco and decreased the feed conversion rate
(FCR), but these differences were not significant (p
{$>$} 0.05). Under low temperatures, selenium still has
no significant effects on antioxidant indexes such as
glutathione peroxidase (GSH-Px) activity, superoxide
dismutase (SOD) activity, and malondialdehyde (MDA)
content in serum (p {$>$} 0.05). However, metabolomic
analysis revealed that selenium caused changes in
lipids and lipid-like molles, organic acids and their
derivatives, and fatty acyls. Choline, linoleic acid,
and glycerophospholipid metabolism pathways; d-arginine
and d-ornithine metabolism; valine, leucine, and
isoleucine degradation; and biosynthesis pathways, as
well as pyrimidine metabolism pathways, were activated
to produce these metabolites to combat against this
stress. In addition, selenium increased the diversity
of intestinal microbes in T. fulvidraco and decreased
the relative abundance of Plesiomonas. However, the
combined analysis showed the intestinal microbe changes
did not affect metabolite production. In summary,
selenium activated lipid, carbohydrate, and amino acid
metabolism for energy substance provision, reduced the
oxidation and production of other harmful substances,
and increased the intestinal microbe diversity of T.
fulvidraco to improve resistance to low-temperature
stress.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Torres:2024:PPE,
author = "Susana Torres and Montserrat Compa and Antonio Box and
Samuel Pinya and Antoni Sureda",
title = "Presence and Potential Effects of Microplastics in the
Digestive Tract of Two Small Species of Shark from the
\geoname{Balearic Islands}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "55",
day = "29",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020055",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/55",
abstract = "In recent years, there has been an increase in the
reporting of plastic pollution in the marine
environment and its effects on marine animals,
especially bony fish. However, the prevalence and
effect, particularly concerning biomarkers of oxidative
stress, in elasmobranchs remain underreported and
unknown. In this study, microplastics were observed in
two elasmobranch species from the Balearic Islands: the
small-spotted catshark (Scyliorhinus canicula) with an
average of 4.38 {\pm} 1.77 items per individual, and
the blackmouth catshark (Galeus melastomus) with an
average of 8.31 {\pm} 2.46 items per individual.
Moreover, for the first time, antioxidant and
pro-inflammatory responses were determined in the
digestive tract of individuals of both species.
Specifically, the activation of antioxidant defences,
mainly superoxide dismutase activity and glutathione
levels, was observed, while the pro-inflammatory marker
myeloperoxidase activity was also greater in
individuals with a high abundance of microplastic
items. Additionally, a significant increase in
glutathione S-transferase activity in catsharks with
high microplastic ingestion was evidenced, suggesting
that the detoxification process was activated. Overall,
the results of this study highlight that both catshark
species are ingesting microplastics, which, in turn,
are causing physiological effects at a cellular level.
Considering this, continued monitoring of these species
should include the presence of microplastics, and the
results from this study can serve as baseline data for
future research.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Janampa-Sarmiento:2024:FRV,
author = "Peter C. Janampa-Sarmiento and Francisco Y. T. Reis
and Renata C. Egger and Santiago B. de P{\'a}dua and
S{\'o}stenes A. C. Marcelino and Jo{\~a}o L. R. Cunha
and Felipe Pierezan and Henrique C. P. Figueiredo and
Guilherme C. Tavares",
title = "First Report of \bioname{Vibrio vulnificus} Outbreak
in Farm-Raised Sorubim (\bioname{Pseudoplatystoma} sp.)
from \geoname{Brazil}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "54",
day = "29",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020054",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/54",
abstract = "Vibrio vulnificus is an opportunistic pathogen in
humans and exhibits pathogenic behavior in several
aquaculture fish species. To date, in Brazil, there are
no reports of V. vulnificus outbreaks in farmed fish.
However, in 2019, sorubim (Pseudoplatystoma sp.) in the
grow-out phase with clinical signs and cumulative
mortality of 40\% was registered. We aimed to identify
and characterize the etiological agents of this
outbreak. Seven moribund fish were sampled for
bacteriological studies, and isolates were obtained
from the brain and kidneys. Bacterial identification
using matrix-assisted laser desorption ionization mass
spectrometry (MALDI-TOF/MS) revealed V. vulnificus. One
isolate, RP4, was used for identification via dnaJ and
16S rRNA gene sequencing and antimicrobial
susceptibility evaluation. Sorubim juveniles were
experimentally challenged with RP4 isolate via
intracelomic injection (IC, 10 7 colony-forming units
[CFU] fish {$-$1}) and immersion bath (IB, 10 6 CFU mL
{$-$1} for 30 min). Identities to V. vulnificus of dnaJ
and 16S rRNA genes by BLAST analysis were higher than
92\% and 98\%, respectively. Susceptibility to
oxytetracycline, florfenicol, and other antimicrobial
molecules was also observed. In the IC-challenged
group, the mortality rate was 100\% with V. vulnificus
recovered from fish organs (brain, liver, spleen, and
kidney). Additionally, splenic endothelium alterations
were observed in the IC group. On the contrary, the
control and IB groups did not develop any clinical
signs, mortality, or bacterial recovery after 7 days of
challenge. To the best of our knowledge, this is the
first report of pathogenic V. vulnificus in farmed
Pseudoplatystoma sp.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Linh:2024:BSF,
author = "Nguyen Vu Linh and Supreya Wannavijit and Khambou
Tayyamath and Nguyen Dinh-Hung and Thitikorn
Nititanarapee and Md Afsar Ahmed Sumon and Orranee
Srinual and Patima Permpoonpattana and Hien Van Doan
and Christopher L. Brown",
title = "Black Soldier Fly (\bioname{Hermetia illucens}) Larvae
Meal: a Sustainable Alternative to Fish Meal Proven to
Promote Growth and Immunity in Koi Carp
(\bioname{Cyprinus carpio {\rm var.} koi})",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "53",
day = "28",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020053",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/53",
abstract = "Insect meal has shown promise as a potentially
sustainable source of nutrients for aquafeeds, offering
an alternative to expensive and ecologically
undesirable ingredients, in the context of population
explosion and climate change. Despite this promising
outlook, its effects on fish growth and immune
responses remain to be thoroughly investigated. Our
scientific goal was to experimentally test responses to
replacements of the fish meal with a protein source
derived from black soldier fly larvae meal (BSFLM).
Possible impacts on growth, immunological response, and
the expression of selected immune-system related genes
were evaluated in Koi carp (Cyprinus carpio var. koi)
using a biofloc culture system. Three hundred fish
(20.0 {\pm} 0.2 g) were allocated into five groups: a
control group receiving a basal diet containing 0 g kg
{$-$1} BSFLM and four experimental groups in which fish
meal was replaced with 50, 100, 150, and 200 g kg
{$-$1} BSFLM for eight weeks. After 4 weeks of feeding,
there were no statistically significant differences in
specific growth rate (SGR), feed conversion ratio
(FCR), and survival rate between fish fed
BSFLM-enriched diets at 50, 100, 150 g kg {$-$1} and a
control (0 g kg {$-$1} BSFLM) diet. However, fish fed
200 g kg {$-$1} BSFLM showed significantly improved
weight gain (WG) and SGR compared to the control after
4 weeks; this difference persisted through 8 weeks (p
{$<$} 0.05). After eight weeks, there was a moderate to
weak negative linear regression shown in FCR (r =
0.470) and SR (r = 0.384), respectively, with the BSFLM
levels, but significant and highly correlated linear
relationships were observed in WG (r = 0.917) and SGR
(r = 0.912). Immunological response analysis showed
slight changes in lysozyme and peroxidase levels by
replacing fish meal with BSFLM, but these apparent
differences were not significantly related to
experimental diets. Interestingly, mRNA transcripts of
immune-related genes (TNF-{\alpha}, TGF-{\beta}, IL1,
IL10, and hsp70) were upregulated in the groups
receiving higher amounts of BSFLM, with statistically
significant differences observed in certain
comparisons. Our findings reveal that fish meal can be
effectively replaced by BSFLM, and that this not only
has a positive effect on immune-related gene expression
in Koi carp, but also on growth rate, pointing to the
future potential role of BSFLM as an alternative fish
meal protein in aquafeed formulation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2024:RAS,
author = "Ao Chen and Zehua Lv and Junbo Zhang and Gangyi Yu and
Rong Wan",
title = "Review of the Accuracy of Satellite Remote Sensing
Techniques in Identifying Coastal Aquaculture
Facilities",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "52",
day = "27",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020052",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/52",
abstract = "The predominant form of aquaculture is the facility
fishery, which is also subject to significant impacts
from marine disasters. Conducting research on the
extraction of facility fishery areas based on remote
sensing technology is crucial to efficiently
comprehending the configuration of coastal culture
patterns and to establishing scientifically sound plans
for managing and administering these areas. The
extensive dispersion of facility fishery areas in
coastal regions poses a challenge to the conduction of
comprehensive field surveys. The utilization of
satellite remote sensing images for information
extraction has emerged as a significant area of
research in the fields of coastal fishery and
ecological environment. This study provides a
systematic description of the current research status
of coastal fishery area extraction methods using remote
sensing technology from 2000 to 2022 reported in the
literature. The methods discussed include the visual
interpretation method, image element-based
classification, object-based classification, supervised
classification, unsupervised classification, and neural
network classification. The extraction accuracy of each
method in the coastal facility fishery area is
evaluated, and the advantages and disadvantages of
these methods, as well as their limitations and
existing problems, are analyzed in detail, to construct
a reference framework for the investigation of the
high-precision extraction of facility fishery areas
from satellite remote sensing images.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Johnston:2024:RPG,
author = "Christina U. Johnston and Christopher J. Kennedy",
title = "A Review of {P}-Glycoprotein Function and Regulation
in Fish",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "51",
day = "27",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020051",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/51",
abstract = "The teleost ATP Binding Cassette (ABC) transporter
P-glycoprotein (P-gp) is an active transmembrane
transporter that plays a pivotal role in facilitating
the movement of both endogenous and xenobiotic
substrates (moderately hydrophobic and amphipathic
compounds) across cell membranes. P-gp exhibits
substrate specificity often shared with other ABC
transporters and solute carrier proteins, thereby
ensuring the maintenance of chemical homeostasis within
cells. These transporters are integral to chemical
defense systems in fish, as they actively expel a wide
range of substrates, primarily unmodified compounds,
from cells. This transport process assists in
preventing chemical absorption (e.g., intestine),
safeguarding sensitive tissues (e.g., brain and
gonads), and effectively excreting substances (e.g.,
liver and kidney). Upregulated P-gp export activity in
aquatic animals results in the multi-xenobiotic
resistance (MXR) phenotype that plays an essential
protective role in survival in contaminated
environments. Pollutants inhibiting P-gp are termed
chemosensitizers and heighten fish sensitivity to toxic
P-gp substrates. While the known intrinsic functions of
P-gp in fish encompass steroid hormone and bile acid
processing, relatively little attention has been given
to endogenous substrates and inhibitors. Fish
P-glycoprotein regulation is orchestrated by pivotal
nuclear transcription factors, including pregnane X
receptor (PXR) and nuclear factor erythroid 2-related
factor 2 (Nrf2). This comprehensive review provides
profound insights into P-gp's significance across
diverse fish species, contributing to an enhanced
understanding of fish physiology, evolution, and
toxicology, and provides information with potential
applications, such as environmental monitoring.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jungwirth:2024:ECA,
author = "Arne Jungwirth and Anna Horsfield and Paul
N{\"u}hrenberg and Stefan Fischer",
title = "Estimating Cognitive Ability in the Wild: Validation
of a Detour Test Paradigm Using a Cichlid Fish
(Neolamprologus pulcher)",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "50",
day = "27",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020050",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/50",
abstract = "Cognitive abilities vary within and among species, and
several hypotheses have been proposed to explain this
variation. Two of the most prominent hypotheses
regarding the evolution of cognition link increased
social and habitat complexity with advanced cognitive
abilities. Several studies have tested predictions
derived from these two hypotheses, but these were
rarely conducted under natural conditions with wild
animals. However, this is of particular importance if
we aim to link cognitive abilities with
fitness-relevant factors to better understand the
evolution of cognition. The biggest hurdle to assessing
cognition in the wild is to find a suitable setup that
is easy to use under field conditions. Here, we set out
to evaluate an extremely simple test of cognitive
ability for use with a broad range of aquatic animals
in their natural habitat. We did so by developing a
detour test paradigm in which fish had to detour a
clear obstacle to reach a food reward. By altering the
difficulty of the task, we confirmed that this setup is
a valid test of cognitive abilities in wild groups of a
Lake Tanganyika cichlid, Neolamprologus pulcher.
Subsequently, we probed specific predictions from the
two major hypotheses regarding cognitive evolution
using the most difficult test configuration.
Specifically, we tested the variation in cognitive
abilities among groups of different sizes occupying
habitats of varying complexity. We find mixed support
for both hypotheses, but we hope that our work inspires
future investigations on the evolution of cognition in
Lake Tanganyika cichlids.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ma:2024:VCG,
author = "Xueyan Ma and Wu Jin and Guohua Lv and Wanwen Chen and
Dongpo Xu and Pao Xu and Dan Hua and Haibo Wen",
title = "In Vitro Culture of Glochidia and Morphological
Changes in Juveniles of the Endangered Freshwater
Mussel \bioname{Solenaia oleivora}",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "49",
day = "27",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020049",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/49",
abstract = "The artificial propagation of the endangered Solenaia
oleivora, with unknown fish hosts, was performed via in
vitro culture with bighead carp, grass carp, common
carp, bovine, and rabbit sera. The effects of
glochidium density on transformation rates were
evaluated, and the development of juveniles that
metamorphosed successfully was documented. The control
group had a transformation rate of 0 and a
contamination rate of 0. No significant differences
were found in the transformation and contamination
rates of the bighead carp, grass carp, and common carp
serum groups, and their transformation rates were
significantly higher, and contamination rates were
significantly lower than those of the bovine and rabbit
serum groups. Moreover, no significant differences were
observed in the transformation rates of glochidia
(culture density, 2000--5000 glochidia/dish) in
contamination-free conditions. Specimen shell
length/height increased from 1.08 {\pm} 0.01 on the
first day to 3.08 {\pm} 0.29 during the 13th week. On
the basis of anterior and posterior growth differences,
juvenile growth was divided into the following three
phases: the rapid anterior growth period, distinct
anterior and posterior idiophase, and rapid posterior
growth period. This study not only provides
technological support for the artificial propagation of
S. oleivora but also lays a foundation for resource
recovery.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yazici:2024:ARM,
author = "Metin Yazici and Fatemeh Zavvar and Seyed Hossein
Hoseinifar and Shiva Nedaei and Hien Van Doan",
title = "Administration of Red Macroalgae (\bioname{Galaxaura
oblongata}) in the Diet of the Rainbow Trout
(\bioname{Oncorhynchus mykiss}) Improved Immunity and
Hepatic Gene Expression",
journal = j-FISHES,
volume = "9",
number = "2",
pages = "48",
day = "26",
month = jan,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9020048",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:35 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/2/48",
abstract = "This study is designed to evaluate the effects of
dietary red macroalgae (Galaxaura oblongata) on growth
performance, serum, and skin mucus immunological and
antioxidant responses in rainbow trout (Oncorhynchus
mykiss). For this, rainbow trout were fed diets
containing different levels of G. oblongata (0 (ctrl),
0.5 (G1), and 1 (G2) \%) for 8 weeks. Following the
feeding trial, there were no significant differences in
growth performance between the experimental treatments
(p {$>$} 0.05). Total immunoglobulin (Ig) content and
lysozyme (LYZ) activity in serum were increased in fish
fed G. oblongata (p {$<$} 0.05), with the highest value
at (0.5\%). Regardless of the inclusion level, mucus
total Ig levels were significantly increased in the G.
oblongata groups (p {$<$} 0.05), and mucus LYZ activity
was not changed (p {$>$} 0.05). All groups fed G.
oblongata showed higher serum catalase (CAT),
superoxide dismutase (SOD), and glutathione peroxidase
(GPx) activities than the control group (p {$<$} 0.05).
However, skin mucus SOD activity increased more in the
group fed 1\% of G. oblongata than the other groups (p
{$<$} 0.05). Additionally, the skin mucus GPx activity
showed higher values in the group fed 0.5 and 1\% G.
oblongata than in the control (p {$<$} 0.05). No
significant differences were recognized between the
experimental treatments in terms of CAT activity and
malondialdehyde (MDA) concentration (p {$>$} 0.05). G.
oblongata up-regulated gpx gene expression with the
maximum value at the group fed 1\% G. oblongata (p
{$<$} 0.05). Additionally, interleukin 6 (il-6) and
tumor necrosis factor-alpha (tnf-{\alpha}) gene
expressions were significantly up-regulated in fish fed
1\% compared with the control and 0.5\% groups. Based
on the results, 0.5--1\% G. oblongata can be used in
the fish diet and enhance immunity without causing
impairment in growth.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Brown:2024:ATE,
author = "Pete Brown and Tomas Araya-Schmidt and Terry Bungay
and Paul D. Winger",
title = "Assessing the Technical and Economic Viability of
Galvanizing Snow Crab (\bioname{Chionoecetes opilio})
Traps",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "109",
day = "19",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030109",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/109",
abstract = "Commercial harvesting of snow crabs (Chionoecetes
opilio) began in Newfoundland and Labrador, Canada, in
1967. Today, the fishery consists of 2188 active
fishing licenses and has grown into the province's most
economically valuable fishery. Snow crabs are captured
using conical traps consisting of a mild carbon steel
frame, hard plastic entry funnel and a jacket of
polyethylene netting. The frames of these traps corrode
over time, which is expedited by being deployed in
marine environments and stored on land near the ocean
when not in use. As a result, there is interest within
the community to increase the longevity of crab traps.
One solution is to galvanize the steel frames prior to
installing the funnel and netting. However, before
harvesters transition to galvanized traps, two
questions must be answered. Will the use of galvanized
steel negatively impact catch rates? Will the life
cycle of a crab trap be extended sufficiently to
justify the additional cost of galvanizing? This study
employed a generalized linear mixed model to evaluate
the catch of legal-sized male crabs (CPUE) during the
commercial fishery as a function of three trap frame
treatments (old traditional, new traditional and new
galvanized). We also assessed the economic viability of
galvanizing trap frames by evaluating the life cycle
cost (LCC) of traditional and galvanized traps to the
harvester. The LCC was calculated over a range of
inflation (0--6\%) and discount (3--20\%) rates. Our
results found no significant difference in CPUE between
new traps (traditional vs. galvanized) and concluded
that except during instances of very high discount
rates (12.9--19.9\%), it is economically favourable to
galvanize crab trap frames.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hoffling:2024:ASM,
author = "Fl{\'a}via Bander{\'o} Hoffling and Alex Silva
Marquezi and Isabela Pinheiro and Cedric Simon and
Artur Nishioka Rombenso and Walter Quadros Seiffert and
Felipe do Nascimento Vieira and Delano Dias Schleder",
title = "\bioname{Aurantiochytrium} sp. Meal as Feed Additive
for \geoname{Pacific} White Shrimp Reared under Low
Temperature and Challenged by {WSSV} in Association
with Thermal Stress",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "108",
day = "18",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030108",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/108",
abstract = "A study was conducted to test the inclusion of
Aurantiochytrium sp. meal in the rearing of Penaeus
vannamei grown in a clear water system and at a
suboptimal temperature of 22 {$^\circ $}C. The doses
tested were 0 (control), 1, 2, 3, and 4\% at a stocking
density of 100 shrimp/m 3. Rearing was carried out with
aeration and individual heaters, and seawater
temperature was controlled with a chiller. After nine
weeks, shrimp were weighed and hemolymph was collected
for hemato-immunological tests, and growth performance
were calculated. Shrimp raised at 22 {$^\circ $}C and
fed a 1\% of dietary supplementation of
Aurantiochytrium sp. meal were superior in
immunological parameters. After the dietary assay, a
total of 42 shrimps (10.9 {\pm} 0.06 g) per treatment
were infected with White Spot Syndrome Virus (WSSV).
They were orally infected with 2.6 {\pm} 10 6 virus
particles per g of the animal, maintained at a
suboptimal temperature of 22 {$^\circ $}C for 108 h,
and acclimated to an optimal temperature of 28 {$^\circ
$}C for an additional 48 h. At 7 days post infection,
surviving shrimp were collected for
hemato-immunological analysis. Cumulative mortality
results showed that shrimp fed diets containing 3\% and
4\% Aurantiochytrium sp. meal had higher survival than
other treatments when challenged with WSSV.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yu:2024:HSS,
author = "Jun Yu and Lihong Wen and Siyuan Liu and Heng Zhang
and Zhou Fang",
title = "Habitat Suitability of the Squid
\bioname{Sthenoteuthis oualaniensis} in
\geoname{Northern Indian Ocean} Based on Different
Weights",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "107",
day = "15",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030107",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/107",
abstract = "Data from the fishery of S. oualaniensis in the
northern Indian Ocean from January to March and October
to December 2017 to 2019 were modeled with sea surface
temperature (SST), wind speed (WS), and
photosynthetically active radiation (PAR). In this
study, the fishing effort was used to evaluate the
suitability index (SI) at SST, WS, and PAR. An
integrated habitat suitability model (HSI) was
developed with different weighting scenarios and
weighting schemes. The optimal case was selected by
calculation and comparison with the proportion of
catch, effort, and catch per unit effort (CPUE) in the
HSI interval (0~0.2, 0.2~0.6, 0.6~1); validation was
performed using data from 2019. The weight of the
optimal HSI model was 0.25 for sea surface temperature
and photosynthetically active radiation, and 0.5 for
wind speed. This model yielded the best performance and
could accurately predict the fishing ground of S.
oualaniensis in the northern Indian Ocean. The findings
suggest that the integrated HSI model can predict the
distribution of S. oualaniensis commendably, with wind
speed as the most important factor affecting the
spatial distribution of S. oualaniensis' habitat in the
northern Indian Ocean. By analyzing habitat selection
by S. oualaniensis, this study verified and predicted
the distribution of squid in the northern Indian Ocean,
which allows the distribution of squid resources and
fishing grounds to be modeled, and for the sustainable
use of squid fishery resources.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rodriguez-Dominguez:2024:FAD,
author = "Guillermo Rodr{\'\i}guez-Dom{\'\i}nguez and Eugenio
Alberto Arag{\'o}n-Noriega and Jorge Pay{\'a}n-Alejo
and Jaime Edzael Mendivil-Mendoza and Marcelo Vidal
Curiel-Bernal and Wenceslao Valenzuela-Qui{\~n}onez and
Ricardo Ur{\'\i}as-Sotomayor",
title = "The Fractal Approach to Describe Growth of Farmed
Marine Species: Using Double and Triple Logistic
Models",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "106",
day = "12",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030106",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/106",
abstract = "Modeling individual growth in marine species for
aquaculture encounters many difficulties when the
species pauses its growth but resumes its later after
the disrupting phenomenon (environmental or
physiological) has been overcome. Seasonal or
oscillatory growth has been addressed by modifying
existing models, such as von Bertalanffy and Gompertz,
to include an oscillatory component in this study. The
novelty of this study lies in the fractal approach used
to analyze growth using multiple logistic functions.
Three commercially farmed marine species were studied,
including shellfish, crustacea, and finfish. The
oscillatory version of the von Bertalanffy model as
well as double and triple logistic functions were used
for analysis. The best model was selected using the
information theory, Specifically the Akaike criterion
(AIC) and the Bayesian criterion (BIC). Normal and
log-normal distributions of error were assumed. The
triple logistic model with log-normal distribution in
the error structure was found to be the best model to
describe the growth pattern of the three commercially
farmed species as it obtained the lowest AIC. Overall,
this study concludes that the fractal approach is the
most effective way to describe growth in farmed
species, including shellfish, crustacean, and
finfish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2024:HEG,
author = "Wencheng Xu and Yanzhe Wang and Guodong Wang and Lili
Zhang and Guiling Zhang and Zhipeng Huo and Hui Ge",
title = "Heritability Estimates for Growth Traits and
Correlation Analysis between Weight and Metamorphosis
Rate in the Bullfrog \bioname{Rana (Aquarana)
catesbeiana}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "105",
day = "09",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030105",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/105",
abstract = "Metamorphosis is a crucial process in the life cycle
of Rana (Aquarana) catesbeiana. R. catesbeiana
tadpoles, in their short larval period, possess a high
survival rate and also a highly competitive ability in
the amphibious stage. In actual seed production, the
economic traits of larval period and metamorphosis rate
are used as quantifiable indicators of quality for
individuals and populations, respectively. However,
studies of these economic traits in larval cultivation
and production are still lacking. In this study, we
constructed 40 full-sib families of R. catesbeiana and
measured the weight and metamorphosis rate of tadpoles
at different developmental stages. Subsequently, we
calculated the phenotypic and genetic association
between weight and metamorphosis rate in tadpoles and
assessed the heritability of these two traits. The
heritabilities of weight at three developmental stages
were all higher than 0.40 and decreased with
advancement of the developmental stage; the
heritability of the metamorphosis rate was 0.18 {\pm}
0.20, a moderate level. Correlation analysis of weight
and metamorphosis rate at each developmental stage in
each tadpole family showed that weight at stages 25-I,
25-II, and 25-III was significantly correlated at the
phenotypic level but non-significantly at the genetic
level. The metamorphosis rate was only moderately
associated with stage 25-III weight (0.38, p {$<$}
0.05). The results of this study confirm the importance
and transportability of tadpole weight in actual seed
production and provide basic data and a potentially
optimized direction for the selective breeding of
high-metamorphosis-rate bullfrogs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Christidis:2024:AOB,
author = "Georgios Christidis and Stratos Batziakas and
Panagiota Peristeraki and Evangelos Tzanatos and
Stylianos Somarakis and George Tserpes",
title = "Another One Bites the Net: Assessing the Economic
Impacts of \bioname{Lagocephalus sceleratus} on
Small-Scale Fisheries in \geoname{Greece}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "104",
day = "07",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030104",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/104",
abstract = "The assessment of the economic impacts of marine
invasive species is fundamental for adopting mitigation
measures, yet such impacts have been underreported in
the Mediterranean Sea. The silver-cheeked toadfish
(Lagocephalus sceleratus) is a toxic pufferfish that
since its introduction has seriously disturbed
small-scale fisheries along the eastern Mediterranean
coast. This species depredates on fishing gears,
causing damage to nets, longlines and commercial
catches. To quantify its economic impact on small-scale
fisheries, we interviewed 141 fishers from Crete
(Cretan and Libyan Sea) and the Ionian Sea (Greece)
during May 2020--December 2022. The mean annual
economic cost resulting from L. sceleratus depredation
was estimated at EUR 6315 {\pm} 2620 per vessel in
Crete and EUR 258 {\pm} 120 in the Ionian Sea.
Additionally, observer surveys carried out on board
small-scale fishing vessels in Crete showed that
depredation probability was significantly influenced by
fishing depth, sea surface temperature, gear type
(nets, longlines) and region (Cretan, Libyan Sea). L.
sceleratus was caught more frequently and in higher
numbers in the Libyan Sea. In response to depredation,
fishers in Crete have altered their fishing tactics in
terms of fishing in deeper waters, reducing fishing
time and changing the technical characteristics of
fishing gears. Our results underscore the adverse
impacts of L. sceleratus on Greek small-scale fishers,
emphasizing the need for region-specific management
plans where the species establishes large
populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jongjaraunsuk:2024:TLM,
author = "Roongparit Jongjaraunsuk and Wara Taparhudee and
Soranuth Sirisuay and Methee Kaewnern and Varunthat
Dulyapurk and Sommai Janekitkarn",
title = "Transfer Learning Model Application for
\bioname{Rastrelliger brachysoma} and \bioname{R.
kanagurta} Image Classification Using
Smartphone-Captured Images",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "103",
day = "07",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030103",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/103",
abstract = "Prior aquatic animal image classification research
focused on distinguishing external features in
controlled settings, utilizing either digital cameras
or webcams. Identifying visually similar species, like
Short mackerel (Rastrelliger brachysoma) and Indian
mackerel (Rastrelliger kanagurta), is challenging
without specialized knowledge. However, advancements in
computer technology have paved the way for leveraging
machine learning and deep learning systems to address
such challenges. In this study, transfer learning
techniques were employed, utilizing established
pre-trained models such as ResNet50, Xception,
InceptionV3, VGG19, VGG16, and MobileNetV3Small. These
models were applied to differentiate between the two
species using raw images captured by a smartphone under
uncontrolled conditions. The core architecture of the
pre-trained models remained unchanged, except for the
removal of the final fully connected layer. Instead, a
global average pooling layer and two dense layers were
appended at the end, comprising 1024 units and by a
single unit, respectively. To mitigate overfitting
concerns, early stopping was implemented. The results
revealed that, among the models assessed, the Xception
model exhibited the most promising predictive
performance. It achieved the highest average accuracy
levels of 0.849 and 0.754 during training and
validation, surpassing the other models. Furthermore,
fine-tuning the Xception model by extending the number
of epochs yielded more impressive outcomes. After 30
epochs of fine-tuning, the Xception model demonstrated
optimal performance, reaching an accuracy of 0.843 and
displaying a 11.508\% improvement in predictions
compared to the model without fine-tuning. These
findings highlight the efficacy of transfer learning,
particularly with the Xception model, in accurately
distinguishing visually similar aquatic species using
smartphone-captured images, even in uncontrolled
conditions.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Qiu:2024:ICC,
author = "Hengtong Qiu and Huan Wang and Xiaomin Yan and Lin Hu
and Yonglin Huang and Yanni Ye",
title = "The Identification of a Cell Cycle Regulation Gene
{{\em Cyclin E\/}} from \geoname{Hong Kong} Oysters
(\bioname{Crassostrea hongkongensis}) and Its Protein
Expression in Response to Salinity Stress",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "102",
day = "06",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030102",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/102",
abstract = "Hong Kong oysters (Crassostrea hongkongensis) are an
important marine bivalve with nutritional and
commercial value. The expanded off-bottom farming scale
in recent years makes the oysters more susceptible to
exposure to abiotic stresses, such as salinity stress,
an important environmental factor that has been proven
to have significant effects on oyster growth and
development. However, the molecular mechanism is still
unclear. Cyclin E is an important protein in the
process of cell cycle regulation that is indispensable
for propelling G1/S phase transition in a
dose-dependent manner. In order to investigate whether
the salinity stress affects cyclin E expression in
oysters, the cDNA sequence of C. hongkongensis~cyclin E
(Ch-CCNE) was isolated from a gill cDNA library, and
the 2.8 kbp length cDNA fragment contained a complete
open reading frame (ORF) encoding 440 amino acid
residues. Ch-CCNE mRNA was highly expressed in the
gonad and low in the adductor mussel, mantle, gill,
labial palp, and digestive gland. The recombinant CCNE
protein was expressed and purified in a pET32a(+)- CCNE
/ Escherichia coli BL21(DE3) system via IPTG induction
and was used for generating mice anti-Ch-CCNE
antiserums. Western blot analysis showed that the CCNE
protein in the gill was maintained at low expression
levels under either hypo- (5 ppt) or hyper- (35 ppt)
salinity, and could be produced at high levels under
appropriate salinity during a 10-day exposure period.
The immuno-localization indicated that the Ch-CCNE
protein was distributed in the nucleus. These results
suggested that either hypo- or hyper-salinity stress
could inhibit the CCNE expression of Hong Kong oysters
and their negative impact on cell division and
proliferation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Govindharajan:2024:ESM,
author = "Sattanathan Govindharajan and Balamuralikrishnan
Balasubramanian and Vivi Thapo and Sournamanikam
Venkatalakshmi and Wen-Chao Liu",
title = "Enhancement of Skin Mucus Immunity, Carotenoid
Content, Sexual Parameters, and Growth Response in
Guppy Fish (\bioname{Poecilia reticulata}) Fed with
Green Algae (\bioname{Chaetomorpha aerea}) Diets",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "101",
day = "06",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030101",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/101",
abstract = "The research aimed to analyze the influences of adding
marine green algae Chaetomorpha aerea to the diet of
guppy fish (Poecillia reticulate) on growth,
immunological responses in skin mucus, total carotenoid
content, and sexual characteristics. A total of 450
fish, with a mean body weight of 0.19 {\pm} 0.1 g and
30 fish per tank (triplicate), were randomly fed into
15 experimental tanks, each containing 50 L. Five
different diets with 0, 1, 2, 4, 8, and 10\% of C.
aerea g/kg diets were fed to P. reticulate for 30 days.
After 30 days, growth, immunological responses in skin
mucus, total carotenoid content, and sexual
characteristics were investigated. The results observed
that the feed conversion rate and fry output were
significantly (p {$>$} 0.05) decreased in experimental
groups compared to the control group. The results
revealed that the dietary inclusion of C. aerea algal
significantly increased (p {$<$} 0.05) in mucosal
immunological parameters containing lysozyme activity,
myeloperoxidase activity, total immunoglobulins, and
alternative complement activity, which were the highest
in the group with 4\% of C. aerea g/kg. Additionally,
lateral skin and the caudal fin of fish had higher
total carotenoid levels from the dietary C. aerea algae
diet than the control group, which were the highest in
the groups with 4\%. Among them, 4 and 8\% of C. aerea
g/kg diet resulted in better growth performance and
feed conversion ratio. Thus, the study suggested that
4\% of C. aerea g/kg diet has enrichment of immunity,
total carotenoid concentrations, and skin mucus
immunity of P. reticulate.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jiang:2024:TAR,
author = "Senhao Jiang and Yuting Lei and Ti Wang and Ruiting Ma
and Chunqiang Hou and Qiuning Liu",
title = "Transcriptome Analysis Revealed Potential
Immune-Related Genes of Head Kidney in the Yellow
Catfish (\bioname{Pelteobagrus fulvidraco}) Challenged
with \bioname{Aeromonas hydrophila}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "100",
day = "06",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030100",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/100",
abstract = "Yellow catfish (Pelteobagrus fulvidraco) is an
important freshwater fish species in aquaculture.
However, as intensive farming has rapidly expanded,
infectious diseases caused by various bacteria, such as
Aeromonas hydrophila, have also increased. We conducted
a transcriptomic analysis of head kidney from P.
fulvidraco stimulated by A. hydrophila strain SHOU. A
total of 43,249 unigenes with an average length of 1342
bp were obtained following assembly and annotation. By
analyzing GO and KEGG enrichment, many differentially
expressed genes (DEGs) and pathways related to immunity
were identified. Out of the 973 DEGs that were
identified, 553 were upregulated and 420 were
downregulated. Moreover, KEGG enrichment analysis
revealed that the innate immune system, including the
TNF signaling pathway, NF-kappa B signaling pathway,
and the Toll-like receptor signaling pathway involved
in the defense, is activated against infectious
response. Real-time quantitative reverse
transcription-PCR (qRT-PCR) analysis demonstrated that
immune response genes were upregulated in response to
A. hydrophila stimulation compared to the control. In
conclusion, this study provides valuable insights into
the immune defense mechanism of P. fulvidraco and sheds
light on the host immune genes involved in the response
to bacterial infection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Dube:2024:AAP,
author = "Edith Dube and Grace Emily Okuthe",
title = "Applications of Antimicrobial Photodynamic Therapy in
Aquaculture: Effect on Fish Pathogenic Bacteria",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "99",
day = "05",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030099",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/99",
abstract = "Increased infectious diseases and the reduced
effectiveness of antibiotics due to antimicrobial
resistance pose global challenges affecting the
aquaculture industry. As bacteria increasingly develop
antibiotic resistance, research scientists are shifting
their focus to technologies such as antimicrobial
photodynamic therapy (aPDT), which show potential for
treating and controlling fish infections without
promoting the development of resistant bacteria.
Various photosensitizers (PSs), both natural and
synthetic, are under investigation for their
application in aPDT within the aquaculture industry.
This shift is crucial for the sustainability of the
aquaculture industry, which plays a significant role in
achieving several of the United Nations (UN)
Sustainable Development Goals (SDGs). This review
highlights the application of aPDT against fish
pathogens in the industry and the types of PSs
utilized. It also explores the potential application of
this technique for treating and controlling fish
infections, along with the advantages and limitations
of its use in aquaculture production systems. Finally,
a conclusion and future perspectives are provided.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Eliso:2024:PMN,
author = "Maria Concetta Eliso and Barbara Bill{\`e} and Tiziana
Cappello and Maria Maisano",
title = "Polystyrene Micro- and Nanoplastics ({PS MNPs}): a
Review of Recent Advances in the Use of -Omics in {PS
NP} Toxicity Studies on Aquatic Organisms",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "98",
day = "05",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030098",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/98",
abstract = "In recent years, micro- and nanoplastics (MNPs) have
represented an emerging threat for the aquatic
environment due to their persistence and widespread
distribution. Indeed, their small size and increased
surface area lead to a high biological reactivity,
which can be crucial for the health status of biota. To
date, several studies have investigated lethal and
sublethal endpoints of MNPs, while one of the main
challenges is to understand their mechanisms of
toxicity in a comprehensive manner. With this aim, the
field of aquatic ecotoxicology can now benefit from the
-omics approaches. This review focuses on the recent
advances related to the use of transcriptomics,
proteomics, and metabolomics to deeply investigate the
molecular and biochemical responses of aquatic
organisms, both fishes and aquatic invertebrates, to
pristine polystyrene (PS) MNPs. The literature reviewed
revealed that transcriptomics and metabolomics are the
most frequently used -omic approaches. Overall, the
studies taken into consideration shed light on the
events triggered by PS MNPs at molecular and cellular
levels, identifying as mechanisms of toxicity the
pathways involved in oxidative stress, energy
metabolism, immune response, and the nervous system.
Future studies should therefore focus on the use and
integration of multi-omics approaches for a more
comprehensive understanding of the mechanisms involved
in MNPs toxicity.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2024:FCP,
author = "Xiandong Xu and Yanping Zhang and Liyun Ding and
Jiangfeng Huang and Zhiyong Zhou and Wenjing Chen",
title = "Farmed \geoname{Chinese} Perch (\bioname{Siniperca
chuatsi}) Coinfected with Parasites and Oomycete
Pathogens",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "97",
day = "04",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030097",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/97",
abstract = "Farming Chinese perch in aquaculture systems with
artificial diets is a new method and developing trend.
This method of raising Chinese perch has led to
outbreaks of new diseases. In 2022, a disease outbreak
occurred among farmed Chinese perch fed an artificial
diet in Jiangxi Province, China, during which 50\% of
the fish died. The clinical signs exhibited by the
diseased Chinese perch included decreased physical
vitality, anorexia, emaciation, and dorsal fin
ulceration with white cysts and flocs. Coinfection with
ciliate parasites and an oomycete was found. No
pathogenic bacteria were isolated from the microbial
test, and the viral detection results were negative.
After morphological and 18S or 28S rDNA sequence
clustering analysis, the parasites were identified as
Epistylis wuhanensis and Zoothamnium sp., while the
oomycete was identified as Achlya klebsiana. This
article discusses the interaction between parasitic and
oomycete coinfections in Chinese perch from the
perspective of the characteristics of Chinese perch
raised with an artificial diet. The relationships
between parasites, oomycete, and fish were also briefly
discussed. This is the first report of the coinfection
of Chinese perch with Epistylis wuhanensis, Zoothamnium
sp. and Achlya klebsiana.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2024:CSB,
author = "Yukai Yang and Heizhao Lin and Xiaolin Huang and
Hongbiao Dong and Yafei Duan",
title = "Changes in the Serum Biochemical Indices and
Intestinal Microbial Community of Rabbitfish
(\bioname{Siganus oramin}) in Three Different
Habitats",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "96",
day = "04",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030096",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/96",
abstract = "The rabbitfish Siganus oramin is an important
naturally caught and aquaculture fish species.
Intestinal microbiota can affect the metabolism and
immunity of fish, which is closely related to the
habitat of the host. In this study, we collected the
wild fry S. oramin from a natural sea area, and
cultured them in outdoor and indoor ponds,
respectively, and investigated the changes in serum
biochemical indexes and intestinal microbial community
in three different habitats. The results showed that
compared with the wild population, the serum total
protein content of the outdoor culture population
increased significantly. The indoor culture population
had significantly higher triglyceride and total
cholesterol contents than that of the outdoor culture
population. Additionally, the intestinal microbial
richness indexes ACE and Chao1 of the cultured
population were higher than those of the wild
population, especially the indoor culture, but Shannon
and Simpson had no obvious changes. The relative
abundances of Firmicutes, Spirochaetae and
Bacteroidetes increased in the outdoor culture
population, but decreased in the indoor culture
population; those of Proteobacteria and Cyanobacteria
were completely the opposite. Some putative beneficial
bacteria (Lactobacillus, Clostridium sensu stricto 1,
and Intestinibacter) and harmful bacteria (Vibrio,
Photobacterium, Desulfovibrio, Streptococcus) all
decreased in the intestines of the cultured population
when compared with the wild population, while
Akkermansia and Faecalibacterium were enriched in the
outdoor culture population. These results reveal that a
change in habitat environment, whether an outdoor or an
indoor pond, positively influenced the intestinal
microbiota of the rabbitfish, which is beneficial to
the healthy culture of the fish from the perspective of
microbial community.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lin:2024:CCR,
author = "Ainuo Lin and Yaxi Li and Zhi Yan and Huilin Zhang and
Baozhen Jiang and Jingjing Chen and Derui Wang and Huan
Wang and Xiaodong Li and Zhen Lu and Ke Li",
title = "Chemical Cues Released by Predators' Consumption of
Heterospecific Prey Alter the Embryogenesis of
Zebrafish",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "95",
day = "01",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030095",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/95",
abstract = "Environmentally cued hatching is prevalent, diverse,
and crucial to many animals' survival. Fish embryos use
a variety of chemical cues to initiate hatching to
avoid potential predators, yet the function of chemical
cues released from the predatory consumption of
heterospecific prey is largely unknown. Zebra cichlids
(Metriaclima estherae) are ferocious predators that can
feed on medaka (Oryzias latipes), though it is
impossible for this to occur in their natural habitat.
Zebrafish (Danio rerio) embryos have been employed as
experimental subjects due to their sensitivity to a
variety of chemical signals. In this study, zebrafish
embryos were subjected to three types of chemical
signals: predator cues (PCs, released from cichlids),
heterospecific cues (HCs, released from medaka), and
heterospecific dietary cues (HDCs, released from
cichlids that have ingested medaka). As a result, the
hatching times of zebrafish embryos were accelerated by
6.8\% and 12.6\% by PCs and HDCs, respectively. PCs and
HDCs cause significantly reduced morphology in
zebrafish embryos, including regarding total length,
eye length, dorsal fin length, trunk height, caudal fin
height, and body cavity, and increase yolk sac height.
The PCs and HDCs diminished the larvae's motion at 120
and 144 h post fertilization (hpf), which could be
attributed to non-developmental embryogenesis. Overall,
the impacts of HDCs on embryonic hatching,
developmental morphology, and locomotor were more
pronounced in comparison with PCs. Our findings
demonstrate that predators' dietary cues, even those
released after predation on heterospecific prey, can
modify embryogenesis, highlighting the critical
functions of chemical signals in predation risk
assessment using embryos.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Karimov:2024:LWA,
author = "Erkin Karimov and Bernhard Zeiringer and Johan Coeck
and Pieterjan Verhelst and Bakhtiyor Karimov and Otabek
Omonov and Martin Schletterer and Daniel S. Hayes",
title = "Length--Weight--Age Relationship of
\bioname{Schizothorax eurystomus} {Kessler}, 1872 and
Comparison to Other Snow Trout Species in
\geoname{Central Asia}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "94",
day = "29",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030094",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/94",
abstract = "This study presents a comprehensive analysis of the
length-weight relationship, condition factors, and age
of Schizothorax eurystomus in the Shakhimardan River
basin in Central Asia, along with a comparative
perspective to other Schizothorax species in the
region. The study found that S.~eurystomus exhibits
positive allometric growth, which is consistent with
similar patterns observed in this species from the Syr
Darya River basin. The two analyzed condition factors
showed mean values within the normal range, indicating
good feeding and environmental conditions. However,
significant disparities between minimum and maximum
values of these factors indicated varied growth
conditions which may be influenced by anthropogenic
factors. Age estimation using opercular bones showed
variations in the total length among fish of the same
age, and a clear age distribution pattern across
different sites. Younger fish predominantly inhabited
the shallower, warmer, and lower sections of the river,
which is impacted by agricultural water diversion,
while older specimens were found in areas with higher
discharge and deeper pools. Overall, this research
provides valuable insights into the life history traits
of S. eurystomus, underlining the need for sustainable
fishery management and conservation strategies in the
Shakhimardan River basin. The findings also emphasize
the importance of considering habitat quality and
anthropogenic pressures regarding understanding both
fish population dynamics and growth patterns.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mendes:2024:SHM,
author = "Hugo Mendes and Cristina Silva and Manuela Azevedo",
title = "Southern Horse Mackerel (\bioname{Trachurus
trachurus}) Spatio-Temporal Distribution Patterns Based
on Fine-Scale Resolution Data",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "93",
day = "29",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030093",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/93",
abstract = "In this study, the distribution patterns of southern
horse mackerel are examined using commercial fine-scale
resolution data. Using landings by size category and
VMS data from the Portuguese commercial bottom-trawl
fishery, which consistently targets horse mackerel,
this study provides a comprehensive analysis of horse
mackerel age distributions spanning a decade
(2010--2020). Importantly, this study addresses
potential biases in commercial effort data and
establishes the usefulness of commercial bottom-trawl
gear as a suitable method for sampling and evaluating
southern horse mackerel stock dynamics. Ordered
regression models were applied to allow for the
modelling of the distribution of multiple age
categories and investigate spatio-temporal migrations
off the Portuguese coast. Southern horse mackerel show
a widespread age distribution range and stable
abundance with indications of seasonal and spatial
patterns in the distribution of specific age groups.
The insights derived from this research contribute
valuable knowledge for understanding the dynamics and
distribution patterns of fish populations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pan:2024:CTH,
author = "Tingshuang Pan and Tong Li and Min Yang and He Jiang
and Jun Ling and Qian Gao",
title = "Cardiac Transcriptome and Histology of the Heart of
the Male \geoname{Chinese} Mitten Crab
(\bioname{Eriocheir sinensis}) under High-Temperature
Stress",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "92",
day = "28",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030092",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/92",
abstract = "High temperatures are important environmental
stressors affecting the metabolism, growth, immunity,
and mortality of Chinese mitten crabs (Eriocheir
sinensis). In this study, Chinese mitten crabs were
divided into two groups and exposed to temperatures of
35 {$^\circ $}C (thermal stress group) or 25 {$^\circ
$}C (control group) for 24 h, and the transcriptome of
the heart was analyzed. There were 4007 differentially
expressed genes (DEGs) between the thermal stress and
the control groups, including 2660 upregulated and 1347
downregulated genes. Heat shock proteins (HSPs) and
transcription factors (TFs) were temperature-sensitive
DEGs in Chinese mitten crabs. DEGs mainly focused on
protein processing in the endoplasmic reticulum,
ribosome biogenesis, glycine, serine, and threonine
metabolism, protein export, and insect hormone
biosynthesis pathways. A total of 28,916 SSRs and 59 TF
families, including 851 TFs, were detected among all
unigenes of E. sinensis transcripts. The qRT-PCR
results for the HSPs and apoptotic DEGs from the heart
exhibited the same trends as those in the E. sinensis
transcriptome data. Results of light microscopy
analyzing histological sections of the heart indicated
that most myocardial fibers were lysed, and the number
of nuclei and the connective tissue contents between
the myocardial layers were both reduced following 35
{$^\circ $}C exposure for 24 h.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wei:2024:MEM,
author = "Qingcong Wei and Dan Wang and Kaijin Wei and Bin Xu
and Jin Xu",
title = "The Mechanism of \bioname{Elizabethkingia miricola}
Infection of the Black Spotted Frog as Revealed by
Multi-Omics Analysis",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "91",
day = "28",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030091",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/91",
abstract = "Elizabethkingia miricola (E. miricola) is a
significant pathogen that causes the crooked head
disease in black spotted frogs. This disease has
plagued numerous frog farms in China and has resulted
in substantial losses to the frog farming industry.
Nonetheless, the exact mechanism that causes the
disease in frogs remains unknown. In this study,
transcriptomic and microbiomic analyses were conducted
to analyze frog samples infected with E. miricola to
reveal the infection mechanism of the pathogen. Liver
transcriptomic analysis indicated that the livers of
infected frogs had 1469 differentially expressed genes
when compared with an uninfected group. These DEGs are
mainly involved in immunity and metabolism, including
neutrophil extracellular trap formation, the NOD-like
receptor signaling pathway, leukocyte transendothelial
migration, chemokine signaling pathway, Fc gamma
R-mediated phagocytosis, and ``metabolism''-related
pathways such as the pentose phosphate pathway, carbon
metabolism, glycerophospholipid metabolism, and
glycerolipid metabolism. Similarly, 4737 DEGs were
found in the kidney of infected frogs. These DEGs are
mainly involved in immunity, including neutrophil
extracellular trap formation, the NOD-like receptor
signaling pathway, B cell receptor signaling pathway,
C-type lectin receptor signaling pathway, complement
and coagulation cascade, and Toll-like receptor
signaling pathway. Ten immune-associated DEGs were
screened in liver and kidney DEGs, respectively. And it
was hypothesized that E. miricola infection could
influence the host immune response. Microbiome analysis
results showed that some opportunistic pathogens such
as Citrobacter, Shigella, and Providencia were
significantly elevated (p {$<$} 0.05) in infected
frogs. Additionally, functional prediction confirmed
that most of the microbiota in infected frogs were
linked to metabolism-related KEGG pathways. In this
study, the screened genes linked to immunity showed an
association with the gut microbiome. The majority of
these genes were found to be linked with the abundance
of opportunistic pathogens. The results showed that E.
miricola infection led to the downregulation of immune
and metabolic-related genes, which led to the
inhibition of immune function and metabolic disorder,
and then increased the abundance of opportunistic
pathogens in the gut microbiota. The findings of this
study offer a preliminary foundation for comprehending
the pathogenic processes of E. miricola infection in
black spotted frogs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wei:2024:COD,
author = "Shuwei Wei and Zhong Hua and Yanping Yang and Fengjiao
Ma and Wei Han and Wei Zhang and Congping Ying and
Yanmin Deng and Kai Liu",
title = "Characterization of the Ovarian Development and
Associated Factors during the Breeding Migration of
\bioname{Coilia nasus} in the \geoname{Yangtze River}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "90",
day = "28",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030090",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/90",
abstract = "Coilia nasus is a typical anadromous migratory fish
found in the lower reaches of the Yangtze River. Every
year, C. nasus clusters offshore and swims upstream
along the Yangtze River into the tributaries and lakes
in the middle and lower reaches of the Yangtze River to
breed. In this study, female C. nasus were collected as
study subjects from the Chongming section of Shanghai,
the Taizhou section of Jiangsu, and the Anqing section
of Anhui. Their ovaries were used to examine tissue
sections and investigate gene expression, including the
follicle-stimulating hormone receptor (fshr), the
luteinizing hormone receptor (lhr), kisspeptin-1
(kiss1), and forkhead box l2 (foxl2), which are related
to reproductive development, while the serum levels of
estrogen (including estradiol, E2) and progestins
(including
17{\alpha},20{\beta}-dihydroxy-4-pregenen-3-one,
17{\alpha},20{\beta}-DHP) were also analyzed. Our
results showed that, first, the growth period of the
oocytes was small in stage II of ovarian development,
in which both E2 and 17{\alpha},20{\beta}-DHP levels
and gene expression were low. Then, in stage III, the
growth period of the oocytes became large, and the yolk
granules and oil droplets began to appear.
Simultaneously, E2 and the expression of kiss1 and
foxl2 were significantly elevated. Finally, stage IV
was the period of a large amount of accumulation of
nutrients in the oocytes, and 17{\alpha},20{\beta}-DHP
levels and the expression of fshr and lhr were
significantly elevated. These results enrich the
theoretical study of ovarian development in the natural
population of C. nasus, supplementing the biological
basis of C. nasus reproduction and scientifically
supporting the study of C. nasus population ecology and
resource conservation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhao:2024:MMG,
author = "Yun Zhao and Junming Zhou",
title = "Molecular Mechanisms of Growth Differences in
\bioname{Gymnocypris przewalskii} and
\bioname{Gymnocypris eckloni} through a Comparative
Transcriptome Perspective",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "89",
day = "28",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030089",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/89",
abstract = "Genetic composition plays a crucial role in the growth
rate of species, and transcriptomics provides a potent
tool for studying genetic aspects of growth. We
explored the growth rates and transcriptomes of the
Cyprinids G. przewalskii (GP) and G. eckloni (GE). A
total of 500 individuals of G. przewalskii and G.
eckloni, matched in size, were separately cultured for
9 months in six cement tanks (each group with three
replicates). Growth indices were measured, revealing
that the growth rate of GE was greater than that of GP
(p {$<$} 0.05), while there was no significant
difference in survival rates (p {$>$} 0.05).
Simultaneously, we conducted RNA-Seq on the muscles of
both GP and GE. The results indicated a significant
difference of gene expression between GP and GE,
identifying 5574 differentially expressed genes (DEGs).
Quantitative real-time reverse
transcription--polymerase chain reaction of 10 DEGs
demonstrated consistency in expression profiles with
the results from the RNA-Seq analysis. The DEGs were
significantly enriched in glycolysis/gluconeogenesis
(ko00010), arachidonic acid formation (ko00061),
arginine biosynthesis (ko00220), and the MAPK
(ko04013), PI3K-Akt (ko04151), mTOR (ko04150), and
TGF-{\beta} (ko04350) signal pathways, as revealed by
Gene Ontology (GO) enrichment and the Kyoto
Encyclopedia of Genes and Genomes (KEGG) pathway
analysis. This study also identified some
growth-related DEGs, such as IGF2, Noggin, Decorin and
others. Notably, the low expression of IGF2 may be a
factor contributing to the slower growth of GP than
GE.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wei:2024:EFT,
author = "Yuchong Wei and Aixing Ma and Ya Deng and Minxiong Cao
and Ying Hu and Zhaoyi Cheng and Lijun Zhu",
title = "Effects of Flow Turbulence on the Entire Development
Process of Drifting Fish Eggs",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "88",
day = "27",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030088",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/88",
abstract = "Excessive flow turbulence poses a threat to the
development of drifting fish eggs, leading to mortality
or developmental malformations and ultimately depleting
early fish resources. Currently, there is a scarcity of
quantitative studies investigating the effects of flow
turbulence on the entire process of drifting fish egg
development, from fertilized egg division to hatching.
In this paper, the effects of different flow turbulence
conditions (FTCs), including turbulent kinetic energy
and shear stress, and action times on different stages
of fish egg development were quantitatively explored
using a transverse-oscillating-grids turbulence tank.
Empirical formulas were established to predict the
proportion of normal fish egg development under
different FTCs within a selected range. The research
findings provide a quantitative basis for protecting
early fish resources, mitigating the biological
invasion of specific fish, constructing fish-breeding
facilities, and ensuring safe transfer and
transportation.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Laskar:2024:ITC,
author = "Boni Amin Laskar and Dhriti Banerjee and Sangdeok
Chung and Hyun-Woo Kim and Ah Ran Kim and Shantanu
Kundu",
title = "Integrative Taxonomy Clarifies the Historical Flaws in
the Systematics and Distributions of Two Osteobrama
Fishes ({Cypriniformes: Cyprinidae}) in
\geoname{India}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "87",
day = "27",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030087",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/87",
abstract = "The taxonomy and geographical distributions of
Osteobrama species have historically posed challenges
to ichthyologists, leading to uncertainties regarding
their native ranges. While traditional taxonomy has
proven valuable in classification, the utility of an
integrated approach is restricted for this particular
group due to limitations in combining information from
biogeography, morphology, and genetic data. This study
addresses the taxonomic puzzle arising from the recent
identification of Osteobrama tikarpadaensis in the
Mahanadi and Godavari Rivers, casting doubt on the
actual distribution and systematics of both O.
tikarpadaensis and Osteobrama vigorsii. The research
reveals distinctions among specimens resembling O.
vigorsii from the Krishna and Godavari riverine
systems. Notably, specimens identified as O. vigorsii
from the Indian Museum exhibit two pairs of barbels,
while those from the Godavari River in this study are
identified as O. tikarpadaensis. Inter-species genetic
divergence and maximum likelihood phylogeny provide
clear delineation between O. vigorsii and O.
tikarpadaensis. The study suggests that O. vigorsii may
be limited to the Krishna River system in southern
India, while O. tikarpadaensis could potentially extend
from the Mahanadi River in central India to the
Godavari River in southern India. Proposed revision to
morphological features for both species, accompanied by
revised taxonomic keys, aim to facilitate accurate
differentiation among Osteobrama congeners. The data
generated by this research provide a resource for
future systematic investigations into cyprinids in
India and surrounding regions. Further, the genetic
diversity information obtained from various riverine
systems for Osteobrama species will be instrumental in
guiding aquaculture practices and formulating effective
conservation action plans.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2024:AFS,
author = "Jingjing Wu and Jinghua Fu and Dingkang Zhou and
Jiasen Huang and Minjun Xu",
title = "Assessment of Fish Species in \geoname{Wanlv Lake},
the Largest Drinking Water Source in \geoname{South
China}, by Environmental {DNA} Metabarcoding
Technology",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "86",
day = "24",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030086",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/86",
abstract = "As the largest lake in South China, Wanlv Lake is also
an important drinking water source for Guangdong and
Hong Kong and is responsible for water supplying of
more than 40 million people in the Guangdong-Hong
Kong-Macao Greater Bay Area. The study of fish
diversity in Wanlv Lake can help us to effectively
understand the changes and states of its aquatic
ecosystem and provide scientific basis for natural
ecological protection, biodiversity, sustainable use
and scientific management of fishery resources.
However, research on the lake's fish is scarce. The
high throughput environmental DNA metabarcoding
technology (eDNA technology) of the mitochondrial 12S
rRNA gene sequence was herein used to conduct a
preliminary exploration of Wanlv Lake fish diversity. A
total of 10 sampling sites were set up to monitor fish
diversity and analyze the composition and richness of
the species. The results revealed a total of 83 genera
in 42 families and 17 orders of fish in the lake, with
the highest proportion found in Cypriniformes (38
species), accounting for 45.24\% of the total. Five
alien species and eight rare and endangered species
were also detected. The proportion of invasive species
was 5.95\%, and the combined proportion of rare and
endangered species was 9.52\%. The results indicated
that Wanlv Lake serves as an important drinking water
source, and overall, the condition of fish is
satisfactory. However, proactive measures should be
implemented to control the overpopulation of invasive
species, as their unchecked proliferation may lead to a
decline in species abundance, particularly among
endangered species. This was the first overall
assessment report on fish of the Wanlv Lake via 12S
rRNA; the results herein lay a foundation for water
quality assessment of Wanlv Lake as a drinking water
source.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2024:AOA,
author = "Junxiang Chen and Shilong He and Zelong Zhang and
Jiajun Li and Xiuxia Zhang and Juntao Li and Jiarui Xu
and Peihua Zheng and Jianan Xian and Yaopeng Lu",
title = "Application of Organic Acid Salts as Feed Additives in
Some Aquatic Organisms: Potassium Diformate",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "85",
day = "24",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030085",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/85",
abstract = "Antibiotics are the primary measures employed in the
prevention and treatment of diseases in aquaculture.
However, the frequent utilization of antibiotics can
significantly impact the growth and reproduction of
aquatic organisms, resulting in water pollution. The
European Union (EU) has prohibited antibiotic additives
in animal feed. Potassium diformate (KDF) represents
the first non-antibiotic feed additive approved by the
EU as a viable alternative to antibiotics. Its
application in animal nutrition has been validated,
demonstrating beneficial health effects. This article
reviews the physicochemical properties, biological
functions, synthesis conditions, and applications of
KDF in aquaculture and looks toward to its future
potential. It also provides insights into improving the
utilization of KDF in aquaculture practices.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2024:DMR,
author = "Feipeng Li and Siyu Xie and Mingzhu Wang and Ling Chen
and Haixiang Yu",
title = "Distribution and Management of Residual Antibiotics in
the \bioname{Litopenaeus vannamei} Shrimp Farming
Environment: Recommendations for Effective Control",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "84",
day = "23",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030084",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/84",
abstract = "This study specifically focused on Litopenaeus
vannamei and examined the distribution of residual
antibiotics in various components of shrimp ponds
throughout an aquaculture cycle. The findings revealed
that aquaculture feed served as the primary source of
antibiotics, continuously introducing them into the
ponds throughout the entire production cycle. A
multimedia distribution model for antibiotics in the
ponds was established based on the principle of mass
balance. The distribution characteristics of six
antibiotics with higher levels in the feed, namely,
sulfamethoxazole (SMX), norfloxacin (NOF), levofloxacin
(LEOF), tetracycline (TC), oxytetracycline (OTC), and
chlortetracycline (CTC), were investigated in the pond
water, sediment, and shrimp. At the end of the
cultivation period, the total antibiotic residues
accounted for 65~80\% in various media, with the
sediment containing 50~60\% of the distribution
proportion (p {$<$} 0.01), which was identified as the
primary reservoir for most antibiotics, with LEOF and
NOF accounting for the highest proportions (45.78\% and
50.29\%, respectively). Based on the model's findings
and the allowable daily dosage of antibiotics,
recommendations were made for the effective control of
antibiotic residues in shrimp farming management. To
address the significant net loss of sulfonamides (SAs)
and tetracyclines (TCs) in aquaculture production, it
is crucial to carefully regulate their dosages and
administration methods. Implementing eco-friendly
additives and regularly cleaning surface sediments can
aid in reducing antibiotic residue levels in various
environmental media, thereby mitigating the
environmental impact on aquaculture production
activities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xuan:2024:DCD,
author = "Wendan Xuan and Hongliang Zhang and Haobo Zhang and
Tian Wu and Yongdong Zhou and Wenbin Zhu",
title = "Distribution Characteristics and Driving Factors of
\bioname{Collichthys lucidus} Species in Offshore
Waters of \geoname{Zhejiang Province, China}",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "83",
day = "23",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030083",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/83",
abstract = "Collichthys lucidus is a small fish found in offshore
waters that is economically important for China. It is
imperative to understand its distribution
characteristics and driving factors. Based on survey
data of trawl fishery resources offshore of Zhejiang
province, China, in spring (April) and autumn
(November) from 2018 to 2022, the spatial and temporal
distributions of C. lucidus in this area were analyzed.
The random forest (RF) model was used to determine the
important marine factors affecting the distribution of
C. lucidus. The relationship between the distributions
of the important variables was analyzed. The results
showed that C. lucidus was mainly distributed in
coastal waters. The tail density of the species
exhibited obvious seasonal variation and was
significantly greater in autumn than in spring. The
most important factor affecting the distribution of
this species in spring and autumn was water depth. The
bottom temperature, bottom salinity and dissolved
oxygen concentration were also important influencing
factors. The importance of these factors differed among
the different seasons, while the chlorophyll a
concentration and pH had no significant effect on the
species distribution. This study revealed the
distribution pattern of C. lucidus in offshore waters
of Zhejiang Province and the influence of important
marine factors on its distribution. This study can
enrich the survey data on C. lucidus and provide basic
data for its scientific management and protection.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vetorelli:2024:IAR,
author = "Michelle Pinheiro Vetorelli and Laurindo Andr{\'e}
Rodrigues and Janaina Mitsue Kimpara and Wagner C.
Valenti",
title = "Intensification of \geoname{Amazon River} Prawn
Hatchery",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "82",
day = "22",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030082",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/82",
abstract = "The effects of the intensification on the performance
of the Amazon River prawn hatchery carried out in a
simple recirculation system were investigated. Newly
hatched larvae were stocked in 120 L tanks at 80, 100,
120 and 140 larvae L {$-$1} in a closed recirculating
system. The experiment used a randomized block design
with five replicates. An exponential equation was
adjusted to express the relationship between the
stocking density and productivity (postlarvae L
{$-$1}). The development, larval quality, survival and
postlarval (PL) dry weight did not significantly differ
among the treatments (p {$>$} 0.05). When 80 larvae
were stocked, the productivity (54 {\pm} 11 PL L
{$-$1}) was lower than those at higher densities (p
{$<$} 0.05). Stocking 120 and 140 larvae L {$-$1}
resulted in higher productivities (75 {\pm} 18 and 80
{\pm} 17 PL L {$-$1}, respectively) with a lower use of
Artemia nauplii to produce each postlarvae (~1200
Artemia nauplii PL {$-$1}). The maximum mean M.
amazonicum postlarval production estimated by the
exponential model was 93 PL L {$-$1}. This means that
despite the increase in stocking density, productivity
tends to stabilize. The results showed that M.
amazonicum tolerates high intensification in
recirculating hatchery systems based on a crushed shell
bed biofilter, and the intensification optimizes
Artemia use.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2024:PFG,
author = "Tianjiao Zhang and Jia Xin and Wei Yu and Hongchun
Yuan and Liming Song and Zhuo Yang",
title = "Predicting the Fishery Ground of Jumbo Flying Squid
(\bioname{Dosidicus gigas}) off \geoname{Peru} by
Extracting Features of the Ocean Environment",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "81",
day = "21",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030081",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/81",
abstract = "We introduce a novel method that combines satellite
data, advanced clustering techniques, machine learning
feature extraction, and statistical models to enhance
fishery forecasting accuracy. Focusing on jumbo flying
squid in the southeast Pacific Ocean near Peru, we
utilize MODIS-Aqua and MODIS-Terra satellite data on
sea surface temperature (SST) to construct a deep
convolutional embedded clustering (DCEC) model and
extract the monthly SST features (FM) based on an
optimized number of clusters determined by the
Davies--Bouldi index (DBI). We use the extracted FM to
construct a series of Generalized Additive Models (GAM)
to forecast the catch per unit effort (CPUE) of jumbo
flying squid within a spatial resolution of {0.5$^\circ
$} $ \times $ {0.5$^\circ $}. Our results demonstrate
the following findings: (1) The SST feature clusters
obtained through the DCEC model could capture the SST
monthly variations; (2) The GAM models with FM
outperform the models with the traditional monthly
average SST in terms of predictive accuracy; (3) Using
both FM and average SST together can further improve
model performance. This study demonstrates the
effectiveness of the DCEC combined with DBI in
extracting marine environmental features and highlights
the ocean environment feature extraction method to
enhance the precision and reliability of fishery
forecasting models.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Guo:2024:EAS,
author = "Kun Guo and Mumin Shi and Xiaoli Huang and Liang Luo
and Shihui Wang and Rui Zhang and Wei Xu and Guoliang
Ruan and Zhigang Zhao",
title = "The Effect of Artificial Substrate and Carbon Source
Addition on Bacterial Diversity and Community
Composition in Water in a Pond Polyculture System",
journal = j-FISHES,
volume = "9",
number = "3",
pages = "80",
day = "20",
month = feb,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9030080",
ISSN = "2410-3888",
bibdate = "Sat Apr 6 15:27:36 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/3/80",
abstract = "The use of artificial substrates and biofloc
technology can favor fish culture and improve water
quality. The aim of this study was to evaluate whether
artificial substrates and carbon source additions
modify the microbial activity of water bodies. The
diversity and structure of microflora in the water
after adding artificial substrates and carbon sources
to the ponds were analyzed using high-throughput
sequencing based on the V3-V4 region of 16S rRNA genes.
The results showed that there was no difference in the
richness and diversity of intestinal microflora between
the control and experimental groups. Principal
coordinate analysis (PCoA) and nonmetric
multidimensional scaling (NMDS) showed that artificial
substrate and carbon source addition changed the
structure of the microflora. The results of a linear
discriminant analysis (LDA) effect size (LefSe)
indicated 11 biomarkers in the EG\SGMLentity{717}st
group. Spearman correlation heatmap analysis showed
that environmental factors affected the bacterial
communities, and the results of a redundancy analysis
indicated that chemical oxygen demand was a critical
factor in controlling the bacterial communities in the
water. These results provide an understanding of the
effect of artificial substrate and carbon source
addition on bacterial diversity and community
composition in water.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Nekrasova:2024:API,
author = "Oksana Nekrasova and Mihails Pupins and Volodymyr
Tytar and Leonid Fedorenko and Oleksandr Potrokhov and
Arturs {\v{S}}kute and Andris {\v{C}}eir{\=a}ns and
Kathrin Theissinger and Jean-Yves Georges",
title = "Assessing Prospects of Integrating \geoname{Asian}
Carp Polyculture in \geoname{Europe}: a Nature-Based
Solution under Climate Change?",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "148",
day = "22",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040148",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/148",
abstract = "Aquaculture's role in addressing food security has
grown, with a spotlight on Asian carp species.
Polyculture, i.e. cultivating multiple fish species in
a single system, is being increasingly adopted for its
resource efficiency and economic benefits. This
practice for Asian fish for food and ornamental
purposes is gaining traction in Europe despite their
invasive potential. Rising temperatures due to climate
change offer an opportunity for thermophilic Asian
carps (Hypophthalmichthys molitrix and Aristichthys
nobilis). Using GIS modeling (Maxent), we identified
the possible settlement of Asian carp in Northern
Europe amidst climate change. We analyzed carp global
distribution centers, assessed the potential carp
spread in Europe, and evaluated their potential
suitability for polyculture systems. By 2050, H.
molitrix may extend its range to 58--{62$^\circ $} N
latitude, with a potential 1.7-fold habitat increase,
while A. nobilis, which are more heat-tolerant, may
move north to 52--{58$^\circ $} N latitude, with a
1.3-fold potential increase. Despite the slight
ecological differences in their native habitats, niche
modeling indicates that these carp can occupy similar
niches in Europe (proven statistically). The
eventuality of using Asian species for polyculture in
Europe presents both opportunities and challenges in
the face of a changing climate as long as invasion
risks are prevented. Envisaging such polyculture, yet
very carefully for the protection of ecosystems, can
help food security.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ahn:2024:EDC,
author = "Hyojin Ahn and Michael J. Miller",
title = "Environmental {DNA} Characterization of the Fish
Species Composition in the \geoname{Mukawa River} and
Adjacent Habitats",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "147",
day = "21",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040147",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/147",
abstract = "The diverse freshwater fish fauna of the Japanese
archipelago is distributed among four main island
landmasses, which include Hokkaido in the north, with
many diadromous species. One relatively well-preserved
river drainage along the southern coast of Central
Hokkaido is the Mukawa River. Fish fauna surveys in the
Mukawa River were mostly in downstream areas and the
fish diversity is not well-documented among the upper,
lower river, and coastal environments. Fish communities
in the river, estuary, and sea were sampled using eDNA
analysis to evaluate upstream and downstream species
detections, and tidal and spatial detection variation
near the river mouth. The number of species was higher
at the river mouth and nearshore sites compared to the
river and offshore. Fish detections reflected life
history categories (freshwater resident, diadromous,
brackish, or marine) and the environments. Similarity
analysis showed that fish species compositions were
divided into (1) upstream and midstream, (2) downstream
and river mouth, (3) adjacent shore, and (4) offshore.
Salmonid, cyprinid, loach, and sculpin species were
detected in the river, compared to a mixture of species
downstream and along the coast. This rapid assessment
type study demonstrated that eDNA survey methodology
would be effective for multiple river comparative
surveys, seasonality studies, or evaluating possible
effects of cross-river weirs or dams.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Samarajeewa:2024:SPU,
author = "Upali Samarajeewa",
title = "Safety, Processing, and Utilization of Fishery
Products",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "146",
day = "21",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040146",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/146",
abstract = "Global marine fish harvests have reached a plateau
over the last decade, and efforts to increase
aquaculture tend to face limitations in terms of water
resources and contamination problems. Of the current
fish harvest, at least 50\% is discarded as waste. The
current situation requires efforts to process,
preserve, and utilize the fish capture to minimize
waste. Chemical and microbiological contamination limit
the utilization of harvested fish. There is a need to
improve fish preservation to minimize spoilage and to
process them into more appealing products. Instead of
resorting to individual food-processing methods, the
efficiency of processing could best be increased by a
combination of conventional and modern processing
methods or by combinations of modern processing
methods. Fish waste is a rich source of oils containing
essential fatty acids, polypeptides, and amino
polysaccharides that could be utilized through the
upscaling of current scientifically proven methods to
new processing technologies. The separation of
collagens, gelatins, bioactive peptides, edible fish
oils, and chitosan form the primary stages in the
utilization of fish waste. The products need
purification to meet food quality and safety standards
and to have desirable industrial characteristics. The
diversity of information and products generated through
new methods require advanced data handling and
prediction systems, such as artificial intelligence, to
address food safety and to derive the best out of fish
processing and utilization.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pena-Almaraz:2024:BCE,
author = "Omar Alejandro Pe{\~n}a-Almaraz and Manuel Alejandro
Vargas-Ceballos and Edilmar Cort{\'e}s-Jacinto and
Fernando Vega-Villasante",
title = "Biochemical Composition of Eggs, Larvae and Tissues of
\bioname{Macrobrachium tenellum} Females Fed Diets with
Different Lipid and Protein Levels",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "145",
day = "21",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040145",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/145",
abstract = "One way to approach the nutritional requirements of
native shrimp, necessary to consolidate their culture,
is to know their biochemical composition. The effect of
feeding two levels of lipids (4 and 12\% L) and four
levels of proteins (30, 35, 40 and 45\% P) in M.
tenellum females was evaluated with respect to the
biochemical composition of their eggs (EG), larvae
(LR), gonad (GO) and hepatopancreas (HP). Total protein
(TP), total carbohydrate (TC) and total lipid (TL) were
estimated. In EG, L and P levels influence TP and TL;
TP increases in diets higher than P35. In LR, there are
no differences (p {$>$} 0.05) in TP and in TL, only
diets L4P40 and L12P30 were different (p {$<$} 0.05).
In GO, there is no trend in TP differences; in TC there
was variation in the range of the data and TL was
higher in L4P30 and L4P35. In HP, the diets with L4
obtained the highest TP values (p {$<$} 0.05); the L12
diets were higher in TL (p {$<$} 0.05). In general,
diets with an inclusion of L12 showed the highest TP,
TC and TL means, within this lipid level the P30 diet
stood out; therefore, it is recommended to use a diet
with L12P30 in the formulation of balanced feed for the
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{An:2024:CBC,
author = "Hyung-Eun An and Adeel Malik and Jeongho Lee and
Min-Ho Mun and Kang Hyun Lee and Hah Young Yoo and
Chang-Bae Kim",
title = "Comparison of Bacterial Community Composition in Gut
of \geoname{Chinese} Mitten Crabs from Three Distinct
Rivers in \geoname{Korea}",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "144",
day = "20",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040144",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/144",
abstract = "The Chinese mitten crab (CMC) also known as Eriocheir
sinensis has great significance in the aquaculture
industry. The bacterial communities inhabiting the
CMC's gut may differ depending on the host habitat and
can aid in their normal biological functioning. These
microbes are also known to have certain effects on
their flavor. In this study, we utilized MiSeq
high-throughput sequencing technology to explore the
diversity of bacterial communities in the gut of CMCs
from three different geographical locations in Korea:
the Geum (GD), Han (HD), and Tamjin (TD) rivers.
Although most of the environmental parameters were
similar at the three sites, significant differences in
conductivity (CDS), dissolved oxygen (DO), and salinity
were observed. The results show that CMCs sampled from
these locations exhibited distinct microbial
composition and abundance. For example, the genus
Candidatus Hepatoplasma displayed significantly higher
abundance in CMCs from HD than those in the other
locations, suggesting nutritional stress. Similarly,
the crabs collected from TD showed a higher abundance
of pathogenic Helicobacter than those from HD and GD
sites. We also observed differences in the amino acid,
nucleotide, and lactic acid concentrations between
different tissues such as the muscle, hepatopancreas,
and testis of CMCs. However, only small differences
were observed when these characteristics were compared
in CMCs from different locations. Our results offer
important insights into the intestinal bacterial
composition in CMCs which in turn may help in designing
better culturing strategies for these important species
of crabs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ghahvei:2024:HIN,
author = "Younes Ghahvei and Mohammad Mirzaei and Shahrzad Azizi
and Shadi Hashemnia and Shokoofeh Shamsi",
title = "Health Insights from Nematode Larval Characterization
in Greater Lizardfish, \bioname{Saurida tumbil}
({Bloch}, 1795) ({Teleostei, Synodontidae})",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "143",
day = "20",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040143",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/143",
abstract = "Fish health is of significant ecological and economic
importance. In response to public observations of
parasite-like structures in a popular edible fish, this
study aimed to characterize nematode larvae commonly
found in the muscle and body cavity of Saurida tumbil
(Bloch, 1795) , a commercially important fish species
inhabiting the Persian Gulf and Oman Sea. This fish,
locally known as Hasoom, holds substantial culinary
importance, being a staple in the diets of millions
residing in countries around the Persian Gulf. A total
of 458 Saurida tumbil specimens were obtained from fish
markets between June 2022 and May 2023. Subsequent
examination revealed the presence of a total of 6132
nematode larvae. Nematodes found in the body cavity
were identified as belonging to the genus
Hysterothylacium sp., family Raphidascarididae, while
those in the muscle were identified as Anisakis sp.
larval type, family Anisakidae. Histopathology results
suggested that these parasites may have adverse health
impacts on their fish host. Notably, both nematode
genera were found in the third larval stage, which is
known to be the infective stage for anisakidosis. Given
the reported cases of anisakidosis among people living
in the study region, it is strongly recommended that
fish be properly cooked before consumption to mitigate
health risks.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Erlandsson:2024:UQI,
author = "Ann Erlandsson and Giedr{\.e} A{\v{s}}monait{\.e} and
Bror Jonsson and Larry Greenberg",
title = "Using {qPCR} to Identify Potential Effects of Thermal
Conditions during Embryogenesis on Mitochondrial {DNA}
Copy Number in Juvenile Brown Trout \bioname{Salmo
trutta}",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "142",
day = "19",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040142",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/142",
abstract = "Changes in the number, structure, and function of
mitochondria during the early life stages of animals
can play an important role for an organism's metabolic
rate, growth, and health. Previous studies have shown
that juvenile brown trout (Salmo trutta) subjected to
elevated temperatures during the embryonic stage
respond phenotypically with a reduced metabolic rate.
The aim of this study was to explore if embryonic
temperature affects the mitochondria content of young
brown trout and as such explains the previously found
differences in metabolic rates. Here, we optimize a
quantitative PCR (qPCR) method for the mitochondria
cytochrome c oxidase subunit I gene, and then use the
method as a proxy for mitochondrial DNA content. We
hypothesize that young trout subjected to elevated
temperatures during the embryonic stage respond
phenotypically with a reduced mitochondrial DNA
content. To test this hypothesis, we subjected brown
trout to either control ambient (4.4 {\pm} 1.5 {$^\circ
$}C) or elevated temperatures (7.1 {\pm} 0.6 {$^\circ
$}C) during embryogenesis. Subsequently, we extracted
DNA from liver and white muscle tissue of juvenile
brown trout from the two different incubation
temperature treatments and successively optimized qPCR
for mitochondrial DNA. We found that the amount of
mitochondria DNA in liver tissue was 18 times higher
than in white muscle tissue, but there was no
significant difference in mitochondria content in liver
or muscle tissue between brown trout exposed to
elevated and ambient control temperatures during
embryogenesis. We conclude that reduced metabolic rate
is not likely associated with mitochondria DNA content.
We also suggest that qPCR is a simple and
cost-effective method to quantify mitochondria DNA in
frozen and partly degraded tissue from different
treatment groups and a useful proxy for identification
of differences in mitochondria number.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yang:2024:CMP,
author = "Zhigang Yang and Qingqing Jiang and Wuxiao Zhang and
Silei Xia and Hongyan Tian and Fei Liu and Wenping Yang
and Yebing Yu and Yanmin Wu and Yongzhi Zhu and
Zhiqiang Xu and Zemao Gu and Aimin Wang and Aqin Chen",
title = "Comparison of Morphometric Parameters, Nutritional
Composition, and Textural Properties of Seven
Crustaceans Species",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "141",
day = "17",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040141",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/141",
abstract = "In order to study the muscle quality of different
crustaceans, we aim to provide a comprehensive
assessment of their muscle quality characteristics as a
theoretical reference. In this work, seven major
species of farmed and consumer crustaceans were
selected, including crayfish (Procambarus clarkii),
freshwater prawns (Macrobrachium rosenbergii), pacific
white shrimp (Litopenaeus vannamei), black tiger shrimp
(Penaeus monodon), kuruma prawns ( Penaeus japonicus),
river prawns (Macrobranchium nipponense), and Chinese
shrimp (Penaeus chinensis). Their morphometric
parameters, nutritional composition, textural
properties, and physical and chemical indexes were
comparatively analyzed. The results showed that the
meat content (MC, about 14.78\%) of crayfish was
significantly lower than that of the other six species.
By contrast, pacific white shrimp had the highest MC,
although the MCs of black tiger shrimp, kuruma prawns,
and Chinese shrimp are greater than 40\%. All seven
crustacean species were high in protein and low in fat,
while pacific white shrimp had the highest crude
protein, crude lipid, and crude ash content compared to
the other crustaceans. The content of threonine (Thr)
was the highest in crayfish. The content of methionine
(Met) and lysine (Lys) was the highest in freshwater
prawns. The content of isoleucine (Ile), leucine (Leu),
and non--essential amino acid (NEAA) was the highest in
pacific white shrimp. C18:2n-6 (linoleic acid, LA) was
the highest in freshwater prawns and pacific white
shrimp; C18:3n-3 (linolenic acid, LNA), C20:4n-6
(arachidonic acid, AA), unsaturated fatty acids (UFA),
and monounsaturated fatty acids (MUFA) were all the
highest in crayfish; and polyunsaturated fatty acids
(PUFA) was the highest in freshwater prawns, but the
content was not significantly different from crayfish,
pacific white shrimp, black tiger shrimp, and Chinese
shrimp. Pacific white shrimp had the highest values for
hardness, cohesiveness, gumminess, and chewiness. The
redness values of crayfish, black tiger shrimp, and
Chinese shrimp were significantly higher than those of
the other three species except kuruma prawns. Compared
to other crustaceans, river prawns had the highest drip
loss and cooking loss. Black tiger shrimp and Chinese
shrimp had the lowest cooking loss rates. The research
shows that the tail muscle of the seven species of
crustaceans is rich in protein, essential amino acids,
unsaturated fatty acids and low in fat, representing a
high-quality protein. Among these crustaceans, the main
essential amino acids and essential fatty acids in the
tail muscle of pacific white shrimp, freshwater prawns,
and crayfish are higher in content and better in
nutritional value.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luo:2024:TGS,
author = "Zhang Luo and Xiaohui Bai and Shuang Hao and Mengyu
Wang and Yongjiang Wu and Hanchang Sun",
title = "Two Genotypes of \bioname{Streptococcus iniae} Are the
Causative Agents of Diseased Ornamental Fish, Green
Terror Cichlid (\bioname{Aequidens rivulatus})",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "140",
day = "17",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040140",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/140",
abstract = "Green terror cichlid (Aequidens rivulatus) is a
popular tropical freshwater ornamental fish. In 2021,
an unknown disease was observed in cultured A.
rivulatus in Tianjin, China, with a cumulative
mortality rate of 25\% within 7 days of onset. The main
clinical signs were scale loss, skin ulceration, and
slight bleeding. Histopathological observation revealed
obvious damage to the liver, spleen, and kidney of
diseased fish. In addition, abundant granulomas were
observed in the spleen and head kidney of the diseased
fish. To define the potential pathogens from A.
rivulatus, bacteria were isolated from the visceral
tissue of diseased fish with conventional methods. An
artificial infection experiment was carried out to
prove the pathogenicity of the isolated bacteria. The
strains HG-2021-1 and HG-2021-3 were isolated from
diseased fish and identified as being responsible for
the disease. They were identified as Streptococcus
iniae based on physiological and biochemical tests,
lctO gene detection, and 16S rRNA gene sequence
analysis. According to the result of multilocus
sequence typing (MLST), HG-2021-1 and HG-2021-3 belong
to different genotypes of S. iniae. Furthermore, they
were found to contain the virulence genes pgmA, scpI,
cpsD, and pdi, and the median lethal dose (LD 50) for
A. rivulatus was 1.8 $ \times $ 10 6 Colony-Forming
Units (CFU)/mL and 6.6 $ \times $ 10 6 CFU/mL,
respectively. To our knowledge, this is the first
report of fish coinfected by two genotypes of S.
iniae.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lima:2024:NFI,
author = "Adriana Ferreira Lima and Anderson Guilherme Pereira
dos Reis and Vladimir Eliodoro Costa and Wagner Cotroni
Valenti",
title = "Natural Food Intake and Its Contribution to Tambaqui
Growth in Fertilized and Unfertilized Ponds",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "139",
day = "16",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040139",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/139",
abstract = "Natural food available in ponds can complement
formulated feed in fed aquaculture. This study
elucidated the natural food intake and its contribution
to tambaqui (Colossoma macropomum) growth in fertilized
and unfertilized ponds, using stable isotope and
stomach contents analyses. Additionally, it described
the impact of fertilization management on natural food
availability, fish performance, and production costs.
Tambaqui juveniles (93.8 {\pm} 15.0 g) were stocked
(0.55 fish/m 2) in fertilized (Fert) and unfertilized
(NoFert) ponds (600 m 2) for a culture period of 10
months in quadruplicate. A lower food conversion ratio
was observed in Fert ponds. The main natural food items
ingested by tambaqui were insects, vegetables, and
cladocerans. Plankton contributed 39.4\% and 10.7\% of
muscle formation in Fert and NoFert ponds,
respectively. Pond fertilization (2.45 g of nitrogen
and 0.80 g of phosphorus per square meter every two
weeks) did not significantly affect fish growth,
survival, or productivity but had a slightly influence
on water quality parameters. However, fertilization
increased the zooplankton density (through
phytoplankton) in the water, thereby increasing
autochthonous food availability for tambaqui
consumption. This species demonstrates the ability to
alternate between natural food sources and commercial
feed without compromising its development.
Consequently, tambaqui exhibits suitability for farming
within restorative and integrated aquaculture systems
as well as intensive systems reliant on commercial
feed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2024:EDF,
author = "Hongquan Wang and Zheming Zhang and Feilong Li and
Liang Hu and Tiaoyi Xiao and Yurong Zhao and Mengxi
Yang",
title = "The Effects of Dietary Fermented Soybean Residue on
the Growth, Antioxidant Capacity, Digestive Enzyme
Activities, and Microbial Compositions of the Intestine
in \geoname{Furong} Crucian Carp (\geoname{Furong}
Carp[female-sign] $ \times $ Red Crucian Carp[male
sign])",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "138",
day = "16",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040138",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/138",
abstract = "An 8-week feeding experiment aimed to investigate the
effects of fermented soybean residue in diets on the
growth performance, serum antioxidant parameters,
intestinal digestive capacity, and microbial
composition of the hindgut of Furong crucian carp. The
feeds were formulated to contain 0\%, 6\%, 12\%, 18\%,
and 24\% fermented soybean residue (CON, FSR6, FSR12,
FSR18, and FSR24, respectively), respectively, to form
five diets (crude protein: 33\%; crude lipid: 5\%). The
results showed that incorporating 6\% fermented soybean
residue into the diet significantly increased the
weight gain rate (WGR) and specific growth rate (SGR)
and decreased the feed coefficient rate (p {$<$} 0.05).
Through regression analysis of WGR and SGR, the optimal
levels of the dietary fermented soybean residue of
Furong crucian carp were determined to be 6.78\% and
7.06\%. Incorporating fermented soybean residue into
the diet decreased the lipid content of the whole body
and the levels of glucose, total cholesterol, and
triglyceride in the serum of Furong crucian carp. The
inclusion of 6\% and 12\% fermented soybean residue in
the diet markedly increased the antioxidant capacity,
intestinal amylase activity, and intestinal villous
height of Furong crucian carp (p {$<$} 0.05). At the
6\% level, fermented soybean residue significantly
increased the abundance of Romboutsia and
Clostridium_sensu_stricto_1 while significantly
decreasing the abundance of Vibrio (p {$<$} 0.05),
indicating that a 6\% level of fermented soybean
residue was beneficial for intestinal health. In
conclusion, incorporating 6--7\% fermented soybean
residue into the diet of Furong crucian carp was
recommended.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sievers:2024:MSC,
author = "Katie T. Sievers and Eva C. McClure and Rene A.
Abesamis and Garry R. Russ",
title = "Multi-Scale Coral Reef and Seascape Habitat Variables
Combine to Influence Reef Fish Assemblages",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "137",
day = "15",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040137",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/137",
abstract = "While benthic characteristics of coral reef habitats
are a major driver of the structure of coral reef fish
assemblages, non-reef habitats adjacent to coral reefs
(e.g., mangroves, seagrass beds, and macroalgal beds)
can affect reef fish assemblages. Here, we investigate
how reef fish assemblages respond to local-scale
benthic habitats within a coral reef and larger-scale
adjacent seascape features (habitats within 500 m of
coral reefs) on Siquijor Island in the Philippines. We
examined an abundance of species for the entire reef
fish assemblage and within the assemblages of
parrotfishes (subfamily Scarinae) and wrasses (family
Labridae). Five distinct habitat types were identified
in a cluster analysis, which incorporated benthic
characteristics within coral reefs and habitats
adjacent to coral reefs. We found that the diversity
and structure of coral reef fish assemblages were
affected by benthic characteristics within coral reefs
and also by benthic habitat types adjacent to coral
reefs. Individual species responses and juveniles of
certain species demonstrated uniquely high abundances
in habitat clusters characterized by the non-reef
habitats surrounding coral reefs. Considering coral
reef habitats and adjacent non-reef habitats as a
holistic, interconnected seascape will provide better
estimations of the drivers of the structures of coral
reef fish assemblages.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pinheiro:2024:RFS,
author = "Larissa da Costa Pinheiro and Beatrice Padovani
Ferreira and Paulo Em{\'\i}lio Santos and Ualerson
Peixoto and Bianca Bentes",
title = "Recent Findings on Spawning Patterns and
Recommendations for the Fishery Management of the
Southern Red Snapper --- \bioname{Lutjanus purpureus}
({Poey}, 1866) --- On the \geoname{Amazon Continental
Shelf of Brazil}",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "136",
day = "15",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040136",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/136",
abstract = "The southern red snapper, Lutjanus purpureus, is one
of the primary fishery resources of the Amazon
continental shelf. Due to the demand for specimens of
less than 900 g in weight, increased fishing effort
focusing on this size class has led to a drastic
decrease in catches. The present study was based on the
analysis of cellular structures in the female gonads
(formation of oocytes and other reproductive tissues)
to identify reproductive processes and evaluate the
effectiveness of current fishery management practices.
The presence of postovulatory follicles (POFs) peaked
in April, which was interpreted as a period of intense
spawning. In addition to POFs, hydrated oocytes peaked
in July, indicating either reproductive continuity or a
second peak, based on the presence of atretic oocytes
and atresia. Given that the local snapper fishery
preferentially targets very young individuals, an
extension of the closed season from March to July would
be recommendable to guarantee the reproductive
potential of the spawning stock.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tsotsios:2024:EFE,
author = "Dimitrios Tsotsios and Dimitrios K. Papadopoulos and
Maria V. Alvanou and Ioannis Georgoulis and Athanasios
Lattos and Basile Michaelidis and Konstantinos
Feidantsis and Ioannis A. Giantsis and John A.
Theodorou",
title = "Environmentally Friendly and Efficient Methods for
Mitigating the Density of Ascidian Fouling in
\geoname{Mediterranean} Mussel Farming",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "135",
day = "14",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040135",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/135",
abstract = "Ascidian biofouling generates significant challenges
to bivalve aquaculture. Their rapid spread across
almost every available surface leads to increased
maintenance costs and reduced yields in shellfish
farming. In addition, ascidians may introduce pathogens
or toxins, further compromising the health and
marketability of bivalve stocks and thereby
necessitating strict management strategies to manage
these impacts. The aim of this study was the evaluation
of different management practices for eliminating
ascidian fouling and the identification of the best
method for Mytilus galloprovincialis (Lamarck, 1819)
aquaculture farms. The effects of different
anti-fouling treatments as well as their interactive
outcomes were examined by conducting two experiments.
Various experimental procedures were applied, including
the temporally differential washing of mussels, air
exposure and immersion in 50 ppt and 70 ppt salinity
solutions, as well as the combination of these
applications. All treatments reduced the number of
ascidian colonies on mussel socks but at varying
proportions. Immersing mussel socks in a 70 ppt
salinity solution followed by air exposure for 1 day
was the most efficient method and led to a 93\%
eradication.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bogwald:2024:ECF,
author = "Isak B{\o}gwald and Simon Herrig and Alice Marie
Pedersen and Sileshi Gizachew Wubshet and Karl-Erik
Eilertsen",
title = "Effect of \bioname{Calanus finmarchicus} Hydrolysate
Inclusion on Diet Attractiveness for Whiteleg Shrimp
(\bioname{Litopenaeus vannamei})",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "134",
day = "13",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040134",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/134",
abstract = "Shrimp feed formulations have moved towards less fish
meal and more of the readily available and cheaper
plant proteins. To counteract the lower attractiveness
and palatability of plant proteins, feeds are
supplemented with ingredients known to have
chemoattractive properties that will increase feed
intake. This study investigated the putative
chemoattractive effect of Calanus finmarchicus
hydrolysate, when used as a dietary supplement in
shrimp feeds. C. finmarchicus is a zooplankton species
native to the northern Atlantic Ocean and is a novel
and sustainable raw material for shrimp feed products.
Diet attractiveness was evaluated in a 24-day feeding
trial with whiteleg shrimp (Litopenaeus vannamei) by
measuring the intake of 12 diets with various levels of
fish meal, calanus hydrolysate, and krill (Euphausia
superba) meal. Higher inclusion rates of both
ingredients resulted in increased feed intake, and
supplementing the high fish meal diet with calanus
hydrolysate gave a statistically significant higher
feed intake. Low molecular weight peptides,
chemoattractive amino acids, and the water-soluble
nature of the hydrolysate could explain the
chemoattractive properties observed in the study.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jareno:2024:EFA,
author = "Javier Jare{\~n}o and Guillermo
B{\'a}rcena-Gonz{\'a}lez and Jairo Castro-Guti{\'e}rrez
and Remedios Cabrera-Castro and Pedro L. Galindo",
title = "Enhancing Fish Auction with Deep Learning and Computer
Vision: Automated Caliber and Species Classification",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "133",
day = "13",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040133",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/133",
abstract = "The accurate labeling of species and size of specimens
plays a pivotal role in fish auctions conducted at
fishing ports. These labels, among other relevant
information, serve as determinants of the objectivity
of the auction preparation process, underscoring the
indispensable nature of a reliable labeling system.
Historically, this task has relied on manual processes,
rendering it vulnerable to subjective interpretations
by the involved personnel, therefore compromising the
value of the merchandise. Consequently, the
digitization and implementation of an automated
labeling system are proposed as a viable solution to
this ongoing challenge. This study presents an
automatic system for labeling species and size,
leveraging pre-trained convolutional neural networks.
Specifically, the performance of VGG16,
EfficientNetV2L, Xception, and ResNet152V2 networks is
thoroughly examined, incorporating data augmentation
techniques and fine-tuning strategies. The experimental
findings demonstrate that for species classification,
the EfficientNetV2L network excels as the most
proficient model, achieving an average F-Score of 0.932
in its automatic mode and an average F-Score of 0.976
in its semi-automatic mode. Concerning size
classification, a semi-automatic model is introduced,
where the Xception network emerges as the superior
model, achieving an average F-Score of 0.949.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2024:CGP,
author = "Ziyu Wang and Peiying Huang and Xiaoyong Li and
Jianmin Pei and Wenzhen Liu and Jiahao Hou and Linjie
Li and Hongxiang Fan and Liugen Zeng and Daxian Zhao",
title = "Comparison of Growth Performance, Nutritional
Composition, and Muscle Transcriptome between Two
Cultured Varieties of the \geoname{Chinese} Mitten Crab
(\bioname{Eriocheir sinensis})",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "132",
day = "9",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040132",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/132",
abstract = "This study evaluated the disparities in growth
performance and nutritional composition between two
common Chinese mitten crab varieties, ``Jianghai 21''
and ``Changjiang 2'', cultured in Jiangxi Province.
Over the breeding period, parameters such as body
weight, height, carapace length and width, and lengths
of the second and third legs exhibited increases.
Growth indices of ``Jianghai 21'' surpassed those of
``Changjiang 2'' during the intermediate and late
breeding phases. While ``Changjiang 2'' demonstrated
significantly higher crude lipid content than
``Jianghai 21'', the latter exhibited markedly higher
levels of total amino acids (TAAs), essential amino
acids (EAAs), and delectable amino acids. Although
overall nutrient composition did not significantly
differ, TAA and EAA contents in the hepatopancreas were
notably greater in ``Jianghai 21'' than in ``Changjiang
2''. Moreover, a total of 901 differentially expressed
genes (DEGs) were identified between the two varieties,
with 560 upregulated and 341 downregulated. These DEGs
were primarily associated with pathways related to
fatty acid degradation, metabolism, and growth
regulation. The observed variances in growth
performance and nutrient composition between ``Jianghai
21'' and ``Changjiang 2'' suggest potential regulation
by relevant genes. In summary, the results showed that
the growth performance of ``Jianghai 21'' is greater
than that of ``Changjiang 2'', offering valuable
insights for the selection of aquaculture varieties in
the region.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xiu:2024:EIP,
author = "Yunji Xiu and Jingyuan Yi and Ruixin Feng and Jiaxue
Song and Yunfei Pang and Peng Liu and Shun Zhou",
title = "Evaluation of Immune Protection of a Bivalent
Inactivated Vaccine against \bioname{Aeromonas
salmonicida} and \bioname{Vibrio vulnificus} in
Turbot",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "131",
day = "9",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040131",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/131",
abstract = "The Aeromonas salmonicida is responsible for causing
furunculosis in various fish species. Furunculosis is a
ubiquitous disease that affects the aquaculture
industry and causes the mass mortality of turbot.
Vibrio vulnificus is a pathogen that causes skin ulcers
and hemorrhagic septicemia in fish, resulting in
significant mortality in aquaculture. In this study, we
have established a bivalent inactivated vaccine against
A. salmonicida and V. vulnificus with
Montanide{\trademark} ISA 763 AVG as an adjuvant. This
bivalent inactivated vaccine was used to immunize
turbot by intraperitoneal injection, and the relevant
immune indexes were detected. The results demonstrate
that the bivalent inactivated vaccine exhibited a
relative percent survival (RPS) of 77\% following A.
salmonicida and V. vulnificus intraperitoneal
challenge. The vaccinated group exhibited higher levels
of acid phosphatase activity and lysozyme activity
compared to the control group. ELISA results showed a
significant increase in serum antibody levels in
immunized turbot, which was positively correlated with
immunity. In the kidney tissue, related immune genes (
TLR5, CD4, MHCI and MHCII) were up-regulated
significantly, showing that the vaccine can induce
cellular and humoral immune responses in turbot. In
conclusion, the bivalent inactivated vaccine against A.
salmonicida and V. vulnificus was immunogenic,
efficiently preventing turbot from infection, which has
the potential to be applied in aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chu:2024:RBM,
author = "Elaine Chu and Daniel Pauly",
title = "The Relationship between Mean Length at Maturity and
Maximum Length in Coral Reef Fish",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "130",
day = "9",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040130",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/130",
abstract = "This article proposes a mechanism that triggers first
maturation and spawning in coral reef (bony) fish,
which allows for predicting their length at first
maturity. Thus, mean lengths at first maturity (L m)
and the corresponding maximum lengths (L max) in 207
populations of 131 species of coral reef fish were
assembled and used to test the hypotheses that (a)
there is, in coral reef fish, a single value of a
size-related parameter acting as a trigger for their
maturation and eventual spawning, and (b) that this
single value is statistically the same as that
published previously for other bony fish. The results,
based on the assembled L m and L max data and on
estimates of the parameter D, which link the length of
fish with the relative surface of their gills, covered
44 families and L max values ranging from 1.8 to 181.6
cm and confirmed that the threshold in (a) exists.
Also, we assessed (in b) that this threshold value,
i.e., L max D /L m D = 1.35 ({\pm}0.02), is not
statistically different from similar estimates for
other groups of teleosts, notably semelparous
salmonids, cichlids, sturgeons and Chinese and Turkish
freshwater and marine fish. One implication is that
given ocean warming and deoxygenation, coral reef fish
will not only be smaller than they currently are, but
also mature and spawn at smaller sizes, and thus
produce fewer, smaller eggs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Muhammad:2024:VCA,
author = "Abdullateef Mukhtar Muhammad and Chang Yang and
Jingyuan Wang and Xianping Ge and Bo Liu and Linghong
Miao and Guodong Gao and Qunlan Zhou",
title = "Vitamin {C} Alleviates Intestinal Inflammation Caused
by \bioname{Aeromonas hydrophila} in Juvenile Blunt
Snout Bream (\geoname{Megalobrama amblycephala})",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "129",
day = "8",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040129",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/129",
abstract = "Vitamin C (VC) can be used to increase disease
resistance in practice in intensive aquaculture. But it
is still unconfirmed whether VC could alleviate
inflammation and what dosage is suitable. This study
investigated the effects of dietary VC on the immunity
and enteritis of juvenile blunt snout bream (
Megalobrama amblycephala) challenged with Aeromonas
hydrophila. The fish were fed with VC levels ranging
from 25.35 to 2231.98 mg/kg for 60 days. After that,
fish fed with 150.65 mg/kg, 573.79 mg/kg, and 2231.98
mg/kg VC were challenged with A. hydrophila orally to
simulate enteritis. The results showed that 285.39 and
573.79 mg/kg dietary VC significant improved growth
performance and feed utilization. Dietary VC (573.79
and 1133.79 mg/kg) significantly enhanced the serum
immune parameters, antioxidant enzymes' activities, and
relative gene expressions of ikb{\alpha} and cat in the
liver in the 60-day feeding trail. The survival rate
was significantly higher in 573.79 mg/kg dietary VC
group than the other two treatments. Cytokines were
activated after the bacteria challenge. Dietary 573.79
mg/kg VC caused significantly higher TNF-{\alpha} level
at 12 hpi and maintained a high level of IL-8 at 48 to
72 h compared to other treatments. In conclusion, more
than 500 mg/kg VC was needed to maintain the health of
blunt snout bream juveniles. Suitable VC would activate
cytokines to increase disease resistance along with
antioxidant enzymes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Stroe:2024:AGM,
author = "Desimira Maria Stroe and Mirela Cretu and Magdalena
Tenciu and Floricel Maricel Dima and Neculai Patriche
and George Tiganov and Lorena Dediu",
title = "Age, Growth, and Mortality of {Pontic} Shad,
\bioname{Alosa immaculata} {Bennett}, 1835, in the
\geoname{Danube River, Romania}",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "128",
day = "2",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040128",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/128",
abstract = "This study aimed to evaluate the growth, mortality
parameters, and exploitation rate of Pontic shad, Alosa
immaculata Benett, 1835, in the Danube River, Romania
(km 169--197). The sampling collection started with the
first signs of Pontic shad migration, on 4 March 2023,
and continued weekly until the beginning of June 2023,
when the last specimens were caught in the nets. The
estimation of the growth, mortality parameters, and
exploitation rate was done in FiSAT (FAO-ICLARM Stock
Assessment Tools). The von Bertalanffy growth equation
was estimated at L {\infty} = 36.75 cm, the growth
coefficient was k = 0.68 year {$-$1}, and the
theoretical initial age was t 0 = {$-$0}.67 year
{$-$1}. The total mortality rate (Z) estimated was 2.76
year {$-$1}, with a natural mortality rate (M) and
fishing mortality rate (F) of 0.89 year {$-$1} and 1.87
year {$-$1}, respectively. The Z/k ratio was found to
be 4.11 and the exploitation rate (E) was estimated at
0.68 year {$-$1}, indicating the overexploitation of
Alosa immaculata stocks. In conclusion, this study
provides valuable insights into the population dynamics
of Pontic shad in the Danube River, Romania. The
assessments of the growth parameters, mortality rates,
and exploitation rates highlight a level of
overexploitation of Alosa immaculata stocks. These
findings underscore the importance of applying
effective fishery management strategies to ensure the
sustainability and conservation of this valuable fish
species in the Danube River ecosystem.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xu:2024:EDF,
author = "Guangming Xu and Huijie Wei and Di Peng and Lang Zhang
and Xing Lu and Qing Li and Lixue Dong and Juan Tian
and Hua Wen and Guiying Wang and Ming Jiang",
title = "Effects of Dietary Fish Meal Replaced by Cottonseed
Protein Concentrate on Growth Performance, Antioxidant
Capacity, and Liver and Intestinal Health of Juvenile
Hybrid Culter",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "127",
day = "31",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040127",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/127",
abstract = "The objective of this study was to assess the impact
of concentrated cottonseed protein (CPC) as a
substitute for fishmeal (FM) in juvenile hybrid culter
(Erythroculter ilishaeformis {[female-sign]} $ \times $
~ Ancherythroculter nigrocauda {[male-sign]}). A total
of 360 fish with an initial body weight of 10.0 {\pm}
0.5 g were randomly allocated into 12 indoor culture
tanks, with each tank containing 30 fish. Four diets
were formulated in which fish meal (FM) was replaced by
CPC at different inclusion levels: 0\% (T0), 15\%
(T15), 30\% (T30), and 45\% (T45). The corresponding
amounts of CPC included were 0, 61, 122, and 182 g/kg,
respectively. At the end of the 10-week feeding trial,
the findings revealed an inverse correlation between
the proportion of CPC replacing FM and both final body
weight (FBW) and weight growth rate (WG) in hybrid
culter. Specifically, the T30 and T45 groups exhibited
significantly lower FBW and WG compared to the T0 group
(p {$<$} 0.05). No significant differences in crude
protein and ash contents were observed among all groups
(p {$>$} 0.05). However, the crude lipid content was
highest in hybrid culter fed the T45 diet and lowest in
the T0 group (p {$<$} 0.05). There were no significant
differences in muscle amino acid composition among all
treatment groups (p {$>$} 0.05). The contents of total
protein (TP) and albumin (ALB) declined slightly as the
proportion of FM substituted by CPC increased, yet this
decline did not reach statistical significance (p {$>$}
0.05). Serum alanine aminotransferase (ALT) activity
elevated with increasing dietary CPC, and the T45 group
exhibited significantly higher ALT activity compared to
the T0 group (p {$<$} 0.05). The hepatic activities of
superoxide dismutase (SOD) and catalase (CAT) were
significantly lower in the T45 group than in the T0 and
T15 groups (p {$<$} 0.05). Furthermore, there was a
decrease in intestinal villi length with an increasing
replacement ratio of FM by CPC, and the villi length in
the T45 group exhibited a significant reduction when
compared to that in the T0 group (p {$<$} 0.05). It is
concluded that replacing 15\% FM with CPC does not
exert obviously detrimental effects on growth
performance, serum biochemical indices, intestinal
morphology, muscle amino acid profile, or antioxidant
performance of juvenile hybrid culter.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhou:2024:MEI,
author = "Jinxin Zhou and Teng Tu and Huajin Wang and Daisuke
Kitazawa",
title = "Modeling Environmental Impacts of Intensive Shrimp
Aquaculture: a Three-Dimensional Hydrodynamic Ecosystem
Approach",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "126",
day = "31",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040126",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/126",
abstract = "Already a multibillion-dollar global industry, shrimp
aquaculture, is growing all the time. The intensive
method, which is the most common method in shrimp
aquaculture, remains commercially challenged due to the
expenditures associated with environmental pollution
abatement. Although the comprehensive understanding of
this intricate aquaculture environment has been
advanced using mathematical modeling, recent attempts
to improve the model's structure have not yielded
enough results. This work upgraded the previous method
to a three-dimensional hydrodynamic ecosystem model
with the effects of shrimps being replaced by
approximation equations for the environmental
assessment of a shrimp aquaculture pond in Kyushu
District, Japan. Our approach was successful, as
demonstrated by the high consistency of the simulation
results when compared to observation data and the
previous results. Additionally, we first revealed the
impacts of stratification and confirmed the notable
daily variation in the water quality. Our case study
offers significant practical information on the
characteristics of intensive shrimp aquaculture,
implications for long-term sustainable operations, and
future research priorities on local-scale ecosystem
modeling.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Heppell:2024:CDC,
author = "Scott A. Heppell and Selina S. Heppell and N. Scarlett
Arbuckle and M. Brett Gallagher",
title = "A Cross-Decadal Change in the Fish and Crustacean
Community of \geoname{Lower Yaquina Bay, Oregon, USA}",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "125",
day = "30",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040125",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/125",
abstract = "Natural environmental change, anthropogenic
development, and inter-annual variability can affect
the ecology of estuarine fish and invertebrates.
Yaquina Bay, Oregon, a well-studied estuary, has
undergone intense development, as well as deep-draft
dredging during the latter half of the 20th century,
resulting in the alteration of ~45\% of the lower
estuary's natural shoreline. In 1967, the United States
Environmental Protection Agency (USEPA) conducted a
21-month survey of Yaquina Bay to characterize the
demersal fishes and epibenthic crustaceans that occupy
the bay. From 2003 to 2005, we conducted a 25-month
survey to replicate that work and provide a comparative
snapshot of the demersal fish and epibenthic crustacean
community in the bay. A comparison of the trawl survey
datasets reveals a 91\% decline in total catch per unit
effort (CPUE) between surveys, as well as a decline in
multiple measures of biodiversity. Furthermore, the
fishes and crustaceans of Yaquina Bay have experienced
a shift in species dominance from demersal fishes in
the late 1960s to epibenthic crustaceans in the 2000s,
marked most notably by a nine-fold increase in the
Dungeness crab CPUE. While this work does not establish
a causal relationship between changes in the demersal
communities of this West Coast estuary and human or
natural events, it does document substantial changes in
both the diversity and total abundance of animals in
that community over a three-plus decade period of
development and environmental variability. Hence, this
forms a second baseline for continued long-term
monitoring.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Fellatami:2024:AGJ,
author = "Kalysta Fellatami and Wenchao Zhang and Chi Zhang and
Shude Liu and Yongjun Tian",
title = "Age and Growth of \geoname{Japanese} Anchovy
(\bioname{Engraulis japonicus}, {Temminck} \&
{Schlegel}, 1846) in Coastal Waters around
\geoname{Shandong Peninsula, China}",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "124",
day = "30",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040124",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/124",
abstract = "Japanese anchovy (Engraulis japonicus) is a small
pelagic fish with commercial and ecological importance.
In spite of its importance, in recent years, specific
research on anchovy in the Shandong Peninsula area has
been relatively scarce and outdated. This study aims to
estimate the age and growth characteristics of anchovy
through year-round seasonal sampling from 2016 to 2017,
utilizing length--frequency and otolith microstructure
analysis. The higher coastal abundance and larger size
observed in the spring and summer suggest a potential
peak spawning period for anchovies dominated by
larger-sized individuals. Based on otolith analysis,
anchovies range in age from 0 to 4 years, with a
limited presence at age 0 and dominance in the age-1
group. The length--weight relationship (LWR) equation
shows hyper-allometric growth for each season, with a
mean relative condition factor (K n) of 1.025 {\pm}
0.005, indicating good health. Additionally, the von
Bertalanffy growth equation of can be expressed as L t
= 154.40 [1 {$-$} e {$-$0}.604 (t + 0.965) ],
suggesting a medium growth rate (K = 0.604). These
findings contribute to the understanding of anchovy age
and growth patterns, emphasizing the continuous need
for research and monitoring to support rational and
sustainable fisheries management and conservation
efforts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2024:IEP,
author = "Ping Wang and Ziling Zhu and Qinglai Xu and Yangfen
Xing and Mingyue Zhang and Jishu Zhou",
title = "Inhibitory Effect of Prickly Ash (\bioname{Zanthoxylum
bungeanum}) Seed Kernel Oil on Lipid Metabolism of
Grass Carp (\bioname{Ctenopharyngodon idellus}) in
High-Fat Diet",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "123",
day = "29",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040123",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/123",
abstract = "To investigate the effect of prickly ash (Zanthoxylum
bungeanum) seed kernel oil (PASO) on the lipid
metabolism of grass carp (Ctenopharyngodon idellus)
under a high-fat diet, PASO were added into two
lipid-level (4 g/kg and 8 g/kg) diets to form four
isonitrogenous diets: soybean oil (SO), PASO, high-fat
soybean oil (HSO), and high-fat prickly ash seed oil
(HPASO). A total of 216 healthy grass carp (9.43 {\pm}
0.82 g) were randomly divided into four groups and fed
with the four diets, respectively, for 56 days. The
result showed that the viscerosomatic index (VSI) and
the content of the crude lipid in the hepatopancreas
and muscle was significantly higher by oil levels (p
{$<$} 0.05). The linolenic acid content in the body of
the fish significantly increased in PASO and HPASO
compared to that in SO and HSO (p {$<$} 0.05). The
fatty acid composition of the hepatopancreas,
intraperitoneal fat, and muscle in four dietary groups
was significantly similar to the fatty acid composition
in the diets (p {$<$} 0.05). More significant fat
infiltration and nuclear translocation in the
hepatopancreas of fish was found in the HSO group but
was decreased in the HPASO group. The adipocyte size in
the intraperitoneal fat tissue in the PASO group was
significantly lower than that in the SO group (p {$<$}
0.05). The relative mRNA expression of the
lipogenesis-related genes ppar-{\gamma}, cebp -
{\alpha}, and srebp-1c was significantly down-regulated
in the PASO group compared with the SO group (p {$<$}
0.05), and the mRNA expression of lipolysis-related
genes ppar-{\alpha} and cpt-1 were significantly
up-regulated in the PASO group (p {$<$} 0.05). In
conclusion, dietary PASO showed the function of
reducing lipid accumulation in the fish. This reduction
might be attributed to the inhibition of the
lipogenesis-related genes and the stimulation of the
lipolysis-related genes, which were probably modulated
by the high content of linolenic acid in PASO.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mouchlianitis:2024:ESE,
author = "Foivos A. Mouchlianitis and Maria Garagouni and George
Minos and Kostas Ganias",
title = "Evaluating the Sustainability of an \geoname{Eastern
Mediterranean} Gillnet Fishery Based on the Catches of
Undersized Individuals and the Reproductive Period of
Targeted Species",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "122",
day = "29",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040122",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/122",
abstract = "The catch composition of a coastal gillnet fishery in
the Eastern Mediterranean Sea was analyzed through a
two-year experimental fishing survey. Seven fish
species occurred regularly in the hauls. Surmullet,
Mullus surmuletus, which is the most valuable demersal
fish in Greek waters and the intended target of the
gillnets in small-scale fisheries, was the most
abundant and systematically caught species. Almost all
surmullets were larger than their minimum conservation
reference size. However, three commercially exploited
species (Diplodus annularis, Pagellus acarne, and P.
erythrinus) were caught systematically as undersized
individuals. In addition, these three species were
caught mostly as immature individuals. Moreover, the
operational season of the surveyed m{\'e}tier
overlapped completely with the reproductive period of
five commercially exploited species (D. annularis, M.
barbatus, M. surmuletus, Sphyraena sphyraena, and
Trachurus trachurus). Improvements and the
establishment of additional technical measurements
should be considered for the small-scale gillnet
fisheries in the studied area to attenuate their
detrimental effects and achieve a better compromise
between sustainable exploitation of the local
multi-species fish resources and the need for an
economically sustainable practice.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{White:2024:DNF,
author = "William T. White and Andrew L. Stewart and Helen L.
O'Neill and Gavin J. P. Naylor",
title = "{Dichichthyidae}, a New Family of Deepwater Sharks
({Carcharhiniformes}) from the \geoname{Indo--West
Pacific}, with Description of a New Species",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "121",
day = "28",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040121",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/121",
abstract = "The genus Dichichthys was resurrected for five species
previously allocated to the genus Parmaturus in the
family Pentanchidae. Supraorbital crests on the
chondrocranium distinguish Dichichthys from Parmaturus
and other members of the family Pentanchidae. A new
family, Dichichthyidae, has been proposed to contain
Dichichthys. The sequence of the NADH2 mitochondrial
gene confirms the placement of Dichichthys outside of
the Pentanchidae family, as well as separate from the
Atelomycteridae and Scyliorhinidae families.
Dichichthys albimarginatus was described using a
holotype collected off the coast of New Caledonia. A
second juvenile specimen collected off the coast of
Papua New Guinea was tentatively assigned as D. cf.
albimarginatus. Dichichthys bigus is known from the
holotype collected in the Coral Sea off the coast of
Queensland, Australia. A new, parasite-afflicted
underwater observation was reported further north of
Queensland. The type species Dichichthys
melanobranchus, previously only known from juvenile
specimens, was redescribed based on adult specimens.
Dichichthys nigripalatum is known from the holotype
collected off Sumbawa, Indonesia, and a tentatively
identified photo record from West Java. Dichichthys
satoi n. sp. is described from the West Norfolk Ridge
and off the North Island of New Zealand. Members of the
genus Dichichthys have unique curved egg cases which
have pliable ridges made up of numerous fibres and long
coiled tendrils on the posterior end.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2024:MCE,
author = "Xiaotian Zhang and Yuxia Wu and Yang Zhang and Jin
Zhang and Kunci Chen and Haiyang Liu and Qing Luo and
Shuzhan Fei and Jian Zhao and Mi Ou",
title = "Molecular Characteristics, Expression Patterns, and
Response of Insulin-like Growth Factors Gene Induced by
Sex Steroid Hormones in Blotched Snakehead
(\bioname{Channa maculata})",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "120",
day = "28",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040120",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/120",
abstract = "Insulin-like growth factors (IGFs) play central roles
in the growth and development of vertebrates. Blotched
snakehead (Channa maculata), an economically
significant fish, exhibits obvious sexual dimorphism
and achieves sexual maturity in one year. However, the
role of IGFs in C. maculata remains unknown. Three IGF
genes were identified in C. maculata, designated as
CmIGF1-1, CmIGF1-2, and CmIGF2. The cDNA sequences of
these genes are 1184, 655, and 695 bp, encoding
putative proteins of 168, 131, and 215 amino acids,
respectively, and all three proteins contain a
conserved IGF domain. Quantitative real-time PCR (qPCR)
revealed the predominant expression of CmIGFs in the
liver of adult fish, with higher expression levels
observed in males. Notably, CmIGF1-1, CmIGF1-2, and
CmIGF2 displayed analogous expression profiles in the
liver across various developmental stages, peaking at
365 days after hatching (dah). Subsequently, 600
individuals at 75 dah, at an early developmental stage,
were randomly divided equally into six groups and
reared in aerated 2 m $ \times $ 2 m $ \times $ 2 m
cement ponds at 26.0 {\pm} 1.0 {$^\circ $}C. Following
a one-week acclimatization period, fish without
observed abnormalities were intraperitoneally injected
with either 17{\alpha}-ethynylestradiol (EE 2) or
17{\alpha}-methyltestosterone (MT) at a dose of 10
{\mu}g/g body weight. Three groups underwent short-term
hormone treatment, and the remaining three groups
underwent long-term hormone treatment, which included
five injections at two-week intervals over ten weeks.
The analysis of CmIGFs expression levels in the liver
under different hormone treatments revealed that EE 2
suppressed the expression of CmIGF1-1 and CmIGF1-2
while promoting CmIGF2 expression. In females, MT
up-regulated the expression of CmIGF1-1 and CmIGF2 in a
time-dependent manner, but consistently inhibited
CmIGF2 expression. In males, MT promoted the expression
of CmIGFs in a time-dependent manner, reaching peak
levels for CmIGF1-1, CmIGF1-2, and CmIGF2 after 8, 10,
and 2 weeks of injection, respectively. Additionally,
CmIGF1 and CmIGF2 might exhibit a complementary
relationship, with a compensatory increase in CmIGF2
expression in response to low CmIGF1 concentration.
These findings highlight the potential key role of IGFs
upon growth and their regulation by sex steroid
hormones in C. maculata, providing a crucial foundation
for future research aimed at elucidating the molecular
mechanisms underlying the growth dimorphism between
female and male blotched snakeheads.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2024:VPE,
author = "Jia Chen and Haiying Liang and Danqing Lin and Jialu
Zhang and Dong Li and Kun Ye and Wenfei Lu and Kai
Liu",
title = "Vocalization Pattern and Echolocation Signal
Characteristics of \geoname{Yangtze} Finless Porpoise
(\bioname{Neophocaena asiaeorientalis asiaeorientalis})
in Captivity",
journal = j-FISHES,
volume = "9",
number = "4",
pages = "119",
day = "28",
month = mar,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9040119",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/4/119",
abstract = "The Yangtze finless porpoise (Neophocaena
asiaeorientalis asiaeorientalis, YFP) possesses the
ability to detect distance through echolocation
signals, and its sonar signal signature is adjusted to
detect different targets. In order to understand the
vocal characteristics of YFPs in different behavioral
states and their differential performance, we recorded
the vocal activities of YFPs in captivity during
free-swimming, feeding, and nighttime resting and
quantified their signal characteristic parameters for
statistical analysis and comparison. The results showed
that the number of vocalizations of the YFPs in the
daytime free-swimming state was lower than that in the
feeding and nighttime resting states, and the
echolocation signals emitted in these three states
showed significant differences in the {$-$10} dB
duration, {$-$3} dB bandwidth, {$-$10} dB bandwidth,
and root-mean-square (RMS) bandwidth. Analysis of the
resolution of the echolocation signals of the YFPs
using the ambiguity function indicated that their
distance resolution could reach the millimeter level.
These results indicate that the echolocation signal
characteristics of YFPs present diurnal differences and
that they can be adjusted with changes in their
detection targets. The results of this study can
provide certain scientific references and foundations
for the studies of tooth whale behavioral acoustics,
and provide relevant scientific guidance for the
conservation and management of YFPs.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2024:ICP,
author = "Xingbing Wu and Baolin Cheng and Mingyang Xue and Nan
Jiang and Xuemei Li and Xiaona Hu and Xiaoli Li and
Tingbing Zhu and Yongjiu Zhu and Yong Zhou",
title = "Isolation, Characterization, and Pathogenicity of an
\bioname{Aeromonas veronii} Strain Causing Disease in
\bioname{Rhinogobio ventralis}",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "188",
day = "18",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050188",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/188",
abstract = "Rhinogobio ventralis is a rare fish found in the
Yangtze River in China and has significant ecological
and economic value. In this study, a bacterial strain
(RV-JZ01) was isolated from the livers of diseased R.
ventralis. This isolate was identified as Aeromonas
veronii based on its morphology, biochemical features
and 16S rDNA phylogenetic analysis. The artificial
infection of healthy R. ventralis (16 {\pm} 2 cm) with
RV-JZ01 resulted in the manifestation of clinical
symptoms, in accordance with those of naturally
infected fish. The 50\% lethal dose (LD 50) of RV-JZ01
for R. ventralis was 6.3 $ \times $ 10 6 CFU/mL.
Histopathological examination revealed various
pathological changes in the diseased fish, including
intestinal villus swelling and rupture, hepatocyte
vacuolization, renal tubular cell nuclear enlargement
and pyknosis, and myocardial fiber fracture and
atrophy. RV-JZ01 infection significantly reduced the
gut flora diversity of R. ventralis, with the relative
abundances of Firmicutes and Fusobacteria increasing,
and those of the Proteobacteria and Bacteroidetes
decreasing. The abundance of Lactobacillus and
Streptococcus dramatically increased, and the abundance
of Clostridium and Escherichia reduced in the
intestinal microbiota of R. ventralis infected with
RV-JZ01. Antibiotic sensitivity testing revealed that
RV-JZ01 was highly susceptible to 12 antimicrobials,
including erythromycin, cefalexin, norfloxacin,
furazolidone, sulfonamides, enrofloxacin, doxycycline,
piperacillin, florfenicol, gentamicin, and lincomycin.
These results contribute to the understanding of
pathological alterations in R. ventralis following A.
veronii infection, offering valuable data to support
the implementation of disease treatment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mullowney:2024:MMI,
author = "Darrell Mullowney and Krista Baker and Julia Pantin
and William Coffey and Kyle Lefort and Katie
Morrissey",
title = "Merits of Multi-Indicator Precautionary Approach
Management in a Male-Only Crab Fishery",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "187",
day = "18",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050187",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/187",
abstract = "Snow crab replaced groundfish fisheries as the primary
focus of the Newfoundland and Labrador (NL) fishing
industry in the mid-1990s. Over the past three decades,
management philosophies in this male-only fishery have
shifted from promoting industry expansion to promoting
industry rationalization to implementing Precautionary
Approach (PA) management. Until the mid-2010s, there
were regionalized management strategies characterized
by higher exploitation rates in northern than southern
sub-stock units along the NL marine shelves. However,
in the late 2010s, exploitation rates were permitted to
elevate to high levels across the entire stock range in
association with a large resource decline, and evidence
of biological harm through fishing emerged in
chronically depleted areas. In 2019, when stock and
fishery productivity were near historical lows, a
multi-indicator PA system was informally introduced
into the management of the resource. This event
coincided with anticipated improvements in stock and
fishery performance. This paper examines the extent to
which the multi-indicator PA management system, focused
on the promotion of both biological protection and
maximization of fishing efficiency, may have
contributed to recent improvements in stock and fishery
status. A suite of indicators from areas implementing
PA guidance into management in 2019 prior to the formal
adoption of the PA system in 2023 suggest the system
has been highly beneficial in promoting rapid recovery
and improving stock and fishery performance metrics to
levels matching or exceeding historical levels under
similar conditions. We discuss the capacity of the
system to better safeguard biological aspects of
resource and fisheries productivity moving forward
under a scenario of an expectant resource decline.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gomes:2024:OFF,
author = "Maria Fernanda S. Gomes and Paulo Henrique R. Aride
and Maiko Willas S. Ribeiro and Cristiane C.
Guimar{\~a}es and Tiago C. N{\'o}brega and Rayana M.
Paix{\~a}o and Adriene N{\'u}zia A. Santos and Adriano
Teixeira de Oliveira",
title = "Occurrence of Four Freshwater Stingrays
({Chondrichthyes: Potamotrygoninae}) in the
\geoname{Uatum{\~a} River Basin, Amazon Region}: a
Field Study",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "186",
day = "17",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050186",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/186",
abstract = "The Amazon region has the largest diversity of
freshwater stingrays; however, there are still places
where information about this group is scarce. The
present work aims to record the occurrence of
freshwater stingrays in the Uatum{\~a} river basin,
Amazonas, Brazil. For this, collections were carried
out in three municipalities bathed by the Uatum{\~a}
River, Presidente Figueiredo, Itapiranga, and S{\~a}o
Sebasti{\~a}o do Uatum{\~a}. The rays were collected
through fishing using a hand net and, after capture,
were identified through the pattern of the dorsal and
ventral color of the disc, the absence or presence of
the labial groove, and the organization of the row of
spines on the tail, and were also measured, weighed,
and sexed. In total, 69 specimens of Potamotrygonines
were captured, 59 of which were from the genus
Potamotrygon and ten individuals from the genus
Paratrygon. The present study provides preliminary
information on the characteristics of freshwater
stingray species that may occur in the Uatum{\~a}
River. Furthermore, new research is necessary to
improve the identification of specimens to determine
the diversity and elucidate aspects related to the
biology of elasmobranchs in the Uatum{\~a} River Basin,
Brazil.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Frank:2024:LIP,
author = "Colin Frank and Shannon Bayse and Rioghnach Steiner
and Pierre-Paul Bitton",
title = "Light Intensity of Phosphorescent-Netting Pots and
Determining Their Visibility to Snow Crab
(\bioname{Chionoecetes opilio}) Using Visual Modeling
Techniques",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "185",
day = "17",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050185",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/185",
abstract = "This study explores the visibility of
phosphorescent-netting pots to snow crab (Chionoecetes
opilio) using visual modeling techniques. Light emitted
from such pots increases catch per unit effort, yet
little is understood about the factors driving these
higher catch rates. In this study, we measure pot light
emission and snow crab visual acuity. Combining these
data with estimates obtained in the literature for
other biotic and abiotic factors, we model snow crab
vision in relation to the pots. Utilizing these factors
and environmental conditions, we derive a contrast
ratio between the pot light and the ambient light.
Findings reveal that the visibility of pot lights at
200-m depth depends primarily on solar angle (time of
day) and time elapsed post-deployment. Additional
factors influencing the vision of the pots include
water column quality and benthic boundary layer
turbidity. This study is the first to model the visual
ecology of snow crab and the first to estimate snow
crab visual acuity. These insights into snow crab
visual ecology can potentially enhance fishing
techniques, promote catch efficiency and
sustainability, and help provide a path forward for
visual ecology research in the fisheries science
field.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:CAE,
author = "Dongge Liu and Hao Yang and Shuisheng Li and Hai Huang
and Guangli Li and Huapu Chen",
title = "Comparative Analysis of Enzymatic Activities and
Transcriptional Profiles of Various Hepatic Enzymes
between Male and Female Yellowfin Tuna
(\geoname{Thunnus albacares})",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "184",
day = "17",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050184",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/184",
abstract = "Yellowfin tuna (Thunnus albacares) is a valuable
pelagic migratory fish with potential for aquaculture.
Despite this, there is limited understanding of the
biological and physiological characteristics of this
species, particularly regarding sex differences in
growth performance. The liver, a crucial organ for
digestion and metabolism, plays a significant role in
regulating fish growth. This study aimed to compare
liver enzyme activities and transcriptome profiles
between female and male yellowfin tuna to uncover the
molecular mechanisms underlying difference between the
sexes. The results revealed that female yellowfin tuna
exhibited higher amylase and lipid metabolism enzyme
activities, while male yellowfin tuna showed higher
glucose-6-phosphate dehydrogenase and antioxidant
enzyme activities. Additionally, through Illumina
sequencing technology, the study generated 37.74 Gb of
clean data and identified 36,482 unique genes
(UniGenes) in the liver transcriptome. A total of 2542
differentially expressed genes were found, with
enriched Gene Ontology terms and pathways related to
metabolic processes, particularly lipid metabolism and
transport. These findings suggest that female yellowfin
tuna have superior digestive enzyme activities and
lipid metabolism, while male yellowfin tuna excel in
sugar metabolism, ATP production, and antioxidant
defense. This study provides valuable insights into sex
differences in yellowfin tuna and could aid in
advancing full-cycle aquaculture practices for this
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Suhnel:2024:SDD,
author = "Simone S{\"u}hnel and Francisco Jos{\'e}
Lagreze-Squella and Gabriel Nandi Corr{\^e}a and
Jaqueline Ara{\'u}jo and Glauber de Souza and Jo{\~a}o
Paulo Ramos Ferreira and Francisco Carlos da Silva and
Carlos Henrique Ara{\'u}jo de Miranda Gomes and Claudio
Manoel Rodrigues de Melo",
title = "Stocking Density and Diet of Two Oyster
(\bioname{Crassostrea gasar} and \bioname{Crassostrea
gigas}) Seeds in Fluidized Bed Bottle Nursery System",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "183",
day = "17",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050183",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/183",
abstract = "Crassostrea is the most farmed oyster genus worldwide
and has significant economic and social impacts with
environmental benefits. Hatchery oyster seed production
is a highly costly phase, and a fluidized nursery
system can help reduce this cost and reduce seed
production time. The present study evaluated the
survival and growth of two oyster species (Crassostrea
gasar and Crassostrea gigas) in a fluidized bed bottle
nursery system. With C. gasar, two experiments were
performed; one tested three stocking densities and the
other three bialgae diets. With C. gigas, one
experiment with a bialgae and monoalgae in an initial
bottle occupation of 8.8\% produced more seeds per
bottle, but an initial bottle occupation of 2.2\%
produced bigger seeds. Also, the experiment with C.
gasar and with C. gigas tested diets did not affect
seed survival, but the diets with bialgae I. galbana
and N. oculate promoted more seed growth. The fluidized
bed bottle nursery system developed for this study was
adequate for the seeds of the oysters C. gasar and C.
gigas in the nursery phase.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Gao:2024:CGB,
author = "Jinwei Gao and Hao Wu and Xing Tian and Jiayu Wu and
Min Xie and Zhenzhen Xiong and Dongsheng Ou and
Zhonggui Xie and Rui Song",
title = "Calmodulin Gene of Blunt Snout Bream
(\bioname{Megalobrama amblycephala}): Molecular
Characterization and Differential Expression after
\bioname{Aeromonas hydrophila} and Cadmium Challenges",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "182",
day = "16",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050182",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/182",
abstract = "Calmodulin (Calm), a crucial Ca 2+ sensor, plays an
important role in calcium-dependent signal transduction
cascades. However, the expression and the relevance of
Calm in stress and immune response have not been
characterized in Megalobrama amblycephala. In this
study, we identified the full-length cDNA of Calm
(termed MaCalm) in blunt snout bream M. amblycephala,
and analyzed MaCalm expression patterns in response to
cadmium and Aeromonas hydrophila challenges. MaCalm was
1603 bp long, including a 5{\prime}-terminal
untranslated region (UTR) of 97 bp, a
3{\prime}-terminal UTR of 1056 bp and an open reading
frame (ORF) of 450 bp encoding a polypeptide of 149
amino acids with a calculated molecular weight (MW) of
16.84 kDa and an isoelectric point (pI) of 4.09.
Usually, MaCalm contains four conservative EF hand
motifs. The phylogenetic tree analysis indicated that
the nucleotide sequence of MaCalm specifically
clustered with Ctenopharyngodon idella with high
identity (98.33\%). Tissue distribution analysis
demonstrated that the ubiquitous expression of MaCalm
mRNA was found in all tested tissues, with the highest
expression in the brain and the lowest expression in
muscle. MaCalm showed significant upregulation at 14 d
and 28 d post exposure to varying concentrations of
cadmium in the liver; HSP70 transcripts in the liver
significantly upregulated at 14 d post exposure to
different concentrations of cadmium. Moreover, in
response to the A. hydrophila challenge in vivo, MaCalm
transcripts in the liver first increased and then
decreased, but MaCalm transcripts in the kidney
declined gradually with prolonged infection. After the
A. hydrophila challenge, the expression level of HSP70
was significantly downregulated at 24 h in the liver
and its expression level was notably downregulated at
12 h and at 24 h in the kidney. Collectively, our
results suggest that MaCalm possesses vital roles in
stress and immune response in M. amblycephala.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Riepe:2024:TTL,
author = "Tawni B. Riepe and Zachary E. Hooley-Underwood and
Megan Johnson",
title = "Thermal Tolerance of Larval Flannelmouth Sucker
Catostomus latipinnis Acclimated to Three
Temperatures",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "181",
day = "16",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050181",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/181",
abstract = "As water temperatures rise in streams due to global
temperature variations, dams, and increased water
usage, native fish species face uncertain futures. Our
study defines the thermal limits of flannelmouth sucker
larvae. By raising sucker eggs at three acclimation
temperatures (11 {$^\circ $}C, 16 {$^\circ $}C, and 22
{$^\circ $}C), we defined ideal conditions for larval
survival and the temperature tolerance range using
critical thermal maximum (CTMax) and minimum (CTMin)
trials. Larvae survived best at 16 {$^\circ $}C. Within
our three acclimation temperatures, our data suggest
that larvae can survive static temperatures between 6.9
{$^\circ $}C and 26.4 {$^\circ $}C. Beyond an upper
temperature of 34.8 {$^\circ $}C and a lower
temperature of 6.3 {$^\circ $}C, these fish may fail to
adapt. While flannelmouth suckers withstand high
temperatures, even small temperature decreases prove
detrimental. By defining the temperature limits of the
flannelmouth sucker, we can make informed management
decisions to preserve the populations of this desert
fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chai:2024:AED,
author = "Yuqiao Chai and Shuhao Sun and Yingdong Li",
title = "Assessing the Effects of Dietary Tea Polyphenols on
the Gut Microbiota of Loaches (\bioname{Paramisgurnus
dabryanus}) under Chronic Ammonia Nitrogen Stress",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "180",
day = "15",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050180",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/180",
abstract = "This study examined the impact of tea polyphenols
(TPs) on the intestinal flora of loaches
(\bioname{Paramisgurnus dabryanus}) under chronic
ammonia nitrogen stress using high-throughput
sequencing. Two groups of 600 loaches were studied over
one month, and they were separated into a control group
and tea polyphenol group. Alpha and beta diversity
analyses showed diverse bacterial communities, with
significant differences in the abundance and uniformity
observed initially but not between sampling time
points. Cluster analyses revealed distinct differences
in microbial communities between groups. A predictive
function analysis indicated enrichment in pathways
related to amino acid and nucleotide biosynthesis.
These findings offer initial insights into how tea
polyphenols may affect intestinal microbial communities
in loaches under ammonia nitrogen stress.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ju:2024:IAM,
author = "Pei-Mo Ju and Shi-Wei Ma and Yi-Yang Li and Shi-Fan
Zhang and Jun Li and Guang-Zhou Zhou",
title = "Investigation of the Antiviral Mechanism of Curcumin
Analog {EF-24} against \bioname{Siniperca cachuatsi}
Rhabdovirus",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "179",
day = "15",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050179",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/179",
abstract = "Siniperca chuatsi rhabdovirus (SCRV) is a major strain
of viral fish virus resulting in multiple transmissions
and devastating damage in aquaculture. Currently, there
are no available approved therapeutics. In this study,
we screened and identified a novel curcumin analog
(EF-24) for evaluating its in vitro anti-SCRV
properties and potential molecular mechanisms. Present
results demonstrated that EF-24 could strongly delay
the occurrence of cytopathic effects (CPEs) in
epithelioma papulosum cyprinid cells (EPCs) and inhibit
SCRV replication and viral nucleoprotein expression in
the early stages of infection by the time-of-addition
assay. Furthermore, flow cytometry analysis after
Annexin V-FITC/PI double staining and
immunofluorescence microscopy observation after JC-1
incubation showed that EF-24 downregulated cell
mitochondrial apoptosis induced by SCRV. The enzymatic
activities of caspase-3 and caspase-9 were also reduced
after EF-24 treatment, indicating that EF-24 may
protect cells from SCRV infection by decreasing
mitochondrial intrinsic apoptosis in infected cells.
Collectively, we demonstrated for the first time that
the curcumin analog EF-24 possesses antiviral ability
against SCRV, suggesting its potential for effective
control of fish rhabdovirus spreading.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2024:EGI,
author = "Chang'an Wang and Fangyuan Li and Di Wang and Shaoxia
Lu and Shicheng Han and Wei Gu and Haibo Jiang and
Zhuang Li and Hongbai Liu",
title = "Enhancing Growth and Intestinal Health in Triploid
Rainbow Trout Fed a Low-Fish-Meal Diet through
Supplementation with \bioname{Clostridium butyricum}",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "178",
day = "14",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050178",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/178",
abstract = "This study evaluates the effects of dietary
Clostridium butyricum on growth performance and
intestinal health in triploid rainbow trout
(\bioname{Oncorhynchus mykiss}). Administered in a
12-week trial, five isonitrogenous and isolipidic feeds
contained different C. butyricum levels [G1 (0), G2
(1.6 $ \times $ 10 6 CFU/g), G3 (1.2 $ \times $ 10 7
CFU/g), G4 (1.1 $ \times $ 10 8 CFU/g), and G5 (1.3 $
\times $ 10 9 CFU/g)]. Significant enhancements in
growth performance, including improved feed conversion
ratios and specific growth rates in the G4 group, were
indicative of enhanced nutrient utilization,
corroborated by optimal digestive enzyme activity
levels. Antioxidant capabilities were also enhanced in
the G4 group, indicated by increased serum superoxide
dismutase and catalase activities, along with a
significant decrease in malondialdehyde levels. Gut
microbiota analysis indicated a probiotic
concentration-dependent modulation of microbial
communities, with a marked enrichment in beneficial
bacterial phyla like Firmicutes in the G4 group. This
microbial shift correlated with significant
downregulations in immune-related gene expressions,
including interleukins (IL-1{\beta}, IL-8),
NF-{\kappa}B, MyD88, and TNF-{\alpha}, highlighting an
activated immune response. Correspondingly, serum
lysozyme and immunoglobulin M contents were
significantly elevated in the G4 group. Challenge tests
with Aeromonas salmonicida exhibited the higher disease
resistance of fish fed the G4 diet. In conclusion, the
study shows the potential of dietary C. butyricum,
especially at 1.1 $ \times $ 10 8 CFU/g, in enhancing
the growth, health, and disease resistance of triploid
rainbow trout through modulating gut microflora and
stimulating immune responses.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wu:2024:TOS,
author = "Song Wu and Peng Wang and Ying Zhang and Li Huang and
Qirui Hao and Lei Gao and Dongli Qin and Xiaoli Huang",
title = "Toxicity, Oxidative Stress, and Tissue Distribution of
Butachlor in the Juvenile \geoname{Chinese} Mitten Crab
(\bioname{Eriocheir sinensis})",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "177",
day = "13",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050177",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/177",
abstract = "The Chinese mitten crab (Eriocheir sinensis) is one of
the most commercially important crustacean species in
China. The aim of this study was to characterize the
toxic effects of butachlor (an herbicide of the
acetanilide class) on juvenile E. sinensis crabs. The
lethal effects and the acute toxicity of butachlor on
juvenile E. sinensis specimens were assessed through a
semi-static in vitro experiment. We determined the
activities of superoxide dismutase (SOD) and catalase
(CAT) as well as the levels of glutathione (GSH) and
malondialdehyde (MDA) in the gills and the
hepatopancreas of the juvenile crabs, at different time
points over a 14-day short-term exposure to butachlor.
Moreover, we measured the residual levels of butachlor
in three different tissues (gills, hepatopancreas, and
muscles) of the juvenile crabs over a longer period.
Our findings revealed that butachlor is highly toxic
for juvenile E. sinensis crabs. In fact, the median
lethal concentration (LC 50) values of butachlor at 24,
48, 72, and 96 h were found to be 4.22, 1.84, 0.34, and
0.14 mg/L, respectively, while the safe concentration
was 0.014 mg/L. The antioxidant defense ability of the
juvenile E. sinensis crabs against butachlor was
induced after exposure to the herbicide at a
concentration of 0.01 mg/L. After 14 days of exposure
to butachlor at 0.04 and 0.16 mg/L, both SOD and CAT
were found to be significantly inhibited (p {$<$}
0.05), the GSH levels were found to be significantly
decreased (p {$<$} 0.05) and the MDA levels were
identified as significantly increased (p {$<$} 0.05).
Moreover, after 14 days of exposure to butachlor at
0.16 mg/L, the activities of SOD and CAT as well as the
content of GSH in the hepatopancreas were found to be
significantly decreased (p {$<$} 0.05). Our results
revealed that a high concentration of butachlor was
capable of inducing oxidative stress and damage in
juvenile E. sinensis crabs. The maximal residual value
of butachlor was obtained in the gills, with a content
of 4.56 {\mu}g/kg. Butachlor was not detected after 24
days in the aforementioned three tissues of the
juvenile crabs, thereby indicating that it was
effectively metabolized.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Qi:2024:EDS,
author = "Changle Qi and Qiyou Xu and Jianhua Ming and Fei Song
and Chuanpeng Zhou",
title = "Effect of Dietary Supplementation on the Growth and
Immunity of Fish and Shellfish",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "176",
day = "13",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050176",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/176",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ruan:2024:EIA,
author = "Zhuohao Ruan and Ruilin Xie and Yifu Li and Yuanyuan
Luo and Zufeng Weng and Wensheng Liu",
title = "Effect of an \bioname{Ipomoea aquatica} Floating Raft
on the Water Quality, Antioxidant System, Non-Specific
Immune Responses, and Microbial Diversity of
\bioname{Penaeus vannamei} in an Aquaculture System",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "175",
day = "12",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050175",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/175",
abstract = "Pacific white shrimp (Penaeus vannamei) is one of the
main shrimp species cultivated around the world.
Despite its high yields and easy handling, water
pollution from intensive shrimp cultivation remains a
serious problem in China. In this study, a compound
aquaculture model of P. vannamei and water spinach (
Ipomoea aquatica) was used to investigate the effect of
a water spinach floating raft on water quality,
antioxidants, non-specific immune response, growth
performance, and microbial diversity. The experimental
design of this study consisted of two groups with three
replicates for each, i.e., control group: aquatic
monoculture (AM) system with only P. vannamei;
treatment group: P. vannamei - I. aquatica raft
aquaponics (AP) system with a 50\% cover ratio with a
water spinach floating raft. The experiment lasted for
seven weeks. The results show that the concentrations
of total phosphorus (TP), total nitrogen (TN), nitrate
nitrogen (NO 3 {$-$} -N), ammonia nitrogen (NH 4 + -N),
nitrite nitrogen (NO 2 {$-$} -N), and active phosphorus
(AP) in the AM group were higher than those in the AP
group at different sampling times. The water quality
index of the AP group was better than that of the AM
group, indicating that water spinach can remove the
nutrients from aquaculture water bodies. The average
daily gain and survival rate of shrimp in the AP group
were higher than those in the AM group. The total
antioxidant capacity (T-AOC), catalase (CAT),
superoxide dismutase (SOD), malondialdehyde (MDA), and
acid phosphatase (ACP) in the AP group were better than
those in the AM group. The Shannon--Wiener and Simpson
indices of the gut, water, and sediment of the AP
system were significantly higher than those in the AM
system, which implied a higher abundance of
microorganisms in the AP system. These results
demonstrate that the application of a water spinach
floating raft in aquaponics can not only improve the
water quality, but also improve the growth performance,
antioxidant system, and non-specific immune responses
of Pacific white shrimp, while increasing the abundance
of microorganisms in the aquaculture system and
improving the ecological benefits in terms of the
expenditure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2024:ETS,
author = "Lei Wang and Xiaowen Long and Yaopeng Li and Yong
Zhang and Weihong Sun and Xugan Wu",
title = "Effects of Three Sources of Astaxanthin on the Growth,
Coloration, and Antioxidant Capacity of Rainbow Trout
(\bioname{Oncorhynchus mykiss}) during Long-Term
Feeding",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "174",
day = "10",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050174",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/174",
abstract = "Astaxanthin is an important pigment for the rainbow
trout Oncorhynchus mykiss. This study was conducted to
investigate the effects of different sources of dietary
astaxanthin on the growth, coloration, and antioxidant
capacity of the commercial-sized O. mykiss during
long-term feeding. Haematococcus pluvialis (HP), yeast
Phaffia rhodozyma (PR), and synthetic astaxanthin (SA)
were added to the basic feed (no astaxanthin, NA) to
prepare the isonitrogenous and isolipidic experimental
diets; the actual astaxanthin content values in the
diets were 31.25, 32.96, and 31.50 mg/kg, respectively.
Eighteen hundred O. mykiss, averaging 670 {\pm} 20 g,
were randomly divided into four groups and then fed
with the experimental diet for four months. Dietary
supplementation of P. rhodozyma and synthetic
astaxanthin had no significant effects on the growth
and tissue indexes of O. mykiss. In contrast, dietary
supplementation with astaxanthin from H. pluvialis
significantly increased the weight gain rate after four
months of feeding. The fillet lightness of O. mykiss in
the PR and SA was statistically lower than that in the
NA and HP; the redness and astaxanthin content of
fillet in the HP, PR, and SA groups were statistically
higher than those in the NA. The total antioxidant
capacity of the liver and serum in the HP was
statistically higher than that in other diet groups,
and a higher liver total superoxide dismutase activity
was detected in the HP compared with the PR. Dietary
supplementation of astaxanthin significantly increased
the glutathione peroxidase activity in the liver and
serum, and the highest serum glutathione peroxidase
activity was detected in the HP, while dietary
astaxanthin significantly decreased the malondialdehyde
content in the liver and serum. Dietary supplementation
of PR significantly increased the fillet ash content,
while the highest fillet total lipid content was
detected in the HP. Dietary astaxanthin significantly
improved fillet redness and antioxidant capacity, among
which H. pluvialis astaxanthin has greater effects on
improving weight gain, antioxidant capacity, and fillet
total lipid content.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Rodriguez-Garcia:2024:CSZ,
author = "Carlos Rodr{\'\i}guez-Garc{\'\i}a and Ana
Toro-Podadera and Jesica Sarmiento-Carbajal and
Remedios Cabrera-Castro",
title = "Coexisting in the Surf Zone: Age and Feeding Habits of
the Spotted Seabass (\bioname{Dicentrarchus punctatus})
and \geoname{European} Seabass (\bioname{Dicentrarchus
labrax}) on the \geoname{Gulf of C{\'a}diz} Beaches
(\geoname{Southwest Iberian Peninsula})",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "173",
day = "9",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050173",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/173",
abstract = "Various commercial fish species utilize different
coastal habitats as nurseries in their juvenile stages,
with surf zones being particularly crucial due to the
protection and abundant food resources they offer.
Among the species that rely on these areas are the
spotted seabass (\bioname{Dicentrarchus punctatus}) and
the European seabass (Dicentrarchus labrax). This study
aimed to investigate the age and feeding habits of surf
zone specimens of these species and explore their
environmental adaptations. The average size for D.
punctatus was 16.94 {\pm} 4.05 cm, and for D. labrax,
it was 23.23 {\pm} 6.30 cm. The length--weight
relationship for D. punctatus was TW = 0.013*TL 2.885
(parameter a: 95\% CI: 0.012--0.014; parameter b: 95\%
CI: 2.854--2.917), and for D. labrax, it was TW =
0.008*TL 3.095 (parameter a: 95\% CI: 0.006--0.009;
parameter b: 95\% CI: 3.040--3.151). In both D.
punctatus (79.20\%) and D. labrax (75.92\%), the
predominant age classes were 1+ and 2+. Significant
variations in age class abundance were observed in D.
punctatus based on the time of day, lunar phase, and
season, while D. labrax showed variations only by
season. A diet analysis revealed that both species
primarily consume mysids and fish, with D. punctatus
showing dietary variations related to the time of day,
lunar phase, and season, and D. labrax showing
variations with the time of day and season.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lin:2024:CCF,
author = "Jinji Lin and Ziqi Yu and Yang Leng and Jiexiong Zhu
and Feifei Yu and Yishan Lu and Jiayu Chen and Wenhao
He and Yixin Zhang and Yaoshen Wen",
title = "Cloning, Characterization and Functional Analysis of
Caspase 8-like Gene in Apoptosis of Crassostrea
hongkongensis Response to Hyper-Salinity Stress",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "172",
day = "9",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050172",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/172",
abstract = "Caspase-8, a member of the caspase family, is an
initiating caspase and plays a crucial role in
apoptosis. In this study, the full-length cDNA of
caspase8-like (CASP8-like) was isolated from
Crassostrea hongkongensis (C. hongkongensis) by
RACE-PCR. ChCASP8-like contained a 1599-bp open reading
frame (ORF) encoding 533 amino acids with two conserved
death effector domains (DEDs) and a cysteine aspartase
cysteine structural domain (CASc). Amino acid sequence
comparison showed that ChCASP8-like shared the highest
identity (85.4\%) with CASP8-like of C. angulata. The
tissue expression profile showed that ChCASP8-like was
constitutively expressed in gills, hepatopancreas,
mantle, adductor muscle, hemocytes and gonads, and was
significantly upregulated in hemocytes, hepatopancreas
and gills under hyper-salinity stress. The
apoptosis-related genes, including ATR, CHK1, BCL-XL,
CASP8-like, CASP9 and CASP3, were significantly
activated by hyper-salinity stress, but were remarkably
inhibited by ChCASP8-like silencing. The caspase 8
activity was increased by 1.7-fold after hyper-salinity
stress, and was inhibited by 9.4\% by ChCASP8-like
silencing. Moreover, ChCASP8-like silencing clearly
alleviated the apoptosis resulting from hyper-salinity
stress. These results collectively demonstrated that
ChCASP8-like played a crucial role in inducing
apoptosis against hyper-salinity stress.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Madia:2024:RAF,
author = "Manfredi Madia and Massimiliano Bottaro and Tiziana
Cillari and Andrea {Li Vorsi} and Luca Castriota and
Maria Rita Amico and Sergio Bizzarri and Teresa Maggio
and Manuela Falautano and Michele Gristina and Ilaria
{Di Lauro} and Floriana Trova and Patrizia Perzia and
Roberto D'Ambra and Enrico Casola and Mauro Sinopoli",
title = "Reducing Artisanal Fishery Impact on Marine Community:
New Data from Comparison of Innovative and Traditional
Gear",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "171",
day = "8",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050171",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/171",
abstract = "Fishery resources overexploitation, together with
bycatch and discards, have an impact on marine
ecosystems. The adoption of technologically innovative
gears is a possible solution to reduce the discard and
to enhance the sustainability of fishery, mainly in
artisanal fisheries that represent about 80\% of the EU
Mediterranean fleet. In the perspective of fishery
sustainability, it is necessary to study and test
alternative gears to the traditional ones, also with
collaboration of fishers. In the present study, results
of experimental fishing activities carried out in two
Mediterranean areas with traditional and innovative
gears of small-scale fishing are reported. Thirty-four
hauls were carried out to compare the catch of two
types of trammel nets: a traditional one and
experimental guarding net. Additionally, 12 hauls were
carried out to compare collapsible pots and traditional
pots. No significant differences were recorded between
trammel nets in terms of commercial catch and discard.
However, interesting differences in discard composition
were recorded, with higher Elasmobranchs presence in
trammel nets, including species assessed as critically
endangered and vulnerable. The use of trapula pots in
place of traditional ones showed a lower discard of
specific resources and an advantage for fishers in
terms of space occupied on board.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhong:2024:ERI,
author = "Hua Zhong and Xinyi Yao and Haihui Tu and Zhenglong
Xia and Miaoying Cai and Qiang Sheng and Shaokui Yi and
Guoliang Yang and Qiongying Tang",
title = "Effects of {RNA} Interference with Acetyl-{CoA}
Carboxylase Gene on Expression of Fatty Acid
Metabolism-Related Genes in \bioname{Macrobrachium
rosenbergii} under Cold Stress",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "170",
day = "8",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050170",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/170",
abstract = "Macrobrachium rosenbergii is a warm water species, and
low temperature is a limiting factor for its growth and
survival. In order to explore the role of the
acetyl-CoA-carboxylase (ACC) gene in response to the
cold stress of M. rosenbergii, we investigated the
effects of RNA interference (RNAi) with the ACC gene on
the expression of fatty acid metabolism-related genes
and the mortality of M. rosenbergii under cold stress.
The results showed that different siRNA sequences and
different injection concentrations had different
inhibiting effects on ACC gene expression, and
siRNA-III with an injection concentration of 2.0
{\mu}g/g (siRNA/prawn body weight) had the best
interference effect. With the optimal siRNA and the
optimal concentration under cold stress, the
expressions of three fatty acid metabolism-related
genes, FabD, echA, and ACOT, were generally
significantly down-regulated. Compared to negative
(scrambled-siRNA) and blank (PBS) control groups, the
expression of FabD in the interference group was
extremely significantly down-regulated at 12 h in the
hepatopancreas and at 18 h in the muscles and gills;
EchA was highly significantly down-regulated at 6 and
12 h in the muscles and gills; and ACOT was extremely
significantly down-regulated and kept declining in the
gills. Within 6--18 h after injection under cold
stress, the mortality rate of the siRNA interference
group (75\%) was much lower than that of the negative
(95\%) or blank control group (97.5\%), and all prawns
died after 24 h. In conclusion, RNA interference with
the ACC gene inhibited the expression of some fatty
acid metabolism-related genes, and could partly improve
the tolerance of M. rosenbergii to cold stress,
indicating that the ACC gene might play an important
role in the response of M. rosenbergii to cold
stress.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Adyrbekova:2024:DCF,
author = "Kamila Adyrbekova and Silvia Perea and Ignacio
Doadrio",
title = "Development and Characterization of Fifteen
Polymorphic Microsatellite Loci for Rare and Endangered
Species within \bioname{Luciobarbus} {Heckel}, 1843
Genus in the \geoname{Aral Basin} and Their
Conservation Application",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "169",
day = "7",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050169",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/169",
abstract = "Biodiversity conservation entails not only the
preservation of specific taxa but also genetic
diversity. Despite the crucial role of molecular data
in freshwater fish conservation management, there is a
scarcity of information regarding the genetic diversity
of Luciobarbus Heckel, 1843 (Actinopterygii,
Cyprinidae) populations in the Aral system. Therefore,
the primary aim of this study was to provide genetic
information on two native species of the Luciobarbus
genus found in the Aral system: L. conocephalus
(Kessler, 1872) and L. brachycephalus (Kessler, 1872).
These species, like many others in the Aral system,
confront the imminent threat of extinction due to
system alterations. However, genetic studies on these
species at the nuclear level are challenging because
Luciobarbus is an allotetraploid genus. Consequently,
genetic investigations thus far have focused mainly on
sequencing mitochondrial genes due to their haploid
nature. This study has successfully developed fifteen
new polymorphic microsatellite loci, which can prove to
be valuable for population genetics, conservation, and
other pertinent research on these species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hu:2024:ETC,
author = "Tingting Hu and Cunrun Ye and Zhaoyang Ning and
Tianmei Liu and Weijie Mu",
title = "Effect of Toxicity of Chromium {(VI)} Stressors Alone
and Combined to High Temperature on the
Histopathological, Antioxidation, Immunity, and Energy
Metabolism in Fish \bioname{Phoxinus lagowskii}",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "168",
day = "5",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050168",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/168",
abstract = "Fish in aquatic ecosystems are often impacted by
environmental stressors like temperature fluctuations
and exposure to heavy metals. Chromium (Cr 6+) is a
known environmental pollutant that poses a threat to
aquatic life. Various environmental factors, such as
water temperature, have been found to affect the
toxicity of dissolved chemicals in aquatic ecosystems.
We investigated the toxicity of combinations of
different concentrations of hexavalent chromium (Cr 6+
) with high temperatures in fish. Hematological indices
demonstrated changes in white blood cells (WBCs),
hematocrit (HCT), red blood cells (RBCs), and
hemoglobin (Hb) levels during the exposure. The
qualitative and semi-quantitative analyses of different
tissues confirmed that higher concentrations of Cr 6+
caused more significant damage than lower
concentrations, with evident alterations observed in
circulatory and regressive aspects. Furthermore, brain
acetylcholinesterase levels decreased in both single
heavy metal exposure and combined exposure at a high
temperature. The activity of antioxidant oxidase and
immunological parameters increased in all treatment
groups compared with the control group following
long-term exposure. A significant and increased effect
of Cr 6+ in the high-temperature groups was observed on
the evaluated biomarkers, suggesting a possible
synergistic effect between Cr 6+ and increased
temperature. The integrated biomarker response (IBR)
reported the highest level of stress at 10 mg/L Cr 6+
combined with high temperature. The IBR analysis
revealed that the highest activity of response enzymes,
such as acid phosphatase (ACP), superoxide dismutase
(SOD), and glutathione S-transferases (GST), was
observed in the liver, whereas the gills displayed
alkaline phosphatase (ALP), GST, and SOD activity, and
the kidneys demonstrated SOD, ACP, and aspartate
aminotransferase (AST) to be most active. Through
histopathology, antioxidant enzymes, and metabolism-
and immunity-related enzymes, we determined that high
temperatures enhance the potential toxicity of Cr 6+ in
fish. We recommend conducting a thorough assessment of
the impact of climate change, particularly temperature
fluctuations, when studying the toxic effects of metal
pollution, like chromium, in aquatic ecosystems.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Onomu:2024:RFF,
author = "Abigail John Onomu and Grace Emily Okuthe",
title = "The Role of Functional Feed Additives in Enhancing
Aquaculture Sustainability",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "167",
day = "5",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050167",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/167",
abstract = "Aquaculture serves as a source of protein and
livelihood and is an alternative to capture fisheries,
thereby reducing pressure on the wild. However,
aquaculture tends to be limited by sustainability
issues, which include overdependency on fishmeal, the
high cost associated with fishmeal, the environmental
impact of aquaculture activities, which may be
detrimental to aquatic lives and the environment, and
the use of antibiotics to treat diseases, which may
have an adverse effect in their host or the
environment. Efforts are being made toward attaining
practical ways to enhance aquaculture sustainability.
One such effort is using functional feed additives in
feed formulation. Functional feed additives are dietary
ingredients incorporated in feed formulations, not only
for the usual provision of basic nutritional
requirements as offered by traditional feed but also
for growth and health enhancement; environmental and
economic gain. This review emphasizes the importance of
incorporating functional feed additives such as
probiotics, prebiotics, symbiotics, and phytogenics.
This study evaluates and presents holistic information
on functional additives, their roles in enhancing
aquaculture sustainability, and the challenges
encountered in their application.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sanchez-Caballero:2024:ACV,
author = "Carlos Adri{\'a}n S{\'a}nchez-Caballero and Jos{\'e}
Manuel Borges-Souza and Ricardo Javier
Saldierna-Mart{\'\i}nez and Avigdor Abelson",
title = "Assessing the Conservation Value of Artificial and
Natural Reefs via Ichthyoplankton Spatio-Temporal
Dynamics",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "166",
day = "4",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050166",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/166",
abstract = "The distribution of fish eggs and larvae
(ichthyoplankton) reflects spawning and nursery areas
as well as dispersal routes. This study's goal is to
demonstrate how the identification of ichthyoplankton
species and stages and their spatial distribution among
natural reefs (NRs) and artificial reefs (ARs) may
serve as decision-making tools in conservation and
fishery management. Natural reefs exhibited an
eight-times higher abundance of eggs, as well as the
highest abundance of larvae in the yolk-sac and
preflexion phases. In contrast, ARs had the highest
abundance of larvae in the flexion and postflexion
phases. Natural reefs may serve as breeding grounds for
Scaridae, Labridae, and Mugilidae; whereas, ARs may
serve as breeding sites for Lutjanidae, Synodontidae,
Carangidae, Fistularidae, and Haemulidae. Our study
revealed differences between ARs and NRs, which
demonstrate the potential of artificial reefs to expand
the supply and settlement options of reef fishes and
consequently can lead to increased fish production with
potential benefits to adjacent fishing areas through
connectivity. Thus, ARs as no-take sites can be
effective tools for both fishery management and
biodiversity conservation. The findings highlight the
potential use of ichthyoplankton tools and the
importance of considering both types of reefs in marine
conservation and management efforts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Checa:2024:CAA,
author = "Daniel Checa and Brett M. Macey and John J. Bolton and
Marissa Brink-Hull and Pauline O'Donohoe and Alessandro
Cardozo and Luis Henrique Poersch and Inmaculada
S{\'a}nchez",
title = "Circularity Assessment in Aquaculture: The Case of
Integrated Multi-Trophic Aquaculture {(IMTA)} Systems",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "165",
day = "4",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050165",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/165",
abstract = "Aquaculture is a strategic sector that aims to meet
the increased demands for healthy food for current and
future populations. However, this progression needs to
be sustainable, which can potentially be achieved by
the implementation of circular practices. Integrated
multi-trophic aquaculture (IMTA) systems promote the
incorporation of circular principles. Nevertheless, the
lack of harmonized definitions and standards impedes
the quantification of these circular attributes. This
study aims to explore the potential principles embedded
in IMTA and the existing alternatives to quantify
circularity. Two basic pillars (nutrient management and
resource use efficiency) were identified as the most
relevant circularity attributes for IMTA systems and
were quantified through aquaculture-specific
indicators. Bioremediation indicators, together with
the efficiency indicators in terms of feed, water,
energy, and infrastructure materials used, were
selected to evaluate the circularity performance of
four IMTA trials in three aquaculture facilities in
Ireland, Brazil, and South Africa. Salmon, white
shrimp, tilapia, abalone, and sea urchins were studied
and cultivated together in various combinations with
several low-trophic species in these IMTA trials to
evaluate the improvement in circularity compared with
corresponding monoculture conditions. The results
showed an increase in circularity of up to 90\% in
terms of water recirculation, as well as
bioremediation, which was improved by 80\%--90\%,
providing evidence for the potential role of IMTA in
the circularity transition.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Martinez-Bautista:2024:GBM,
author = "Gil Martinez-Bautista and Pamela Padilla and Warren W.
Burggren",
title = "Genetic Basis for Morphological Variation in the
Zebrafish \geoname{Danio rerio}: Insights from a
Low-Heterozygosity Line",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "164",
day = "2",
month = may,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050164",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/164",
abstract = "Data variability complicates reproducibility and the
interpretation of experimental results. Different
animal models have been employed to decrease
variability to enhance experimental power. However,
variation frequently persists among and within
strains/lines. In zebrafish (Danio rerio), inbred lines
(e.g., NHGRI-1) derived from wild-type lines have been
produced to greatly decrease genetic variation, with
the goal of providing better understanding of genetic
backgrounds that may influence the experimental outcome
of studies employing such lines. We hypothesized that
variations in morphological phenotypes shaped by
environmental stressors early in development are
correlated with the intrinsic degree of genetic
variability of zebrafish lines. We compared
morphological variability (yolk--chorion ratio, body
mass, embryo mass, total length, condition factor, and
specific growth rate) in wild-type AB and NHGRI-1
zebrafish lines as a function of their responses to
altered temperature and oxygen availability during the
first 7 days post-fertilization. Overall, both lines
showed similar developmental trajectories for
yolk--chorion ratio, embryo mass, and total length.
Additionally, condition factor and specific growth rate
showed similar responses within each line, regardless
of temperature and hypoxia. Importantly, the
coefficient of variation for each variable was
significantly lower in NHGRI-1 than AB larvae for 151
of 187 assessed morphological endpoints. Thus, the
low-heterozygosity NHGRI-1 zebrafish line can be useful
for decreasing inter-individual variation in
morphological responses to environmental stressors,
thereby aiding in the interpretation of results and
enhancing experimental reproducibility.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Vaibarova:2024:SVF,
author = "V{\v{e}}ra Vaibarov{\'a} and Alois
{\v{C}}{\'\i}{\v{z}}ek",
title = "Supposed Virulence Factors of Flavobacterium
psychrophilum: a Review",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "163",
day = "30",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050163",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/163",
abstract = "Flavobacterium psychrophilum is currently one of the
most important pathogens in aquaculture worldwide,
causing high losses to farmed salmonids particularly
during early growth stages with significant economic
impact. Despite previous attempts, no effective vaccine
has been developed, and protection against introduction
into farms is difficult due to the ubiquitous
occurrence of the pathogen. A better understanding of
the mechanism of disease development is essential for
targeted therapeutic and preventive measures in farms.
Unfortunately, the pathogenesis of diseases caused by
F. psychrophilum has not been elucidated yet.
Previously, several putative virulence factors have
been identified. Some appear to be essential for
disease development, while others are probably
dispensable. The importance of some factors has not yet
been explored. This review focuses on the supposed
virulence factors of F. psychrophilum and the current
knowledge about their importance in the pathogenesis of
the disease.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hernandez:2024:QTB,
author = "Jes{\'u}s M. Rivera Hern{\'a}ndez and Virginia R.
Shervette",
title = "Queen Triggerfish \bioname{Balistes vetula} Age-Based
Population Demographics and Reproductive Biology for
Waters of the \geoname{North Caribbean}",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "162",
day = "29",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050162",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/162",
abstract = "Queen triggerfish Balistes vetula is an important
reef-associated species for commercial fisheries in the
U.S. Caribbean. It exhibits a relatively unique
reproductive strategy as a nesting benthic spawner,
investing substantial energy in territorial defense,
building and maintaining nests, and caring for
fertilized eggs during the reproductive season. Prior
to this study, no comprehensive life history
information existed in the literature for queen
triggerfish. This study provides the first
comprehensive documentation of age, growth, size/age at
sexual maturity, reproductive seasonality, and
reproductive output for a Balistes species in the
Caribbean. From 2013 to 2023, we collected 2190 fish
samples from fisheries-dependent and -independent
sources from the waters of Puerto Rico and the U.S.
Virgin Islands. Fish ranged from 67 to 477 mm fork
length (FL). We documented that queen triggerfish is
sexually dimorphic with males attaining larger mean
sizes-at-age compared to females and the species is
characterized by a moderately young age at median
sexual maturity (A 50 = 3.3 y). The maximum age for our
U.S. Caribbean samples was 23 y based on increment
counts from otoliths. Spawning season encompassed the
months of December to August in the region, and female
spawning frequency ranged from an estimated 2 to 84
times per year; female spawning frequency increased
with increasing size and age of fish. We documented
that commercial fishers in the U.S. Caribbean mainly
target ``plate-size'' individuals, defined in our study
as 235--405 mm FL, which appears to act as a
self-imposed slot size range limit and results in the
fishery not removing individuals in the smallest and
largest size groups at high rates. The percentage of
immature fish from fisheries-dependent sources was
close to 0 (0.8\%). Commercial fishing for queen
triggerfish in the region currently appears to be
sustainable, but monitoring of the population should
continue.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jiang:2024:DAE,
author = "Senping Jiang and Xinyi Chen and Pengfei Li and Haoxue
Wang and Jiji Li and Kaida Xu and Yingying Ye",
title = "Does the Artificial Enhancement and Release Activity
Affect the Genetic Diversity of Marbled Rockfish
\bioname{Sebastiscus marmoratus} in \geoname{Zhoushan}
Waters?",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "161",
day = "28",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050161",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/161",
abstract = "Artificial enhancement and release activity is an
important method in the restoration of fishery
resources. In order to understand the possible genetic
effect of hatchery-released populations on wild
populations during the artificial enhancement and
release activities of Sebastiscus marmoratus in
Zhoushan waters, we utilized mitochondrial DNA control
region sequences to examine the genetic diversity in
four S. marmoratus populations, including one farmed
population, one released population and two wild
populations. A total of 68 haplotypes from 123
individuals were detected, including 3 shared
haplotypes. Haplotype diversity ranged from 0.944 to
0.980, with a mean of 0.966. The nucleotide diversity
ranged from 0.020 to 0.025, with a mean of 0.022.
Analysis of Molecular Variance (AMOVA) indicated that
the primary genetic variation occurs within populations
and the index of genetic differentiation between
populations (F ST) among the four populations showed no
differentiation. The results indicate that the current
artificial enhancement and release has not impacted the
S. marmoratus population in Zhoushan waters. Continued
long-term monitoring is essential to protect the
high-quality germplasm resources of S. marmoratus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hala:2024:ASF,
author = "Edmond Hala and Rigers Bakiu",
title = "\geoname{Adriatic Sea} Fishery Product Safety and
Prospectives in Relation to Climate Change",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "160",
day = "28",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050160",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/160",
abstract = "This bibliographic study addresses key aspects related
to fishing, product safety, and climate change in the
Adriatic Sea region. The examination of product safety
focuses on the assessment of contaminants originating
from human activities such as industry, mining,
agriculture, and household waste disposal. The
contamination of the aquatic environment has emerged as
a pressing global concern, extending to the Adriatic
basin. Aquatic organisms, including fish, are prone to
accumulating pollutants directly from polluted water
sources and indirectly through the food web. The
bio-accumulation of potentially hazardous substances,
particularly heavy metals, pesticides, PCBs, PAHs, and
antibiotic resistance in aquatic organisms, poses a
significant threat to human health. Climate change
effects will deplete our seafood supply in terms of
quantity and safety owing to negative consequences such
as higher levels of pollution, parasites, viruses,
infections, acidification, and toxicities such as
shellfish poisoning. Global food safety strategies
should be developed to reduce greenhouse gas emissions
and promote environmentally friendly technology, which
indirectly affects seafood quality and microbiological
safety, especially for the Adriatic Sea, which is part
of the Mediterranean Sea, characterized by the most
polluted waters in the world.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2024:MIN,
author = "Die Li and Xiaojuan Cui and Shuailin Chen and Jia Xu
and Yujing Li and Qiongyu Zhang and Yuandong Sun",
title = "Mechanistic Insights into Nonylphenol Stress on
\gene{BMP2} and \gene{BMP4} Gene Expression in Red
Crucian Carp (\bioname{Carassius auratus} Red var.)",
journal = j-FISHES,
volume = "9",
number = "5",
pages = "159",
day = "28",
month = apr,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9050159",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:25 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/5/159",
abstract = "Nonylphenol (NP) is a known endocrine-disrupting
chemical (EDC) that has been shown to affect bone
development in mammals. However, the detrimental
impacts of NP on the skeletal growth and development of
aquatic species, especially bony fish, remain poorly
understood. Bone morphogenic proteins (BMPs), essential
for bone formation and osteoblast differentiation, act
through the BMP-Smad signaling pathway. In this study,
two BMP genes, BMP2 and BMP4, were cloned and
characterized in the red crucian carp (Carassius
auratus red var.). The full-length cDNAs of BMP2 and
BMP4 were 2029 bp and 2095 bp, respectively, encoding
polypeptides of 411 and 433 amino acids, and share a
typical TGF-{\beta} domain with other BMPs. The tissue
expression patterns of both genes were identified,
showing ubiquitous expression across all studied
tissues. Additionally, the exposure of embryos or adult
fish to NP stress resulted in a downregulation of BMP2,
BMP4, and other genes associated with the BMP-Smad
signaling pathway. Moreover, the combined treatment of
adult fish with NP and the specific BMP receptor
inhibitor significantly reduced these genes'
expression. These findings elucidate the mechanism of
NP stress on BMP2 and BMP4, suggesting a role for the
BMP-Smad signaling pathway in the response to
endocrine-disrupting chemicals in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kovacic:2024:FMC,
author = "Marcelo Kova{\v{c}}i{\'c} and Vasilis Gerovasileiou
and Robert A. Patzner",
title = "Fishes in Marine Caves",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "243",
day = "20",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060243",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/243",
abstract = "Fishes in marine caves have attracted limited
attention by the scientific community in comparison to
subterranean fishes which have lost eyes and
pigmentation. They constitute a largely unexplored
component of marine fish diversity, except for the
relatively well-studied marine caves of the
Mediterranean Sea. These habitats are characterized by
steep environmental gradients of decreasing light and
decreasing water exchange. The fishes recorded so far
in marine caves are not exclusive residents of this
habitat and they are also present at least in the other
mesolithial habitats. In the Mediterranean marine
caves, 132 fishes have been recorded to date,
representing about 17\% of the total Mediterranean fish
species richness. Most of these species are reported
from the anterior cave zones where some light still
exists, while a smaller number of species are known
from the totally dark zones. Among them, 27.3\% are
accidental visitors, 53.8\% are the regular mesolithial
visitors and switchers between mesolithion and open
water, 5.3\% are permanent residents of the
mesolithion, but also occur in other habitats, and
13.6\% are exclusive permanent residents of
mesolithion. Some mesolithial exclusive permanent
residents recorded in marine caves share similar
morphology, probably as adaptations to these
habitats.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2024:DND,
author = "Yinjia Li and Zeyuan Hu and Yixi Zhang and Jihang Liu
and Wan Tu and Hong Yu",
title = "{DDEYOLOv9}: Network for Detecting and Counting
Abnormal Fish Behaviors in Complex Water Environments",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "242",
day = "20",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060242",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/242",
abstract = "Accurately detecting and counting abnormal fish
behaviors in aquaculture is essential. Timely detection
allows farmers to take swift action to protect fish
health and prevent economic losses. This paper proposes
an enhanced high-precision detection algorithm based on
YOLOv9, named DDEYOLOv9, to facilitate the detection
and counting of abnormal fish behavior in industrial
aquaculture environments. To address the lack of
publicly available datasets on abnormal behavior in
fish, we created the ``Abnormal Behavior Dataset of
Takifugu rubripes '', which includes five categories of
fish behaviors. The detection algorithm was further
enhanced in several key aspects. Firstly, the DRNELAN4
feature extraction module was introduced to replace the
original RepNCSPELAN4 module. This change improves the
model's detection accuracy for high-density and
occluded fish in complex water environments while
reducing the computational cost. Secondly, the proposed
DCNv4-Dyhead detection head enhances the model's
multi-scale feature learning capability, effectively
recognizes various abnormal fish behaviors, and
improves the computational speed. Lastly, to address
the issue of sample imbalance in the abnormal fish
behavior dataset, we propose EMA-SlideLoss, which
enhances the model's focus on hard samples, thereby
improving the model's robustness. The experimental
results demonstrate that the DDEYOLOv9 model achieves
high Precision , Recall , and mean~Average~Precision (
mAP) on the ``Abnormal Behavior Dataset of Takifugu
rubripes '', with values of 91.7\%, 90.4\%, and 94.1\%,
respectively. Compared to the YOLOv9 model, these
metrics are improved by 5.4\%, 5.5\%, and 5.4\%,
respectively. The model also achieves a running speed
of 119 frames per second (FPS), which is 45 FPS faster
than YOLOv9. Experimental results show that the
DDEYOLOv9 algorithm can accurately and efficiently
identify and quantify abnormal fish behaviors in
specific complex environments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Alesci:2024:EID,
author = "Alessio Alesci and Davide {Di Paola} and Sebastian
Marino and Federica {De Gaetano} and Marco Albano and
Silvana Morgante and Gabriele Rigano and Letterio
Giuffr{\`e} and Magdalena Kotanska and Nunziacarla
Span{\`o} and Eugenia Rita Lauriano",
title = "Exploring the Internal Defense System of
\bioname{Cerastoderma glaucum} ({Brugui{\`e}re}, 1789)
Exposed to Pristine Microplastics: The Sentinel Role of
Haemocytes as Biomarkers",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "241",
day = "20",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060241",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/241",
abstract = "The ubiquitous presence of microplastics (MPs) in the
environment is a major concern for the conservation of
biodiversity. Numerous studies show the detrimental
effects of MPs on marine species, especially because of
their small size and their capacity to absorb organic
contaminants from their surroundings. The bivalve
mollusk Cerastoderma glaucum (Brugui{\`e}re, 1789),
because of its wide geographic distribution and
immobile feeding habits, can be used as a sentinel and
bioindicator species. By examining the presence,
localization, and co-localization of Toll-like receptor
2 (TLR2) and inducible nitric oxide synthetase (iNOS),
this study aims to evaluate the response of the
internal defense system of C. glaucum to pristine MPs
through the employment of confocal microscopy and
bioinformatics techniques. The results show haemocytes
immunoreactive to the antibodies tested; in particular,
a higher number of TLR2-positive haemocytes can be
observed in the group exposed to pristine MPs. These
findings suggest that haemocytes can play a key
biomarker role as sentinels to environmental
pollutants. Furthermore, bioinformatics analyses on the
antibodies tested confirmed an evolutionary
conservation of these molecules. These data highlight
the critical role of phagocytosis in identifying
ecosystem damage and are helpful in developing
biosensors with less negative effects on the
environments in which they are applied.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Mori:2024:GMH,
author = "Natacha C. Mori and Bruna T. Michelotti and Caio C. F.
Magnotti and Caroline A. Bressan and Let{\'\i}cia B.
Bianchin and Fernando J. Sutili and Ana Paula G.
Almeida and Luiz C. Kreutz and Maria A. Pavanato and
Vinicius R. Cerqueira and Silvio T. da Costa and
Bernardo Baldisserotto and Berta M. Heinzmann",
title = "Growth, Metabolic, Hepatic and Redox Parameters,
Digestive Enzymes and Innate Immunity in \bioname{Mugil
liza} Fed a Citral-Supplemented Diet",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "240",
day = "20",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060240",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/240",
abstract = "Mugil liza juveniles (6.69 {\pm} 0.06 g) were
subjected to dietary citral (0-control, 0.5, 1.0 and
2.0 mL per kg feed) for 45 days, and its possible
effects on zootechnical and metabolic parameters,
digestive enzymes, innate immunity, oxidative status
and liver damage were evaluated. At the end of the
experiment, fish fed 2.0 mL citral per kg feed showed a
greater weight gain and protein retention efficiency,
as well as enhanced activities of pepsin (stomach) and
amylase (intestine) compared with control fish. Citral
supplementation decreased liver lipoperoxidation and
increased the activities of glutathione peroxidase,
glutathione-S-transferase and superoxide dismutase in
the gills, liver and brain. The highest level of citral
inclusion augmented non-protein thiol content in the
brain and gills. Myeloperoxidase activity was lower in
fish offered 1.0 and 2.0 mL citral per kg feed. Dietary
citral did not influence the plasma levels of aspartate
aminotransferase and alanine aminotransferase or the
hepatic histology. As it improved growth, the activity
of digestive enzymes and general health, dietary citral
may be recommended for M. liza at 2.0 mL per kg feed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:TAJ,
author = "Changlin Liu and Zheng Zhang and Shouyong Wei and
Wenjie Xiao and Chao Zhao and Yue Wang and Liguo Yang",
title = "Transcriptome Analysis of Juvenile Black Rockfish
\bioname{Sebastes schlegelii} under Air Exposure
Stress",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "239",
day = "19",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060239",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/239",
abstract = "The study aimed to uncover the molecular response of
juvenile Sebastes schlegelii to air exposure stress by
identifying differentially expressed genes (DEGs) that
may underlie their anti-stress mechanisms. Juvenile
Sebastes schlegelii were subjected to varying durations
of air exposure stress. The total RNA was extracted
from whole tissues and sequenced using the Illumina
NovaSeq 6000 platform. The transcriptome data were
analyzed to identify DEGs through pairwise comparisons
across a control group and two experimental groups
exposed to air for 40 s and 2 min 30 s, respectively.
The comparative DEG analysis revealed a significant
number of transcripts responding to air exposure
stress. Specifically, 5173 DEGs were identified in the
40 s exposure group (BS) compared to the control (BC),
6742 DEGs in the 2 min 30 s exposure group (BD)
compared to the control (BC), and 2653 DEGs when
comparing the BD to the BS group. Notably, Gene
Ontology (GO) analysis showed an enrichment of DEGs
associated with peptidase activity and extracellular
regions, suggesting a role in the organism's stress
response. Kyoto Encyclopedia of Genes and Genomes
(KEGG) pathway analysis pointed to the involvement of
metabolic pathways, which are crucial for energy
management under stress. The upregulation of protein
digestion and absorption pathways may indicate a
physiological adaptation to nutrient scarcity during
stress. Additionally, the identification of antibiotic
biosynthesis pathways implies a potential role in
combating stress-induced infections or damage. The
identified DEGs and enriched pathways provide insights
into the complex anti-stress response mechanisms in
juvenile Sebastes schlegelii. The enrichment of
peptidase activity and extracellular region-related
genes may reflect the initiation of tissue repair and
immune response following air exposure. The connection
between protein digestion and absorption pathways and
anti-stress capabilities could be interpreted as a
metabolic readjustment to prioritize energy-efficient
processes and nutrient assimilation during stress. The
role of antibiotic biosynthesis pathways suggests a
defensive mechanism against oxidative stress or
microbial invasion that might occur with air
exposure.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Poisson:2024:PIH,
author = "Fran{\c{c}}ois Poisson and Jim R. Ellis and Sophy R.
McCully Phillips",
title = "Preliminary Insights on the Habitat Use and Vertical
Movements of the Pelagic Stingray
(\bioname{Pteroplatytrygon violacea}) in the
\geoname{Western Mediterranean Sea}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "238",
day = "18",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060238",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/238",
abstract = "Pelagic stingray (Pteroplatytrygon violacea) is the
only species of stingray (Dasyatidae) that utilizes
both pelagic and demersal habitats. It is the main
bycatch species in pelagic longline fisheries targeting
bluefin tuna (Thunnus thynnus) in the Gulf of Lions. In
the Mediterranean Sea, their stock structure,
behavioural ecology and movements are unknown. For the
first time in the Mediterranean, 17 individuals (39--60
cm disc width) were tagged with pop-up satellite
archival transmitting tags using a novel method of tag
attachment to investigate horizontal and vertical
movements. The tags were attached for between two and
60 days. Between the months of July and October,
pelagic stingray occupied a temperature range of
12.5--26.6 {$^\circ $}C, and a depth range extending
from the surface to 480 m. Monthly trends in
catch-per-unit-effort (CPUE) of pelagic stingray peaked
in August and decreased by late autumn. Pelagic
stingray may aggregate on the continental shelf during
summer and move southwards in early autumn, and this
movement pattern is considered in relation to the
reproductive cycle and overwintering. At-vessel
mortality was low, but there was varying evidence of
post-release mortality, indicating the need for further
work. Future work and options for bycatch mitigation
are also discussed.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Tsaousi:2024:FRR,
author = "Nefeli Tsaousi and Stefanos Kalogirou",
title = "First Record of the Red Cornetfish \bioname{Fistularia
petimba} {Lacep{\`e}de,} 1803 from \geoname{Amorgos
Island (Central Aegean Sea; Greece)} and a Review of
Its Current Distribution in the \geoname{Mediterranean
Sea}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "237",
day = "18",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060237",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/237",
abstract = "The rapid spread of non-native species (NNS) poses a
significant threat to biodiversity globally, with the
Mediterranean region being particularly susceptible due
to increased human activities and its status as a
marine biodiversity hotspot. In this study, we focus on
the introduction and distribution of Fistularia
petimba, a member of the Fistulariidae family, in the
eastern Mediterranean Sea and a record from the coasts
of Amorgos Island, Greece. Through a baseline fishery
study conducted over 12 months, utilizing experimental
sampling with gillnets, trammel nets, and longlines,
one individual of F. petimba was captured off the coast
of Katapola Bay. Morphological examination confirmed
its identity, with measurements on meristic
characteristics obtained and the stomach content
analysed. This finding represents a significant
addition to the documented distribution of F. petimba
in the Mediterranean Sea, particularly in the Aegean
Sea, underscoring the importance of ongoing research in
uncovering new occurrences and expanding our
understanding of marine biodiversity and ecosystem
changes. Further investigation into the ecological
preferences and population dynamics of F. petimba in
the Aegean Sea is crucial for informed conservation and
management efforts if this species is considered to be
established.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:TAR,
author = "Jun Liu and Songyue You and Yuting Wang and Jie Bao
and Hongbo Jiang",
title = "Transcriptional Analysis Reveals the Iron Regulation
Network of the Pathogenic Yeast \bioname{Metschnikowia
bicuspidata} in Response to Iron Stress",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "236",
day = "18",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060236",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/236",
abstract = "Metschnikowia bicuspidata, a globally distributed
opportunistic pathogenic fungus, poses a significant
threat to crustaceans in diverse aquatic ecosystems,
causing severe diseases. Iron, recognized as a
virulence factor, plays a crucial role in successful
infection with M. bicuspidata. Therefore, this study
aims to investigate the transcriptome response of M.
bicuspidata to low- and high-iron conditions. Overall,
1082 differentially expressed genes (DEGs) (FDR {$<$}
0.05, |log 2 FC {\geq} 1.5|) were identified,
comprising 977 and 105 DEGs, in response to low- and
high-iron conditions, respectively. These genes
predominantly participate in altering metabolism, cell
membranes, or cellular structure, allowing the organism
to adapt to varying iron levels. Iron
limitation-induced genes play crucial roles in energy
metabolism, transport, and catabolism pathways.
Moreover, 27 ortholog genes were associated with iron
transport and homeostasis, with 7 of them participating
in iron uptake and regulation under low-iron
conditions. This study contributes to the comprehension
of iron homeostasis in aquatic fungi. It may offer
potential therapeutic strategies for managing M.
bicuspidata diseases.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Honji:2024:AVN,
author = "Renato M. Honji and Bruno C. Ara{\'u}jo and Paulo H.
de Mello and Mart{\'\i}n R. Ramallo and Leonel
Morandini and Danilo Caneppele and Renata G. Moreira",
title = "Arginine-Vasotocin Neuronal System in
\bioname{Steindachneridion parahybae} ({Siluriformes:
Pimelodidae}) and Its Influence on Artificially Induced
Spawning in Captivity",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "235",
day = "18",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060235",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/235",
abstract = "This study summarizes new data on induced spawning of
Steindachneridion parahybae, focusing on the aggressive
behavior of females. This study characterizes the
vasotocinergic system using immunohistochemistry,
highlighting the potential influence of
arginine-vasotocin (AVT) on reproductive physiology.
Two experimental groups were proposed: (A) control,
with one female in the aquarium, and (B) experimental,
with two females in the same aquarium. Dominant (D)
females presented a more aggressive behavior and did
not show any injury. They apparently had a length and
body mass higher than injured nondominant (ND) females.
The analysis identified positive AVT immunoreactive
(ir) neurons exclusively within the preoptic area,
including parvocellular, magnocellular, and
gigantocellular subpopulations, containing fibers-ir
extending into the pituitary gland. Cellular and
nuclear areas were greater in D compared to ND in the
magnocellular subpopulation. There were no differences
between parvocellular and gigantocellular
subpopulations. There was a difference on the steroid
plasma profile of cortisol (more in ND than in D) and
17{\alpha},20{\beta}-dihydroxy-4-pregnen-3-one (more in
D than in ND). Furthermore, control and D females
presented higher optical densities for AVT-ir,
gonadotropin-releasing hormone-ir, and luteinizing
hormone-ir than ND. In general, there were no
differences in the results of female (control group)
with D females. The AVT system is highly complex,
possibly counting multiple sites of action during
artificial reproduction and acting directly and/or
indirectly associated with behavioral and physiological
changes in S. parahybae females when induced to
spawning.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Emadi:2024:SPA,
author = "Cameron M. Emadi and Preston T. Bean and Edward M.
Mager",
title = "Swimming Performance Assessments of Fish Species of
Greatest Conservation Need to Inform Future Stream
Crossing Designs in \geoname{Texas}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "234",
day = "18",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060234",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/234",
abstract = "Conventional structures associated with stream
crossings such as bridges and culverts can lead to
zones of high-velocity water flow that impede fish
passage. Such obstacles are likely to harm native fish
populations by impacting migrations critical to their
life history strategies, causing habitat fragmentation
and extirpation, thereby limiting population growth and
distribution. Due to the high rates of human population
growth and development occurring in Texas, this is an
issue of particular concern for fish designated as
species of greatest conservation need (SGCN). This
project focused on four SGCN fishes native to the
Edwards Plateau Ecoregion, namely the Guadalupe Bass,
Guadalupe Roundnose Minnow, Guadalupe Darter, and
Plateau Shiner, at both adult (all species) and
juvenile (Guadalupe Bass only) life stages. Our primary
aim was to aid in the design of future culverts
associated with stream crossings (or the modification
of existing culverts) so that the water velocities
through these structures do not exceed the swimming
capacities of our target SGCN fishes. To this end, we
assessed their maximum sustained swimming speeds (U
crit) under a typical range of Edwards Plateau stream
temperatures (15, 22.5, and 30 {$^\circ $}C) to be used
in site-specific calculations of the maximum allowable
culvert water velocities (Vf). A secondary objective
was to collect physiological endpoints of relevance to
the overall swimming performance including the maximum
burst swimming speeds (U max), metabolic rate
measurements (i.e., standard metabolic rate (SMR),
maximum metabolic rate (MMR), and aerobic scope (AS)),
cost of transport (COT), and optimal swimming speed (U
opt). Temperature-related effects were observed that
can be used to inform site-specific culvert designs. In
general, the highest U crit values for the tested
species were near, or possibly between, 22.5 and 30
{$^\circ $}C, while U max stayed relatively consistent
among treatments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jackson:2024:MLC,
author = "George D. Jackson and Christine H. Jackson and Maura
Brandao and Adam K. Jackson and Eduardo Espinoza and
Monica Soria-Carvajal",
title = "Mercury Levels in the Crab \bioname{Grapsus grapsus}
across the \geoname{Gal{\'a}pagos Archipelago}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "233",
day = "18",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060233",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/233",
abstract = "The levels of mercury (Hg) were examined in the leg
muscle of the crab Grapsus grapsus from five sites on
four islands within the Gal{\'a}pagos archipelago.
Mercury values obtained using a Milestone DMA 80 evo
direct mercury analyzer varied both within and among
sites. Total mercury concentrations (mg kg {$-$1} dry
weight) ranged from the lowest at a protected location
at Isabela Island (0.06 {\pm} 0.02) to the highest at
the San Crist{\'o}bal urban location (2.04 {\pm}
0.069). Crabs from South Plaza Island also had
surprisingly high levels of mercury with a mean of 1.2
{\pm} 0.6. Values from urban sites at Isabela Island
and Academy Bay, Santa Cruz, had intermediate values.
When converted to wet weight, crabs from both San
Crist{\'o}bal and South Plaza were within or above the
levels (0.3--0.5 mg kg {$-$1} wet weight set by various
government agencies) considered potentially harmful to
human health if ingested. A lesser number of both
oysters and barnacles were also analyzed for mercury at
South Plaza and Academy Bay, and while the values were
lower compared to the crabs, they followed the same
pattern of elevated levels at South Plaza compared to
Academy Bay. It is unclear as to whether the mercury
comes from natural sources, such as volcanism, or
anthropogenic causes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huynh:2024:PWT,
author = "Thi Kim Duyen Huynh and Marie-Louise Scippo and
Mathias Devreese and Siska Croubels and Quoc Thinh
Nguyen and Caroline Douny and Thi Hoang Oanh Dang and
Quoc Viet Le and Minh Phu Tran",
title = "Pharmacokinetics and Withdrawal Times of Cefotaxime in
White Leg Shrimp (\bioname{Litopenaeus vannamei}) after
Oral Administration",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "232",
day = "17",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060232",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/232",
abstract = "A high-performance liquid chromatography method
coupled to tandem mass spectrometry was validated in
order to study the pharmacokinetics of cefotaxime in
shrimp hepatopancreases and plasma, as well as its
withdrawal time related to a maximum residue limit
(MRL) in shrimp muscle. Pharmacokinetics parameters
were investigated through oral medication at a single
dose of 25 mg/kg shrimp body weight and subsequent
hepatopancreas and plasma cefotaxime concentration
measurements at 0.5, 1, 2, 4, 8, 12 and 24 h after
shrimp were fed with medication. The maximum
concentration of cefotaxime was observed after one hour
in the hepatopancreas (C max, 19.45 {\pm} 2.10 mg/kg)
and 4 h in plasma (0.184 {\pm} 0.061 mg/L). Based on a
minimum inhibitory concentration (MIC) of cefotaxime of
4.13 mg/L against Vibrio parahaemolyticus (known to
cause acute hepatopancreatic necrosis disease (AHPND)
in white leg shrimp), it was observed that the time
during which the hepatopancreas cefotaxime
concentration was above the MIC was 23 h. An every 24 h
cefotaxime treatment could thus be effective in
fighting against this bacterium in shrimp. The
withdrawal time of cefotaxime was determined after
shrimp were fed with medicated feed once a day and
twice a day for three consecutive days. Shrimp muscle
was collected on day 1 and day 3 during medication and
1, 3, 7, 14 and 21 days after medication was stopped.
Considering an MRL of 50 {\mu}g/kg, the withdrawal
times were 8.5 degree-days (corresponding to 6.9 h at
29.5 {$^\circ $}C) after shrimp were fed with medicated
feed once a day for 3 days and 95.5 degree-days (77.7 h
at 29.5 {$^\circ $}C) after shrimp were fed with
medicated feed twice a day for 3 days. Moreover,
histological analysis revealed that feeding shrimp with
cefotaxime at the given dose in once- or twice-a-day
treatments did not negatively impact the shrimp
hepatopancreas.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Hicks:2024:EED,
author = "Grace L. Hicks and Phillip S. Lobel",
title = "An Examination of the Effects of Dorsal Fin-Mounted
{SPOT} on \bioname{Carcharodon carcharias}, the Great
White Shark",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "231",
day = "17",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060231",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/231",
abstract = "This opinion piece presents empirical evidence to
examine possible negative consequences of the use of
penetrative tagging as used on the great white shark
(GWS). Tagging programs currently using this method
attach SPOT (Spatial Positioning Only Tags) using
corrodible bolts inserted through the dorsal fin while
the shark is taken out of water. Such methods can cause
harm to the tagged individual. Possible adverse effects
include impacts on growth, tag biofouling, wounds,
heightened stress, and hemorrhaging. This method may
adversely impact dorsal fin structure and the shark's
hydrodynamics. As a result, data collected may not be
reflective of natural behavior. Bolted SPOT are
semi-permanently affixed to the shark but can have a
battery life of approximately 3.5 years. Most of these
tags (69\%) ceased transmitting in less than 2 years.
Alternative tagging technologies exist as more humane
options.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Sun:2024:TRS,
author = "Qingsong Sun and Yuran Pang and Yuhan Qin and Ziting
Dong and Yanling Ma and Yuan Zhao and Zhanning Zhang
and Jinmei Liu and Binghui Mao and Baishuang Yin",
title = "Transcriptomic Revealed That Selenium-Rich
\bioname{Lactobacillus plantarum} Alleviated
Cadmium-Induced Immune Responses in {Bulatmai} barbel
\bioname{Luciobarbus capito} Kidneys",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "230",
day = "16",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060230",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/230",
abstract = "Cadmium (Cd) is a common environmental pollutant that
accumulates mainly in the kidneys and thus endangers
the physiological health of aquatic animals. Selenium
(Se) is a natural antidote to heavy metals that
antagonises heavy metal toxicity and enhances the
antioxidant capacity of organisms. Lactobacillus
plantarum (L. plantarum) can reduce the toxicity of
heavy metals through adsorption, reduction and
metabolism. Studies have confirmed that the biological
synthesis of Se nanoparticles (Bio-SeNPs) using
bacterial microorganisms is simple, safe and less toxic
than the synthesis of inorganic and organic Se, but the
effect on Cd-induced immunosuppression is un-known. One
hundred and eighty Bulatmai barbel (Luciobarbus capito:
L. capito) plants were randomly divided into control
(C), Cd and Cd + Se-enriched L. plantarum groups
(S1L1-Cd) and fed for 28 days. The analysis methods
included histopathology, test kits, transcriptomics and
real-time quantitative PCR. The addition of
selenium-enriched L. plantarum significantly attenuated
cadmium-induced pathological changes such as glomerular
atrophy, detachment of renal tubular epithelial cells,
mild swelling, and interstitial inflammatory cell
infiltration. Cd stress can lead to significant
decreases in RBC, HCT, WBC, LZM, C3, and IgM levels,
and the addition of Se-enriched L. plantarum can
significantly reverse the changes in these indicators.
Transcriptomic analysis revealed 488 DEGs in the Cd
groups, 301 of which were upregulated and 187 of which
were downregulated. There were 1474 DEGs in the S1L1-Cd
group, of which 720 were upregulated and 754 were
downregulated. In addition, GO enrichment analysis
revealed that the biological regulation of the most
differentially expressed genes involved metal ion
binding, ATP binding and nucleotide inclusion. KEGG
enrichment analysis revealed six of the most enriched
pathways: oxidative phosphorylation, Huntington
disease, retrograde endocannabinoid signalling, natural
killer cell-mediated cyto-toxicity, the IL-17
signalling pathway, and leukocyte transient migration.
Moreover, we selected 12 DEGs for qRT-PCR, which showed
that the qRT-PCR results were consistent with our
RNA-Seq results. Our results suggest that Se-enriched
L. plantarum can enhance immunity and alleviate Cd
exposure-mediated immunosuppression in L. capito.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Wang:2024:EDS,
author = "Jing Wang and Dan Fan and Ran Zhao and Tongyan Lu and
Shaowu Li and Di Wang",
title = "Effects of Dietary Supplementation with Endogenous
Probiotics \bioname{Bacillus subtilis} on Growth
Performance, Immune Response and Intestinal
Histomorphology of Juvenile Rainbow Trout
(\bioname{Oncorhynchus mykiss})",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "229",
day = "15",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060229",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/229",
abstract = "The rainbow trout (Oncorhynchus mykiss) is an
important commercial fish. Studies have shown that
probiotics can promote the healthy growth of fish. In
this study, we assessed the impact of an endogenous
Bacillus subtilis strain (RT-BS07) on the growth,
survival and immunological response of juvenile rainbow
trout. Additionally, the morphology of rainbow trout's
intestinal tissue was measured. Control (CT) and
experimental groups (B) were fed with conventional and
probiotic-supplemented diets (1.0 $ \times $ 10 8 CFU
per gram) for 42 days, respectively. Following feeding,
a challenge experiment was carried out with Aeromonas
hydrophila at 1.0 $ \times $ 10 7 CFU/mL to determine
the experimental fish's tolerance to the bacteria. The
results showed that the weight gain rate (WGR) in group
B was 118.2\%, which was significantly higher than that
in group CT (54.86\%) after 42 days. The height of
intestinal villi and the number of goblet cells
increased in group B, thus increasing the resistance of
rainbow trout to pathogenic bacteria. The alkaline
phosphatase (AKP) activity (U/mg prot) in the liver,
spleen and intestinal tissues of group B was
significantly higher than that in the CT group. The
expression of interleukin-1{\beta} (IL-1{\beta}) in the
intestinal tissue of group B was 6-fold higher than
that in group CT. Furthermore, the expression of growth
hormone-2 (GH-2) in the spleen was 3-fold higher than
that in group CT. After 12 days of A. hydrophila
injection, the survival rate was 40\% and 70\% in group
CT and group B, respectively. Collectively, the present
study demonstrated that the addition of endogenous B.
subtilis strain (1.0 $ \times $ 10 8 CFU per gram) to
the feed can effectively promote the growth of rainbow
trout and enhance immunity, which is beneficial to
rainbow trout culture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Garcia-Meilan:2024:OSP,
author = "Irene Garc{\'\i}a-Meil{\'a}n and Ramon Fontanillas and
Joaquim Guti{\'e}rrez and Encarnaci{\'o}n Capilla and
Isabel Navarro and {\'A}ngeles Gallardo",
title = "Oxidative Status of the Pyloric Caeca and Proximal
Intestine in Gilthead Sea Bream Fed Diets Including
Different Vegetable Oil Blends from Palm, Rapeseed and
Linseed",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "228",
day = "14",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060228",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/228",
abstract = "Nowadays, including vegetable ingredients in fish
diets without growth effects is common; however, their
intestinal oxidative status under these conditions is
less known. Five isonitrogenous and isolipidic diets
with 75\% vegetable oil (VO) inclusion were formulated
for juvenile gilthead sea bream (Sparus aurata). As VO,
one diet contained palm oil (diet P), another rapeseed
oil (diet R), and the other three included linseed oil
(L) combined with the above-mentioned VOs (named PL, RL
and RPL diets). After 18 weeks, pyloric caeca (PC) and
proximal intestine (PI) were analyzed for oxidative
stress biomarkers, lipid peroxidation (LPO), and gene
expression. Dietary linseed oil diminished the
superoxide dismutase activity in both intestinal
regions, catalase in PC and glutathione reductase in
PI; rapeseed oil reduced the glutathione peroxidase
(GPx) and glutathione-S-transferase activities in PC,
and palm oil upregulated GPx activity in PI. The PL
diet triggered LPO levels in the PI, and RPL-fed fish
showed the highest levels of LPO in the PC due to lower
antioxidant activities, while RL-fed fish presented the
best oxidative status. The results suggest that the
dietary amount of n-6 and the unsaturated/saturated
fatty acids ratio are factors to be considered in
aquafeed formulation, including VOs, to improve the
intestinal oxidative status in fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Choi:2024:PEL,
author = "Hee-Jae Choi and Da-Yeon Choi and Jung-Jin Park and
Hye Jin Jung and Bo-Seong Kim and Sung-Chul Hong and
Jun-Hwan Kim and Yue Jai Kang",
title = "Protective Effects of Long Double-Stranded {RNA} with
Different {CpG} Motifs against \bioname{Miamiensis
avidus} and {Viral Hemorrhagic Septicemia Virus (VHSV)}
Infections in Olive Flounder (\bioname{Paralichthys
olivaceus})",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "227",
day = "13",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060227",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/227",
abstract = "The South Korean aquaculture industry has incurred
considerable production losses due to various
infectious diseases. Artificially synthesized
polyinosinic--polycytidylic acid (poly I:C),
structurally similar to double-stranded RNA (dsRNA) and
cytidine--phosphate--guanosine oligodeoxynucleotides,
can enhance immune responses and protect against
diseases. Here, we investigated dsRNA molecules with
different cytidine--phosphate--guanosine (CpG) motifs
(dsRNA-CpGMix) as fused agents to treat Miamiensis
avidus and viral hemorrhagic septicemia virus (VHSV)
infection in olive flounders. We further investigated
the efficacy of specific sequence motifs in dsRNA in
modulating immunostimulatory effects. Fish treated with
poly I:C or dsRNA-CpGMix exhibited higher survival
rates than the control group. Olive flounder leukocytes
stimulated with poly I:C or dsRNA-CpGMix showed
increased scuticocidal activity in the presence of
inactivated immune sera. dsRNA with CpG motif sequences
induced higher resistance against M. avidus and VHSV
infections than dsRNA without CpG motif sequences, and
the dsRNA-CpGMix group showed upregulated ISG15 or Mx
compared to the dsRNA-GFP group. Thus, dsRNA containing
CpG motifs can be used as effective immunostimulants to
enhance resistance against viral and parasitic diseases
in olive flounder. The specific sequences of the CpG
motifs in dsRNA may be important for enhancing immune
responses and resistance against M. avidus and VHSV
infections in olive flounders.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Song:2024:EVP,
author = "Jin Ah Song and Heung-Sik Park and Yun-Hwan Jung and
Dong Mun Choi and Cheol Young Choi and Dae-Won Lee",
title = "Effects of Various Photoperiods and Specific
Wavelengths on Retinal Changes and Oxidative Stress in
the Conch \geoname{Tegula rustica}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "226",
day = "13",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060226",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/226",
abstract = "To improve aquaculture practices and husbandry of a
variety of gastropods, including small conch species,
it is necessary to study the physiological and
endocrinological responses of nocturnal conches to
light exposure. In this study, we investigated the
effect of the light environment on Tegula rustica by
exposing it to contrasting light conditions and
observing histological changes in the retina and
oxidative stress according to photoperiod and light
wavelength. We confirmed that the pigment layer was
significantly thicker in the group irradiated with
light for 24 h (LL), but that its thickness did not
differ significantly with light wavelength.
Additionally, light wavelength changes did not cause a
significant change in H 2 O 2 concentration until 5
days after the change in the light environment.
However, a significantly higher H 2 O 2 concentration
was observed in the LL test group on the eighth day
compared with the other experimental groups. And a
significantly higher total antioxidant capacity and
malondialdehyde (MDA) were observed in the LL group on
the third day compared with the other experimental
groups. Our results indicate that the light environment
affects the reaction of conches and that continuous
light has a stronger effect on the thickness of the
pigment layer than the light wavelength. In addition,
continuous light irradiation induces excessive ROS and
causes oxidative stress. These results can also be
provided as basic data for husbandry when aquaculturing
gastropods.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2024:EMV,
author = "Tao Li and Ronggang Ding and Jing Zhang and Yongcan
Zhou and Chunsheng Liu and Zhenjie Cao and Yun Sun",
title = "The Establishment of the Multi-Visual Loop-Mediated
Isothermal Amplification Method for the Rapid Detection
of \bioname{Vibrio harveyi}, \bioname{Vibrio
parahaemolyticus}, and \geoname{Singapore} grouper
iridovirus",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "225",
day = "13",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060225",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/225",
abstract = "Groupers are valuable economic fish in the southern
sea area of China, but the threat of disease is
becoming more and more serious. Vibrio harveyi, V.
parahaemolyticus, and Singapore grouper iridovirus
(SGIV) are three important pathogens that cause disease
in groupers, and infection with either a single one or
a mix of these pathogens poses a serious threat to the
healthy development of grouper culture. To enhance the
rapid diagnosis and screening in the early stages, it
is necessary to develop rapid detection methods of
these pathogens. To simultaneously and rapidly detect
the three pathogens, in this study, we utilized the
TolC of V. harveyi, DNAJ of V. parahaemolyticus, and
RAD2 of SGIV as the target genes and established a
triple visual loop-mediated isothermal amplification
(LAMP) method. This LAMP method showed a detection time
as fast as 30 min and a high sensitivity of 100
fg/{\mu}L. Moreover, this method exhibited strong
specificity and no cross-reaction with seven types of
Vibrio and Staphylococcus aureus, as well as five
common viruses in aquatic animals. Then, the LAMP
products were enzymically cut, and three characteristic
strips were used to identify the pathogen species. The
results of the clinical trials demonstrated that the
method could accurately and specifically detect V.
harveyi, V. parahaemolyticus, and SGIV in grouper
tissues. In summary, this study successfully
established a triple visual LAMP rapid detection method
for V. harveyi, V. parahaemolyticus, and SGIV. The
method offers several advantages including simple
equipment, easy operation, rapid reaction, high
specificity, high sensitivity, and visual results. It
is suitable for the early and rapid diagnosis of
groupers infected with V. harveyi, V. parahaemolyticus,
and SGIV, thereby providing useful technical support
for further application in the large-scale disease
surveillance of aquaculture animals.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kadota:2024:QAA,
author = "Minoru Kadota and Shinsuke Torisawa and Tsutomu
Takagi",
title = "Quantitative Assessment and Analysis of Fish Behavior
in Closed Systems Using Information Entropy",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "224",
day = "12",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060224",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/224",
abstract = "This study introduces a method for quantitatively
assessing the complexity and predictability of fish
behavior in closed systems through the application of
information entropy, offering a novel lens through
which to understand how fish adapt to environmental
changes. Utilizing simulations rooted in a random walk
model for fish movement, we delve into entropy
fluctuations under varying environmental conditions,
including responses to feeding and external stimuli.
Our findings underscore the utility of information
entropy in capturing the intricacies of fish behavior,
particularly highlighting the synchrony in collective
actions and adaptations to environmental shifts. This
research not only broadens our comprehension of fish
behavior but also paves the way for its application in
fields like aquaculture and resource management.
Through our analysis, we discovered that smaller grid
sizes in simulations capture detailed local
fluctuations, while larger grids elucidate general
trends, pinpointing a 2.5 grid as optimal for our
study. Moreover, changes in swimming speeds and
behavioral adaptations during feeding were
quantitatively analyzed, with results illustrating
significant behavior modifications. Additionally,
employing a Gaussian mixture model helped to clarify
the nuanced changes in fish behavior in response to
altered light conditions, demonstrating the layered
complexity of fish responses to environmental stimuli.
This investigation confirms the efficacy of information
entropy as a robust metric for evaluating fish shoal
behavior, offering a fresh methodology for ecological
and environmental studies, with promising implications
for sustainable management practices.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pereira:2024:FPE,
author = "Luciana Alves Pereira and Leandro Castello and Eric
Hallerman and Edson Rubens Ferreira Rodrigues and
Carolina Rodrigues da Costa Doria and Fabrice
Duponchelle",
title = "Flood Pulse Effects on the Growth of
\bioname{Pseudoplatystoma fasciatum} in the
\geoname{Amazon Basin}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "223",
day = "12",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060223",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/223",
abstract = "Fish growth is a fundamental biological process driven
by a multitude of intrinsic (within-individual) and
extrinsic (environmental) factors that underpin
individual fitness and population dynamics. Interannual
variability in river hydrology regarding the intensity
and duration of floods and droughts can induce
interannual variations in the biotic and abiotic
variables that regulate fish growth. However, the
understanding of how interannual variability in river
hydrology affects fish growth remains limited for most
species and ecosystems. We evaluated how inter-annual
hydrological variations within the Amazon River basin
influence the growth of the catfish Pseudoplaystoma
fasciatum. Our research questions were as follows: Do
floods lead to the faster growth of P. fasciatum and
droughts lead to the slower growth? And do floods and
droughts affect all age classes in the same manner? We
sampled 364 specimens of P. fasciatum from five sites
in the Amazon basin, estimated their growth rates, and
related the growth rates to indices of the intensity of
floods and droughts. We fitted linear mixed-effects
models to test the relationship between growth
increments and hydrological indices (with F and D
quantifying the intensities of floods and droughts,
respectively), age as fixed effects, and basins and
Fish ID as random effects. We found an inverse
relationship between the increment width in the fish
hard parts and hydrological indices. That is, intense
floods and droughts negatively affected the growth
rates. We also found that the growth of P. fasciatum
was no different in years with intense and mild floods
across age classes 1--5, although was different for age
class 6. However, the growth of P. fasciatum was faster
in years of mild droughts for all age classes. Our
results showing that the growth of P. fasciatum was
slower in years of intense droughts are supported by
those of previous studies in the Amazon basin and
elsewhere. However, our results showing for the first
time that the growth of P. fasciatum is slower in years
of intense flooding is the opposite of patterns found
in other studies. These results thus suggest that the
growth of P. fasciatum is maximized within an optimum
range of hydrological conditions, where neither floods
nor droughts are intense.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Li:2024:FAB,
author = "Shaowen Li and Yufang Huang and Fan Li and Yanfen Liu
and Hongliang Ma and Xiaomin Zhang and Xiuxia Wang and
Wei Chen and Guangxin Cui and Tiantian Wang",
title = "Functional Alpha and Beta Diversity of Fish
Communities and Their Relationship with Environmental
Factors in the \geoname{Huanghe River (Yellow River)
Estuary and Adjacent Seas, China}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "222",
day = "12",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060222",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/222",
abstract = "Fisheries and bottom seawater data were collected in
July in 2022 and 2023 from 15 sampling stations in the
Huanghe River estuary and adjacent seas in China. The
patterns of functional alpha and beta diversity of fish
communities in this area and their relationships with
environmental factors were studied using trait-based
analysis. Five functional classifications, which
included 16 functional traits, four functional alpha
diversity indices (functional richness, functional
evenness, functional divergence, and community-weighted
mean), three functional beta diversity indices
(functional beta diversity and its turnover and
nestedness components), and 14 bottom environmental
factors were considered. The dominant traits of fish
communities were the following: benthivorous feeding
habits, small and front-facing mouths, high trophic
level, demersal, anguilliform, low growth coefficient,
medium resilience, low vulnerability, and adapted to
warm temperate conditions. The dominant migration
traits and types of fish eggs varied by year, and fish
abundance was the main factor affecting the dominant
traits of the communities. The results of multiple
regression on distance matrices and variance
partitioning analysis indicated that ammonia nitrogen
content, total phosphorus content, and pH were the main
environmental factors that affected fish functional
diversity. The N/P ratio had a bottom-up control effect
on fish functional diversity. Our findings also
revealed that high pH gradients and distant
geographical distances can inhibit trait turnover in
fish communities.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Robinson:2024:CTS,
author = "Wayne Robinson and John Koehn and Mark Lintermans",
title = "Contemporary Trends in the Spatial Extent of Common
Riverine Fish Species in \geoname{Australia}'s
\geoname{Murray--Darling Basin}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "221",
day = "12",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060221",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/221",
abstract = "As one of the world's most regulated river basins, the
semi-arid Murray--Darling Basin (MDB) in south-eastern
Australia is considered at high ecological risk, with
substantial declines in native fish populations already
identified and climate change threats looming. This
places great importance on the collection and use of
data to document population trends over large spatial
extents, inform management decisions, and provide
baselines from which change can be measured. We used
two medium-term data sets (10 MDB basin-wide fish
surveys from 2004--2022) covering the 23 catchments and
68 sub-catchments of the MDB to investigate trends in
the distribution of common riverine species at the
entire basin scale. Fifteen native species were
analysed for changes in their contemporary range, and
whilst short-term changes were identified, all species
showed no significant continuous trend over the study
period. We further analysed the native species extent
relative to their historic records, with bony herring
and golden perch occurring in 78\% and 68\% of their
historic river kilometres, respectively, whereas
southern pygmy perch, northern river blackfish, silver
perch, mountain galaxias, and freshwater catfish were
all estimated to occur in less than 10\% of their
historic extent. Six established non-native species
were also analysed and were very consistent in extent
over the years, suggesting that they are near the
available limits of expansion of their invasion. We
provide effect sizes for the spatial extent index which
can be used as baselines for future studies, especially
those aiming to monitor changes in the spatial extent
and population status of native species, or changes in
the spatial extent of new or existing non-native
species.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Liu:2024:HHA,
author = "Jialong Liu and Miaomiao Yin and Zhi Ye and Jingjie Hu
and Zhenmin Bao",
title = "Harnessing Hue: Advances and Applications of Fish Skin
Pigmentation Genetics in Aquaculture",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "220",
day = "10",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060220",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/220",
abstract = "Fish exhibit a broad spectrum of colors and patterns
facilitated by specialized cells known as
chromatophores. The vibrant coloration of fish,
controlled by complex genetic and environmental
interactions, serves critical roles in ecological
functions such as mating, predation, and camouflage.
This diversity not only makes fish an invaluable model
for exploring the molecular mechanisms of pigmentation
but also significantly impacts their economic value
within the aquaculture industry, where color traits can
drive marketability and breeding choices. This review
delves into the sophisticated biological processes
governing fish pigmentation and discusses their
applications in enhancing aquaculture practices. By
exploring the intersection of genetic regulation,
environmental influences, and advanced breeding
techniques, this review highlights both the scientific
understanding and practical applications of fish
coloration, providing a bridge between basic biological
research and its application in commercial
aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Barbacariu:2024:EUG,
author = "Cristian-Alin Barbacariu and Lenu{\c{t}}a
D{\^\i}rvariu and Dana Andreea {\c{S}}erban and
Cristina Mihaela R{\^\i}mbu and Cristina Elena Horhogea
and Gabriela Dumitru and Elena Todira{\c{s}}cu-Ciornea
and Constantin Lungoci and Marian Burducea",
title = "Evaluating the Use of Grape Pomace in
\bioname{Cyprinus carpio} Nutrition: Effects on Growth,
Biochemistry, Meat Quality, Microbiota, and Oxidative
Status",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "219",
day = "7",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060219",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/219",
abstract = "This study investigated the effects of incorporating
grape pomace (GP) into fish diets on the growth
performance, physiological parameters, and biochemical
composition of carp (Cyprinus carpio L.). A total of
180 carp, with an average initial weight of 65 g, were
reared in a recirculating aquaculture system (RAS).
They were divided into a control group (C), fed with a
diet containing 0\% GP, and two experimental groups (V1
and V2), fed with diets containing 5\% and 10\% GP,
respectively. The experiment lasted for eight weeks.
Results revealed that most growth parameters were not
significantly affected by GP inclusion, except for a
notable difference in the hepatosomatic index (HSI),
indicating an impact on liver size relative to body
weight. The biochemical analysis of carp meat showed
significant differences in moisture, protein, fat,
collagen, and salt content between the control and
experimental groups. Furthermore, the oxidative status
assessment indicated that GP supplementation modulates
oxidative stress and lipid peroxidation pathways in
carp, enhancing their antioxidant defenses and overall
health. Microbiological examination of the carp
intestinal content showed that GP inclusion in fish
diets influenced microbial parameters, particularly
affecting the abundance of aerobic germs and
Enterobacteriaceae.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Chen:2024:BCP,
author = "Zhaowen Chen and Yueping Zheng and Kaile Ji and
Yueyong Shang and Youji Wang and Menghong Hu",
title = "Blood-Chemistry Parameters Comparison among Different
Age Stages of \geoname{Chinese} Sturgeon
\bioname{Acipenser sinensis}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "218",
day = "7",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060218",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/218",
abstract = "The Chinese sturgeon (Acipenser sinensis), a
critically endangered migratory fish native to the
Yangtze River estuary, is experiencing alarming
population declines. Understanding the physiological
and biochemical profiles of this species is paramount
for its conservation. However, due to limited sample
availability, blood biochemical parameters have
remained understudied. In this study, we examined blood
chemistry in artificially cultured Chinese sturgeon
ranging from 2 to 15 years of age. Our results revealed
age-related trends: total protein (TP), albumin (ALB),
globulin (GLO), total cholesterol (CHOL), high-density
lipoprotein (HDL), low-density lipoprotein (LDL),
estrogen (E 2), testosterone (T), testosterone
undecanoate (11-KT), and red blood cell count (RBC)
increased with age, while glucose (GLU), uric acid
(UA), and serum creatinine (CREA) decreased. Levels of
C-reactive protein (CRP) declined from 3 to 7 years but
rose from 8 to 15 years. Blood parameters showed
stabilization with age, indicating enhanced resilience
and immunity. Significant alterations in parameters at
ages 2--3 and 14--15 suggest critical developmental
stages. These findings are crucial for understanding
sturgeon growth, development, migration, and
reproduction, underscoring the necessity for targeted
conservation efforts during pivotal life stages.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Cui:2024:FSR,
author = "Xiaojie Cui and Pengxiang Xu and Tao Tian and Mingyuan
Song and Xuyang Qin and Dehua Gong and Yan Wang and
Xuguang Zhang and Binbin Xing and Mingzhi Li and
Leiming Yin",
title = "Frequency-Specific Responses: The Impact of an
Acoustic Stimulus on Behavioral and Physiological
Indices in Large Yellow Croaker",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "217",
day = "7",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060217",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/217",
abstract = "This study assessed the impact of an acoustic stimulus
on the behavioral responses and physiological states of
the large yellow croaker (Larimichthys crocea). The
test fish, with an average body weight of approximately
352.81 {\pm} 70.99 g, were exposed to one hour of
acoustic stimulation at seven different frequencies:
100 Hz, 125 Hz, 160 Hz, 200 Hz, 500 Hz, 630 Hz, and 800
Hz. The aim was to delineate the specific effects of
acoustic stimulation on the behavior and physiological
indices. The results show that acoustic stimulation
significantly altered the behavioral patterns of the
large yellow croaker, predominantly manifested as
avoidance behavior towards the sound source. At a
stimulus frequency of 630 Hz, the test fish exhibited
continuous irregular motion and erratic swimming.
Physiologically, one hour of exposure to acoustic
stimulation notably affected the endocrine system. The
levels of Epinephrine and thyroxine were significantly
elevated at 200 Hz, while the cortisol levels did not
show significant differences. Additionally, the lactic
acid content significantly increased at 800 Hz, and the
blood glucose content peaked at 630 Hz. This study
discovered that sound frequencies of 200 Hz, 630 Hz,
and 800 Hz led to a significant increase in the levels
of Epinephrine, glucose, thyroid hormones, and lactate
in large yellow croaker, consequently affecting their
behavior. The changes in these physiological indicators
reflect the stress response of the large yellow croaker
in specific sonic environments, providing crucial
insights into the physiological and behavioral
responses of fish to acoustic stimuli.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Meerbeek:2024:PBS,
author = "Jonathan R. Meerbeek",
title = "Poststocking Behavior and Survival of Large Yearling
Muskellunge (\bioname{Esox masquinongy}) in Two
\geoname{Northern Iowa} Natural Lakes",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "216",
day = "6",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060216",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/216",
abstract = "Muskellunge are commonly stocked to maintain
populations throughout much of North America.
Poststocking survival is variable, and many
jurisdictions are experimenting with stocking
Muskellunge at advanced life stages (e.g., yearlings)
or sizes to promote more consistent recruitment within
populations. Yet, little is known about short-term
poststocking behavior or survival of Muskellunge in
natural lakes or if stocking larger fish results in
improved poststocking survival. Radiotelemetry was used
to evaluate the behavior and survival of large (mean of
418 mm; standard error of 3.4) spring-stocked yearling
Muskellunge in two northern Iowa natural lakes of
varying biotic and abiotic complexity. Hatchery-reared
Muskellunge were fitted with radio transmitters (n =
10--15 fish/lake), stocked in each lake with
conspecifics, and tracked either daily or weekly up to
112 days poststocking. Telemetry data were used to
evaluate dispersal and movement, depth and vegetation
use, proximity to shore, and survival. In both lakes,
fish dispersed rapidly from each stocking site.
Movement among lakes was similar and peaked during the
first week poststocking and declined thereafter.
Muskellunge were frequently located in offshore
habitats in both lakes, but both depth and vegetation
use varied among lakes and weeks poststocking, with
Muskellunge found in deeper, more vegetated habitats in
the larger, more complex natural lake. Muskellunge in
both systems had high poststocking survival
(86.7--90.0\%). The results of this study provide
managers with more information regarding yearling
Muskellunge poststocking behavior and survival and
indicate that larger fish may be a suitable alternative
for fisheries where high poststocking mortality may be
acting as a bottleneck for maintaining quality
Muskellunge fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Senathipathi:2024:DBR,
author = "Deep Nithun Senathipathi and Soottawat Benjakul and
Phutthipong Sukkapat and Matsapume Detcharoen and
Gururaj Moorthy and Jirakrit Saetang",
title = "{DNA} Barcoding Revealed Mislabeling of Imported
Seafood Products in \geoname{Thailand}",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "215",
day = "5",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060215",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/215",
abstract = "Seafood mislabeling threatens customer rights and
causes economic loss worldwide. The information on
seafood misrepresentation in Thailand is still lacking,
and the investigation and monitoring program must be
well established. This study investigated the
mislabeling status of imported seafood in Thailand
using the DNA barcoding technique. A total of 45
imported seafood products from five distributors were
included. Scientific, common, local, and market names
of seafood samples were obtained from FAO and Fishbase
databases. DNA was extracted, and PCR was performed
using a universal primer targeting the COI gene.
Species of each sample were identified with over 98\%
similarity based on COI sequence analysis. DNA sequence
revealed 11 mislabeled samples. Among substituted
species, Pangasianodon hypophthalmus and Thunnus
maccoyii were found to be endangered species according
to IUCN status. Products obtained from Brand-C showed
the highest mislabeling rate (42.85\%). The
phylogenetic analysis adopted with the TIM2+F+I+G4
model showed the sequenced DNA similar to the NCBI
database reference sequence. Overall, mislabeled
products of imported seafood were found at the rate of
24.44\%, suggesting that strict surveillance for
seafood substitution should be implemented in
Thailand.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Yin:2024:DDI,
author = "Zifeng Yin and Lingzhan Xue and Peng Song and Muhammad
Jawad and Cong Xu and Weishao Bu and Mingyou Li",
title = "\gene{Dazl} and \gene{dnd} Identify Both Embryonic and
Gonadal Germ Cells in \geoname{Chinese} Hook Snout Carp
(\bioname{Opsariichthys bidens})",
journal = j-FISHES,
volume = "9",
number = "6",
pages = "214",
day = "5",
month = jun,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9060214",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:26 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/6/214",
abstract = "In recent years, the Chinese hook snout carp (
Opsariichthys bidens) is becoming increasingly popular
due to its high nutritional value and delicious taste.
However, anthropogenic influences have caused a rapid
reduction in its population. Considering long-term
development sustainability, it is essential to conduct
research on its reproductive development. In this
study, the germ-cell related genes dazl (Obdazl) and
dnd (Obdnd) were cloned, and their expression patterns
were revealed. RT-PCR analysis indicated that Obdazl
and Obdnd were specifically expressed in gonads. In the
testis, Obdazl and Obdnd RNAs have a similar expression
pattern: their transcripts were abundant in
spermatogonia, gradually decreased, and eventually
disappeared during spermatogenesis. Early oocytes in
the ovary exhibited strong expression of Obdazl and
Obdnd RNAs and reduced remarkably after peak expression
at III oocytes. Surprisingly, Obdazl RNA was
dynamically found in perinuclear spots that finally
aggregated into the Balbiani body (Bb) in the early
oocytes. Moreover, Obdazl 3{\prime} UTR enabled the
reporter gene to be expressed stably in medaka PGCs,
suggesting that dazl was identified as the germ cell
marker in O. bidens, which may provide perspectives for
further studies on the development of PGCs in the
future.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Xiong:2024:PTU,
author = "Minsi Xiong and Zuli Wu",
title = "Practice of Territorial Use Rights in Fisheries in
Coastal Fishery Management in \geoname{China}: a Case
Study of the {Island Reefs Fishery Lease Policy} from
\geoname{Shengsi County in Zhejiang Province}",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "292",
day = "22",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070292",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/292",
abstract = "Coastal fisheries are vital for food supply,
employment, and social stability. However, overfishing
is a common problem, often attributed to open access.
Territorial Use Rights in Fisheries (TURFs) have gained
popularity as a tool to improve the sustainability of
coastal fisheries, but research on TURFs in China is
limited. This paper examines the island reef fishery
lease policy implemented in Shengsi County, Zhejiang
Province, within the Ma'an Islands National Marine
Special Reserve. The policy leases the use of island
reefs to a collective, aiming to address overfishing
and resource degradation by establishing fishing area
boundaries and limiting the number of users and usage
time. Technically, it is an application of TURFs, and
it represents a shift from traditional fishing licenses
to collective compliance. This experience has disrupted
the existing top--down fishery governance structure and
signifies a transition to a rights-based fishery
management system in China. Through a comprehensive
investigation and analysis of the policy's
implementation, this research identifies the factors
contributing to its flaws. These include the inadequate
provision of national and local government policies,
insufficient support for policy implementation from
fishery management authorities, and the limited
involvement of fishermen's organizations. To enhance
coastal fishery management, we propose that the Chinese
government should build upon existing foundations by
clarifying fishing rights through central and local
laws and policies. Additionally, there is a need to
strengthen data monitoring of coastal fisheries,
conduct multidisciplinary research to improve the
allocation mechanism of fishing rights, adopt diverse
fishery management approaches to enhance supervision
capabilities, establish a collaborative governance
mechanism, and foster coordination and cooperation
between grassroots fishermen's organizations and
government departments.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhu:2024:COL,
author = "Jian Zhu and Nanjun Hu and Yao Xiao and Xiaohong Lai
and Lingjiao Wang and Yufeng Song",
title = "Characterization of Ovarian Lipid Composition in the
Largemouth Bronze Gudgeon (\bioname{Coreius
guichenoti}) at Different Development Stages",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "291",
day = "22",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070291",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/291",
abstract = "The largemouth bronze gudgeon has experienced a sharp
drop in its natural population and has been listed as a
protected species in China. The frequent occurrence of
ovarian development obstruction from stage III to IV
has restricted artificial propagation. Due to lipids
being a crucial factor for ovarian development, this
study aimed to characterize the ovarian lipid profile
at different development stages in largemouth bronze
gudgeons. Using UPLC-MS/MS, 1353 lipids belonging to 46
subclasses were identified in ovaries from largemouth
bronze gudgeons. The results showed that glycerolipids
and glycerophospholipids were the dominant lipids
during ovarian development. Lysophosphatidyl choline
(LPC), phosphatidyl choline (PC), and
phosphatidylserine (PS), as the crucial phospholipids
for ovarian development, were significantly reduced
from stage III to IV. This may be the main cause of
ovarian development obstruction for largemouth bronze
gudgeons. Meanwhile, the enrichment analysis revealed
that lipid metabolites are present at different ovarian
development stages. Glycerophospholipid, linoleic acid,
and linolenic acid metabolism were significantly
enriched at stage IV. This study shows the complete
picture of the ovarian lipid composition profile, and
also discovers that phospholipids may be the limiting
factor for ovarian development; these findings offer a
theoretical basis for the artificial propagation and
release of the largemouth bronze gudgeon.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Blanes-Garcia:2024:XEE,
author = "Marta Blanes-Garc{\'\i}a and Zoran Marinovi{\'c} and
Marina Morini and Alain Vergnet and {\'A}kos
Horv{\'a}th and Juan F. Asturiano",
title = "Xenotransplantation of \geoname{European} Eel
(\bioname{Anguilla anguilla}) Spermatogonia in
Zebrafish (\bioname{Danio rerio}) and
\geoname{European} Sea Bass (\bioname{Dicentrarchus
labrax})",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "290",
day = "21",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070290",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/290",
abstract = "The European eel encounters challenges in achieving
sexual maturation in captivity, which has been a
concern for researchers. This study explores surrogate
broodstock technology as an alternative approach for
eel production. The present study aimed to evaluate
zebrafish and European sea bass as potential recipients
for European eel spermatogonia transplantation, given
the abundance of eel type A spermatogonia (SPGA).
Immature European eel testes were dissected and
maintained at 4 {$^\circ $}C or cryopreserved. SPGA
were obtained by dissociation of fresh or post-thawed
tissue, employing an enzymatic solution, and then
labelled with fluorescent membrane marker PKH26. SPGA
from fresh tissue were transplanted into wild-type
zebrafish larvae and triploid European sea bass larvae,
while SPGA from cryopreserved testis were transplanted
into vasa::egfp transgenic zebrafish larvae.
One-and-a-half months post-transplantation (mpt),
fluorescent donor cells were not detected in the gonads
of zebrafish or European sea bass. Molecular qPCR
analyses at 1.5 or 6 mpt did not reveal European
eel-specific gene expression in the gonads of any
transplanted fish. The findings suggest that the
gonadal microenvironments of zebrafish and European sea
bass are unsuitable for the development of European eel
spermatogonia, highlighting distinctive spermatogonial
stem cell migration mechanisms within teleost species",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Oliveira:2024:TBH,
author = "Joana Oliveira and Aires Oliva-Teles and Ana Couto",
title = "Tracking Biomarkers for the Health and Welfare of
Aquaculture Fish",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "289",
day = "19",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070289",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/289",
abstract = "Aquaculture production has been growing consistently
over the last few decades to meet the increasing animal
protein demand of the human population. However,
increased production and rearing intensities raise the
challenges of guaranteeing fish health and welfare,
which is essential to avoid losses and ensure product
quality. Biomarkers can provide insights into the
fish's nutritional, physiological, and health status,
and aid in the evaluation of early nutritional and
physiological imbalances, distress conditions, and
pathological diagnosis. The discovery and validation of
biomarkers rely mostly on the use of information
provided by different parameters, including
biochemical, metabolic, or immunologic, as well as
several omics, from genomics and transcriptomics to
proteomics and metabolomics. In this review, a summary
of the main biomarkers used in aquaculture is provided
along with an overview of the main omics technologies
available for further biomarker research. This review
also highlights the need to develop non-lethal
biomarkers that can easily and quickly be measured to
provide a prompt response to producers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Boissonnot:2024:UCL,
author = "Lauris Boissonnot and Camilla Karlsen and Thor Magne
Jonassen and Silje Stensby-Skj{\ae}rvik and Torolf
Storsul and Albert Kjartan Dagbjartarson Imsland",
title = "Understanding the Causes of Lumpfish
(\bioname{Cyclopterus lumpus}) Mortality in
\geoname{Norwegian} Hatcheries: Challenges and
Opportunities",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "288",
day = "19",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070288",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/288",
abstract = "Lumpfish are broadly used as biological sea lice
removers in Norwegian salmon farming and are mostly
produced in hatcheries. To date, there is little
systematic documentation on the mortality causes of
lumpfish in hatcheries. In the current study, data from
12 selected fish groups from four hatcheries in Norway
were collected to understand the challenges and
opportunities related to the categorization of
mortality causes of lumpfish in hatcheries. This study
indicated that a high proportion of lumpfish mortality
was categorized by the hatcheries as unspecified. When
specified, mortality was primarily assessed as due to
fin damage. Our analyses showed that mortality
categorized as fin damage correlated with the detection
of infectious agents in dead fish, suggesting that
mortality was due to infections rather than fin damage.
It was not possible to conclude whether infection with
pathogens present in the environment caused fin damage
or if injuries from aggression were a gateway for
pathogens. Furthermore, due to the lack of information
regarding production conditions, it was not possible to
assess risk factors causing eventual aggression or the
presence of pathogens. This study revealed that
mortality causality --- the chain of events leading to
death --- is mainly unclear in lumpfish hatcheries
because (1) mortality categorization does not focus on
underlying causes and (2) there is little documentation
of production conditions, making it very difficult to
relate mortality rates to external factors. The present
findings highlight that there are gaps in the
registrations made by hatcheries and that there is an
urgent need to implement standardized monitoring
protocols for lumpfish hatcheries. This will help
identify the causes of mortality and, therefore, allow
for the implementation of proper measures that will
ensure better survival.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Degani:2024:SDB,
author = "Gad Degani and Ari Meerson",
title = "Sex Differences in the Brain Transcriptomes of Adult
Blue Gourami Fish (\bioname{Trichogaster
trichopterus})",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "287",
day = "18",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070287",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/287",
abstract = "Blue gourami (gourami, Trichogaster trichopterus) is a
model for labyrinth fishes (Anabantoidei) adapted to
partial air breathing. Its reproductive endocrinology
has been extensively studied, and transcriptomic sex
differences in the gonads were described. Nevertheless,
sex differences in gene expression in non-gonadal
tissues ostensibly affected by the sex-specific
hormonal balance, e.g., the brain, are unknown. To
assess such differences, we used bulk RNA-seq to
assemble and compare polyA+ transcriptomes between
whole brains of four adult male and five adult female
gourami, in addition to other tissues (three dorsal fin
and five ovary samples) from the same female group.
While all nine brain transcriptomes clustered together
relative to the other tissues, they showed separation
according to sex. A total of 3568 genes were
differentially expressed between male and female
brains; of these, 1962 and 1606 showed lower and higher
expression in males, respectively. Male brains showed
stronger down-regulation of specific genes, which
included hormone receptors, e.g., pituitary adenylate
cyclase-activating polypeptide receptor (pacap-r1).
Among the genes with lower expression in male brains,
multiple pathways essential to brain function were
over-represented, including GABA, acetylcholine and
glutamate receptor signaling, calcium and potassium
transmembrane transport, and neurogenesis. In contrast,
genes with higher expression in male brains showed no
significant over-representation of brain-specific
functions. To measure the mRNA levels of specific
hormone receptors known from prior studies to regulate
reproductive function and behavior in gourami and to
validate RNA-seq results for these specific genes, we
performed RT-qPCR for five receptors, pacap-r1,
gonadotropin-releasing hormone 2 receptor (gnrh2r),
kisspeptin receptor 1 (gp{\alpha}r1/kiss1),
insulin-like growth factor 1 receptor (igf1r), and
membrane progesterone receptor 1 (mpr1), in the brain
RNA sample groups. Of these, pacap-r1 showed a
significant, three-fold down-regulation, while
gp{\alpha}r1/kiss1 showed a significant two-fold
down-regulation in male vs. female gourami brains. Our
results are novel in describing the suppression of
brain function-related gene expression in male, as
compared to female, gourami brains. Further research is
needed to assess the behavioral significance of this
effect and its prevalence in other vertebrate groups.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Luz:2024:USA,
author = "Ronald Kennedy Luz and Gisele Cristina Favero",
title = "Use of Salt, Anesthetics, and Stocking Density in
Transport of Live Fish: a Review",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "286",
day = "18",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070286",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/286",
abstract = "For freshwater or marine fish farming to be
successful, live fish must be transported. This can be
performed with eggs, larvae, juveniles, and adults.
Nonetheless, because of the handling, confinement, and
duration of transport, it is considered a difficult
procedure. To prevent significant physiological changes
that could result in fish mortality, it is crucial to
look for ways to reduce stress and enhance the quality
of transport water. Consequently, a comprehensive range
of research on the use of additives in water, including
salt and artificial or natural anesthetics, is
presented in this review, which also discusses
potential changes in water quality factors during
transport, such as dissolved oxygen (DO), carbon
dioxide (CO 2), pH, ammonia, and temperature. The usage
of stocking densities during transport is also covered
in this review, with consideration given to the various
recommendations for each species, fish size, and length
of transport.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Karlsen:2024:TDM,
author = "B{\aa}rd Ove Karlsen and Dhurba Adhikari and Tor Erik
J{\o}rgensen and Ida Klykken Hanssen and Truls Borg
Moum and Jarle Tryti Nordeide and Steinar Daae
Johansen",
title = "Two Distinct Maternal Lineages of Threespine
Stickleback (\bioname{Gasterosteus aculeatus}) in a
Small \geoname{Norwegian} Subarctic Lake",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "285",
day = "18",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070285",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/285",
abstract = "A subarctic Norwegian watercourse is known for its
population of threespine sticklebacks (Gasterosteus
aculeatus Linnaeus 1758) with unusual pelvic spine
polymorphism; the upper lake contains a mixture of
specimens that are normal-spined, asymmetric-spined,
and spineless. In contrast, the downstream lakes and
the nearby marine site contain only the normal spined
morph. We investigated the maternal-line genetic
structure in the watercourse using mitogenomics. A
total of 242 sticklebacks representing two lakes and a
marine site were assessed based on individual and
pooled DNA sequencing. While two distinct mitogenome
clades were detected in the upper lake (Lake
Storvatnet), only one of these clades was present in
the downstream lake. The marine site pooled DNA sample,
however, contained several mitogenome haplotypes. We
present mitogenome sequence features that include
gene-specific single nucleotide polymorphisms,
molecular phylogeny, and genetic differentiation
assessments based on pairwise comparisons of pooled
population samples from each site. The Lake Storvatnet
mitogenomes belong to two distinct Euro-North American
(ENA) clades; one of the ancestral lineages likely
corresponds to the original maternal lineage in the
watercourse, and the other was introduced more
recently. We hypothesize that the second invader
carried nuclear genomic features responsible for the
observed present-day pelvic spine polymorphism in Lake
Storvatnet.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kolanczyk:2024:CSP,
author = "Richard C. Kolanczyk and Laura E. Solem and Patricia
K. Schmieder and James M. McKim III",
title = "A Comparative Study of {Phase I} and {II} Hepatic
Microsomal Biotransformation of Phenol in Three Species
of {Salmonidae}: Hydroquinone, Catechol, and
Phenylglucuronide Formation",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "284",
day = "17",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070284",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/284",
abstract = "The in vitro biotransformation of phenol at 11
{$^\circ $}C was studied using pre-spawn adult rainbow
(Oncorhynchus mykiss) (RBT), brook (Salvelinus
fontinalis) (BKT), and lake trout (Salvelinus
namaycush) (LKT) hepatic microsomal preparations. The
incubations were optimized for time, cofactor
concentration, pH, and microsomal protein
concentration. Formation of Phase I ring-hydroxylation
and Phase II glucuronidation metabolites was quantified
using HPLC with dual-channel electrochemical and UV
detection. The biotransformation of phenol over a range
of substrate concentrations (1 to 180 mM) was
quantified, and the Michaelis--Menten kinetics
constants, Km and Vmax, for the formation of
hydroquinone (HQ), catechol (CAT), and
phenylglucuronide (PG) were calculated. Species
differences were noted in the Km values for Phase I
enzyme production of HQ and CAT, with the following
rank order of apparent enzyme affinity for substrate:
RBT {$>$} BKT = LKT. However, no apparent differences
in the Km for Phase II metabolism of phenol to PG were
detected. Conversely, while there were no apparent
differences in Vmax between species for HQ or CAT
formation, the apparent maximum capacity for PG
formation was significantly less in LKT than that
observed for RBT and BKT. These experiments provide a
means to quantify metabolic activation and deactivation
of xenobiotics in fish, to compare activation and
deactivation reactions across species, and to act as a
guide for future predictions of new chemical
biotransformation pathways and rates in fish. These
experiments provided the necessary rate and capacity
(Km and Vmax) inputs that are required to parameterize
a fish physiologically based toxicokinetic (PB-TK)
model for a reactive chemical that is readily
biotransformed, such as phenol. In the future, an
extensive database of these rate and capacity
parameters on important fish species for selected
chemical structures will be needed to allow the
effective use of predictive models for reactive,
biotransformation chemicals in aquatic toxicology and
environmental risk assessment.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zaccone:2024:PPI,
author = "Giacomo Zaccone and Doaa M. Mokhtar and Alessio Alesci
and Gioele Capillo and Marco Albano and Manal T.
Hussein and Marialuisa Aragona and Antonino German{\`a}
and Eugenia R. Lauriano and Ramy K. A. Sayed",
title = "From Proliferation to Protection: Immunohistochemical
Profiling of Cardiomyocytes and Immune Cells in Molly
Fish Hearts",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "283",
day = "16",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070283",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/283",
abstract = "Unlike adult mammalian cardiomyocytes, cardiomyocytes
in teleosts display high proliferative capacity
throughout adulthood. This study aimed to identify the
immunohistochemical profiles of cardiomyocytes and
immune cells in the hearts of Molly fish by assessing
the immunolabelling expression of key proteins involved
in cell proliferation, differentiation, and tissue
protection. The cardiac anatomy of Molly fish includes
the atrium, ventricle, and bulbus arteriosus. The
expression of SOX9, NF-{\kappa}B, myostatin, and S100
proteins in myocardial cells indicates the
proliferative features of the heart in Molly fish. The
bulbus arteriosus is characterized by collagenous
chambers and smooth muscle cells that express Ach and
iba1. The atrium of Molly fish serves as a storage unit
for rodlet cells and immune cells. Rodlet cells
displayed immunoreactivity to NF-{\kappa}B, iba1,
Olig2, Ach, and S100 proteins, suggesting their roles
in the immune response within the heart. Furthermore,
telocytes (TCs) have emerged as a significant component
of the atrium of Molly fish, expressing Ach, CD68, S100
protein, and iba1. These expressions indicate the
involvement of TCs in multiple signaling pathways that
contribute to heart architecture. This study delineates
the intricate relationship between cardiomyocytes and
innate immune cells in Molly fish.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Okeke-Ogbuafor:2024:UOA,
author = "Nwamaka Okeke-Ogbuafor and Tim Gray",
title = "Unregulated Open-Access versus Regulated Open-Access
Fishing: Stakeholders Perceptions in \geoname{Sierra
Leone}",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "282",
day = "15",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070282",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/282",
abstract = "Fish stocks are declining in marine capture coastal
fisheries in West Africa. Many observers have blamed
climate change and the system of open access for the
decline. Open-access fishing encourages too many people
to take up fishing, and the result is overfishing of
the stocks. According to such critics, this is a
classic example of Hardin's tragedy of the commons,
whereby a continuous increase in the number of users of
a natural resource eventually leads to the collapse of
the resource. The present study, which analysed
stakeholders' perceptions about their open-access
fishing in Siera Leone's marine fisheries, conducted 32
key informant interviews. Our research found that while
they perceived that open-access fishing impacted fish
stocks, most Sierra Leone's marine capture fishers did
not see open access as a cause of fish decline;
instead, they blamed the lack of enforced regulations
on the methods and extent of fishing. Accordingly,
these fishers favoured the continuation of open access
--- not least because it helps to alleviate hunger and
is a readily available source of protein in Sierra
Leone's coastal communities --- but accompanied by
strict regulations on both the type of fishing gear
used and the quantity, size, and species of fish
caught. In other words, they preferred regulated open
access to unregulated open access. The central
government's resolve to regulate its fishery will
determine whether it will transition from unregulated
open access to regulated open access or other forms of
OA. This study recommends detailed research into how to
influence political will to enforce regulations.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2024:CSC,
author = "Yanping Zhang and Haixin Zhang and Zijun Wu and
Mingguang Zhao and Guangpeng Feng",
title = "Community Structure Characteristics and Changes in
Fish Species at \geoname{Poyang Lake} after the
\geoname{Yangtze River} Fishing Ban",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "281",
day = "15",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070281",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/281",
abstract = "To understand the fish community structure
characteristics and changes in Poyang Lake after the
Yangtze River fishing ban in 2021, seven sampling
stations were established, namely in Hukou, Lushan,
Duchang, Yongxiu, Nanjishan, Ruihong, and Poyang, for
fishery resource surveys between 2020 and 2021. The
results showed that 93 fish species were identified,
belonging to 11 orders, 20 families, and 61 genera,
which was an increase of 4.5\% from before the fishing
ban. Of these, 52 Cyprinidae species were identified,
comprising the highest proportion (55.91\%). Four
invasive alien species (hybrid sturgeon, Cirrhina
mrigala, Piaractus brachypomus, and Mugil cephalus)
were identified, and the number of alien species in
Poyang Lake was higher than before the fishing ban.
Carnivorous and mid-lower-level fish showed a
significant increase, accounting for 47.31\% and
38.71\% of the total species, respectively. Compared
with the Yangtze River before the fishing ban, the body
length and body weight of the main economic fish in
Poyang Lake increased by 6.10--61.26\% and by
15.14--291.57\%, respectively. In terms of age
structure, the proportion of major economically
important fish aged 1 or 2 years decreased
significantly, while the proportion of older fish
increased substantially. There was little difference in
the diversity of fish communities at different stations
in Poyang Lake. In terms of biodiversity, the
Shannon--Wiener index ranged from 2.158 to 2.909, with
Poyang having the highest value and Nanjishan the
lowest. Margalef's index ranged from 4.265 to 6.459,
with Lushan having the highest value and Nanjishan the
lowest. Pielou's index ranged from 0.617 to 0.822, with
Duchang having the highest value and Nanjishan the
lowest. Hence, the Yangtze river fishing ban has played
an important and positive role in the restoration of
fishery resources in Poyang Lake. However, long-term
tracking and monitoring are needed to provide
information to comprehensively evaluate the ecological
impact of the Poyang Lake fishing ban.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Bergstrom:2024:SDM,
author = "Kristofer Bergstr{\"o}m and Hanna Berggren and Oscar
Nordahl and Per Koch-Schmidt and Petter Tibblin and Per
Larsson",
title = "Seasonal and Daily Movement Patterns by {Wels} Catfish
(\bioname{Silurus glanis}) at the Northern Fringe of
Its Distribution Range",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "280",
day = "14",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070280",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/280",
abstract = "Fish behavior often varies across a species'
distribution range. Documenting how behaviors vary at
fringes in comparison to core habitats is key to
understanding the impact of environmental variation and
the evolution of local adaptations. Here, we studied
the behavior of Wels catfish (Silurus glanis) in Lake
M{\"o}ckeln, Sweden, which represent a European
northern fringe population. Adult individuals (101--195
cm, N = 55) were caught and externally marked with data
storage tags (DSTs). Fifteen DSTs were recovered a year
after tagging, of which 11 tags contained long-term
high-resolution behavioral data on the use of vertical
(depth) and thermal habitats. This showed that the
catfish already became active in late winter ({$<$5}
{$^\circ $}C) and displayed nocturnal activity
primarily during summer and late autumn. The latter
included a transition from the bottom to the surface
layer at dusk, continuous and high activity close to
the surface during the night, and then descent back to
deeper water at dawn. During the daytime, the catfish
were mainly inactive in the bottom layer. These
behaviors contrast with what is documented in
conspecifics from the core distribution area, perhaps
reflecting adaptive strategies to cope with lower
temperatures and shorter summers.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{AmorosoFerrari:2024:CHE,
author = "Nat{\'a}lia {Amoroso Ferrari} and Leonardo {Mantovani
Favero} and Cesar {Toshio Facimoto} and Alais Maria
{Dall Agnol} and Marcos {Letaif Gaeta} and Thalita
{Evani Silva de Oliveira} and Daniela Dib
Gon{\c{c}}alves and Nelson Maur{\'\i}cio Lopera-Barrero
and Ulisses de P{\'a}dua Pereira and Giovana {Wingeter
Di Santis}",
title = "Clinical and Histopathological Evolution of Acute
Intraperitoneal Infection by \bioname{Streptococcus
agalactiae} Serotypes {Ib} and {III} in \geoname{Nile}
Tilapia",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "279",
day = "13",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070279",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/279",
abstract = "Streptococcus agalactiae is a highly invasive
bacterium that causes significant economic losses in
tilapia aquaculture around the world. Furthermore, it
is a pathogen for mammals, including humans,
emphasizing its importance in One Health. The aim of
this work was to evaluate the evolution of clinical and
histopathological lesions caused by acute infection
with two serotypes of S. agalactiae. For this, two
strains isolated from natural outbreaks in Brazilian
aquaculture farms (S13, serotype Ib; S73, serotype III)
were used to challenge juvenile Nile tilapia (
Oreochromis niloticus) intraperitoneally. Target organ
samples were collected ten times, between 1 and 96 h
post-infection, for microbiological and
histopathological analyses. Anorexia was the first
clinical sign and the first death occurred at 24 and 30
h in the fish infected with strains S13 and S73,
respectively. Serotype Ib initially caused more
pronounced lesions in the nervous system; however,
serotype III lesions progressed more aggressively,
reaching the same severity as those of serotype Ib.
This trend was repeated in the mortality curve after 32
h. These results elucidated the important stages in the
pathogenesis of S. agalactiae serotypes Ib and III in
tilapia and suggest ``tips and tricks'' to improve the
positive culture rate in the clinical diagnosis of
infections in some tissues.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Petriki:2024:UGI,
author = "Olga Petriki and Dimitra C. Bobori",
title = "Unraveling \geoname{Greek} Inland Competitive Fishing:
Historical Insights, Angler Profiles, and Motivations
through Limited Data Integration in Recreational
Fishing Research",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "278",
day = "13",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070278",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/278",
abstract = "This study delves into the realm of competitive sport
fishing in Greece's inland waters, where recent years
have witnessed a notable surge in such activities.
Despite the absence of official records, insights into
the sport's trend and the motivations and
socio-demographic characteristics of anglers
participating in fishing competitions were gleaned from
digital data sourced from online platforms and
interviews with anglers. Our findings illuminate the
growing popularity of sport fishing in Greece, evident
in the increasing number of fishing competitions and
the burgeoning angling community. Predominantly male,
anglers exhibit a strong dedication to
catch-and-release practices, citing relaxation and
socialization as primary motivations, with competitive
aspirations also shaping their involvement. Moreover,
the study highlights legislative gaps in inland water
fisheries regulations, underscoring the need for
updates to ensure sustainable management and to
mitigate illegal activities. Additionally, there exists
an opportunity to bolster anglers' ecological awareness
through targeted education initiatives. Ultimately,
addressing these conclusions holds the potential to
foster responsible fishing practices and contribute to
the long-term sustainability of inland water
fisheries.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Badouvas:2024:FHP,
author = "Nicholas Badouvas and Konstantinos Tsagarakis and
Stylianos Somarakis and Paraskevi K. Karachle",
title = "Feeding Habits and Prey Composition of Six Mesopelagic
Fish Species from an Isolated Central
\geoname{Mediterranean} Basin",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "277",
day = "12",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070277",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/277",
abstract = "Mesopelagic fishes hold an important position in
marine food webs, serving as a link between lower
trophic levels and top predators and transferring
energy from their deep mesopelagic habitat to shallower
oceanic layers. Despite their ecological importance,
research on mesopelagic fishes' diet and feeding habits
in the Mediterranean Sea is far from thorough. The
present work attempts to assess the preying patterns
and diet composition of four myctophid (Benthosema
glaciale, Ceratoscopelus maderensis, Myctophum
punctatum,~Notoscopelus~elongatus) and two
sternoptychid (Argyropelecus hemigymnus,~Maurolicus
muelleri) species from the Corinthian Gulf (Ionian Sea,
Greece), sampled during pelagic trawl surveys in 2018
and 2019. Stomach vacuity was high for myctophids
caught during daytime, a pattern which sternoptychids
did not follow. Estimated trophic indices revealed high
dietary diversity (Shannon's H ' index) for most
investigated species, but a narrow trophic niche
breadth (Levins' normalized B n index). Copepods and
various marine crustaceans were dominant in all diets,
classifying them under the zooplanktivorous trophic
guild, while A. hemigymnus exhibited high
concentrations of particulate organic matter in their
stomachs and N. elongatus exhibited consumption of
fish. Diet overlap was significant among most studied
mesopelagic species, as indicated by Shoener's S index
and confirmed by both the multidimensional scaling
ordination and a hierarchical cluster analysis.
Information on mesopelagic fishes' diet composition in
this poorly studied part of the Mediterranean is useful
in further assessing and parameterizing marine food
webs and midwater trophic interactions, as well as in
quantifying the ensued energy transfer to top predators
of commercial interest or conservation concerns.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhang:2024:MCC,
author = "Yang Zhang and Jiayuan Shi and Yuntao Lu and Qing Luo
and Pengfei Chu and Rong Huang and Kunci Chen and Jian
Zhao and Yaping Wang and Mi Ou",
title = "Molecular Cloning and Characterization of Scavenger
Receptor Class {B} Type 1 in Grass Carp
(\bioname{Ctenopharyngodon idellus}) and Its Expression
Profile following Grass Carp Reovirus Challenge",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "276",
day = "12",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070276",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/276",
abstract = "As a member of the pattern recognition receptor (PRR)
class, scavenger receptor class B type 1 (SRB1) plays a
key role in innate immunity. Grass carp (
Ctenopharyngodon idellus) ranks among the most
extensively cultivated freshwater aquaculture species
in China. However, little is known about the function
of SRB1 in C. idellus. In this research study, a SRB1
gene was identified in C.~idellus, named CiSRB1. The
full-length cDNA of CiSRB1 is 2486 bp long, with an
open reading frame (ORF) of 2486 bp encoding a 497
amino acid (aa) protein containing a conserved CD36
domain. The identified genomic DNA length of CiSRB1 is
20,042 bp, including 12 exons and 11 introns. The
predictive analysis of protein interactions revealed
that Ci SRB1 could interact with the outer capsid
proteins of typical GCRV strains. The tissue
distribution of CiSRB1 exhibited age-dependent
characteristics. CiSRB1 displayed the highest
expression in the intestines and moderate levels in
muscle, spleen, liver, and brain of one-year-old grass
carp while maintaining relatively low levels in
three-year-old grass carp. Following grass carp
reovirus (GCRV) infection, notable upregulation of
CiSRB1 transcripts was observed in major immune tissues
(gills, intestines, spleen, and liver). Furthermore,
significant differences were found between one-year-old
and three-year-old grass carp, with lower CiSRB1
expression levels being detected in the older group.
Additionally, a distinct response to GCRV infection was
observed in one-year-old and three-year-old grass carp.
It was found that one-year-old individuals had a
mortality rate of up to 84\% 6 days post-infection
(dpi), whereas all three-year-old counterparts survived
after GCRV infection. The analysis of GCRV copy numbers
across tissues revealed substantially higher levels in
one-year-old grass carp compared with their older
counterparts, confirming the existence of age-dependent
susceptibility to GCRV infection in grass carp.
Combined with these results, it was speculated that the
decline in cell-surface CiSRB1 expression with age may
impede reovirus binding to host cells, potentially
explaining why older grass carp demonstrated enhanced
resistance to GCRV infection. This observation
accentuates the importance of CiSRB1 in the context of
GCRV infection and provides insights into age-dependent
susceptibility to reovirus.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Han:2024:ERA,
author = "Qianyun Han and Jiajie Hu and Weicong Pan and Jin Yu
and Xiaoguo Ying and Jinpeng Weng and Weiye Li and
Xudong Weng",
title = "Effects of Rewilding Aquaculture Time on Nutritional
Quality and Flavor Characteristics of Grass Carp
(\bioname{Ctenopharyngodon idellus})",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "275",
day = "11",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070275",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/275",
abstract = "Wild fish are preferred by consumers primarily for
their superior sensorial qualities, including taste and
texture. However, their limited availability often
results in higher prices. Considering this, we explored
the possibility of enhancing the quality of earthen
pond aquaculture fish by transferring them to a near
wild environment. This study investigated how rewilding
time affects the physical properties, nutritional
composition, and volatile profile of grass carp muscle.
The results showed that compared to the 0M group, the
crude protein content in grass carp muscle did not
change significantly (p {$>$} 0.05) as the rewilding
time increased to 6 months. Meanwhile, the significant
increase in hardness and springiness (p {$<$} 0.05)
indicated that the textural characteristics of muscle,
which were key sensory and physical indices of muscle
quality, were improved. Although the 6M group showed a
58.93\% reduction in crude fat content compared to the
0M group, it retained the highest docosahexaenoic acid
(DHA) content. Sensory evaluation demonstrated that as
the rewilding time increased, the fishy and grassy
odors of the rewilding grass carp diminished.
Furthermore, cluster heatmaps and partial least squares
discriminant analysis (PLS-DA) revealed that cultured
grass carp and rewilding grass carp at three time
points exhibited differences in various indicators. The
variable importance in projection (VIP) showed that
volatile flavor compounds (acetone, propionaldehyde-D,
1-penten-3-ol) and hardness were key factors in
distinguishing between them. Therefore, extending the
rewilding time can potentially enhance the
acceptability of cultured grass carp by improving the
physical properties, nutritional quality, and volatile
profile of the muscle. This approach may provide a new
pathway for fish aquaculture.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Damir:2024:WFM,
author = "Nicoleta Damir and Valentina Coatu and Diana Danilov
and Luminita Laz{\u{a}}r and Andra Oros",
title = "From Waters to Fish: a Multi-Faceted Analysis of
Contaminants' Pollution Sources, Distribution Patterns,
and Ecological and Human Health Consequences",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "274",
day = "11",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070274",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/274",
abstract = "This study presents an extensive evaluation of the
contamination levels in fish, mollusks, water, and
sediments in the Black Sea over eight years, from 2016
to 2023. The primary aim was to determine the
concentrations and distribution patterns of heavy
metals (HMs), polychlorinated biphenyls (PCBs),
polycyclic aromatic hydrocarbons (PAHs), and other
persistent organic pollutants (POPs) in fish, water,
and sediments of the Black Sea, and their implications
for marine ecosystem health and human safety. Data were
collected through scientific cruises and the
methodology involved systematic sampling across
different regions of the Romanian Black Sea, followed
by rigorous laboratory analyses to identify and
quantify the presence of contaminants. The study also
examined the temporal trends of these pollutants,
providing insights into their sources, pathways, and
persistence in the marine environment. Additionally,
the research assessed the bioaccumulation of
contaminants in various biota, offering a critical
perspective on food safety and potential risks to human
consumers. The findings revealed significant spatial
insights, highlighting areas of concern that require
immediate attention and action. Notably, industrial
discharge, agricultural runoff, and historical
pollution hotspots were identified as major sources of
contamination. This research underscores the need for
enhanced monitoring and regulatory frameworks to
mitigate pollution sources and safeguard the Black Sea
ecosystem, advocating for sustainable practices and
effective management strategies to preserve marine
resources in the Black Sea.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Marteinson:2024:DRV,
author = "Sarah Marteinson and Melanie Kingsbury and Dounia
Hamoutene",
title = "Data Review on the Variation in Sensitivity to
Aquaculture Chemotherapeutants in Some Crustacean Life
Stages",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "273",
day = "11",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070273",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/273",
abstract = "Aquaculture chemotherapeutants used to control sea
lice in finfish aquaculture can disperse into the
marine habitat and have the potential to affect
non-target species like crustaceans. Some of the
compounds used (especially in Canada) include in-feed
drugs such as emamectin benzoate (EMB), and ivermectin
(IVER), as well as bath pesticides such as azamethiphos
(AZA) and hydrogen peroxide (HP). Despite the paucity
of data, crustacean life cycle stages appear to have
varying sensitivities to these compounds. This data
review sets out to examine the intraspecies variation
in sensitivity within life stages for lobster (Homarus
) and prawn (Pandalus) species. Despite limited
information, it appears that prawn eggs, adults, and
juveniles are less sensitive to AZA and EMB compared
with larvae, whereas for lobster the opposite is true:
adults and juveniles are more sensitive than larvae to
AZA (there was insufficient data to assess EMB). For
HP, the sensitivity of prawn eggs appears comparable to
larvae, but hatching success data suggest that this
important stage of development is less impacted than
eggs themselves as indicated by one study. These
differences are important considerations for toxicity
threshold determination processes and risk assessments,
which ideally are environmentally relevant, and
highlight the need for more data.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhai:2024:EWF,
author = "Cunhua Zhai and Yutao Li and Guanyu Zhu and Wenjie
Peng and Qiuxu E and Ying Zhang and Bo Ma",
title = "The Effects of Water Flow Speed on Swimming Capacity
and Energy Metabolism in Adult \geoname{Amur} Grayling
(\bioname{Thymallus grubii})",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "272",
day = "10",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070272",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/272",
abstract = "The present study aimed to explore whether water flow
velocity could affect the swimming ability and overall
energy metabolism of wild Amur grayling (Thymallus
grubii). Swimming performance was assessed by measuring
critical swimming speed (U crit), burst speed (U
burst), and oxygen consumption rate (MO 2) based on the
stepped velocity test method. Our results showed that
the absolute values of U crit and U burst tended to
increase with body length. In contrast, the relative
values of U crit and U burst tended to decrease and
increase, respectively. MO 2 in Amur grayling was
elevated with increasing velocity, suggesting
relatively high swimming efficiency. We also measured
the biochemical indices related to energy metabolism.
Lactate dehydrogenase, hexokinase, and pyruvate kinase
activities significantly increased (p {$<$} 0.05).
Hepatic glycogen, glucose, and muscle glycogen contents
decreased with the increasing trend of velocity (p
{$<$} 0.05), the lactic acid contents of the blood and
muscles increased significantly with the increase in
velocities (p {$<$} 0.05), and changes in creatine
phosphate content showed no significant difference (p
{$>$} 0.05). The results not only denote the
relationship between body size and swimming speed but
also show the effects of water flow velocity on energy
metabolism in Amur grayling. The results provide basic
data for the construction of fish passage.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Kempf:2024:HTR,
author = "Sarah Kempf and Heidrun Sigrid Windisch and Kristina
Lore Kunz and Hans-Otto P{\"o}rtner and Felix
Christopher Mark and Magnus Lucassen",
title = "Hepatic Transcriptomic Responsiveness of Polar Cod,
\bioname{Boreogadus saida}, to Ocean Acidification and
Warming",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "271",
day = "10",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070271",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/271",
abstract = "Background: This study was part of a larger
comprehensive project (BIOACID) addressing the
physiological resilience of Polar cod, Boreogadus
saida, to ocean acidification and global warming and
aimed to unravel underlying molecular mechanisms of the
observed physiological responses. Methods: Fish were
acclimated long-term to three CO 2 concentrations
comprising control conditions (390 ppm) and two
projected climate scenarios (780 ppm and 1170 ppm).
Each CO 2 treatment was combined with four
temperatures: 0, 3, 6, and 8 {$^\circ $}C. Here, we
focused on the hepatic transcriptomic profiles from
these previously physiologically characterized fish.
Results: Generally, we did not detect signs of a
classical stress response. Consistent with functional
observations, warming induced much stronger molecular
responses compared to elevated P CO 2, but an
interaction between both factors existed to some
extent. Gene ontology analysis revealed a strong
response in lipid, amino acid, and protein metabolism.
With increasing temperature, we observed a shift away
from lipid metabolism, while carbohydrate metabolic
pathways remained stable. Conclusions: Although we
found Polar cod to be quite resilient to ocean
acidification, temperature will remain a critical
parameter for this valuable Arctic keystone species,
and the question remains as to whether the observed
acclimation strategies can be implemented in its
natural habitat, especially when food supply is
limited.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Ulman:2024:IPC,
author = "Aylin Ulman and Cat A. Gordon and Ali R. Hood and
Melanie Warren and Daniel Pauly",
title = "Identifying Potential Critical Angel Shark Areas in
\geoname{T{\"u}rkiye, Eastern Mediterranean} Based on
New Records of \bioname{Squatina} spp. Identified
through Fisher Engagement",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "270",
day = "8",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070270",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/270",
abstract = "This study presents new records of three Critically
Endangered angel shark species (Family: Squatinidae)
occurring in the Eastern Mediterranean --- Smoothback
Angelshark S. oculata Bonaye, 1840, Sawback Angelshark
S. aculeata Cuvier, 1829, and Angelshark S. squatina
(Linnaeus, 1758). The supporting data serves to
highlight three potential Critical Angel Shark Areas
(CASAs) in T{\"u}rkiye: Fethiye Bay, {\c{C}}anakkale
Strait (i.e., Dardanelles), and Antalya Bay. These data
also demonstrate that female S. oculata may mature at a
smaller size than prior published estimates of length
at first maturity. This new dataset provides details of
23 S. squatina specimens, 52 S. oculata specimens, and
5 S. aculeata specimens, totalling 80 recent angel
shark specimens found in Turkish waters mostly sent to
us from small-scale fishers who had incidentally caught
angel sharks. Also presented are four capture-induced
parturition events in Turkish waters onboard fishing
vessels, thus providing details on internal yolk sacs,
reproductive habitats, and indications of spawning
season. Our dataset presented here spans from 2018 to
2023 and suggests that mature adults of S. squatina and
S. oculata still occur in Turkish waters, in Fethiye
Bay and {\c{C}}anakkale, respectively. Due to elevated
chances of fishers encountering Critically Endangered
angel sharks in T{\"u}rkiye, we suggest that fishers
are trained in handling and safe-release methods, to
ensure reduced incidents of capture-induced
parturition, and improved post-release survival. This
study demonstrates that bottom set nets set by
small-scale fishers pose less risk to mortality for
angel sharks as they are almost always encountered
alive after a usual 12 h soak time, suggesting that
bottom trawling in critical habitats should be better
regulated (or phased out) to help improve national
angel shark conservation initiatives.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Pan:2024:EEH,
author = "Yucai Pan and Gefeng Xu and Tianqing Huang and Wei Gu
and Enhui Liu and Gaochao Wang",
title = "Effects of Exogenous Hormones on Spawning
Performances, Serum Gonadotropin and Sex Steroid
Hormone in \geoname{Manchurian} Trout
(\bioname{Brachymystax lenok}) during Sexual
Maturation",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "269",
day = "8",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070269",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/269",
abstract = "The objective of this study was to investigate the
effects of salmon gonadotropin releasing hormone analog
(S-GnRH-A) and human chorionic gonadotropin (HCG) on
the serum levels of gonadotropins (GtHs) and sex
steroid hormones in Manchurian trout during sexual
maturity. Females in the treatment group were injected
with 20 {\SGMLmicro}g/kg S-GnRH-A and 400 IU/kg HCG,
whilst males were injected with half the amount. Blood
samples were collected at 0, 5, 10, and 20 days post
injection (dpi). The results showed an increase in
female follicle-stimulating hormone (FSH) levels in the
treatment group at 5 dpi, and from 5 dpi onwards, a
difference was observed between the groups, with higher
FSH levels in the treatment group compared to the
control group. In males, FSH levels showed group
differences at only 5 dpi (p {$<$} 0.05) and were
higher in the treatment group. In females, luteinizing
hormone (LH) peaked at 10 dpi in the treatment group,
and there were differences between the two groups at 10
dpi and 20 dpi. In males, LH was significantly higher
in the treatment group than in the control group at 5
dpi and 10 dpi (p {$<$} 0.05). In females, estradiol
(E2) was significantly higher in the treatment group
than in the control group at 5 dpi and 10 dpi (p {$<$}
0.05), whereas in males, the difference between the two
groups was only observed at 5 dpi (p {$<$} 0.05). The
female testosterone (T) level in the treatment group
was significantly higher than that of the control group
at 5 dpi and 10 dpi (p {$<$} 0.05), while T levels were
significantly higher in the male treatment group than
in the control group at 5 dpi (p {$<$} 0.05). The level
of 17{\alpha}--hydroxyprogesterone (17{\alpha}-OHP) in
the females of the treatment group was significantly
higher than that of the control group at 5 dpi and 10
dpi (p {$<$} 0.05), and 17{\alpha}-OHP levels were
significantly higher in the male treatment group than
in the control group at 10 and 20 dpi (p {$<$} 0.05).
The fish were successfully spawned after S-GnRH-A and
HCG injections, and brood amount, fertilization rate,
and hatching rate were significantly increased (p {$<$}
0.05). This study provides a framework for a better
understanding of the mechanisms of exogenous
hormone-mediated control of reproduction in Manchurian
trout.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Calderon-Garcia:2024:EZE,
author = "Andres Angel Calderon-Garcia and Mercedes
S{\'a}nchez-Barba and Veronica Gonzalez-Nunez",
title = "Exposure of Zebrafish Embryos to Morphine and Cocaine
Induces Changes in the Levels of Dopamine and of
Proteins Related to the Reward Pathway",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "268",
day = "5",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070268",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/268",
abstract = "Morphine, a drug of abuse used to treat
moderate-to-severe pain, elicits its actions by binding
to the opioid receptors. Cocaine is an example of a
recreational drug that inhibits dopamine reuptake. The
molecular effects of morphine and cocaine have been
described in different specific brain regions. However,
the systemic outcome of these drugs on the whole
organism has not been fully addressed. The aim of this
study is to analyse the global effects of morphine (10
{\mu}M) and cocaine (15 {\mu}M) in the expression of
proteins related to the reward pathway. Zebrafish
embryos were exposed to these drugs from 5 hpf (hours
post-fertilisation) to 6 dpf (days post-fertilisation).
Dopamine levels were determined by ELISA, and the
expression of Fos proteins, Creb, its activated form
p-Creb and tyrosine hydroxylase (Th) were examined by
Western blot. Both drugs decreased Th levels at 72 hpf
and 6 dpf and modified the expression of Fos family
members, pCreb and Creb in a time-dependent manner.
Morphine and cocaine exposure differentially modified
dopamine levels in 72 hpf and 6 dpf zebrafish embryos.
Our results indicate that drugs of abuse modify the
expression of several proteins and molecules related to
the activation of the reward pathway.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Huang:2024:MAT,
author = "Xianpeng Huang and Zhenlin Hao and Junxia Mao and Luo
Wang and Xubo Wang and Ying Tian",
title = "Morphometric Analysis of Two Shell Color Strains of
the Bay Scallop \bioname{Argopecten irradians}",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "267",
day = "5",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070267",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/267",
abstract = "In this study, we employed geometric morphometrics
(GMs) to analyze the shell shape differences among two
mass-selected strains of bay scallops (red shell strain
(RS) and black shell strain (BS)) and a control group
(CG). The results revealed distinct shell shape
differences corresponding to shell coloration, with the
black shell strain displaying a more elliptical shell
compared to the red shell strain. Additionally, the
umbonal angle of the red shell strain was smaller than
that of the black shell strain, indicating that the
black shell strain had a more favorable jet direction
that could enhance swimming capabilities. To evaluate
the model's performance in practical applications,
leave-one-out cross-validation was carried out on the
two shell strains and one control group. The results
demonstrated discrimination accuracy rates of 67.44\%,
47.62\%, and 68.18\% for the BS strain, RS strain, and
CG, respectively. Similarly, for the right valves, the
discrimination accuracy rates were 62.79\%, 50.00\%,
and 75.00\% for the BS strain, RS strain, and CG,
respectively.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Jiang:2024:IDD,
author = "Yiying Jiang and Lei Huang and Yang Liu and Shuang
Wang",
title = "Impact of Digital Development and Technology
Innovation on the Marine Fishery Economy Quality",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "266",
day = "5",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070266",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/266",
abstract = "The digital economy plays an important role in
promoting the high quality and sustainable development
of the marine fishery economy. Based on the panel data
of the digital economy and marine fishery development
from 2011 to 2022, we firstly adopted the entropy
method to comprehensively evaluate the economy quality
level of the digital economy and marine fishing.
Secondly, we constructed a two-way fixed effect model
to empirically analyze the impact of digital economy
development on the marine fishery economy quality and
the mediating role of marine green science and
technology innovation, and further explored the
regional heterogeneity of the digital economy on the
marine fishery economy quality. Several findings
emerge. The digital economy and the marine fishery
economy quality level was relatively low and showed a
fluctuating increase. The development of the digital
economy can significantly improve the economy quality
of marine fishing, and the conclusion was still valid
after considering a series of robustness tests. The
digital economy can drive the development quality of
the marine fishery economy through marine green
technology innovation. This paper proposes fostering
the advancement of digital technology in the marine
fishery sector, to effectively harness the
innovation-driven potential of the digital economy, so
as to facilitate the harmonious development of both the
marine and digital economies.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Lai:2024:DFR,
author = "Xiaojian Lai and Shuai Peng and Zhaoren Bai and Le Cao
and Huixuan Huang and Yonghua Jiang and Yilei Wang",
title = "Direct Feedback Regulation of {E2}, T, and {hCG} in
the Brain--Pituitary--Gonad Axis of \geoname{Japanese}
Eel (\bioname{Anguilla japonica}) during Artificial
Maturation",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "265",
day = "5",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070265",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/265",
abstract = "The feedback regulatory effects of estrogen (E2) and
androgen (T) on the gonadotropin-releasing hormone
(GnRH) and gonadotropin (GtH) within the
brain--pituitary--gonad (BPG) axis in eels with
undeveloped ovaries were investigated through in vivo
studies. However, the regulatory role of the BPG axis
only became apparent during ovary development in the
migratory stage. To further elucidate the direct
feedback regulation of the BPG axis, female Anguilla
japonica underwent artificial induction of
vitellogenesis, and the regulation of BPG axis tissues
by GtH (human chorionic gonadotropin, hCG), E2, and T
was explored through in vitro exposure. The mRNA
expression levels of GnRH (mGnRH), GtH (fshb and lhb ),
and steroid biosynthesis enzymes (cyp11a1, hsd3b,
cyp17a1, and cyp17a2) in the diencephalon, pituitary,
and ovary, respectively, were determined. The results
showed that the expression level of mGnRH in the
diencephalon was significantly downregulated by 0.1
IU/mL hCG but upregulated by both 1 nM E2 and higher
concentrations of T, suggesting a direct positive
feedback regulation of E2 on mGnRH. In the pituitary,
the expression levels of fshb and lhb were upregulated
by E2, while fshb was suppressed by T. In the ovaries,
the expression of cyp11a1 and hsd3b was upregulated by
1 nM E2, whereas T exposure resulted in an opposite
effect. Cyp17a1 mRNA levels did not differ
significantly with E2 treatment but were upregulated by
1 nM T. These findings suggest that low concentrations
of E2 exhibited positive feedback regulation on all
three levels (diencephalon, pituitary, and ovary) of
the BPG axis, while T showed weaker and differential
feedback regulation in BPG axis tissues. Overall, this
study's results revealed the direct feedback regulation
of hCG, E2, and T on the BPG axis in eels, a
phylogenetic base of teleosts.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Amin:2024:SDM,
author = "Muhamad Amin and Hussein Taha and Laila Musdalifah and
Muhamad Ali and Alimuddin Alimuddin and Sahrul Alim and
Takaomi Arai",
title = "Structure and Diversity of Microbiomes Associated with
the Gastrointestinal Tracts of Wild Spiny Lobsters and
Profiling Their Potential Probiotic Properties Using
{eDNA} Metabarcoding",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "264",
day = "4",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070264",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/264",
abstract = "Microbial communities have been documented as playing
many pivotal roles, and contributing to the growth or
health performance of animal hosts. Thus, many studies
are currently looking for potential beneficial bacteria
``probiotics'' from diverse environments, including
wild species. The present study aimed to investigate
the diversity and potential metabolic functions of
bacterial communities in the gastrointestinal tract of
wild spiny lobsters. The gastrointestinal (GI) tracts
of two wild lobster species (Panulirus ornatus and
Panulirus homarus) were dissected aseptically and
analyzed through high-throughput sequencing, followed
by PICRUSt analysis. The results exposed that the most
dominant phyla inhabiting both lobster species at the
post-puerulus and juvenile stages were Proteobacteria,
Firmicutes, Bacteriodota, Patescibacteria, and
Verrucomicrobiota, while at the genus level, the GI
tracts were mostly dominated by Photobacterium,
Candidatus Bacillopora, Vibrio, and Catenococcus at the
post-peurulus stage, and Vibrio, Catenococcus,
Acanthopleuribacter, Acinetobacter, Pseudoalteromonas,
Grimontia, and Photobacterium at the juvenile stage.
Further metagenomic prediction analysis discovers many
potential probiont properties indicated by the
detection of marker genes corresponding to many
important metabolic activities, such as antimicrobial
compounds (streptomycin, vancomycin, carbapenem,
tetracycline, novobiocin, penicillin, cephalosporin,
ansamycines, butirosin, and neomycin), antioxidants
(e.g., flavonoids and carotenoids), and several
important digestive enzymes (e.g., lipase, protease,
and amylase). These results suggest that GI tracts of
wild spiny lobsters are potential sources to discover
novel probionts for aquaculture purposes. Further
studies, such as the isolation of the natural
product-producing bacteria, or cloning of the
beneficial compound-identified genes, are highly
recommended to develop novel probiotic strains for
aquaculture purposes.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
@Article{Zhong:2024:FSB,
author = "Botao Zhong and Huaihua Yu and Shengming Han and
Weiwei Song and Zhiming Ren and Chunlin Wang and
Changkao Mu",
title = "Functional Study on the {BMP} Signaling Pathway in the
Molting of \bioname{Scylla paramamosain}",
journal = j-FISHES,
volume = "9",
number = "7",
pages = "263",
day = "4",
month = jul,
year = "2024",
CODEN = "????",
DOI = "https://doi.org/10.3390/fishes9070263",
ISSN = "2410-3888",
bibdate = "Tue Aug 20 10:28:27 MDT 2024",
bibsource = "https://www.math.utah.edu/pub/tex/bib/fishes.bib",
URL = "https://www.mdpi.com/2410-3888/9/7/263",
abstract = "In this study, we added LDN-193189 2HCL to inhibit the
BMP signaling pathway in Scylla paramamosain and then
explored the function of this pathway in molting
through the changes in the growth performance and
molt-related gene expression. The study findings
indicated that the expression of ACVR1, BMPRIB, and
Smad1 in Scylla paramamosain was suppressed when the
LDN-193189 2HCL concentration in the culture water was
2 {\SGMLmicro}m/L. Subsequently, following a 30-day
experiment, there was a significant reduction in the
molting frequency, growth rate, and body size of the S.
paramamosain larvae. An analysis of the BMP pathway
gene expression during the molting phase revealed that
the BMP2, BMPR2, and Smad1 genes displayed cyclic
expression patterns, while ACVR1, BMP7, and BMPRIB
maintained consistent expression levels throughout the
molting cycle. Additionally, the expression levels of
BMP2, BMPR2, and Smad1 in the inhibition group were
significantly lower compared to those in the control
group. Furthermore, the inhibition of the BMP pathway
led to an increase in the expression of MIH during the
intermolt period and a decrease in the expression of
EcR during the premolt period. These findings
demonstrate that the BMP signaling pathway affects the
molting of Scylla paramamosain juvenile crabs by
influencing the expression of the critical genes MIH
and ECR during molting, offering valuable data for
functional research on the BMP signaling pathway in
crustaceans.",
acknowledgement = ack-nhfb,
journal-URL = "https://www.mdpi.com/journal/fishes",
}
